JP4602945B2 - 核酸の同定のための後pcr特性の複合した非対称pcr - Google Patents
核酸の同定のための後pcr特性の複合した非対称pcr Download PDFInfo
- Publication number
- JP4602945B2 JP4602945B2 JP2006179847A JP2006179847A JP4602945B2 JP 4602945 B2 JP4602945 B2 JP 4602945B2 JP 2006179847 A JP2006179847 A JP 2006179847A JP 2006179847 A JP2006179847 A JP 2006179847A JP 4602945 B2 JP4602945 B2 JP 4602945B2
- Authority
- JP
- Japan
- Prior art keywords
- probe
- nucleic acid
- probes
- primer
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
- C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Pathology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
EFFGGAALLGFFAGGAFGAFFGGGTCCTJ(配列ID番号3)
ただしE=BHQ2;J=cx-FAM;F=プロピニルdU;L=プロピニルdCである。
Claims (10)
- サンプル中の1つ以上の核酸標的を同定する方法であって、
a)標識した1つ以上の5’−ヌクレアーゼ・プローブと、標識した1つ以上のハイブリダイゼーション・プローブとを含む反応混合物の中で非対称動的PCRを実施し、ここで5’−ヌクレアーゼ・プローブとハイブリダイゼーション・プローブは同じプローブでも異なるプローブでもよく;
b)その動的PCRから得られる1つ以上の増加曲線をモニターし;
c)その動的PCRの後に反応混合物の温度を変化させることにより、標識した1つ以上の上記ハイブリダイゼーション・プローブと1つ以上の核酸標的の間の会合を変化させ;
d)標識した1つ以上の上記ハイブリダイゼーション・プローブから発生する1つ以上の蛍光信号をモニターすることにより、融解曲線またはアニーリング曲線を作り;
e)ステップd)の融解曲線またはアニーリング曲線を、1つ以上の既知の核酸標的からなる完全に相補的なプローブの融解曲線またはアニーリング曲線と相関させることで、サンプル中の1つ以上の核酸標的を同定する操作を含み、
ここで前記非対称動的PCRが、1つの第1のプライマーと少なくとも1つの第2のプライマーを含んでいて、第1のプライマーの量が第2のプライマーの量よりも多く、上記ハイブリダイゼーション・プローブが、第2のプライマーよりも多い量存在している、方法。 - 1つ以上の核酸標的を同定する上記操作が、その核酸を含む1つ以上の生物または生物株を同定する操作を含む、請求項1に記載の方法。
- 上記非対称動的PCRが、1つの第1のプライマーと少なくとも1つの第2のプライマーを含んでいて、このPCRが、第1のプライマーを第2のプライマーの2倍以上多く含んでいる、請求項1に記載の方法。
- 1つ以上の上記ハイブリダイゼーション・プローブが、動的PCRの間を通じて存在している、請求項1に記載の方法。
- 1つ以上の蛍光信号をモニターする上記操作が、ある温度範囲にわたるモニター操作を含む、請求項1に記載の方法。
- 1つ以上の上記核酸標的が、C型肝炎ウイルス(HCV)の核酸を含む、請求項1に記載の方法。
- 少なくとも1つの上記ハイブリダイゼーション・プローブが、1つのHCV株遺伝子型と相補的である、請求項1に記載の方法。
- 上記反応混合物が、第1のハイブリダイゼーション・プローブと、少なくとも1つの第2のハイブリダイゼーション・プローブとを含んでおり、前記第1のハイブリダイゼーション・プローブが、第1のHCV株遺伝子型と相補的であり、少なくとも1つの前記第2のハイブリダイゼーション・プローブが、第2のHCV株遺伝子型と相補的である、請求項1に記載の方法。
- 配列ID番号3のポリヌクレオチド配列を有し、長さが27〜100ヌクレオチドである、標識化されたプローブ。
- 配列ID番号3のポリヌクレオチド配列から成る核酸。
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US69599105P | 2005-06-30 | 2005-06-30 | |
US69629305P | 2005-06-30 | 2005-06-30 | |
US69630305P | 2005-06-30 | 2005-06-30 | |
US69625305P | 2005-06-30 | 2005-06-30 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007014333A JP2007014333A (ja) | 2007-01-25 |
JP4602945B2 true JP4602945B2 (ja) | 2010-12-22 |
Family
ID=37104934
Family Applications (6)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006179767A Expired - Fee Related JP4638388B2 (ja) | 2005-06-30 | 2006-06-29 | 単一プローブ分析を用いるc型肝炎ウイルスタイピングのためのプローブおよび方法 |
JP2006179867A Active JP4634340B2 (ja) | 2005-06-30 | 2006-06-29 | 発光修飾剤並びに核酸検出、増幅および分析におけるその使用 |
JP2006180022A Expired - Fee Related JP5405715B2 (ja) | 2005-06-30 | 2006-06-29 | 多次元プローブ分析法によるc型肝炎ウイルス型判定のためのプローブ及び方法 |
JP2006179847A Active JP4602945B2 (ja) | 2005-06-30 | 2006-06-29 | 核酸の同定のための後pcr特性の複合した非対称pcr |
JP2010030293A Active JP5155348B2 (ja) | 2005-06-30 | 2010-02-15 | 発光修飾剤並びに核酸検出、増幅および分析におけるその使用 |
JP2013181361A Expired - Fee Related JP6026977B2 (ja) | 2005-06-30 | 2013-09-02 | 多次元プローブ分析法によるc型肝炎ウイルス型判定のためのプローブ及び方法 |
