JP4644478B2 - Verticillium lecani YK-920 strain and pest and plant disease control agent using the same - Google Patents
Verticillium lecani YK-920 strain and pest and plant disease control agent using the same Download PDFInfo
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Description
本発明はバーティシリウム・レカニＹＫ−９２０（ＦＥＲＭ Ｐ−２０２８５）菌株およびその利用に関し、さらに詳しくは害虫防除効果および植物病害の発病抑制効果を有するバーティシリウム・レカニ菌株を有効菌として含有する害虫防除剤又は／及び植物病害防除剤に関するものである。 The present invention relates to a Verticillium lecani YK-920 (FERM P-20285) strain and its use, and more specifically, contains Verticillium lecani strain having an insect pest control effect and an effect of suppressing plant disease as an effective bacterium. The present invention relates to a pest control agent and / or a plant disease control agent.
現在の植物の病害虫の防除には、化学農薬を用いる化学的防除が主流を占めている。化学農薬である殺虫剤や殺菌剤では様々な作用点を持ち、高い効果を有する薬剤が多数開発され、更に多くの施用法も同時に開発されてきた。これにより化学農薬を用いる化学的防除は植物の病害虫の防除と防除作業における労力の省力化に大きく貢献し、現在、広く普及している。しかし一方でこれらの化学農薬を用いた防除法には、耐性を持った病害虫の出現による防除効果の低下等の問題も発生しており、化学農薬に依存しない防除法の開発も強く望まれるようになってきた。 Chemical control using chemical pesticides dominates the current control of plant pests. Insecticides and fungicides, which are chemical pesticides, have developed a large number of highly effective drugs with various action points, and more application methods have been developed at the same time. As a result, chemical control using chemical pesticides has greatly contributed to the control of plant pests and labor saving in control work, and is now widely used. However, on the other hand, the control methods using these chemical pesticides also have problems such as a decrease in control effect due to the emergence of resistant pests, and the development of control methods that do not depend on chemical pesticides is strongly desired. It has become.
このような要望に応えるものとして、近年、自然界から採集された微生物を利用した生物的防除方法が提案され、微生物農薬として一部実用化されてきている。 In response to such demands, in recent years, biological control methods using microorganisms collected from the natural world have been proposed and partially put into practical use as microbial pesticides.
微生物農薬を利用した害虫防除方法として昆虫病原性糸状菌である、ボーベリア（Ｂｅａｕｖｅｒｉａ）属菌、ペキロマイセス（Ｐａｅｃｉｌｏｍｙｃｅｓ）属菌、ノムライー（Ｎｏｍｕｒｅａ）属菌、及びバーティシリウム（Ｖｅｒｔｉｃｉｌｌｉｕｍ）属菌等を用いたものが知られており、現在、これら糸状菌を用いた微生物農薬がいくつか登録され、上市されている。一方、微生物農薬を利用した植物病害の防除方法としてフザリウム（Ｆｕｓａｒｉｕｍ）属菌、トリコデルマ（Ｔｒｉｃｈｏｄｅｒｍａ）属菌等の糸状菌を用いたもの、バシルス（Ｂａｃｉｌｌｕｓ）属細菌、シュードモナス（Ｐｓｕｅｄｏｍｏｎａｓ）属細菌、エルビニア（Ｅｒｗｉｎｉａ）属細菌、バークホルデリア（Ｂｕｒｋｈｏｌｄｅｒｉａ）属細菌などの細菌を用いたものが知られており、これらのいくつかも微生物農薬として登録され、上市されている。 As pest control methods using microbial pesticides, entomopathogenic filamentous fungi such as Beauveria, Paecilomyces, Nomurea, Verticillium, etc. are used. Several microbial pesticides using these filamentous fungi have been registered and marketed. On the other hand, as a method for controlling plant diseases using microbial pesticides, those using filamentous fungi such as Fusarium spp., Trichoderma spp., Bacillus spp., Pseudomonas spp., Erwinia Those using bacteria such as (Erwinia) bacteria and Burkholderia bacteria are known, and some of them are registered and marketed as microbial pesticides.
