JP5426850B2 - インフルエンザワクチンのアジュバント - Google Patents
インフルエンザワクチンのアジュバント Download PDFInfo
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- JP5426850B2 JP5426850B2 JP2008210484A JP2008210484A JP5426850B2 JP 5426850 B2 JP5426850 B2 JP 5426850B2 JP 2008210484 A JP2008210484 A JP 2008210484A JP 2008210484 A JP2008210484 A JP 2008210484A JP 5426850 B2 JP5426850 B2 JP 5426850B2
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- Prior art keywords
- strain
- positive
- lactic acid
- influenza vaccine
- adjuvant
- Prior art date
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Description
すなわち、本発明は、ラクトバチルス・プランタラムAYA株を有効成分として含有し、インフルエンザワクチンの経口投与前に摂取される、インフルエンザワクチンのアジュバントを提供するものである。
ラクトバチルス・プランタラムAYA株は、パン生地から分離された株であり、オリエンタル酵母工業株式会社(東京都板橋区小豆沢3丁目6番10号)により、2006年11月29日付で独立行政法人産業技術総合研究所特許生物寄託センター(茨城県つくば市東1丁目1番地1中央第6)に、受託番号FERM P−21106として寄託されている。
なお、ラクトバチルス・プランタラムAYA株は、特開2008−179595号公報に記載された乳酸菌であり、これを用いて麦を乳酸菌発酵させた産物を有効成分とする脂肪細胞分化促進剤も同公開公報に記載されている。さらに、ラクトバチルス・プランタラムAYA株は、特願2007−39019号に係る腸管免疫力増強剤の有効成分としても提案されているが、ラクトバチルス・プランタラムAYA株単体がインフルエンザワクチンのアジュバントとしての効能を持つことは、本発明者らによって初めて見い出されたことである。
MRS液体培地(DIFCO社)を用いて、30℃、18時間培養したときの菌の形態(1)菌の形態 桿菌
(2)グラム染色 陽性
(3)運動性 なし
(4)胞子 なし
(5)カタラーゼ なし
(6)通性嫌気性
(7)ブドウ糖の代謝 50%以上乳酸に転換する
(8)生育温度範囲 15℃、30℃および35℃では生育を認めるが、45℃では生育を認めない
(9)乳酸発酵 ホモ型
(10)乳酸の旋光性 DL
(11)炭水化物の発酵性 グリセロールは陽性、D−アラビノースは陰性、L−アラビノースは陽性、リボースは陽性、D−キシロースは陰性、ガラクトースは陽性、グルコースは陽性、フルクトースは陽性、マンノースは陽性、ラムノースは陽性、マンニトールは陽性、ソルビトールは陽性、αメチルDグルコシドは陰性、アミグダリンは陽性、エスクリンは陽性、サリシンは陽性、セロビオースは陽性、マルトースは陽性、ラクトースは陽性、メリビオースは陽性、シュクロースは陽性、トレハロースは陽性、イヌリンは陰性、メレジトースは陽性、ラフィノースは陽性、スターチは陰性、グルコン酸は陽性。
(乳酸菌試料の調製)
ラクトバチルス・プランタラムAYA株を、10μg/mlシクロヘキシミドを含むMRS(de Man−Rogosa−Sharpe)培地を用いて37℃で48時間培養した。その後、遠心分離によって集菌し、滅菌水で3回洗浄した後、滅菌水に懸濁し、121℃で30分間オートクレーブ処理し、これを凍結乾燥して乳酸菌試料を得た。
その結果、図1に示すように、乳酸菌試料投与群の回腸側のPR8特異的IgA濃度は、対照群に比べて有意(危険率5%)に上昇していた。
BALB/cマウス(各群6匹)に餌を42日間投与した後、インフルエンザウイルスPR8株をフォルムアミドで不活化したワクチン(150μg/200μl 0.2M NaHCO3)を経口投与した。餌として、対照群にはAIN97をペレット化し、γ線滅菌したものを投与し、乳酸菌試料投与群にはAIN97に上記乳酸菌試料を5質量%含有させたものをペレット化し、γ線滅菌したものを投与した。ワクチン投与後14日目にインフルエンザウイルスPR8株20LD50(84000TCID50/20μl)をBALB/cマウスに経鼻感染させ、その3日目に解剖し、小腸を切除し、大腸側(回腸側)の洗浄液中のPR8特異的IgA濃度をELISA法により測定した。
その結果、図2に示すように、乳酸菌試料投与群の回腸側のPR8特異的IgA濃度は、対照群に比べて有意に上昇していた。
Claims (1)
- ラクトバチルス・プランタラムAYA株(受託番号FERM P−21106)を有効成分として含有し、インフルエンザワクチンの経口投与前に摂取される、インフルエンザワクチンのアジュバント。
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