JP3805727B2 - Novel lactic acid strain, microbial additive containing it, and method for preparing rice fermented roughage using the additive - Google Patents
Novel lactic acid strain, microbial additive containing it, and method for preparing rice fermented roughage using the additive Download PDFInfo
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Description
【０００１】
【発明の属する技術分野】
本発明は、新規乳酸菌株、それを含有する微生物添加剤及び当該添加剤を用いる稲発酵粗飼料の調製方法に関する。
【０００２】
【従来の技術】
稲発酵粗飼料の生産は、遊休水田の有効活用と飼料自給率の向上等の観点から、普及に向けた取り組みが積極的に推進されている。
具体的には、稲発酵粗飼料向け品種の育成、専用の収穫・調製機械の開発、直播による省力・低コスト化、収穫ロスの軽減、堆肥施与による多収栽培、サイレージ給与技術への取り込みが進められている。
今後、稲発酵粗飼料の生産量は、急速に増えると考えられており、高品質の飼料を調製する技術の開発は緊急の課題となっている。
【０００３】
しかし、飼料用稲は茎が中空で、保有する空気量が多いため、サイレージ発酵過程においては糸状菌と酵母が良く増殖する。しかも、飼料用稲に付着する有効な乳酸菌が少ないため、乳酸発酵が円滑に進まず、酪酸やアンモニア態窒素が多い劣質な発酵飼料となりやすい。
稲発酵粗飼料の発酵パターンは、他の飼料作物の場合と異なっており、市販乳酸菌製剤を添加しても、発酵品質が向上しないことがしばしば見受けられる。
したがって、稲発酵粗飼料の調製にあたっては、制御された微生物発酵によって高品質の飼料を生産する必要がある。すなわち、有害微生物を抑制し、好ましい乳酸菌の増殖を促進するために、有用な乳酸菌の探索並びにその潜在機能を発掘することが極めて重要である。
【０００４】
【発明が解決しようとする課題】
本発明の目的は、上記した課題を解決するために、稲発酵粗飼料などのサイレージを調製する際の環境において、乳酸発酵能が高く、有害微生物を抑えることができる乳酸菌を探索し、選抜すると共に、当該乳酸菌をサイレージ材料草に添加して高品質稲発酵粗飼料などのサイレージを調製する技術を開発し、飼料自給率の向上を目指すことである。
【０００５】
【課題を解決するための手段】
本発明者らは、飼料用稲の微生物菌種構成とサイレージ発酵過程における微生物動態を解析すると共に、稲発酵粗飼料調製用優良乳酸菌のスクリーニングを行った。すなわち、各種飼料作物とサイレージから乳酸菌を分離し、これらの生理的・生化学的性状、糖分解性状並びにサイレージの調製適性試験を実施し、優良菌株を選抜した。次いで、飼料用稲を供試し、選抜した乳酸菌を添加して飼料用稲ロールベールサイレージを調製し、サイレージ発酵過程における微生物菌叢の変遷、有機酸組成の変化及び発酵損失を経時的に追求した。
得られた結果から、ホモ発酵型乳酸菌で低ｐＨ耐性と乳酸生成能が特に優れていたラクトバチルス・プランタルム（Lactobacillus plantarum)畜草１号株の選抜に成功し、本発明を完成するに至った。さらに、当該微生物をサイレージ材料草に添加してサイレージを調製する方法についても完成した。
【０００６】
請求項１に記載の本発明は、耐酸性と乳酸発酵能に優れた乳酸菌、ラクトバチルス・プランタルム畜草１号株（ＦＥＲＭ Ｐ−１８９３０）である。
請求項２に記載の本発明は、請求項１記載の乳酸菌を含有するサイレージ調製用微生物製剤である。
請求項３に記載の本発明は、請求項２記載の微生物製剤をサイレージ材料草に添加することを特徴とするサイレージの調製方法である。
請求項４に記載の本発明は、サイレージ材料草が飼料用稲である請求項３記載の方法である。
【０００７】
【発明の実施の形態】
以下に、上記の本発明について詳しく説明する。
請求項１に記載の乳酸菌は、以下に示した方法で取得した。
栃木県下の酪農家が栽培した飼料作物から乳酸菌の分離を行った。すなわち、飼料作物の新鮮試料１０ｇをストマッカー用ビニール袋（飛竜ＫＮ２０８、旭化成（株）製）に採取し、滅菌した生理食塩水９０ｍｌを加えて１０倍希釈液とした後、この液をさらに１０8 倍まで希釈した。
