JP3263689B2 - Mushroom cultivation medium, mushroom cultivation method and nutrient additive for mushroom cultivation - Google Patents
Mushroom cultivation medium, mushroom cultivation method and nutrient additive for mushroom cultivationInfo
- Publication number
- JP3263689B2 JP3263689B2 JP36508799A JP36508799A JP3263689B2 JP 3263689 B2 JP3263689 B2 JP 3263689B2 JP 36508799 A JP36508799 A JP 36508799A JP 36508799 A JP36508799 A JP 36508799A JP 3263689 B2 JP3263689 B2 JP 3263689B2
- Authority
- JP
- Japan
- Prior art keywords
- medium
- powder
- granules
- shell
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Mushroom Cultivation (AREA)
Description
【０００１】[0001]
【発明の属する技術分野】この発明は、エノキタケやヒ
ラタケ等、鋸屑等の菌床培地で子実体形成可能なキノコ
の栽培用培地とキノコの栽培方法ならびにキノコ栽培用
栄養添加剤に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culture medium for cultivating mushrooms, a method for cultivating mushrooms, which can form fruiting bodies on a fungus bed medium such as enokitake and oyster mushroom, such as sawdust, and a nutrient additive for cultivating mushrooms.
【０００２】[0002]
【従来の技術】従来、キノコの人工的栽培方法として鋸
屑等の菌床培地に種菌を接種し、所定の温度及び湿度に
保って、子実体を形成させ、育成する方法が行われてい
た。2. Description of the Related Art Conventionally, as a method for artificially cultivating mushrooms, a method of inoculating a seed medium into a fungal bed medium such as sawdust and maintaining a predetermined temperature and humidity to form and grow fruiting bodies has been performed.
【０００３】一方、ここ数年間のキノコ類の年間総生産
額は２７００億円前後で推移し、頭打ち状態となってい
る。また、中国産シイタケに代表されるようにキノコ類
の輸入量が多くなってきたことも関連して、キノコ生産
は量とともに質も重視されるようになり、新たな付加価
値が付与されたキノコの栽培が、生産者、消費者ともに
望まれている。このような背景から、カルシウムを多量
に含む天然鉱物資源である貝化石に着目し、貝化石粉末
を培地に添加してキノコを栽培する方法が実用化さた。
これにより子実体収量は増加し、また子実体中のカルシ
ウム含有量は高くなり、高収量・高カルシウムのキノコ
栽培が可能となることが分かった。On the other hand, the annual total value of mushrooms in recent years has been around 270 billion yen, and has reached a plateau. In addition, imports of mushrooms, as represented by Chinese shiitake mushrooms, have increased, and mushroom production has become more important in terms of quantity and quality, and new value-added mushrooms have been added. The cultivation of is desired by both producers and consumers. From such a background, a method of cultivating mushrooms by adding shell fossil powder to a medium has been put to practical use, focusing on shell fossils, which are natural mineral resources containing a large amount of calcium.
As a result, it was found that the fruiting body yield was increased and the calcium content in the fruiting body was increased, so that high-yield and high-calcium mushroom cultivation became possible.
【０００４】[0004]
【発明が解決しようとする課題】上記従来の技術の場
合、貝化石粉末は微粒子であるため、培地調整の攪拌に
際してその投入時に粉末が飛散しやすいものであった。
このため、培地重量に対して適正な貝化石濃度にするこ
とが困難であり、加えて作業従事者の身体に付着して汚
れたり、さらには作業中に作業従事者がその粉末を吸入
するという健康上の問題もあった。In the prior art described above, since the shell fossil powder is a fine particle, the powder is liable to be scattered when the medium is stirred during the preparation of the medium.
For this reason, it is difficult to make the shell fossil concentration appropriate for the weight of the culture medium, and in addition, it adheres to the body of the worker and becomes dirty, and furthermore, the worker inhales the powder during the work. There were also health problems.
【０００５】この発明は上記従来の技術の問題点に鑑み
てなされたものであり、貝化石の取り扱いを容易にし、
さらに子実体収量及び子実体中のカルシウム含有量を高
めることができるキノコの栽培用培地とキノコの栽培方
法ならびにキノコ栽培用栄養添加剤を提供することを目
的とする。[0005] The present invention has been made in view of the above-mentioned problems of the prior art, and facilitates the handling of shell fossils.
It is another object of the present invention to provide a culture medium for cultivating mushrooms, a method for cultivating mushrooms, and a nutrient additive for cultivating mushrooms, which can increase the fruit body yield and the calcium content in the fruit body.
【０００６】[0006]
【課題を解決するための手段】この発明は、貝化石粉末
を直径１〜５ｍｍの顆粒状に成形し、この貝化石粉末顆
粒を培地に培地重量の０．３〜３％添加するキノコの栽
培用培地である。SUMMARY OF THE INVENTION The present invention provides a method for cultivating a mushroom in which shell fossil powder is formed into granules having a diameter of 1 to 5 mm, and the shell fossil powder granules are added to a culture medium in an amount of 0.3 to 3% by weight of the medium. It is a medium for use.
【０００７】また、貝化石粉末と、水溶性多糖類やこれ
を含む物質またはリグニン類とを８〜９．５：２〜０．
５（重量比）の割合で混合し、それら混合物を培地に、
培地重量の０．３〜３％添加するキノコの栽培用培地で
ある。[0007] Further, a fossil shellfish powder and a water-soluble polysaccharide, a substance containing the same or lignin are added in an amount of 8 to 9.5: 2 to 0.
5 (weight ratio), and mix them in a medium.
It is a culture medium for cultivating mushrooms to which 0.3 to 3% of the weight of the culture medium is added.
【０００８】また、貝化石粉末と水溶性多糖類やこれを
含む物質またはリグニン類とを８〜９．５：２〜０．５
（重量比）の割合で混合し、それら混合物を直径１〜５
ｍｍの顆粒状に成形し、この貝化石粉末顆粒を培地に培
地重量の０．３〜３％添加したキノコの栽培用培地であ
る。[0008] In addition, the shell fossil powder and the water-soluble polysaccharide or a substance or lignin containing the same may be mixed in an amount of 8-9.5: 2-0.5.
(Weight ratio) and mix the mixture to a diameter of 1-5.
This is a mushroom cultivation medium obtained by forming the shell fossil powder granules into a medium and adding 0.3 to 3% of the weight of the medium to the medium.
