JP5196094B2 - Feed containing γ-aminobutyric acid-containing composition and method for producing the same - Google Patents
Feed containing γ-aminobutyric acid-containing composition and method for producing the same Download PDFInfo
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- JP5196094B2 JP5196094B2 JP2006155356A JP2006155356A JP5196094B2 JP 5196094 B2 JP5196094 B2 JP 5196094B2 JP 2006155356 A JP2006155356 A JP 2006155356A JP 2006155356 A JP2006155356 A JP 2006155356A JP 5196094 B2 JP5196094 B2 JP 5196094B2
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- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 title claims description 198
- 239000000203 mixture Substances 0.000 title claims description 113
- 229960003692 gamma aminobutyric acid Drugs 0.000 title claims description 100
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 title claims description 98
- 238000004519 manufacturing process Methods 0.000 title claims description 23
- 240000007594 Oryza sativa Species 0.000 claims description 98
- 235000007164 Oryza sativa Nutrition 0.000 claims description 98
- 235000009566 rice Nutrition 0.000 claims description 97
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- 235000011453 Vigna umbellata Nutrition 0.000 claims description 48
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 46
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- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 24
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- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 20
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- 240000001929 Lactobacillus brevis Species 0.000 claims description 14
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- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
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Description
本発明は、γ-アミノ酪酸含有組成物を含む飼料とその製造方法とに関する。 The present invention relates to a feed containing a γ-aminobutyric acid-containing composition and a method for producing the same.
発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地で乳酸菌を培養し固−液分離し、γ-アミノ酪酸含有組成物の製造する際に生じた液体部分及び固形部分、すなわちγ-アミノ酪酸含有組成物（特許文献１）は、従来、人の飲食物として利用されていたが、家畜の飼料としては利用されていない。 Solid-liquid cultivating lactic acid bacteria in a medium containing at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof The liquid part and solid part produced when the γ-aminobutyric acid-containing composition is separated and produced, that is, the γ-aminobutyric acid-containing composition (Patent Document 1) has been conventionally used as a human food and drink. It is not used as livestock feed.
一方、畜産分野（畜産農家）では、日本国内で生産されたものを原料として、さらに日本国内で加工処理された国産飼料への要求が強い。
しかしながら、γ-アミノ酪酸含有組成物は、家畜における嗜好性および飼料特性が不明のため、飼料原料としての利用が困難であった。
On the other hand, in the field of livestock (livestock farmers), there is a strong demand for domestic feed that is produced in Japan and processed in Japan.
However, the γ-aminobutyric acid-containing composition has been difficult to use as a feed raw material because the palatability and feed characteristics in livestock are unknown.
上記したように、従来、γ-アミノ酪酸含有組成物は、飼料として利用可能な栄養成分を含んでいるにも拘わらず、家畜における嗜好性および飼料特性が不明のため飼料原料として利用できなかった。
本発明は、従来存在しなかった、γ-アミノ酪酸含有組成物を含む飼料を提供することを目的とするものである。
即ち、家畜における嗜好性および飼料特性に優れた、γ-アミノ酪酸含有組成物を含む飼料を提供することを目的とするものである。
As described above, γ-aminobutyric acid-containing compositions have been conventionally unusable as feed ingredients because of their unclear palatability and feed characteristics in spite of containing nutrients that can be used as feed. .
An object of the present invention is to provide a feed containing a γ-aminobutyric acid-containing composition that has not existed conventionally.
That is, an object of the present invention is to provide a feed containing a γ-aminobutyric acid-containing composition that is excellent in palatability and feed characteristics in livestock.
本発明者らは、上記課題を解決するため鋭意研究を重ねた結果、γ-アミノ酪酸含有組成物と、糖或いはデンプン含量の高い、液体又は固体原料と、から実質的になるγ-アミノ酪酸含有組成物を含む飼料が目的を達成しうることを見出し、この知見に基づいて本発明を完成するに至った。
請求項1に係る本発明は、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものを加水混合し、pH4.6以下に調製し、常圧で加熱滅菌し、グルタミン酸もしくはその塩を添加し、その後乳酸菌を接種して培養することによりγ-アミノ酪酸含有組成物を得、；
当該γ-アミノ酪酸含有組成物と、トウモロコシ粉末を、攪拌混合することを特徴とする、；γ-アミノ酪酸含有組成物を含む飼料の製造方法を提供するものである。
請求項2に係る本発明は、前記トウモロコシ粉末が、粒径4mm以下の乾燥粉末である、請求項1に記載の方法を提供するものである。
請求項3に係る本発明は、前記乳酸菌が、Lactobacillus brevis IFO12005株である、請求項1又は2に記載の方法を提供するものである。
請求項4に係る本発明は、回転刃を有するブレンダー或いはカッターミキサーを用いて撹拌混合する、請求項1〜3のいずれかに記載の方法を提供するものである。
As a result of intensive studies to solve the above problems, the present inventors have found that a γ-aminobutyric acid substantially comprising a γ-aminobutyric acid-containing composition and a liquid or solid raw material having a high sugar or starch content. It discovered that the feed containing a containing composition can achieve the objective, and came to complete this invention based on this knowledge.
The present invention according to claim 1 is a mixture of at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat , pH 4. 6 or less, heat-sterilized at normal pressure, added glutamic acid or a salt thereof, and then inoculated with lactic acid bacteria and cultured to obtain a γ-aminobutyric acid-containing composition;
The γ-aminobutyric acid-containing composition and corn powder are mixed by stirring ; and a method for producing a feed containing the γ-aminobutyric acid-containing composition is provided.