このうちバーティシリウム・レカニを用いた微生物農薬として「バータレック」、「マイコタール」が上市されている（例えば、非特許文献１）。本菌は、菌の系統によって害虫類に対する病原力や菌自体の特徴が異なることが知られており、上述したバータレックはアブラムシ類に対して高い病原性を持ち、マイコタールはコナジラミ類に対して病原性が高い系統である。また他にカイガラムシ類、アザミウマ類、ダニ類やシストセンチュウ、ネコブセンチュウに対して病原性をもつ系統、植物病原菌のさび病菌やうどんこ病菌（例えば、特許文献１）に対して拮抗作用を持つ系統についての報告がある。しかし害虫類に対して広範囲で病原性を持ち、また同時に植物病原菌に対して拮抗性を示すバーティシウム・レカニ糸状菌の系統についてはまだ見出されていない。
本発明の課題は、複数種の害虫の防除又は／及び植物病害の発病抑制の効果を有する新規な菌株を分離することにある。本発明の更なる課題は、上記菌株を有効菌として含有し、微生物農薬として有効に使用できる害虫防除剤又は／及び植物病害防除剤を提供することにある。 An object of the present invention is to isolate a novel strain having an effect of controlling a plurality of kinds of pests or / and suppressing the onset of plant diseases. It is a further object of the present invention to provide a pest control agent and / or a plant disease control agent that contains the strain as an effective bacterium and can be used effectively as a microbial pesticide.
本発明者らは、自然界から多数の菌株を分離して検定した結果、バーティシリウム属に属する１新菌株が、植物体の成長に悪影響をおよぼすことなく、数種の害虫に対して防除効果を有し、植物病害に対しても防除効果を有していることを見出し、本発明を完成した。 As a result of isolating and testing a large number of strains from nature, the present inventors have found that one new strain belonging to the genus Verticillium has a controlling effect on several types of pests without adversely affecting the growth of the plant body. The present invention was completed by finding that it has a controlling effect against plant diseases.
すなわち本発明は、害虫防除と植物病害防除の能力を有するバーティシリウム・レカニの菌株を有効菌として含有することを特徴とする病害虫防除微生物農薬である。更には、以下の菌株、並びに植物病害防除剤及び害虫防除剤を提供するものである。
（１）バーティシリウム・レカニ（Ｖｅｒｔｉｃｉｌｌｉｕｍ ｌｅｃａｎｉｉ）ＹＫ−９２０菌株。
（２）バーティシリウム・レカニ（Ｖｅｒｔｉｃｉｌｌｉｕｍ ｌｅｃａｎｉｉ）ＹＫ−９２０菌株を有効菌として含有する害虫防除剤。
（３）バーティシリウム・レカニ（Ｖｅｒｔｉｃｉｌｌｉｕｍ ｌｅｃａｎｉｉ）ＹＫ−９２０菌株を有効菌として含有する植物病害防除剤。
That is, the present invention is a pest control microbial pesticide characterized by containing, as an effective bacterium, a strain of Verticillium regani having a pest control and plant disease control ability. Furthermore, the following strains and plant disease control agents and pest control agents are provided.
(1) Verticillium lecanii YK-920 strain.
(2) A pest control agent containing Verticillium lecanii YK-920 strain as an effective bacterium.
(3) A plant disease control agent comprising Verticillium lecanii YK-920 strain as an effective bacterium.
（バーティシリウム・レカニＹＫ−９２０菌株の単離と寄託）
本発明のＹＫ−９２０菌株は静岡県小笠郡菊川町の温室においてシルバーリーフコナジラミから単離された菌株である。これらの菌株は後記する形態学的性質をもとに、青木による同定法（昆虫病原菌の検索 １９８９）を参考にして同定したところ、バーティシリウム・レカニに属する新菌株であり、本発明のＹＫ−９２０菌株は現在、独立行政法人産業技術総合研究所 特許生物寄託センターにおいて、Ｖｅｒｔｉｃｉｌｌｉｕｍ ｌｅｃａｎｉｉ ＹＫ−９２０（ＦＥＲＭ Ｐ−２０２８５）として寄託されている。
(Isolation and deposit of Verticillium lekani strain YK-920)
The YK-920 strain of the present invention is a strain isolated from silver leaf whitefly in a greenhouse in Kikukawa-cho, Ogasa-gun, Shizuoka Prefecture. These strains were identified based on the morphological properties described later with reference to the identification method by Aoki (search for insect pathogens 1989), and are new strains belonging to Verticillium regani, -920 strain is currently deposited as Verticillium lecanii YK-920 (FERM P-20285) at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology.