【０００９】また、貝化石粉末を直径１〜５ｍｍの顆粒
状に成形し、この貝化石粉末顆粒を培地に培地重量の
０．３〜３％添加してキノコの栽培用培地を調整し、こ
の栽培用培地に滅菌処理を施し、ここに種菌を接種して
適温適湿で培養し、子実体を発生させ成長させるキノコ
の栽培方法である。The shell fossil powder is formed into granules having a diameter of 1 to 5 mm, and the shell fossil powder granules are added to the medium in an amount of 0.3 to 3% of the weight of the medium to prepare a mushroom cultivation medium. This is a method of cultivating a mushroom in which a cultivation medium is sterilized, inoculated with a seed fungus, cultured at an appropriate temperature and an appropriate humidity to generate and grow fruit bodies.
【００１０】また、貝化石粉末と、水溶性多糖類やこれ
を含む物質またはリグニン類とを８〜９．５：２〜０．
５（重量比）の割合で混合し、それら混合物を培地に、
培地重量の０．３〜３％添加してキノコの栽培用培地を
調整し、この栽培用培地に滅菌処理を施し、ここに種菌
を接種して適温適湿で培養し、子実体を発生させ成長さ
せるキノコの栽培方法である。[0010] Further, shell fossil powder and a water-soluble polysaccharide, a substance containing the same or lignin are added in an amount of 8 to 9.5: 2 to 0.
5 (weight ratio), and mix them in a medium.
A culture medium for cultivating mushrooms is prepared by adding 0.3 to 3% of the weight of the culture medium, the culture medium is sterilized, inoculated with an inoculum, and cultured at an appropriate temperature and humidity to generate fruit bodies. This is a method of growing mushrooms.
【００１１】また、貝化石粉末と、水溶性多糖類やこれ
を含む物質またはリグニン類とを８〜９．５：２〜０．
５（重量比）の割合で混合し、それら混合物を直径１〜
５ｍｍの顆粒状に成形し、この貝化石粉末顆粒を培地に
培地重量の０．３〜３％添加してキノコの栽培用培地を
調整し、この栽培用培地に滅菌処理を施し、ここに種菌
を接種して適温適湿で培養し、子実体を発生させ成長さ
せるキノコの栽培方法である。Further, the fossil shell powder and the water-soluble polysaccharide, a substance containing the polysaccharide or lignin are 8-9.5: 2-0.
5 (weight ratio), and the mixture was mixed with a diameter of 1 to 5.
The mushroom cultivation medium is prepared by adding the shell fossil powder granules to the culture medium in an amount of 0.3 to 3% of the weight of the medium, and the cultivation medium is sterilized. This is a method for cultivating mushrooms which are inoculated and cultured at appropriate temperature and humidity to generate and grow fruiting bodies.
【００１２】また、貝化石粉末と、水溶性多糖類または
リグニン類とを８〜９．５：２〜０．５（重量比）の割
合で混合したキノコ栽培用栄養添加剤である。さらに、
それら混合物を直径１〜５ｍｍの顆粒状に成形したキノ
コ栽培用栄養添加剤である。Further, the present invention is a nutrient additive for cultivating mushrooms, wherein fossil shell powder and water-soluble polysaccharide or lignin are mixed at a ratio of 8 to 9.5: 2 to 0.5 (weight ratio). further,
It is a nutrient additive for mushroom cultivation obtained by molding the mixture into granules having a diameter of 1 to 5 mm.
【００１３】ここで、上記水溶性多糖類やこれを含む物
質は、でんぷん、デキストリン、カラマツ水抽出物、コ
ンニャク粉、小麦粉、糖蜜または廃糖蜜である。Here, the water-soluble polysaccharide and the substance containing the same are starch, dextrin, larch water extract, konjac flour, flour, molasses or molasses.
【００１４】この発明のキノコの栽培用培地とキノコの
栽培方法ならびにキノコ栽培用栄養添加剤は、貝化石粉
末が多孔質な顆粒状に成形されることにより、貝化石を
粉末状で培地に添加した場合に比べて、空隙を多く確保
する事ができるので好気性のキノコ菌糸体にとって成長
しやすい培地環境となる。さらに、貝化石粉末を顆粒状
に成形するためのバインダーは、粘着性を有し、なおか
つキノコ菌糸体の成長に良好な作用を示すデキストリ
ン、でんぷん、カラマツ水抽出物等の水溶性多糖類やリ
グニンスルホン酸塩等を選定したことにより、培地中に
おいてバインダー成分と貝化石成分との相乗作用により
菌糸体の成長が促進され、貝化石中のカルシウム成分が
菌糸体に吸収されやすくなり、子実体収量が増加し、子
実体中のカルシウム含有量も高くなるものである。The cultivation medium for mushrooms, the method for cultivating mushrooms, and the nutrient additive for cultivating mushrooms according to the present invention are characterized in that shell fossil powder is formed into porous granules, whereby shell fossils are added to the medium in powder form. As compared with the case where the aerobic mushroom mycelium is formed, a medium environment in which the aerobic mushroom mycelium can easily grow is provided. Further, a binder for forming shell fossil powder into granules is a water-soluble polysaccharide or lignin such as dextrin, starch, larch water extract, which is sticky and has a good effect on the growth of mushroom mycelium. By selecting a sulfonate, etc., the growth of the mycelium is promoted by the synergistic action of the binder component and the shell fossil component in the medium, the calcium component in the shell fossil is easily absorbed by the mycelium, and the fruiting body yield And the calcium content in the fruit body also increases.
【００１５】[0015]
【発明の実施の形態】以下、この発明の実施形態につい
て説明する。この発明の一実施形態のキノコ栽培用栄養
添加剤は、ほぼ直径１ｍｍ以下の貝化石粉末とバインダ
ーとを、８〜９．５：２〜０．５（重量比）の割合で混
合したものであり、バインダーとしては、デキストリ
ン、でんぷん、カラマツ水抽出物等の水溶性多糖類また
はリグニンスルホン酸塩等のリグニン類である。そし
て、それら混合物を、直径１〜５ｍｍの顆粒状に成形し
た貝化石粉末顆粒である。Embodiments of the present invention will be described below. The nutrient additive for mushroom cultivation according to one embodiment of the present invention is obtained by mixing a fossil shell powder having a diameter of about 1 mm or less and a binder in a ratio of 8 to 9.5: 2 to 0.5 (weight ratio). The binder is a water-soluble polysaccharide such as dextrin, starch, larch water extract, or a lignin such as lignin sulfonate. And these mixtures are shell fossil powder granules formed into granules having a diameter of 1 to 5 mm.
