JP3534295B2 - Mushroom artificial culture medium and mushroom artificial cultivation method using the same - Google Patents
Mushroom artificial culture medium and mushroom artificial cultivation method using the sameInfo
- Publication number
- JP3534295B2 JP3534295B2 JP35873297A JP35873297A JP3534295B2 JP 3534295 B2 JP3534295 B2 JP 3534295B2 JP 35873297 A JP35873297 A JP 35873297A JP 35873297 A JP35873297 A JP 35873297A JP 3534295 B2 JP3534295 B2 JP 3534295B2
- Authority
- JP
- Japan
- Prior art keywords
- culture medium
- weight
- cas
- artificial culture
- sulfate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Mushroom Cultivation (AREA)
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、きのこの人工培養
基及びそれを用いたきのこの人工栽培方法に関する。The present invention relates to an artificial culture medium for mushrooms and a method for artificially cultivating mushrooms using the same.
【０００２】[0002]
【従来の技術とその課題】従来、きのこの栽培は、くぬ
ぎ、ぶな、及びなら等の原木を利用した、ほだ木栽培が
ほとんどであり、そのため、気象条件により収穫が左右
されることが多いという課題があった。また、最近で
は、ほだ木栽培用の原木切り出しのための労働力が不足
していることなどによって原木の入手が困難になりつつ
ある。さらに、ほだ木栽培では栽培期間が長いこと、即
ち、種菌の接種からきのこの収穫までに１年半〜２年も
要することにより、生産コストが相当高くつくのが実情
である。2. Description of the Related Art Conventionally, mushroom cultivation has been mostly cultivation of sodwood using raw wood such as nuts, bunches, nara, and the like. Therefore, harvesting often depends on weather conditions. There was a problem. Recently, it is becoming difficult to obtain raw wood due to a shortage of labor for cutting wood for cultivating soda wood. Furthermore, in the cultivation of sodwood, since the cultivation period is long, that is, it takes one and a half to two years from inoculation of the inoculum to harvesting of the mushroom, the fact is that the production cost is considerably high.
【０００３】近年、えのきたけ、ひらたけ、なめこ、及
びしいたけ等は、鋸屑に米糠を配合した培養基を用い、
瓶又は箱で栽培を行う菌床人工栽培方法が確立され、一
年を通じて、四季に関係なく安定してこれらのきのこが
収穫できるようになっている。即ち、農家での副業的性
格が強く、小規模生産に頼っていたきのこ栽培が、現在
では大規模専業生産が可能で、かつ、原料が入手しやす
い菌床人工栽培方法に移りつつある。しかしながら、菌
床人工栽培方法においても、きのこを大量に連続栽培す
るには、いまだ収率も低く、かつ、栽培期間がかなり長
いため、その生産コストは安価とはいえず、今後これら
生産性の改善が切望されている。例えば、(Al2O3) X (S
iO2)（ただし、式中のx は１以上の数）で示される化合
物を上記の人工培養基に含有させたものや、(MgO) W (A
l2O3) X (SiO2)y （ただし、式中のw は１〜３の数、x
は１〜５の数、y は０〜３の数）で示される化合物を上
記の人工培養基に含有させたものがあるが、充分な収率
できのこを生産することができていないのが現状である
（特開平 3−210126号公報、特開平 3− 58716号公
報）。In recent years, enokitake, hiratake, nameko, shiitake, and the like use a culture medium in which rice bran is mixed with sawdust,
A method of artificially cultivating a fungus bed in which cultivation is carried out in a bottle or a box has been established, and it has become possible to stably harvest these mushrooms throughout the year regardless of the four seasons. In other words, mushroom cultivation, which has relied on small-scale production because of the sideline nature of farmers, is now shifting to a method of artificially cultivating fungal beds, which enables large-scale specialized production and allows easy access to raw materials. However, even in the fungal bed artificial cultivation method, in order to continuously cultivate a large amount of mushrooms, the yield is still low and the cultivation period is considerably long, so that the production cost is not inexpensive. Improvement is eagerly awaited. For example, (Al 2 O 3 ) X (S
iO 2 ) (where x in the formula is 1 or more) in which the compound represented by the above-mentioned artificial culture medium is contained, or (MgO) W (A
l 2 O 3 ) X (SiO 2 ) y (where w is a number of 1 to 3, x
Is a number of 1 to 5, y is a number of 0 to 3). The above-mentioned artificial culture medium contains a compound represented by the formula (1). However, at present, mushrooms cannot be produced at a sufficient yield. (JP-A-3-210126, JP-A-3-58716).
