JP4702677B2 - Seed disinfectant - Google Patents
Seed disinfectant Download PDFInfo
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- JP4702677B2 JP4702677B2 JP2003423166A JP2003423166A JP4702677B2 JP 4702677 B2 JP4702677 B2 JP 4702677B2 JP 2003423166 A JP2003423166 A JP 2003423166A JP 2003423166 A JP2003423166 A JP 2003423166A JP 4702677 B2 JP4702677 B2 JP 4702677B2
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- BCVNCJWDDOITBB-UHFFFAOYSA-N s-(dimethylcarbamoylsulfanyl) n,n-dimethylcarbamothioate;methyl n-[1-(butylcarbamoyl)benzimidazol-2-yl]carbamate Chemical compound CN(C)C(=O)SSC(=O)N(C)C.C1=CC=C2N(C(=O)NCCCC)C(NC(=O)OC)=NC2=C1 BCVNCJWDDOITBB-UHFFFAOYSA-N 0.000 description 2
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- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
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Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
この発明は、イネの種子などの種子消毒剤及び種子消毒方法に関するものである。 The present invention relates to a seed disinfectant such as rice seed and a seed disinfecting method.
播種密度が高い水稲の箱育苗では、育苗期に種子伝染による病害が発生しやすく、これらの病害に対する防除を怠ると移植前の苗に著しい被害が生じる。さらに、種子伝染性病害によって罹病した苗を本田に植え込むと、これが本田で伝染源となり病害の発生を増やすことが多い。したがって、水稲の安定生産には種子伝染性病害の防除は、欠くことができない（例えば、非特許文献１参照）。 Box seedlings of paddy rice with a high sowing density tend to cause diseases due to seed transmission during the seedling season, and if these diseases are neglected, the seedlings before transplanting will be significantly damaged. Furthermore, when seedlings affected by seed-borne diseases are planted in Honda, this often becomes a source of infection in Honda and increases the incidence of diseases. Therefore, control of seed infectious diseases is indispensable for the stable production of paddy rice (for example, see Non-Patent Document 1).
これら種子伝染性の病原菌には、幼苗期にイネを犯すだけでなく、本田移植後の稲体に移り葉部、さらには籾を加害するものも多い。イネの種子伝染性病害の病原菌には大別して細菌と糸状菌とがある。細菌性病害は近年増加傾向にあり、発病状況によっては植え付け不能となるなど被害が大きい。細菌性病害の防除は主に薬剤によるが、薬剤防除効果不良事例が各地で見られ、その原因の一つとして薬剤耐性菌の発生がある。糸状菌性病害の種類も多く、なかにはいもち病菌のように水稲栽培で甚大な被害をもたらすものも含まれ、本田防除のほか種子消毒も広く行われている。健苗を確保し、さらに本田移植後収穫期にいたるまでのこれら病害による被害を抑制する必要があり、そのために種子消毒による初期防除は防除法全体の中でも極めて重要な位置を占めている。 Many of these seed-borne pathogens not only commit rice during the seedling stage, but also move to the rice plant after transplanting to Honda and injure leaves and even straw. Bacteria and filamentous fungi are roughly classified into pathogens of rice seed-borne diseases. Bacterial diseases have been on the rise in recent years, and the damage is significant, such as the inability to plant depending on the disease status. Bacterial diseases are mainly controlled by drugs, but there are cases of poor drug control effect in various places, and one of the causes is the generation of drug-resistant bacteria. There are many types of filamentous fungal diseases, including those that cause enormous damage in rice cultivation, such as rice blast fungus. Seed disinfection is also widely performed in addition to Honda control. It is necessary to secure healthy seedlings and to suppress the damage caused by these diseases until the harvest period after transplanting to Honda. For this reason, initial control by seed disinfection occupies an extremely important position in the overall control method.
表１に、わが国における水稲の種子伝染性病害の発生面積と防除面積（農林水産省統計）を示す。表中の苗立枯病とあるのは、苗立枯細菌病とその他の苗に生ずる細菌性の立枯病も含まれている。防除面積としては床土への農薬処理も含まれている。苗立枯病とばか苗病の防除面積は、育苗箱数を移植後の本田面積に換算して示してある。なお、同じ薬剤で複数の病害に有効なものが多く、実際にはいわゆる同時防除が行われているので、ここに示した防除面積は個々の病害に対する防除面積とはいえない場合もある。ここに示した以外の病害については、現状では種子消毒の規模を示す統計が存在せず、ここに示した数字は本田で殺菌剤を散布した面積である。 Table 1 shows the occurrence area and control area of rice seed-borne diseases in Japan (Statistics from the Ministry of Agriculture, Forestry and Fisheries). The term “seedling blight” in the table includes a bacterial blight and a bacterial blight that occurs in other seedlings. The pest control area includes pesticide treatment on floor soil. The control area of seedling blight and idiot seedling disease is shown by converting the number of seedling boxes into the area of Honda after transplanting. Since many of the same drugs are effective for a plurality of diseases, and so-called simultaneous control is actually performed, the control area shown here may not be a control area for individual diseases. For diseases other than those shown here, there are currently no statistics indicating the scale of seed disinfection, and the numbers shown here are areas where fungicides were sprayed in Honda.
このように種子病害としてもみ枯細菌病、苗立枯細菌病、褐条病、いもち病、ごま葉枯病、ばか苗病などがあり、伝染性が高い多種のものによって育苗に被害が生じ、防除面積や発生面積から種子消毒の重要性を理解することができる。 In this way, there are blast blight, seedling blight, brown streak, rice blast, sesame leaf blight, stupid seedling, etc. as seed diseases, and the seedlings are damaged by a variety of highly contagious, The importance of seed disinfection can be understood from the control area and generation area.
ところで、各県の病害虫防除指針など農家への防除指導要項ではこれら病害防除のための種子消毒がかならず記載されていることから、ほとんどの農家は多種類の病害に対して種子消毒を行っていると考えられる。また、種苗生産組合など種子販売にあたってはあらかじめ消毒した水稲種子を配布していることも多いので、種子消毒はほぼ全国的に普及しているものとみてよい。 By the way, in the guidelines for pest control to farmers, such as guidelines for pest control in each prefecture, seed disinfection for the control of these diseases is always described, so most farmers perform seed disinfection for many types of diseases. it is conceivable that. In addition, seed disinfection is often distributed nationwide because seed rice seeds that have been disinfected in advance are distributed in many cases such as seed production associations.
次に、表２に慣行的に使用されている薬剤を示す。 Next, Table 2 shows commonly used drugs.