Family Applications Before (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006179767A Expired - Fee Related JP4638388B2 (ja) | 2005-06-30 | 2006-06-29 | 単一プローブ分析を用いるc型肝炎ウイルスタイピングのためのプローブおよび方法 |
JP2006179867A Active JP4634340B2 (ja) | 2005-06-30 | 2006-06-29 | 発光修飾剤並びに核酸検出、増幅および分析におけるその使用 |
JP2006180022A Expired - Fee Related JP5405715B2 (ja) | 2005-06-30 | 2006-06-29 | 多次元プローブ分析法によるc型肝炎ウイルス型判定のためのプローブ及び方法 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010030293A Active JP5155348B2 (ja) | 2005-06-30 | 2010-02-15 | 発光修飾剤並びに核酸検出、増幅および分析におけるその使用 |
JP2013181361A Expired - Fee Related JP6026977B2 (ja) | 2005-06-30 | 2013-09-02 | 多次元プローブ分析法によるc型肝炎ウイルス型判定のためのプローブ及び方法 |
Country Status (7)
Country | Link |
---|---|
US (9) | US8067208B2 (ja) |
EP (5) | EP2128269B1 (ja) |
JP (6) | JP4638388B2 (ja) |
AT (2) | ATE450623T1 (ja) |
CA (5) | CA2549905C (ja) |
DE (1) | DE602006010764D1 (ja) |
ES (3) | ES2336934T3 (ja) |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080085515A1 (en) * | 2000-03-24 | 2008-04-10 | Eppendorf Array Technologies Sa (Eat) | Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays |
US8067208B2 (en) | 2005-06-30 | 2011-11-29 | Roche Molecular Systems, Inc. | Probes and methods for hepatitis C virus typing using multidimensional probe analysis |
US7972786B2 (en) * | 2006-07-07 | 2011-07-05 | Brandeis University | Detection and analysis of influenza virus |
JP5593582B2 (ja) * | 2007-06-12 | 2014-09-24 | 東洋紡株式会社 | 核酸の迅速な検出方法 |
JP2009036687A (ja) * | 2007-08-03 | 2009-02-19 | Panasonic Corp | 遺伝子検出方法 |
US20100227314A1 (en) * | 2007-08-09 | 2010-09-09 | Dmitry Alexandrovich Gryadunov | Method of identification of genotype and subtype of hepatitis c virus on a biological microchip |
WO2009032486A1 (en) * | 2007-08-13 | 2009-03-12 | 3M Innovative Properties Company | Methods for detecting drug-resistant microbes |
US8767952B2 (en) * | 2007-12-17 | 2014-07-01 | Broadcom Corporation | Method and system for utilizing a single connection for efficient delivery of power and multimedia information |
EP2116614A1 (en) * | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
ES2439951T3 (es) * | 2008-06-06 | 2014-01-27 | F. Hoffmann-La Roche Ag | Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna |
US8658366B2 (en) * | 2008-09-18 | 2014-02-25 | Roche Molecular Systems, Inc. | Detection of target variants using a fluorescent label and a soluble quencher |
US8606527B2 (en) * | 2009-02-27 | 2013-12-10 | Bio-Rad Laboratories, Inc. | SNP detection by melt curve clustering |
US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
WO2010120800A1 (en) * | 2009-04-13 | 2010-10-21 | Canon U.S. Life Sciences, Inc. | A rapid method of pattern recognition, machine learning, and automated genotype classification through correlation analysis of dynamic signals |
CN102449166B (zh) | 2009-05-26 | 2013-12-04 | 厦门大学 | 一种单管检测多个单核苷酸变异或单核苷酸多态性的方法 |