The present invention according to claim 2 provides the method according to claim 1, wherein the corn powder is a dry powder having a particle size of 4 mm or less.
The present invention according to claim 3 provides the method according to claim 1 or 2, wherein the lactic acid bacteria is Lactobacillus brevis IFO12005 strain.
The present invention according to claim 4 provides the method according to any one of claims 1 to 3 , wherein stirring and mixing are performed using a blender or a cutter mixer having a rotary blade.
本発明によれば、従来存在しなかった、γ-アミノ酪酸含有組成物を含む飼料が提供される。
従って、γ-アミノ酪酸含有組成物の家畜飼料としての有用性が示されたことになる。
即ち、本発明によれば、γ-アミノ酪酸含有組成物の飼料特性および飼料価値の評価と、γ-アミノ酪酸含有組成物を含む飼料の組成および製造方法を検討することで、γ-アミノ酪酸含有組成物の家畜飼料としての有用性を示された。
本発明によれば、畜産分野でのγ-アミノ酪酸含有組成物を含む飼料としての利用および、飼料製造企業或いは団体等に直接技術移転が可能となる。
According to the present invention, there is provided a feed comprising a γ-aminobutyric acid-containing composition that has not existed conventionally.
Therefore, the usefulness as a livestock feed of the composition containing γ-aminobutyric acid was shown.
That is, according to the present invention, γ-aminobutyric acid can be obtained by evaluating the feed characteristics and feed value of the γ-aminobutyric acid-containing composition and examining the composition and production method of the feed containing the γ-aminobutyric acid-containing composition. The usefulness of the containing composition as a livestock feed was shown.
According to the present invention, use as a feed containing a γ-aminobutyric acid-containing composition in the field of livestock production and direct technology transfer to a feed manufacturing company or organization can be realized.
本発明は、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌の培養物を有するγ-アミノ酪酸含有組成物と、糖或いはデンプン含量の高い、液体又は固体原料と、から実質的になるγ-アミノ酪酸含有組成物を含む飼料である。
本発明において用いるγ-アミノ酪酸含有組成物は、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌の培養物を有するもの、具体的には例えばラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株の培養物を有するものである。
The present invention relates to lactic acid bacteria in a medium containing at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof. A feed comprising a γ-aminobutyric acid-containing composition substantially comprising a γ-aminobutyric acid-containing composition having a culture and a liquid or solid raw material having a high sugar or starch content.
The γ-aminobutyric acid-containing composition used in the present invention is a germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or Those having a culture of lactic acid bacteria in a medium containing the salt, specifically, for example, having a culture of Lactobacillus brevis IFO12005 strain.
このγ-アミノ酪酸含有組成物は、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地で乳酸菌、具体的には例えばラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株を培養し、必要に応じて固−液分離することにより製造することができる。
本発明においては、γ-アミノ酪酸含有組成物としては、固−液分離した後の固形部分を用いることが特に好ましい。
This γ-aminobutyric acid-containing composition comprises at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof. It can be produced by culturing a lactic acid bacterium, specifically, for example, Lactobacillus brevis IFO12005 strain in a medium containing it, and solid-liquid separation as necessary.
In the present invention, as the γ-aminobutyric acid-containing composition, it is particularly preferable to use a solid portion after solid-liquid separation.
本発明において培地に用いる発芽玄米糠としては、どの様な発芽玄米の糠でも良い。米糠も同様であり、無洗米製造時に発生する無洗米粕（肌糠）も含む。但し、精米後、時間が経過すると米糠臭の発生、微生物の増加が懸念されるので、使用までは冷蔵保管が好ましい。
米糠は、細かくいうと、米の一番外側の部分から、米の芯に向かって順に、赤糠、中糠、白糠に区分され、本発明ではいずれも用いることができ、勿論全体としての米糠を用いることができる。
また、本発明においては、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮も培地として用いることができる。
ここで脱脂米糠は、前記した米糠を常法により脱脂したものである。
本発明においては、上記した発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた１種のものを単独で、或いはこれらを２種以上併用することができる。
本発明においては、少なくとも発芽玄米糠を用いることが好ましいことから、特に発芽玄米糠単独、又は発芽玄米糠と米糠の混合物が好ましい。
それ以外の米、脱脂米糠、ふすま、大豆種皮、小豆種皮、竹小豆種皮を培地として用いる場合では、酵素処理による培地のアミノ酸等の栄養源の増加、もしくは酵母エキスを添加することが好ましい。
The germinated brown rice bran used in the medium in the present invention may be any germinated brown rice bran. The same is true for rice bran, including non-washed rice bran (skin candy) generated during the production of non-washed rice. However, refrigerated storage is preferred until use because rice odor and generation of microorganisms are feared over time after milling.
Rice bran is divided into red rice bran, medium rice bran, and white rice bran in order from the outermost part of rice toward the core of the rice, and any of these can be used in the present invention. Can be used.
In the present invention, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat can also be used as the medium.
Here, the defatted rice bran is obtained by defatting the aforementioned rice bran by a conventional method.
In the present invention, the above-mentioned germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat are used alone or in combination of two or more thereof. Can be used together.
In the present invention, since it is preferable to use at least germinated brown rice bran, germinated brown rice bran alone or a mixture of germinated brown rice bran and rice bran is particularly preferable.
