JP3346727B2 - Biochip and biochip reader - Google Patents

Biochip and biochip reader

Info

Publication number
JP3346727B2
JP3346727B2 JP25480997A JP25480997A JP3346727B2 JP 3346727 B2 JP3346727 B2 JP 3346727B2 JP 25480997 A JP25480997 A JP 25480997A JP 25480997 A JP25480997 A JP 25480997A JP 3346727 B2 JP3346727 B2 JP 3346727B2
Authority
JP
Japan
Prior art keywords
biochip
spots
dna
fluorescent substance
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP25480997A
Other languages
Japanese (ja)
Other versions
JPH1194747A (en
Inventor
永典 奈須
顕次 山本
仁 藤宮
以子 百合野
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Software Engineering Co Ltd
Original Assignee
Hitachi Software Engineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Software Engineering Co Ltd filed Critical Hitachi Software Engineering Co Ltd
Priority to JP25480997A priority Critical patent/JP3346727B2/en
Publication of JPH1194747A publication Critical patent/JPH1194747A/en
Application granted granted Critical
Publication of JP3346727B2 publication Critical patent/JP3346727B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00531—Sheets essentially square
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00536—Sheets in the shape of disks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【０００１】[0001]

【発明の属する技術分野】本発明は分子生物学全般で使
用されているＤＮＡや蛋白質に蛍光物質を標識して、レ
ーザーで蛍光物質を励起し、蛍光の発光量でＤＮＡや蛋
白質の量を検出する読取り方式で、読取り装置が安価に
実現可能な手法に関する。バイオチップはＤＮＡや蛋白
質を高密度に集積して格納したものに関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for labeling DNA or protein used in general molecular biology with a fluorescent substance, exciting the fluorescent substance with a laser, and detecting the amount of DNA or protein by the amount of fluorescence emitted. in reading method of, reading device relates inexpensively <br/> feasible approach. The biochip relates to a device in which DNAs and proteins are stored at a high density.

【０００２】[0002]

【従来の技術】従来のバイオチップは四角形状で、図６
に示すようなスポットが植え付けてある。２はスポット
で、１７は蛍光標識されたＤＮＡや蛋白質で、１６は蛍
光物質である。バイオチップの読取りは、例えば図２に
示すようなものがある。図２において、１はバイオチッ
プで、２はバイオチップに蛍光物質が標識されたＤＮＡ
や蛋白質をスポットしたものであり、１のバイオチップ
を読取るために、５のレーザーを発信させ、６のミラー
でレーザー光を横軸に振り、２のスポットの蛍光物質を
励起させ、発光した蛍光を８の光ファイバーで集光し、
９の光学フィルタで目的の波長を抽出し、７のホトマル
で検出し、１のバイオチップを４の縦軸方向に駆動さ
せ、次のラインを読取り、これを繰り返してバイオチッ
プ全体を読取っていた。2. Description of the Related Art A conventional biochip has a quadrangular shape, and FIG.
Spots as shown in the figure are planted. 2 is a spot, 17 is a fluorescently labeled DNA or protein, and 16 is a fluorescent substance. The reading of the biochip is, for example, as shown in FIG. In FIG. 2, 1 is a biochip, and 2 is a DNA in which a fluorescent substance is labeled on the biochip.
In order to read 1 biochip, a laser 5 is emitted, and a laser beam is moved along the horizontal axis by a mirror 6 to excite the fluorescent substance in the spot 2 and emit fluorescence. With 8 optical fibers,
The target wavelength was extracted by the optical filter 9 and detected by the photomultiplier 7. The biochip 1 was driven in the direction of the vertical axis of 4, the next line was read, and this was repeated to read the entire biochip. .

【０００３】[0003]

【発明が解決しようとする課題】しかしながら、このよ
うな従来の方法では、バイオチップを読取るのにチップ
を駆動させるメカニズムとレーザーを振って読取るライ
ンセンサーのメカニズムが必要となり、コストが高くな
り問題となっていた。However, in such a conventional method, a mechanism for driving a chip and a mechanism for a line sensor for reading by shaking a laser are required for reading a biochip. Had become.

【０００４】本発明はチップの形状を円形状にし、読取
りは円盤を回転させ、ポイントセンサーで読取ること
で、低コストで読取りを可能とすることを目的とする。An object of the present invention is to make it possible to read at low cost by making a chip into a circular shape, reading by rotating a disk and reading by a point sensor.

【０００５】[0005]

【課題を解決するための手段】図１は本発明の原理説明
図である。本発明は１０のバイオチップを円形状に作成
し、１１のモータで回転させ、１８のポイントセンサ部
を、１９の円の中心方向に駆動させて読むことを特徴と
する。FIG. 1 is a diagram illustrating the principle of the present invention. The present invention is characterized in that 10 biochips are formed in a circular shape, rotated by 11 motors, and 18 point sensor units are driven in the direction of the center of 19 circles for reading.

【０００６】また、２のスポット内が化学発光で光って
いる光を読取るときは５のレーザーを切って読取り、蛍
光方式でも化学発光方式でも読取りができることを特徴
とする。[0006] Further, when reading light in which the inside of the spot 2 shines by chemiluminescence, reading is performed by turning off the laser 5 and reading can be performed by a fluorescent method or a chemiluminescent method.

【０００７】このような方式によれば、回転式でバイオ
チップを読取ることができ、センサーもポイントセンサ
ーであるため、ラインセンサーに比べ安価に装置の実現
ができる。バイオチップの読取装置が安価であれば各研
究室で手軽にバイオチップを使用した実験ができるよう
になり、遺伝子解明の研究のスピードアップに役立つ。[0007] According to such a system, the biochip can be read in a rotating manner, and since the sensor is also a point sensor, an apparatus can be realized at lower cost than a line sensor. If the biochip reader is inexpensive, it will be possible to easily carry out experiments using the biochip in each laboratory, which will help speed up research on gene elucidation.

【０００８】[0008]

【発明の実施の形態】以下、本発明の実施の形態を図面
に基づいて説明する。図６はスポット構造で、２はスポ
ット、１７は標識されたＤＮＡあるいは蛋白質、１６は
蛍光物質のTexasRedである。図３はスポットがトラック
単位に順次並んでいるバイオチップで、２はスポット、
１０はチップ全体である。チップの素材としてはガラス
を使用する。図４はスポットが同一角度で並んでいるバ
イオチップで、２はスポットで、１０はチップ全体であ
る。図５はスポットが渦巻きの形で並んでいるバイオチ
ップで、２はスポットで、１０は全体像である。図３、
図４及び図５はいずれも円盤型バイオチップの実施例を
示す図である。Embodiments of the present invention will be described below with reference to the drawings. FIG. 6 shows a spot structure, 2 is a spot, 17 is a labeled DNA or protein, and 16 is a fluorescent substance, TexasRed. FIG. 3 shows a biochip in which spots are sequentially arranged in track units.
10 is the whole chip. Glass is used as a chip material. FIG. 4 shows a biochip in which spots are arranged at the same angle, 2 is a spot, and 10 is the entire chip. FIG. 5 shows a biochip in which spots are arranged in a spiral, 2 is a spot, and 10 is a whole image. FIG.
4 and 5 are diagrams each showing an embodiment of a disc-shaped biochip.

【０００９】図１は本発明の一実施の形態に係わる全体
構成図である。図１において、１０は円盤型のバイオチ
ップであり、１１のモーターで回転させる。１８はポイ
ントセンサー部であり、５は半導体励起固体レーザーで
５３２ｎｍのレーザー光を発信させ、１３のダイクロイ
ックミラーでレーザー光の波長のみを２のスポットに向
けて反射させ、１２のレンズでレーザー光を絞って、２
のスポットに照射し、スポット中のDNAや蛋白質に標識
している蛍光物質を励起し、蛍光を発光させる。１２は
レンズで発光した蛍光を集光して平行光にし、１３のダ
イクロイックミラーを通り抜け、６２５ｎｍを選択的に
透過する光学干渉フィルター９で蛍光以外の光をカット
し、１５のレンズで絞り込み、７のホトマルへ光を入れ
て検出する。FIG. 1 is an overall configuration diagram according to an embodiment of the present invention. In FIG. 1, reference numeral 10 denotes a disk-shaped biochip, which is rotated by a motor 11. Reference numeral 18 denotes a point sensor unit. Reference numeral 5 denotes a semiconductor-excited solid-state laser that emits 532 nm laser light, 13 dichroic mirrors reflect only the wavelength of the laser light toward 2 spots, and 12 lenses transmit laser light. Squeeze 2
The spot is irradiated to excite a fluorescent substance labeled on DNA or protein in the spot to emit fluorescence. Numeral 12 converges the fluorescent light emitted by the lens into parallel light, passes through a dichroic mirror 13, cuts off light other than fluorescent light with an optical interference filter 9 that selectively transmits 625 nm, and narrows down with a lens 15. Light is injected into the photomultiplier to detect.

【００１０】なお、本発明において、化学発光でＤＮＡ
や蛋白質の標識を発光させたときは、５のレーザーを切
り、１４の光学干渉フィルターを発光波長を選択的に透
過する光学干渉フィルターに変更し読取れば、蛍光方式
と同様に、１０のバイオチップを読取ることができると
いう特長を有する。[0010] In the present invention, DNA is produced by chemiluminescence.
When the label of protein or protein is emitted, the laser of 5 is turned off, the optical interference filter of 14 is changed to an optical interference filter that selectively transmits the emission wavelength, and reading is performed. It has the feature that a chip can be read.

【００１１】更に、チップの両面にDNAなどをスポット
し、読み取ることにより、少ない検体の量で、効率的に
読み取ることが可能である。Further, by spotting and reading DNA or the like on both sides of the chip, it is possible to read efficiently with a small amount of sample.

【００１２】[0012]

【発明の効果】以上説明をしたように、本発明によれば
円盤型のバイオチップを回転して読めば、従来のレーザ
ー光を振って、光ファイバで集光して読む機構が不要と
なり、低コストで読取装置の実現ができる。As described above, according to the present invention, if a disk-shaped biochip is rotated and read, a conventional mechanism for shaking a laser beam and condensing it with an optical fiber to read it is unnecessary. A reading device can be realized at low cost.

【図面の簡単な説明】[Brief description of the drawings]

【図１】本発明の原理説明図である。FIG. 1 is a diagram illustrating the principle of the present invention.

【図２】従来の説明図である。FIG. 2 is a conventional explanatory diagram.

【図３】スポットがトラック単位に順次並んでいるバイ
オチップである。FIG. 3 is a biochip in which spots are sequentially arranged in track units.

【図４】スポットが同一角度で並んでいるバイオチップ
である。FIG. 4 is a biochip in which spots are arranged at the same angle.

【図５】スポットが渦巻きの形で並んでいるバイオチッ
プである。FIG. 5 is a biochip in which spots are arranged in a spiral.

【図６】スポットの構造である。FIG. 6 is a structure of a spot.

【符号の説明】[Explanation of symbols]

１…四角型バイオチップ、２…スポット、３…レーザー
光のスキャン方向、４…チップの駆動方向、５…レーザ
ー、６…振動ミラー、７…ホトマル、８…集光光ファイ
バー、９…光学フィルター、１０…円型バイオチップ、
１１…回転モーター、１２…集光レンズ、１３…ダイク
ロイックミラー、１５…ホトマル用集光レンズ、１６…
蛍光物質、１７…DNAまたは蛋白質、１８…ポイントセ
ンサー部、１９…ポイントセンサー部の駆動方向。DESCRIPTION OF SYMBOLS 1 ... Square biochip, 2 ... Spot, 3 ... Scanning direction of laser light, 4 ... Driving direction of chip, 5 ... Laser, 6 ... Vibrating mirror, 7 ... Photomaru, 8 ... Condensing optical fiber, 9 ... Optical filter, 10. Circular biochip,
11: rotating motor, 12: condensing lens, 13: dichroic mirror, 15: condensing lens for photomaru, 16 ...
Fluorescent substance, 17: DNA or protein, 18: Point sensor section, 19: Drive direction of the point sensor section.

───────────────────────────────────────────────────── フロントページの続き (72)発明者 藤宮 仁 神奈川県横浜市中区尾上町６丁目81番地 日立ソフトウェアエンジニアリング株 式会社内 (72)発明者 百合野 以子 神奈川県横浜市中区尾上町６丁目81番地 日立ソフトウェアエンジニアリング株 式会社内 (56)参考文献 特開 平４−315947（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 33/53 G01N 21/78 G01N 33/483 G01N 33/58 G01N 37/00 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Jin Fujimiya 6-81 Onoecho, Naka-ku, Yokohama-shi, Kanagawa Prefecture Within Hitachi Software Engineering Co., Ltd. 81 81 Hitachi Software Engineering Co., Ltd. (56) References JP-A-4-315947 (JP, A) (58) Fields investigated (Int. Cl. 7 , DB name) G01N 33/53 G01N 21/78 G01N 33/483 G01N 33/58 G01N 37/00

Claims (8)

(57)【特許請求の範囲】(57) [Claims] 【請求項１】蛍光物質で標識されたＤＮＡまたは蛋白質
から成る検体を格納する複数のスポットが、円盤状の表
面に径方向に向かって放射状に配列されていることを特
徴とするバイオチップ。1. DNA or protein labeled with a fluorescent substance
The spots containing specimens consisting of
A biochip characterized by being radially arranged on a surface in a radial direction . 【請求項２】蛍光物質で標識されたＤＮＡまたは蛋白質
から成る検体を格納する複数のスポットが、円盤状の表
面に螺旋状に配列されていることを特徴とするバイオチ
ップ。 2. DNA or protein labeled with a fluorescent substance
The spots containing specimens consisting of
Biochi characterized by being spirally arranged on the surface
Up. 【請求項３】蛍光物質で標識されたＤＮＡまたは蛋白質
から成る検体を格納する複数のスポットが、円盤状の表
面に同心円状に配列されているバイオチップと、前記各
スポットの検体にレーザー光を照射し、蛍光物質を励起
させ、蛍光発光量を検出する手段とを備えたことを特徴
とするバイオチップ読取り装置。3. A DNA or protein labeled with a fluorescent substance
The spots containing specimens consisting of
Biochips arranged concentrically on the surface,
Irradiate laser light to spot sample to excite fluorescent substance
Means for detecting the amount of fluorescence emission
Biochip reader . 【請求項４】 ＤＮＡまたは蛋白質から成る検体を格納す
る複数のスポットが、円盤状の表面に同心円状に配列さ
れているバイオチップと、前記各スポットの検体を化学
発光させ、その発光量を検出する手段とを備えたことを
特徴とするバイオチップ読取り装置。 4. A biochip in which a plurality of spots storing DNA or protein specimens are arranged concentrically on a disk-shaped surface, and the specimens in each of the spots are subjected to chemiluminescence, and the amount of luminescence is detected. A biochip reader. 【請求項５】 蛍光物質で標識されたＤＮＡまたは蛋白質
から成る検体を格納する複数のスポットが、円盤状の表
面に径方向に向かって放射状に配列されているバイオチ
ップと、前記各スポットの検体にレーザー光を照射し、
蛍光物質を励起させ、蛍光発光量を検出する手段とを備
えたことを特徴とするバイオチップ読取り装置。 5. A biochip in which a plurality of spots storing a DNA or protein sample labeled with a fluorescent substance are radially arranged on a disk-shaped surface in a radial direction, and a sample at each spot. Irradiate the laser light to
Means for exciting a fluorescent substance and detecting a fluorescence emission amount. 【請求項６】 ＤＮＡまたは蛋白質から成る検体を格納す
る複数のスポットが、円盤状の表面に径方向に向かって
放射状に配列されているバイオチップと、前記各スポッ
トの検体を化学発光させ、その発光量を検出する手段と
を備えたことを特徴とするバイオチップ読取り装置。 6. A biochip in which a plurality of spots storing DNA or protein specimens are radially arrayed on a disk-shaped surface in a radial direction, and the specimens in each of the spots are subjected to chemiluminescence. A biochip reader, comprising: means for detecting a light emission amount. 【請求項７】 蛍光物質で標識されたＤＮＡまたは蛋白質
から成る検体を格納する複数のスポットが、円盤状の表
面に螺旋状に配列されているバイオチップと、前記各ス
ポットの検体にレーザー光を照射し、蛍光物質を励起さ
せ、蛍光発光量を検出する手段とを備えたことを特徴と
するバイオチップ読取り装置。 7. A biochip in which a plurality of spots storing a DNA or protein sample labeled with a fluorescent substance are spirally arranged on a disk-shaped surface, and a laser beam is applied to each of the spot samples. Means for irradiating, exciting a fluorescent substance, and detecting the amount of fluorescent light emission. 【請求項８】 ＤＮＡまたは蛋白質から成る検体を格納す
る複数のスポットが、円盤状の表面に螺旋状に配列され
ているバイオチップと、前記各スポットの検体を化学発
光させ、その発光量を検出する手段とを備えたことを特
徴とするバイオチップ読取り装置。 8. A biochip in which a plurality of spots storing DNA or protein specimens are spirally arranged on a disk-shaped surface, and the specimens in each of the spots are subjected to chemiluminescence, and the amount of luminescence is detected. A biochip reader.
JP25480997A 1997-09-19 1997-09-19 Biochip and biochip reader Expired - Fee Related JP3346727B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP25480997A JP3346727B2 (en) 1997-09-19 1997-09-19 Biochip and biochip reader

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP25480997A JP3346727B2 (en) 1997-09-19 1997-09-19 Biochip and biochip reader

Publications (2)

Publication Number Publication Date
JPH1194747A JPH1194747A (en) 1999-04-09
JP3346727B2 true JP3346727B2 (en) 2002-11-18

Family

ID=17270195

Family Applications (1)

Application Number Title Priority Date Filing Date
JP25480997A Expired - Fee Related JP3346727B2 (en) 1997-09-19 1997-09-19 Biochip and biochip reader

Country Status (1)

Country Link
JP (1) JP3346727B2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3597729B2 (en) 1999-05-11 2004-12-08 日立ソフトウエアエンジニアリング株式会社 Fluorescence metering method and fluorescence meter
JP3942431B2 (en) * 1999-08-31 2007-07-11 三菱化学株式会社 Protein-molecule interaction analysis method
US7217573B1 (en) 1999-10-05 2007-05-15 Hitachi, Ltd. Method of inspecting a DNA chip
JP2001147230A (en) * 1999-11-19 2001-05-29 Hitachi Software Eng Co Ltd Biochip reading apparatus end labelled reagent
EP1679364A1 (en) * 2000-01-31 2006-07-12 Matsushita Ecology Systems Co., Ltd. Microorganism detecting kit, microorganism counting apparatus, and microorganism counting process
DE10013254A1 (en) 2000-03-17 2001-10-04 Friz Biochem Gmbh New apparatus, useful for detecting biomolecules and polymers, comprises array of samples containing specific molecules with separate light sources and system for detecting which samples interact with molecule to be detected
JP2002139499A (en) * 2000-08-23 2002-05-17 Sanyo Electric Co Ltd Chemical substance sensor and method for detecting chemical substance
EP1486786B1 (en) * 2002-03-15 2008-10-15 Sony Corporation Bio-assay substrate and method
JP2004093415A (en) * 2002-08-30 2004-03-25 Sony Corp Bioassay substrate, substrate information reading apparatus, and substrate information reading method
KR100529477B1 (en) * 2002-11-19 2005-11-22 재단법인서울대학교산학협력재단 CIS(CMOS Image Sensor) detecting method for detecting intensity of fluorescence light from a biochip
JP4388016B2 (en) 2003-01-31 2009-12-24 ユニバーサル・バイオ・リサーチ株式会社 Continuous optical measuring apparatus and method
JP2004257737A (en) * 2003-02-24 2004-09-16 Mitsui Eng & Shipbuild Co Ltd Biochip reader
JP2004264068A (en) * 2003-02-28 2004-09-24 Institute Of Tsukuba Liaison Co Ltd Bio-sensor chip
JP2005114427A (en) * 2003-10-03 2005-04-28 Sony Corp Method for manufacturing substrate for bioassay by superposing two substrates one upon another and substrate for bioassay
WO2005038053A1 (en) * 2003-10-18 2005-04-28 Nanostorage Co., Ltd. Biochip analysis system and diagnosis system having biochip analysis apparatus
KR100640153B1 (en) 2004-05-03 2006-10-31 (주)나노스토리지 Apparatus for patterning bio-cell
JP4285206B2 (en) * 2003-11-11 2009-06-24 ソニー株式会社 Bioassay substrate with extended power supply wiring
JP4300102B2 (en) 2003-12-05 2009-07-22 株式会社三菱化学ヤトロン Analyzer and collector
US20070247628A1 (en) 2004-09-10 2007-10-25 Wallac Oy Instrumentation and Method Adapted For Optical Measurement of an Amplified Luminescent Proximity Homogeneous Assay
JP4577645B2 (en) * 2004-09-30 2010-11-10 横河電機株式会社 Screening equipment
CN113308357A (en) * 2021-07-28 2021-08-27 中国农业大学 Intelligent anti-pollution PCR tube fluorescence detection system

Also Published As

Publication number Publication date
JPH1194747A (en) 1999-04-09

Similar Documents

Publication Publication Date Title
JP3346727B2 (en) Biochip and biochip reader
JP3597729B2 (en) Fluorescence metering method and fluorescence meter
US6207960B1 (en) System and methods for detection of labeled materials
JP2007033170A (en) Fluorescence detection method and fluorescence detection system
JP2002162351A (en) Method and apparatus for reading fluorescence
JP4152054B2 (en) Image reading device
JP3957118B2 (en) Test piece and image information reading device from the test piece
US20020028521A1 (en) Biochemical analyzing method, biochemical analysis apparatus, biochemical analysis unit used therefor and target detecting apparatus for detecting target from biochemical analysis unit
JP2001074656A (en) Image data reading apparatus
US6326628B1 (en) Image reading apparatus
JP3761726B2 (en) Microarray chip reading method and reading apparatus
JP2002156330A (en) Image data generation method and device
US6814298B2 (en) Digital data producing system
JP2002148265A (en) Biochemical analysis method, unit for biochemical analysis used therefor, and target detector for detecting target from unit for the biochemical analysis
JP2005241453A (en) Disk type biochip, bead reader, and bead reading system
JPH10221254A (en) Image reading device
JPH103135A (en) Image reader
JP2003185583A (en) Scanner
JP2002182323A (en) Focusing position determination method and focusing adjustment method for scanner having confocal optical system, scanner having the system, and focusing position determination device
JP2004037103A (en) Method for measuring dropping characteristics of spotting device
JP2004125605A (en) Scanner
JP2002156383A (en) Height position adjusting device, optical system height position adjusting device and data-generating device using optical system height position adjusting device
JP2000180362A (en) Image data reading method and apparatus
JP2004117272A (en) Method and device for reading data for biochemical analysis
JP2002168785A (en) Fixing method and device of sample stage

Legal Events

Date Code Title Description
R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20080906

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110906

Year of fee payment: 9

LAPS Cancellation because of no payment of annual fees