EP2436777B1 (en) | 2009-05-26 | 2015-08-19 | Xiamen University | Method for detecting variations in nucleic acid sequences |
US8148515B1 (en) | 2009-06-02 | 2012-04-03 | Biotium, Inc. | Detection using a dye and a dye modifier |
JP2011062088A (ja) * | 2009-09-15 | 2011-03-31 | Ihi Corp | レジオネラ菌検出方法 |
US20120237451A1 (en) * | 2009-09-18 | 2012-09-20 | Antony Kuang-Shih Chen | Novel molecular beacons |
US9677125B2 (en) * | 2009-10-21 | 2017-06-13 | General Electric Company | Detection of plurality of targets in biological samples |
JP5744038B2 (ja) * | 2009-10-21 | 2015-07-01 | コーニンクレッカ フィリップス エヌ ヴェ | 増幅反応の解析ツール |
JP5623888B2 (ja) * | 2009-12-10 | 2014-11-12 | エフ.ホフマン−ラ ロシュアーゲーF.Hoffmann−La Roche Aktiengesellschaft | 分析対象物を分離して検出する方法 |
CN103069427B (zh) * | 2010-04-09 | 2016-11-23 | 生命技术公司 | qPCR基因分型数据的可视化工具 |
WO2011160887A1 (en) * | 2010-06-21 | 2011-12-29 | Siemens Healthcare Diagnostics Inc. | Soluble quencher to reduce background in qpcr assays |
US9175332B2 (en) * | 2010-07-29 | 2015-11-03 | Roche Molecular Systems, Inc. | Generic PCR |
EP2614157A4 (en) * | 2010-09-08 | 2014-03-26 | Univ Brandeis | NUCLEIC ACID DIAGNOSTIC ASSAY COMPOSITIONS AND METHODS FOR VARIABLE SEQUENCE TARGETS |
JP5722001B2 (ja) * | 2010-11-10 | 2015-05-20 | 株式会社日立ハイテクノロジーズ | 遺伝子検査方法及び検査装置 |
EP2652154B1 (en) | 2010-12-16 | 2017-10-18 | Bio-Rad Laboratories, Inc. | Universal reference dye for quantitative amplification |
US20120164641A1 (en) * | 2010-12-22 | 2012-06-28 | Roche Molecular Systems, Inc. | Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene |
CN103620389B (zh) | 2011-04-18 | 2016-08-17 | 奥林巴斯株式会社 | 目标粒子的定量方法、光分析装置以及光分析用计算机程序 |
CN103608663B (zh) * | 2011-04-20 | 2016-01-13 | 奥林巴斯株式会社 | 生物样品中的核酸分子的检测方法 |
CN103620388B (zh) * | 2011-06-27 | 2016-05-11 | 奥林巴斯株式会社 | 目标粒子的检测方法 |
WO2013040060A2 (en) * | 2011-09-12 | 2013-03-21 | Pathogenica, Inc. | Nucleic acids for multiplex detection of hepatitis c virus |
US8838394B2 (en) | 2012-02-03 | 2014-09-16 | California Institute Of Technology | Signal encoding and decoding in multiplexed biochemical assays |
CN102766701B (zh) * | 2012-07-04 | 2016-05-04 | 福州泰普生物科学有限公司 | 丙型肝炎病毒基因分型的试剂盒及方法 |
AU2013202808B2 (en) | 2012-07-31 | 2014-11-13 | Gen-Probe Incorporated | System and method for performing multiplex thermal melt analysis |
CN104662172B (zh) | 2012-08-03 | 2018-07-06 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
ES2640570T3 (es) * | 2012-10-18 | 2017-11-03 | F. Hoffmann-La Roche Ag | Ensayo de doble sonda para la detección de VHC |
EP2722397B1 (en) | 2012-10-18 | 2017-12-13 | F. Hoffmann-La Roche AG | Dual probe assay for the detection of heterogeneous amplicon populations |
CN105143847B (zh) * | 2013-02-05 | 2019-05-28 | 三路影像公司 | 细胞染色组合物及其用途 |
EP3044337B1 (en) | 2013-09-13 | 2020-03-18 | Rutgers, the State University of New Jersey | Multiplex diagnostic assay for lyme disease and other tick-borne diseases |
GB201411567D0 (en) * | 2014-06-30 | 2014-08-13 | Epistem Ltd | Quantification methods |