【００１６】次に、キノコの栽培用培地の調整について
説明する。まず、基本培地は、培地基材のエゾマツオガ
コ等と米糠等の栄養材を所定重量比で混合し、水分調整
したものである。この基本培地に、この実施形態のキノ
コ栽培用栄養添加剤を、基本培地重量に対して０．３〜
３％で添加し、栽培用培地とする。Next, the preparation of a culture medium for mushrooms will be described. First, the basic medium is prepared by mixing nutrients such as rice bran and the like, which are base materials of the medium, with a predetermined weight ratio and adjusting the water content. In this basal medium, the nutrient additive for mushroom cultivation of this embodiment is added in an amount of 0.3 to
It is added at 3% to obtain a culture medium.
【００１７】次に、この実施形態のキノコの栽培方法に
ついて説明する。まず、この栽培用培地をビンに詰め、
この培地をビンごと滅菌処理する。そして、この培地に
エノキタケ等のキノコの種菌を接種し、一定期間所定温
度下で培養する。そして、菌掻き後、芽出し処理を行
い、所定温度で培養を続けると子実体が発生し、傘の開
きが適当な状態になったとき子実体を収穫する。Next, a method for cultivating mushrooms according to this embodiment will be described. First, pack this culture medium into bottles,
The medium is sterilized with the bottle. Then, this medium is inoculated with a seed fungus of mushrooms such as enokitake mushroom and cultured at a predetermined temperature for a certain period of time. Then, after scraping the bacteria, a sprout treatment is performed, and when the culture is continued at a predetermined temperature, fruiting bodies are generated. When the opening of the umbrella is in an appropriate state, the fruiting bodies are harvested.
【００１８】この実施形態のキノコの栽培用培地とキノ
コの栽培方法ならびにキノコ栽培用栄養添加剤によれ
ば、貝化石粉末が多孔質な顆粒状に成形されることによ
り、貝化石を粉末状で培地に添加した場合に比べて、空
隙を多く確保する事ができるので好気性のキノコ菌糸体
にとって成長しやすい培地環境となる。さらに、貝化石
粉末を顆粒状に成形するためのバインダーは、粘着性を
有し、なおかつキノコ菌糸体の成長に良好な作用を示す
デキストリン、でんぷん、カラマツ水抽出物等の高分子
多糖類やリグニンスルホン酸塩を選定したことにより、
培地中においてバインダー成分と貝化石成分との相乗作
用により菌糸体の成長が促進され、貝化石中のカルシウ
ム成分が菌糸体に吸収されやすくなり、子実体収量が増
加し、さらに食感も良好なキノコとなる。そして、子実
体中のカルシウム含有量が高くなり、健康志向に対応し
た新たな付加価値が付与される。According to the mushroom cultivation medium and the mushroom cultivation method and the nutrient additive for mushroom cultivation of this embodiment, the shell fossil powder is formed into a porous granule, so that the shell fossil is converted into powder. As compared with the case where the medium is added to the medium, more voids can be secured, so that a medium environment in which the aerobic mushroom mycelium can grow easily is provided. Further, the binder for forming the shell fossil powder into granules has a high viscosity, and also has a good effect on the growth of mushroom mycelia, such as dextrin, starch, and high molecular weight polysaccharides such as larch water extract and lignin. By selecting the sulfonate,
The growth of mycelium is promoted by the synergistic action of the binder component and the shell fossil component in the medium, the calcium component in the shell fossil is easily absorbed by the mycelium, the fruiting body yield is increased, and the texture is also good. Become a mushroom. Then, the calcium content in the fruit body increases, and new added value corresponding to the health consciousness is provided.
【００１９】したがって、キノコの栽培用培地とキノコ
の栽培方法ならびにキノコ栽培用栄養添加剤の貝化石粉
末顆粒は、上記リグニンや水溶性多糖類を含まない貝化
石粉末の顆粒であっても上記キノコの増収作用がある。
同様に、貝化石粉末を顆粒状とはせずに、単に上記リグ
ニンや水溶性多糖類を貝化石粉末に添加したものを培地
基材に混合したキノコ栽培用培地であっても、キノコの
増収が可能である。Therefore, the shell fossil powder granules of the mushroom cultivation medium and the mushroom cultivation method and the nutrient additive for mushroom cultivation may be the shell fossil powder granules not containing the lignin or the water-soluble polysaccharide. Has the effect of increasing sales.
Similarly, even if the mushroom cultivation medium in which the lignin or the water-soluble polysaccharide is added to the shell fossil powder without mixing the shell fossil powder into the granular form and mixed with the culture medium base, the increase in mushrooms Is possible.
【００２０】また、この実施形態によれば、消費者の健
康志向に対応した付加価値をキノコに付与することがで
き、新たな需要が期待でき、キノコ生産者の経営安定化
にもつながるものである。さらに貝化石資材の製造業界
においては、貝化石資材の用途拡大が図られ、業界の振
興に寄与する。Further, according to this embodiment, it is possible to add a value added to a mushroom corresponding to a consumer's health consciousness, expect a new demand, and lead to a stable management of a mushroom producer. is there. Furthermore, in the shell fossil material manufacturing industry, the use of shell fossil materials will be expanded, contributing to the promotion of the industry.
【００２１】なお、この発明のキノコの栽培用培地とキ
ノコの栽培方法ならびにキノコ栽培用栄養添加剤は、エ
ノキタケ以外にもナメコ、シイタケ、ヒラタケ、ブナシ
メジ、マイタケ等、鋸屑等の菌床培地で子実体形成可能
な他のキノコ栽培にも適用可能である。また菌床培地は
鋸屑以外にコーン等の材料を用いても良い。The cultivation medium for mushrooms, the method for cultivating mushrooms, and the nutritional additive for cultivating mushrooms according to the present invention are not limited to enokitake mushrooms, but may be used in the culture medium of sawdust, such as nameko, shiitake mushroom, oyster mushroom, bunashimeji, maitake, etc. It can be applied to other mushroom cultivation that can form a substance. In addition, a material such as corn may be used for the culture medium other than sawdust.
