JP5414147B2 - 立体的高解像度結像 - Google Patents
立体的高解像度結像 Download PDFInfo
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- JP5414147B2 JP5414147B2 JP2006505064A JP2006505064A JP5414147B2 JP 5414147 B2 JP5414147 B2 JP 5414147B2 JP 2006505064 A JP2006505064 A JP 2006505064A JP 2006505064 A JP2006505064 A JP 2006505064A JP 5414147 B2 JP5414147 B2 JP 5414147B2
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- 238000003384 imaging method Methods 0.000 title claims description 7
- 238000000034 method Methods 0.000 claims description 45
- 239000000523 sample Substances 0.000 claims description 43
- 230000003287 optical effect Effects 0.000 claims description 42
- 239000000126 substance Substances 0.000 claims description 35
- 238000005259 measurement Methods 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 11
- 238000012360 testing method Methods 0.000 claims description 10
- 238000004020 luminiscence type Methods 0.000 claims description 5
- 230000003595 spectral effect Effects 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 4
- 239000012472 biological sample Substances 0.000 claims description 2
- 230000001427 coherent effect Effects 0.000 claims description 2
- 238000005401 electroluminescence Methods 0.000 claims description 2
- 108091006047 fluorescent proteins Proteins 0.000 claims description 2
- 102000034287 fluorescent proteins Human genes 0.000 claims description 2
- 238000010353 genetic engineering Methods 0.000 claims description 2
- 230000000737 periodic effect Effects 0.000 claims description 2
- 230000010287 polarization Effects 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 description 32
- 230000005284 excitation Effects 0.000 description 10
- 238000000799 fluorescence microscopy Methods 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 3
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 3
- 239000005090 green fluorescent protein Substances 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 230000000153 supplemental effect Effects 0.000 description 2
- 241000242762 Anemonia sulcata Species 0.000 description 1
- 108050002220 Green fluorescent protein, GFP Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
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- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 229940069435 retaine Drugs 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Microscoopes, Condenser (AREA)
Description
物質は無論、試料の結像する組織を現わすことができる。さもなければ、試料の組織は人工的に物質によりマークされなければならない。
新たな方法の好ましい実施態様は従属請求項に記載されている。
・分子の立体配座状態 ・分子の構造式
・分子内部の原子の立体的配列
・分子内部の結合の立体的配列
・一分子に別の原子或いは分子の付加
・原子及び/又は分子のグループ化
・分子の立体的配向
・互いに隣接した分子の配向
・多数の分子及び/又は原子により形成された配列
2....第二状態
3....変換信号
4....テスト信号
5....測定信号
6....蛍光励起部
7....試料
8....蛍光阻止手段
9....立体的領域
10...カメラ
11、12...照射分割器
13...対物レンズ
14...照射
15...干渉マスター15
Claims (18)
- ・光学的切換信号により繰り返して第一光学特性をもつ第一状態から第二光学特性をもつ第二状態へ移行でき、第二状態から第一状態へ戻されることができる一グループの物質から物質を選定し、
・試料の領域の物質を切換信号によって第二状態へ移行し、この際に定義された領域が故意に省略され、
・物質が第二状態へ移行される領域の他に故意に省略された領域を包含する記録領域用の第一状態の物質に付属される光学測定信号を記録する、
これら工程によって試料の物質でマークを付けられた組織を立体的に高解像する結像方法において、
物質が下位グループの物質から選定され、両状態(1、2)が少なくとも次の判断基準の一つに関して区別される:
・分子の立体配座状態
・分子の構造式
・分子内部の原子の立体的配列
・分子内部の結合の立体的配列
・一分子に別の原子或いは分子の付加
・原子及び/又は分子のグループ化
・分子の立体的配向
・互いに隣接した分子の配向
・多数の分子及び/又は原子により形成された配列;
そして・試料(7)の領域の物質を切換信号(3)により第二状態(2)へ移行し、この際にそれぞれの一つの定義された領域(9)が故意に省略され、
・第一状態の物質に付属されている光学測定信号(5)をそれぞれ故意に省略された定義された領域(9)を包含する記録領域のために記録し、
・物質を第一状態へ移行する工程が試料(7)の異なる定義された領域のために周期的順序で相前後して実施されるので、試料が故意に省略された領域を走査され、そして