JP6962927B2 (ja) * | 2016-03-11 | 2021-11-05 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | ジカウイルス検出用組成物および方法 |
CN109642252A (zh) * | 2016-06-17 | 2019-04-16 | 加州理工学院 | 核酸反应及相关方法和组合物 |
AU2017346854B2 (en) * | 2016-10-19 | 2024-05-23 | Gen-Probe Incorporated | Compositions and methods for detecting or quantifying hepatitis C virus |
CN108546748A (zh) * | 2017-04-05 | 2018-09-18 | 杭州丹威生物科技有限公司 | 一种检测核酸的方法和试剂盒 |
CA3068482A1 (en) | 2017-10-03 | 2019-04-11 | Abbott Molecular Inc. | Assay for detecting hepatitis c virus (hcv) |
KR102063864B1 (ko) * | 2018-05-16 | 2020-01-08 | 사회복지법인 삼성생명공익재단 | 표면-증강 라만 산란 기반의 감염 질환 진단용 마커 검출 방법 |
CN112766296B (zh) * | 2019-11-06 | 2023-04-07 | 济南信通达电气科技有限公司 | 输电线路安全隐患目标检测模型训练方法及装置 |
WO2021236116A1 (en) * | 2020-05-20 | 2021-11-25 | Delahoussaye Kevin | Antiviral medicinal template |
JP2024500169A (ja) | 2020-12-22 | 2024-01-04 | エフ. ホフマン-ラ ロシュ アーゲー | 大ストークスシフト蛍光色素を使用して多重化リアルタイムpcrを行うための方法 |
KR102533568B1 (ko) * | 2020-12-23 | 2023-05-16 | 건국대학교 산학협력단 | 라이트 업 RNA 앱타머를 사용한 형광 기반 다중 miRNA 검출 시스템 |
KR102465706B1 (ko) * | 2020-12-23 | 2022-11-09 | 건국대학교 산학협력단 | Dna 주형 구리 나노입자 기반 fret 센서 및 이를 이용한 표적 핵산 분자의 검출 방법 |
CN114540526B (zh) * | 2022-03-23 | 2023-10-31 | 福建医科大学孟超肝胆医院(福州市传染病医院) | 对五种输入性疟原虫分型检测的引物、探针及方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050079490A1 (en) * | 1999-12-23 | 2005-04-14 | Roche Diagnostics Corporation | Method for quickly detecting microbial dna/rna, kit therefor and the use of said method |
Family Cites Families (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
US5216141A (en) | 1988-06-06 | 1993-06-01 | Benner Steven A | Oligonucleotide analogs containing sulfur linkages |
US5386023A (en) | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
US6251581B1 (en) * | 1991-05-22 | 2001-06-26 | Dade Behring Marburg Gmbh | Assay method utilizing induced luminescence |
DE69223562T2 (de) | 1991-08-27 | 1998-06-04 | Hoffmann La Roche | Verfahren und Reagenzien zum Nachweis von Hepatitis C |
EP0637965B1 (en) | 1991-11-26 | 2002-10-16 | Isis Pharmaceuticals, Inc. | Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines |
TW393513B (en) | 1991-11-26 | 2000-06-11 | Isis Pharmaceuticals Inc | Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines |
US5484908A (en) | 1991-11-26 | 1996-01-16 | Gilead Sciences, Inc. | Oligonucleotides containing 5-propynyl pyrimidines |
US5644048A (en) | 1992-01-10 | 1997-07-01 | Isis Pharmaceuticals, Inc. | Process for preparing phosphorothioate oligonucleotides |
DE69334357D1 (de) * | 1992-11-27 | 2011-06-09 | Innogenetics Nv | Nachweis und Typisierung das Hepatitis C Virus (HCV) mittels 5'UTR und NS5 Nukleinsäuresequenzen |
EP0984068B1 (en) | 1993-04-27 | 2012-03-28 | Innogenetics N.V. | Sequences of hepatitis c virus genotypes and their use as therapeutic and diagnostic agents |
US5882852A (en) * | 1993-06-29 | 1999-03-16 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Hepatitic C virus (HCV) core gene nucleotide sequences and related methods of detecting major and minor genotypes of HCV isolates |
