JP4982839B2 - 硝子体可視化剤 - Google Patents
硝子体可視化剤 Download PDFInfo
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- JP4982839B2 JP4982839B2 JP2005182412A JP2005182412A JP4982839B2 JP 4982839 B2 JP4982839 B2 JP 4982839B2 JP 2005182412 A JP2005182412 A JP 2005182412A JP 2005182412 A JP2005182412 A JP 2005182412A JP 4982839 B2 JP4982839 B2 JP 4982839B2
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- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
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- A61K49/0076—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the agent being in a particular physical galenical form dispersion, suspension, e.g. particles in a liquid, colloid, emulsion
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
- A61K31/717—Celluloses
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- A61K31/00—Medicinal preparations containing organic active ingredients
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- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/728—Hyaluronic acid
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/74—Synthetic polymeric materials
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
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- A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
- A61K49/0054—Macromolecular compounds, i.e. oligomers, polymers, dendrimers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/006—Biological staining of tissues in vivo, e.g. methylene blue or toluidine blue O administered in the buccal area to detect epithelial cancer cells, dyes used for delineating tissues during surgery
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
- A61P27/12—Ophthalmic agents for cataracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Description
月刊 眼科 2001年6月号(Vol.43 No.6) P817-P821 手術手技 「Triamcinoloneで硝子体を可視化した硝子体手術」坂本 泰二著
硝子体を可視化するため眼内に注入する硝子体可視化剤であって、
正負いずれかの電荷を帯びる官能基を有する高分子物質と、
前記高分子物質を懸濁させる非水溶性かつ非ステロイド性の有色物質と、
を含有することを特徴とする。
<硝子体可視化剤の構造>
硝子体を可視化するにあたり、最低限必要とされる要素としては、硝子体に対する接着性及び有色性である。本実施形態に係る硝子体可視化剤では、これら2つの必須条件の各々を満たす2つの物質を混合することによって実現する。
(1)カルボキシルメチルセルロースNa
ゼラチンの側鎖の官能基を修飾し等電点を4段階に変化させた4種類のゼラチンを用意した(A−D)。それぞれのゼラチンのFITC導入率が同一になるように作成した。
豚眼から摘出した硝子体(ゼリー状)を、tissue lyser を使用して撹拌し粉々(液体)にする。硝子体液1mlを、シャーレに入れて乾燥させる。
FITCで標識したゼラチン水溶液(A−D)を、それぞれ別のシャーレに注入して30秒待つ。ゼラチン水溶液を吸引除去、超純水で洗浄した後、乾燥させる。余分なゼラチンはこの操作で除去され、硝子体に接着したゼラチンのみが残留する。
Proteinase Kにより硝子体を酵素的に分解し、得られたサンプルの蛍光強度を測定し、硝子体に付着したFITCの量を求める。このように、4種類のゼラチンの、硝子体との接着性を定量的に比較した。
実験の結果を図1に示す。ゼラチンAがカルボキシル基(-COOH)を一番多く含み、ゼラチンDがアミノ基(-NH2)を一番多く含む。B,Cはその中間である。AとB、AとD、BとD、CとDの間で有意差を認める(p<0.05)。電荷が中性に近いゼラチンB,Cに比べて、正に強く帯電しているゼラチンD、または、負に強く帯電しているゼラチンAが硝子体とより強く吸着している、と結論できる。これにより、硝子体と高分子の吸着作用に電荷が重要な役割を果たしていることが証明された。
βリン酸3カルシウム。人工骨に用いられる素材で、非水溶性であり生理活性を持たない。
