JP4309822B2 - 殺菌剤 - Google Patents
殺菌剤 Download PDFInfo
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- JP4309822B2 JP4309822B2 JP2004220307A JP2004220307A JP4309822B2 JP 4309822 B2 JP4309822 B2 JP 4309822B2 JP 2004220307 A JP2004220307 A JP 2004220307A JP 2004220307 A JP2004220307 A JP 2004220307A JP 4309822 B2 JP4309822 B2 JP 4309822B2
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- nisin
- fumaric acid
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- bacteria
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- 239000000417 fungicide Substances 0.000 title claims description 19
- 230000000855 fungicidal effect Effects 0.000 title claims description 13
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 198
- 239000001530 fumaric acid Substances 0.000 claims description 99
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 99
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 claims description 88
- 108010053775 Nisin Proteins 0.000 claims description 88
- 239000004309 nisin Substances 0.000 claims description 88
- 235000010297 nisin Nutrition 0.000 claims description 88
- 230000000844 anti-bacterial effect Effects 0.000 claims description 63
- 241000894006 Bacteria Species 0.000 claims description 47
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 44
- 241000192125 Firmicutes Species 0.000 claims description 32
- 239000003899 bactericide agent Substances 0.000 claims description 26
- 239000004310 lactic acid Substances 0.000 claims description 22
- 235000014655 lactic acid Nutrition 0.000 claims description 22
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 14
- 239000000645 desinfectant Substances 0.000 claims description 9
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 7
- 239000000243 solution Substances 0.000 description 30
- 241000589248 Legionella Species 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- 208000007764 Legionnaires' Disease Diseases 0.000 description 14
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- 239000007788 liquid Substances 0.000 description 7
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- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 5
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 5
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- 235000021110 pickles Nutrition 0.000 description 5
- 244000063299 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
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- 235000014347 soups Nutrition 0.000 description 4
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- 241000607142 Salmonella Species 0.000 description 3
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- 244000057717 Streptococcus lactis Species 0.000 description 3
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- 238000012790 confirmation Methods 0.000 description 3
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- 230000007423 decrease Effects 0.000 description 3
- 235000021109 kimchi Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000015067 sauces Nutrition 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 108010062877 Bacteriocins Proteins 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 230000002070 germicidal effect Effects 0.000 description 2
- 238000004898 kneading Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 244000294411 Mirabilis expansa Species 0.000 description 1
- 235000015429 Mirabilis expansa Nutrition 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 102000007327 Protamines Human genes 0.000 description 1
- 108010007568 Protamines Proteins 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- -1 fumaric acid Chemical compound 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000013536 miso Nutrition 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940048914 protamine Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Images
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- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
実験例1は、本実施例の殺菌剤、即ち、フマール酸とナイシンとの混合液がグラム陰性菌に対してどのような殺菌作用を発揮するのかを示すものである。
実験例2は、フマール酸とナイシンとの混合液がグラム陽性菌に対してどのような殺菌作用を発揮するのかを示すものである。
実験例3は、本実施例の殺菌剤を構成するフマール酸とナイシンの有効濃度範囲を確認すべく行ったものである。
実験例4は、実験例3で確認した濃度範囲のフマール酸及びナイシンの混合割合を1:1とせずに、種々変化させて調製した場合においても良好な殺菌作用が発揮されるかどうかを確認すべく行ったものであり、具体的には実験例3で確認した濃度範囲のフマール酸及びナイシンを、種々の混合割合で混合し、レジオネラ菌及び黄色ブドウ球菌に接触させた(下記表5,表6参照)。
実験例5は本実施例の殺菌剤に乳酸を加えることで、フマール酸の濃度が実験例3において確認された下限濃度以下であっても殺菌作用を良好に発揮できることを確認したものである。
先ず、75%エタノール溶液にフマール酸を4%(w/v)となるようにして溶かす。
先ず、フマール酸を湯温に溶かし0.2%(w/v)溶液を作り、ここへ乳酸を0.8%(w/v)となるように滴下混合し、希釈してフマール酸の濃度が0.03%乃至0.1%(w/v)の範囲の溶液を作る。
先ず、味噌工場等、大豆加工工場から廃出された大豆煮汁にブドウ糖及び食塩を各々3%ずつ加え溶解する。
レジオネラ菌が750個/100ml、黄色ブドウ球菌が3×102個/g、及び、バチルス菌が7×105個/ml検出された家庭用循環風呂の浴槽水200リットル(40℃)にフマール酸100gを溶かした後、ナイシンを10g溶かし、3時間循環して菌の検出を行った。尚、この場合の浴槽水中のフマール酸の濃度は0.05%(w/v),ナイシン濃度は50ppmである。
へたを取りよく水洗いした十全ナス4個(245g)をポリ袋に入れ、該ポリ袋の中に上記第三例の殺菌剤を300ml(粉末調味料及びpH調整剤(pH5.0)を適量溶解した。)を加えて該ポリ袋を密封し、10℃で4日間保存した。
食塩3%で下漬した白菜を8kgを用意し、別に市販のキムチ漬けの素で調整したタレ1.0kgに、上記第一例の殺菌剤1リットルを混合し、ここに食塩60gを添加してタレ全体を良く混合した後、白菜にタレをまぶしてポリ袋に入れ密封し、10℃で4日間保存した。
先ず、キャベツ1kgを常法により切断、水洗いし、該切断したキャベツを次亜塩素酸ソーダ水及びビタミンC液に浸漬した。
先ず、常法により麺を茹で上げ、該麺を冷流水により冷やした。この麺200gを上記第一例のフマール・ナイシン混合液に約10秒程浸漬して引き上げ、直ちにポリ袋包装した。
上記第一乃至第三例のフマール酸・ナイシン混合液を、汁器類や調理器具の殺菌に用いることで、汁器類や調理器具の殺菌が良好に行われることを実験により確認している。
Claims (4)
- グラム陰性菌に対して殺菌作用を有するフマール酸と、グラム陽性菌に対して殺菌作用を有するナイシンと、乳酸と、リン酸及びクエン酸の双方若しくはいずれか一方とを有する殺菌剤であって、前記フマール酸の濃度は0.03%乃至0.1%(w/v)、前記ナイシンの濃度は1ppm乃至100ppmに設定されており、この殺菌剤は前記グラム陰性菌及びグラム陽性菌の双方を殺菌するものであることを特徴とする殺菌剤。
- 請求項1記載の殺菌剤において、前記フマール酸の濃度を0.1%(w/v)としたことを特徴とする殺菌剤。
- 請求項1,2いずれか1項に記載の殺菌剤において、前記ナイシンの濃度を100ppmとしたことを特徴とする殺菌剤。
- 請求項1〜3いずれか1項に記載の殺菌剤において、前記乳酸の濃度を0.8%(w/v)としたことを特徴とする殺菌剤。
Priority Applications (1)
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JP2004220307A JP4309822B2 (ja) | 2003-12-17 | 2004-07-28 | 殺菌剤 |
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JP2003420186 | 2003-12-17 | ||
JP2004220307A JP4309822B2 (ja) | 2003-12-17 | 2004-07-28 | 殺菌剤 |
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JP2005200397A JP2005200397A (ja) | 2005-07-28 |
JP4309822B2 true JP4309822B2 (ja) | 2009-08-05 |
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JP2004220307A Expired - Lifetime JP4309822B2 (ja) | 2003-12-17 | 2004-07-28 | 殺菌剤 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014077188A1 (ja) | 2012-11-14 | 2014-05-22 | 株式会社優しい研究所 | 抗菌用組成物 |
Families Citing this family (1)
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JP7352719B2 (ja) * | 2020-02-27 | 2023-09-28 | 日本曹達株式会社 | 殺菌剤組成物 |
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2004
- 2004-07-28 JP JP2004220307A patent/JP4309822B2/ja not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014077188A1 (ja) | 2012-11-14 | 2014-05-22 | 株式会社優しい研究所 | 抗菌用組成物 |
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