US5637684A (en) | 1994-02-23 | 1997-06-10 | Isis Pharmaceuticals, Inc. | Phosphoramidate and phosphorothioamidate oligomeric compounds |
US5491063A (en) | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
BR9509421A (pt) | 1994-10-21 | 1997-09-30 | Innogenetics Nv | Novas sequências de genotipos de vírus de hepatite C e uso como agentes profiláticos terapêuticos e diagnósticos |
US5837442A (en) * | 1995-11-29 | 1998-11-17 | Roche Molecular Systems, Inc. | Oligonucleotide primers for amplifying HCV nucleic acid |
US5795729A (en) * | 1996-02-05 | 1998-08-18 | Biometric Imaging, Inc. | Reductive, energy-transfer fluorogenic probes |
DE69827060T2 (de) | 1997-03-20 | 2005-03-24 | F. Hoffmann-La Roche Ag | Modifizierte Primer |
EP1009852A2 (en) | 1997-09-04 | 2000-06-21 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof |
JPH11148521A (ja) | 1997-11-13 | 1999-06-02 | Nichias Corp | 鳴き防止シム |
GB9725197D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
US6140054A (en) * | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
IL145422A0 (en) | 1999-03-18 | 2002-06-30 | Exiqon As | One step sample preparation and detection of nucleic acids in complex in biological samples |
IL145496A0 (en) | 1999-03-24 | 2002-06-30 | Exiqon As | Improved synthesis of [2.2.1] bicyclo nucleosides |
US6734291B2 (en) | 1999-03-24 | 2004-05-11 | Exiqon A/S | Synthesis of [2.2.1]bicyclo nucleosides |
US6472156B1 (en) * | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
EP1081495B1 (en) | 1999-09-01 | 2004-03-10 | Invitrogen Corporation | Photon reducing agents for fluorescence assays |
WO2001046469A1 (fr) | 1999-12-22 | 2001-06-28 | Otsuka Pharmaceutical Co., Ltd. | Procede d'etude du genotype du virus de l'hepatite c |
CN1296345C (zh) | 1999-12-27 | 2007-01-24 | 旭化成株式会社 | 生产甘氨酸的方法 |
US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
JP2002345467A (ja) | 2000-10-23 | 2002-12-03 | Srl Inc | C型肝炎ウイルスの型分類用プローブ及びそれを用いたc型肝炎ウイルスの型分類方法並びにそれを用いたインターフェロン投与の治療効果を予測する方法。 |
CN1322143C (zh) | 2000-12-04 | 2007-06-20 | 普里马根公司 | 检测线粒体核酸与核基因核酸比率确定药物活性和/或副作用的方法 |
CN1366067A (zh) * | 2001-01-15 | 2002-08-28 | 上海博华基因芯片技术有限公司 | 一种乙型、丙型肝炎双检基因芯片试剂盒及其制备和使用方法 |
EP1236804A1 (en) * | 2001-03-02 | 2002-09-04 | Boehringer Mannheim Gmbh | A method for determination of a nucleic acid using a control |
JP2002272475A (ja) | 2001-03-22 | 2002-09-24 | Eiken Chem Co Ltd | 蛍光偏光法による核酸増幅産物の検出方法 |
EP1379690A4 (en) | 2001-04-12 | 2004-08-18 | Biocore Co Ltd | OLIGONUCLEOTIDE CHIP COMPOSITION FOR THE ANALYSIS OF THE HEPATITIS C VIRUS GENOTYPE (HCV), AND DETECTION METHOD THEREOF |
JP2003084002A (ja) * | 2001-06-27 | 2003-03-19 | Fuji Photo Film Co Ltd | 蛍光検出におけるバックグラウンドの低減方法 |
US6800765B2 (en) * | 2001-08-02 | 2004-10-05 | Molecular Devices Corporation | Fluorescent pH indicators for intracellular assays |
US7196183B2 (en) * | 2001-08-31 | 2007-03-27 | Innogenetics N.V. | Hepatitis C virus genotype, and its use as prophylactic, therapeutic and diagnostic agent |