乳酸<HC-(CH3)(OH)(COOH)>を単量体として、エステル結合を繰り返し重合させた高分子物質である。グリシン<H2C-(OH)(COOH)>を単量体に加えることもある。生体内ではゆっくりと加水分解され、二酸化炭素と水に分解される。
直鎖状の(2)が毛玉状に絡まり、球状になった加工物である。直径が数ナノメートルから数百マイクロメートルまで作成が可能である。
硝子体の切除は、まず、網膜と硝子体の接着をはがすことから始まる(人工的後部硝子体剥離)。この人工的後部硝子体剥離の前に、硝子体可視化剤の注入を行なう。硝子体可視化剤の注入は、基本的には一回で十分である。この時点で、眼内は、図2に示す状態になる。図2は、上向きの眼球101の断面図である。ここで、102は、水晶体であり、103は、脈絡膜、104は、網膜、105は、硝子体である。通常、硝子体は、水晶体102下の硝子体腔と呼ばれる空間に満たされたいるが、図2は、器具107により、半分以上が切除された状態を示している。
Claims (6)
- 硝子体を可視化するため眼内に注入する硝子体可視化剤であって、
前記硝子体に対する接着性を有し、カルボキシル基またはアミノ基を有する高分子物質と、
前記高分子物質中に懸濁されており、前記硝子体を可視化する、ポリ乳酸、ポリ乳酸粒子及びセラミックス人工骨の中から選択される有色物質と、
を含有することを特徴とする硝子体可視化剤。 - 前記カルボキシル基またはアミノ基を有する高分子物質は、カルボキシルセルロースナトリウム、ゼラチン及びヒアルロン酸ナトリウムの中から選択される高分子物質であることを特徴とする請求項1に記載の硝子体可視化剤。
- 前記カルボキシル基またはアミノ基を有する高分子物質は、ゼラチン及びヒアルロン酸ナトリウムの中から選択される高分子物質であることを特徴とする請求項1に記載の硝子体可視化剤。
- 前記有色物質の粒子径は、100ナノメートルから10マイクロメートルの範囲であることを特徴とする請求項1に記載の硝子体可視化剤。
- 前記有色物質は、前記硝子体可視化剤中に1重量%から10重量%の範囲で含まれていることを特徴とする請求項1に記載の硝子体可視化剤。
- 更に酵素を含有し、網膜硝子体境界面に作用して硝子体を酵素的に融解させることを特徴とする請求項1に記載の硝子体可視化剤。
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005182412A JP4982839B2 (ja) | 2005-06-22 | 2005-06-22 | 硝子体可視化剤 |
KR1020077030386A KR20080016677A (ko) | 2005-06-22 | 2006-06-16 | 유리체 가시화제 |
MX2007015707A MX2007015707A (es) | 2005-06-22 | 2006-06-16 | Agente para visualizar cuerpo vitreo. |
EP06766822.8A EP1911471A4 (en) | 2005-06-22 | 2006-06-16 | MEDIUM FOR VISUALIZING THE GLASS BODY |
PCT/JP2006/312134 WO2006137341A1 (ja) | 2005-06-22 | 2006-06-16 | 硝子体可視化剤 |
CN2006800223342A CN101203251B (zh) | 2005-06-22 | 2006-06-16 | 玻璃体可视化剂 |
CA002613244A CA2613244A1 (en) | 2005-06-22 | 2006-06-16 | Vitreous body visualization agent |
US11/960,978 US20080147039A1 (en) | 2005-06-22 | 2007-12-20 | Vitreous body visualization agent |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005182412A JP4982839B2 (ja) | 2005-06-22 | 2005-06-22 | 硝子体可視化剤 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007001901A JP2007001901A (ja) | 2007-01-11 |
JP4982839B2 true JP4982839B2 (ja) | 2012-07-25 |
Family
ID=37570371
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Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2005182412A Active JP4982839B2 (ja) | 2005-06-22 | 2005-06-22 | 硝子体可視化剤 |
Country Status (7)
Country | Link |
---|---|
US (1) | US20080147039A1 (ja) |
EP (1) | EP1911471A4 (ja) |
JP (1) | JP4982839B2 (ja) |
KR (1) | KR20080016677A (ja) |
CN (1) | CN101203251B (ja) |
CA (1) | CA2613244A1 (ja) |
WO (1) | WO2006137341A1 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITVR20120051A1 (it) * | 2012-03-20 | 2013-09-21 | Bbs Srl | Composizione colorata per uso in metodi chirurgici oftalmici |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4819617A (en) * | 1986-09-04 | 1989-04-11 | University Of Florida | Viscoelastic material for ophthalmic surgery |