（バーティシリウム・レカニＹＫ−９２０の形態学的性質）
無色の分生子柄の中間部及び先端部において、突きぎり型（太い基部から先端まで均一に次第に細くなる）のフィアライドが２〜３本輪生分岐し、フィアライドの各先端には球状の胞子塊を形成する。分生子は無色、楕円形から円筒形で両端は丸く、単胞である。
(Morphological properties of Verticillium lekani YK-920)
At the middle and tip of the colorless conidia pattern, two or three pierced phialides (thinly and gradually narrowing from the thick base to the tip) branch off, and each tip of the sphere has a spherical spore mass. Form. Conidia are colorless, oval to cylindrical, rounded at both ends, and are single cells.
本発明の防除剤に用いるバーティシリウム・レカニＹＫ−９２０菌株は、ふすまなどの資材培養、固形培地上での静置培養、液体培養等の公知の手段で増殖させたものを用いればよく、生存細胞が増殖するのであれば特に培地の種類、培養条件等に制限されることはない。 The Verticillium regani YK-920 strain used for the control agent of the present invention may be a material grown by known means such as material culture such as bran, stationary culture on a solid medium, liquid culture, There are no particular restrictions on the type of culture medium, culture conditions, etc., as long as viable cells grow.
本発明のバーティシリウム・レカニＹＫ−９２０菌株は半翅目害虫、鱗翅目害虫、アザミウマ目害虫などの害虫を防除することができる。 The Verticillium regani YK-920 strain of the present invention can control pests such as Hemiptera pests, Lepidoptera pests and Thrips pests.
バーティシリウム・レカニＹＫ−９２０菌株が防除することができる害虫として、具体的には、半翅目害虫、例えばトビイロウンカ（Ｎｉｌａｐａｒｖａｔａ ｌｕｇｅｎｓ）等のウンカ類、シルバーリーフコナジラミ（Ｂｅｍｉｓｉａ ｔａｂａｃｉ）等のコナジラミ類、鱗翅目害虫、例えばチャハマキ（Ｈｏｍｏｎａ ｍａｇｎａｎｉｍａ）等のハマキガ類、コナガ（Ｐｌｕｔｅｌｌａ ｘｙｌｏｓｔｅｌｌａ）等のスガ類、オオタバコガ（Ｈｅｌｉｃｏｖｅｒｐａ ａｒｍｉｇｅｒａ）等のヤガ類、アザミウマ目害虫、例えばネギアザミウマ（Ｔｈｒｉｐｓ ｔａｂａｃｉ ）などをあげることができるが本発明はこれらの例により限定されるものではない。 Specific examples of insect pests that can be controlled by Verticillium lecani strain YK-920 include hemiptera pests such as leafhoppers such as leafhopper (Nilaparvatta lugens) and whiteflies such as silver leaf whitefly (Bemisia tabaci) Lepidopterous pests, for example, toads such as Chamonaki (Homona magnanima), Suga, such as Plutella xylostella, moths, such as Helicoverpa armigera, and Pseudomonas p. However, the present invention is not limited by these examples.
バーティシリウム・レカニＹＫ−９２０菌株は不完全菌類（Ｄｅｕｔｅｒｏｍｙｃｅｔｅｓ）に属する菌類に起因する植物の病害を防除することができる。バーティシリウム・レカニＹＫ−９２０菌株が防除することができる病気の原因菌として、具体的には、セプトリア（Ｓｅｐｔｏｒｉａ）属菌、例えばコムギふ枯病菌（Ｓｅｐｔｏｒｉａ ｎｏｄｏｒｕｍ）をあげることができるが、本発明はこれらの例により限定されるものではない。 Verticillium regani strain YK-920 can control plant diseases caused by fungi belonging to Deuteromycetes. Specific examples of causative bacteria that can be controlled by Verticillium lecani strain YK-920 include Septoria bacteria, such as Wheat fungus (Septoria nodorum). The invention is not limited by these examples.