この希釈液を乳酸菌用培地であるLactobacilli MRS寒天培地（DIFCO Laboratories, Detroit, USA) 及び GYP白亜寒天培地（小崎ら、「乳酸菌実験マニュアル」、1992) に塗布して、嫌気培養装置（ヒラサワ（株）製）で３７℃にて２日間培養した。
培養物から分離した乳酸菌株について、菌学的諸性質や生理的・生化学的性質等を調べ、さらにサイレージの調製適性試験を実施し、前記の乳酸菌株を選抜した。
【０００８】
ラクトバチルス・プランタルム畜草１号株は、ホモ発酵型乳酸菌で低ｐＨ耐性と乳酸生成能が特に優れている菌株である。
この菌株は、基準株と最も近縁な系統関係にあるが、糖類発酵特性や抗菌作用等において基準株と異なることから、本発明者らは新菌種であると判定し、上記の如く命名した。この菌株は、独立行政法人 産業技術総合研究所 特許生物寄託センターに寄託されており、その受託番号はＦＥＲＭ Ｐ−１８９３０である。
【０００９】
請求項２に記載の本発明は、この乳酸菌を含有するサイレージ調製用微生物製剤である。
次に、請求項３に記載の発明であるサイレージの調製方法について説明する。
栃木県と埼玉県の農家が栽培した飼料用稲（品種：はまさり、クサホナミ）を供試し、ロールベールサイレージ調製用システムを用いて、前記乳酸菌を添加して稲発酵粗飼料を調製し、サイレージ発酵過程の状況や飼料の品質等について調べ、この乳酸菌はサイレージ調製に適した微生物であることを確認した。なお、サイレージ材料草としては、請求項４に記載したように、飼料用稲が好適である。
【００１０】
本発明のサイレージ調製方法によれば、材料草に由来するヘテロ発酵型乳酸菌でなく、ホモ発酵型乳酸菌による発酵が行われるため、発酵飼料の品質が改善されるばかりでなく、サイレージのガス生成量と乾物損失率が顕著に減少する。この発酵損失の抑制は、特定の乳酸菌の添加によって他の有害微生物の活動期を短縮し、乳酸発酵が旺盛に行われた結果である。
ラクトバチルス・プランタルム畜草１号株は、乳酸桿菌であり、サイレージ発酵過程の長期にわたって増殖し、旺盛な乳酸発酵によってサイレージのｐＨを急速に低下させる。
このような乳酸の多量生成は、サイレージ発酵に対する不良微生物である酪酸菌や好気性細菌等の生育を抑え、サイレージ発酵品質を効率的に向上させることができる。
【００１１】
【実施例】
以下に、実施例により本発明を詳しく説明するが、本発明はこれらによって制限されるものではない。
実施例１
栃木県下の酪農家が栽培した飼料作物の新鮮試料１０ｇをストマッカー用ビニール袋（飛竜ＫＮ２０８、旭化成（株）製）に採取し、滅菌した生理食塩水９０ｍｌを加えて１０倍希釈液とした後、この液をさらに１０8 倍まで希釈した。
この希釈液を乳酸菌用培地であるLactbacilli MRS 寒天培地（DIFCO Laboratories, Detroit, USA) 及び GYP白亜寒天培地（小崎ら、「乳酸菌実験マニュアル」、1992) に塗布して、嫌気培養装置（ヒラサワ（株）製）で３７℃にて２日間培養した。
培養物から分離した乳酸菌株について、菌形態観測、グラム染色、胞子形成、カタラーゼ反応及び乳酸の生成に関する試験を行った。
さらに、これらの分離菌株の生理的・生化学的性状、糖分解性状及びサイレージの調製適性試験を行った。これらの結果に基づいて、ホモ発酵型乳酸菌で、低ｐＨ耐性と乳酸生成能が最も優れていたラクトバチルス・プランタルム畜草１号株を選抜することができた。
この乳酸菌の生理的・生化学的性質を第１表に示す。
【００１２】
【表１】
第１表
* 畜草1号株：ラクトバチルス・プランタルム畜草１号株
【００１３】
第１表から明らかなように、ラクトバチルス・プランタルム畜草１号株はグラム陰性で、カタラーゼ陰性、グルコースからガスを産生しないホモ発酵型で、主にＤＬ型とＬ型の乳酸異性体を生成する乳酸菌である。
ラクトバチルス・プランタルム畜草１号株は耐酸性が強く、低ｐＨ条件下でよく生育し、乳酸菌用ＭＲＳ液体培地で培養した場合、多量の乳酸を生成し、培養液の最終ｐＨは３．６程度まで低下する。
【００１４】
これらのことから、本発明に係る上記乳酸菌は、サイレージ調製用微生物製剤として適していることが分かる。
次に、ラクトバチルス・プランタルム畜草１号株の同定を行った。まず、この乳酸菌の分類学的位置を図１に示す。
【００１５】
この菌株の全領域の16S rRNA遺伝子の塩基配列を決定し、他の菌種と比較したところ、畜草１号株の分子系統位置はラクトバチルス属のクラスターにあった。
ラクトバチルス・プランタルム畜草１号株はラクトバチルス・プランタルムの基準株と最も近縁な系統関係にあり、互いに９９％以上の16S rRNAシーケンス相同性を示すため、これを上記のように同定した。
