JP3962715B2 - 皮膚および軟組織の欠損修復のための自己皮膚繊維芽細胞の使用 - Google Patents
皮膚および軟組織の欠損修復のための自己皮膚繊維芽細胞の使用 Download PDFInfo
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- JP3962715B2 JP3962715B2 JP2003345565A JP2003345565A JP3962715B2 JP 3962715 B2 JP3962715 B2 JP 3962715B2 JP 2003345565 A JP2003345565 A JP 2003345565A JP 2003345565 A JP2003345565 A JP 2003345565A JP 3962715 B2 JP3962715 B2 JP 3962715B2
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Description
T., 1993, Clin. Plastic Surgery 20: 181-9に記載されている。
Surgery 33: 171。多くの開業医の経験によれば、約4〜6ヶ月後に顕著な吸収がある。
Oncol. 20: 586-90。FIBREL(登録商標)治療の報告では、これは一部の患者に有益(例えば、Millikan, L., et al., 1991, J. Dermatologic Surg. Oncol. 17: 223-29を参照)であるが、塊と持続性の欠如とからくる問題があると述べられている。注入に伴う不快感と、医師にとってこの調製は面倒であるという理由から、FIBREL(登録商標)の使用もまた、制限されてきた。
増殖するために、この時点で初期の培養容器から細胞をはがすことができる。
その範囲が限定されるものではなく、機能的に均等な方法および成分は本発明の範囲内にある。実際に、本明細書に記載したもの以外に本発明の種々の修飾は、上述の記載から当業者には明らかであろう。そのような修飾は、添付の請求項の範囲内に含まれる。引用したすべての文献は、参考文献として本明細書に組み込まれる。
Claims (5)
- a)ヒト被験者から得た皮膚生検試料からの皮膚繊維芽細胞を継代する;
b)無血清培地中で皮膚繊維芽細胞をインキュベートする;
c)皮膚繊維芽細胞を採取して洗浄する;および
d)繊維芽細胞を薬剤学的に許容される担体溶液に懸濁する、
ことを含んでなる、ヒト被験者における皮下または皮膚組織を増加させるための、繊維芽細胞以外の細胞および培地血清由来のタンパク質を実質的に含まない自己皮膚繊維芽細胞の懸濁液を作るための方法。 - 懸濁した繊維芽細胞および薬剤学的に許容される担体溶液と注入可能なゲルを形成するのに有効な量のヒトフィブリンとを混合させることをさらに含んでなる、請求項1に記載の方法。
- 注入可能なゲルが、トロンビン活性化同種血漿を含む、請求項2に記載の方法。
- 被験者の尋常性ざ蒼の後遺症の修復のために繊維芽細胞を接種した、繊維芽細胞以外の細胞および培地血清由来のタンパク質を実質的に含まない凝集塊を作るための方法であって、
a)被験者の真皮に由来する培養した皮膚繊維芽細胞を提供する;
b)血漿凝集塊を提供する;
c)繊維芽細胞を凝集塊の表面に加える;
d)凝集塊および繊維芽細胞を培養する;および
e)無血清培地で凝集塊および繊維芽細胞をインキュベートする、
ことを含んでなる方法。 - ヒト被験者の尋常性ざ蒼の後遺症の部位を中部または深部真皮のレベルまで掻爬することによる被験者の尋常性ざ蒼の後遺症の修復のために使用される、請求項4記載の方法で作った繊維芽細胞を接種した凝集塊であって、該凝集塊中の繊維芽細胞は自己由来であり、繊維芽細胞を接種した凝集塊は繊維芽細胞以外の細胞および培地血清由来のタンパク質を実質的に含まないことを特徴とする該凝集塊。
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CN117915933A (zh) | 2021-09-01 | 2024-04-19 | 上海其胜生物制剂有限公司 | 使用含有软骨细胞或干细胞的可注射可原位聚合胶原组合物进行软骨再生 |
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US3949073A (en) * | 1974-11-18 | 1976-04-06 | The Board Of Trustees Of Leland Stanford Junior University | Process for augmenting connective mammalian tissue with in situ polymerizable native collagen solution |
US4488911A (en) * | 1975-10-22 | 1984-12-18 | Luck Edward E | Non-antigenic collagen and articles of manufacture |
US4424208A (en) * | 1982-01-11 | 1984-01-03 | Collagen Corporation | Collagen implant material and method for augmenting soft tissue |
US4582640A (en) * | 1982-03-08 | 1986-04-15 | Collagen Corporation | Injectable cross-linked collagen implant material |
US4609551A (en) * | 1984-03-20 | 1986-09-02 | Arnold Caplan | Process of and material for stimulating growth of cartilage and bony tissue at anatomical sites |
US4642117A (en) * | 1985-03-22 | 1987-02-10 | Collagen Corporation | Mechanically sheared collagen implant material and method |
GB8708009D0 (en) * | 1987-04-03 | 1987-05-07 | Clayton Found Res | Injectable soft tissue augmentation materials |
US4969912A (en) * | 1988-02-18 | 1990-11-13 | Kelman Charles D | Human collagen processing and autoimplant use |
GB2220593B (en) * | 1988-06-09 | 1992-04-22 | Cyclofil | Hydro-cyclone |
DE3829183A1 (de) * | 1988-08-29 | 1990-03-08 | Wella Ag | Feeder-layer aus menschlichen bindegewebszellen, verfahren zu seiner herstellung sowie verfahren zur vermehrung menschlicher epithelzellen und deren verwendung zur restitution der haut |