US5461081A (en) * | 1989-09-28 | 1995-10-24 | Alcon Laboratories, Inc. | Topical ophthalmic pharmaceutical vehicles |
ZA912770B (en) * | 1990-04-16 | 1992-01-29 | Bethesda Eye Inst | Enzymatic disinsertion of vitreous body |
EP0963759A1 (en) * | 1998-05-08 | 1999-12-15 | Gerrit Reinold Jacob Melles | The use of a vital dye for facilitating surgical procedures for cataract extraction |
AUPQ155799A0 (en) * | 1999-07-09 | 1999-08-05 | Coroneo, Minas Theodore | Therapeutic methods and uses |
US6395294B1 (en) * | 2000-01-13 | 2002-05-28 | Gholam A. Peyman | Method of visualization of the vitreous during vitrectomy |
EP1132065A1 (en) * | 2000-03-07 | 2001-09-12 | Gerrit Reinold Jacob Melles | Coloured visco-elastic composition |
JP2002136588A (ja) * | 2000-11-01 | 2002-05-14 | Kuraray Co Ltd | 医療用栓材 |
KR20040011426A (ko) * | 2000-11-06 | 2004-02-05 | 알콘, 인코퍼레이티드 | 수술용 비흡출 전이성 점탄물 |
US20030060447A1 (en) * | 2002-04-24 | 2003-03-27 | Mutlu Karakelle | Non-aspirating transitional viscoelastics for use in surgery |
TWI290835B (en) * | 2002-02-22 | 2007-12-11 | Santen Pharmaceutical Co Ltd | Fine particle subconjunctival administration drug delivery system |
US20070148074A1 (en) * | 2003-01-16 | 2007-06-28 | Mostafa Sadoqi | Nanoparticle based stabilization of ir fluorescent dyes |
FR2850282B1 (fr) * | 2003-01-27 | 2007-04-06 | Jerome Asius | Implant injectable a base de ceramique pour le comblement de rides, depressions cutanees et cicatrices, et sa preparation |
CA2567411A1 (en) * | 2004-05-31 | 2005-12-08 | Senju Pharmaceutical Co., Ltd. | Transparent tissue-visualizing preparation |
US7732152B2 (en) * | 2005-11-30 | 2010-06-08 | Wisconsin Alumni Research Foundation | Liquid crystalline substrates for culturing cells |
US20070218007A1 (en) * | 2006-03-17 | 2007-09-20 | Allergan, Inc. | Ophthalmic visualization compositions and methods of using same |
-
2005
- 2005-06-22 JP JP2005182412A patent/JP4982839B2/ja active Active
-
2006
- 2006-06-16 WO PCT/JP2006/312134 patent/WO2006137341A1/ja active Application Filing
- 2006-06-16 KR KR1020077030386A patent/KR20080016677A/ko not_active Application Discontinuation
- 2006-06-16 CN CN2006800223342A patent/CN101203251B/zh not_active Expired - Fee Related
- 2006-06-16 CA CA002613244A patent/CA2613244A1/en not_active Abandoned
- 2006-06-16 EP EP06766822.8A patent/EP1911471A4/en not_active Withdrawn
-
2007
- 2007-12-20 US US11/960,978 patent/US20080147039A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
KR20080016677A (ko) | 2008-02-21 |
US20080147039A1 (en) | 2008-06-19 |
EP1911471A1 (en) | 2008-04-16 |
CN101203251B (zh) | 2012-08-15 |
JP2007001901A (ja) | 2007-01-11 |
EP1911471A4 (en) | 2014-04-23 |
CN101203251A (zh) | 2008-06-18 |
WO2006137341A1 (ja) | 2006-12-28 |
CA2613244A1 (en) | 2006-12-28 |
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