JP4764341B2 - ポリメラーゼ連鎖反応のための装置 - Google Patents
ポリメラーゼ連鎖反応のための装置 Download PDFInfo
- Publication number
- JP4764341B2 JP4764341B2 JP2006532510A JP2006532510A JP4764341B2 JP 4764341 B2 JP4764341 B2 JP 4764341B2 JP 2006532510 A JP2006532510 A JP 2006532510A JP 2006532510 A JP2006532510 A JP 2006532510A JP 4764341 B2 JP4764341 B2 JP 4764341B2
- Authority
- JP
- Japan
- Prior art keywords
- detection device
- chamber
- housing
- heating element
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000003752 polymerase chain reaction Methods 0.000 title description 10
- 238000001514 detection method Methods 0.000 claims description 54
- 238000010438 heat treatment Methods 0.000 claims description 46
- 230000003287 optical effect Effects 0.000 claims description 19
- 239000003124 biologic agent Substances 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 229920006267 polyester film Polymers 0.000 claims description 3
- 229920001721 polyimide Polymers 0.000 claims description 3
- 239000010409 thin film Substances 0.000 claims description 3
- 239000011810 insulating material Substances 0.000 claims description 2
- 229920006230 thermoplastic polyester resin Polymers 0.000 claims description 2
- 238000005530 etching Methods 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 239000000615 nonconductor Substances 0.000 claims 1
- 239000002861 polymer material Substances 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 description 11
- 239000002184 metal Substances 0.000 description 5
- SYJPAKDNFZLSMV-HYXAFXHYSA-N (Z)-2-methylpropanal oxime Chemical compound CC(C)\C=N/O SYJPAKDNFZLSMV-HYXAFXHYSA-N 0.000 description 2
- 241000193738 Bacillus anthracis Species 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 229920002799 BoPET Polymers 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 239000012809 cooling fluid Substances 0.000 description 1
- 239000012777 electrically insulating material Substances 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229920003223 poly(pyromellitimide-1,4-diphenyl ether) Polymers 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 210000002105 tongue Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
- B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/019—Biological contaminants; Fouling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
10 筐体
20 加熱素子
30 光学アパーチャ
40 計器
Claims (32)
- 試料を受容するように構成されたチャンバを収容する筺体と、前記チャンバ内に配置された加熱素子と、前記チャンバの観察を可能にする光学窓とを備えた検出装置であって、前記筺体の一方の面からそれに対向する面へ向かって前記チャンバ中を通過して流体を流動させそれによって前記チャンバ中の前記試料を冷却するように構成された通路が前記筺体に含まれる検出装置。
- 前記筺体が断熱材を含む、請求項1に記載の検出装置。
- 前記筺体が電気絶縁体を含む、請求項1に記載の検出装置。
- 前記筺体がプラスチックを含む、請求項1に記載の検出装置。
- 前記筺体がポリマ材料を含む、請求項1に記載の検出装置。
- 前記筺体が樹脂を含む、請求項1に記載の検出装置。
- 前記樹脂が熱可塑性ポリエステル樹脂を含む、請求項6に記載の検出装置。
- 前記筺体が約1W/m−K未満の熱伝導度を有する材料を含む、請求項1に記載の検出装置。
- 前記筺体の高さが前記筺体の幅より大きく、かつ前記筺体の奥行きより大きい、請求項1に記載の検出装置。
- 前記筺体が、一つに結合されてチャンバを形成する第一部分および第二部分を含む、請求項1に記載の検出装置。