そこでこの発明は、従来ほど環境に悪い影響を与えることなくイネの種子などを消毒することができる種子消毒剤及び種子消毒方法を提供しようとするものである。 Therefore, the present invention seeks to provide a seed disinfectant and a seed disinfecting method that can disinfect rice seeds and the like without adversely affecting the environment as in the past.
前記課題を解決するためこの発明では次のような技術的手段を講じている。
(１)この発明の種子消毒剤は、木酢液を有効成分とし、植物の種子を浸漬処理するようにしたことを特徴とする。
In order to solve the above problems, the present invention takes the following technical means.
(1) The seed disinfectant according to the present invention is characterized in that wood vinegar is used as an active ingredient and plant seeds are soaked.
この種子消毒剤は天然由来の木酢液を有効成分としているが、例えばイネの種子などを浸漬処理することにより、これを消毒し病害などへの罹病を抑制することができる。また、イネ以外の植物種子へも使用することができる。 This seed disinfectant contains natural wood vinegar as an active ingredient. For example, by immersing rice seeds, the seed disinfectant can be sterilized to prevent diseases. It can also be used for plant seeds other than rice.
すなわち、従来の化学薬品は合成化合物であり自然環境に対しては異物であり種子消毒した廃液をみだりに河川などに廃棄することはできず、所定の回収・分解処置が必要であったが、この発明の種子消毒剤は合成化学薬剤よりもはるかに環境に対して影響を与える可能性が低く、環境に対する影響を最低限度に抑えて種子消毒を行うことができる。 In other words, conventional chemicals are synthetic compounds that are foreign to the natural environment and cannot be disposed of in rivers, etc., with the waste liquid disinfected by seeds. The seed disinfectant of the invention is much less likely to affect the environment than synthetic chemical agents, and seed disinfection can be performed with minimal environmental impact.
(２) 前記木酢液は、木質原料を乾留・静置して得た粗木酢液を蒸留してなる濃度の異なる複数の粗原料を混合して濃度及び成分調整したこととしてもよい。 (2) The wood vinegar solution may be prepared by mixing a plurality of raw materials having different concentrations obtained by distilling a crude wood vinegar solution obtained by dry distillation and standing of a woody material, and adjusting the concentration and components.
このように構成すると、粗木酢液を蒸留精製して得られる個々の木酢液の（各含有木質成分の）濃度は木質原料の性状（天然産品であり幅がある）や炭化方法・木ガスの冷却条件などに左右されるものの、濃度の異なる複数の粗原料を混合して濃度及び成分調整することにより、酢酸と他の木質成分が所定の濃度でバランスよく含有されて安定した品質を有することとなり、植物に対する消毒作用をコンスタントに発揮することができる。すなわち、木酢液の含有木質成分のバランスが悪いと植物に対する有効な消毒作用が期待できない。 Constituting in this way, the concentration of each wood vinegar obtained by distilling and purifying the crude wood vinegar (the content of each wood component) is the nature of the wood material (natural product and width), the carbonization method and wood gas. Although it depends on cooling conditions, etc., by mixing multiple raw materials with different concentrations and adjusting the concentration and ingredients, acetic acid and other wood components are contained in a well-balanced and stable quality. Thus, the disinfecting action on the plant can be constantly exhibited. That is, if the wood component contained in the wood vinegar is poorly balanced, an effective disinfecting action on the plant cannot be expected.
ここで、前記木質原料として樹木、竹、ヤシ、わら、もみがら等を例示することができる。また、前記濃度は含有されている酢酸やグアヤコール、４−メチルグアヤコールの濃度により評価することができる。すなわち、含有量が比較的に多い酢酸、グアヤコール、４−メチルグアヤコールの濃度によりその他全体の含有木質成分の濃度をほぼ比例的に評価することができる。 Here, examples of the woody material include trees, bamboo, palm, straw, rice bran, and the like. Moreover, the said density | concentration can be evaluated with the density | concentration of the acetic acid contained, the guaiacol, and 4-methyl guaiacol. That is, the concentration of other wood components contained can be evaluated almost proportionally based on the concentrations of acetic acid, guaiacol, and 4-methyl guaiacol that are relatively high in content.
(３) この発明の種子消毒方法は、前記種子消毒剤に植物の種子を浸漬処理するようにしたことを特徴とする。 (3) The seed disinfecting method of the present invention is characterized in that plant seeds are immersed in the seed disinfectant.
このように構成することにより、イネの種子などを消毒し、細菌性病害であるイネ苗立枯細菌病やもみ枯細菌病、褐条病、糸状菌性病害であるいもち病、ごま葉枯病、ばか苗病の水稲種子病害などへの罹病を抑制することができる。 By constructing in this way, rice seeds are sterilized, and bacterial seedling blight and rice blast blight, brown streak, filamentous fungal blast and sesame leaf blight are bacterial diseases. It is possible to suppress the morbidity of the seedling disease of paddy rice seedlings.
（４）水稲種子病害を防除する種子消毒方法
（1）前記種子消毒剤として酢酸濃度が約５．５〜６．０％の木酢液を用い、約５〜40倍希釈してイネの種子を浸漬処理し、水稲種子病害を防除するようにしてもよい。
(4) Seed disinfection method for controlling paddy rice seed disease (1) Using a wood vinegar solution of about 5.5 to 6.0% acetic acid as the seed disinfectant, diluting about 5 to 40 times to make rice seeds Immersion treatment may be used to control rice seed disease.
（2）前記種子消毒剤として酢酸濃度が約０．１３７５〜１．２％のものを用い、イネの種子を浸漬処理し、水稲種子病害を防除するようにしてもよい。 (2) The seed disinfectant having an acetic acid concentration of about 0.1375 to 1.2% may be used, and rice seeds may be dipped to control rice seed diseases.
前記（1）や（2）のように構成すると、糸状菌性病害や細菌性病害などの水稲種子病害の防除に関して非常に有効であった。 When configured as in the above (1) and (2), it was very effective in controlling rice seed diseases such as filamentous fungal diseases and bacterial diseases.
特に、糸状菌性病害であるばか苗病、いもち病の水稲種子病害の防除に関して非常に有効であった。また、ばか苗病に対しては、２０倍希釈液（酢酸濃度は０．０２７５〜０．０３０％）・２４時間浸漬処理は非常に防除価が高く、特に好ましいものであった。 In particular, it was very effective in controlling the rice seed disease caused by the fungal diseases such as the broom seedling disease and the rice blast disease. In addition, for idiotic seedlings, a 20-fold diluted solution (acetic acid concentration: 0.0275 to 0.030%) / 24 hour immersion treatment was particularly preferable because of its very high control value.