また、第２表に示したように、この菌株の糖類発酵特性は基準株と異なる。すなわち、ラクトバチルス・プランタルム畜草１号株は、ラムノース、ズルシトール、α−メチル−Ｄ−グルコシド及びエスクリンの発酵特性において基準株と相違する。
これらのことから、この菌株を新菌種と判定した。この菌株は、独立行政法人 産業技術総合研究所 特許生物寄託センターに寄託されており、その受託番号はＦＥＲＭ Ｐ−１８９３０である。
【００１６】
【表２】
第２表（その１）
【００１７】
【表３】
第２表（その２）
【００１８】
実施例２
栃木県と埼玉県の農家が栽培した飼料用稲（品種：はまさり、クサホナミ）を供試し、飼料稲収穫調製用ロールベールシステムを用いて稲発酵粗飼料の調製を行った。添加用乳酸菌は Lactobacilli MRS 液体培地（DIFCO Laboratories, Detroit, USA）に接種し、嫌気条件下で３０℃で１６時間培養した後、菌培養液を１．０×１０8 ｃｆｕ／ｍｌに調整して飼料用稲１ｋｇ当たり１ｍｌをロールベーラに装着した自動添加装置で均一に添加した。
これらロールベールサイレージは屋外（温度：１９〜３５℃）に放置して、貯蔵後１７６日後にロールベールサイレージを解体して発酵品質を分析した。稲発酵粗飼料の微生物菌種構成（貯蔵後１７６日目）を第３表に、ロールベールサイレージの発酵品質を第４表に示した。
【００１９】
【表４】
第３表
*：第１表と同じ
【００２０】
【表５】
第４表
【００２１】
第３表から明らかなように、本発明に係る乳酸菌を添加して調製したサイレージは、乳酸菌無添加の場合のサイレージに比べ、サイレージ発酵過程において、乳酸菌数が顕著に増えており、好気性細菌、酪酸菌及びバチルス属菌等の菌数が急減した。有害微生物の増殖が抑えられた理由は、乳酸の多量生成によるｐＨ低下にある。すなわち、乳酸菌添加による高い初発菌数は、他の微生物との競合でも優勢となり、しかも添加したホモ発酵型乳酸菌の強力な乳酸生成能は、ｐＨを速やかに低下させるなどにより、他の不良微生物の増殖を有効に抑制した。
さらに、本発明に係る乳酸菌は、サイレージ環境において良好な増殖を示し、サイレージ発酵過程において酪酸菌、大腸菌等の有害微生物の増殖を抑えることが判明した。
【００２２】
また、第４表から、本発明に係る乳酸菌を添加して調製したサイレージは、乳酸菌無添加の場合のサイレージに比べてｐＨ値、酢酸と酪酸の生成量並びにアンモニア態窒素含量が低下し、乳酸生成量が高まり、良質のサイレージが得られ、また、サイレージの発酵ロスが顕著に減少している。
サイレージ発酵品質の改善は、材料草に由来するヘテロ発酵型乳酸菌でなく、上記ホモ発酵型乳酸菌を用いたことに起因するものと言える。
【００２３】
【発明の効果】
本発明により、有害微生物の増殖を抑制し、好ましい乳酸菌の増殖を促進するために有用な新規乳酸菌が提供される。すなわち、サイレージ発酵の環境において乳酸菌発酵が高く、低ｐＨ耐性のホモ発酵型乳酸菌並びに抗菌性物質の生産により好気性細菌や酪酸菌等の有害微生物の増殖を抑えるホモ発酵型乳酸菌が提供され、これを含有する微生物製剤はサイレージ調製のために有用である。
さらに、本発明により、当該微生物製剤を飼料用稲等のサイレージ材料草に添加することにより高品質のサイレージを調製する方法が提供される。
したがって、本発明によれば、遊休水田の有効活用と飼料自給率の向上を図ることができる。
【図面の簡単な説明】
【図１】 ラクトバチルス・プランタルム畜草１号株と近縁乳酸菌の16S rRNA遺伝子の分子系統樹（Knucで求めた進化距離をNJ法で作成) である。 [0001]
BACKGROUND OF THE INVENTION
The present invention relates to a novel lactic acid strain, a microbial additive containing the same, and a method for preparing rice fermented roughage using the additive.
[0002]
[Prior art]
For the production of rice fermented roughage, efforts to disseminate it are actively promoted from the viewpoints of effective utilization of idle paddy fields and improvement of feed self-sufficiency rate.