【０００４】本発明者は、きのこの人工栽培における従
来方法の課題を解決するため、誠意検討を重ねた結果、
特定の人工培養基を使用することにより、きのこを高収
率で栽培できることを見いだし、本発明を完成するに至
った。本発明の目的は高収率できのこを人工栽培する方
法を提供することにある。The present inventor has conducted sincere studies to solve the problems of the conventional methods for artificial mushroom cultivation.
The present inventors have found that mushrooms can be grown at a high yield by using a specific artificial culture medium, and have completed the present invention. An object of the present invention is to provide a method for artificially cultivating mushrooms with high yield.
【０００５】[0005]
【課題を解決するための手段】即ち、本発明は、Al ２ O
３含有率が 35 〜 95 重量％のカルシウムアルミネート類又
はAl ２ O ３含有率が 35 〜 95 重量％のカルシウムアルミネ
ート類と硫酸塩を含有してなるきのこの人工培養基であ
り、該人工培養基を用いてなるきのこの人工栽培方法で
ある。That is, the present invention relates to Al 2 O
3 is a calcium aluminate having a content of 35 to 95 % by weight or a calcium aluminate having an Al 2 O 3 content of 35 to 95 % by weight and a sulfate, and is an artificial culture medium of mushrooms. This is an artificial cultivation method using mushrooms.
【０００６】以下、本発明をさらに詳しく説明する。Hereinafter, the present invention will be described in more detail.
【０００７】本発明で使用するカルシウムアルミネート
類（以下ＣＡ類という）とは、きのこの収率を向上する
ために必要なもので、生石灰(CaO) 、消石灰(Ca(OH)2)
、及び石灰石(CaCO3) 等のカルシア原料や、アルミ
ナ、ボーキサイト、ダイアスポア、長石、及び粘土等の
アルミナ原料などを所定の割合で配合した後、ロータリ
ーキルン、直接通電炉、及び高周波炉等を用いて製造さ
れる。ＣＡ類の具体的な化合物としては、3CaO・Al2O3
(C3A) 、12CaO ・7Al2O3 (C1 2A7)、5CaO・3Al2O3 (C
5A3) 、CaO ・Al2O3 (CA)、3CaO・5Al2O3 (C3A5) 、CaO
・2Al2O3 (CA2)、及びCaO ・6Al2O3 (CA6)等があるが、
特に、溶融物を急冷して得られる非晶質のカルシウムア
ルミネートの使用がより好ましい。ＣＡ類中のCaO 含有
率は５〜65重量％、Al2O3 含有率は35〜95重量％が好ま
しく、CaO 含有率は20〜50重量％、Al2O3 含有率は50〜
80重量％がより好ましい。CaO 含有率がこの範囲外で
は、きのこの収率が向上しない場合がある。さらに、カ
ルシウムアルミネートに、その他の成分として、ナトリ
ウム、カリウム、及びリチウム等のアルカリ金属を含有
した化合物（8CaO・Na2O・3Al2O3や3CaO・2Na2O ・5Al2
O3など）又は固溶体やガラス、あるいは、カルシウムア
ルミネートに、マグネシウム、ストロンチウム、及びバ
リウム等のアルカリ土類金属を含有した、化合物（3CaO
・MgO ・2Al2O3や2CaO・2BaO・4Al2O3など）又は固溶体
やガラス、あるいは、カルシウムアルミネートに、ホウ
素、フッ素、ケイ素、リン、イオウ、及び塩素等の非金
属元素を含有した、化合物（11CaO ・7Al2O3・CaF2、3C
aO・3Al2O3・CaF2、2CaO・Al2O3 ・SiO2、及び4CaO・3A
l2O3・SO3 など）又は固溶体やガラス、あるいは、カル
シウムアルミネートに、チタン、クロム、マンガン、
鉄、コバルト、ニッケル、銅、亜鉛、ガリウム、及びゲ
ルマニウム等の重金属を含有した、化合物（4CaO・Al2O
3 ・Fe2O3 など）又は固溶体やガラス、さらに、カルシ
ウムアルミネートに、アルカリ金属、アルカリ土類金
属、非金属元素、及び重金属から選ばれた二種以上を含
有した、化合物又は固溶体やガラス等も、本発明におけ
るCaO 又はAl2O3 含有率の範囲内であれば使用すること
ができる。ＣＡ類の粒度は、少量の添加量できのこの収
率が向上することから小さいほど好ましい。具体的に
は、１mm以下が好ましく、100 μｍ以下がより好まし
い。ＣＡ類の使用量は、人工培養基 100重量部中、0.01
〜30重量％が好ましく、0.1 〜10重量％がより好まし
い。この範囲外ではきのこの収率の向上がみられない場
合がある。本発明のＣＡ類としては各種アルミナセメン
トの使用も可能である。[0007] Calcium aluminates (hereinafter referred to as CAs) used in the present invention are necessary for improving the yield of mushrooms, and include quick lime (CaO) and slaked lime (Ca (OH) 2 ).
And calcite raw materials such as limestone (CaCO 3 ) and alumina raw materials such as alumina, bauxite, diaspore, feldspar, and clay at a predetermined ratio, and then mixed with a rotary kiln, a direct current furnace, and a high-frequency furnace. Manufactured. Specific compounds of CAs include 3CaO.Al 2 O 3
(C 3 A), 12CaO · 7Al 2 O 3 (C 1 2 A 7), 5CaO · 3Al 2 O 3 (C
5 A 3), CaO · Al 2 O 3 (CA), 3CaO · 5Al 2 O 3 (C 3 A 5), CaO
・ 2Al 2 O 3 (CA 2 ) and CaO ・ 6Al 2 O 3 (CA 6 )
In particular, the use of amorphous calcium aluminate obtained by quenching the melt is more preferable. CaO content in the CA compound is 5 to 65 wt%, Al 2 O 3 content is preferably 35 to 95 wt%, the CaO content is 20 to 50 wt%, the Al 2 O 3 content 50
80% by weight is more preferred. If the CaO content is outside this range, the mushroom yield may not be improved. Furthermore, the calcium aluminate, as other components, sodium, potassium, and compounds containing an alkali metal such as lithium (8CaO · Na 2 O · 3Al 2 O 3 and 3CaO · 2Na 2 O · 5Al 2
O 3, etc.), or solid solution, glass or, calcium aluminate, magnesium, strontium, and containing an alkaline earth metal such as barium, compound (3CaO
・ MgO ・ 2Al 2 O 3 or 2CaO ・ 2BaO ・ 4Al 2 O 3 ) or solid solution or glass, or calcium aluminate contained non-metallic elements such as boron, fluorine, silicon, phosphorus, sulfur, and chlorine compound (11CaO · 7Al 2 O 3 · CaF 2, 3C
aO ・ 3Al 2 O 3・ CaF 2 , 2CaO ・ Al 2 O 3・ SiO 2 , and 4CaO ・ 3A
l 2 O 3 · SO 3 ) or solid solution or glass, or calcium aluminate, titanium, chromium, manganese,
Compounds containing heavy metals such as iron, cobalt, nickel, copper, zinc, gallium, and germanium (4CaO.Al 2 O
3・ Fe 2 O 3 ) or a solid solution or glass, or a compound or a solid solution or glass containing calcium aluminate containing two or more selected from alkali metals, alkaline earth metals, nonmetal elements, and heavy metals Etc. can be used within the range of the content of CaO or Al 2 O 3 in the present invention. The smaller the particle size of the CAs is, the smaller the addition amount is, because the yield is improved. Specifically, it is preferably 1 mm or less, more preferably 100 μm or less. The amount of CAs used is 0.01% in 100 parts by weight of the artificial culture medium.
-30% by weight is preferred, and 0.1-10% by weight is more preferred. Outside this range, the yield of mushrooms may not be improved. As the CAs of the present invention, various alumina cements can be used.
【０００８】本発明において、ＣＡ類に硫酸塩を併用す
ることにより、きのこの収率がさらに向上する。硫酸塩
としては、無水セッコウ、半水セッコウ、二水セッコ
ウ、無水硫酸アルミニウム、６、10、16、及び18水塩等
の含水硫酸アルミニウム、無水硫酸ナトリウム、７や10
水塩などの含水硫酸ナトリウム、無水硫酸マグネシウ
ム、１、６、及び７水塩等の含水硫酸マグネシウム、無
水硫酸リチウム、並びに、硫酸リチウム１水塩等が好ま
しく、特に、無水セッコウが好ましい。硫酸塩の粒度
は、わずかの添加量できのこの収率が向上することから
小さいほど好ましい。具体的には、１mm以下が好まし
く、100 μｍ以下がより好ましい。硫酸塩の使用量は、
人工培養基 100重量部中、0.01〜30重量％が好ましく、
0.1 〜10重量％がより好ましい。In the present invention, the combined use of sulfates with CAs further improves the yield of mushrooms. Examples of the sulfate include anhydrous gypsum, hemihydrate gypsum, dihydrate gypsum, anhydrous aluminum sulfate, hydrated aluminum sulfate such as 6, 10, 16 and 18 hydrates, anhydrous sodium sulfate, 7 and 10
Hydrous sodium sulfate such as water salt, anhydrous magnesium sulfate, anhydrous magnesium sulfate such as 1, 6, and heptahydrate, anhydrous lithium sulfate, lithium sulfate monohydrate and the like are preferable, and anhydrous gypsum is particularly preferable. The smaller the particle size of the sulfate is, the smaller the amount is. Specifically, it is preferably 1 mm or less, more preferably 100 μm or less. The amount of sulfate used is
In 100 parts by weight of the artificial culture medium, 0.01 to 30% by weight is preferable,
0.1 to 10% by weight is more preferred.