- 前記第一部分および前記第二部分が噛み合って係合するように構成された、請求項10に記載の検出装置。
- 前記第一部分が突起を含み、前記第二部分がアパーチャを含み、前記突起および前記アパーチャが係合して、前記第一部分および前記第二部分が噛み合って係合することができるように構成された、請求項11に記載の検出装置。
- 前記加熱素子が薄膜抵抗体を含む、請求項1に記載の検出装置。
- 前記薄膜抵抗体が、基板上に配置されたエッチング抵抗素子を含む、請求項13に記載の検出装置。
- 前記基板がポリエステル膜を含む、請求項14に記載の検出装置。
- 前記基板がポリイミド膜を含む、請求項14に記載の検出装置。
- 前記加熱素子が可撓性である、請求項1に記載の検出装置。
- 前記加熱素子が第一発熱体および第二発熱体を含む、請求項1に記載の検出装置。
- 前記第一発熱体が前記チャンバの第一壁上に配置され、かつ前記第二発熱体が前記チャンバの前記第一壁に対向する第二壁上に配置された、請求項18に記載の検出装置。
- 前記流体が空気を含む、請求項1に記載の検出装置。
- 前記通路が上下に配置された複数の溝を含む、請求項1に記載の検出装置。
- 前記通路が前記筺体の第一側面から前記筺体の第二側面まで延在する請求項1に記載の検出装置。
- 前記光学窓は、前記検出装置による前記チャンバの光学的監視を可能にするように構成される、請求項1に記載の検出装置。
- 前記光学窓は、前記筺体の壁を貫通して延びる開口を含む、請求項1に記載の検出装置。
- 前記光学窓は、前記加熱素子を貫通して延びる開口を含む、請求項1に記載の検出装置。
- 前記開口が第一開口と、前記第一開口の下に配置された第二開口とを含む、請求項24に記載の検出装置。
- 前記検出装置が試料の蛍光を検出するように構成された、請求項1に記載の検出装置。
- 前記試料が反応管内に収容される、請求項1に記載の検出装置。
- 前記反応管が、前記チャンバに受容されるように構成された、請求項28に記載の検出装置。
- 生物剤を検出するための携帯計器であって、
アレイ状に配設された複数の筺体を備え、
各筺体は、試料を受容するように構成されたチャンバと、前記チャンバ内に配置された加熱素子と、前記チャンバの観察を可能にする光学窓と、該筺体の一方の面からそれに対向する面へ向かって前記チャンバ中を通過して流体を流動させそれによって前記チャンバ中の前記試料を冷却するように構成された通路とを含む、
携帯計器。 - 前記携帯計器が使用者の手の中に保持されるように構成された、請求項29に記載の携帯検出装置。
- 前記携帯計器が六個の筺体を含む、請求項29に記載の携帯検出装置。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US47344703P | 2003-05-28 | 2003-05-28 | |
US60/473,447 | 2003-05-28 | ||
US10/837,745 | 2004-05-04 | ||
US10/837,745 US7170594B2 (en) | 2003-05-28 | 2004-05-04 | Device for polymerase chain reactions |
PCT/US2004/013313 WO2005009617A1 (en) | 2003-05-28 | 2004-05-20 | Device for polymerase chain reactions |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007502430A JP2007502430A (ja) | 2007-02-08 |
JP4764341B2 true JP4764341B2 (ja) | 2011-08-31 |
Family
ID=33555338
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006532510A Expired - Fee Related JP4764341B2 (ja) | 2003-05-28 | 2004-05-20 | ポリメラーゼ連鎖反応のための装置 |
Country Status (6)
Country | Link |
---|---|
US (1) | US7170594B2 (ja) |
EP (1) | EP1631387B1 (ja) |
JP (1) | JP4764341B2 (ja) |
AU (1) | AU2004259633A1 (ja) |
CA (1) | CA2527528A1 (ja) |
WO (1) | WO2005009617A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8778663B2 (en) * | 2007-09-18 | 2014-07-15 | Lawrence Livermore National Security, Llc. | Thermal cycler |
DE102008009920A1 (de) * | 2008-02-15 | 2009-08-20 | Aj Innuscreen Gmbh | Mobiles Gerät für die Nukleinsäureisolierung |
ES2829971T3 (es) | 2008-09-02 | 2021-06-02 | Tautona Group Lp | Hilos de ácido hialurónico y/o derivados de los mismos, métodos para fabricar los mismos y usos de los mismos |
KR102009505B1 (ko) * | 2019-01-17 | 2019-08-12 | 주식회사 엘지화학 | 유전자 증폭 모듈 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03107742A (ja) * | 1989-09-15 | 1991-05-08 | General Atomic Co | 温度制御付き分光測定器用マイクロピペットアダプタ |
JPH11509136A (ja) * | 1995-06-20 | 1999-08-17 | ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニア | 化学反応用シリコン依存形スリーブ・デバイス |
JP2000346805A (ja) * | 1999-06-09 | 2000-12-15 | Shimadzu Corp | 蛍光分光光度計 |
WO2001049416A1 (en) * | 2000-01-04 | 2001-07-12 | The Regents Of The University Of California | Polymerase chain reaction system |
JP2002507145A (ja) * | 1997-02-28 | 2002-03-05 | シーフィード | 熱交換を行ない光学的に質疑される化学反応アセンブリ |
JP2002515602A (ja) * | 1998-05-19 | 2002-05-28 | シーフィード | マルチチャンネル光検出装置 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2543825A (en) * | 1948-04-26 | 1951-03-06 | Phillips Petroleum Co | X-ray diffraction apparatus |
US4443407A (en) * | 1981-04-02 | 1984-04-17 | Instrumentation Laboratory Inc. | Analysis system |
US4848093A (en) * | 1987-08-24 | 1989-07-18 | Quantum Design | Apparatus and method for regulating temperature in a cryogenic test chamber |
US6521181B1 (en) | 1995-06-20 | 2003-02-18 | The Regents Of The University Of Calfornia | Microfabricated electrochemiluminescence cell for chemical reaction detection |
US6524532B1 (en) | 1995-06-20 | 2003-02-25 | The Regents Of The University Of California | Microfabricated sleeve devices for chemical reactions |
US5863502A (en) | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
US6440725B1 (en) | 1997-12-24 | 2002-08-27 | Cepheid | Integrated fluid manipulation cartridge |
US6191599B1 (en) | 1998-10-09 | 2001-02-20 | International Business Machines Corporation | IC device under test temperature control fixture |
US6403037B1 (en) | 2000-02-04 | 2002-06-11 | Cepheid | Reaction vessel and temperature control system |
-
2004
- 2004-05-04 US US10/837,745 patent/US7170594B2/en not_active Expired - Fee Related
- 2004-05-20 JP JP2006532510A patent/JP4764341B2/ja not_active Expired - Fee Related
- 2004-05-20 CA CA002527528A patent/CA2527528A1/en not_active Abandoned
- 2004-05-20 AU AU2004259633A patent/AU2004259633A1/en not_active Abandoned
- 2004-05-20 EP EP04775920.4A patent/EP1631387B1/en not_active Expired - Lifetime
- 2004-05-20 WO PCT/US2004/013313 patent/WO2005009617A1/en active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03107742A (ja) * | 1989-09-15 | 1991-05-08 | General Atomic Co | 温度制御付き分光測定器用マイクロピペットアダプタ |
JPH11509136A (ja) * | 1995-06-20 | 1999-08-17 | ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニア | 化学反応用シリコン依存形スリーブ・デバイス |
JP2002507145A (ja) * | 1997-02-28 | 2002-03-05 | シーフィード | 熱交換を行ない光学的に質疑される化学反応アセンブリ |
JP2002515602A (ja) * | 1998-05-19 | 2002-05-28 | シーフィード | マルチチャンネル光検出装置 |
JP2000346805A (ja) * | 1999-06-09 | 2000-12-15 | Shimadzu Corp | 蛍光分光光度計 |
WO2001049416A1 (en) * | 2000-01-04 | 2001-07-12 | The Regents Of The University Of California | Polymerase chain reaction system |
Also Published As
Publication number | Publication date |
---|---|
AU2004259633A1 (en) | 2005-02-03 |
WO2005009617A1 (en) | 2005-02-03 |
EP1631387B1 (en) | 2013-06-26 |
US7170594B2 (en) | 2007-01-30 |
CA2527528A1 (en) | 2005-02-03 |
JP2007502430A (ja) | 2007-02-08 |
EP1631387A1 (en) | 2006-03-08 |
US20050002024A1 (en) | 2005-01-06 |
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