本発明による害虫防除剤および植物病害防除剤においてバーティシリウム・レカニＹＫ−９２０菌株は、水中胞子または気中胞子水懸濁液を単独で用いるほか、不活性な液体または固体の担体で希釈し、必要に応じて界面活性剤、その他の補助剤を加えた薬剤として用いてもよい。具体的な製剤例としては、粒剤、粉剤、水和剤、懸濁製剤、乳剤等の剤型等があげられる。好ましい担体の例としては、タルク、ベントナイト、クレー、カオリン、珪藻士、ホワイトカーボン、バーミキュライト、消石灰、珪砂、硫安、尿素、多孔質な固体担体、水、イソプロピルアルコール、キシレン、シクロヘキサノン、メチルナフタレン、アルキレングリコールなどの液体担体等があげられる。界面活性剤および分散剤としては、例えばジナフチルメタンスルホン酸塩、アルコール硫酸エステル塩、アルキルアリールスルホン酸塩、リグニンスルホン酸塩、ポリオキシエチレングリコールエーテル、ポリオキシエチレンアルキルアリールエーテル、ポリオキシエチレンソルビタンモノアルキレート等があげられる。補助剤としては、カルボキシメチルセルロース、ポリエチレングリコール、プロピレングリコール、アラビアゴム、キサンタンガム等、保護剤としてはスキムミルク、スクロース、グルタミン酸、ｐＨ緩衝剤等があげられる。この場合、バーティシリウム・レカニＹＫ−９２０菌株の生菌体の量、さらには適用時期および適用量は上記生菌の場合に準じて適宜決定することができる。 In the pest control agent and plant disease control agent according to the present invention, Verticillium lecani strain YK-920 is used as an underwater spore or air spore aqueous suspension alone, or diluted with an inert liquid or solid carrier. If necessary, a surfactant and other auxiliary agents may be used as a drug. Specific examples of preparations include dosage forms such as granules, powders, wettable powders, suspension preparations, and emulsions. Examples of preferred carriers include talc, bentonite, clay, kaolin, diatomite, white carbon, vermiculite, slaked lime, quartz sand, ammonium sulfate, urea, porous solid carrier, water, isopropyl alcohol, xylene, cyclohexanone, methylnaphthalene, alkylene Examples thereof include a liquid carrier such as glycol. Examples of the surfactant and dispersant include dinaphthyl methane sulfonate, alcohol sulfate ester salt, alkyl aryl sulfonate, lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkyl aryl ether, and polyoxyethylene sorbitan. Examples include monoalkylates. Examples of auxiliary agents include carboxymethylcellulose, polyethylene glycol, propylene glycol, gum arabic, and xanthan gum, and examples of protective agents include skim milk, sucrose, glutamic acid, pH buffering agent, and the like. In this case, the amount of viable cells of Verticillium lecani YK-920 strain, and the application time and amount can be appropriately determined according to the case of the above-mentioned viable cells.
さらに、本発明の植物病害防除剤および害虫防除剤は、有効成分として必要に応じて他の殺虫剤、殺菌剤、除草剤、植物生長調節剤、肥料などのその他の成分を含むことができる。また本発明の害虫防除剤および植物病害防除剤は、バーティシリウム・レカニＹＫ−９２０菌株とともに、他の種類の菌株を含有してもよい。 Furthermore, the plant disease control agent and pest control agent of the present invention may contain other components such as other insecticides, fungicides, herbicides, plant growth regulators, fertilizers, and the like as active ingredients as necessary. In addition, the pest control agent and plant disease control agent of the present invention may contain other types of strains together with Verticillium lecani YK-920 strain.
本発明の害虫防除剤および植物病害防除剤は、そのまま直接施用するか、あるいは水などで希釈して施用することができる。害虫防除剤および植物病害防除剤の施用方法は、特に限定されず、例えば、直接植物や害虫に散布する方法、土壌に散布する方法、植物や土壌に添加する水や肥料に添加する方法などがあげられる。その他、製剤の施用量は、対象病害、対象害虫、対象作物、施用方法、発生傾向、被害の程度、環境条件、使用する剤型などによって変動するので、適宜調整されることが好ましい。 The pest control agent and plant disease control agent of the present invention can be applied directly as they are, or can be applied after dilution with water or the like. The application method of the pest control agent and the plant disease control agent is not particularly limited, for example, a method of spraying directly on plants or pests, a method of spraying on soil, a method of adding water or fertilizer added to plants or soil, etc. can give. In addition, since the dosage of the preparation varies depending on the target disease, target pest, target crop, application method, occurrence tendency, degree of damage, environmental conditions, dosage form used, etc., it is preferable to adjust appropriately.