【００２２】[0022]
【実施例】以下この発明の実施例について説明する。先
ず、貝化石粉末の添加量と、キノコの子実体収量及び子
実体中のカルシウム含有量の関係を調べる実験を行っ
た。基本培地は、培地基材のエゾマツオガコと栄養剤の
米糠を、培地基材と栄養剤を１８：１７（重量比）で混
合し、含水率が湿量基準で６５％になるように水道水を
加え調湿し、これを基本培地とする。Embodiments of the present invention will be described below. First, an experiment was conducted to examine the relationship between the amount of shell fossil powder added, the fruit body yield of mushrooms, and the calcium content in the fruit body. The basal medium is a mixture of the medium base, Ezomatsu sawdust, and the rice bran, a nutrient, in a ratio of 18:17 (weight ratio) of the medium base and the nutrient, and tap water so that the water content is 65% on a wet basis. And adjust the humidity, and use this as the basal medium.
【００２３】次にこの基本培地に、貝化石粉末を表１に
示すように各々所定の添加量を混入し、供試培地とす
る。各々の供試培地をポリプロピレン製８５０ｍｌビン
に５２０ｇ詰め、１２０℃で４５分間滅菌処理し、放冷
後、エノキタケ種菌を接種した。その後、１６±２℃で
２９日間培養し、菌掻き後、芽出し処理を１４±２℃で
１１日間、抑制処理を５±２℃で８日間、生育処理を７
±２℃で１４日間とし、その後、子実体を収穫し、生重
量を測定して子実体収量とした。Next, as shown in Table 1, a predetermined amount of each of the shell fossil powders is mixed into the basic medium to prepare a test medium. 520 g of each test medium was packed in a polypropylene 850 ml bottle, sterilized at 120 ° C. for 45 minutes, allowed to cool, and inoculated with Enokitake mushrooms. Thereafter, the cells were cultured at 16 ± 2 ° C. for 29 days. After scraping the bacteria, the sprouting treatment was carried out at 14 ± 2 ° C. for 11 days, the suppression treatment was carried out at 5 ± 2 ° C. for 8 days, and the growth treatment was carried out for 7 days.
After 14 days at ± 2 ° C, fruiting bodies were harvested and fresh weight was measured to obtain fruiting body yield.
【００２４】子実体収量を測定した後、石付き部（根元
から３ｃｍ）を廃棄したエノキタケ子実体を凍結乾燥処
理し、３０メッシュ以下に粉砕してカルシウム含有量測
定用の試料とした。まず、所定量の試料を秤量し、テフ
ロン製加圧分解容器で１７０℃で５時間の条件で濃硝酸
に完全に溶解し、ＩＣＰ発光分析計にてカルシウム含有
量を定量した。After measuring the fruiting body yield, the enokitake mushroom fruiting body whose stone attached portion (3 cm from the root) was discarded was freeze-dried and pulverized to 30 mesh or less to prepare a sample for calcium content measurement. First, a predetermined amount of a sample was weighed, completely dissolved in concentrated nitric acid at 170 ° C. for 5 hours in a Teflon pressure decomposition vessel, and the calcium content was quantified by an ICP emission spectrometer.
【００２５】なお、基本培地のみを無添加区とし、同様
にエノキタケの栽培を行い、子実体収量とカルシウム含
有量を測定した。[0025] Enokitake was cultivated in the same manner except that only the basal medium was added, and the fruiting body yield and calcium content were measured.
【００２６】上記の子実体収量の測定結果を表１に示
す。Table 1 shows the results of the measurement of the fruiting body yield.
【表１】 [Table 1]
【００２７】この結果、基本培地に貝化石粉末を混合す
ることにより、０．３〜２％添加することにより子実体
収量は増加した。特に、添加量を、基本培地に対して
２．０％添加した場合に最も子実体収量が多く、無添加
区に対して約２０％増加した。As a result, the yield of fruiting bodies was increased by adding 0.3 to 2% by mixing the shell fossil powder with the basal medium. In particular, when 2.0% was added to the basal medium, the fruiting body yield was the highest, and the yield was increased by about 20% compared to the non-added group.
【００２８】次に、上記エノキタケの子実体中のカルシ
ウム含有量を表２に示す。Next, Table 2 shows the calcium content in the fruit body of the enokitake mushroom.
【表２】 [Table 2]
【００２９】この結果、基本培地に貝化石粉末を混合す
ることにより、子実体中のカルシウム含有量は増加し、
貝化石粉末添加量に比例してカルシウム含有量も増加し
た。特に、１．５％以上添加した場合に、カルシウム含
有量が高い値を示し、無添加区に対して８倍以上増加し
た。As a result, the calcium content in the fruiting body is increased by mixing the shell medium powder with the basal medium,
The calcium content also increased in proportion to the amount of shell fossil powder added. In particular, when 1.5% or more was added, the calcium content showed a high value, and increased 8 times or more as compared with the non-added group.
【００３０】以上のことから、エノキタケの栽培におい
て貝化石粉末の添加量は基本培地に対して２．０％程度
が好ましく、確実に子実体収量と子実体中のカルシウム
含有量が多くなることがわかった。From the above, in the cultivation of Enokitake mushroom, the addition amount of the shell fossil powder is preferably about 2.0% based on the basic medium, and the fruiting body yield and the calcium content in the fruiting body are surely increased. all right.
【００３１】次に、上記と同様に、貝化石粉末の添加量
と、キノコの子実体収量及び子実体中のカルシウム含有
量の関係を調べる実験を、ナメコについて行った。この
子実体収量の測定結果を表３に示す。Next, in the same manner as described above, an experiment was conducted on nameko to examine the relationship between the amount of added fossil shell powder, the fruit body yield of mushrooms, and the calcium content in the fruit body. Table 3 shows the measurement results of the fruiting body yield.