When other rice, defatted rice bran, bran, soybean seed coat, red bean seed coat, and bamboo red bean seed coat are used as the medium, it is preferable to increase the nutrient sources such as amino acids in the medium by enzyme treatment or to add yeast extract.
次に、γ-アミノ酪酸含有組成物を製造するにあたっては、培地にさらにグルタミン酸もしくはその塩を用いる。グルタミン酸の塩としては、具体的にはナトリウム塩、カリウム塩、カルシウム塩、マグネシウム塩等が挙げられる。
培地としては、上記した発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地であればよく、さらに通常、水を含むものである。この他、必要に応じて培地として米糖化液、甘酒、麹エキス等を用いることもできる。
Next, in producing the γ-aminobutyric acid-containing composition, glutamic acid or a salt thereof is further used in the medium. Specific examples of the salt of glutamic acid include sodium salt, potassium salt, calcium salt, magnesium salt and the like.
The medium may be at least one selected from the group consisting of the germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof. What is necessary is just to contain water normally. In addition, rice saccharified solution, amazake, koji extract and the like can be used as a medium as necessary.
また、使用する乳酸菌としては、γ-アミノ酪酸（GABA）生産乳酸菌であればどのような菌でも用いることができるが、GABA生産乳酸菌の中でも特にラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株が好適に用いられる。
このラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株を用いることによって、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のもののみを栄養源とすることにより、添加するグルタミン酸もしくはその塩をγ-アミノ酪酸に変換すると共に、菌体生産が可能である。
発芽玄米糠では乳酸菌が10の8乗オーダーまで増殖可能であり、種培養用の培地としても充分な菌体量である。
As the lactic acid bacterium to be used, any lactic acid bacterium producing γ-aminobutyric acid (GABA) can be used. Among the lactic acid bacteria producing GABA, Lactobacillus brevis IFO12005 strain is preferably used. It is done.
By using this Lactobacillus brevis IFO12005 strain, only at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat As a nutrient source, glutamic acid or a salt thereof to be added is converted into γ-aminobutyric acid, and cell production is possible.
In germinated brown rice bran, lactic acid bacteria can grow to the order of 10 to the 8th power, and the amount of cells is sufficient as a medium for seed culture.
本発明で用いるγ-アミノ酪酸含有組成物は、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌、具体的には例えばラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株の培養物を有するものである。
添加するグルタミン酸もしくはその塩、特にグルタミン酸ナトリウム（MSG）を効率よくγ-アミノ酪酸に変換するには、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものに対し、重量で0.1〜30％、好ましくは1〜20%のグルタミン酸もしくはその塩、特にMSGを加え、水を発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものの0.5〜20倍、好ましくは２〜８倍重量加え、ラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株を接種し、培養することが望ましい。
The γ-aminobutyric acid-containing composition used in the present invention is germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or It has a lactic acid bacterium in a medium containing the salt, specifically, for example, a culture of Lactobacillus brevis IFO12005 strain.
To efficiently convert added glutamic acid or its salt, especially sodium glutamate (MSG) into γ-aminobutyric acid, it consists of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat To at least one selected from the group, 0.1 to 30% by weight, preferably 1 to 20% glutamic acid or a salt thereof, especially MSG is added, and water is germinated. Brown rice bran, rice bran, rice, defatted rice bran, 0.5 to 20 times, preferably 2 to 8 times the weight of at least one selected from the group consisting of bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat, and inoculated with Lactobacillus brevis IFO12005 strain, It is desirable to culture.
このときの培養は静置が望ましく、培養温度は1〜40℃、好ましくは3〜35℃であり、培養時間は1日〜30日、好ましくは2日から14日である。
具体的には例えば、発芽玄米糠5g、MSG 0.5g、水40mlの混合液を121℃で15分間オートクレーブ滅菌後、ラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株を接種し、30℃で7日間培養することにより、固形分中のγ-アミノ酪酸含有量は4.22%に達する。
The culture at this time is desirably left stationary, the culture temperature is 1 to 40 ° C., preferably 3 to 35 ° C., and the culture time is 1 to 30 days, preferably 2 to 14 days.
Specifically, for example, a mixture of germinated brown rice bran 5g, MSG 0.5g, and water 40ml is autoclaved at 121 ° C for 15 minutes, inoculated with Lactobacillus brevis IFO12005 strain, and cultured at 30 ° C for 7 days As a result, the content of γ-aminobutyric acid in the solid content reaches 4.22%.
このように発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地で乳酸菌ラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株を培養することにより、目的とするγ-アミノ酪酸含有組成物が得られる。
培養後、必要に応じて固−液分離することにより、固形部分であるγ-アミノ酪酸含有組成物と共に、液体部分であるγ-アミノ酪酸高含有水が得られる。固−液分離は、遠心分離または濾過によって行われる。γ-アミノ酪酸高含有水の脱色・脱臭は活性炭、ゼオライト、合成吸着剤などにより行うことができる。
また、培養液の濾過性を向上させるために、α−アミラーゼ、グルコアミラーゼなどの多糖分解酵素、蛋白分解酵素、脂質分解酵素を培養前又は培養終了後に用いることができる。培養前に、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものに、多糖分解酵素、蛋白分解酵素及び脂質分解酵素のうちの少なくとも１種を作用させることにより、糖、アミノ酸、有機酸を乳酸菌の栄養源として富化することもできる。
Thus, the lactic acid bacteria Lactobacillus brevis in a medium containing at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof By culturing ( Lactobacillus brevis ) IFO12005 strain, the intended γ-aminobutyric acid-containing composition is obtained.