さらに、細菌性病害であるイネ苗立枯細菌病の水稲種子病害の防除に関しても満足がいく消毒効果が認められた。 Furthermore, a satisfactory disinfecting effect was observed for the control of rice seed disease caused by the bacterial disease of rice seedling.
（５）細菌性病害の水稲種子病害を防除する種子消毒方法
（1）前記種子消毒剤として酢酸濃度が約５．５〜６．０％の木酢液を用い、約10〜20倍希釈してイネの種子を浸漬処理し、細菌性病害を防除するようにしてもよい。
(5) Seed disinfection method for controlling bacterial seed diseases of bacterial diseases (1) Using a wood vinegar solution of about 5.5-6.0% acetic acid as the seed disinfectant, diluting about 10-20 times Rice seeds may be soaked to control bacterial diseases.
（2）前記種子消毒剤として酢酸濃度が約０．２７５〜０．６０％のものを用い、イネの種子を浸漬処理し、細菌性病害を防除するようにしてもよい。 (2) The seed disinfectant having an acetic acid concentration of about 0.275 to 0.60% may be used to immerse rice seeds to control bacterial diseases.
病害によって有効な処理条件に違いがあるが、前記（1）や（2）のように構成すると、細菌性病害であるイネ苗立枯細菌病の水稲種子病害の防除に関して非常に有効であった。またこの場合、浸漬時間が約１時間程度という短時間でも有効であった点で取り扱いに優れ、好ましいものであった。 Effective treatment conditions vary depending on the disease, but when configured as described in (1) and (2) above, it was very effective in controlling rice seed disease caused by the bacterial disease of rice seedlings. . Further, in this case, it was excellent in handling because it was effective even for a short immersion time of about 1 hour, which was preferable.
（６）糸状菌性病害の水稲種子病害を防除する種子消毒方法
（1）前記種子消毒剤として酢酸濃度が約５．５〜６．０％の木酢液を用い、約20倍希釈してイネの種子を約24時間浸漬処理し、糸状菌性病害を防除するようにしてもよい。
(6) Seed disinfection method for controlling rice seed disease of fungal disease (1) Rice vinegar solution diluted with about 20-fold by using wood vinegar solution with an acetic acid concentration of about 5.5-6.0% as the seed disinfectant The seed may be soaked for about 24 hours to control filamentous fungal diseases.
（2）前記種子消毒剤として酢酸濃度が約０．２７５〜０．３０％のものを用い、イネの種子を約24時間浸漬処理し、糸状菌性病害を防除するようにしてもよい。 (2) The seed disinfectant having an acetic acid concentration of about 0.275 to 0.30% may be used, and rice seeds may be soaked for about 24 hours to control filamentous fungal diseases.
病害によって有効な処理条件に違いがあるが、このように構成すると糸状菌性病害であるばか苗病、いもち病、ごま葉枯病の水稲種子病害の防除に関して、共通して有効であった。 Although effective treatment conditions vary depending on the disease, this configuration is effective in common for controlling rice seed diseases such as the fungal disease, banana disease, rice blast disease, and sesame leaf blight.
この発明は上述のような構成であり、次の効果を有する。 The present invention is configured as described above and has the following effects.
天然由来の木酢液を有効成分とし病害などへの罹病を抑制することができるので、従来の化学薬品ほど環境に悪い影響を与えることなくイネの種子などを消毒することができる種子消毒剤及び種子消毒方法を提供することができる。 Seed disinfectant and seeds that can disinfect rice seeds and the like without adversely affecting the environment as much as conventional chemicals, since natural vinegar liquid can be used as an active ingredient to control disease. Disinfection methods can be provided.
以下、この発明の実施の形態を説明する。 Embodiments of the present invention will be described below.
この実施形態の種子消毒剤は木酢液を有効成分とし、植物を一定時間浸漬処理するようにしている。前記植物として、イネの種子などを例示することができる。 The seed disinfectant of this embodiment uses wood vinegar as an active ingredient, and soaks plants for a certain period of time. Examples of the plant include rice seeds.
前記木酢液は、木質原料を乾留・静置して得た粗木酢液を蒸留してなる濃度の異なる複数の粗原料を混合して濃度及び成分調整している。 The wood vinegar liquid is prepared by mixing a plurality of raw materials having different concentrations obtained by distilling a crude wood vinegar liquid obtained by dry distillation and standing of a woody raw material, and adjusting the concentration and components.
具体的には、次のようにして製造した。すなわち木材を乾留して発生した木ガスを冷却すると、液状物が生ずる。この液状物を静置すると分離し、上層から粗木酢液が下層から木タールが得られる。前記粗木酢液を蒸留し、有害物質を除いて成る濃度の異なる粗原料を複数用意する。蒸留してできた粗原料として、濃度が濃淡色々なものができた。蒸留を何回か繰り返し、濃いグレードの粗原料を作っておいてもよい。 Specifically, it was produced as follows. That is, when wood gas generated by dry distillation of wood is cooled, a liquid material is produced. When this liquid is left standing, it separates, and crude wood vinegar is obtained from the upper layer and wood tar is obtained from the lower layer. The crude wood vinegar is distilled to prepare a plurality of crude materials having different concentrations, excluding harmful substances. As raw materials obtained by distillation, various raw materials were produced. Distillation may be repeated several times to produce a dark grade crude material.
そして前記粗原料を混合ブレンドして、酢酸濃度が約４〜１０％の木酢液とするようにしている。例えば酢酸等の濃度（ガスクロマトグラフィーで測定）が低めの粗原料には、前記酢酸の濃度が濃ゆめの粗原料をブレンドして前記の濃度となるようにする。 And the said raw material is mixed and blended so that the acetic acid concentration may be about 4 to 10%. For example, a crude material having a low concentration of acetic acid or the like (measured by gas chromatography) is blended with a crude material having a low concentration of acetic acid so as to have the above-mentioned concentration.
ここで、前記濃度は含有されている酢酸の他にグアヤコール、４−メチルグアヤコールの濃度により評価することができる。すなわち、含有量が比較的に多い酢酸、グアヤコール、４−メチルグアヤコールの濃度によりその他全体の含有木質成分の濃度をほぼ比例的に評価することができる。 Here, the said density | concentration can be evaluated with the density | concentration of guaiacol and 4-methyl guaiacol other than the acetic acid contained. That is, the concentration of other wood components contained can be evaluated almost proportionally based on the concentrations of acetic acid, guaiacol, and 4-methyl guaiacol that are relatively high in content.
次に、この実施形態の種子消毒剤の使用状態を説明する。 Next, the use state of the seed disinfectant of this embodiment will be described.