【００３２】[0032]
【表３】 [Table 3]
【００３３】この結果、基本培地に貝化石粉末を混合す
ることにより、子実体収量は増加した。添加量が０．１
％〜０．４％の場合、添加量の増加に伴い子実体収量が
増加する。また、０．４〜１．２％および２．０％の添
加量では、子実体収量は上記０．４％の添加量の場合と
同程度の収量増加であり、添加量が１．６％の場合に最
も子実体収量が増加し、無添加区に対して約５０％の収
量増加を示した。As a result, the fruiting body yield was increased by mixing the shell fossil powder with the basal medium. 0.1
% To 0.4%, the fruiting body yield increases as the amount of addition increases. In addition, at the addition amounts of 0.4 to 1.2% and 2.0%, the fruiting body yield is about the same increase in yield as the case of the addition amount of 0.4%, and the addition amount is 1.6%. In the case of (1), the yield of the fruiting body increased most, and the yield increased by about 50% with respect to the non-added group.
【００３４】次に、上記ナメコの子実体中のカルシウム
含有量を表４に示す。Next, Table 4 shows the calcium content in the fruit body of the above nameko.
【表４】 [Table 4]
【００３５】この結果、基本培地に貝化石粉末を混合す
ることにより、子実体中のカルシウム含有量は増加し
た。貝化石粉末の添加量の増加に伴い、子実体中のカル
シウム含有量は増加し、特に添加量２．０％で、無添加
区の２，５２倍と高い値を示した。As a result, the calcium content in the fruit body was increased by mixing the fossil shell powder with the basal medium. The calcium content in the fruiting body increased with the increase in the amount of shell fossil powder added. In particular, the addition amount was 2.0%, which was a high value of 2,52 times that of the non-addition group.
【００３６】このことから、ナメコの栽培においても、
エノキタケと同様に貝化石粉末の添加量は基本培地に対
して２．０％程度が好ましく、確実に子実体収量と子実
体中のカルシウム含有量が多くなることがわかった。[0036] From this, even in the cultivation of nameko,
Like the enokitake mushroom, the addition amount of the shell fossil powder is preferably about 2.0% based on the basal medium, and it was found that the fruiting body yield and the calcium content in the fruiting body surely increased.
【００３７】次に、この発明の一実施例について説明す
る。この実施例ではエノキタケを栽培した。まず、この
実施例のキノコ栽培用栄養添加剤は、貝化石粉末とデキ
ストリンを８：２（重量比）の割合で混合したもの、貝
化石粉末とでんぷんを８：２（重量比）の割合で混合し
たもの、貝化石粉末とリグニンスルホン酸塩を８：２
（重量比）の割合で混合したもの、貝化石粉末とカラマ
ツ水抽出物（以下ＫＷＥ）を９：１（重量比）の割合で
混合したものを各々顆粒状に成形した。粒の直径は３種
類とし、直径１〜２ｍｍのＡ型、直径２〜３．５ｍｍの
Ｂ型、直径３．５〜５ｍｍのＣ型を作った。Next, an embodiment of the present invention will be described. In this example, enokitake was cultivated. First, the nutrient additive for mushroom cultivation of this example is a mixture of fossil shell powder and dextrin in a ratio of 8: 2 (weight ratio), and a mixture of fossil shell powder and starch in a ratio of 8: 2 (weight ratio). 8: 2 mixture of fossil shellfish powder and lignin sulfonate
(Weight ratio), and a mixture of shell fossil powder and larch water extract (KWE) at a ratio of 9: 1 (weight ratio) were each formed into granules. The diameters of the grains were three types, and A type having a diameter of 1 to 2 mm, B type having a diameter of 2 to 3.5 mm, and C type having a diameter of 3.5 to 5 mm were produced.
【００３８】次に、キノコの栽培用培地について説明す
る。まず、基本培地は、上記と同様に、培地基材のエゾ
マツオガコと栄養材の米糠を、１８：１７（重量比）で
混合し、含水率が湿量基準で６５％になるように水道水
を加え調湿したものである。この基本培地に、上記の各
キノコ栽培用栄養添加剤を基本培地重量に対して２％添
加した。これは、上述の通り貝化石粉末を基本培地重量
に対して２％添加することが好ましいからである。Next, a culture medium for cultivating mushrooms will be described. First, in the same manner as described above, the basal medium was prepared by mixing Ezomatsu octopus as a medium base material and rice bran as a nutrient at a ratio of 18:17 (weight ratio), and using tap water such that the water content was 65% on a wet basis. It was humidified. Each of the above-mentioned nutrient additives for mushroom cultivation was added to this basic medium at 2% with respect to the weight of the basic medium. This is because, as described above, it is preferable to add 2% of the shell fossil powder to the weight of the basic medium.
【００３９】そして、上記エノキタケの栽培例と同様の
キノコの栽培方法を行った。その後、子実体を収穫し、
生重量を測定して子実体収量とした。また、同様に子実
体のカルシウム含有量を定量した。Then, the same method for cultivating mushrooms as in the cultivation example of Enokitake mushroom was performed. After that, harvest the fruiting body,
The fresh weight was measured and defined as the fruiting body yield. Similarly, the calcium content of the fruiting body was determined.
【００４０】なお、この実施例において、基本培地のみ
からなる培地を無添加区とした。その他に、基本培地
に、貝化石粉末を基本培地重量に対して２％添加した試
験区を対照区とし、また、上記のキノコ栽培用栄養添加
剤に含有されている各バインダー量を、バインダー単独
で基本培地に添加したものを単独区とした。そして、上
記キノコ栽培用栄養添加剤を顆粒状に成形したことの影
響を比較するため、貝化石粉末（Ｆｓ）とデキストリ
ン、貝化石粉末（Ｆｓ）とでんぷん、貝化石粉末（Ｆ
ｓ）とリグニンスルホン酸塩を、各々を８：２（重量
比）で混合した混合粉末と、貝化石粉末（Ｆｓ）とＫＷ
Ｅを９：１（重量比）で混合した混合粉末を、上記の基
本培地に培地重量に対して２％添加したものを混合区と
した。対照区、単独区、混合区とも、同様にエノキタケ
の栽培を行い、子実体収量とカルシウム含有量を測定し
た。In this example, a medium consisting only of a basal medium was used as a non-added section. In addition, a control plot in which shell fossil powder was added to the basic culture medium at 2% of the weight of the basic culture medium was used as a control plot, and the amount of each binder contained in the above-mentioned nutrient additive for mushroom cultivation was determined using the binder alone. , A single medium was added to the basal medium. Then, in order to compare the effect of forming the nutrient additive for mushroom cultivation into granules, shell fossil powder (Fs) and dextrin, shell fossil powder (Fs) and starch, shell fossil powder (F)
s) and lignin sulfonate in a ratio of 8: 2 (weight ratio), shell fossil powder (Fs) and KW
E was mixed at a ratio of 9: 1 (weight ratio) to the above-mentioned basic medium, and 2% based on the weight of the medium was added to the above-mentioned basic medium to obtain a mixed group. Enokitake mushrooms were similarly cultivated in the control plot, the single plot and the mixed plot, and the fruiting body yield and calcium content were measured.