After culturing, solid-liquid separation is performed as necessary to obtain γ-aminobutyric acid-rich water as a liquid portion together with a γ-aminobutyric acid-containing composition as a solid portion. Solid-liquid separation is performed by centrifugation or filtration. Decolorization and deodorization of water with a high content of γ-aminobutyric acid can be performed using activated carbon, zeolite, synthetic adsorbent, or the like.
In addition, in order to improve the filterability of the culture solution, polysaccharide-degrading enzymes such as α-amylase and glucoamylase, proteolytic enzymes, and lipolytic enzymes can be used before or after culturing. Before culturing, at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat, and bamboo red bean seed coat, polysaccharide-degrading enzyme, proteolytic enzyme and lipolytic enzyme By acting at least one of the enzymes, sugars, amino acids, and organic acids can be enriched as a nutrient source for lactic acid bacteria.
なお、培養に先立ち、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものの加水混合液に酸添加し、ｐＨ４．６以下としておくことにより、常圧下での加熱殺菌が可能となる。このとき加える酸としては鉱酸でも有機酸でも良い。好ましくはクエン酸、乳酸等の不揮発性の有機酸が望ましい。
このように発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものの加水混合液、つまり発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のもの、水の混合液に、乳酸などの酸を加え、ｐＨを４．６以下に調整することにより、常圧、100℃以下の加熱条件で殺菌できる。この結果、加圧滅菌装置が不要となる。なお、このとき、添加するグルタミン酸もしくはその塩は別途滅菌処理したものを用いるとよい。
Prior to cultivation, acid is added to a hydrated mixture of at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat, and pH 4. By setting it to 6 or less, heat sterilization under normal pressure becomes possible. The acid added at this time may be a mineral acid or an organic acid. Nonvolatile organic acids such as citric acid and lactic acid are preferred.
In this way, a hydrous mixture of at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat, that is, germinated brown rice bran, rice bran, rice, Add acid such as lactic acid to a mixture of water and at least one selected from the group consisting of defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat, and adjust the pH to 4.6 or lower. Can be sterilized under normal pressure and heating conditions of 100 ° C. or lower. As a result, an autoclave is not required. At this time, glutamic acid or a salt thereof to be added may be sterilized separately.
具体的には、殺菌、冷却後、MSGを糠重量に対し0.1〜20%を加え、ラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株を接種し、30℃で7日間培養することにより、固形分中のγ-アミノ酪酸含有量は発芽玄米糠試験区で6.19〜7.02%、米糠試験区で6.27〜7.12%となった。特にMSGの変換率を95%以上にするにはMSGの添加量は20％以下が良い。
このようにして、γ-アミノ酪酸含有組成物が得られる。
Specifically, after sterilization and cooling, 0.1 to 20% of MSG is added to the cocoon weight, inoculated with Lactobacillus brevis IFO12005 strain, and cultured at 30 ° C for 7 days, The content of γ-aminobutyric acid was 6.19 to 7.02% in the germinated brown rice bran test zone and 6.27 to 7.12% in the rice bran test zone. In particular, to increase the conversion rate of MSG to 95% or more, the addition amount of MSG should be 20% or less.
In this way, a γ-aminobutyric acid-containing composition is obtained.
本発明は、このような発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌の培養物を有するγ-アミノ酪酸含有組成物と、糖或いはデンプン含量の高い、液体又は固体原料と、から実質的になるγ-アミノ酪酸含有組成物を含む飼料である。
ここで糖或いはデンプン含量の高い、液体又は固体原料として具体的には、米粉糖化液、糖蜜、トウモロコシ粉末などを挙げることができる。トウモロコシ粉末としては、粒径が４ｍｍ以下程度に粉砕処理した乾燥トウモロコシ粉末が好ましい。
The present invention is a medium containing at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof. A feed comprising a γ-aminobutyric acid-containing composition having a culture of lactic acid bacteria, and a liquid or solid raw material having a high sugar or starch content.
Specific examples of the liquid or solid raw material having a high sugar or starch content include saccharified rice flour, molasses, and corn powder. As the corn powder, dry corn powder having a particle size of about 4 mm or less is preferable.
発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌の培養物を有するγ-アミノ酪酸含有組成物と、糖或いはデンプン含量の高い、液体又は固体原料の配合割合は、通常、前者１〜９９重量％に対して、後者９９〜１重量％、好ましくは前者６７重量％に対して、後者３３重量％である。 A culture of lactic acid bacteria in a medium containing at least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof The blending ratio of the γ-aminobutyric acid-containing composition and the liquid or solid raw material having a high sugar or starch content is usually 99 to 1% by weight, preferably 67% by weight of the latter with respect to 1 to 99% by weight of the former. On the other hand, the latter is 33% by weight.
なお、前記γ-アミノ酪酸含有組成物と米粉糖化液とを混合して得られる混合物の状態は、米粉糖化液含量が３０％（ｗ/ｗ）付近では粉末状である。
また、前記γ-アミノ酪酸含有組成物と糖蜜とを混合して得られる混合物の状態は、糖蜜含量が３０％（ｗ/ｗ）付近では粉末状であるが、約７０％（ｗ/ｗ）以上ではゲル状となる。
また、前記γ-アミノ酪酸含有組成物とトウモロコシ粉末とを混合して得られる混合物の状態は粉体である。
The state of the mixture obtained by mixing the γ-aminobutyric acid-containing composition and the rice flour saccharified solution is powdery when the rice flour saccharified solution content is around 30% (w / w).