この種子消毒剤は天然由来の木酢液を有効成分としているが、例えばイネの種子などの植物を一定時間浸漬処理することにより、これを消毒しイネ苗立枯細菌病などへの罹病を抑制することができ、イネ等の種子などの植物を環境に優しく消毒することができるという利点がある。 This seed disinfectant contains natural wood vinegar as an active ingredient. For example, by immersing plants such as rice seeds for a certain period of time, the seed disinfectant is sterilized to suppress morbidity of rice seedling bacterial diseases. And has an advantage that plants such as rice can be sterilized in an environmentally friendly manner.
またこの種子消毒方法は、前記種子消毒剤に植物を一定時間浸漬処理するようにしており、イネの種子などの植物を消毒しイネ苗立枯細菌病などへの罹病を抑制することができるという利点がある。 In addition, this seed disinfection method is designed to immerse plants in the seed disinfectant for a certain period of time, and disinfect plants such as rice seeds and suppress morbidity such as rice seedling bacteriopathies. There are advantages.
ところで前記木酢液は、木質原料を乾留・静置して得た粗木酢液を蒸留してなる濃度の異なる複数の粗原料を混合して濃度及び成分調整しており、粗木酢液を蒸留精製して得られる個々の木酢液の（各含有木質成分の）濃度は木質原料の性状（天然産品であり幅がある）や炭化方法・木ガスの冷却条件などに左右されるものの、濃度の異なる複数の粗原料を混合して濃度及び成分調整することにより、酢酸と他の木質成分が所定の濃度でバランスよく含有されて安定した品質を有することとなり、植物に対する消毒作用をコンスタントに発揮することができるという利点がある。すなわち、木酢液の含有木質成分のバランスが悪いと植物に対する有効な消毒作用が期待できない。 By the way, the above-mentioned wood vinegar liquid is prepared by distilling crude wood vinegar liquid obtained by dry distillation and standing, and adjusting the concentration and ingredients by mixing multiple raw materials with different concentrations. Concentrations of individual wood vinegar liquids (each of the wood components contained in the product) differ depending on the nature of the wood raw material (natural product and width), carbonization method, wood gas cooling conditions, etc. By mixing multiple raw materials and adjusting the concentration and components, acetic acid and other wood components are contained in a balanced manner at a predetermined concentration and have a stable quality, and constantly exhibit a disinfecting action on plants. There is an advantage that can be. That is, if the wood component contained in the wood vinegar is poorly balanced, an effective disinfecting action on the plant cannot be expected.
また一定品質の木酢液（種子消毒剤）が製造できるので、植物に対する消毒効果が製造ロットが相違しても変わらないという利点がある。 Further, since a certain quality of wood vinegar (seed disinfectant) can be produced, there is an advantage that the disinfection effect on the plant does not change even if the production lot is different.
次に、この発明の構成をより具体的に説明する。 Next, the configuration of the present invention will be described more specifically.
種子消毒剤を、次のようにして製造した。すなわち、木材を乾留して発生したガスを冷却すると、液状物が生ずる。この液状物を、第１の釜（ステンレス製蒸留釜）により液温１００℃以下で減圧蒸留を行った。この粗蒸留液を更に１０日以上静置する事により、軽質油層、水層、沈降タール層の三層に分離させ、酢酸が主成分の水層だけを粗木酢液として抜き出した。 A seed disinfectant was produced as follows. That is, when the gas generated by dry distillation of wood is cooled, a liquid material is produced. This liquid was subjected to vacuum distillation at a liquid temperature of 100 ° C. or lower using a first kettle (stainless steel distillation kettle). The crude distillate was further allowed to stand for 10 days or longer to separate the light oil layer, the aqueous layer and the precipitated tar layer into three layers, and only the aqueous layer containing acetic acid as a main component was extracted as a crude wood vinegar solution.
次に、前記粗木酢液（水層部分）を第２の釜により液温約１００℃で常圧蒸留を行った。この操作では粗木酢液を蒸留釜に入れて初留分を除去した。この蒸留により、メタノールやアセトン、アルデヒド類などの低沸点物を取り除き、これら低沸点物と共沸する可能性がある有害物質（ベンゾ(a) ピレンなど）を取り除いた。 Next, the crude wood vinegar liquid (aqueous layer portion) was subjected to atmospheric distillation at a liquid temperature of about 100 ° C. in a second kettle. In this operation, the crude wood vinegar was placed in a distillation kettle to remove the first fraction. This distillation removed low-boiling substances such as methanol, acetone, and aldehydes, and harmful substances (such as benzo (a) pyrene) that could azeotrope with these low-boiling substances.
第２の蒸留釜に入れた粗木酢液の初留分が除去され、さらに半量程度が蒸留されると蒸留を停止し、釜に残っている半量程度の粗木酢液は第３の釜へ移した。また、蒸留された半量程度は第２の釜に戻して再度蒸留した。これにより、第２の釜から低沸点物及びこれと共沸する可能性があるベンゾ(a) ピレン等をさらに念を入れて確実に取り除いた粗原料を得た。 When the first fraction of the crude wood vinegar liquid in the second distillation kettle is removed and about half of it is distilled, the distillation is stopped, and about half of the crude wood vinegar remaining in the kettle is transferred to the third kettle. did. Further, about half of the distilled amount was returned to the second kettle and distilled again. As a result, a low-boiling substance and benzo (a) pyrene that may azeotrope with the second boiling pot were obtained from the second kettle, and a crude raw material was reliably removed.
低沸点物を取り除いた後に第２の釜に残っていた半量程度を第３の釜により液温約１００〜１２０℃で常圧蒸留して、木酢液の主成分である酢酸を含む留分を効率的に回収した高純度蒸留精製木酢液を粗原料として得た。 About half of the amount remaining in the second kettle after removing the low boilers was distilled at atmospheric pressure in the third kettle at a liquid temperature of about 100 to 120 ° C. to obtain a fraction containing acetic acid as the main component of the wood vinegar liquor. A high-purity distilled and purified wood vinegar solution efficiently recovered was obtained as a crude material.
このような方法で、濃度が色々な粗原料を複数用意した。これらの複数の粗原料を、木酢液１００ｍｌに含まれる重量が以下のような成分濃度となるように混合ブレンドした。 By such a method, a plurality of raw materials having various concentrations were prepared. A plurality of these raw materials were mixed and blended so that the weight contained in 100 ml of the wood vinegar liquid would have the following component concentrations.
