JP5093678B2 - 微小対象物放出光検出装置 - Google Patents
微小対象物放出光検出装置 Download PDFInfo
- Publication number
- JP5093678B2 JP5093678B2 JP2008158162A JP2008158162A JP5093678B2 JP 5093678 B2 JP5093678 B2 JP 5093678B2 JP 2008158162 A JP2008158162 A JP 2008158162A JP 2008158162 A JP2008158162 A JP 2008158162A JP 5093678 B2 JP5093678 B2 JP 5093678B2
- Authority
- JP
- Japan
- Prior art keywords
- light
- excitation light
- fluorescence
- excitation
- emitted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6478—Special lenses
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
2)暗電流が結晶シリコンに比べて数桁低いため、冷却の必要がなく、小型化に有利である。
3)半導体微細加工技術によるパターン形成が可能であって、容易に検出器アレイが作製できる。
4)プラズマ化学気相成長法により安価なガラスやプラスチック基板上に直接形成できるため、大量生産性に優れ、低コスト化が容易である。
T. Kamei他、"Contact-lens type of micromachined hydrogenated amorphous Si fluorescence detector coupled with microfluidic electrophoresis devices", Appl. Phys. Lett., Vol.89, pp. 114101-1-3(2006)
11 サンプルリザーバ
12 ウエイストリザーバ
13 陰極リザーバ
14 陽極リザーバ
15 注入チャネル
16 分離チャネル
17 非水平表面部材
17f 非水平表面
18 励起光吸収部材
20 半導体光検出素子
30 励起光源
40 蛍光検出部
42 励起光透過用ピンホール
42’盲孔とした励起光透過用ピンホール
50 光学フィルタ
61 蛍光収集用マイクロレンズ
63 反射防止膜
Le 励起光
Lf 蛍光
Claims (4)
- 励起光源の発する励起光の照射を受けて光透過性チップに載持された微小対象物が蛍光
または燐光という形で放出する放出光を蛍光収集用マイクロレンズで収束させて半導体光
検出素子に入射させ、該半導体光検出素子により該放出光を検出させるべくすると共に、
該半導体光検出素子に入射する励起光に起因する反射・散乱光の抑制手段を設けた微小対
象物放出光検出装置であって;
上記励起光の反射・散乱光抑制手段は、上記蛍光収集用マイクロレンズに穿たれた励起
光透過用ピンホールを含み、上記励起光は該励起光透過用ピンホール内を通って上記微小
対象物を照射すること;
を特徴とする微小対象物放出光検出装置。 - 上記励起光の反射・散乱光抑制手段は、さらに上記光透過性チップにあって上記励起光が出射して行く出射面に形成された、励起光とは垂直ではない面である非水平表面を含み、該励起光が該出射面で発生する反射光が斜めにそらされること;
を特徴とする請求項1に記載の微小対象物放出光検出装置。 - 上記励起光の反射・散乱光抑制手段は、さらに上記蛍光収集用マイクロレンズにあって少なくとも上記励起光が入射する部分に設けられた反射防止膜を含むこと;
を特徴とする請求項1に記載の微小対象物放出光検出装置。 - 上記励起光の反射・散乱光抑制手段は、さらに上記光透過性チップにあって少なくとも上記励起光が出射して行く出射面部分に設けられた励起光吸収部材か、励起光反射防止膜を含むこと;
を特徴とする請求項1に記載の微小対象物放出光検出装置。
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008158162A JP5093678B2 (ja) | 2008-06-17 | 2008-06-17 | 微小対象物放出光検出装置 |
US12/486,504 US8008614B2 (en) | 2008-06-17 | 2009-06-17 | Device for detecting emission light of micro-object having means for suppressing reflected and scattered excitation light |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008158162A JP5093678B2 (ja) | 2008-06-17 | 2008-06-17 | 微小対象物放出光検出装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2009300385A JP2009300385A (ja) | 2009-12-24 |
JP5093678B2 true JP5093678B2 (ja) | 2012-12-12 |
Family
ID=41547431
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2008158162A Expired - Fee Related JP5093678B2 (ja) | 2008-06-17 | 2008-06-17 | 微小対象物放出光検出装置 |
Country Status (2)
Country | Link |
---|---|
US (1) | US8008614B2 (ja) |
JP (1) | JP5093678B2 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012015165A2 (ko) * | 2010-07-30 | 2012-02-02 | 나노바이오시스(주) | 광 투과성 열 블록을 포함하는 pcr 장치 |
EP3089937A4 (en) * | 2013-12-31 | 2017-11-22 | Canon U.S. Life Sciences, Inc. | Printed circuit board designs for laminated microfluidic devices |
GB2562428B (en) * | 2016-02-22 | 2020-04-29 | Hitachi High Tech Corp | Light-emitting detection device |
JP7075974B2 (ja) * | 2020-10-07 | 2022-05-26 | 株式会社日立ハイテク | 発光検出装置 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6867420B2 (en) | 2002-06-03 | 2005-03-15 | The Regents Of The University Of California | Solid-state detector and optical system for microchip analyzers |
US7489401B2 (en) | 2004-03-01 | 2009-02-10 | National Institute Of Advanced Industrial Science And Technology | Device for detecting emission light of micro-object |
JP4521517B2 (ja) * | 2004-03-01 | 2010-08-11 | 独立行政法人産業技術総合研究所 | 微小対象物放出光検出装置 |
US7324202B2 (en) * | 2004-12-07 | 2008-01-29 | Novx Systems Inc. | Optical system |
JP4365832B2 (ja) * | 2006-03-07 | 2009-11-18 | 株式会社日立製作所 | 生化学分析用セル、生化学分析用キット及び生化学分析装置 |
JP2008039655A (ja) * | 2006-08-09 | 2008-02-21 | National Institute Of Advanced Industrial & Technology | 微小対象物放出光検出装置 |
-
2008
- 2008-06-17 JP JP2008158162A patent/JP5093678B2/ja not_active Expired - Fee Related
-
2009
- 2009-06-17 US US12/486,504 patent/US8008614B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2009300385A (ja) | 2009-12-24 |
US20100038522A1 (en) | 2010-02-18 |
US8008614B2 (en) | 2011-08-30 |
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