In addition, the state of the mixture obtained by mixing the γ-aminobutyric acid-containing composition and molasses is powdery when the molasses content is around 30% (w / w), but is about 70% (w / w). Above, it becomes a gel.
In addition, the state of the mixture obtained by mixing the γ-aminobutyric acid-containing composition and corn powder is a powder.
このような発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌の培養物を有するγ-アミノ酪酸含有組成物と、糖或いはデンプン含量の高い、液体又は固体原料と、から実質的になるγ-アミノ酪酸含有組成物を含む飼料は、γ-アミノ酪酸含有組成物単体と比べて、格段に嗜好性が高いものとなっている。
このようなγ-アミノ酪酸含有組成物を含む飼料は、請求項1に記載したように、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌の培養物を有するγ-アミノ酪酸含有組成物と、糖或いはデンプン含量の高い、液体又は固体原料とを、攪拌混合することにより製造することができる。
攪拌混合は、好ましくは請求項4に記載したように、回転刃を有するブレンダー或いはカッターミキサーを用いて撹拌混合すればよく、好ましくは回転刃を有するブレンダー或いはカッターミキサーを用いて３,０００ｒｐｍ以上にて、２から５分間撹拌混合すればよい。
Culture of lactic acid bacteria in a medium containing at least one selected from the group consisting of such germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or a salt thereof A feed containing a γ-aminobutyric acid-containing composition consisting essentially of a γ-aminobutyric acid-containing composition having a product and a liquid or solid raw material having a high sugar or starch content is a γ-aminobutyric acid-containing composition alone Compared with, it has a much higher palatability.
The feed containing such a γ-aminobutyric acid-containing composition is from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat as described in claim 1. A composition containing γ-aminobutyric acid having a culture of lactic acid bacteria in a medium containing glutamic acid or a salt thereof and a liquid or solid raw material having a high sugar or starch content is stirred and mixed. Can be manufactured.
The stirring and mixing may be preferably performed by stirring and mixing using a blender or cutter mixer having a rotating blade as described in claim 4 , and preferably 3,000 rpm or more using a blender or cutter mixer having a rotating blade. And stirring and mixing for 2 to 5 minutes.
このようにして請求項１に記載した如き、発芽玄米糠、米糠、米、脱脂米糠、ふすま、大豆種皮、小豆種皮及び竹小豆種皮よりなる群から選ばれた少なくとも１種のものとグルタミン酸もしくはその塩を含む培地での乳酸菌の培養物を有するγ-アミノ酪酸含有組成物と、糖或いはデンプン含量の高い、液体又は固体原料と、から実質的になるγ-アミノ酪酸含有組成物を含む飼料が得られる。 Thus, as described in claim 1, germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat and glutamic acid or its A feed comprising a γ-aminobutyric acid-containing composition comprising a γ-aminobutyric acid-containing composition having a culture of lactic acid bacteria in a salt-containing medium and a liquid or solid raw material having a high sugar or starch content. can get.
以下に、実施例を挙げて本発明を詳しく説明するが、本発明はこれに限定されるものではない。 Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited thereto.
製造例１［米糠を用いたγ-アミノ酪酸含有組成物の製造］
米糠（あきたこまち精米90％）400kg、水1600Lをステンレス製発酵タンクに投入し、加熱減菌した。その後、40℃まで冷却し、MSG32kgを加えた。次に、グルコース2％、MSG1％、酵母エキス1％を含む培養液で前培養したラクトバチルス ブレビス（Lactobacillus brevis）IFO12005株培養液16Lを接種した。均一に攪拌後、30℃にて5日間静置培養した。培養液を加熱殺菌後、固-液分離を行い、得られたろ過残渣450kg（水分50％）をドラム式通風乾燥機によって乾燥し、1.30％（水分9.0％）のγ-アミノ酪酸含有組成物150kgを得た。
また、γ-アミノ酪酸含有組成物は以下の製造例２に記述する方法により、無洗米粕（肌糠）を用いて製造することもできた。
Production Example 1 [Production of γ-aminobutyric acid-containing composition using rice bran]
400 kg of rice bran (Akitakomachi polished rice 90%) and 1600 L of water were put into a stainless steel fermentation tank and sterilized by heating. Then, it cooled to 40 degreeC and added MSG32kg. Next, 16 L of Lactobacillus brevis IFO12005 strain culture solution pre-cultured with a culture solution containing 2% glucose, MSG 1%, and yeast extract 1% was inoculated. After stirring uniformly, static culture was performed at 30 ° C. for 5 days. After sterilization of the culture with heat, solid-liquid separation is performed, and the obtained filtration residue 450 kg (water content 50%) is dried by a drum-type ventilation dryer to obtain a 1.30% (water content 9.0%) γ-aminobutyric acid-containing composition. 150kg was obtained.
In addition, the γ-aminobutyric acid-containing composition could also be produced using non-washed rice bran (skin lees) by the method described in Production Example 2 below.