物質は、スイッチ信号により第二状態(2)から第一状態(1)へ切換できるグループの物質から選定され、そしてスイッチ信号は切換信号(3)との前或いは同時に試料(7)に伝達されることを特徴とする方法。 - 第二状態(2)の寿命が1nsより長いことを特徴とする請求項1に記載の方法。
- 物質の両状態(1、2)の異なる光学特性は異なるスペクトル特性であることを特徴とする請求項1或いは2に記載の方法。
- 第一光学特性は第二光学特性に比べてテスト信号用の異なる吸収性を有することを特徴とする請求項3に記載の方法。
- 第一光学特性は第二光学特性に比べて蛍光、燐光、電気ルミネセンスと化学ルミネセンスから成る異なるルミネセンスを有することを特徴とする請求項3に記載の方法。
- 物質の状態の異なる光学特性は異なる極化特性であることを特徴とする請求項1或いは2に記載の方法。
- 物質と切換信号(3)は、切換信号(3)の強度が飽和限界値を超過する至る所において完全に物質の第二状態(2)に調整するように相前後して適合されることを特徴とする請求項1乃至6のいずれか一項に記載の方法。
- 切換信号(3)の強度は故意に省略された領域(9)の外部の全記録領域にて飽和限界値を超過し、そして故意に省略された立体的領域(9)が切換信号(3)の局部的最小強度であることを特徴とする請求項7に記載の方法。
- 切換信号(3)の局部的最小強度は切換信号(3)の干渉性光線(14)の干渉模様(15)の零点であることを特徴とする請求項8に記載の方法。
- スイッチ信号は記録領域を閉じ込める大きい領域を介して試料(7)に伝達されることを特徴とする請求項8或いは9に記載の方法。
- 切換信号(3)後にテスト信号が試料(7)に伝達されることを特徴とする請求項1乃至10のいずれか一項に記載の方法。
- テスト信号は故意に省略された領域(9)を閉じ込める大きい領域を介して試料(7)に伝達されることを特徴とする請求項11に記載の方法。
- ルミネセンスはスイッチ信号によって励起され、この際にスイッチ信号は切換信号(3)の伝達中に試料(7)に伝達されることを特徴とする請求項5に記載の方法。
- 第一状態の物質に付属されている複数光学測定信号は同時に、物質が第二状態に変換される領域以外にそれぞれに故意に省略された領域(9)を包含する複数の記録領域のために互いに分離されて記録されることを特徴とする請求項1乃至12のいずれか一項に記載の方法。
- 物質は蛋白質を包含する下位グループから選定されることを特徴とする請求項1乃至14のいずれか一項に記載の方法。
- 物質は蛍光蛋白質を包含する下位グループから選定されることを特徴とする請求項15に記載の方法。
- 組織にマークを付けるために物質は遺伝子工学的方法で生物学的試料(7)に取り付けられることを特徴とする請求項1乃至16のいずれか一項に記載の方法。
- 請求項1乃至16のいずれか一項に記載の方法を実施する際に蛍光顕微鏡を使用する使用方法。
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10317613.6 | 2003-04-13 | ||
DE10317613 | 2003-04-13 | ||
US10/420,896 | 2003-04-22 | ||
US10/420,896 US7064824B2 (en) | 2003-04-13 | 2003-04-22 | High spatial resoulution imaging and modification of structures |
DE10325460A DE10325460A1 (de) | 2003-04-13 | 2003-06-05 | Räumlich hochauflösendes Abbilden |
DE10325460.9 | 2003-06-05 | ||
PCT/EP2004/003767 WO2004090617A2 (de) | 2003-04-13 | 2004-04-08 | Räumlich hochauflösendes abbilden |
Publications (3)
Publication Number | Publication Date |
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JP2007524071A JP2007524071A (ja) | 2007-08-23 |
JP2007524071A5 JP2007524071A5 (ja) | 2011-07-21 |
JP5414147B2 true JP5414147B2 (ja) | 2014-02-12 |
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JP2006505064A Expired - Lifetime JP5414147B2 (ja) | 2003-04-13 | 2004-04-08 | 立体的高解像度結像 |
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EP (1) | EP1616216B1 (ja) |
JP (1) | JP5414147B2 (ja) |
WO (1) | WO2004090617A2 (ja) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2004090617A2 (de) * | 2003-04-13 | 2004-10-21 | Max-Planck-Gesselschaft Zur Förderung Der Wissenschaften E.V. | Räumlich hochauflösendes abbilden |
EP2453238B1 (en) | 2005-05-23 | 2016-12-21 | Harald F. Hess | Optical microscopy with phototransformable optical labels |
ATE441103T1 (de) † | 2005-07-22 | 2009-09-15 | Zeiss Carl Microimaging Gmbh | Auflísungsgesteigerte lumineszenz-mikroskopie |
DE102006021317B3 (de) | 2006-05-06 | 2007-10-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
DE102006026203A1 (de) * | 2006-05-31 | 2007-12-06 | Carl Zeiss Microimaging Gmbh | Verfahren zum räumlich hochauflösenden Abbilden |
US7776613B2 (en) * | 2006-08-07 | 2010-08-17 | President And Fellows Of Harvard College | Sub-diffraction image resolution and other imaging techniques |
US7838302B2 (en) | 2006-08-07 | 2010-11-23 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution and other imaging techniques |
DE102006045607A1 (de) * | 2006-09-25 | 2008-03-27 | Leica Microsystems Cms Gmbh | Verfahren zur räumlich hochauflösenden Untersuchung von einer mit einer fluoreszierenden Substanz markierten Struktur einer Probe |