WO2003040367A1 (fr) | 2001-11-08 | 2003-05-15 | Sanko Junyaku Co., Ltd. | Procede de formation d'un autoassemblage au moyen d'oligonucleotides synthetises par amplification genique et procede de detection d'un autoassemblage et d'un gene |
WO2003057910A2 (en) | 2002-01-08 | 2003-07-17 | Roche Diagnostics Gmbh | Use of silica material in an amplification reaction |
US20030136921A1 (en) * | 2002-01-23 | 2003-07-24 | Reel Richard T | Methods for fluorescence detection that minimizes undesirable background fluorescence |
EP1506000B9 (en) * | 2002-05-20 | 2011-08-31 | Bristol-Myers Squibb Company | Heterocyclicsulfonamide hepatitis c virus inhibitors |
WO2004000995A2 (en) * | 2002-05-22 | 2003-12-31 | Marshall University | Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms |
AU2003261434A1 (en) | 2002-08-12 | 2004-02-25 | Bristol-Myers Squibb Company | Iminothiazolidinones as inhibitors of hcv replication |
US7195885B2 (en) * | 2002-08-12 | 2007-03-27 | Bristol-Myers Squibb Company | Hepatitis C virus assays |
GB0223563D0 (en) | 2002-10-10 | 2002-11-20 | Secr Defence | Detection system |
CN1711352A (zh) | 2002-11-13 | 2005-12-21 | 华盛顿大学 | 丙型肝炎病毒复制的高允许性细胞系 |
EP1565480B1 (en) | 2002-11-22 | 2018-08-22 | Roche Diagnostics GmbH | Detectable labeled nucleoside analogs and methods of use thereof |
EP1431398A1 (en) * | 2002-12-20 | 2004-06-23 | Evotec OAI AG | A method for detecting in a mixture an amount of analytes |
EP1594984A2 (en) * | 2003-02-18 | 2005-11-16 | Applera Corporation | Compositions and methods for multiplex analysis of polynucleotides |
WO2005028650A2 (en) | 2003-09-16 | 2005-03-31 | Sirna Therapeutics, Inc. | RNA INTERFERENCE MEDIATED INHIBITION OF HEPATITIS C VIRUS (HCV) EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
US8067208B2 (en) | 2005-06-30 | 2011-11-29 | Roche Molecular Systems, Inc. | Probes and methods for hepatitis C virus typing using multidimensional probe analysis |
-
2006
- 2006-06-23 US US11/474,092 patent/US8067208B2/en not_active Expired - Fee Related
- 2006-06-23 US US11/474,071 patent/US20070072211A1/en not_active Abandoned
- 2006-06-23 US US11/474,062 patent/US7919242B2/en active Active
- 2006-06-23 US US11/474,125 patent/US7465561B2/en not_active Expired - Fee Related
- 2006-06-27 CA CA2549905A patent/CA2549905C/en not_active Expired - Fee Related
- 2006-06-27 CA CA2549754A patent/CA2549754C/en active Active
- 2006-06-27 CA CA2549671A patent/CA2549671C/en active Active
- 2006-06-27 CA CA2549758A patent/CA2549758C/en not_active Expired - Fee Related
- 2006-06-27 CA CA2895667A patent/CA2895667C/en active Active
- 2006-06-28 EP EP09168656A patent/EP2128269B1/en active Active
- 2006-06-28 AT AT06013330T patent/ATE450623T1/de active
- 2006-06-28 AT AT09168656T patent/ATE507311T1/de active
- 2006-06-28 ES ES06013330T patent/ES2336934T3/es active Active
- 2006-06-28 EP EP06013330A patent/EP1739190B1/en active Active
- 2006-06-28 ES ES06013294.1T patent/ES2630186T3/es active Active
