JP3379202B2 - Enzyme electrode - Google Patents
Enzyme electrodeInfo
- Publication number
- JP3379202B2 JP3379202B2 JP06414794A JP6414794A JP3379202B2 JP 3379202 B2 JP3379202 B2 JP 3379202B2 JP 06414794 A JP06414794 A JP 06414794A JP 6414794 A JP6414794 A JP 6414794A JP 3379202 B2 JP3379202 B2 JP 3379202B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- enzyme
- electron transfer
- substance
- transfer substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Description
【０００１】[0001]
【産業上の利用分野】本発明は、体液または食品などの
試料液における、被検出成分の濃度を簡易に定量する酵
素電極に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an enzyme electrode for easily quantifying the concentration of a component to be detected in a body fluid or a sample liquid such as food.
【０００２】[0002]
【従来の技術】酵素電極は、少なくとも対極と酵素を固
定化した測定極からなり、試料液中の被検出物質を測定
極で特異的に感知し、酸化還元酵素の反応にともなう物
質変化量を、電子伝達物質を介して電気化学的な変化量
に変換して検知するものである。2. Description of the Related Art An enzyme electrode is composed of at least a counter electrode and a measuring electrode on which an enzyme is immobilized. The enzyme electrode specifically senses a substance to be detected in the sample solution and detects the amount of change in the substance due to the reaction of oxidoreductase. , Is converted into an electrochemical change amount through an electron transfer substance and detected.
【０００３】従来、測定極は、白金、銀、などの金属ま
たはカーボンなどの導電性粉末から形成された電極層の
上に、固定化酵素層または／および電子伝達物質層を被
覆した積層の形態を有し、これまで、被検出物質を迅速
かつ高感度に検知するための技術改良がなされてきた。Conventionally, the measuring electrode is in the form of a laminate in which an immobilized enzyme layer or / and an electron mediator layer is coated on an electrode layer formed of a conductive powder of metal such as platinum or silver or carbon or carbon. Therefore, technical improvements have been made to detect a substance to be detected quickly and with high sensitivity.
【０００４】しかしながら積層法では、層間の接触面は
平面であるために、製造工程中または測定時に剥離や脱
落する問題があった。さらに、この様な接着性が不十分
な電極では、層間の電子授受がスムーズにおこなわれ
ず、応答性の点で限界があった。However, in the laminating method, since the contact surface between the layers is a flat surface, there is a problem of peeling or dropping during the manufacturing process or during measurement. Further, in such an electrode having insufficient adhesiveness, electron transfer between layers is not smoothly performed, and there is a limit in responsiveness.
【０００５】この問題点を解決した方法として、酵素お
よび電子伝達物質を、導電性粉末とバインダー溶液と共
にインキとして混合し、絶縁性基板上に固定化する混合
法が提案されている。混合法より形成した測定極では、
電極に相当する導電性粉末中に、酵素および電子伝達物
質が点在している形態をもつ。そのため、電極中の各成
分間の接触面積は増加し、効率的な電子授受が可能とな
る。その結果、応答性は増大する。As a method for solving this problem, there has been proposed a mixing method in which an enzyme and an electron transfer substance are mixed as an ink with a conductive powder and a binder solution and fixed on an insulating substrate. In the measuring electrode formed by the mixing method,
It has a form in which an enzyme and an electron transfer substance are scattered in a conductive powder corresponding to an electrode. Therefore, the contact area between each component in the electrode is increased, and efficient electron transfer is possible. As a result, the responsiveness is increased.