【００４１】上記エノキタケの子実体収量の測定結果を
以下の表に示す。The results of the measurement of the fruiting body yield of the above enokitake are shown in the following table.
【表５】 [Table 5]
【００４２】この結果、無添加区での子実体収量は約１
３３．７ｇ／ビンとなり、対照区での子実体収量は約１
４５．１ｇ／ビンとなり、対照区では貝化石粉末の添加
により基本培地に比べて子実体収量は約１０％増加し
た。このことからも、貝化石粉末のみを添加してもある
程度の効果があることがわかった。As a result, the fruit body yield in the non-added group was about 1
33.7 g / bin, and the fruiting body yield in the control group was about 1
The yield was 45.1 g / bin. In the control group, the addition of the shell fossil powder increased the fruiting body yield by about 10% compared to the basal medium. From this, it was found that the addition of only the shell fossil powder had a certain effect.
【００４３】また、バインダーのみを添加した単独区で
は、リグニンは対照区と同程度の子実体収量を示した
が、その他のＫＷＥ、でんぷん、デキストリンでは、子
実体収量は対照区に比べて５〜１０％減少した。In the single group to which only the binder was added, lignin showed the same fruit body yield as the control group, but the other KWE, starch, and dextrin showed the fruit body yield of 5 to 5 compared with the control group. It decreased by 10%.
【００４４】そして、貝化石粉末と各々のバインダーを
混合粉末の状態で添加した混合区では、Ｆｓ＋リグニ
ン、Ｆｓ＋でんぷんにおいては、対照区や、単独区のリ
グニンと同等の子実体収量を示したが、Ｆｓ＋ＫＷＥ、
Ｆｓ＋デキストリンでは、対照区や、単独区のＫＷＥと
デキストリンよりも子実体収量は多くなった。このこと
から、ＫＷＥやデキストリンは、子実体収量に対して貝
化石成分との相乗効果が認められた。In the mixed group in which the shell fossil powder and the respective binders were added in the form of a mixed powder, Fs + lignin and Fs + starch showed the same fruit body yield as the lignin in the control group and the lignin in the single group. , Fs + KWE,
In the case of Fs + dextrin, the fruiting body yield was higher than those of KWE and dextrin in the control section and the single section. From this, it was confirmed that KWE and dextrin had a synergistic effect with the shellfish fossil component on the fruit body yield.
【００４５】また、貝化石粉末と各々のバインダーを混
合し、顆粒状に形成して添加した顆粒状区では、リグニ
ンにおいて、リグニンＢ型、リグニンＣ型は、対照区、
単独区のリグニン、混合区のＦｓ＋リグニンよりも子実
体収量は多くなった。顆粒状区のでんぷんにおいて、で
んぷんＡ型、でんぷんＢ型、でんぷんＣ型は、対照区、
単独区のでんぷん、混合区のＦｓ＋でんぷんよりも子実
体収量は多くなった。また、顆粒状区のデキストリンに
おいて、デキストリンＢ型は、対照区、単独区のデキス
トリン、混合区のＦｓ＋デキストリンよりも子実体収量
は多くなり、デキストリンＡ型は混合区のＦｓ＋デキス
トリンと、またデキストリンＣ型は対照区と同程度の子
実体収量となった。このように、顆粒状区のリグニン、
でんぷん、デキストリンは、混合区や対照区よりも子実
体収量は多くなり、貝化石成分とバインダーとの相乗効
果を一層強くする作用が認められた。そして、顆粒状区
のＫＷＥにおいて、ＫＷＥ・Ｂ型は混合区のＦｓ＋ＫＷ
Ｅと同程度、またＫＷＥ・Ａ型は対照区と同程度、ＫＷ
Ｅ・Ｃ型は単独区のＫＷＥと同程度の子実体収量を示し
た。[0045] In the granular section in which the shell fossil powder and each binder were mixed and formed into granules and added, lignin B type and lignin C type were compared with the control section.
The fruiting body yield was higher than the lignin in the single group and the Fs + lignin in the mixed group. In the granular starch, starch A type, starch B type, starch C type is a control group,
The fruiting body yield was higher than the starch in the single group and the Fs + starch in the mixed group. In the dextrin in the granular section, the dextrin type B had a higher fruiting body yield than the control section, the dextrin in the single section, and the Fs + dextrin in the mixed section. The fruit type yield was similar to that of the control. Thus, lignin in the granular compartment,
Starch and dextrin showed higher fruit body yields than the mixed and control plots, and the synergistic effect between the shellfish fossil component and the binder was further enhanced. And in KWE of granular section, KWE-B type is Fs + KW of mixed section.
Equivalent to E, KWE / A type similar to control, KW
The EC type showed the same fruiting body yield as KWE in the single plot.
【００４６】したがって、この実施例のキノコ栽培用栄
養添加剤を添加したものは、貝化石粉末とバインダーを
混合粉末の状態で添加したものや貝化石粉末のみを添加
したものと同等あるいはそれ以上の子実体収量を示すこ
とがわかった。また、粒径別による傾向は、Ｂ型がＡ
型、Ｃ型に比べて子実体収量が多くなる傾向を示した。Therefore, the addition of the nutrient additive for mushroom cultivation of this embodiment is equivalent to or more than the addition of the shell fossil powder and the binder in a mixed powder state or the addition of the shell fossil powder alone. It was found to show fruiting body yield. In addition, the tendency according to the particle size is as follows.
There was a tendency that the fruiting body yield was higher than that of the C and C types.
【００４７】次に、上記エノキタケの子実体中のカルシ
ウム含有量を表６に示す。Next, Table 6 shows the calcium content in the fruit body of the enokitake mushroom.
【表６】 [Table 6]
【００４８】この結果、無添加区では１８．５８ｍｇ／
１００ｇ乾物重量となり、対照区は無添加区に対して２
５％増化した２３．２６ｍｇ／１００ｇ乾物重量となっ
た。また、単独区では、いずれも対照区よりも約２０％
少ないカルシウム含有量を示し、無添加区と同程度のカ
ルシウム含有量となった。As a result, 18.58 mg /
100 g dry weight, control group was 2
It was 23.26 mg / 100 g dry matter weight increased by 5%. In addition, in the single plot, all were about 20% higher than in the control plot.
The calcium content was low, and was about the same as that of the non-added group.
【００４９】また、混合区ではいずれも無添加区や単独
区に比べて多くなった。しかし、対照区よりも減少し、
これは培地中の貝化石成分の濃度が対照区の８０〜９０
％であるためである。In addition, in each of the mixed plots, the number was larger than in the non-added plots and the single plots. However, it decreased from the control plot,
This means that the concentration of the shellfish fossil component in the medium was 80-90 of the control group.
%.
【００５０】また、顆粒状区では、リグニンにおいてリ
グニンＡ型、Ｂ型は対照区よりカルシウム含有量はやや
多くなり、Ｃ型は対照区と同程度のカルシウム含有量を
示した。顆粒状区のＫＷＥにおいてＢ型、Ｃ型は対照区
よりもカルシウム含有量が多くなり、Ａ型は混合区のＫ
ＷＥと同程度であった。顆粒状区のでんぷんにおいて、
Ａ型、Ｂ型、Ｃ型いずれも対照区とほぼ同程度のカルシ
ウム含有量となり、デキストリンでは、Ａ型、Ｂ型、Ｃ
型いずれも対照区よりもカルシウム含有量は多くなっ
た。In the granular group, the lignin type A and the lignin type B had slightly higher calcium contents than the control group, and the type C showed the same calcium content as the control group. In KWE of granular section, B type and C type have higher calcium content than control section, and A type has K content of mixed section.
It was comparable to WE. In the starch of the granular section,
Type A, Type B and Type C all have almost the same calcium content as the control group.
Each type had a higher calcium content than the control group.
【００５１】したがって、この実施例のキノコ栽培用栄
養添加剤を添加したことによる子実体中のカルシウム含
有量に及ぼす影響は、バインダーの種類と粒径によって
異なる傾向を示すが、ＫＷＥ・Ａ型を除いたすべての顆
粒状区で、貝化石粉末のみを添加したものと同等あるい
はそれ以上のカルシウム含有量を示した。また、粒径別
による傾向は、Ｂ型がＡ型、Ｃ型に比べて子実体中のカ
ルシウム含有量が高くなる傾向を示した。Therefore, the effect of the addition of the nutrient additive for mushroom cultivation of this example on the calcium content in the fruiting body tends to vary depending on the type and particle size of the binder. Except for all the granular sections except the above, the calcium content was equal to or higher than that obtained by adding only the shell fossil powder. In addition, the tendency by particle size showed that the calcium content in the fruit body was higher in B-type than in A-type and C-type.
【００５２】[0052]
【発明の効果】この発明のキノコの栽培用培地とキノコ
の栽培方法ならびにキノコ栽培用栄養添加剤は、バイン
ダーとしてデキストリン、でんぷん、ＫＷＥ等の水溶性
多糖類やリグニンスルホン酸塩等を用いて直径１〜５ｍ
ｍの顆粒状に成形することにより、培地攪拌時の貝化石
粉末の取り扱いが簡便になり、身体に付着することもな
くなく、安全であり、収量やカルシウム含有量も増加さ
せることができるものである。The mushroom cultivation medium, the mushroom cultivation method and the mushroom cultivation nutritional additive of the present invention are prepared by using a water-soluble polysaccharide such as dextrin, starch, KWE or a lignin sulfonate as a binder. 1-5m
By molding into granules of m, the handling of shell fossil powder during stirring of the culture medium is simple, without sticking to the body, it is safe, and the yield and calcium content can be increased. is there.
【００５３】さらに、貝化石粉末を顆粒状にすることに
よっても、貝化石粉末を単独で添加する以上に子実体収
量が増加し、効率よい生産を行うことができ、カルシウ
ム含有量も増加させることができる。また、貝化石粉末
と上記バインダーとの混合によっても収量の増加が可能
となる。Further, by making the shell fossil powder into granules, the fruiting body yield can be increased more than the shell fossil powder alone is added, efficient production can be performed, and the calcium content can be increased. Can be. The yield can also be increased by mixing the shell fossil powder with the binder.
フロントページの続き (56)参考文献 特開 平７−194245（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) A01G 1/04 Continuation of the front page (56) References JP-A-7-194245 (JP, A) (58) Fields investigated (Int. Cl. 7 , DB name) A01G 1/04
Claims (6)
状に成形し、この貝化石粉末顆粒を、培地重量の０．３
〜３％添加したことを特徴とするキノコの栽培用培地。1. A fossil shell powder is formed into granules having a diameter of about 1 to 5 mm.
A culture medium for cultivating mushrooms, wherein the medium is added to the culture medium in an amount of up to 3%.
含む物質またはリグニン類とを重量比にして８〜９．
５：２〜０．５の割合で混合し、それら混合物を直径約
１〜５ｍｍの顆粒状に成形し、この貝化石粉末顆粒を培
地重量の０．３〜３％添加したことを特徴とするキノコ
の栽培用培地。2. The weight ratio of the shell fossil powder to the water-soluble polysaccharide or a substance or lignin containing the same is from 8 to 9.
5: 2 to 0.5, the mixture was formed into granules having a diameter of about 1 to 5 mm, and the shell fossil powder granules were added in an amount of 0.3 to 3% of the weight of the medium. Culture medium for growing mushrooms.
質は、でんぷん、デキストリン、カラマツ水抽出物、コ
ンニャク粉、小麦粉、糖蜜または廃糖蜜であることを特
徴とする請求項２記載のキノコの栽培用培地。3. The cultivation of mushrooms according to claim 2, wherein the water-soluble polysaccharide or the substance containing the same is starch, dextrin, larch water extract, konjac flour, flour, molasses or molasses. Medium.
状に成形し、この貝化石粉末顆粒を培地重量の０．３〜
３％添加してキノコの栽培用培地を調整し、この栽培用
培地に種菌を接種して適温適湿で培養し、子実体を発生
させ成長させることを特徴とするキノコの栽培方法。4. The shell fossil powder is formed into granules having a diameter of about 1 to 5 mm, and the shell fossil powder granules are added to a medium having a weight of 0.3 to 5 mm.
A method for cultivating a mushroom, comprising adding 3% to a culture medium for mushroom cultivation, inoculating the cultivation medium with a seed fungus, culturing at an appropriate temperature and humidity, and generating and growing fruit bodies.
含む物質またはリグニン類とを重量比にして８〜９．
５：２〜０．５の割合で混合し、それら混合物を直径約
１〜５ｍｍの顆粒状に成形し、この貝化石粉末顆粒を培
地重量の０．３〜３％添加してキノコの栽培用培地を調
整し、この栽培用培地に種菌を接種して適温適湿で培養
し、子実体を発生させ成長させることを特徴とするキノ
コの栽培方法。5. A weight ratio of shell fossil powder to a water-soluble polysaccharide, a substance containing the same, or a lignin, from 8 to 9.
5: 2 to 0.5 at a ratio, the mixture is formed into granules having a diameter of about 1 to 5 mm, and the shell fossil powder granules are added at 0.3 to 3% of the weight of the medium to grow mushrooms. A method for cultivating a mushroom, which comprises adjusting a medium, inoculating a seed medium into the medium for cultivation, culturing the medium at an appropriate temperature and humidity, and generating and growing fruit bodies.
含む物質またはリグニン類とを重量比にして８〜９．
５：２〜０．５の割合で混合し、それら混合物を直径約
１〜５ｍｍの顆粒状に成形して成ることを特徴とするキ
ノコ栽培用栄養添加剤。6. A fossil shellfish powder and a water-soluble polysaccharide, a substance containing the same or lignin in a weight ratio of 8 to 9.
5: A nutrient additive for cultivating mushrooms, comprising mixing the mixture at a ratio of 2 to 0.5, and forming the mixture into granules having a diameter of about 1 to 5 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP36508799A JP3263689B2 (en) | 1999-12-22 | 1999-12-22 | Mushroom cultivation medium, mushroom cultivation method and nutrient additive for mushroom cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP36508799A JP3263689B2 (en) | 1999-12-22 | 1999-12-22 | Mushroom cultivation medium, mushroom cultivation method and nutrient additive for mushroom cultivation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001178264A JP2001178264A (en) | 2001-07-03 |
| JP3263689B2 true JP3263689B2 (en) | 2002-03-04 |
Family
ID=18483397
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP36508799A Expired - Fee Related JP3263689B2 (en) | 1999-12-22 | 1999-12-22 | Mushroom cultivation medium, mushroom cultivation method and nutrient additive for mushroom cultivation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3263689B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6221039B2 (en) * | 2014-02-28 | 2017-11-01 | 岡山県 | Additive for matsutake mycelium medium and method for culturing matsutake mycelium |
| CN104016782B (en) * | 2014-05-07 | 2016-02-10 | 合肥福泉现代农业科技有限公司 | A kind of culture medium for golden mushroom containing gac |
| CN103980048A (en) * | 2014-05-22 | 2014-08-13 | 徐州绿维现代农业科技有限公司 | Preparation raw material and cultivation method of flammulina velutipes |
| CN113913362B (en) * | 2021-10-18 | 2023-11-17 | 大连工业大学 | Sorbus pohuashanensis stem cell for improving anthocyanin content, culture medium and culture method |
-
1999
- 1999-12-22 JP JP36508799A patent/JP3263689B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001178264A (en) | 2001-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100782438B1 (en) | New Cultivated Mushrooms | |
| CN110934053A (en) | Formula and processing method of flower and horticultural cultivation medium | |
| CN102498937A (en) | Method for culturing oyster mushroom | |
| JPH09191764A (en) | Cultivation of edible fungus capable of enriching nutrient of herbaceous plant suitable for cultivating edible fungus | |
| CN106748444B (en) | Formula of high-yield covering soil material for agaricus bisporus and preparation and cultivation method thereof | |
| CN104945151B (en) | A kind of epicormic branch cuttage matrix and its preparation method for great bilberry | |
| JP3263689B2 (en) | Mushroom cultivation medium, mushroom cultivation method and nutrient additive for mushroom cultivation | |
| JP2610660B2 (en) | Culture medium for mushroom cultivation | |
| CN104926511A (en) | Application of post-sprout-harvesting seed husks/embryoid bodies and rhizome substrate in preparation of solid spawn of edible fungi | |
| JP3823285B2 (en) | Artificial medium for growing eringi and method for cultivating eringi using the artificial medium | |
| Isa et al. | Physical and chemical properties of different media mixture as a growing medium for containerized plant | |
| Kaur et al. | Paddy straw and maize stalks compost for cultivation of agaricus bisporus | |
| JP2013070635A (en) | Culture medium for culturing mushroom | |
| CN109042073A (en) | A kind of edible mushroom dedicated biomass particle and preparation method thereof | |
| CN102731189B (en) | Water dropwort top dressing formula and top dressing method | |
| JP2673001B2 (en) | Culture medium for mushroom cultivation | |
| CN109169173A (en) | A kind of Schisandra chinensis cutting medium and the breeding method of Schisandra chinensis | |
| JP3798887B2 (en) | Mushroom cultivation medium containing cotton linter and method of use thereof | |
| CN106962055A (en) | A kind of method that raising seedling of cotton nutritive cube is prepared with maize straw | |
| CN112997842B (en) | General seedling substrate based on straw and wormcast fermentation product and preparation method | |
| CN107459382A (en) | A kind of champignon compost of straw substrate and preparation method thereof | |
| CN117898168A (en) | Mushroom stick conditioner for industrial cultivation of pleurotus geesteranus, and preparation method and application thereof | |
| SU1122266A1 (en) | Nutrient medium for growing seed micelium of summer honey agaric | |
| JP2007075093A (en) | Soil dressing using beer cake, shochu lees, etc. and method for producing the same | |
| CN115349399A (en) | Edible fungus culture medium and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313117 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081221 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081221 Year of fee payment: 7 |
|
| R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
| R371 | Transfer withdrawn |
Free format text: JAPANESE INTERMEDIATE CODE: R371 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081221 Year of fee payment: 7 |
|
| R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313117 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081221 Year of fee payment: 7 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| LAPS | Cancellation because of no payment of annual fees |