Specifically, breeding of varieties for fermented rice roughage, development of dedicated harvesting and preparation machines, labor saving and cost reduction by direct sowing, reduction of harvesting loss, high-yielding cultivation by applying compost, and incorporation into silage feeding technology It is being advanced.
In the future, the production volume of rice fermented roughage is considered to increase rapidly, and the development of technology for preparing high-quality feed has become an urgent issue.
[0003]
However, since feed rice has a hollow stem and a large amount of air, filamentous fungi and yeast grow well in the silage fermentation process. Moreover, since there are few effective lactic acid bacteria adhering to the rice for feed, lactic acid fermentation does not proceed smoothly, and it tends to be an inferior fermented feed rich in butyric acid and ammonia nitrogen.
The fermentation pattern of the rice fermented roughage is different from that of other forage crops, and it is often observed that the fermentation quality does not improve even when a commercially available lactic acid bacteria preparation is added.
Therefore, when preparing a rice fermented roughage, it is necessary to produce a high quality feed by controlled microbial fermentation. That is, in order to suppress harmful microorganisms and promote the growth of preferable lactic acid bacteria, it is extremely important to search for useful lactic acid bacteria and discover their potential functions.
[0004]
[Problems to be solved by the invention]
In order to solve the above-described problems, the object of the present invention is to search for and select lactic acid bacteria having high lactic acid fermentation ability and capable of suppressing harmful microorganisms in an environment for preparing silage such as rice fermentation roughage. The aim is to improve the feed self-sufficiency by developing a technology for preparing silage such as high quality rice fermented roughage by adding the lactic acid bacteria to the silage material grass.