US8217992B2 (en) | 2007-01-11 | 2012-07-10 | The Jackson Laboratory | Microscopic imaging techniques |
DE102007011305A1 (de) | 2007-03-06 | 2008-09-11 | Leica Microsystems Cms Gmbh | Vorrichtung und Verfahren zur Strahljustage in einem optischen Strahlengang |
DE102007033737A1 (de) | 2007-07-18 | 2009-01-22 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zum Bestimmen eines Messwerts auf der Basis von Einzelmolekülereignissen |
US8564792B2 (en) | 2007-12-21 | 2013-10-22 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution in three dimensions |
DE102008011993A1 (de) | 2008-02-29 | 2009-09-10 | Leica Microsystems Cms Gmbh | Synchronisierte Bildgebung mittels optischer Verfahren und Rasterkraftmikroskopie |
US7772569B2 (en) | 2008-04-01 | 2010-08-10 | The Jackson Laboratory | 3D biplane microscopy |
DE102009015341A1 (de) | 2009-03-27 | 2010-10-07 | Carl Zeiss Ag | Verfahren und Vorrichtungen zur optischen Untersuchung von Proben |
WO2013007726A1 (en) * | 2011-07-11 | 2013-01-17 | Facultes Universitaires Notre-Dame De La Paix | Method for high resolution sum-frequency generation and infrared microscopy |
JP6396324B2 (ja) | 2013-01-04 | 2018-09-26 | ユニバーシティ オブ リメリック | 差動赤外線ナノ顕微鏡法システムおよび方法 |
DE102016104651A1 (de) | 2015-03-24 | 2016-09-29 | Abberior Instruments Gmbh | Verfahren und Rasterfluoreszenzlichtmikroskop zum dreidimensional hochauflösenden Abbilden einer mit Fluorophoren markierten Struktur einer Probe |
Family Cites Families (10)
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---|---|---|---|---|
US4288861A (en) * | 1977-12-01 | 1981-09-08 | Formigraphic Engine Corporation | Three-dimensional systems |
US5289407A (en) * | 1991-07-22 | 1994-02-22 | Cornell Research Foundation, Inc. | Method for three dimensional optical data storage and retrieval |
ATE204086T1 (de) * | 1994-02-01 | 2001-08-15 | Hell Stefan | Vorrichtung und verfahren zum optischen messen eines probenpunktes einer probe mit hoher ortsauflösung |
US6046925A (en) * | 1997-04-14 | 2000-04-04 | The Regents Of The University Of California | Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins |
JPH1195120A (ja) * | 1997-09-19 | 1999-04-09 | Olympus Optical Co Ltd | 顕微鏡の観察方法 |
JP3350442B2 (ja) * | 1998-04-09 | 2002-11-25 | 科学技術振興事業団 | 顕微鏡システム |
JP4551528B2 (ja) * | 2000-03-23 | 2010-09-29 | オリンパス株式会社 | 二重共鳴吸収顕微鏡 |
JP2001272343A (ja) * | 2000-03-23 | 2001-10-05 | Olympus Optical Co Ltd | 二重共鳴吸収顕微鏡 |
US20030174560A1 (en) * | 2002-02-26 | 2003-09-18 | Klaus-Hermann Dahmen | Photochromic compounds for molecular switches and optical memory |
WO2004090617A2 (de) * | 2003-04-13 | 2004-10-21 | Max-Planck-Gesselschaft Zur Förderung Der Wissenschaften E.V. | Räumlich hochauflösendes abbilden |
-
2004
- 2004-04-08 WO PCT/EP2004/003767 patent/WO2004090617A2/de active Application Filing
- 2004-04-08 JP JP2006505064A patent/JP5414147B2/ja not_active Expired - Lifetime
- 2004-04-08 EP EP04726457.7A patent/EP1616216B1/de not_active Expired - Lifetime
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Publication number | Publication date |
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WO2004090617A2 (de) | 2004-10-21 |
EP1616216A2 (de) | 2006-01-18 |
EP1616216B1 (de) | 2018-11-07 |
JP2007524071A (ja) | 2007-08-23 |
WO2004090617A3 (de) | 2007-02-08 |
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