本発明のバーティシリウム・レカニＹＫ−９２０菌株は、広い害虫防除スペクトラムを有し、複数種の害虫を防除すること、また植物病害にも防除効果を有し、植物病害を防除することができる。したがって本発明のバーティシリウム・レカニＹＫ−９２０菌株を含有する害虫防除剤および植物病害防除剤は環境に対して安全性が高く、また複数種の害虫や植物病害に対して高い防除効果を有するので、他の併用手段を用いなくても広く害虫や病害を防止することができる。 The Verticillium lecani YK-920 strain of the present invention has a broad pest control spectrum, controls a plurality of pests, has a control effect on plant diseases, and can control plant diseases. . Therefore, the pest control agent and the plant disease control agent containing the Verticillium lecani YK-920 strain of the present invention are highly safe against the environment, and have a high control effect against multiple types of pests and plant diseases. Therefore, pests and diseases can be widely prevented without using other combined means.
本発明を以下の実施例によって具体的に説明するが、本発明はこれらの実施例に限定されるものではない。 The present invention will be specifically described by the following examples, but the present invention is not limited to these examples.
（バーティシリウム・レカニＹＫ−９２０の分離・培養）
本発明のバーティシリウム・レカニＹＫ−９２０菌株は静岡県小笠郡菊川町の温室においてシルバーリーフコナジラミから単離された菌株である。詳細に述べると、静岡県小笠郡菊川町の温室内におけるキャベツの表面から、本糸状菌が寄生していたシルバーリーフコナジラミを採取し、それを滅菌水に加え、希釈系列（１，１０，１００，１０００，１００００倍希釈）を作成し、それぞれ２０ｐｐｍのナリジクス酸を加えたＰＤＡ（ポテトデキストロース寒天）平板培地に１００μｌづつ塗布し、５日後に形成されたコロニーをＰＤＡスラントへ移植し、バーティシリウム・レカニＹＫ−９２０菌株を分離した。
分離したバーティシリウム・レカニＹＫ−９２０菌株をＰＤＡ平板培地で２７℃、７日間静置培養した後、約１０ｍｌの滅菌水を加えて、滅菌した筆で気中胞子を掻きとり、菌糸をガーゼで濾して、滅菌水中に懸濁し、気中胞子懸濁液を作成した。また分離したバーティシリウム・レカニＹＫ−９２０菌株をＰＤ（ポテトデキストロース）液体培地２００ｍｌに加えて、一週間振とう培養した後、遠心集菌（７０００ｒｐｍ，４℃，５ｍｉｎ）し、滅菌水中に懸濁し、培地成分を洗浄した。この操作を２回行い、再び滅菌水中に懸濁し、水中胞子懸濁液を作成した。
(Separation and culture of Verticillium regani YK-920)
The Verticillium lecani YK-920 strain of the present invention is a strain isolated from silver leaf whiteflies in a greenhouse in Kikukawa-cho, Ogasa-gun, Shizuoka Prefecture. More specifically, silver leaf whiteflies infested with this filamentous fungus were collected from the surface of cabbage in a greenhouse in Ogasa-gun, Ogasa-gun, Shizuoka Prefecture, and added to sterilized water, and diluted series (1, 10, 100 , 1000, 10000 times dilution), and each 100 μl was applied to a PDA (potato dextrose agar) plate medium to which 20 ppm of nalidixic acid was added. -Lecany YK-920 strain was isolated.
The isolated Verticillium lecani strain YK-920 was cultured in a PDA plate medium at 27 ° C. for 7 days, then about 10 ml of sterilized water was added. And suspended in sterile water to make an air spore suspension. The isolated Verticillium lecani YK-920 strain is added to 200 ml of PD (potato dextrose) liquid medium, and cultured with shaking for one week, and then centrifuged (7000 rpm, 4 ° C., 5 min) and suspended in sterile water. It became cloudy and the medium components were washed. This operation was performed twice and suspended again in sterilized water to prepare an underwater spore suspension.
（製剤例１：水和剤）
上記のようにして得られたバーティシリウム・レカニＹＫ−９２０菌株の胞子懸濁液を８重量部、珪藻土４０重量部、クレー５０重量部、ジナフタレンジスルホン酸ナトリウム１重量部及びリグニンスルホン酸ナトリウム１重量部を混合乾燥後、粉砕して水和剤とした。
(Formulation example 1: wettable powder)
8 parts by weight of the spore suspension of Verticillium lecani YK-920 obtained as described above, 40 parts by weight of diatomaceous earth, 50 parts by weight of clay, 1 part by weight of sodium dinaphthalenedisulfonate and sodium lignin sulfonate One part by weight was mixed and dried and then pulverized to obtain a wettable powder.
（製剤例２：粒剤）
上記のようにして得られたバーティシリウム・レカニＹＫ−９２０菌株の胞子懸濁液を６重量部、ラウリルアルコール硫酸エステルのナトリウム塩１重量部、リグニンスルホン酸ナトリウム１重量部、カルボキシメチルセルロース２重量部及びクレーを９０重量部を均一に混合粉砕する。この混合物を、押出式造粒機を用いて１４〜３２メッシュの粒状に加工した後、乾燥して粒剤とした。
(Formulation Example 2: Granules)
6 parts by weight of the spore suspension of Verticillium lecani YK-920 obtained as described above, 1 part by weight of sodium salt of lauryl alcohol sulfate, 1 part by weight of sodium lignin sulfonate, 2 parts by weight of carboxymethyl cellulose 90 parts by weight of clay and clay are mixed and ground uniformly. This mixture was processed into granules of 14 to 32 mesh using an extrusion granulator and then dried to obtain granules.
次に本発明のバーティシリウム・レカニＹＫ−９２０菌株が害虫防除剤及び植物病害防除剤として有用であることを試験例で示す。 Next, it will be shown in test examples that Verticillium lecani YK-920 strain of the present invention is useful as a pest control agent and a plant disease control agent.
（試験例１：シルバーリーフコナジラミに対する殺虫効果試験）
セル苗キャベツにシルバーリーフコナジラミ成虫を放して約２４時間産卵させた。産卵されたキャベツを９日間温室内に保持し、卵から幼虫が孵化してきた状態のキャベツを上記のようにして得られたバーティシリウム・レカニＹＫ−９２０菌株の胞子懸濁液或いは水和剤（水中胞子懸濁液を使用して製剤化、以下同様）を２００倍に希釈した菌液で浸漬処理した。風乾後、プラスチック製カップに入れて飼育し、１６日後に生存虫数を数え、数１により死虫率を求めた。試験結果を表２に示した。
(Test Example 1: Insecticidal effect test on silver leaf whitefly)
Silver leaf whitefly adults were released in cell seedling cabbage and allowed to lay eggs for about 24 hours. Spawned cabbage is kept in a greenhouse for 9 days, and a spore suspension or wettable powder of Verticillium regani YK-920 strain obtained as described above is a cabbage in which larvae have hatched from eggs. (Formulation using a spore suspension in water, the same applies hereinafter) was immersed in a bacterial solution diluted 200 times. After air-drying, the animals were reared in plastic cups. After 16 days, the number of surviving insects was counted. The test results are shown in Table 2.
（数１）
死虫率（％）＝（処理前虫数−処理後生存虫数）／処理前虫数×１００
(Equation 1)
Death rate (%) = (number of pre-treatment insects−number of surviving insects after treatment) / number of pre-treatment insects × 100
（試験例２：オオタバコガに対する殺虫効果試験）
上記のようにして得られたバーティシリウム・レカニＹＫ−９２０菌株の胞子懸濁液或いは水和剤を２００倍に希釈した菌液に、直径約１２ｃｍのキャベツ葉の１／２葉片を浸漬し、風乾後、容量６０ｍｌのプラスチック製カップに入れた。その中にオオタバコガ孵化幼虫を１０頭放ち、湿らせた濾紙を入れて蓋をした。その後、２５℃の恒温室（１６Ｌ：８Ｄ）に置き、処理後６日後に生死を判定して、数１により死虫率を求めた。試験結果を表３に示した。
(Test Example 2: Insecticidal effect test against giant tobacco)
In a spore suspension of Verticillium lecani YK-920 obtained as described above or a bacterial solution obtained by diluting wettable powder 200 times, ½ leaf pieces of cabbage leaves having a diameter of about 12 cm were immersed. After air drying, it was put into a plastic cup having a capacity of 60 ml. Ten giant larvae hatched in it were released, dampened with filter paper and covered. Thereafter, the specimen was placed in a constant temperature room (16 L: 8D) at 25 ° C., 6 days after the treatment, life / death was determined, and the mortality rate was obtained from Equation 1. The test results are shown in Table 3.
（試験例３：コナガに対する殺虫効果試験）
上記のようにして得られたバーティシリウム・レカニＹＫ−９２０菌株の胞子懸濁液或いは水和剤を２００倍に希釈した菌液に、直径約１２ｃｍのキャベツ葉の１／２葉片を浸漬し、風乾後、容量６０ｍｌのプラスチック製カップに入れた。その中にコナガ２齢幼虫を１０頭放ち、濾紙を入れて蓋をした。その後、２５℃の恒温室（１６Ｌ：８Ｄ）に置き、処理後６日後に生死を判定して、数１により死虫率を求めた。試験結果を表３に示した。
(Test Example 3: Insecticidal effect test on Japanese horsetail)
In a spore suspension of Verticillium lecani YK-920 obtained as described above or a bacterial solution obtained by diluting wettable powder 200 times, ½ leaf pieces of cabbage leaves having a diameter of about 12 cm were immersed. After air drying, it was put into a plastic cup having a capacity of 60 ml. Ten second instar larvae were released in it, and filter paper was put in and covered. Thereafter, the specimen was placed in a constant temperature room (16 L: 8D) at 25 ° C., 6 days after the treatment, life / death was determined, and the mortality rate was obtained from Equation 1. The test results are shown in Table 3.
（試験例４：ネギアザミウマに対する殺虫効果試験）
プラスチック製カップの半分まで寒天を入れその上にキュウリのリーフディスクをのせ、ネギアザミウマの１齢幼虫をリーフディスクに接種した。上記のようにして得られたバーティシリウム・レカニＹＫ−９２０菌株の胞子懸濁液或いは水和剤を２００倍に希釈した菌液を自動散布装置で散布（２ｍｇ／ｃｍ２）し、逃亡防止のためにカップに蓋をした。６日後に生存虫数を数え、数１により死虫率を算出した。試験結果を表３に示した。
(Test Example 4: Insecticidal effect test on Negia thrips)
Agar was put in half of the plastic cup, and a cucumber leaf disc was placed on top of it, and the first instar larvae of Negia thrips were inoculated on the leaf disc. Spatter prevention (2 mg / cm 2 ) of spore suspension of Verticillium lecani YK-920 obtained as described above or bacterial solution diluted with 200 times wettable powder (2 mg / cm 2 ) to prevent escape The cup was capped for. Six days later, the number of surviving insects was counted, and the death rate was calculated from Equation 1. The test results are shown in Table 3.
（試験例５：コムギふ枯病防除効果試験）
直径６．０ｃｍのプラスチック製の鉢に、コムギ種子（品種：農林６１号）を１０粒づつ播種し、温室内で育成した。第２葉が展開したコムギに、上記のようにして得られたバーティシリウム・レカニＹＫ−９２０菌株の胞子懸濁液或いは水和剤を２００倍に希釈した菌液を１ポット当たり１０ｍｌ散布した。風乾後、コムギふ枯病菌（Ｓｅｐｔｏｒｉａ ｎｏｄｏｒｕｍ）の柄胞子を接種し、温室内で管理した。接種１０日後にポット全体の第１葉の発病面積を調査し、表４の基準により評価した。試験結果を表５に示した。
(Test Example 5: wheat blight control effect test)
10 seeds of wheat (variety: Norin 61) were sown in a plastic pot having a diameter of 6.0 cm and grown in a greenhouse. 10 ml per pot of a spore suspension of Verticillium regani YK-920 strain obtained as described above or a wettable powder diluted 200-fold was applied to the wheat with the second leaf developed. . After air drying, they were inoculated with spore spores of Septoria nodorum and managed in a greenhouse. Ten days after the inoculation, the diseased area of the first leaf of the entire pot was investigated and evaluated according to the criteria shown in Table 4. The test results are shown in Table 5.
上記の結果から明らかなように、本発明のバーティシリウム・レカニＹＫ−９２０菌株は幅広い各種害虫に対して高い殺虫活性を有し、また同時に植物体へ散布することにより植物病害に対しても高い防除効果を有している。
As is apparent from the above results, the Verticillium lecani YK-920 strain of the present invention has a high insecticidal activity against a wide variety of pests, and at the same time, it can be applied to plant diseases by spraying it on the plant body. High control effect.
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