[0005]
[Means for Solving the Problems]
The present inventors analyzed the microbial species composition of feed rice and the microbial behavior in the silage fermentation process, and screened excellent lactic acid bacteria for preparing rice fermented roughage. That is, lactic acid bacteria were isolated from various forage crops and silage, and the physiological / biochemical properties, saccharolytic properties, and silage preparation suitability tests were conducted, and excellent strains were selected. Next, feed rice was tested, selected lactic acid bacteria were added to prepare feed rice roll bale silage, and microbial flora change, organic acid composition change and fermentation loss were pursued over time in the silage fermentation process .
From the results obtained, we succeeded in selecting Lactobacillus plantarum No. 1 strain which was a homofermentative lactic acid bacterium and was particularly excellent in low pH tolerance and lactic acid production ability, and completed the present invention. . Furthermore, a method for preparing silage by adding the microorganism to silage material grass was also completed.
[0006]
The present invention according to
The present invention according to claim 2 is a microbial preparation for silage preparation containing the lactic acid bacterium according to
The present invention described in claim 3 is a method for preparing silage, characterized in that the microorganism preparation described in claim 2 is added to the silage material grass.
The present invention according to claim 4 is the method according to claim 3 , wherein the silage material grass is feed rice.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in detail.
The lactic acid bacteria of
Lactic acid bacteria were isolated from forage crops cultivated by dairy farmers in Tochigi Prefecture. That is, 10 g of a fresh sample of forage crop was collected in a plastic bag for stomacher (Hiryu KN208, manufactured by Asahi Kasei Co., Ltd.) and 90 ml of sterilized physiological saline was added to make a 10-fold diluted solution. Dilute to 8 times.
This diluted solution is applied to Lactobacilli MRS agar medium (DIFCO Laboratories, Detroit, USA) and GYP white agar medium (Ozaki et al., “Lactic acid bacteria experiment manual”, 1992), which is a medium for lactic acid bacteria. )) For 2 days at 37 ° C.
The lactic acid strains isolated from the culture were examined for mycological properties, physiological / biochemical properties, etc., further subjected to a silage preparation suitability test, and the above lactic acid strains were selected.
[0008]
Lactobacillus plantarum No. 1 strain is a homofermentative lactic acid bacterium that is particularly excellent in low pH tolerance and lactic acid-producing ability.
Although this strain has the closest relationship with the reference strain, it differs from the reference strain in terms of sugar fermentation characteristics and antibacterial action, so the inventors have determined that it is a new strain and named as described above. did. This strain is deposited at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, and its deposit number is FERM P-18930 .
[0009]
The present invention according to claim 2 is a microbial preparation for silage preparation containing the lactic acid bacteria .
Next, the silage preparation method according to the third aspect of the present invention will be described.
Forage rice cultivated by farmers in Tochigi and Saitama prefectures (variety: hamasari, kusahonami) was tested, and using the roll bale silage preparation system, the lactic acid bacteria were added to prepare rice fermentation roughage, and silage fermentation The status of the process and the quality of the feed were investigated, and it was confirmed that this lactic acid bacterium was a suitable microorganism for silage preparation. In addition, as the silage material grass, as described in claim 4 , feed rice is preferable.
[0010]
According to the silage preparation method of the present invention, not only heterofermentative lactic acid bacteria derived from the material grass but fermentation by homofermentative lactic acid bacteria is performed, so that not only the quality of fermented feed is improved, but also the amount of silage gas produced And the dry matter loss rate decreases significantly. This suppression of fermentation loss is a result of vigorous lactic acid fermentation by shortening the active period of other harmful microorganisms by adding specific lactic acid bacteria.
Lactobacillus plantarum stock No. 1 is a lactobacillus, grows over a long period of the silage fermentation process, and rapidly lowers the silage pH by vigorous lactic acid fermentation.
Such large- scale production of lactic acid can suppress the growth of butyric acid bacteria, aerobic bacteria, and the like, which are defective microorganisms for silage fermentation, and can efficiently improve the quality of silage fermentation.
[0011]
【Example】
Hereinafter, the present invention will be described in detail by way of examples, but the present invention is not limited thereto.
Example 1
After collecting 10g of a fresh sample of forage crop cultivated by a dairy farmer in Tochigi Prefecture in a plastic bag for stomacher (Hiryu KN208, manufactured by Asahi Kasei Co., Ltd.) and adding 90 ml of sterilized physiological saline to make a 10-fold dilution This solution was further diluted to 10 8 times.