- 2006-06-28 EP EP06013329.5A patent/EP1746172B1/en active Active
- 2006-06-28 EP EP06013306.3A patent/EP1746171B1/en not_active Not-in-force
- 2006-06-28 EP EP06013294.1A patent/EP1746170B1/en not_active Not-in-force
- 2006-06-28 ES ES06013329.5T patent/ES2605016T3/es active Active
- 2006-06-28 DE DE602006010764T patent/DE602006010764D1/de active Active
- 2006-06-29 JP JP2006179767A patent/JP4638388B2/ja not_active Expired - Fee Related
- 2006-06-29 JP JP2006179867A patent/JP4634340B2/ja active Active
- 2006-06-29 JP JP2006180022A patent/JP5405715B2/ja not_active Expired - Fee Related
- 2006-06-29 JP JP2006179847A patent/JP4602945B2/ja active Active
-
2008
- 2008-11-11 US US12/268,821 patent/US8222003B2/en not_active Expired - Fee Related
-
2009
- 2009-09-01 US US12/551,810 patent/US8119346B2/en active Active
- 2009-09-01 US US12/551,734 patent/US8163484B2/en active Active
- 2009-09-01 US US12/552,034 patent/US8114595B2/en active Active
-
2010
- 2010-02-15 JP JP2010030293A patent/JP5155348B2/ja active Active
-
2011
- 2011-10-07 US US13/269,391 patent/US20120070820A1/en not_active Abandoned
-
2013
- 2013-09-02 JP JP2013181361A patent/JP6026977B2/ja not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050079490A1 (en) * | 1999-12-23 | 2005-04-14 | Roche Diagnostics Corporation | Method for quickly detecting microbial dna/rna, kit therefor and the use of said method |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4602945B2 (ja) | 核酸の同定のための後pcr特性の複合した非対称pcr | |
EP2228454B1 (en) | Multiplex quantitative nucleic acid amplification and melting assay | |
CN107254540B (zh) | 检测核酸差异的探针形式 | |
US20100143901A1 (en) | Nuclease-Free Real-Time Detection of Nucleic Acids | |
US11008626B2 (en) | Compositions and methods for detection of drug resistant Mycobacterium tuberculosis | |
WO2012038049A2 (en) | Amplification of distant nucleic acid targets using engineered primers | |
EP2347012B1 (en) | Detection of target variants using a fluorescent label and a soluble quencher | |
EP3397775B1 (en) | Generic method for the stabilization of specific rna | |
EP1606389B1 (en) | Methods for polynucleotide sequence detection | |
ES2365893T3 (es) | Modificadores de la emisión lumínica y usos de los mismos en la detección, amplificación y análisis de ácidos nucleicos. | |
JP2019524123A (ja) | 核酸の増幅及び検出/定量の効率を改良するためのヘルパーオリゴヌクレオチド |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20090804 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20091102 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20091106 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100204 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100518 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100816 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20100831 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20100930 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20131008 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 4602945 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |