JP4603261B2 - ヒト化カルシトニン遺伝子関連ペプチド受容体をコードする単離dna分子、関連する非ヒトトランスジェニック動物及びアッセイ方法 - Google Patents
ヒト化カルシトニン遺伝子関連ペプチド受容体をコードする単離dna分子、関連する非ヒトトランスジェニック動物及びアッセイ方法 Download PDFInfo
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Description
A=Ala=アラニン:コドンGCA,GCC,GCG,GCU
C=Cys=システイン:コドンUGC,UGU
D=Asp=アスパラギン酸:コドンGAC,GAU
E=Glu=グルタミン酸:GAA,GAG
F=Phe=フェニルアラニン:コドンUUC,UUU
G=Gly=グリシン:コドンGGA,GGC,GGG,GGU
H=His=ヒスチジン:コドンCAC,CAU
I=Ile=イソロイシン:コドンAUA,AUC,AUU
K=Lys=リジン:コドンAAA,AAG
L=Leu=ロイシン:コドンUUA,UUG,CUA,CUC,CUG,CUU
M=Met=メチオニン:コドンAUG
N=Asn=アスパラギン:コドンAAC,AAU
P=Pro=プロリン:コドンCCA,CCC,CCG,CCU
Q=Gln=グルタミン:コドンCAA,CAG
R=Arg=アルギニン:コドンAGA,AGG,CGA,CGC,CGG,CGU
S=Ser=セリン:コドンAGC,AGU,UCA,UCC,UCG,UCU
T=Thr=スレオニン:コドンACA,ACC,ACG,ACU
V=Val=バリン:コドンGUA,GUC,GUG,GUU
W=Trp=トリプトファン:コドンUGG
Y=Tyr=チロシン:コドンUAC,UAU。
マーモセット及びカニクイザルRAMP1cDNAクローニング−ポリメラーゼ連鎖反応(PCR)を使用して前頭脳cDNAから部分マーモセットRAMP1cDNAとカニクイザルcDNAを単離した。PCRプライマーはヒトRAMP1(5’−CTGCCAGGAGGCTAACTACG−3’[配列番号25]及び5’−CACGATGAAGGGGTAGAGGA−3’[配列番号26])に基づいて設計した。増幅反応は45秒94℃、45秒58℃、1分72℃を40サイクルとし、PLATINUM Taq PCRCNAポリメラーゼ(Invitrogen)に製造業者が推奨しているプロトコールに従った。潜在エラーを防ぐために多重サブクローンを配列決定した。
Claims (13)
- 配列番号1及び3から構成される群から選択されるヌクレオチド配列からなる、ヒト化RAMP1蛋白質をコードする精製核酸分子。
- 組換え宿主細胞でヒト化RAMP1蛋白質を発現させるための発現ベクターであって、前記発現ベクターが請求項1に記載の精製核酸分子を含む前記発現ベクター。
- 組換えヒト化RAMP1蛋白質を発現する宿主細胞であって、前記宿主細胞が請求項2に記載の発現ベクターを含む前記宿主細胞。
- 組換え宿主細胞におけるヒト化RAMP1蛋白質の発現方法であって、
(a)請求項2に記載の発現ベクターを適切な宿主細胞にトランスフェクトする段階、および
(b)前記ヒト化RAMP1蛋白質を前記発現ベクターから発現させる条件下で段階(a)の宿主細胞を培養する段階
を含む前記方法。 - 他の蛋白質を実質的に含まないヒト化RAMP1蛋白質であって、配列番号1及び3から構成される群から選択されるヌクレオチド配列によってコードされる前記ヒト化RAMP1蛋白質。
- 組換え宿主細胞内に含まれるDNA発現ベクターの産物である請求項5に記載のヒト化RAMP1蛋白質。
- 請求項6に記載のヒト化RAMP1蛋白質、及び機能的CRLR蛋白質を含む実質的に純粋な膜調製物。
- 前記蛋白質が配列番号2及び4から構成される群から選択されるアミノ酸配列からなる請求項5に記載のヒト化RAMP1蛋白質。
- 組換え宿主細胞内に含まれるDNA発現ベクターの産物である請求項8に記載のヒト化RAMP1蛋白質。
- 請求項9に記載のヒト化RAMP1蛋白質、及び機能的CRLR蛋白質を含む実質的に純粋な膜調製物。
- CRLR蛋白質が配列番号10、12及び14から構成される群から選択されるアミノ酸配列からなる請求項10に記載の膜調製物。
- CGRP受容体蛋白質のモジュレーターの同定方法であって、
(a)配列番号1及び3から構成される群から選択されるヌクレオチド配列によってコードされるヒト化RAMP1蛋白質とCRLR蛋白質を含むCGRP受容体に試験化合物を接触させる段階と、
(b)前記試験化合物がCGRP受容体蛋白質に及ぼす効果を測定する段階を含む前記方法。 - 段階(a)のヒト化RAMP1蛋白質が組換え宿主細胞内に含まれるDNA発現ベクターの産物である請求項12に記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US32529501P | 2001-09-27 | 2001-09-27 | |
PCT/US2002/030501 WO2003027252A2 (en) | 2001-09-27 | 2002-09-26 | Isolated dna molecules encoding humanized calcitonin gene-related peptide receptor, related non-human transgenic animals and assay methods |
Publications (3)
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JP2005503809A JP2005503809A (ja) | 2005-02-10 |
JP2005503809A5 JP2005503809A5 (ja) | 2005-12-22 |
JP4603261B2 true JP4603261B2 (ja) | 2010-12-22 |
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JP2003530824A Expired - Fee Related JP4603261B2 (ja) | 2001-09-27 | 2002-09-26 | ヒト化カルシトニン遺伝子関連ペプチド受容体をコードする単離dna分子、関連する非ヒトトランスジェニック動物及びアッセイ方法 |