【０００９】本発明で使用する人工培養基としては、鋸
屑、もみ殻、コーンコブ、バガス、パルプ廃材、ビート
粕、及びデンプン粕等の基材に、米ぬか、もろこし粉砕
物、及びフスマ等の栄養源の一種又は二種以上を混合し
たものを使用する。通常、乾燥基材１重量部に対して、
栄養源約 0.1〜1.5 重量部を混合したものを用いる。き
のこの種類、栽培環境や条件等に応じて、基材や栄養源
の種類、両者の配合割合は任意に変化するもので、特に
限定されるものではないが、鋸屑 100重量部に対して、
栄養源の少なくとも一種を10〜150 重量部混合したもの
が、きのこを高収穫量得る面からより好ましい。[0009] The artificial culture medium used in the present invention includes a substrate such as sawdust, rice husk, corn cob, bagasse, pulp waste material, beet cake and starch cake, and a nutrient source such as rice bran, mashed mash, and bran. Use one or a mixture of two or more. Usually, for 1 part by weight of the dry base material,
Use a mixture of about 0.1 to 1.5 parts by weight of nutrient sources. Depending on the type of mushroom, cultivation environment and conditions, the type of base material and nutrient source, the mixing ratio of both are arbitrarily changed, and it is not particularly limited, but for 100 parts by weight of sawdust,
A mixture of 10 to 150 parts by weight of at least one nutrient is more preferable from the viewpoint of obtaining a high yield of mushrooms.
【００１０】本発明により、きのこを栽培するにあたっ
ては、各々の環境や状況などに応じて任意に変えること
ができるので特に限定されるものではないが、通常、Ｃ
Ａ類又はＣＡ類と硫酸塩を混合した人工培養基に水を加
えて、人工培養基の水分含有量を50〜70重量％に調整
し、必要に応じて殺菌・冷却後、菌を接種し、各々のき
のこについて通常採用されている培養工程や生育条件に
従って行うとよい。例えば、ほんしめじ栽培の場合は、
菌を接種した培養基を22〜26℃で約30日間培養後、25〜
30℃で40〜50日間熟成し、菌かき後に後温度14〜17℃、
湿度95〜100 ％で20〜25日間育成を行って、ほんしめじ
を栽培し収穫する。また、しいたけ栽培の場合は、菌を
接種した培養基を20〜25℃で約30日間培養後、26〜30℃
で40〜50日間熟成し、その後温度13〜17℃で１〜３日間
低温処理し、温度17〜20℃、湿度90〜95％で約10日間発
生を行ってきのこを収穫し、この際に第１回目の収穫後
に再び発生にかけて第２回目の収穫を行うこともでき
る。[0010] According to the present invention, the cultivation of mushrooms is not particularly limited because it can be arbitrarily changed according to each environment and situation.
Water is added to the artificial culture medium in which the A or CAs and sulfates are mixed to adjust the water content of the artificial culture medium to 50 to 70% by weight. The mushroom may be performed in accordance with a culture step or growth condition generally used. For example, in the case of cultivation,
After culturing the culture medium inoculated with the bacteria at 22 to 26 ° C for about 30 days,
Aged at 30 ° C for 40-50 days, after scraping the bacteria, the temperature is 14-17 ° C,
The cultivation is carried out at 95-100% humidity for 20-25 days, and the shimeji is grown and harvested. In addition, in the case of shiitake cultivation, the culture medium inoculated with the bacteria is cultured at 20 to 25 ° C for about 30 days, and then 26 to 30 ° C
And then aged at a temperature of 13 to 17 ° C for 1 to 3 days at low temperature. After the first harvest, a second harvest can also be performed on the outbreak again.
【００１１】本発明では、前述の基材や栄養源の他に
も、必要に応じて人工培養基において使用されている、
例えば、炭酸カルシウム、卵殻粉末、貝殻粉末、及び消
石灰等の成分を含むことができる。本発明で栽培される
きのこは人工栽培できるきのこであり、例えば、えのき
たけ、ひらたけ、なめこ、ぶなしめじ、まいたけ、きく
らげ、さるのこしかけ、及びしいたけ等が挙げられる。[0011] In the present invention, in addition to the above-mentioned substrate and nutrient source, if necessary, an artificial culture medium is used.