DE3837669C2 (de) * | 1988-11-05 | 2001-12-13 | Nikolaus W Klehr | Verfahren zur Herstellung von Material für die Zelltherapie |
EP0498992A3 (en) * | 1991-02-15 | 1992-09-09 | Nippon Oil Co., Ltd. | Serum-free culture medium containing lysozyme |
WO1992017131A1 (en) * | 1991-03-29 | 1992-10-15 | Collagen Corporation | Device and method for treating facial lines |
JPH06169761A (ja) * | 1992-12-04 | 1994-06-21 | Noevir Co Ltd | 動物由来線維芽細胞の無血清培養用培地及び培養方法 |
BR9815713A (pt) * | 1997-02-20 | 2002-11-05 | Gerigene Medical Corp | Método para aumento e reparo de defeitos de tecido dérmico, subcutâneo e das cordas vocais |
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1995
- 1995-07-28 US US08/508,773 patent/US5591444A/en not_active Expired - Lifetime
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1996
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- 1996-06-06 US US08/660,783 patent/US5665372A/en not_active Expired - Lifetime
- 1996-07-03 AT AT96923640T patent/ATE250399T1/de active
- 1996-07-03 BR BR9610028-1A patent/BR9610028A/pt active IP Right Grant
- 1996-07-03 ES ES96923640T patent/ES2207679T3/es not_active Expired - Lifetime
- 1996-07-03 EP EP03011156A patent/EP1358857A1/en not_active Withdrawn
- 1996-07-03 PT PT96923640T patent/PT845963E/pt unknown
- 1996-07-03 DE DE69630129T patent/DE69630129T2/de not_active Expired - Lifetime
- 1996-07-03 DK DK96923640T patent/DK0845963T3/da active
- 1996-07-03 NZ NZ312548A patent/NZ312548A/xx not_active IP Right Cessation
- 1996-07-03 EP EP96923640A patent/EP0845963B1/en not_active Expired - Lifetime
- 1996-07-03 JP JP50759697A patent/JP3559566B2/ja not_active Expired - Lifetime
- 1996-07-03 KR KR10-1998-0700653A patent/KR100417896B1/ko not_active IP Right Cessation
- 1996-07-03 AU AU64516/96A patent/AU698440B2/en not_active Expired
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1997
- 1997-09-08 US US08/926,778 patent/US5858390A/en not_active Expired - Lifetime
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1998
- 1998-01-27 NO NO19980356A patent/NO317738B1/no not_active IP Right Cessation
- 1998-01-28 MX MX9800788A patent/MX9800788A/es unknown
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PT845963E (pt) | 2004-02-27 |
KR100417896B1 (ko) | 2005-04-20 |
US5858390A (en) | 1999-01-12 |
JP2004105742A (ja) | 2004-04-08 |
BR9610028A (pt) | 1999-12-21 |
JP3559566B2 (ja) | 2004-09-02 |
AU698440B2 (en) | 1998-10-29 |
US5660850A (en) | 1997-08-26 |
IL123077A0 (en) | 1998-09-24 |
EP0845963A1 (en) | 1998-06-10 |
AU6451696A (en) | 1997-02-26 |
CA2228138A1 (en) | 1997-02-13 |
US5665372A (en) | 1997-09-09 |
US5591444A (en) | 1997-01-07 |
NZ312548A (en) | 1999-11-29 |
NO980356D0 (no) | 1998-01-27 |
MX9800788A (es) | 1998-11-30 |
CN100349556C (zh) | 2007-11-21 |
DE69630129D1 (de) | 2003-10-30 |
CA2228138C (en) | 2007-09-11 |
ES2207679T3 (es) | 2004-06-01 |
WO1997004720A1 (en) | 1997-02-13 |
EP1358857A1 (en) | 2003-11-05 |
KR19990035989A (ko) | 1999-05-25 |
DK0845963T3 (da) | 2004-01-26 |
EP0845963A4 (en) | 2000-11-02 |
NO980356L (no) | 1998-03-19 |
JPH11510069A (ja) | 1999-09-07 |
EP0845963B1 (en) | 2003-09-24 |
IL123077A (en) | 2003-07-06 |
ATE250399T1 (de) | 2003-10-15 |
CN1198090A (zh) | 1998-11-04 |
DE69630129T2 (de) | 2004-04-08 |
NO317738B1 (no) | 2004-12-13 |
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