JP5078685B2 - 微生物計数方法 - Google Patents
微生物計数方法 Download PDFInfo
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- JP5078685B2 JP5078685B2 JP2008067563A JP2008067563A JP5078685B2 JP 5078685 B2 JP5078685 B2 JP 5078685B2 JP 2008067563 A JP2008067563 A JP 2008067563A JP 2008067563 A JP2008067563 A JP 2008067563A JP 5078685 B2 JP5078685 B2 JP 5078685B2
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- 241000223935 Cryptosporidium Species 0.000 description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
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- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
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- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
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- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
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- QZKRHPLGUJDVAR-UHFFFAOYSA-K EDTA trisodium salt Chemical compound [Na+].[Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O QZKRHPLGUJDVAR-UHFFFAOYSA-K 0.000 description 1
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- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
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Description
2 希釈液
3 蛍光標識抗体
4 蛍光粒子
10、40 希釈工程
11、41 希釈試料
20、50、70 加温工程
30 希釈試料の蛍光微粒子計測(α値)工程
60 希釈試料の蛍光微粒子計測(β値)工程
65 検量線の作成工程
80 測定試料の蛍光微粒子計測工程
85 検量線から微生物個数の算出工程
Claims (4)
- 試料中に含まれる検出対象微生物を計数する方法であって、
試料中の検出対象微生物の個数が、蛍光微粒子計測器における測定下限値よりも低くなる希釈倍率(φ倍)で、前記試料の一部を希釈して希釈試料を調製する工程と、
前記希釈試料を蛍光微粒子計測器で測定して、希釈試料中に検出対象微生物が存在しない場合の測定値(α値)を得る工程と、
前記希釈試料中に検出対象微生物が存在しない場合の測定値と前記希釈倍率とを乗算したブランク値(α×φ)を算出する工程と、
前記試料の別の一部を蛍光微粒子計測器で測定して、前記試料に含まれる検出対象微生物の個数の測定値(ζ値)を得る工程と、
前記ブランク値(α×φ)を用いて、前記試料に含まれる検出対象微生物の個数の測定値(ζ値)を補正して、前記試料に含まれる検出対象微生物の個数の補正値(δ値)を算出する工程と
を含む微生物計数方法。 - 前記補正値(δ値)の算出工程が、前記試料に含まれる検出対象微生物の測定値(ζ値)から前記ブランク値(α×φ)を減算することで、前記補正値(δ値)を算出する請求項1に記載の微生物計数方法。
- 前記試料の更に別の一部を、前記希釈倍率(φ倍)で希釈して別の希釈試料を調製する工程と、
前記別の希釈試料に、蛍光微粒子計測器が検出対象微生物として計数する蛍光粒子を、既知の個数(γ値)添加した後、蛍光微粒子計測器で測定し、前記蛍光粒子の個数の測定値(β値)を得る工程と
を更に含み、
前記補正値(δ値)の算出工程が、前記ブランク値(α×φ)とともに、前記蛍光粒子の既知の個数(γ値)と、前記蛍光粒子の個数の測定値(β値)と、前記検出対象微生物が存在しない場合の測定値(α値)を用いて、蛍光微粒子計測器の測定値(ζ値)に対する試料中の検出対象微生物の個数(δ値)を示す検量線を作成し、この検量線を用いて行う請求項1に記載の微生物計数方法。 - 前記検量線を作成する際に、前記蛍光粒子の既知の個数(γ値)に対する蛍光微粒子計測器での測定値を、β+α(φ−1)の式により計算する請求項3に記載の微生物計数方法。
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JP5078685B2 true JP5078685B2 (ja) | 2012-11-21 |
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JP5779000B2 (ja) * | 2011-06-07 | 2015-09-16 | メタウォーター株式会社 | 水質測定装置及び水質測定方法 |
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JPH07140148A (ja) * | 1993-11-15 | 1995-06-02 | Canon Inc | 微生物計数方法 |
JPH0815191A (ja) * | 1994-07-05 | 1996-01-19 | Kurita Water Ind Ltd | 懸濁物質濃度計測装置 |
JP2003254960A (ja) * | 2002-03-06 | 2003-09-10 | Fuji Electric Co Ltd | 試料水中の微粒子の個数濃度測定方法および装置 |
JP4004441B2 (ja) * | 2003-07-31 | 2007-11-07 | 株式会社堀場製作所 | 粒径分布測定装置 |
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