For example, ingredients such as calcium carbonate, eggshell powder, shell powder, and slaked lime can be included. The mushrooms cultivated in the present invention are mushrooms that can be artificially cultivated, and include, for example, enokitake, open mushroom, nameko, mushroom, mushroom, jellyfish, monkey mushroom, and shiitake mushroom.
【００１２】[0012]
【実施例】以下、本発明の実施例を示し、本発明をさら
に説明するが、本発明はこれらに限定されるものではな
い。The present invention will be further described below with reference to examples of the present invention, but the present invention is not limited to these examples.
【００１３】実施例１
炭酸カルシウムとアルミナを所定の割合で混合し、電気
炉を用い、表１に示す温度で２時間焼成し、表１に示す
ＣＡ類を生成した。生成したＣＡ類を粉砕し、100 μｍ
以下として使用した。広葉樹鋸屑250g、針葉樹鋸屑250
g、米糠500g、及び水 140mlをビニール袋に入れ充分に
混合し水分含水率65％の人工培養基を調製した。調製し
た人工培養基 100重量部中、３重量部となるように表１
に示すＣＡ類を添加混合した人工培養基250gをプラスチ
ック製 850mlの広口瓶に圧詰めした。広口瓶の中央に直
径約１cm程度の穴を開け、打栓後、120 ℃で90分間殺菌
した。冷却後、ひらたけの鋸屑種菌を植菌し、暗所、温
度25℃、湿度55％の条件下で30日間培養し（菌まわし行
程）、さらに、30日間培養を続けて熟成させた。次に、
栓を外して培養基の上部から約１cm程度菌かきをして菌
糸層を除いた後、水道水20mlを添加して充分に吸水させ
た。４時間放置後、上部に残った水を取り除いて、温度
15℃、湿度95％、照度20ルックスの条件下で、４日間培
養して子実体原基を形成させ、さらに照度を200 ルック
スに上げて、10日間培養を続け、ＣＡ類の組成が子実体
収量におよぼす影響について検討した。結果を表１に併
記する。Example 1 Calcium carbonate shown in Table 1 was produced by mixing calcium carbonate and alumina at a predetermined ratio and calcining the mixture in an electric furnace at a temperature shown in Table 1 for 2 hours. The generated CAs are crushed and 100 μm
Used as: Hardwood sawdust 250g, softwood sawdust 250
g, 500 g of rice bran and 140 ml of water were put in a plastic bag and mixed well to prepare an artificial culture medium having a water content of 65%. Table 1 shows that 3 parts by weight of 100 parts by weight of the prepared artificial culture medium was used.
250 g of the artificial culture medium to which the CAs shown in (1) were added and mixed was pressed into a plastic 850 ml wide-mouth bottle. A hole having a diameter of about 1 cm was made in the center of the jar, and after stoppering, sterilized at 120 ° C. for 90 minutes. After cooling, Hiratake sawdust was inoculated and cultured for 30 days in a dark place at a temperature of 25 ° C. and a humidity of 55% (the fungus turning process), followed by culturing for 30 days, followed by ripening. next,
After removing the stopper and scraping about 1 cm from the top of the culture medium to remove the mycelium layer, 20 ml of tap water was added to allow sufficient water absorption. After leaving for 4 hours, remove the water remaining on the top
Cultured at 15 ° C, 95% humidity, 20 lux illuminance for 4 days to form fruiting body primordia, increased the illuminance to 200 lux and continued cultivation for 10 days. The effect on yield was studied. The results are also shown in Table 1.
【００１４】＜使用材料＞ 炭酸カルシウム：試薬、CaCO3 アルミナ ：試薬、Al2O3 広葉樹鋸屑：ぶな材の鋸屑 針葉樹鋸屑：すぎ材の鋸屑 米糠 ：市販品<Materials Used> Calcium carbonate: Reagent, CaCO 3 Alumina: Reagent, Al 2 O 3 Hardwood sawdust: Sawwood conifer Softwood sawdust: Sawwood sawdust Rice bran: Commercial product
【００１５】＜測定方法＞
コントロール対比：（ＣＡ類＋硫酸塩）添加の子実体収
量（ｇ）／（ＣＡ類＋硫酸塩）無添加の子実体収量
（ｇ）×１００ （％）<Measurement method> Control comparison: Fruit body yield (g) with (CAs + sulfate) added / body yield (g) × 100 (%) without (CAs + sulfate) added
【００１６】[0016]
【表１】 [Table 1]
【００１７】表１から明らかなように、人工培養基に、
ＣＡ類を添加することにより、ひらたけの収率が飛躍的
に増大した。As is clear from Table 1, the artificial culture medium
The addition of CAs dramatically increased the yield of Japanese oyster mushrooms.
【００１８】実施例２
表２に示すＣＡ類Ｄを用いたこと以外は実施例１と同様
に行った。結果を表２に併記する。Example 2 Example 2 was repeated except that CAs D shown in Table 2 were used. The results are also shown in Table 2.
【００１９】[0019]
【表２】 [Table 2]
【００２０】表２から明らかなように、人工培養基に、
ＣＡ類の使用量が５重量部の場合、最もひらたけの収率
が向上した。As is clear from Table 2, the artificial culture medium
When the amount of CAs used was 5 parts by weight, the yield of Japanese hiratake was improved most.
【００２１】実施例３
炭酸カルシウムとアルミナに、さらに、炭酸ナトリウ
ム、硫酸カルシウム、又は酸化チタンを混合して、電気
炉を用い、1,400 ℃で２時間焼成し、表３に示すＣＡ類
を生成し、人工培養基 100重量部中、３重量部なるよう
に配合したこと以外は実施例１と同様に行った。結果を
表３に併記する。Example 3 Calcium carbonate and alumina were further mixed with sodium carbonate, calcium sulfate, or titanium oxide, and calcined at 1,400 ° C. for 2 hours in an electric furnace to produce CAs shown in Table 3. The procedure was carried out in the same manner as in Example 1 except that 3 parts by weight was added to 100 parts by weight of the artificial culture medium. The results are also shown in Table 3.
【００２２】＜使用材料＞ 炭酸ナトリウム：試薬、Na2CO3 硫酸カルシウム：試薬、CaSO4 酸化チタン：試薬、TiO2 <Materials Used> Sodium carbonate: reagent, Na 2 CO 3 calcium sulfate: reagent, CaSO 4 titanium oxide: reagent, TiO 2
【００２３】[0023]
【表３】 [Table 3]
【００２４】表３から明らかなように、人工培養基に、
ＣＡ類を添加することにより、ひらたけの収率が飛躍的
に増大した。As is clear from Table 3, the artificial culture medium
The addition of CAs dramatically increased the yield of Japanese oyster mushrooms.
【００２５】実施例４
表４に示すＣＡ類と硫酸塩を用いたこと以外は実施例１
と同様に行った。結果を表４に併記する。Example 4 Example 1 except that the CAs and sulfates shown in Table 4 were used.
The same procedure was followed. The results are shown in Table 4.
【００２６】＜使用材料＞ 硫酸塩ａ ：無水硫酸アルミニウム、試薬１級品 硫酸塩ｂ ：硫酸アルミニウム18水塩、試薬１級品 硫酸塩ｃ ：無水硫酸ナトリウム、試薬１級品 硫酸塩ｄ ：無水硫酸マグネシウム、試薬１級品 硫酸塩ｅ ：二水セッコウ、試薬１級品 硫酸塩ｆ ：無水セッコウ、試薬１級品<Materials used> Sulfate a: anhydrous aluminum sulfate, reagent grade 1 Sulfate b: Aluminum sulfate 18 hydrate, first grade reagent Sulfate c: anhydrous sodium sulfate, reagent first grade Sulfate d: anhydrous magnesium sulfate, reagent first grade Sulfate e: Gypsum dihydrate, first grade reagent Sulfate f: Anhydrite, first grade reagent
【００２７】[0027]
【表４】 [Table 4]
【００２８】表４から明らかなように、人工培養基に、
ＣＡ類と硫酸塩を併用することにより、ひらたけの収率
が飛躍的に増大した。As is clear from Table 4, the artificial culture medium was:
The combined use of CAs and sulfates dramatically increased the yield of Japanese mustard.
【００２９】実施例５
広葉樹鋸屑350g、針葉樹鋸屑350g、米糠300g、及び水 1
35mlをビニール袋に入れ充分に混合し、水分含水率63％
の人工培養基を調製した。調製した人工培養基250gに、
表５に示すＣＡ類と硫酸塩を添加混合し、プラスチック
製 850ml広口瓶に圧詰めした。各々の中央に直径約１cm
程度の穴を開け、打栓後、120 ℃で90分間殺菌した。冷
却後、ほんしめじの種菌を植菌し、温度23℃にて30日間
培養後、さらに、26℃にて45日間熟成を行った。次に、
菌かきをした後、温度15℃、湿度95％の条件下で生育を
行い、21日後にほんしめじを収穫した。結果を表５に示
す。EXAMPLE 5 350 g of hardwood sawdust, 350 g of softwood sawdust, 300 g of rice bran, and water 1
Put 35ml in a plastic bag, mix well, moisture content 63%
Was prepared. To 250 g of the prepared artificial culture medium,
The CAs and sulfates shown in Table 5 were added, mixed, and pressed into a plastic 850 ml wide-mouth bottle. About 1cm in diameter at the center of each
After making a hole of about a degree and stoppering, sterilization was performed at 120 ° C. for 90 minutes. After cooling, the inoculated fungus was inoculated, cultured at a temperature of 23 ° C. for 30 days, and then aged at 26 ° C. for 45 days. next,
After the fungi were scraped, the plants were grown under the conditions of a temperature of 15 ° C. and a humidity of 95%. Table 5 shows the results.