This diluted solution is applied to Lactbacilli MRS agar medium (DIFCO Laboratories, Detroit, USA) and GYP white agar medium (Osaki et al., “Lactic acid bacteria experiment manual”, 1992), which is a medium for lactic acid bacteria. )) For 2 days at 37 ° C.
Lactic acid strains isolated from the culture were tested for bacterial morphology observation, Gram staining, spore formation, catalase reaction and lactic acid production.
Furthermore, physiological and biochemical properties, saccharolytic properties, and silage preparation suitability tests of these isolates were performed. Based on these results, it was possible to select a Lactobacillus plantarum No. 1 strain which was a homofermentative lactic acid bacterium and was most excellent in low pH tolerance and lactic acid-producing ability.
Table 1 shows the physiological and biochemical properties of the lactic acid bacteria .
[0012]
[Table 1]
Table 1
* Livestock No.1: Lactobacillus plantarum No.1 [0013]
As is clear from Table 1, Lactobacillus plantarum No. 1 strain is gram-negative, catalase-negative, homo-fermentation that does not produce gas from glucose, and mainly produces lactate isomers of DL-type and L-type It is a lactic acid bacterium.
[0014]
From these, it can be seen that the lactic acid bacteria according to the present invention are suitable as a microbial preparation for silage preparation.
Next, Lactobacillus plantarum stock No. 1 was identified. First, the taxonomic position of this lactic acid bacterium is shown in FIG .
[0015]
The base sequence of the 16S rRNA gene in the entire region of this strain was determined and compared with other bacterial species. As a result, the position of the molecular system of the No. 1 stock was found in the Lactobacillus cluster .
Lactobacillus plantarum畜草No. 1 strain is in the most closely related phylogenetic relationships between the type strain of Lactobacillus plantarum, to show 99% or more of 16S rRNA sequence homology to each other, which was identified as described above.
Moreover, as shown in Table 2 , the saccharide fermentation characteristics of this strain are different from those of the reference strain. That is, Lactobacillus plantarum No. 1 strain is different from the reference strain in the fermentation characteristics of rhamnose, dulcitol, α-methyl-D-glucoside and esculin .
From these, this strain was determined to be a new strain . This strain is deposited at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, and its deposit number is FERM P-18930 .
[0016]
[Table 2]
Table 2 (Part 1)
[Table 3]
Table 2 (Part 2)
Example 2
Forage rice cultivated by farmers in Tochigi Prefecture and Saitama Prefecture (variety: hamasari, kusahonami) was tested and a rice fermented roughage was prepared using a roll bale system for feed rice harvest preparation. The lactic acid bacteria for addition were inoculated into a Lactobacilli MRS liquid medium (DIFCO Laboratories, Detroit, USA), cultured at 30 ° C. for 16 hours under anaerobic conditions, and then the bacterial culture was adjusted to 1.0 × 10 8 cfu / ml. 1 ml per 1 kg of feed rice was uniformly added by an automatic addition device attached to a roll baler.
These roll bale silages were left outdoors (temperature: 19-35 ° C.), and after 176 days after storage, the roll bale silages were disassembled and the fermentation quality was analyzed. Table 3 shows the microbial species composition (176 days after storage) of the rice fermented roughage, and Table 4 shows the fermentation quality of roll bale silage.
[0019]
[Table 4]
Table 3
*: Same as Table 1 [0020]
[Table 5]
Table 4
As apparent from Table 3, the silage prepared by adding the lactic acid bacterium according to the present invention has a significantly increased number of lactic acid bacteria in the silage fermentation process compared to the silage without the addition of the lactic acid bacterium. However, the number of bacteria such as butyric acid bacteria and Bacillus bacteria decreased rapidly. The reason why the growth of harmful microorganisms is suppressed is the decrease in pH due to the production of a large amount of lactic acid. That is, a high initial number of bacteria by Lactic Acid Bacteria also becomes superior in competition with other microorganisms, yet the strong lactic acid production ability of homofermentative lactic acid bacteria added, such as by lowering the pH quickly, other defective microbes Proliferation was effectively suppressed.
Furthermore, it has been found that the lactic acid bacteria according to the present invention show good growth in a silage environment and suppress the growth of harmful microorganisms such as butyric acid bacteria and Escherichia coli during the silage fermentation process.
[0022]
Also, from Table 4 , the silage prepared by adding the lactic acid bacterium according to the present invention has a lower pH value, the amount of acetic acid and butyric acid and the amount of ammonia nitrogen, compared to the silage without the addition of lactic acid bacterium. The production amount is increased, a high-quality silage is obtained, and the silage fermentation loss is significantly reduced.
The improvement in silage fermentation quality can be attributed to the use of the homofermentative lactic acid bacteria, not the heterofermentative lactic acid bacteria derived from the material grass.
[0023]
【The invention's effect】
The present invention provides a novel lactic acid bacterium useful for suppressing the growth of harmful microorganisms and promoting the growth of preferable lactic acid bacteria. That is, high lactic acid fermentation in the environment of silage fermentation, homofermentative lactic acid bacteria to suppress the growth of harmful microorganisms such as aerobic bacteria and butyric acid bacteria by the production of homofermentative lactic acid bacteria and antibacterials low pH tolerance is provided which Microbial preparations containing are useful for silage preparation.
Furthermore, the present invention provides a method for preparing high-quality silage by adding the microorganism preparation to silage material grass such as feed rice.
Therefore, according to the present invention, it is possible to effectively use idle paddy fields and improve the feed self-sufficiency rate.
[Brief description of the drawings]
FIG. 1 is a molecular phylogenetic tree of 16S rRNA genes of Lactobacillus plantarum No. 1 strain and closely related lactic acid bacteria (created by NJ method using evolution distance determined by Knuc) .
Claims (4)
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| CN103781898A (en) * | 2011-06-29 | 2014-05-07 | 雪印种苗株式会社 | Novel lactic acid bacterium and method for preparing silage or fermented feed by using the same |
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| JP4989056B2 (en) * | 2005-09-22 | 2012-08-01 | 独立行政法人農業・食品産業技術総合研究機構 | Microbial preparations for feed preparation and their use |
| ATE500742T1 (en) * | 2006-07-14 | 2011-03-15 | Legarth Lone | HOMOFERMENTED PRODUCTS |
| CN102864093B (en) * | 2012-04-18 | 2014-01-15 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum suitable for silage straw and use method thereof |
| EE05746B1 (en) * | 2013-07-30 | 2015-05-15 | OÜ Tervisliku Piima Biotehnoloogiate Arenduskeskus | Microorganism Lactobacillus plantarum TAK 59 NCIMB42150 and use thereof |
| JP6455991B2 (en) * | 2015-04-15 | 2019-01-23 | 株式会社和農産 | Breeding of beef cattle |
| JP6807160B2 (en) | 2016-03-15 | 2021-01-06 | 雪印種苗株式会社 | Lactic acid bacteria for silage preparation and additives for silage preparation |
| CN107927353B (en) * | 2017-10-30 | 2021-03-23 | 中国科学院生态环境研究中心 | Lactobacillus inoculant suitable for ensiling of myriophyllum viridis as well as preparation method and application thereof |
| JP7062443B2 (en) * | 2018-01-04 | 2022-05-06 | 雪印種苗株式会社 | Lactic acid bacteria preparation for silage preparation |
| CN113755383B (en) * | 2021-09-27 | 2023-10-03 | 华南农业大学 | Low-nutrition-resistant lactic acid bacteria and application thereof |
| CN113736710B (en) * | 2021-09-27 | 2024-02-09 | 华南农业大学 | Oxygen-resistant lactic acid bacteria and application thereof |
| CN115011515B (en) * | 2022-06-10 | 2023-07-04 | 黑龙江八一农垦大学 | Strain for increasing plant alcohol content in alfalfa silage and application thereof |
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