【０００６】さらに、混合法が有用な点は、酵素と電子
伝達物質を応答性に優れた配合比に設定できることにあ
る。積層法の場合、応答性は層間における接触面積に依
存する。そのため、積層厚などで絶対量を増加させて
も、感度の向上は困難である。これに対し、混合法で
は、電極中の各成分の粒子または分子レベルでの接触が
主体であるため、最適な配合比を設定することが可能で
あり、さらに感度向上を導くことができる。Further, the usefulness of the mixing method is that the mixing ratio of the enzyme and the electron transfer substance can be set to have excellent responsiveness. In the case of the lamination method, the responsiveness depends on the contact area between the layers. Therefore, it is difficult to improve the sensitivity even if the absolute amount is increased by the layer thickness or the like. On the other hand, in the mixing method, since the contact of each component in the electrode at the particle or molecular level is the main component, it is possible to set an optimum blending ratio and further improve the sensitivity.
【０００７】[0007]
【発明が解決しようとする課題】よって本発明が解決し
ようとする課題は、測定極の構成成分である酵素と電子
伝達物質の総量および配合比を設定することにより、応
答性を増し、高感度な酵素電極を提供する事である。Therefore, the problem to be solved by the present invention is to increase the responsiveness and high sensitivity by setting the total amount and the compounding ratio of the enzyme and the electron transfer substance which are the constituent components of the measuring electrode. To provide a simple enzyme electrode.
【０００８】[0008]
【課題を解決するための手段】本発明は上記の課題を解
決するために、少なくとも導電性粉末、バインダー、酵
素と電子伝達物質を含む導電性インキを、絶縁性基板上
に塗工後、加熱乾燥することにより形成した測定極にお
いて、酵素と電子伝達物質の総量が電極の１０〜４０重
量％からなる酵素電極である。また、前記酵素電極中の
酵素と電子伝達物質の重量配合比が、１／９〜７／３で
ある上記の酵素電極である。In order to solve the above-mentioned problems, the present invention applies a conductive ink containing at least a conductive powder, a binder, an enzyme and an electron transfer substance onto an insulating substrate and then heats it. In the measurement electrode formed by drying, the total amount of the enzyme and the electron transfer substance is 10 to 40 % by weight of the electrode, which is an enzyme electrode. Further, in the above enzyme electrode, the mixing ratio by weight of the enzyme and the electron transfer substance in the enzyme electrode is 1/9 to 7/3.
【０００９】以下より詳細に説明する。測定極中の酵素
および電子伝達物質は、試料液中の被検出物質を感知
し、電極である導電性粉末へ電子を伝達する。そのため
電極中に占める重量比が不足した場合、応答性は低下す
る。逆に過剰となった場合でも、絶対量が減少した導電
性粉末では電子量に対応できず、応答性は低下する。従
って、高感度な応答は、前記の様に酵素と電子伝達物質
の総量を５〜５０重量％、より好ましくは１０〜４０重
量％とすることで達成される。The details will be described below. The enzyme and the electron transfer substance in the measurement electrode sense the substance to be detected in the sample solution and transfer the electron to the conductive powder which is the electrode. Therefore, when the weight ratio occupied in the electrode is insufficient, the responsiveness decreases. On the contrary, even when the amount is excessive, the conductive powder having a reduced absolute amount cannot cope with the amount of electrons and the responsiveness deteriorates. Therefore, a highly sensitive response is achieved by setting the total amount of the enzyme and the electron transfer substance to 5 to 50% by weight, more preferably 10 to 40% by weight, as described above.
【００１０】また、電極中の酵素と電子伝達物質の重量
配合比を、１／９〜７／３に設定することの意味は、両
成分の間にも、より効率的な電子授受がなされる配合重
量比があり、前記したように１／９〜７／３、より好ま
しくは２／８〜６／４とすることで高感度な応答が達成
される。Further, the weight ratio of the enzyme and the electron transfer substance in the electrode is set to 1/9 to 7/3, which means that more efficient electron transfer can be performed between both components. There is a blending weight ratio, and as described above, a highly sensitive response can be achieved by setting it to 1/9 to 7/3, and more preferably 2/8 to 6/4.
【００１１】被検出物質を感知する測定極は、対極と併
用することにより酵素電極として作用し、両極間にある
一定の電圧を印加した場合、流れる電流値から成分濃度
を検知することができる。被検出物質を含む検液に酵素
電極を接触または浸漬させ、電圧を印加することで測定
は開始できる。測定極中の酵素は被検出物質を特異的に
感知し、酵素反応にともなう電子移動は、電子伝達物質
を介して導電性粉末が受けて電流値として検出される。
測定極の構成成分は、少なくとも酵素、電子伝達物質、
電極の基盤となる導電性粉末、これらを密着させ絶縁性
基板に固定するためのバインダーよりなる。The measuring electrode which senses the substance to be detected acts as an enzyme electrode when used in combination with the counter electrode, and when a constant voltage between both electrodes is applied, the concentration of the component can be detected from the flowing current value. The measurement can be started by contacting or immersing the enzyme electrode in a test solution containing the substance to be detected and applying a voltage. The enzyme in the measuring electrode specifically senses the substance to be detected, and the electron transfer accompanying the enzymatic reaction is detected by the conductive powder through the electron transfer substance and detected as a current value.
The components of the measuring electrode are at least an enzyme, an electron mediator,
It is composed of a conductive powder serving as a base of the electrode and a binder for adhering these to each other and fixing them to the insulating substrate.
【００１２】バインダーは、測定極の構成成分を絶縁性
基板に固定化する役割を担う。通常、有機溶剤に溶解さ
せるか、または水溶液として用い、導電性インキの粘度
を調節する用途も兼ねる。具体的には、でんぷん系，セ
ルロース系，アルギン酸系，ガム類，タンパク質系など
の天然高分子類、アクリル系，ポリビニルアルコール
系，ポリビニルブチラール系，塩化ビニル系，酢酸ビニ
ル共重合体系，ポリアミド系，ポリエステル系，ポリウ
レタン系，ポリスチレン系，などの合成高分子樹脂類が
挙げられ、２種類以上の混合系を用いることもできる。
水溶性バインダーを使用する場合には、測定中に電極部
の溶解、脱落が起きないように非水溶性バインダーと混
合して用いる。The binder plays a role of fixing the constituent components of the measuring electrode to the insulating substrate. Usually, it is also dissolved in an organic solvent or used as an aqueous solution for the purpose of adjusting the viscosity of the conductive ink. Specifically, starch-based, cellulose-based, alginic acid-based, gums, protein-based and other natural polymers, acrylic-based, polyvinyl alcohol-based, polyvinyl butyral-based, vinyl chloride-based, vinyl acetate copolymer-based, polyamide-based, Synthetic polymer resins such as polyester-based, polyurethane-based, polystyrene-based and the like can be mentioned, and a mixed system of two or more kinds can also be used.
When a water-soluble binder is used, it is mixed with a non-water-soluble binder so that the electrode part does not dissolve or fall off during measurement.
【００１３】有機溶剤は、上記の導電性インキのバイン
ダーおよび他の構成成分を均一に溶解または分散させる
ものが好ましい。また、構成成分に対して安定であるこ
とも必要である。具体的には、アルコール系、エステル
系、芳香族炭化水素系、脂肪族炭化水素系などが用いら
れる。The organic solvent preferably dissolves or disperses the binder of the conductive ink and other constituents uniformly. It also needs to be stable to the constituents. Specifically, alcohol-based, ester-based, aromatic hydrocarbon-based, aliphatic hydrocarbon-based, etc. are used.
【００１４】電極に相当する導電性粉末としては、金属
およびその酸化物、カーボン、グラファイト等の顔料が
挙げられるが、コスト、表面の親水性およびインキ適性
等よりカーボンブラックが好ましい。Examples of the conductive powder corresponding to the electrodes include metals and oxides thereof, pigments such as carbon and graphite, and carbon black is preferable in terms of cost, hydrophilicity of the surface, suitability for ink and the like.
【００１５】電子伝達物質としては、酸化還元酵素の電
子伝達体として機能するレドックス化合物であれば特に
制限されない。具体的には、フェロセンおよびその誘導
体、ベンゾキノン、メチレンブルー、２，６−ジクロロ
インドフェノール、金属シアン化錯体等が挙げられる。The electron transfer substance is not particularly limited as long as it is a redox compound that functions as an electron transfer agent of oxidoreductase. Specific examples include ferrocene and its derivatives, benzoquinone, methylene blue, 2,6-dichloroindophenol, and metal cyanide complex.
【００１６】インキ中に含有させる酵素は、酸化還元酵
素であれば特に制限なく、たとえばグルコースオキシダ
ーゼ、コレステロールオキシダーゼ、ラクテートオキシ
ダーゼ、アルコールオキシダーゼ、キサンチンオキシダ
ーゼ、ピルベートオキシダーゼ、アルデヒドオキシダー
ゼ等が用いられる。The enzyme contained in the ink is not particularly limited as long as it is a redox enzyme, and for example, glucose oxidase, cholesterol oxidase, lactate oxidase, alcohol oxidase, xanthine oxidase, pyruvate oxidase, aldehyde oxidase and the like are used.
【００１７】絶縁性基板としては、セラミック、ガラ
ス、ガラスエポキシ、プラスチック等、試料液により侵
されないものが用いられる。特に、安価で扱い易いポリ
塩化ビニル、ポリエステル、ポリエチレン、ポリプロピ
レン等のプラスチックフィルムが好ましい。As the insulating substrate, those which are not corroded by the sample liquid, such as ceramic, glass, glass epoxy, and plastic, are used. In particular, a plastic film made of polyvinyl chloride, polyester, polyethylene, polypropylene or the like which is inexpensive and easy to handle is preferable.
【００１８】塗工方法としては均一に薄く塗工できる方
法なら何でもよいが、印刷方法が好ましい。印刷方法と
しては、スクリーン印刷、グラビア印刷、グラビアオフ
セット印刷、ノズルコーティング、ディスペンサー印
刷、インキジェット印刷等が応用できる。例えば、スク
リーン印刷方法にて形成する場合には、インキ粘度が１
０〜２０００ポイズ程度になるよう調整し、スクリーン
編み目１００〜４００メッシュのスクリーン版を用いて
印刷することで、平滑性の高い電極部を形成することが
できる。Any coating method may be used as long as it can be uniformly and thinly coated, but a printing method is preferable. As a printing method, screen printing, gravure printing, gravure offset printing, nozzle coating, dispenser printing, ink jet printing and the like can be applied. For example, when the screen printing method is used, the ink viscosity is 1
It is possible to form an electrode portion having high smoothness by adjusting so as to be about 0 to 2000 poise and printing using a screen plate having screen stitches of 100 to 400 mesh.
【００１９】[0019]
【作用】電極の構成成分をインキとして混合し、塗工し
て形成し、酵素と電子伝達物質の総量を電極の５〜５０
重量％とした酵素電極では、各成分間の接触面積は増
し、酵素反応にともなう電子授受を効率的に行うことが
できる。その上、前記電極中の酵素と電子伝達物質の重
量配合比が、１／９〜７／３に設定することで高感度な
酵素電極を構成することができる。[Function] The components of the electrode are mixed as an ink and coated to form the total amount of the enzyme and the electron transfer substance in the range of 5 to 50%.
In the case of the enzyme electrode in which the content is% by weight, the contact area between the components is increased, and the electron transfer accompanying the enzyme reaction can be efficiently performed. Furthermore, by setting the weight ratio of the enzyme and the electron transfer substance in the electrode to 1/9 to 7/3, a highly sensitive enzyme electrode can be constructed.
【００２０】[0020]
＜実施例１＞酵素電極の一例として、グルコースオキシ
ダーゼ（以下、ＧＯＤと略す）により試料液中のグルコ
ースを測定する場合の例を示す。（表１）は、アセチレ
ンブラック（旭電化工業（株）製）、ポリビニルブチラ
ール樹脂（ＫＳ−１；積水化学工業（株）製）／ブチル
セロソルブ溶液、ＧＯＤ（メルク社製：８Ｕ／ｍｇ）、
フェリシアン化カリウムからなる導電性インキの組成表
を示したものである。<Example 1> As an example of an enzyme electrode, an example of measuring glucose in a sample solution using glucose oxidase (hereinafter abbreviated as GOD) will be described. (Table 1) shows acetylene black (manufactured by Asahi Denka Co., Ltd.), polyvinyl butyral resin (KS-1; manufactured by Sekisui Chemical Co., Ltd.) / Butyl cellosolve solution, GOD (manufactured by Merck: 8 U / mg),
1 is a composition table of a conductive ink made of potassium ferricyanide.
【００２１】[0021]
【表１】 [Table 1]
【００２２】インキＡ〜Ｅは、ＧＯＤとフェリシアン化
カリウムの配合比を３／７と固定しながらインキ中の絶
対重量を調節したものでる。その場合、アセチレンブラ
ック／ポリビニルブチラール樹脂の配合比は１／１、イ
ンキ固形分比は２５重量％となるよう設定した。調液
は、自動乳鉢で１５分間混合した。図１に示すように、
カーボンからなる対極１を設けたポリエステル基板２
に、調液した導電性インキをスクリーン印刷法により塗
工し、測定極３を形成した。最後に電極を一部残して絶
縁性のポリマー層４を設けた。The inks A to E are those in which the absolute weight in the ink is adjusted while fixing the compounding ratio of GOD and potassium ferricyanide to 3/7. In that case, the compounding ratio of acetylene black / polyvinyl butyral resin was set to 1/1, and the ink solid content ratio was set to 25% by weight. The prepared solution was mixed in an automatic mortar for 15 minutes. As shown in Figure 1,
Polyester substrate 2 provided with a counter electrode 1 made of carbon
Then, the prepared conductive ink was applied by a screen printing method to form a measuring electrode 3. Finally, an insulating polymer layer 4 was provided by leaving a part of the electrode.
【００２３】対極との間に０．７Ｖの直流電流を印加し
て３０秒後に増加する電流を測定した。グルコース濃度
と出力電流の関係を調べたところ、グルコース濃度０〜
５００ｍｇ／ｄｌの範囲で良好な応答が得られた。結果
を図２に示す。特に、ＧＯＤとフェリシアン化カリウム
の総量が、測定電極中に占める重量比で１０〜４０付近
でより高感度な応答性が観察された。A direct current of 0.7 V was applied between the counter electrode and the increasing current after 30 seconds was measured. When the relationship between glucose concentration and output current was investigated, glucose concentration 0 to
Good response was obtained in the range of 500 mg / dl. The results are shown in Figure 2. In particular, when the total amount of GOD and potassium ferricyanide was about 10 to 40 in terms of the weight ratio in the measurement electrode, a highly sensitive responsiveness was observed.
【００２４】＜実施例２＞カーボンブラック（コロンビ
アカーボン社製）、アクリル樹脂（ＢＲ−７７：三菱レ
イヨン（株）製）／酢酸セロソルブ溶液、ベンゾキノ
ン、ＧＯＤ（メルク社製：８Ｕ／ｍｇ）からなる導電性
インキｂ〜ｆ（表２）を自動乳鉢で１５分間混合し、ス
クリーン印刷法でポリエステル基板２上に測定極３を形
成した。カーボンブラック／アクリル樹脂の配合比は２
／１であり、両成分の測定極中における絶対重量は９０
重量％である。さらに、測定極中の絶対重量が１０重量
％となるよう、ＧＯＤ／ベンゾキノンを１／９〜７／３
の配合比で振った。ポリエステル基板２には、カーボン
からなる対極１が設けてあり、電極の一部を残して絶縁
性のポリマー層４を設けた。<Example 2> Carbon black (manufactured by Columbia Carbon Co., Ltd.), acrylic resin (BR-77: manufactured by Mitsubishi Rayon Co., Ltd.) / Cellosolve acetate solution, benzoquinone, GOD (manufactured by Merck: 8 U / mg). The conductive inks b to f (Table 2) were mixed in an automatic mortar for 15 minutes, and the measurement electrode 3 was formed on the polyester substrate 2 by the screen printing method. The compounding ratio of carbon black / acrylic resin is 2
/ 1, and the absolute weight of both components in the measuring electrode is 90
% By weight. Further, GOD / benzoquinone was adjusted to 1/9 to 7/3 so that the absolute weight in the measuring electrode would be 10% by weight.
It was shaken at the compounding ratio of. A counter electrode 1 made of carbon was provided on the polyester substrate 2, and an insulating polymer layer 4 was provided while leaving a part of the electrode.
【００２５】作製した酵素電極を、実施例１と同様に評
価した。その結果、グルコース濃度０〜５００ｍｇ／ｄ
ｌの範囲で濃度に対応した応答が得られ（図３のｂ〜
ｆ）、特にＧＯＤ／ベンゾキノンが２／８〜６／４の配
合比の領域で高感度な応答性が観察された。The produced enzyme electrode was evaluated in the same manner as in Example 1. As a result, glucose concentration 0 to 500 mg / d
A response corresponding to the concentration was obtained in the range of 1 (b to b in FIG. 3).
Highly sensitive responsiveness was observed in f), particularly in the region of the blending ratio of GOD / benzoquinone of 2/8 to 6/4.
【００２６】＜比較例１＞実施例２と同様に、カーボン
ブラック（コロンビアカーボン社製）、アクリル樹脂
（ＢＲ−７７：三菱レイヨン（株）製）／酢酸セロソル
ブ溶液、ベンゾキノン、ＧＯＤ（メルク社製：８Ｕ／ｍ
ｇ）からなる導電性インキ（表２のａ、ｇ）を自動乳鉢
で１５分間混合し、スクリーン印刷法によりポリエステ
ル基板上に測定極３を形成した。カーボンブラック／ア
クリル樹脂は２／１、ＧＯＤ／ベンゾキノンは０．５／
９．５および８／２である。ポリエステル基板２には、
カーボンからなる対極１が設けてあり、電極の一部を残
して絶縁性のポリマー層４を設けた。Comparative Example 1 Carbon black (manufactured by Columbia Carbon Co., Ltd.), acrylic resin (BR-77: manufactured by Mitsubishi Rayon Co., Ltd.) / Cellosolve acetate solution, benzoquinone, GOD (manufactured by Merck Ltd.) as in Example 2. : 8 U / m
The conductive ink consisting of g) (a, g in Table 2) was mixed in an automatic mortar for 15 minutes, and the measurement electrode 3 was formed on the polyester substrate by the screen printing method. Carbon black / acrylic resin is 2/1, GOD / benzoquinone is 0.5 /
9.5 and 8/2. On the polyester substrate 2,
A counter electrode 1 made of carbon was provided, and an insulating polymer layer 4 was provided while leaving a part of the electrode.
【００２７】作製した酵素電極を、実施例１と同様に評
価した。その結果、グルコース濃度０〜５００ｍｇ／ｄ
ｌの範囲で、実施例２の結果と比較すると、電流値の応
答は大きく低下した（図３のａ、ｇ）。The prepared enzyme electrode was evaluated in the same manner as in Example 1. As a result, glucose concentration 0 to 500 mg / d
When compared with the results of Example 2 in the range of l, the response of the current value was significantly reduced (a, g in FIG. 3).
【００２８】[0028]
【表２】 [Table 2]
【００２９】[0029]
【発明の効果】以上のように、測定極中に占める酵素と
電子伝達物質を最適量に設定することで、応答性に優
れ、高感度な酵素電極を提供することができる。INDUSTRIAL APPLICABILITY As described above, by setting the enzyme and the electron transfer substance occupying in the measuring electrode to the optimum amounts, it is possible to provide an enzyme electrode having excellent responsiveness and high sensitivity.
【００３０】[0030]
【図１】本発明の一実施例に係る酵素電極の斜視図であ
る。FIG. 1 is a perspective view of an enzyme electrode according to an embodiment of the present invention.
【図２】実施例１におけるグルコース濃度と出力電流の
関係を示すグラフである。FIG. 2 is a graph showing the relationship between glucose concentration and output current in Example 1.
【図３】実施例２および比較例１におけるグルコース濃
度と出力電流の関係を示すグラフである。FIG. 3 is a graph showing the relationship between glucose concentration and output current in Example 2 and Comparative Example 1.
１…対極 ２…ポリエステル基板 ３…導電性インキか
らなる測定極 ４…絶縁性ポリマー層1 ... Counter electrode 2 ... Polyester substrate 3 ... Measuring electrode made of conductive ink 4 ... Insulating polymer layer
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 平７−151727（ＪＰ，Ａ) 特開 平７−270373（ＪＰ，Ａ) 特開 平７−77510（ＪＰ，Ａ) 特開 平６−90754（ＪＰ，Ａ) 特開 平６−78791（ＪＰ，Ａ) 特表 平３−503931（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 27/327 ─────────────────────────────────────────────────── ─── Continuation of the front page (56) Reference JP-A-7-151727 (JP, A) JP-A-7-270373 (JP, A) JP-A-7-77510 (JP, A) JP-A-6- 90754 (JP, A) JP-A-6-78791 (JP, A) Special Table 3-503931 (JP, A) (58) Fields investigated (Int.Cl. 7 , DB name) G01N 27/327
Claims (2)
及び電子伝達物質を含む導電性インキを、絶縁性基板上
に塗工後、加熱乾燥して形成した電極系において、酵素
と電子伝達物質の総量が、電極の１０〜４０重量％から
なることを特徴とする酵素電極。1. A total amount of an enzyme and an electron transfer substance in an electrode system formed by applying a conductive ink containing at least a conductive powder, a binder, an enzyme and an electron transfer substance onto an insulating substrate and then heating and drying. The enzyme electrode comprises 10 to 40 % by weight of the electrode.
量配合比が、１／９〜７／３であることを特徴とする請
求項１に記載の酵素電極。2. The enzyme electrode according to claim 1, wherein the weight ratio of the enzyme and the electron transfer substance in the enzyme electrode is 1/9 to 7/3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP06414794A JP3379202B2 (en) | 1994-03-31 | 1994-03-31 | Enzyme electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP06414794A JP3379202B2 (en) | 1994-03-31 | 1994-03-31 | Enzyme electrode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07270374A JPH07270374A (en) | 1995-10-20 |
| JP3379202B2 true JP3379202B2 (en) | 2003-02-24 |
Family
ID=13249683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP06414794A Expired - Fee Related JP3379202B2 (en) | 1994-03-31 | 1994-03-31 | Enzyme electrode |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3379202B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6565738B1 (en) * | 1999-01-28 | 2003-05-20 | Abbott Laboratories | Diagnostic test for the measurement of analyte in abiological fluid |
| US7588670B2 (en) | 2005-04-12 | 2009-09-15 | Lifescan Scotland Limited | Enzymatic electrochemical-based sensor |
| AU2006201333A1 (en) * | 2005-04-12 | 2006-11-02 | Lifescan Scotland Limited | Water-miscible conductive ink for use in enzymatic electrochemical-based sensors |
| US7465380B2 (en) | 2005-04-12 | 2008-12-16 | Lifescan Scotland, Ltd. | Water-miscible conductive ink for use in enzymatic electrochemical-based sensors |
-
1994
- 1994-03-31 JP JP06414794A patent/JP3379202B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07270374A (en) | 1995-10-20 |
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