【００３０】[0030]
【表５】 [Table 5]
【００３１】表５から明らかなように、人工培養基に、
ＣＡ類又はＣＡ類と硫酸塩を使用することにより、ほん
しめじの収率が飛躍的に増大した。As is clear from Table 5, the artificial culture medium
The use of CAs or CAs and sulfates dramatically increased the yield of shimejimoji.
【００３２】実施例６
広葉樹鋸屑300g、針葉樹鋸屑300g、米糠 40g、フスマ 6
0g、もろこし粉砕物 30g、及び水 400mlをビニール袋に
入れ充分に混合し、水分含水率65％の人工培養基を調製
した。調製した人工培養基250gに、表６に示すＣＡ類と
硫酸塩ｆを添加混合し、プラスチック製 850ml広口瓶に
圧詰めした。中央に直径約１cm程度の穴を開け、打栓
後、120 ℃で90分間殺菌した。冷却後、しいたけの種菌
を植菌し、温度23℃にて30日間培養後、さらに、30℃に
て50日間熟成を行った。その後、15℃で２日間低温処理
した後、温度18℃、湿度95％にて育成を行った。10日間
で収穫を行った後、再び生育を行い、２回目の収穫を行
い、合計量をしいたけの子実体収量とした。結果を表６
に示す。Example 6 Hardwood sawdust 300 g, softwood sawdust 300 g, rice bran 40 g, bran 6
0 g, 30 g of crushed moromi and 400 ml of water were put in a plastic bag and mixed well to prepare an artificial culture medium having a water content of 65%. To 250 g of the prepared artificial culture medium, CAs and sulfate f shown in Table 6 were added and mixed, and the mixture was pressed into a plastic 850 ml wide-mouth bottle. A hole having a diameter of about 1 cm was made in the center, and after stoppering, the mixture was sterilized at 120 ° C. for 90 minutes. After cooling, an inoculum of Shiitake was inoculated, cultured at a temperature of 23 ° C. for 30 days, and then aged at 30 ° C. for 50 days. Then, after a low-temperature treatment at 15 ° C. for 2 days, growth was performed at a temperature of 18 ° C. and a humidity of 95%. After harvesting for 10 days, the plants were grown again and harvested for the second time, and the total amount was defined as the yield of shiitake fruit body. Table 6 shows the results
Shown in
【００３３】＜使用材料＞
ＣＡ類Ｌ ：Al2O3 54.3重量％、CaO 36.8重量％、SiO2
3.7重量％、Fe2O31.4重量％、及びTiO2 3.5重量％、合
計99.7重量％のアルミナセメント
ＣＡ類Ｍ ：Al2O3 48.2重量％、CaO 33.0重量％、SiO2
4.7重量％、Fe2O3 10.8重量％、及びTiO2 2.6重量％、
合計99.3重量％のアルミナセメント<Materials used> CAs L: 54.3% by weight of Al 2 O 3 , 36.8% by weight of CaO, SiO 2
3.7% by weight, 1.4% by weight of Fe 2 O 3 and 3.5% by weight of TiO 2 , a total of 99.7% by weight of alumina cement CAs M: 48.2% by weight of Al 2 O 3 , 33.0% by weight of CaO, SiO 2
4.7% by weight, Fe 2 O 3 10.8% by weight, and TiO 2 2.6% by weight,
99.3% by weight of alumina cement
【００３４】[0034]
【表６】 [Table 6]
【００３５】表６から明らかなように、人工培養基に、
ＣＡ類又はＣＡ類と硫酸塩を使用することにより、しい
たけの収率が飛躍的に増大した。As is clear from Table 6, the artificial culture medium
The use of CAs or CAs and sulfates dramatically increased the yield of shiitake.
【００３６】[0036]
【発明の効果】以上、詳細に説明したとおり、本発明に
よる栽培方法によれば、きのこを高収率で得ることが可
能となった。As described above, according to the cultivation method of the present invention, mushrooms can be obtained in high yield.
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.7，ＤＢ名) A01G 1/04 ──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int.Cl. 7 , DB name) A01G 1/04
Claims (3)
ウムアルミネート類を含有してなるきのこの人工培養
基。1. An artificial culture of mushrooms comprising calcium aluminates having an Al 2 O 3 content of 35 to 95 % by weight .
ウムアルミネート類と硫酸塩とを含有してなるきのこの
人工培養基。2. An artificial culture of mushrooms comprising calcium aluminates having an Al 2 O 3 content of 35 to 95 % by weight and sulfates.
てなるきのこの人工栽培方法。3. An artificial cultivation method of a mushroom using the artificial culture medium according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP35873297A JP3534295B2 (en) | 1997-12-26 | 1997-12-26 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP35873297A JP3534295B2 (en) | 1997-12-26 | 1997-12-26 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH11187762A JPH11187762A (en) | 1999-07-13 |
| JP3534295B2 true JP3534295B2 (en) | 2004-06-07 |
Family
ID=18460833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP35873297A Expired - Lifetime JP3534295B2 (en) | 1997-12-26 | 1997-12-26 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3534295B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011109995A (en) * | 2009-11-30 | 2011-06-09 | Denki Kagaku Kogyo Kk | Add-in material for mushroom cultivation, artificial culture medium for mushroom, and method for artificially cultivating mushroom using the culture medium |
| JP2011109996A (en) * | 2009-11-30 | 2011-06-09 | Denki Kagaku Kogyo Kk | Add-in material for mushroom cultivation, artificial culture medium for mushroom, and method for artificially cultivating mushroom using the culture medium |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100368904B1 (en) * | 2000-06-12 | 2003-01-24 | 김재헌 | Chemical supplements medium for culture of pleurotus mushroom |
| JP4578037B2 (en) * | 2001-09-04 | 2010-11-10 | 電気化学工業株式会社 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
| JP4570295B2 (en) * | 2001-09-04 | 2010-10-27 | 電気化学工業株式会社 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
| JP4570296B2 (en) * | 2001-09-04 | 2010-10-27 | 電気化学工業株式会社 | Mushroom artificial culture medium and mushroom artificial cultivation method using the same |
| CN104429624A (en) * | 2015-01-03 | 2015-03-25 | 邬方成 | Oyster mushroom cultivation method |
-
1997
- 1997-12-26 JP JP35873297A patent/JP3534295B2/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011109995A (en) * | 2009-11-30 | 2011-06-09 | Denki Kagaku Kogyo Kk | Add-in material for mushroom cultivation, artificial culture medium for mushroom, and method for artificially cultivating mushroom using the culture medium |
| JP2011109996A (en) * | 2009-11-30 | 2011-06-09 | Denki Kagaku Kogyo Kk | Add-in material for mushroom cultivation, artificial culture medium for mushroom, and method for artificially cultivating mushroom using the culture medium |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11187762A (en) | 1999-07-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3534295B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| WO2010058425A2 (en) | Soil conditioner | |
| JP7055025B2 (en) | Additives for mushroom cultivation, artificial culture groups for mushroom cultivation, medium for mushroom cultivation and artificial cultivation method for mushrooms | |
| JP5527803B2 (en) | Mushroom cultivation additive, mushroom artificial culture medium, and mushroom artificial cultivation method using the same | |
| JP4695907B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP3767998B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP4520568B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP3854093B2 (en) | Hatake shimeji fruit body occurrence rate improver | |
| JP4520616B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP3652877B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP4726171B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP4520587B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP3485775B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP5294270B2 (en) | Mushroom cultivation additive, mushroom artificial culture medium, and mushroom artificial cultivation method using the same | |
| JP2766702B2 (en) | Culture medium for mushroom cultivation | |
| JP4554010B2 (en) | Artificial cultivation method of Maitake | |
| JP3205083B2 (en) | Artificial cultivation method of Hatake shimeji | |
| JP4578036B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP4578035B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP4578037B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP5984255B2 (en) | Mushroom cultivation additive, mushroom artificial culture medium, and mushroom artificial cultivation method using the same | |
| JP4570296B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP4570295B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JP4570297B2 (en) | Mushroom artificial culture medium and mushroom artificial cultivation method using the same | |
| JPH0544247B2 (en) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20040304 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20040304 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080319 Year of fee payment: 4 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090319 Year of fee payment: 5 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100319 Year of fee payment: 6 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110319 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110319 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120319 Year of fee payment: 8 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120319 Year of fee payment: 8 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130319 Year of fee payment: 9 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130319 Year of fee payment: 9 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140319 Year of fee payment: 10 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |