JP4726170B2 - Mushroom artificial culture medium and mushroom artificial cultivation method using the same - Google Patents
Mushroom artificial culture medium and mushroom artificial cultivation method using the same Download PDFInfo
- Publication number
- JP4726170B2 JP4726170B2 JP2001267087A JP2001267087A JP4726170B2 JP 4726170 B2 JP4726170 B2 JP 4726170B2 JP 2001267087 A JP2001267087 A JP 2001267087A JP 2001267087 A JP2001267087 A JP 2001267087A JP 4726170 B2 JP4726170 B2 JP 4726170B2
- Authority
- JP
- Japan
- Prior art keywords
- culture medium
- mushroom artificial
- sawdust
- mushrooms
- artificial culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【0001】
【発明の属する技術分野】
本発明は、きのこの人工培養基及びそれを用いたきのこの人工栽培方法に関する。
なお、本発明でいう部や%は特に規定のないかぎり質量基準である。
【0002】
【従来の技術とその課題】
従来、きのこの栽培は、くぬぎ、ぶな、及びならなどの原木を利用したほだ木栽培がほとんどであり、そのため気象条件により収穫が左右されることが多い。
また、最近では、ほだ木栽培用の原木の切り出しのための労働力が不足していることなどによって、原木の入手が困難になりつつある。
さらに、ほだ木栽培では栽培期間が長いこと、即ち、種菌の接種からきのこの収穫までに1年半〜2年も要するので、生産コストが相当高くつくのが現状である。
【0003】
近年、えのきたけ、ひらたけ、なめこ、及びしいたけなどは、鋸屑に米糠を配合した人工培養基を用い、瓶又は箱で培養を行う菌床人工栽培方法が確立され、一年を通じて、四季に関係なく安定してこれらのきのこが収穫できるようになっている。
即ち、農家での副業的性格が強く、小規模生産に頼っていた従来のきのこの栽培が、現在では大規模専業生産が可能で、かつ、原料が入手しやすい菌床人工栽培方法に移りつつある。
【0004】
しかしながら、菌床人工栽培においても、きのこを大量に連続栽培するには、いまだ収率も低く、かつ、栽培期間がかなり長いため、その生産コストは安価とは言えず、今後、これら生産性の改善が切望されている。
【0005】
生産性を改善する方法としては、例えば、特定組成を有するアルミノシリケート系化合物や特定組成を有するマグネシウムアルミニウムシリケート系化合物を人工培養基中に含有させる方法が提案されている(特開平03-210126号公報、特開平03-58716号公報)。
【0006】
しかしながら、これらの方法では充分な収率で生産することができていないのが実状である。
【0007】
一方、アラゴナイトとカルサイトを主成分とする貝化石をきのこの人工培養基に含有させることで、きのこの収率を高めることができることが知られている(例えば、高畠幸司、出合忠広、貝化石を利用した食用きのこ栽培、第50回日本木材学会大会、部門R.きのこ、発表番号R4013)。
【0008】
本発明者は、きのこの人工栽培における課題を解消するために、鋭意努力を重ねた結果、特定の人工培養基を用いることによって、きのこを高収率で栽培できることを知見して本発明を完成するに至った。
【0009】
【課題を解決するための手段】
即ち、本発明は、バテライトを含有してなるきのこの人工培養基であり、該人工培養基を用いてなるきのこの人工栽培方法である。
【0010】
【発明の実施の形態】
以下、本発明をさらに詳しく説明する。
【0011】
本発明で使用するバテライトとは、CaCO3で表される炭酸カルシウムの一種である。
炭酸カルシウムは、バテライトのほかに、カルサイトやアラゴナイトと呼ばれる結晶も存在する。これらの炭酸カルシウムはいずれもCaCO3で表される組成を有するが、結晶構造や物理的性質は大きく異なる。
具体的には、バテライトの比重は2.54で、BET比表面積は5〜20m2/g、カルサイトの比重は2.71で、BET比表面積は5m2/g、及びアラゴナイトの比重は2.90であり、同一組成を有する化合物としては非常に異なった値を示す。
また、自然界に存在する炭酸カルシウムは主にカルサイトとアラゴナイトであり、バテライトは通常の条件では生成しないと言われている。
【0012】
本発明で使用する人工培養基とは、通常、鋸屑、ふすま、及びもみがらなどの炭素源と、米糠や大豆粕等の窒素源との混合物、また、この混合物に水を適当量加え、これを瓶又は箱に圧詰めして調製したもの、さらに、これらに、バテライトを添加したものである。
【0013】
炭素源量、窒素源量、及び水量は、その種類や含有水分量等により変化し、一義的に決定されるものではないが、例えば、炭素源として鋸屑を用いる場合、鋸屑は、乾燥状態で培地全乾物量の20〜90%程度の範囲で使用することができるが、この量は窒素源として用いる培地成分によって変動する。例えば、窒素源に米糠を使用する場合、鋸屑は乾物量として培地全乾物量の20〜90%の範囲で使用するが、鋸屑と米糠を重量比で1:1で混合した混合物に水を加えて、水分含水率を55〜70%に調整したものを、瓶又は箱に圧詰めして調製することが好ましい。
【0014】
また、鋸屑としては広葉樹鋸屑あるいは針葉樹鋸屑をそれぞれ単独あるいは混合して使用することも可能である。
【0015】
バテライトの混合割合は、炭素源と窒素源からなる人工培養基100部に対して、1〜30部が好ましく、3〜15部がより好ましい。バテライトの混合割合が前記範囲外では、本発明の効果が充分に得られない場合がある。
【0016】
本発明に係るきのこは、人工栽培できるきのこであれば特に限定されるものではない。その具体例としては、例えば、えのきたけ、ひらたけ、なめこ、ぶなしめじ、及びしいたけなどが挙げられる。
【0017】
本発明では、バテライト、鋸屑等の炭素源、米糠等の窒素源、及び水のほかに、従来より利用されている、きのこの収率を向上させる材料である、アルミノシリケート系化合物、マグネシウムアルミニウムシリケート系化合物、マグネシウムシリケート系化合物、カルシウムシリケート系化合物、マグネシウムアルミネート系化合物、カルシウムアルミネート系化合物、スラグ粉末、エトリンガイト、リン化合物、水酸化カルシウム、カルサイト、及びアラゴナイトなどを本発明の目的を実質的に阻害しない範囲で併用することが可能である。
【0018】
【実施例】
以下、実験例により本発明を詳細に説明する。
【0019】
実験例1
広葉樹鋸屑500g、針葉樹鋸屑500g、及び米糠1,000gをビニール袋に入れ充分に混合して人工培養基を調製した。
調製した人工培養基200gに表1に示すバテライトを添加し、水分含有率を65%に調整して混合物とし、この混合物200gをプラスチック製1リットル広口瓶に圧詰めした。それぞれの瓶の中央に直径1cm程度の穴を開け、打栓後、120℃で90分間殺菌した。
冷却後、ひらたけの鋸屑菌種を植菌し、暗所で温度25℃、相対湿度55%の条件下で30日間培養(菌まわし工程)し、さらに、30日間培養を続けて熟成させた。
次に、栓を外して培養基の上部から約1cm程度菌かきをして菌糸層を除いた後、水道水20mlを添加して充分に吸水させた。
4時間放置後、上部に残った水を取り除いて、温度15℃、相対湿度95%、及び照度20ルクスの条件下で、4日間培養して子実体原基を形成させ、さらに照度を200ルクスに上げて、10日間培養を続け、バテライトの重量比が子実体収量におよぼす影響について検討した。結果を表1に併記する。
なお、比較のために、アラゴナイトやカルサイトを主成分とする貝化石を用いて同様の実験を行った。結果を表1に併記する。
【0020】
<使用材料>
広葉樹鋸屑:ぶな材の鋸屑
針葉樹鋸屑:すぎ材の鋸屑
米糠 :市販品
バテライト:20℃の炭酸ナトリウムの1モル/リットル溶液900ccに、20℃の塩化カルシウムの3モル/リットル溶液10ccを攪拌しながら徐々に加えて混合し、沈殿生成物を生成させ、さらに攪拌しながら10分間熟成した後、固液分離して沈殿生成物を30℃で乾燥して合成。
塩化カルシウム:試薬1級
炭酸ナトリウム:試薬1級
貝化石 :富山県産貝化石、主成分はアラゴナイト、カルサイト、シリカ質
水 :水道水
【0021】
<測定方法>
コントロール対比:バテライト添加の人工培養基の子実体収量(g)/バテライト無添加の人工培養基の子実体収量(g)×100(%)
【0022】
【表1】
【0023】
【発明の効果】
本発明のきのこの人工培養基を用いることによって、きのこの収率を飛躍的に高めることができる。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an artificial culture medium for mushrooms and an artificial cultivation method for mushrooms using the same.
In the present invention, “parts” and “%” are based on mass unless otherwise specified.
[0002]
[Prior art and its problems]
Conventionally, mushroom cultivation is mostly mortar cultivation using raw wood such as kunugi, beech and nara, and therefore the harvest is often influenced by weather conditions.
In addition, recently, it has become difficult to obtain raw wood due to a lack of labor for cutting out raw wood for cultivating wood.
In addition, the cultivation period is long in hodoki cultivation, that is, it takes one and a half years to two years from the inoculation of the inoculum to the harvest of the mushrooms, so that the production cost is considerably high at present.
[0003]
In recent years, enokitake, hiratake, nameko, shiitake, etc. have been established for artificial cultivation of fungus beds, which are cultured in bottles or boxes, using an artificial culture medium in which rice bran is mixed with sawdust. These mushrooms can be harvested stably.
In other words, the traditional mushroom cultivation, which has a strong side-business character at farmers and relies on small-scale production, is now shifting to a fungus bed artificial cultivation method that allows large-scale specialized production and is easy to obtain raw materials. is there.
[0004]
However, even in fungus bed artificial cultivation, in order to continuously cultivate mushrooms in large quantities, the yield is still low and the cultivation period is quite long, so the production cost cannot be said to be low. Improvement is eagerly desired.
[0005]
As a method for improving productivity, for example, a method in which an aluminosilicate compound having a specific composition or a magnesium aluminum silicate compound having a specific composition is contained in an artificial culture medium has been proposed (Japanese Patent Laid-Open No. 03-210126). JP, 03-58716, A).
[0006]
However, the fact is that these methods cannot be produced in a sufficient yield.
[0007]
On the other hand, it is known that the yield of mushrooms can be increased by adding a shell fossil mainly composed of aragonite and calcite to the mushroom artificial culture medium (for example, Koji Takatsuki, Tadahiro Deai, Edible mushroom cultivation, 50th Annual Meeting of the Japan Wood Society, Division R. Mushroom, presentation number R4013).
[0008]
As a result of intensive efforts to solve the problems in artificial cultivation of mushrooms, the present inventor has found that mushrooms can be cultivated in high yield by using a specific artificial culture medium and completes the present invention. It came to.
[0009]
[Means for Solving the Problems]
That is, the present invention is an artificial culture medium for mushrooms containing vaterite, and an artificial cultivation method for mushrooms using the artificial culture medium.
[0010]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in more detail.
[0011]
The vaterite used in the present invention is a kind of calcium carbonate represented by CaCO 3 .
In addition to vaterite, calcium carbonate has crystals called calcite and aragonite. All of these calcium carbonates have a composition represented by CaCO 3 , but their crystal structures and physical properties are greatly different.
Specifically, the specific gravity of vaterite is 2.54, the BET specific surface area is 5-20 m 2 / g, the specific gravity of calcite is 2.71, the BET specific surface area is 5 m 2 / g, and the specific gravity of aragonite is 2.90, the same. The compound having a composition shows very different values.
In addition, calcium carbonate present in nature is mainly calcite and aragonite, and it is said that vaterite does not form under normal conditions.
[0012]
The artificial culture medium used in the present invention is usually a mixture of a carbon source such as sawdust, bran and rice bran and a nitrogen source such as rice bran and soybean meal, and an appropriate amount of water is added to this mixture. What was prepared by crushing into a bottle or a box, and further, vaterite was added to these.
[0013]
The amount of carbon source, the amount of nitrogen source, and the amount of water vary depending on the type and moisture content, and are not uniquely determined.For example, when sawdust is used as the carbon source, the sawdust is in a dry state. Although it can be used in the range of about 20 to 90% of the total dry matter of the medium, this amount varies depending on the medium components used as the nitrogen source. For example, when rice bran is used as a nitrogen source, sawdust is used as a dry matter amount in the range of 20 to 90% of the total dry matter of the medium, but water is added to a mixture of sawdust and rice bran in a weight ratio of 1: 1. Thus, it is preferable to prepare by adjusting the water content of water to 55 to 70% in a bottle or box.
[0014]
Moreover, as sawdust, hardwood sawdust or coniferous sawdust can be used alone or in combination.
[0015]
The mixing ratio of the vaterite is preferably 1 to 30 parts, more preferably 3 to 15 parts with respect to 100 parts of the artificial culture medium composed of a carbon source and a nitrogen source. If the mixing ratio of the vaterite is out of the above range, the effects of the present invention may not be sufficiently obtained.
[0016]
The mushroom according to the present invention is not particularly limited as long as it can be artificially cultivated. Specific examples thereof include enokitake, hiratake, nameko, bean shimeji, shiitake and the like.
[0017]
In the present invention, in addition to a carbon source such as vaterite and sawdust, a nitrogen source such as rice bran, and water, an aluminosilicate compound and magnesium aluminum silicate that are conventionally used materials that improve the yield of mushrooms The object of the present invention is substantially improved in the scope of the present invention, such as magnesium compounds, magnesium silicate compounds, calcium silicate compounds, magnesium aluminate compounds, calcium aluminate compounds, slag powder, ettringite, phosphorus compounds, calcium hydroxide, calcite, and aragonite. It is possible to use in combination as long as it does not hinder.
[0018]
【Example】
Hereinafter, the present invention will be described in detail by experimental examples.
[0019]
Experimental example 1
An artificial culture medium was prepared by placing 500 g of hardwood sawdust, 500 g of coniferous sawdust and 1,000 g of rice bran in a plastic bag and mixing them well.
Vaterite shown in Table 1 was added to 200 g of the prepared artificial culture medium to adjust the water content to 65% to obtain a mixture, and 200 g of this mixture was packed into a plastic 1-liter wide-mouth bottle. A hole with a diameter of about 1 cm was made in the center of each bottle and after sterilization, the bottle was sterilized at 120 ° C for 90 minutes.
After cooling, scallop sawdust species were inoculated, cultured in the dark at a temperature of 25 ° C. and a relative humidity of 55% for 30 days (fungus spinning process), and further cultured for 30 days for aging.
Next, the stopper was removed, and about 1 cm of bacteria was scraped from the top of the culture medium to remove the mycelium layer, and then 20 ml of tap water was added to absorb water sufficiently.
After standing for 4 hours, remove the water remaining on the top and incubate for 4 days under the conditions of temperature 15 ° C, relative humidity 95% and illuminance 20 lux to form a fruiting body primordium, and illuminance is 200 lux. The effect of the weight ratio of the vaterite on the fruit body yield was examined. The results are also shown in Table 1.
For comparison, the same experiment was conducted using a shell fossil mainly composed of aragonite or calcite. The results are also shown in Table 1.
[0020]
<Materials used>
Hardwood sawdust: Sawdust of beech coniferous sawdust: Sawdust of timber: Commercial product vaterite: Stirring 900 cc of 1 mol / liter of sodium carbonate at 20 ° C and 10 cc of 3 mol / liter of calcium chloride at 20 ° C Gradually added and mixed to form a precipitated product, aged for 10 minutes with further stirring, solid-liquid separated and dried at 30 ° C to synthesize.
Calcium chloride: reagent grade 1 sodium carbonate: reagent grade 1 shell fossil: shellfish fossil from Toyama, the main components are aragonite, calcite, siliceous water: tap water
<Measurement method>
Control contrast: Fruit body yield of artificial culture medium with added vaterite (g) / Fruit body yield of artificial culture medium without added vaterite (g) x 100 (%)
[0022]
[Table 1]
[0023]
【The invention's effect】
By using the artificial culture medium for mushrooms of the present invention, the yield of mushrooms can be dramatically increased.
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2001267087A JP4726170B2 (en) | 2001-09-04 | 2001-09-04 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2001267087A JP4726170B2 (en) | 2001-09-04 | 2001-09-04 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2003070355A JP2003070355A (en) | 2003-03-11 |
JP4726170B2 true JP4726170B2 (en) | 2011-07-20 |
Family
ID=19093271
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001267087A Expired - Fee Related JP4726170B2 (en) | 2001-09-04 | 2001-09-04 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP4726170B2 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS55150894A (en) * | 1979-05-15 | 1980-11-25 | Fujijin Shoyu Kk | Method for culturing edible fungi |
JPS6058017A (en) * | 1983-09-09 | 1985-04-04 | バイオテツク株式会社 | Culture of edible mushroom |
JPS61197490A (en) * | 1985-02-26 | 1986-09-01 | 日清製粉株式会社 | Culture medium for mashroom cultivation |
JPS63196210A (en) * | 1987-02-09 | 1988-08-15 | コスモ技研株式会社 | Support agent for culture of mushroom |
JPH01141527A (en) * | 1987-11-27 | 1989-06-02 | Nisshin Flour Milling Co Ltd | Culture base for mushroom cultivation |
-
2001
- 2001-09-04 JP JP2001267087A patent/JP4726170B2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS55150894A (en) * | 1979-05-15 | 1980-11-25 | Fujijin Shoyu Kk | Method for culturing edible fungi |
JPS6058017A (en) * | 1983-09-09 | 1985-04-04 | バイオテツク株式会社 | Culture of edible mushroom |
JPS61197490A (en) * | 1985-02-26 | 1986-09-01 | 日清製粉株式会社 | Culture medium for mashroom cultivation |
JPS63196210A (en) * | 1987-02-09 | 1988-08-15 | コスモ技研株式会社 | Support agent for culture of mushroom |
JPH01141527A (en) * | 1987-11-27 | 1989-06-02 | Nisshin Flour Milling Co Ltd | Culture base for mushroom cultivation |
Also Published As
Publication number | Publication date |
---|---|
JP2003070355A (en) | 2003-03-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP7055025B2 (en) | Additives for mushroom cultivation, artificial culture groups for mushroom cultivation, medium for mushroom cultivation and artificial cultivation method for mushrooms | |
JP4726170B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP4695907B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP3854093B2 (en) | Hatake shimeji fruit body occurrence rate improver | |
JP4520568B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP4570297B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP4578035B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP4726171B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP3485775B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP2766702B2 (en) | Culture medium for mushroom cultivation | |
JP4570296B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP4578036B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JPH11187762A (en) | Artificial culture medium for mushroom and artificial culture for mushroom using the same | |
JP4570295B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP2860959B2 (en) | Mushroom artificial cultivation method | |
JP4578037B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP2829400B2 (en) | Mushroom artificial cultivation method | |
JP3652877B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JPH11243773A (en) | Artificial culture medium for mushroom and artificial culture of mushroom with the same | |
JP3205083B2 (en) | Artificial cultivation method of Hatake shimeji | |
JP4554010B2 (en) | Artificial cultivation method of Maitake | |
CN112273149B (en) | Mixed matrix for cultivating velvet antler mushroom by using hericium erinaceus mushroom residues and preparation method | |
JP4520587B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP4520616B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
JP3512920B2 (en) | Artificial cultivation method of Hatake Shimeji |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20080423 |
|
A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20080423 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080515 |
|
RD02 | Notification of acceptance of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7422 Effective date: 20080515 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080616 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20100624 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100805 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100924 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20110406 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20110411 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 4726170 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140422 Year of fee payment: 3 |
|
LAPS | Cancellation because of no payment of annual fees |