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US (1) | US7193070B2 (ja) |
EP (1) | EP1438325B1 (ja) |
JP (1) | JP4603261B2 (ja) |
AT (1) | ATE453661T1 (ja) |
CA (1) | CA2461917C (ja) |
DE (1) | DE60234944D1 (ja) |
WO (1) | WO2003027252A2 (ja) |
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CN1642983A (zh) | 2002-01-25 | 2005-07-20 | G2治疗有限公司 | 抗C5aR抗体及其应用 |
US7220862B2 (en) | 2002-06-05 | 2007-05-22 | Bristol-Myers Squibb Company | Calcitonin gene related peptide receptor antagonists |
US7842808B2 (en) | 2002-06-05 | 2010-11-30 | Bristol-Myers Squibb Company | Anti-migraine spirocycles |
CA2487976C (en) | 2002-06-05 | 2011-07-26 | Bristol-Myers Squibb Company | Calcitonin gene related peptide receptor antagonists |
WO2004097421A2 (en) * | 2003-04-29 | 2004-11-11 | Bayer Healthcare Ag | Diagnostics and therapeutics for diseases associated with calcitonin receptor-like receptor (calcrl) |
JP2007524568A (ja) | 2003-12-05 | 2007-08-30 | ブリストル−マイヤーズ スクイブ カンパニー | カルシトニン遺伝子関連ペプチドレセプターアンタゴニスト |
TW200524601A (en) | 2003-12-05 | 2005-08-01 | Bristol Myers Squibb Co | Heterocyclic anti-migraine agents |
EP2322637B1 (en) * | 2003-12-24 | 2016-05-18 | Novo Nordisk A/S | Transgenic mouse comprising a polynucleotide encoding human or humanized C5AR |
TW200533398A (en) | 2004-03-29 | 2005-10-16 | Bristol Myers Squibb Co | Novel therapeutic agents for the treatment of migraine |
US7384930B2 (en) | 2004-11-03 | 2008-06-10 | Bristol-Myers Squibb Company | Constrained compounds as CGRP-receptor antagonists |
US7384931B2 (en) | 2004-11-03 | 2008-06-10 | Bristol-Myers Squibb Company | Constrained compounds as CGRP-receptor antagonists |
US7449586B2 (en) | 2004-12-03 | 2008-11-11 | Bristol-Myers Squibb Company | Processes for the preparation of CGRP-receptor antagonists and intermediates thereof |
FR2882628B1 (fr) * | 2005-03-04 | 2011-03-18 | Centre Nat Rech Scient | Souris transgeniques et leurs applications comme modele experimental |
US7834007B2 (en) | 2005-08-25 | 2010-11-16 | Bristol-Myers Squibb Company | CGRP antagonists |
ME00419B (me) | 2005-11-14 | 2011-10-10 | Rinat Neuroscience Corp | Antigonistička antitijela usmjerena protiv kalcitonita, peptida povezanog sa genom i postupkom njihovog korišćenja |
JP2009201355A (ja) * | 2006-06-02 | 2009-09-10 | Osaka Univ | 高血圧モデル非ヒト動物 |
US8337852B2 (en) | 2006-08-22 | 2012-12-25 | G2 Inflammation Pty Ltd | Anti-C5aR antibodies with improved properties |
JP2010536326A (ja) * | 2007-04-26 | 2010-12-02 | アキリオン ファーマシューティカルズ,インコーポレーテッド | Hcvのキメラns4aタンパク質を発現する細胞およびアッセイ法におけるその使用 |
ES2562627T3 (es) | 2008-02-20 | 2016-03-07 | Novo Nordisk A/S | Anticuerpos anti-C5aR humanizados |
NZ587292A (en) | 2008-03-04 | 2012-09-28 | Pfizer Ltd | Use of anti-CGRP (calcitonin gene-related peptide) antagonists to treat chronic pain |
JO3382B1 (ar) | 2008-12-23 | 2019-03-13 | Amgen Inc | أجسام مضادة ترتبط مع مستقبل cgrp بشري |
BR112012004464B8 (pt) | 2009-08-28 | 2021-05-25 | Labrys Biologics Inc | uso de um anticorpo antagonista anti-cgrp para preparar uma composição farmacêutica para tratar e/ou prevenir dor visceral e/ou um ou mais sintomas de dor visceral, em que a dor visceral está associada á cistite intersticial (ic), e dita composição farmacêutica |
CA2838497C (en) | 2011-06-06 | 2023-02-28 | Novo Nordisk A/S | Therapeutic antibodies |
JP6267986B2 (ja) * | 2014-02-13 | 2018-01-24 | 株式会社特殊免疫研究所 | ヒトの特定分子と結合する分子標的物質のinvivo評価法 |
US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
CA2943551A1 (en) | 2014-03-21 | 2015-09-24 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
UY36302A (es) | 2014-09-15 | 2016-04-29 | Amgen Inc | Proteína de unión a antígenos, bi-específicos del receptor anti-cgrp/receptor pac1 y usos de las mismas |
JOP20200116A1 (ar) | 2015-04-24 | 2017-06-16 | Amgen Inc | طرق لعلاج أو الوقاية من الصداع النصفي |
WO2018055574A1 (en) | 2016-09-23 | 2018-03-29 | Teva Pharmaceuticals International Gmbh | Treating refractory migraine |
SG11202009629YA (en) | 2018-04-02 | 2020-10-29 | Amgen Inc | Erenumab compositions and uses thereof |
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US4908678A (en) * | 1986-10-08 | 1990-03-13 | Semiconductor Energy Laboratory Co., Ltd. | FET with a super lattice channel |
US5081513A (en) * | 1991-02-28 | 1992-01-14 | Xerox Corporation | Electronic device with recovery layer proximate to active layer |
US5606177A (en) * | 1993-10-29 | 1997-02-25 | Texas Instruments Incorporated | Silicon oxide resonant tunneling diode structure |
US6255150B1 (en) * | 1997-10-23 | 2001-07-03 | Texas Instruments Incorporated | Use of crystalline SiOx barriers for Si-based resonant tunneling diodes |
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- 2002-09-26 WO PCT/US2002/030501 patent/WO2003027252A2/en active Application Filing
- 2002-09-26 DE DE60234944T patent/DE60234944D1/de not_active Expired - Lifetime
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US7193070B2 (en) | 2007-03-20 |
CA2461917C (en) | 2012-01-17 |
EP1438325B1 (en) | 2009-12-30 |
JP2005503809A (ja) | 2005-02-10 |
EP1438325A2 (en) | 2004-07-21 |
EP1438325A4 (en) | 2005-02-23 |
DE60234944D1 (de) | 2010-02-11 |
CA2461917A1 (en) | 2003-04-03 |
US20040197859A1 (en) | 2004-10-07 |
WO2003027252A3 (en) | 2003-06-19 |
ATE453661T1 (de) | 2010-01-15 |
WO2003027252A2 (en) | 2003-04-03 |
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