製造例２［無洗米粕（肌糠）を用いたγ-アミノ酪酸含有組成物の製造］
BG無洗米製造時に発生する肌糠2.5kg、水13.6Lを30Lジャーに採り良く混合した。混合液を乳酸にてpH4.5に調整後、市販酵素剤の液化アミラーゼ酵素剤、蛋白分解酵素剤、繊維分解酵素剤、脂肪分解酵素剤を1/1000（米糠重量に対して）を加え、攪拌しながら酵素処理を次のような昇温プログラムで行った。攪拌開始後６０分から９０分の間に４０℃から５５℃に上昇させ、さらに、攪拌開始してから２１０分後に１００℃に至り、１００℃で１０分間保持する様に温度を行い、その後、酵素処理液をオートクレーブ滅菌した（121℃、15分）。滅菌後、別途滅菌したMSG 600gを含む水溶液を加えた後、ラクトバチルス ブレビス（Lactobacillus brevis ）IFO12005の前培養液（生菌数1.0X109 cfu/ml) 1Lを接種し、30℃で攪拌（50rpm）した。培養2日後には添加したMSGは全てγ-アミノ酪酸に変換された。培養液を遠心分離により固液分離した。γ-アミノ酪酸濃度16.3mg/gの上清液19.2kgが得られた。また、沈殿部分は乾燥を行い、γ-アミノ酪酸濃度20.0mg/gの乾燥物860ｇが得られた。
Production Example 2 [Production of γ-aminobutyric acid-containing composition using non-washed rice bran (skin lees)]
2.5 kg of skin folds generated during the manufacture of BG-free rice and 13.6 L of water were placed in a 30 L jar and mixed well. After adjusting the mixed solution to pH 4.5 with lactic acid, add 1/1000 (based on the weight of rice bran) of commercially available enzyme liquefaction amylase enzyme, proteolytic enzyme agent, fiber degrading enzyme agent, and lipolytic enzyme agent, While stirring, the enzyme treatment was performed by the following temperature raising program. The temperature is raised from 40 ° C. to 55 ° C. within 60 to 90 minutes after the start of stirring, and further reaches 210 ° C. after 210 minutes from the start of stirring and is maintained at 100 ° C. for 10 minutes. The treatment solution was sterilized by autoclave (121 ° C., 15 minutes). After sterilization, after adding an aqueous solution containing 600 g of sterilized MSG separately, inoculate 1 L of Lactobacillus brevis IFO12005 preculture solution (viable cell count: 1.0X10 9 cfu / ml) and stir at 30 ° C (50 rpm )did. After 2 days of culture, all the added MSG was converted to γ-aminobutyric acid. The culture solution was subjected to solid-liquid separation by centrifugation. As a result, 19.2 kg of a supernatant solution having a γ-aminobutyric acid concentration of 16.3 mg / g was obtained. The precipitate was dried to obtain 860 g of a dried product having a γ-aminobutyric acid concentration of 20.0 mg / g.
試験例１（γ-アミノ酪酸含有組成物の飼料特性および飼料価値の評価）
（１）γ-アミノ酪酸含有組成物の飼料特性の評価
製造例１で得られたγ-アミノ酪酸含有組成物を化学分析し、飼料としての特性を評価した。γ-アミノ酪酸含有組成物（固形物）の化学成分を表１に示した。
表１に示すように、γ-アミノ酪酸含有組成物（固形物）は、粗脂肪含量の高さが特徴的であり、粗蛋白含量も比較的高いことが明らかになった。
Test Example 1 (Evaluation of feed characteristics and feed value of γ-aminobutyric acid-containing composition)
(1) Evaluation of feed characteristics of γ-aminobutyric acid-containing composition The γ-aminobutyric acid-containing composition obtained in Production Example 1 was chemically analyzed to evaluate its characteristics as a feed. Table 1 shows the chemical components of the γ-aminobutyric acid-containing composition (solid material).
As shown in Table 1, the γ-aminobutyric acid-containing composition (solid) was characterized by a high crude fat content and a relatively high crude protein content.
（２）γ-アミノ酪酸含有組成物のウシ第一胃内消失率の測定
第一胃カニューレ装着牛を用いて、ナイロンバッグ法により、製造例１で得られたγ-アミノ酪酸含有組成物のウシ第一胃内消失率を測定した。γ-アミノ酪酸含有組成物（固形物）のウシ第一胃内消失率の結果と、大豆粕の第一胃内消失率の結果の比較を図１に示した。
図１に示されるように、製造例１で得られたγ-アミノ酪酸含有組成物（固形物）のウシの第一胃内分解率は比較的緩やかで、20％以上が第一胃内での分解を免れる可能性がみられた。
(2) Measurement of disappearance rate in bovine rumen of γ-aminobutyric acid-containing composition Using a cow with a rumen cannula, the nylon bag method was used to measure the γ-aminobutyric acid-containing composition obtained in Production Example 1. Bovine rumen elimination rate was measured. FIG. 1 shows a comparison between the results of the disappearance rate of bovine rumen in the composition containing γ-aminobutyric acid (solid) and the disappearance rate of rumen in soybean meal.
As shown in FIG. 1, the γ-aminobutyric acid-containing composition (solid matter) obtained in Production Example 1 has a relatively slow rumen degradation rate in cattle, with 20% or more in the rumen. There was a possibility of avoiding disassembly.
