JP3430940B2 - Foods and beverages combining isoflavones and peptides - Google Patents
Foods and beverages combining isoflavones and peptidesInfo
- Publication number
- JP3430940B2 JP3430940B2 JP31354498A JP31354498A JP3430940B2 JP 3430940 B2 JP3430940 B2 JP 3430940B2 JP 31354498 A JP31354498 A JP 31354498A JP 31354498 A JP31354498 A JP 31354498A JP 3430940 B2 JP3430940 B2 JP 3430940B2
- Authority
- JP
- Japan
- Prior art keywords
- isoflavone
- peptide
- bone
- soybean
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
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- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Tea And Coffee (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Jellies, Jams, And Syrups (AREA)
Description
【0001】[0001]
【発明の属する技術分野】本発明は、イソフラボンとペ
プチドを組み合わせた食品及び飲料に関するものであ
る。TECHNICAL FIELD The present invention relates to foods and beverages in which isoflavones and peptides are combined.
【0002】[0002]
【従来の技術】閉経後の女性ホルモン(エストロジェ
ン)の減少による骨からのCa(カルシウム。以下同
様)流出が原因の病気として、骨粗鬆症が問題になって
いる。骨粗鬆症はCaの流出により骨密度が小さくなる
骨疾患の一種で、これを患うと軽い衝撃でも骨折を起こ
しやすくなる。高齢女性の場合は特に回復が遅いため、
一旦骨折を起こすと、最悪の場合は寝たきりになってし
まう恐れがある。そこで最近では骨の主要成分であるC
aの吸収促進、或いは骨からのCa流出抑制等の効果を
持つ機能性素材が開発されている。なかでも体内で女性
ホルモン様の働きをし、閉経後女性のホルモン減少によ
る骨量の減少を防ぐと言われている大豆イソフラボンが
特に注目を集めている。2. Description of the Related Art Osteoporosis has become a problem as a disease caused by a decrease in female hormone (estrogen) after menopause resulting in Ca (calcium. Osteoporosis is a kind of bone disease in which the bone density decreases due to the outflow of Ca. When suffering from this, a bone fracture is likely to occur even with a light impact. Elderly women have a particularly slow recovery,
Once a bone fracture occurs, in the worst case it can be bedridden. So recently, C which is the main component of bone
A functional material having an effect of promoting absorption of a or suppressing Ca outflow from bone has been developed. Of these, soy isoflavone, which is said to act like a female hormone in the body and prevents the loss of bone mass due to hormone loss in postmenopausal women, has been receiving particular attention.
【0003】このような動向に合わせ、骨の強化を謳っ
た飲食品も増加している。例として、
1.骨の主要成分であるCaに着目し、Ca素材のみを
添加した物
2.Caの吸収促進素材(ビタミンDやカゼインホスホ
ペプチド等)を1.に追加した物
3.Caの骨からの流出抑制素材(大豆イソフラボン
等)を1.又は2.に追加した物
4.Caに関するミネラルバランスを整えるため、マグ
ネシウム等のミネラル類を1.ないし3.に追加した物
5.骨形成を促進すると言われているビタミンKを1.
ないし4.に追加した物などのいずれかの区分・観点に
立っての商品開発が見られる。In accordance with such trends, the number of foods and drinks that claim to strengthen bones is increasing. As an example, 1. Focusing on Ca, which is the main component of bone, and adding only Ca material 2. 1. A material that promotes the absorption of Ca (vitamin D, casein phosphopeptide, etc.) Items added to 3. 1. A material that suppresses the outflow of Ca from bone (soy isoflavone, etc.) Or 2. Added to 4. In order to balance the mineral balance related to Ca, 1. Through 3. Items added to 5. Vitamin K, which is said to promote bone formation, 1.
Through 4. Product development can be seen from the perspective of any of the items added to.
【0004】上記の骨強化食品は全て、骨の主成分であ
るCaの吸収促進・流出抑制により骨量を維持し、骨自
体を強化させ、骨粗鬆症等の骨疾患、更には骨折を防ぐ
という考え方に基づいている。しかし、骨粗鬆症、更に
は骨折を防ぐことを目的とした場合、上記の骨強化食品
では以下の点で不充分である。すなわち、
(1)骨自体の強化には、Caの補給・吸収促進・流出
抑制だけでなく、もう一つの主成分であるたん白質の補
給も必要である。
(2)高齢者の場合、骨折の直接原因は80%が転倒に
よるものであり、これは足腰の筋力低下によるものであ
る。従って、骨折の防止には骨自体の強化だけでなく、
骨を包む筋肉を強化することも必要である。
上記の2点を満たすためには、たん白質の補給が必要で
ある。更に、たん白質源としては、たん白質を加水分解
したペプチドの方が、高齢者にも消化・吸収への負担が
少なく、骨や筋肉への吸収が早いため、より好ましい。
すなわち、前記の5つの区分・観点に無い下記の第6.
のものが、上記の(1)、(2)の点からも要望される
背景がある。
6.イソフラボン・ペプチドによる骨自体の強化及びペ
プチド等による骨を包む筋肉の強化を目的とする物、が
望まれるであろう。The above-mentioned bone-reinforced foods all have the idea that bone mass is maintained by promoting absorption and outflow of Ca, which is the main component of bone, and strengthening the bone itself to prevent bone diseases such as osteoporosis and further fracture. Is based on. However, for the purpose of preventing osteoporosis and further bone fracture, the above bone-reinforced food is insufficient in the following points. That is, (1) strengthening the bone itself requires not only supplementation, absorption promotion, and outflow suppression of Ca, but also supplementation of another main component, protein. (2) In the case of elderly people, 80% of the direct causes of bone fractures are due to falls, and this is due to weakness of the muscles of the legs and waist. Therefore, not only strengthening the bone itself, but also preventing fracture
It is also necessary to strengthen the muscles that surround the bone. To meet the above two points, it is necessary to supplement protein. Furthermore, as a protein source, a peptide obtained by hydrolyzing a protein is more preferable because it does not impose a burden on digestion and absorption even in an elderly person and is rapidly absorbed into bone and muscle.
That is, the following 6th category which is not included in the above 5 categories / viewpoints.
There is a background in which the above items are also desired from the above points (1) and (2). 6. It would be desirable to have isoflavone peptides for strengthening the bone itself and for strengthening the muscle that wraps the bone with peptides or the like.
【0005】イソフラボン又はペプチドに関する特許公
開等は、数多く公開されている。主要な先願として、国
際公開WO95/16362号公報では、豆類中のイソ
フラボン化合物の配糖体を麹菌によって分解してアグリ
コンを多量に含むイソフラボン化合物を得ることを謳っ
ている。また、特開平10−114653号公報では、
イソフラボンを主たる有効成分とすることを特徴とする
骨形成促進及び骨塩量減少防止用組成物で骨粗鬆症や骨
折などの治療及び予防に有用な、食品又は医薬品に使用
することができる組成物を得ることを謳っている。一
方、特開平09−216834号公報では、キニノーゲ
ン及び/又はキニノーゲン分解物を有効成分とする骨形
成促進又は骨吸収防止剤でそれらの作用を賦与した飲食
品、医薬品又は資料の提供を謳っている。しかし、これ
らの従来技術は本発明に言うイソフラボンとペプチドを
併含する食品及び飲料、更には課題とは異なるものであ
る。A large number of patent publications relating to isoflavones or peptides have been published. As a major prior application, International Publication WO95 / 16362 discloses that an isoflavone compound containing a large amount of aglycone is obtained by decomposing the glycoside of the isoflavone compound in beans by koji mold. Further, in Japanese Patent Laid-Open No. 10-114653,
A composition for promoting bone formation and preventing a decrease in bone mineral content, characterized in that isoflavone is the main active ingredient, useful for the treatment and prevention of osteoporosis, bone fracture, etc., and a composition that can be used in foods or pharmaceuticals is obtained. Singing thing. On the other hand, Japanese Patent Application Laid-Open No. 09-216834 claims to provide foods, drinks, medicines or materials to which kininogen and / or a degradation product of kininogen is added as an active ingredient to promote the action of bone formation or bone resorption inhibitor. . However, these conventional techniques are different from the above-mentioned foods and beverages containing isoflavone and peptides, and further the problem.
【0006】[0006]
【発明が解決しようとする課題】上記の6.の区分・観
点に立って、イソフラボンとペプチドを併含する食品及
び飲料で骨の強化とその骨を広く取り巻く筋肉の強化に
役立ち得る食品及び飲料の提供が課題である。[Problems to be Solved by the Invention] From the viewpoints and categories described above, it is an object to provide foods and drinks that can help strengthen bones and strengthen muscles that widely surround the bones with foods and drinks that contain isoflavones and peptides.
【0007】[0007]
【課題を解決するための手段】本発明は、(1)イソフラボンを添加し、乾燥重量でイソフラボン
含量がペプチドに対し0.4%以上である食品及び飲
料、 (2)イソフラボンが大豆胚軸、その乾熱加熱品、或い
はその水溶性溶媒抽出物または有機溶媒抽出物から得ら
れたものである上記(1)の食品及び飲料、 (3)ペプチドが分離大豆たん白をプロテアーゼにより
加水分解した大豆ペプチドである上記(1)または
(2)の食品及び飲料、 (4)用いるイソフラボンが乾燥重量でイソフラボン含
量が1重量%以上、用いるペプチド含有物が乾燥重量で
たん白質含量として50重量%以上のものである上記
(1)〜(3)のいずれかに記載の食品、 (5)Ca(カルシウム)含有素材、ミネラル類、ビタ
ミン類、イソフラボン以外のCa吸収促進又はCa溶出
抑制効果を持つ機能性素材を含む上記(1)〜(4)の
いずれかに記載の食品及び飲料、を提供するものであ
る。 According to the present invention, (1) isoflavone is added and isoflavone in dry weight is added.
Foods and drinks containing 0.4% or more of peptides
Fee, (2) isoflavone soybean hypocotyl, the dry heat heating products, walk
Is obtained from its water-soluble solvent extract or organic solvent extract.
(1) The food and beverage as described above, (3) Peptide isolated soybean protein with protease
The above (1) which is a hydrolyzed soybean peptide or
(2) Food and beverage, (4) Isoflavone used contains dry isoflavone.
1% by weight or more, the peptide content used is dry weight
The above protein content is 50% by weight or more
The food according to any one of (1) to (3), (5) Ca (calcium) -containing material, minerals, bitter
Promotes absorption of Ca or elution of Ca other than mines and isoflavones
Of the above (1) to (4), which contains a functional material having a suppressing effect
To provide the food and beverage according to any one of
It
【0008】[0008]
【発明の実施の形態】以下、本発明を詳細に説明する
と、本発明はイソフラボンとペプチドを併含する食品及
び飲料を提供することに関する。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. The present invention relates to providing foods and beverages containing isoflavones and peptides.
【0009】先ず、本発明の用語を説明する。イソフラ
ボンは、イソフラボン類等の有効成分が失われていない
イソフラボン等を含むものであればどの様なものでも良
い。好ましくは、イソフラボン類等が高含有量のものが
良く、とりわけ、大豆胚軸やその加熱処理により風味を
改良したもの又はそれらを水・含水アルコール等の水性
溶媒で抽出したイソフラボン類等が高含有量の大豆胚軸
加工品等が好適である。本発明で言うイソフラボンはダ
イジン、ゲニスチン、ダイゼイン、ゲニステインの合計
量とし、その定量は一定量の供試物をメタノールで還流
抽出し高速液体クロマトグラフィ法にて各成分を定量
し、その合計量を算出する。ペプチドは、たん白質の加
水分解物であるペプチドを含むものであれば、植物性や
動物性の素材のどの様なものでも良い。好ましくは、ペ
プチド類が高含有量のものが良く、とりわけ、大豆たん
白質の加水分解物が好適である。本発明で言うペプチド
の定量は一定量の供試物を公知のケルダール法又はそれ
に準じる方法により窒素を定量し窒素係数(6.25)
を乗じて算出する。健康食品とは、食品(全ての飲食物
を言う。ただし、医薬品及び医薬部外品は、これを含ま
ない。) の内、栄養成分を補給し、又は特別の保健の用
途に適するものとして販売の用に供する食品を言う。First, the terms of the present invention will be explained. Any isoflavone may be used as long as it contains isoflavone and the like in which active ingredients such as isoflavones are not lost. Preferably, a high content of isoflavones and the like is preferable, and in particular, high content of soy hypocotyls and those whose flavor has been improved by heat treatment or isoflavones obtained by extracting them with an aqueous solvent such as water and hydrous alcohol are contained. An amount of processed soybean hypocotyl and the like is suitable. Isoflavone referred to in the present invention is the total amount of daidzin, genistin, daidzein, genistein, the quantitative determination of each component by reflux extraction of a certain amount of the sample and quantifying each component by high performance liquid chromatography, the total amount is calculated. To do. The peptide may be any plant or animal material as long as it contains a peptide that is a hydrolyzate of protein. It is preferable that the peptides have a high content, and a hydrolyzate of soybean protein is particularly preferable. In the quantification of the peptide referred to in the present invention, nitrogen is quantified by a known Kjeldahl method or a method similar thereto in a fixed amount of a test substance, and a nitrogen coefficient (6.25)
Calculate by multiplying by. Healthy foods are foods (all foods and drinks; however, pharmaceuticals and quasi-drugs are not included), supplemented with nutritional components, or sold as those suitable for special health uses. Says the food used for food.
【0010】大豆由来とは、大豆やその一部分又はそれ
らの分級・分離・粉砕・混合・加熱・抽出・加水分解・
冷蔵・冷凍・乾燥・成形等から選択、組み合わせによる
加工処理を指す。大豆由来のイソフラボンは例示とし
て、特公平04−48417号公報(調湿・加熱・剥皮
・大豆胚軸採取)、特公平05−33016号公報(ア
ルカリ液浸漬・焙煎・大豆胚軸処理法)、特公平07−
238089号公報(多量のイソフラボン化合物を含有
するタンパク質単離物及びその製造方法)などがある。
大豆由来のペプチドは例示として、特開平07−264
993号公報(大豆ペプチド混合物の製造法)、特開平
07−284369号公報(ペプチド組成物及びその製
造方法)、特開平09−132592号公報(ペプチド
結合水解物の製造法及びこれに用いるペプチド結合水解
剤)、特表平09−507478号公報(ペプチド生成
物の製造法と得られた生成物)などがある。乾燥重量と
は、一般の乾燥減量法(105℃で4時間)による乾燥
固形分の重量%である。Soybean-derived means soybean or a part thereof or classification, separation, pulverization, mixing, heating, extraction, hydrolysis, etc. thereof.
It refers to processing by selecting from refrigeration, freezing, drying, molding, etc., and combining. Soybean-derived isoflavones are, for example, Japanese Patent Publication No. 04-48417 (humidity control, heating, peeling, soybean hypocotyl collection), Japanese Patent Publication No. 05-33016 (alkaline solution immersion, roasting, soybean hypocotyl treatment method). , Special fair 07-
238089 (protein isolate containing a large amount of isoflavone compound and method for producing the same) and the like.
The peptide derived from soybean is, for example, JP-A-07-264.
No. 993 (Production method of soybean peptide mixture), JP-A-07-284369 (Peptide composition and production method thereof), JP-A-09-132592 (Peptide-bonded hydrolyzate production method and peptide bond used therefor) Water-decomposing agent), Japanese Patent Publication No. 09-507478 (manufacturing method of peptide product and obtained product). The dry weight is the weight% of the dry solid content by a general dry weight loss method (105 ° C. for 4 hours).
【0011】イソフラボンは、エストロゲン(女性ホル
モン)と同じ作用を示す植物エストロゲンの一種であ
り、骨からのカルシウム(Ca。以下、略記する)の溶
出を防ぐと言われている。大豆にはイソフラボン成分を
多く含んでおり、ダイジン、ゲニスチン、グリスチン等
の配糖体とそれらから糖がとれたアグリコンや、配糖体
にマロニル基が結合したマロニル体等の形態で存在して
いる。したがって、大豆ペプチドにもイソフラボンが微
量含まれているため、これをたん白質源として利用する
ことで更に効果が高くなることが期待される。イソフラ
ボンは日本人で一日平均10〜20mg摂取されている
が、骨粗鬆症などの予防には更に一日10〜20mgを
追加して摂取するのが望ましいと言われている。[0012] Isoflavone is a kind of phytoestrogen which exhibits the same action as estrogen (female hormone), and is said to prevent elution of calcium (Ca, hereinafter abbreviated) from bone. Soybean contains a large amount of isoflavone components, and exists in the form of glycosides such as daidzin, genistin, and gristin, aglycones from which sugars have been removed, and malonyl bodies in which a malonyl group is bound to glycosides. . Therefore, since soybean peptide also contains a very small amount of isoflavone, it is expected that the effect will be further enhanced by using this as a protein source. Japanese people take isoflavone 10 to 20 mg on average on an average daily basis, but it is said that it is desirable to additionally take 10 to 20 mg daily for the prevention of osteoporosis and the like.
【0012】たん白質は、Caと同様に骨の主成分であ
り、骨量を増やすためにはCa強化だけでは不十分であ
るため、同時にたん白質の摂取も不可欠である。一方、
たん白質は、筋肉の構成成分であり、加齢とともに衰え
る筋肉量を増やすためには、たん白質の摂取が不可欠で
ある。[0012] Protein, like Ca, is the main component of bone, and since Ca strengthening alone is not sufficient to increase bone mass, it is also essential to take in protein at the same time. on the other hand,
Protein is a component of muscles, and in order to increase the amount of muscle that declines with age, protein intake is essential.
【0013】ペプチドは、たん白質が加工されたものと
も言え、たん白質の効能と類似する点があるが、特に次
の点において優れている。すなわち、通常のたん白質よ
りも体内への吸収速度が速く、効率的に骨たん白質、筋
たん白質の合成に利用される。また、消化・吸収による
胃腸への負担が少ないため、高齢者や病人において不足
しがちなたん白質源として非常に有効である。たん白質
の一日所要量は平均60gであるが、高齢者はたん白質
の摂取が下がり気味で、かつ消化・吸収力が低い。従っ
てたん白質源として消化のよいペプチドを、更に一日に
5g程度摂取すれば筋肉の補強に有効であると期待出来
る。[0013] Peptides can be said to be processed proteins and have similarities to the effects of proteins, but are particularly excellent in the following points. That is, the absorption rate into the body is higher than that of normal proteins, and it is efficiently used for the synthesis of bone proteins and muscle proteins. Moreover, since the burden on the gastrointestinal tract due to digestion and absorption is small, it is very effective as a protein source that tends to be insufficient in elderly people and sick people. The daily requirement for protein is 60 g on average, but elderly people tend to have low intake of protein and have low digestion and absorption. Therefore, it can be expected that ingesting 5 g of a digestible peptide as a protein source further daily will be effective for muscle strengthening.
【0014】本発明は、イソフラボンとペプチドを併含
し、乾燥重量でイソフラボン含量がペプチドに対し、
0.2%以上である食品及び飲料である。上記の様にイ
ソフラボンとペプチドを適当な比率で摂取することによ
り、効果的に不足分を補給して高齢者の骨折などを防止
するのに役立つと考えられる。イソフラボン含量はペプ
チドに対し、共に乾燥重量で0.2%以上であるのが良
く、好ましくは0.4%以上である。0.4%以上の上
限については、イソフラボンとペプチドを組み合せる食
品及び飲料の種類・摂取量と、人1日栄養所要量の中の
摂取割合、などからイソフラボン含量のペプチドに対す
る%値を設定すれば良い。The present invention contains both isoflavones and peptides, and the isoflavone content of the dry weight of the peptides is
Foods and beverages with 0.2% or more. It is considered that ingestion of isoflavone and peptide at an appropriate ratio as described above will effectively supplement the shortage and help prevent bone fracture and the like in the elderly. The isoflavone content is preferably 0.2% or more and more preferably 0.4% or more by dry weight with respect to the peptide. Regarding the upper limit of 0.4% or more, it is necessary to set the% value of the isoflavone content to the peptide based on the types and intakes of foods and beverages that combine isoflavones and peptides, and the intake ratio in human daily nutritional requirements. Good.
【0015】本発明のイソフラボン含有物は、例えば大
豆胚軸等から、更にはその乾熱加熱品、或いはその水溶
性溶媒抽出物、有機溶媒抽出物を用いることが出来、こ
れらの製造法は公知の方法によっても良い。本発明のペ
プチド含有物は、例えば分離大豆たん白をプロテアーゼ
により加水分解した大豆ペプチドを用いることが出来、
これらの製造法は公知の方法によっても良い。The isoflavone-containing product of the present invention can be used, for example, from soybean hypocotyl and the like, and can also be used as a dry-heated product thereof, or a water-soluble solvent extract or organic solvent extract thereof, and their production methods are known. It is also possible to use The peptide-containing material of the present invention can use, for example, soybean peptide obtained by hydrolyzing isolated soybean protein with a protease,
The manufacturing method thereof may be a known method.
【0016】[0016]
【実施例】以下に本発明の有効性を実施例と共に示す
が、これらの例示によって本発明の技術思想が限定され
るものではない。EXAMPLES The effectiveness of the present invention will be shown below together with examples, but the technical idea of the present invention is not limited by these examples.
【0017】[0017]
【実施例1】骨強化と筋肉強化の効果
卵巣切除モデルラットを用い、ペプチドとイソフラボン
を併含する飲食物の投与によって骨粗鬆症を原因とする
骨量減少の抑制効果及び筋肉強化の効果を検討した。
(飼育方法)飼育は卵巣切除モデルラット(15週齢、
妊娠可能な週齢)をコントロール群(C群)とイソフラ
ボン・ペプチド摂取群(IP群)とに分け、6匹を1群
として、4週間飼育した。C群は固形飼料「MF」(水
分7.9%、たん白質23.3%、炭水化物57.7
%、オリエンタル酵母工業株式会社製。以下、同様)2
0gを毎日、経口投与した。IP群は同じ固形飼料に対
し、ペプチド含有物・大豆由来の「ハイニュート−DC
5」(水分6%、ペプチド75%、イソフラボン0.0
7%、不二製油株式会社製。以下、同様)をたん白質と
して3.5重量%、及び大豆胚軸抽出物・イソフラボン
含有物「ソヤフラボン−E」(水分5%、イソフラボン
含量4%。不二製油株式会社製。以下、同様)をイソフ
ラボンとして10mg重量%それぞれ添加したものを同
様に毎日、経口投与した。飼料以外の条件はC群、IP
群とも同じにして行った。
(トレーニング方法と筋肉量測定)ラットに1日当たり
に決められた運動負荷を与えるため、浴槽内で遊泳運動
をさせた。ラットが泳ぐことが出来なくなった時点を最
大遊泳時間とした。28日間の飼育終了後、ラットの腓
腹筋(ひふくきん)、四頭筋(しとうきん)の重量をそ
れぞれ測定した。
(骨密度測定)大腿骨を摘出し、その湿重量とピクノメ
ーターにより体積を測定し、骨密度を算出した。筋重量
測定及び骨密度の測定結果を表1に示した。Example 1 Effects of Bone Reinforcement and Muscle Reinforcement Using an ovariectomized model rat, the effect of suppressing bone loss caused by osteoporosis and the effect of muscle strengthening were examined by administration of food and drink containing peptide and isoflavone. . (Breeding method) Ovariectomized model rats (15 weeks old,
Pregnant weeks) were divided into a control group (C group) and an isoflavone peptide ingestion group (IP group), and 6 animals were grouped and raised for 4 weeks. Group C is a solid feed "MF" (water 7.9%, protein 23.3%, carbohydrates 57.7).
%, Manufactured by Oriental Yeast Co., Ltd. The same applies hereinafter) 2
0 g was orally administered daily. For the IP group, the same solid feed was treated with the peptide-containing and soybean-derived "High New-DC
5 "(water 6%, peptide 75%, isoflavone 0.0
7%, manufactured by Fuji Oil Co., Ltd. The same shall apply hereinafter) as a protein, and 3.5% by weight of soybean hypocotyl extract / isoflavone-containing material "Soyaflavone-E" (water content 5%, isoflavone content 4%; manufactured by Fuji Oil Co., Ltd .; hereinafter the same). Was added as isoflavone in an amount of 10 mg% by weight, and was orally administered daily in the same manner. Conditions other than feed are group C, IP
The group was the same. (Training Method and Measurement of Muscle Mass) In order to give a given exercise load per day to the rats, the rats were allowed to take a swimming exercise in a bathtub. The maximum swimming time was the time when the rat was unable to swim. After the breeding for 28 days, the weight of the gastrocnemius muscle (Hifukukin) and quadriceps muscle (Shitokin) of the rat was measured. (Measurement of Bone Density) The femur was extracted, its wet weight and the volume were measured with a pycnometer, and the bone density was calculated. The results of muscle weight measurement and bone density measurement are shown in Table 1.
【0018】 表1 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− C群 IP群 有意差(p) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 腓腹筋 0.96±0.02 1.07±0.02 <0.05 四頭筋 1.37±0.03 1.46±0.02 <0.05 骨密度( g/ ml) 1.31±0.03 1.48±0.04 <0.05 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− p<0.05は、危険率5%で有意差あり。[0018] Table 1 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Group C IP group Significant difference (p) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Gastrocnemius 0.96 ± 0.02 1.07 ± 0.02 <0.05 Quadriceps 1.37 ± 0.03 1.46 ± 0.02 <0.05 Bone density (g / ml) 1.31 ± 0.03 1.48 ± 0.04 <0.05 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− When p <0.05, there was a significant difference at a risk rate of 5%.
【0019】表1の結果より、イソフラボン及びペプチ
ド摂取群は対照群と比較して骨密度及び筋肉量が有意に
高くなり、骨強化及び筋肉強化の効果が認められた。From the results shown in Table 1, the isoflavone and peptide ingested groups had significantly higher bone density and muscle mass than the control group, and the effects of bone strengthening and muscle strengthening were recognized.
【0020】[0020]
【実施例2】飲料調製と筋肉増強効果
表2に示す配合で原料を調合し、UHT殺菌を行い、本
発明に言う飲料を調製した。[Example 2] Beverage preparation and muscle-enhancing effect A raw material was prepared according to the composition shown in Table 2 and subjected to UHT sterilization to prepare a beverage according to the present invention.
【0021】 表2 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− ペプチド及びイソフラボン併含の飲料配合(200g) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− ペプチド含有物 6.7 g 大豆胚軸抽出物 0.25g 砂糖 16.0 g グレープフルーツ濃縮果汁 1.2 g 酸味料 1.0 g 乳酸カルシウム 0.8 g ビタミンD3 17I U グレープフルーツ香料 0.1 g 水 172.15g −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 合計 200.0 g −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−[0021] Table 2 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Beverage containing both peptide and isoflavone (200g) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Peptide inclusion 6.7 g Soybean hypocotyl extract 0.25g 16.0 g sugar Grapefruit juice concentrate 1.2 g Acidulant 1.0 g Calcium lactate 0.8 g Vitamin D3 17I U Grapefruit flavor 0.1 g Water 172.15g −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 200.0 g in total −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
【0022】本飲料はペプチドに対してイソフラボン
0.3%を含有する。本飲料を200g摂取することに
より、大豆ペプチドをたん白質として5.0g、イソフ
ラボンを15mg補給することができ、更にカルシウム
100mg(日本人1日栄養所要量の1/6)とビタミ
ンD17IU(日本人1日栄養所要量の1/6)も補給
することができる様に設定した。試験は大学運動部員1
0名をパネラーとして、本飲料投与群(P群)と本飲料
からペプチド及びイソフラボンを省略した対照群(C
群) の2群に分けて行った(各群5名)。各群のパネラ
ーは何れも週3日のウェイトトレーニングを組み入れる
ことにし、P群、C群とも各飲料を1日2回(練習後お
よび就寝前に各1回)、3ヶ月間摂取させた。筋力の測
定は「パワーマックスV」(コンビ社製)を用いてパネ
ラーの体重の0.05倍の負荷強度で10秒間を2回全
力で漕がせ、その時の最大回転数を求めることで行っ
た。表3に、3ケ月までの各群の最大回転数の測定結果
を示した。The beverage contains 0.3% isoflavones based on the peptide. By ingesting 200g of this beverage, soybean peptide as a protein can be supplemented with 5.0g and isoflavone with 15mg, and further 100mg of calcium (1/6 of Japanese daily nutritional requirement) and vitamin D17IU (Japanese). It was set to be able to supplement 1/6 of the daily nutritional requirement. The exam is a member of university sports club
There were 0 panelists, and this beverage administration group (P group) and a control group (C which omitted peptide and isoflavone from this beverage)
It was divided into two groups (5 groups each). Each group of panelists decided to incorporate weight training 3 days a week, and both groups P and C were allowed to drink each beverage twice a day (once after practice and before bedtime) for 3 months. The muscular strength was measured by using "Power Max V" (manufactured by Combi Co., Ltd.) to perform full-power rowing for 10 seconds twice with a load intensity of 0.05 times the weight of the panelist, and finding the maximum number of revolutions at that time. It was Table 3 shows the measurement results of the maximum rotation speed of each group up to 3 months.
【0023】 表3 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 各群の最大回転数の開始時からの変化量の比較 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− P群 C群 有意差(p) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 開始時 0.0 0.0 − 1ヶ月後 11.0 3.0 <0.1 3ヶ月後 19.0 9.0 <0.1 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− p<0.1は、危険率10%で有意差あり。[0023] Table 3 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Comparison of the amount of change from the start of the maximum rotation speed of each group −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− P group C group Significant difference (p) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Start 0.0 0.0 − 1 month later 11.0 3.0 <0.1 3 months later 19.0 9.0 <0.1 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− When p <0.1, there is a significant difference at a risk rate of 10%.
【0024】表3に示す通り、各群の最大回転数を比較
すると、C群に比べ、P群は有意に増加した。これによ
り、大豆ペプチドと大豆イソフラボンを併含する飲食物
には人においても筋肉増強効果が認められた。As shown in Table 3, when the maximum rotational speeds of the respective groups were compared, the P group significantly increased as compared with the C group. From this, it was confirmed that the food and drink containing soybean peptide and soybean isoflavone also had a muscle enhancing effect in humans.
【0025】[0025]
【実施例3】ペプチド及びイソフラボン併含ゼリー
表4に示す配合で原料を調合し、20g/個のゼリーを
調製した。
表4
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド及びイソフラボン併含のゼリー配合(100g)
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド含有物 6.7g
大豆胚軸抽出物 0.25g
砂糖 15.0g
グレープフルーツ濃縮果汁 10.0g
ゲル化剤 1.2g
リキュール 1.5g
グレープフルーツ香料 0.2g
水 65.15g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
合計 100.0g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
本ゼリーを5個摂取することにより、表2に示した飲料
と同量のペプチド及びイソフラボンを摂取することが出
来る。本ゼリーを、栄養補給を目的とした健康食品に応
用することが出来る。Example 3 Jelly Containing Peptide and Isoflavone A raw material was prepared in the composition shown in Table 4 to prepare 20 g / piece of jelly. Table 4 Jelly formulation (100 g) containing both peptide and isoflavone --------------------------------------. ------------------ Peptide-containing product 6.7 g Soybean hypocotyl extract 0.25 g Sugar 15. 0 g Grapefruit concentrated fruit juice 10.0 g Gelling agent 1.2 g Liqueur 1.5 g Grapefruit flavor 0.2 g Water 65.15 g ------------------------------------ ------------- Total 100.0 g ---------------------------------------- By ingesting 5 of this jelly, the same amount of peptide and isoflavone as in the beverages shown in Table 2 can be ingested. This jelly can be applied to a health food for the purpose of nutritional supplementation.
【0026】[0026]
【実施例4】ペプチド及びイソフラボン併含クッキー
表5に示す配合で原料を調合し、10g/枚のクッキー
を調製した。
表5
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド及びイソフラボン併含のクッキー配合(100g)
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド含有物 4.7g
大豆胚軸粉砕物 1.0g
薄力粉 45.6g
マーガリン 27.5g
上白糖 17.6g
液卵 3.0g
食塩 0.6g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
合計 100.0g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
用いた大豆胚軸粉砕物は、イソフラボン含有物「ソヤフ
ラボン−RS」(水分5%、イソフラボン含量1%。不
二製油株式会社製。以下、同様)であった。本クッキー
を10枚摂取することにより、ペプチドを3.5g及び
イソフラボンを13mg摂取することが出来る。本クッ
キーは、栄養補給を目的とした健康食品に応用すること
が出来る。Example 4 Cookie Containing Peptide and Isoflavone The raw materials were prepared according to the composition shown in Table 5, and 10 g / piece of cookie was prepared. Table 5 Cookie composition containing both peptide and isoflavone (100 g) --- ------------------------------------------- Peptide-containing product 4.7 g Soybean hypocotyl ground product 1.0 g Soft flour 45. 6 g Margarine 27.5 g White sugar 17.6 g Liquid egg 3.0 g Salt 0.6 g ------------------------------------- ----- Total 100.0 g ---------------------------------- Soybean hypocotyl crushed product used Was an isoflavone-containing material "Soyaflavone-RS" (water content 5%, isoflavone content 1%. Fuji Oil Co., Ltd .; hereinafter the same). By ingesting 10 of these cookies, 3.5 g of peptide and 13 mg of isoflavone can be ingested. This cookie can be applied to a health food for the purpose of nutritional supplementation.
【0027】[0027]
【実施例5】ペプチド及びイソフラボン併含タブレット
表6に示す配合で原料を調合し、1g/粒のタブレット
を調製した。
表6
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド及びイソフラボン併含のタブレット配合(100g)
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド含有物 67.0g
大豆胚軸抽出物 2.5g
乳糖 25.5g
炭酸カルシウム 2.0g
乳化剤 3.0g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
合計 100.0g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
本タブレットを10粒摂取することにより、ペプチドを
5.0g及びイソフラボンを15mg補給することが出
来、更にカルシウムを80mg補給することが出来る。
本タブレットは、栄養補給を目的とした健康食品に応用
することが出来る。Example 5 Tablet Containing Peptide and Isoflavone Raw materials were prepared according to the formulation shown in Table 6 to prepare tablets of 1 g / grain. Table 6 ------------------------------------------- Tablet formulation containing both peptide and isoflavone (100 g)- ------------------------------------------- Peptide-containing product 67.0 g Soybean hypocotyl extract 2.5 g Lactose 25. 5 g Calcium carbonate 2.0 g Emulsifier 3.0 g --------------------------------------------------------------------------------------- Total 100.0 g- ------------------------------------------------- By ingesting 10 tablets of the present tablet, 5.0 g of the peptide and It is possible to replenish 15 mg of isoflavones and 80 mg of calcium.
This tablet can be applied to health food for the purpose of nutritional supplementation.
【0028】[0028]
【実施例6】ペプチド及びイソフラボン併含紅茶飲料
表7に示す配合で原料を調合し、100g/杯の紅茶飲
料を調製した。
表7
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド及びイソフラボン併含の紅茶飲料配合(100g)
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
ペプチド含有物 6.7g
大豆胚軸抽出物 0.25g
顆粒紅茶 0.60g
全脂粉乳 2.0g
砂糖 7.5g
紅茶香料 0.03g
水 82.92g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
合計 100.0g
−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
本紅茶飲料を100g摂取することにより、ペプチドを
5.0g及びイソフラボンを15mg補給することが出
来る。本紅茶飲料は、栄養補給を目的とした健康食品に
応用することが出来る。Example 6 Black Tea Beverage Containing Peptide and Isoflavone The raw materials were blended according to the formulation shown in Table 7 to prepare 100 g / cup of black tea beverage. Table 7 -------------------------------------------- A tea beverage formulation containing both peptide and isoflavone (100 g)- ----------------------------------------- Peptide-containing product 6.7 g Soybean hypocotyl extract 0.25 g Granulated black tea 0.60 g Whole milk powder 2.0 g Sugar 7.5 g Black tea flavor 0.03 g Water 82.92 g ------------------------------. ---------- Total 100.0 g ----------------------------- By ingesting 100 g, 5.0 g of peptide and 15 mg of isoflavone can be supplemented. This tea beverage can be applied to a health food for the purpose of nutritional supplementation.
【0029】実施例1及び2の結果より、イソフラボン
及びペプチドを併含する食品或いは飲料には、骨粗鬆症
を原因とする骨量の減少を抑制することにより、骨自身
を強化する効果、更に骨の周りを広く包んでいる筋肉を
強化することによって、骨への過重による負担を和ら
げ、転倒などによる骨折を防止する二重の効果が期待で
きるものであることが示唆された。From the results of Examples 1 and 2, the food or beverage containing both isoflavone and peptide has the effect of strengthening the bone itself by suppressing the decrease of the bone mass caused by osteoporosis, and further It was suggested that by strengthening the muscles that wrap around the body, it is possible to reduce the burden of excessive load on the bone and to expect a double effect of preventing fractures such as falls.
【0030】[0030]
【発明の効果】本発明により、イソフラボンとペプチド
を併含する食品及び飲料が得られた。これにより、例え
ばイソフラボン含有量4重量%の大豆胚軸抽出物0.2
5部と、ペプチド含有量75重量%の大豆ペプチド6.
7部を飲料200部中に配合し、イソフラボン15m
g、ペプチド5gを含有する飲料により、骨の強化とそ
の骨を広く取り巻く筋肉の強化に役立ち得る製品が提供
できて、高齢化社会にも貢献できる。INDUSTRIAL APPLICABILITY According to the present invention, foods and beverages containing both isoflavone and peptides were obtained. As a result, for example, soybean hypocotyl extract 0.2 with an isoflavone content of 4% by weight is obtained.
5 parts and soy peptide with a peptide content of 75% by weight 6.
7 parts were mixed with 200 parts of the beverage, and isoflavone 15 m
The beverage containing g and 5 g of peptide can provide a product that can be useful for strengthening bones and strengthening muscles that widely surround the bones, and can also contribute to an aging society.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 FI A23L 2/66 A23L 2/00 F (58)調査した分野(Int.Cl.7,DB名) A23L 1/29 - 1/305 A23L 1/20 A23J 3/16 - 3/34 ─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. 7 Identification code FI A23L 2/66 A23L 2/00 F (58) Fields investigated (Int.Cl. 7 , DB name) A23L 1/29-1 / 305 A23L 1/20 A23J 3/16-3/34
Claims (5)
ラボン含量がペプチドに対し0.4%以上、ペプチド含
有物が乾燥重量で2重量%以上である食品及び飲料。1. An isoflavone is added, and the isoflavone content is 0.4% or more based on the dry weight of the peptide, and the isoflavone- containing peptide is included.
Foods and beverages with a content of 2% by weight or more in terms of dry weight .
品、或いはその水溶性溶媒抽出物または有機溶媒抽出物
から得られたものである請求項1の食品及び飲料。2. The food and beverage according to claim 1, wherein the isoflavone is obtained from soybean hypocotyl, its dry-heated product, or its water-soluble solvent extract or organic solvent extract.
により加水分解した大豆ペプチドである請求項1または
2の食品及び飲料。3. The food and beverage according to claim 1 or 2, wherein the peptide is soybean peptide obtained by hydrolyzing isolated soybean protein with a protease.
ボン含量が1重量%以上、用いるペプチド含有物が乾燥
重量でたん白質含量として50重量%以上のものである
請求項1から請求項3のいずれかに記載の食品。4. The isoflavone used has a dry weight of isoflavone content of 1% by weight or more, and the peptide-containing substance used has a dry weight of protein content of 50% by weight or more. Listed food.
類、ビタミン類、イソフラボン以外のCa吸収促進又は
Ca溶出抑制効果を持つ機能性素材等を含む請求項1か
ら請求項4のいずれかに記載の食品及び飲料。5. The method according to any one of claims 1 to 4, which contains a Ca (calcium) -containing material, minerals, vitamins, and functional materials other than isoflavone that have a Ca absorption promoting effect or a Ca elution suppressing effect. Food and drink.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP31354498A JP3430940B2 (en) | 1998-11-04 | 1998-11-04 | Foods and beverages combining isoflavones and peptides |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP31354498A JP3430940B2 (en) | 1998-11-04 | 1998-11-04 | Foods and beverages combining isoflavones and peptides |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2000139411A JP2000139411A (en) | 2000-05-23 |
JP3430940B2 true JP3430940B2 (en) | 2003-07-28 |
Family
ID=18042608
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP31354498A Ceased JP3430940B2 (en) | 1998-11-04 | 1998-11-04 | Foods and beverages combining isoflavones and peptides |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP3430940B2 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20030048427A (en) | 2000-10-13 | 2003-06-19 | 가부시키가이샤 야쿠루트 혼샤 | Bone metabolism improving agent |
JP5060691B2 (en) * | 2001-07-09 | 2012-10-31 | 雪印メグミルク株式会社 | Nutritional composition |
US20070231411A2 (en) * | 2003-09-19 | 2007-10-04 | Sunstar Inc. | Method of inhibiting alveolar bone resorption and periodontal membrane loss and composition for internal use to be used therein |
JP2006282573A (en) * | 2005-03-31 | 2006-10-19 | Morinaga & Co Ltd | Muscle damage-inhibitory composition and muscle damage relief-promoting composition |
JP4940047B2 (en) * | 2007-08-03 | 2012-05-30 | 花王株式会社 | Container drink |
JP2010057428A (en) * | 2008-09-04 | 2010-03-18 | Shiseido Co Ltd | Food and drink |
-
1998
- 1998-11-04 JP JP31354498A patent/JP3430940B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
JP2000139411A (en) | 2000-05-23 |
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