酢酸５９１５．１ｍｇ、アセトール９２５．４ｍｇ、プロピオン酸５１２．３ｍｇ、ｎ−酪酸１３７．８ｍｇ、フルフラール１４４．１ｍｇ、フルフリルアルコール３４．４ｍｇ、フェノール８７．７ｍｇ、シクロテン６２．７ｍｇ、ｏ−クレゾール１８．１ｍｇ、ｍ−，ｐ−クレゾール３２．６ｍｇ、グアヤコール２２５．５ｍｇ、４−メチルグアヤコール７０．５ｍｇ、４−エチルグアヤコール０．００ｍｇ、その他成分１１１４．８ｍｇ、水分９２１１８．９ｍｇ（以上、木酢液１００ｍｌ中に含まれる重量）。なお、前記水分の測定はカールフィッシャー法によった。 Acetic acid 5915.1 mg, acetol 925.4 mg, propionic acid 512.3 mg, n-butyric acid 137.8 mg, furfural 143.4 mg, furfuryl alcohol 34.4 mg, phenol 87.7 mg, cycloten 62.7 mg, o-cresol 18. 1 mg, m-, p-cresol 32.6 mg, guaiacol 225.5 mg, 4-methyl guaiacol 70.5 mg, 4-ethyl guaiacol 0.00 mg, other components 1114.8 mg, moisture 92118.9 mg (above, in 100 ml of wood vinegar solution In weight). The moisture was measured by the Karl Fischer method.
こうして酢酸濃度が５．５〜６．０％の木酢液を得、この木酢液を有効成分とする種子消毒剤を得た。 Thus, a wood vinegar solution having an acetic acid concentration of 5.5 to 6.0% was obtained, and a seed disinfectant containing this wood vinegar solution as an active ingredient was obtained.
前記実施形態で得た種子消毒剤（酢酸濃度が５．５〜６．０％）を用い、イネ苗立枯細菌病に対する種子消毒効果試験を行った。 Using the seed disinfectant (acetic acid concentration of 5.5 to 6.0%) obtained in the above embodiment, a seed disinfection effect test against rice seedling bacterial disease was performed.
水稲（品種：日本晴）の健全籾に苗立枯細菌病の病原細菌汚染籾を４％の割合（1区あたり健全籾２８８粒、汚染籾１２粒）で混入した種子試料を、その容量１に対して容量２の種子消毒剤の希釈液に浸漬し、液温１５℃に保って所定時間保存して薬剤処理を行った。 A seed sample containing 4% of pathogenic bacteria contaminated with seedling bacterial disease (288 healthy seeds per ward and 12 contaminated straws per ward) in healthy rice of rice (variety: Nihonbare) On the other hand, it was immersed in a dilute solution of a seed disinfectant having a volume of 2, and kept at a liquid temperature of 15 ° C. and stored for a predetermined time for chemical treatment.
処理後の種子は薬剤処理開始から６日後まで１５℃の真水中に保った後水を切り、２８℃に1日間保って催芽させ、育苗箱中の粒状培土に播種した。播種8日後に、発芽籾数、健全苗数、ならびに罹病苗数を罹病程度を白化と枯死に分けて計数した。 The seeds after the treatment were kept in fresh water at 15 ° C. until 6 days after the start of the chemical treatment, then drained, and kept at 28 ° C. for 1 day for germination, and sown in granular soil in a seedling box. Eight days after sowing, the number of germination pods, the number of healthy seedlings, and the number of diseased seedlings were counted by dividing the disease severity into whitening and withering.
試験は播種籾1区３００粒とし３反復で行った。罹病苗率とともに、罹病程度指数を、０（健全）、３（白化）、および５（枯死）として以下の式により炉病度と防除価を算出した。 The test was repeated 3 times with 300 seeds per seedling. Along with the diseased seedling rate, the disease severity index and 0 (healthy), 3 (whitening), and 5 (dead) were used to calculate the furnace morbidity and control value according to the following equations.
試験対照薬剤としては、本病害防除のための種子消毒に汎用されている市販品オキソリニック酸２０％水和剤(化学農薬)を供試した。なお、無処理対照区として汚染籾混入区（無処理区I）および健全籾のみを播種した区（無処理区II）を設けた。
罹病苗率(％)＝（罹病苗数／調査苗数）×１００
罹病度＝〔｛（白化苗数×３）＋（枯死苗数×５）｝／調査苗数×５〕×１００
防除価＝（処理区の罹病度／無処理区Iの罹病度）×１００
前記計算式から算出した試験結果を、表３に示す。
As a test control drug, a commercially available oxolinic acid 20% wettable powder (chemical agrochemical) widely used for seed disinfection for controlling this disease was used. In addition, as a non-treated control group, a contaminated soot mixed zone (untreated zone I) and a zone where only healthy straws were seeded (untreated zone II) were provided.
Diseased seedling rate (%) = (number of diseased seedlings / number of surveyed seedlings) × 100
Disease level = [{(number of bleached seedlings × 3) + (number of dead seedlings × 5)} / number of surveyed seedlings × 5] × 100
Control value = (morbidity of treated area / morbidity of untreated area I) × 100
Table 3 shows the test results calculated from the above formula.
かに罹病苗率（１．４％）および罹病度（０．８）ともに低く、苗立枯細菌病に対する種子消毒効果が極めて高かった（防除価９８．９）。また、種子消毒剤の２０倍希釈液（酢酸濃度は０．２７５〜０．３０％）・1時間処理区でも対照薬剤であるオキソリニック酸２０％水和剤（防除価６９．９）よりも消毒効果が高かった（防除価８６．１）。
前記試験結果から本種子消毒剤の１０倍希釈液（酢酸濃度は０．５５〜０．６０％）及び２０倍希釈液（酢酸濃度は０．２７５〜０．３０％）の1時間種子浸漬処理による苗立枯細菌病防除は非常に実用性が高いものと判断できる。また、種子消毒剤の２００倍希釈液（酢酸濃度は０．０２７５〜０．０３０％）・６日間処理区では防除価が５３．８と、対照薬剤（防除価６９．９）に迫る消毒効果が認められた。 From the test results, the seed disinfectant was treated with a 10-fold diluted solution (acetic acid concentration: 0.55 to 0.60%) and a 20-fold diluted solution (acetic acid concentration: 0.275 to 0.30%) for 1 hour. It can be judged that the control of the seedling bacterial disease by means of is very practical. In addition, a 200-fold diluted solution of seed disinfectant (acetic acid concentration is 0.0275 to 0.030%). In the 6-day treatment section, the control value is 53.8, and the disinfecting effect is close to the control drug (control value 69.9). Was recognized.
イネばか苗病に対する種子消毒効果を、防除効果試験として一般的に行われている苗箱試験で実施した。水稲苗はそれ自体が商品として流通するため、ばか苗病は本病菌による従長苗の発生が１箱当たりたとえ僅かな本数であっても商品価値を著しく低下させるとして、水稲種子消毒試験の中で最も高い効果を要求される病害である。 The seed disinfection effect against rice sapling seedling disease was carried out in a seedling box test generally performed as a control effect test. Since paddy rice seedlings circulate as commodities themselves, stupid seedlings are considered in rice seed disinfection tests because the product value is markedly reduced even if the number of sprout seedlings caused by this fungus is small per box. It is a disease that requires the highest effect.
種子消毒剤は、上記実施形態の酢酸濃度が５．５〜６．０％の木酢液を有効成分とするものである。供試汚染種子の品種は、ヒノヒカリ（開花期接種籾）である。１反復につき汚染籾１００粒を供試し、３反復で実施した。
所定濃度に希釈（５倍、１０倍、２０倍、４０倍）した種子消毒剤に、汚染籾を浸漬処理した。１０分間、３０分間及び１時間の短時間浸漬処理は、処理後、種子消毒剤から蒸留水に水交換した。２４時間浸漬は、浸漬開始２４時間後に種子消毒剤を蒸留水に交換した。無処理区は、蒸留水でそのまま浸種した。対照は、ヘルシードＴ水和剤である。全ての処理区は１５℃の定温器内で翌日まで管理した。
The seed disinfectant contains wood vinegar solution having an acetic acid concentration of 5.5 to 6.0% in the above embodiment as an active ingredient. The varieties of the contaminated seeds used for the test are Hinohikari (flowering period inoculum). 100 grains of contaminated koji were tested per repetition and were performed in 3 repetitions.
The contaminated soot was immersed in a seed disinfectant diluted to a predetermined concentration (5 times, 10 times, 20 times, 40 times). In the short immersion treatment for 10 minutes, 30 minutes, and 1 hour, the water was exchanged from the seed disinfectant to distilled water after the treatment. In the 24-hour immersion, the seed disinfectant was replaced with distilled water 24 hours after the start of immersion. The untreated section was immersed as it was with distilled water. The control is Hellseed T wettable powder. All treatment sections were managed in a 15 ° C. incubator until the next day.
浸漬処理を終えた種子は、通常の浸種の工程を省略して播種した。正規の育苗箱の十二分の一の大きさのプラスチックケースに、水稲用育苗培土を充填して播種した。播種後４日間は暗黒下２８℃で出芽を促し、その後はガラス室内で通常の育苗管理を行った。そして、播種２３日後に各処理区全ての苗を健全苗、従長苗、腐敗枯死苗に選別し、従長苗と腐敗枯死苗の合計を発病苗数とし、調査苗数に対する発病苗率を求めた。また、不発芽籾数を数え、播種籾数に対する不発芽籾率を算出した。表４に、試験結果を示す。 The seeds that had been soaked were sown by omitting the normal soaking process. A plastic case that is one-twelfth the size of a regular nursery box was filled with seedling culture soil for paddy rice and sown. For 4 days after sowing, germination was promoted at 28 ° C. in the dark, and thereafter normal seedling management was performed in a glass chamber. Then, 23 days after sowing, all seedlings in each treatment area are selected as healthy seedlings, subordinate seedlings, and decayed and killed seedlings. Asked. In addition, the number of non-germinated pods was counted, and the ratio of non-germinated buds to the number of seed pods was calculated. Table 4 shows the test results.
いもち病の汚染水稲種子に対する種子消毒効果を、ろ紙湿室法により検討した。 The seed disinfection effect on rice seed contaminated with rice blast was examined by the filter paper wet chamber method.
種子消毒剤は、上記実施形態の酢酸濃度が５．５〜６．０％の木酢液を有効成分とするものである。供試汚染種子の品種は、ナツミノリ（自然汚染種子）である。１反復につき汚染籾１００粒（２５粒×４シャーレ）を供試し、３反復で実施した。
所定濃度に希釈（５倍、１０倍、２０倍、４０倍）した種子消毒剤に、汚染籾を浸漬処理した。１０分間、３０分間及び１時間の短時間浸漬処理は、処理後、種子消毒剤から蒸留水に水交換した。２４時間浸漬は、浸漬開始２４時間後に種子消毒剤を蒸留水に交換した。無処理区は、蒸留水でそのまま浸種した。対照は、ヘルシード水和剤である。全ての処理区は１５℃の定温器内で翌日まで管理した。
The seed disinfectant contains wood vinegar solution having an acetic acid concentration of 5.5 to 6.0% in the above embodiment as an active ingredient. The test contaminated seed varieties are nutminoli (naturally contaminated seeds). 100 grains (25 grains × 4 dishes) of contaminated koji were used for one repetition, and the experiment was performed three times.
The contaminated soot was immersed in a seed disinfectant diluted to a predetermined concentration (5 times, 10 times, 20 times, 40 times). In the short immersion treatment for 10 minutes, 30 minutes, and 1 hour, the water was exchanged from the seed disinfectant to distilled water after the treatment. In the 24-hour immersion, the seed disinfectant was replaced with distilled water 24 hours after the start of immersion. The untreated section was immersed as it was with distilled water. The control is a healthy seed wettable powder. All treatment sections were managed in a 15 ° C. incubator until the next day.
プラスチックシャーレ（径９ｃｍ、深さ２ｃｍ）にろ紙３枚を重ねて敷き、滅菌水を注いでろ紙に水を十分含ませた後、余分の水を捨てた。前記処理を行った種籾を水切り後、１シャーレに２５粒づつ置床し、２５℃、ＢＬＢランプ照射（１２時間／日）で３日間インキュベートした。各処理区全ての籾について、実体顕微鏡下でいもち病菌分生胞子の形成の有無を調査し、胞子形成籾率を算出した。表５に、試験結果を示す。 Three sheets of filter paper were piled on a plastic petri dish (diameter 9 cm, depth 2 cm), sterilized water was poured into the filter paper to sufficiently contain water, and then excess water was discarded. The seed pods subjected to the above treatment were drained and then placed in a 25 petri dish in a petri dish and incubated at 25 ° C. with BLB lamp irradiation (12 hours / day) for 3 days. With respect to all the cocoons in each treatment section, the presence or absence of blast fungus conidia was examined under a stereomicroscope, and the spore formation rate was calculated. Table 5 shows the test results.
ごま葉枯病汚染水稲種子に対する種子消毒効果を、凍結ろ紙湿室法により検討した。 The seed disinfection effect on rice seeds contaminated with sesame leaf blight was examined by the frozen filter paper wet chamber method.
種子消毒剤は、上記実施形態の酢酸濃度が５．５〜６．０％の木酢液を有効成分とするものである。供試汚染種子の品種は、コシヒカリ（自然汚染種子）である。１反復につき汚染籾１００粒（２５粒×４シャーレ）を供試し、３反復で実施した。
所定濃度に希釈（５倍、１０倍、２０倍、４０倍）した種子消毒剤に、汚染籾を浸漬処理した。１０分間、３０分間及び１時間の短時間浸漬処理は、処理後、種子消毒剤から蒸留水に水交換した。２４時間浸漬は、浸漬開始２４時間後に種子消毒剤を蒸留水に交換した。無処理区は、蒸留水でそのまま浸種した。対照は、ベンレートＴ水和剤２０である。全ての処理区は１５℃の定温器内で翌日まで管理した。
The seed disinfectant contains wood vinegar solution having an acetic acid concentration of 5.5 to 6.0% in the above embodiment as an active ingredient. The varieties of the test contaminated seeds are Koshihikari (naturally contaminated seeds). 100 grains (25 grains × 4 dishes) of contaminated koji were used for one repetition, and the experiment was performed three times.
The contaminated soot was immersed in a seed disinfectant diluted to a predetermined concentration (5 times, 10 times, 20 times, 40 times). In the short immersion treatment for 10 minutes, 30 minutes, and 1 hour, the water was exchanged from the seed disinfectant to distilled water after the treatment. In the 24-hour immersion, the seed disinfectant was replaced with distilled water 24 hours after the start of immersion. The untreated section was immersed as it was with distilled water. The control is Benlate T wettable powder 20. All treatment sections were managed in a 15 ° C. incubator until the next day.
プラスチックシャーレ（径９ｃｍ、深さ２ｃｍ）にろ紙３枚を重ねて敷き、滅菌水を注いでろ紙に水を十分含ませた後、余分の水を捨てた。前記処理を行った種籾を水切り後、１シャーレに２５粒づつ置床し、２５℃、ＢＬＢランプ照射（１２時間／日）で１日半の間インキュベートした。その後、−２０℃で１２時間凍結処理し、再度２日間ＢＬＢ照射した。各処理区全ての籾について、実体顕微鏡下でごま葉枯病菌分生胞子の形成の有無を調査し、胞子形成籾率を算出した。表６に、試験結果を示す。 Three sheets of filter paper were piled on a plastic petri dish (diameter 9 cm, depth 2 cm), sterilized water was poured into the filter paper to sufficiently contain water, and then excess water was discarded. The seed pods subjected to the above treatment were drained, placed in a petri dish by 25 grains, and incubated at 25 ° C. with BLB lamp irradiation (12 hours / day) for one and a half days. Thereafter, it was frozen at −20 ° C. for 12 hours and again irradiated with BLB for 2 days. The presence or absence of formation of sesame leaf blight fungus conidia was examined under a stereomicroscope in all treatment areas, and the spore formation rate was calculated. Table 6 shows the test results.
（まとめ）
以上、実施例１〜４の各試験結果（苗立枯細菌病に対する防除価、ばか苗病に対する防除価、いもち病に対する胞子形成もみ率、ごま葉枯病に対する胞子形成阻止率）を、表７にまとめた。
(Summary)
Table 7 shows the test results of Examples 1 to 4 (controlling value against seedling bacterial disease, control value against banana seedling disease, spore formation rate against blast, and spore formation inhibition rate against sesame leaf blight). Summarized in
（１）苗立枯細菌病に対する防除価
表中No3では種子消毒剤について約10倍希釈（濃度）として約１時間浸漬処理（浸漬時間）したものの防除価は98.9であり、No4では処理の種子消毒剤について約20倍希釈として約１時間浸漬処理したものの防除価は86.1であり、非常に有効であった。また、約200倍希釈として約６日間浸漬処理したものの防除価も53.8と、十分な消毒効果が認められた。
(1) Control value against bacterial wilt of seedlings In No3 in the table, the seed disinfectant was about 10 times diluted (concentration) soaked for about 1 hour (immersion time), and the control value was 98.9. The antiseptic was diluted about 20 times and immersed for about 1 hour, and its control value was 86.1, which was very effective. In addition, the antiseptic value of 53.8, which was immersed for about 6 days as a 200-fold dilution, was recognized as a sufficient disinfecting effect.
（２）ばか苗病に対する防除価
表中No5では種子消毒剤について約20倍希釈として約24時間浸漬処理したものの防除価は93.1％であり、非常に有効であった。
(2) Control value against scab seedling disease In No5 in the table, the seed disinfectant was diluted about 20 times and immersed for about 24 hours, and the control value was 93.1%, which was very effective.
（３）いもち病に対する胞子形成もみ率
表中No1では種子消毒剤について約５倍希釈として約30分浸漬処理したものの胞子形成もみ率は０％であり、No2では種子消毒剤について約５倍希釈として約１時間浸漬処理したものの胞子形成もみ率は０％であり、No3では種子消毒剤について約10倍希釈として約１時間浸漬処理したものの胞子形成もみ率は0.3％であり、No5では種子消毒剤について約20倍希釈として約24時間浸漬処理したものの胞子形成もみ率は０％であり、No6では種子消毒剤について約40倍希釈として約24時間浸漬処理したものの胞子形成もみ率は０％であり、非常に有効であった。これらは、いもち病に対する有効処理濃度といえるものである。
(3) Spore formation rate for rice blast disease No. 1 in the table is about 5 times diluted for seed disinfectant and soaked for about 30 minutes, the rate of spore formation is 0%, and No 2 is about 5 times diluted for seed disinfectant In the case of No.3, the spore formation rate was 0%. In No3, the seed disinfectant was diluted about 10 times and the immersion rate was 0.3%. In No5, the seed disinfection rate was 0.3%. The spore formation rate was 0% when the agent was soaked for about 24 hours with a 20-fold dilution, and the spore formation rate was 0% when the soaked agent was soaked for about 24 hours with the No. 6 seed disinfectant. Yes, it was very effective. These are effective treatment concentrations for blast.
（４）ごま葉枯病に対する胞子形成阻止率
表中No5では種子消毒剤について約20倍希釈として約24時間浸漬処理したものの胞子形成阻止率は95.4％であり、非常に有効であった。
(4) Spore formation inhibition rate against sesame leaf blight No. 5 in the table was a very effective spore formation inhibition rate of 95.4% when the seed disinfectant was diluted by about 20 times and immersed for about 24 hours.
また、病害によって有効な処理条件に違いがあるが、糸状菌であるいもち病、ごま葉枯病、ばか苗病に対しては、種子消毒剤について約20倍希釈（濃度）として約24時間浸漬処理（浸漬時間）が共通して有効であった。 In addition, there are differences in effective treatment conditions depending on the disease, but for the rice blast disease, sesame leaf blight disease, and idiot seedling disease that are filamentous fungi, the seed disinfectant is immersed for about 24 hours as a 20-fold dilution (concentration). Treatment (dipping time) was effective in common.
上記のように、表中の胞子形成もみ率、胞子形成阻止率、防除価の数値により病害菌の発育を抑制する効果が把握でき、種子消毒効果があることがわかる。 As described above, it can be understood that the effect of suppressing the growth of disease-causing fungi can be grasped by the numerical values of the spore formation rate, the spore formation inhibition rate and the control value in the table, and it can be seen that there is a seed disinfection effect.
天然由来の木酢液を有効成分とし、細菌性病害（イネ苗立枯細菌病、もみ枯細菌病、褐条病）や糸状菌性病害（ばか苗病、いもち病、ごま葉枯病）などへの罹病を抑制することができるので、従来ほど環境に悪い影響を与えることなくイネの種子などを消毒することができる種子消毒剤及び種子消毒方法を提供することができるができ、植物の種子を消毒する種々の用途に適用することができる。 Naturally-derived wood vinegar is used as an active ingredient, for bacterial diseases (rice seedling blight, rice blight, brown streak) and filamentous fungi (baka seedling, rice blast, sesame leaf blight) The seed disinfectant and the seed disinfection method that can disinfect rice seeds and the like without adversely affecting the environment as in the past can be provided. It can be applied to various uses for disinfection.
Claims (5)
細菌性病害、糸状菌性病害の少なくとも１つ以上を防除する 種子消毒剤であって、
木質原料を乾留することによって生じた液状物を減圧蒸留し、
減圧蒸留して得られた粗蒸留液を静置する事により、
軽質油層、水層、沈降タール層の三層に分離させ、前記水層を抜き出し、
抜き出された粗木酢液に少なくとも２回の常圧蒸留を行い、
木酢液の主成分である酢酸を含む留分を回収するようにし、
酢酸濃度が４〜１０％の木酢液となるようにした
ことを特徴とする種子消毒剤。 Dilute wood vinegar, soak rice seeds in diluted wood vinegar,
A seed disinfectant for controlling at least one of bacterial diseases and fungal diseases,
The liquid material produced by dry distillation of the woody material is distilled under reduced pressure,
By leaving the crude distillate obtained by distillation under reduced pressure,
The light oil layer, the water layer, and the precipitated tar layer are separated into three layers, and the water layer is extracted.
Perform at least two atmospheric distillations on the extracted crude wood vinegar ,
So that the fraction containing acetic acid, the main component of the wood vinegar, is collected,
A seed disinfectant characterized in that the concentration of acetic acid is 4-10% pyroligneous acid .
もみ枯細菌病、苗立枯細菌病、褐条病、いもち病、ばか苗病の少なくとも１つ以上であるAt least one of Bacterial wilt disease, Bacterial wilt disease, Brown streak disease, Rice blast disease, Baka seedling disease
ことを特徴とする請求項３記載の種子消毒剤。The seed disinfectant according to claim 3.
ことを特徴とする請求項４記載の種子消毒剤。The seed disinfectant according to claim 4, wherein
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| JP2005350408A (en) * | 2004-06-11 | 2005-12-22 | Kyooei:Kk | Disinfectant and sterilization method for seed rice |
| JP5236339B2 (en) * | 2008-04-08 | 2013-07-17 | 学校法人 京都産業大学 | Avian influenza virus inactivator |
| JP5787283B2 (en) * | 2008-04-10 | 2015-09-30 | クミアイ化学工業株式会社 | Rice soil disease control agent |
| CN104770104B (en) * | 2015-04-17 | 2017-03-01 | 山东省分析测试中心 | A kind of method promoting Seeds pression to sprout |
| CN104798485B (en) * | 2015-04-17 | 2017-03-01 | 山东省分析测试中心 | A kind of method promoting Rhizoma Anemarrhenae seed to sprout |
| CN104838756B (en) * | 2015-04-17 | 2017-03-01 | 山东省分析测试中心 | A kind of method promoting Seeds of Trichosanthes Kirilowii to sprout |
| CN104798487B (en) * | 2015-04-17 | 2017-03-01 | 山东省分析测试中心 | A kind of method promoting seed germination of Isatis Indigotica Fort |
| CN104770105B (en) * | 2015-04-17 | 2017-03-01 | 山东省分析测试中心 | A kind of method promoting Radix Scutellariae seed to sprout |
| CN104798486B (en) * | 2015-04-17 | 2017-03-01 | 山东省分析测试中心 | A kind of method promoting astragalus membranaceus seed to sprout |
| CN105144912B (en) * | 2015-10-15 | 2017-12-22 | 重庆市中药研究院 | A kind of method that largeflower-like honeysuckle flower seed is sprouted |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52102165A (en) * | 1976-02-20 | 1977-08-26 | Chugai Pharmaceutical Co Ltd | Germination and rooting stimulater for useful plants |
| JPH04145194A (en) * | 1990-10-04 | 1992-05-19 | Nippon Raito Fuoosu Kk | Ionically activated diluted pyrolignous acid |
| JPH1160422A (en) * | 1997-08-26 | 1999-03-02 | Rontai Kk | Seeds coated with coating substance containing pyroligneous acid |
| JP2000316318A (en) * | 1999-05-11 | 2000-11-21 | Tachibana Paper Wear Kk | Far-infrared treatment of seed rice |
| JP2002088374A (en) * | 2000-09-12 | 2002-03-27 | Daiko Tekku Kk | Method for producing pyroligneous acid |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52102165A (en) * | 1976-02-20 | 1977-08-26 | Chugai Pharmaceutical Co Ltd | Germination and rooting stimulater for useful plants |
| JPH04145194A (en) * | 1990-10-04 | 1992-05-19 | Nippon Raito Fuoosu Kk | Ionically activated diluted pyrolignous acid |
| JPH1160422A (en) * | 1997-08-26 | 1999-03-02 | Rontai Kk | Seeds coated with coating substance containing pyroligneous acid |
| JP2000316318A (en) * | 1999-05-11 | 2000-11-21 | Tachibana Paper Wear Kk | Far-infrared treatment of seed rice |
| JP2002088374A (en) * | 2000-09-12 | 2002-03-27 | Daiko Tekku Kk | Method for producing pyroligneous acid |
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