（３）γ-アミノ酪酸含有組成物の味パターン
味覚センサーを用いて、製造例１で得られたγ-アミノ酪酸含有組成物の味パターンを一般的な飼料である圧片トウモロコシ、市販の乳牛用の配合飼料と比較して評価した。結果を図２に示した。
図２に示されるように、製造例１で得られたγ-アミノ酪酸含有組成物（固形物）の味は圧片トウモロコシに比べて酸味が強く、甘味が弱い傾向が見られた。
(3) Taste Pattern of γ-Aminobutyric Acid-Containing Composition Using a taste sensor, the taste pattern of the γ-aminobutyric acid-containing composition obtained in Production Example 1 is used as a general feed, pressed corn, and a commercially available dairy cow It was evaluated in comparison with the formula feed. The results are shown in FIG.
As shown in FIG. 2, the taste of the γ-aminobutyric acid-containing composition (solid material) obtained in Production Example 1 showed a strong acidity and a weak sweetness tendency compared to the pressed corn.
（４）γ-アミノ酪酸含有組成物の嗜好性等の評価
ウシ（哺乳子牛）およびブタ（離乳子豚）におけるγ-アミノ酪酸含有組成物の嗜好性等を評価した。哺乳子牛（約１ヶ月齢のホルスタイン種雄牛）における給与量と摂取拒否行動の有無を表２に示した。また、２６頭を一群として飼育される２０日齢の離乳子豚におけるγ-アミノ酪酸含有組成物を配合した飼料給予後30分間のγ-アミノ酪酸含有組成物摂取量を図３に示した。
(4) Evaluation of palatability of γ-aminobutyric acid-containing composition The palatability of the γ-aminobutyric acid-containing composition in cattle (mammals) and pigs (weaning piglets) was evaluated. Table 2 shows the amount of feeding and the presence or absence of intake refusal behavior in suckling calves (about 1 month old Holstein bull). In addition, FIG. 3 shows the intake of the γ-aminobutyric acid-containing composition for 30 minutes after feeding the feed containing the γ-aminobutyric acid-containing composition in a 20-day-old weaned piglet fed as a group of 26 animals.
表２によれば、約１ヶ月齢のホルスタイン種雄牛において、給与９例中の１例において摂取拒否行動が認められた。
また、図３によれば、離乳子豚では、γ-アミノ酪酸含有組成物（固形物）の配合割合が増加することでγ-アミノ酪酸含有組成物（固形物）の摂取量には増加が見られ、本実験条件ではγ-アミノ酪酸含有組成物を配合した飼料給予後30分間で213.7g（１頭あたり平均8.2g）を自発的に摂取した。
According to Table 2, in a Holstein bull of about 1 month of age, intake refusal behavior was observed in 1 out of 9 cases.
Moreover, according to FIG. 3, in the weaned piglet, an increase in the intake of the γ-aminobutyric acid-containing composition (solid matter) increases as the blending ratio of the γ-aminobutyric acid-containing composition (solid matter) increases. As seen, under this experimental condition, 213.7 g (average 8.2 g per animal) was voluntarily ingested for 30 minutes after feeding with the composition containing γ-aminobutyric acid.
試験例２（γ-アミノ酪酸含有組成物を含む飼料の組成および製造方法の検討）
まずγ-アミノ酪酸含有組成物と混合する素材の選定を行った。γ-アミノ酪酸含有組成物は飼料特性の評価により、粗脂肪含量が比較的高く、また、酸味が強く、甘味が弱い傾向が認められたため、γ-アミノ酪酸含有組成物と混合する素材には、粗脂肪含量が低く、甘みを有する事を特徴とし、さらに、飼料として使用可能な安全性を有し、且つ、国内産である素材、或いは、比較的入手が容易で安価な素材が適する。米粉糖化液は国内産の米糠を原料として製造され、甘味があり粗脂肪含量が低く、また、糖蜜も甘味があり、粗脂肪含量が低い。また、トウモロコシ粉末も粗脂肪含量が低く、さらに、試験例１における味覚センサーによる評価により甘味があると評価された。これらの材料は安全性の点で飼料として使用可能であることから、γ-アミノ酪酸含有組成物と混合する素材として選定した。
Test example 2 (examination of composition and production method of feed containing γ-aminobutyric acid-containing composition)
First, a material to be mixed with the γ-aminobutyric acid-containing composition was selected. The composition containing γ-aminobutyric acid was found to have a relatively high crude fat content, a strong acidity, and a tendency to have a weak sweetness as a result of evaluation of feed characteristics. It is characterized by low crude fat content and sweetness. Furthermore, it is safe to use as feed, and is a domestically produced material or a relatively easily available and inexpensive material. Rice flour saccharified liquor is produced using domestically produced rice bran as a raw material and has a sweet and low crude fat content. Molasses also has a sweet and low crude fat content. In addition, the corn powder also had a low crude fat content, and was further evaluated as having a sweet taste by evaluation with a taste sensor in Test Example 1. Since these materials can be used as feed in terms of safety, they were selected as materials to be mixed with the γ-aminobutyric acid-containing composition.
γ-アミノ酪酸含有組成物を含む飼料の組成および製造方法を検討した。γ-アミノ酪酸含有組成物を含む飼料の組成を表３に示した。 The composition and production method of the feed containing the γ-aminobutyric acid-containing composition were studied. Table 3 shows the composition of the feed containing the γ-aminobutyric acid-containing composition.
（１）重量当たり１％以上のγ-アミノ酪酸含有組成物（固形物）と１％以上の米粉糖化液を加え、回転刃を有するブレンダー或いはカッターミキサーを用いて３,０００ｒｐｍ以上、２から５分間撹拌混合した。この撹拌混合操作の結果得られる混合物の状態は粉体であった。
（２）重量当たり１％以上のγ-アミノ酪酸含有組成物（固形物）と１％以上の糖蜜を、回転刃を有するブレンダー或いはカッターミキサーを用いて約３,０００ｒｐｍ、５分間撹拌混合した。この撹拌混合操作の結果得られた混合物は、糖蜜含量が30%(W/W)付近では粉末状であるが、約70%(W/W)以上ではゲル状となり、さらに、糖蜜含量60%(W/W)付近の混合物を70℃で24時間以上通風乾燥することにより、ブロック状に加工することができた。
（３）重量当たり１％以上のγ-アミノ酪酸含有組成物（固形物）と４mm以下に粉砕処理した乾燥トウモロコシ粒を、回転刃を有するブレンダー或いはカッターミキサーを用いて約３,０００ｒｐｍ、５分間撹拌混合した。この混合操作の結果得られる混合物の状態は粉体であった。
(1) Add 1% or more of γ-aminobutyric acid-containing composition (solid matter) and 1% or more of rice saccharified liquid per weight, and use a blender or cutter mixer having a rotating blade to make 3000 rpm or more, 2 to 5 Stir and mix for minutes. The state of the mixture obtained as a result of this stirring and mixing operation was powder.
(2) 1% or more of γ-aminobutyric acid-containing composition (solid) and 1% or more of molasses per weight were stirred and mixed at about 3,000 rpm for 5 minutes using a blender or cutter mixer having a rotary blade. The mixture obtained as a result of this stirring and mixing operation is powdery when the molasses content is around 30% (W / W), but becomes a gel at about 70% (W / W) or more, and further, the molasses content is 60%. The mixture in the vicinity of (W / W) was dried by ventilation at 70 ° C. for 24 hours or more, so that it could be processed into a block shape.
(3) A composition containing γ-aminobutyric acid (solid matter) of 1% or more by weight and dried corn grains crushed to 4 mm or less at about 3,000 rpm for 5 minutes using a blender or cutter mixer having a rotary blade Stir and mix. The state of the mixture obtained as a result of this mixing operation was a powder.
試験例３（γ-アミノ酪酸含有組成物を含む飼料の性能の実証）
γ-アミノ酪酸含有組成物を含む飼料の性能を実証した。
γ-アミノ酪酸含有組成物を含む飼料例、組成、および状態を表４に、黒毛和種成雌牛６頭２反復、及び９頭を用いた一対比較試験による試験飼料の平均嗜好度を表５に、それぞれ示した。また、γ-アミノ酪酸含有組成物（固形物）を含む飼料およびγ-アミノ酪酸含有組成物の化学組成を表６に示す。
Test Example 3 (Demonstration of performance of feed containing γ-aminobutyric acid-containing composition)
The performance of the feed containing the γ-aminobutyric acid containing composition was demonstrated.
Examples of feeds containing γ-aminobutyric acid-containing composition, composition and state are shown in Table 4, and average preference of test feeds in a paired comparative test using 6 Japanese black cows, 2 repeats, and 9 cows is shown in Table 5. Respectively. Table 6 shows the chemical composition of the feed containing the γ-aminobutyric acid-containing composition (solid) and the γ-aminobutyric acid-containing composition.
（１）表４、表５によれば、γ-アミノ酪酸含有組成物（固形物）を含む飼料は、γ-アミノ酪酸含有組成物そのものよりも嗜好性が高いことが示された。 (1) According to Tables 4 and 5, it was shown that the feed containing the γ-aminobutyric acid-containing composition (solid) has higher palatability than the γ-aminobutyric acid-containing composition itself.
（２）表６によれば、γ-アミノ酪酸含有組成物（固形物）を含む飼料はγ-アミノ酪酸含有組成物そのものよりも粗脂肪含量が低く、飼料に適した化学組成であることが示された。 (2) According to Table 6, the feed containing the γ-aminobutyric acid-containing composition (solid) has a lower crude fat content than the γ-aminobutyric acid-containing composition itself, and has a chemical composition suitable for the feed. Indicated.
本発明は畜産分野での飼料として利用することができ、飼料製造企業或いは団体等に直接技術移転が可能である。更に本発明は畜産分野以外の犬、猫などのペット用飼料にも利用できる。 The present invention can be used as feed in the field of livestock, and the technology can be directly transferred to a feed manufacturing company or organization. Furthermore, this invention can be utilized also for the feed for pets, such as dogs and cats other than the livestock field.
Claims (4)
当該γ-アミノ酪酸含有組成物と、トウモロコシ粉末を、攪拌混合することを特徴とする、；γ-アミノ酪酸含有組成物を含む飼料の製造方法。 At least one selected from the group consisting of germinated brown rice bran, rice bran, rice, defatted rice bran, bran, soybean seed coat, red bean seed coat and bamboo red bean seed coat is mixed with water, adjusted to pH 4.6 or less, and at normal pressure Sterilized by heating , added with glutamic acid or a salt thereof, and then inoculated with lactic acid bacteria and cultured to obtain a γ-aminobutyric acid-containing composition;
The γ-aminobutyric acid-containing composition and corn powder are mixed by stirring ; A method for producing a feed containing the γ-aminobutyric acid-containing composition.
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