JP3259800B2 - Feed for aquaculture - Google Patents
Feed for aquacultureInfo
- Publication number
- JP3259800B2 JP3259800B2 JP02426294A JP2426294A JP3259800B2 JP 3259800 B2 JP3259800 B2 JP 3259800B2 JP 02426294 A JP02426294 A JP 02426294A JP 2426294 A JP2426294 A JP 2426294A JP 3259800 B2 JP3259800 B2 JP 3259800B2
- Authority
- JP
- Japan
- Prior art keywords
- feed
- larvae
- fish
- protein
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Description
【0001】[0001]
【産業上の利用分野】本発明は養殖ひらめ用飼料に関す
る。更に、詳しくは、ひらめ稚仔魚の生育を促進し、生
残率を高めることができる養殖ひらめ用飼料に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a feed for aquaculture. More specifically, the present invention relates to a feed for cultured flounder, which can promote the growth of larvae of larvae and increase the survival rate.
【0002】[0002]
【従来の技術】海産魚類の種苗生産は近年の技術的な発
展により、マダイ、クロダイ、ヒラメなどをはじめとし
て大量培養が可能になった。しかしそれらの生産規模は
サケ、マス類と比較すると両者の間には大きい差があり
サケ、マスに比べてその規模は百分の一にも達していな
い。2. Description of the Related Art In recent years, technical development of marine fish seed and seedling production has enabled large-scale cultivation of red sea bream, black porgy, flounder and the like. However, the scale of their production differs greatly from salmon and trout, and their scale is less than one-hundredth compared to salmon and trout.
【0003】この両者の大きい差は養殖用種苗生産の歴
史の差によるところも大であるが、養殖技術上からは初
期飼料の供給の差異によるところが大きい。即ちサケ、
マス類の初期飼料が配合飼料であるのに対してマダイ、
クロダイ、ヒラメ等では初期飼料として生物餌料が不可
欠であるところにその差異が発生する源と考えられる。The large difference between the two is largely due to the difference in the history of the production of seedlings for aquaculture, but from the viewpoint of aquaculture technology, it is largely due to the difference in the supply of the initial feed. That is, salmon,
Red trout, whereas the initial feed for trout is a compound feed
In the case of black porgy, flounder, etc., it is considered that the difference occurs where biological feed is indispensable as the initial feed.
【0004】生物餌料の確保には多大の設備、維持費、
労力が要求され、そのため稚仔魚の種苗生産数が限定さ
れている。この対策として生物餌料にかわる微粒子飼料
の研究開発が推進されている。A large amount of equipment, maintenance costs,
Effort is required, which limits the production of juveniles and larvae. As a countermeasure, research and development of fine particle feeds instead of biological feeds are being promoted.
【0005】微粒子飼料が備えるべき要件として、その
サイズ、比重、及び沈降速度の調節、栄養素の溶出防止
等に加えて本質的な要件として必要な栄養素を十分に含
有すること、摂餌後、消化管で栄養素が消化吸収される
ことが必要とされる。[0005] In addition to controlling the size, specific gravity, and sedimentation rate, preventing the elution of nutrients, etc., the fine particle feed should have sufficient nutrients as essential requirements, after feeding, digestion after feeding. It is required that nutrients are digested and absorbed in the tubes.
【0006】微粒子飼料の栄養効果について稚仔魚の消
化吸収機構の研究では糖質脂質の消化吸収は成魚と同様
に行われるのに対してたん白質の消化吸収様式は成魚と
全く異なることが知られている。稚仔魚のたん白質消化
吸収能力に合ったたん白質として水溶性たん白質が必要
とされ、また人為的に消化吸収能力を補う目的として消
化酵素の飼料への添加も試みられている。Studies on the nutritional effect of fine particle feed indicate that digestion and absorption of carbohydrate lipids are performed in the same manner as adult fish, whereas digestion and absorption of protein is completely different from that of adult fish. ing. A water-soluble protein is required as a protein suitable for the protein digestion and absorption capacity of larvae, and the addition of digestive enzymes to feed has been attempted to artificially supplement the digestion and absorption capacity.
【0007】以上のように、魚類の中でもひらめは養殖
が特に困難であり、従来からその飼料に工夫が試みられ
てきた。しかし、たん白質が主流であり、本発明の植物
性ペプチドを利用した飼料は知られていない。[0007] As described above, it is particularly difficult to cultivate inoculated fish among fish, and contrivances have been made on feeds thereof. However, protein is the mainstream, and no feed utilizing the plant peptide of the present invention is known.
【0008】一方、魚類用飼料として、特公昭55ー2
7784号公報には、動物の結合組織の分解生成物であ
るオリゴペプチドまたはポリペプチドを摂餌誘引物質と
して魚類用餌料に含有させる試みが行われている。これ
は、同公報に記載するように、魚類は飼料に対する嗜好
性が強く、植物性たん白では摂取行動を起こさない点を
改善したものである。On the other hand, as a feed for fish, Japanese Patent Publication No. 55-2
No. 7,784, an attempt is made to incorporate oligopeptides or polypeptides, which are degradation products of connective tissues of animals, into fish foods as food attractants. This is an improvement over fish, as described in the publication, in that fish have a strong preference for feed and do not take ingestion behavior with vegetable proteins.
【0009】本発明は、摂餌誘引物を用いなくても、植
物性たん白源でありながら自体摂餌され、ひらめ稚仔魚
の生育を促進し、生残率を高めることができる養殖ひら
め用飼料である点で目的、構成及び効果において特公昭
55ー27784号公報記載の発明とは異なるものであ
る。[0009] The present invention relates to a culture breeding halo that can be used as a vegetable protein source by itself, without the use of a feed attractant, to promote the growth of the larvae and to increase the survival rate. It differs from the invention described in Japanese Patent Publication No. 55-27784 in terms of purpose, constitution and effect in that it is a feed.
【0010】[0010]
【発明が解決しようとする課題】本発明者等は、養殖が
困難とされてきたひらめの養殖用飼料、詳しくは、ひら
め稚仔魚の生育を促進し、生残率を高めることができる
養殖ひらめ用飼料を目的とした。DISCLOSURE OF THE INVENTION The present inventors have developed a feed for cultivation of Japanese radish, which has been considered difficult to cultivate, and more specifically, a cultured feed which promotes the growth of larvae and larvae of larvae and can increase the survival rate. It was intended for feed.
【0011】[0011]
【課題を解決するための手段】本発明者等はこのような
目的を達成するべく鋭意検討の結果、特公昭55ー27
784号公報において摂餌行動を起こさないと記載され
ている植物性たん白に注目し、これを特定の分子量まで
加水分解した植物たん白加水分解物が、摂餌効果に優れ
るのみならず、目的とするひらめ稚仔魚の生育を促進
し、生残率を高めることができる知見を得て本発明を完
成するに到った。Means for Solving the Problems The present inventors have made intensive studies in order to achieve such an object, and as a result, have found a result of Japanese Patent Publication No. 55-27.
No. 784 describes a vegetable protein which is described as not causing a feeding behavior, and a plant protein hydrolyzate obtained by hydrolyzing it to a specific molecular weight not only has an excellent feeding effect, but also has a purpose. The present invention has been completed by obtaining the knowledge that can promote the growth and increase the survival rate of the larvae of the larvae.
【0012】即ち、本発明は、飼料100重量部中植物
たん白加水分解物を1〜30重量部含有することを特徴
とする養殖ひらめ用飼料である。That is, the present invention is a feed for aquaculture inoculation, wherein 1 to 30 parts by weight of a plant protein hydrolyzate is contained in 100 parts by weight of the feed.
【0013】本発明の植物たん白加水分解物は、平均分
子量は200〜10,000(好ましくは300〜5,
000)のものが適当である。分子量が大きいものでは
ひらめ稚仔魚の生育促進効果や生存率を高める効果が劣
り、分子量が小さくなりアミノ酸までなると飼料の浸透
圧が上昇したり、溶解しやすくなる等して飼料として適
当でなくなる。The plant protein hydrolyzate of the present invention has an average molecular weight of 200 to 10,000 (preferably 300 to 5,
000) is suitable. If the molecular weight is large, the effect of promoting the growth and survival of the larvae of the larvae is inferior. If the molecular weight is reduced to amino acids, the osmotic pressure of the feed increases, and the feed is easily dissolved, and thus it is not suitable as a feed.
【0014】かかる、植物たん白加水分解物の製造方法
の一例を示す。即ち、本発明に用いる植物たん白加水分
解物は、植物性たん白を酵素を用いて加水分解して得る
ことが出来る。An example of a method for producing such a plant protein hydrolyzate will be described. That is, the plant protein hydrolyzate used in the present invention can be obtained by hydrolyzing a vegetable protein using an enzyme.
【0015】このとき。原料となる植物性たん白は大
豆、菜種、落花生等の油糧種子たん白を用いることがで
きるが好ましくは入手の比較的容易でたん白含有率の高
い大豆たん白が適当である。At this time. The vegetable protein used as a raw material may be an oil seed protein such as soybean, rapeseed or peanut, but soybean protein which is relatively easily available and has a high protein content is suitable.
【0016】又、加水分解に用いる酵素としては食品の
安全性の見地より食品に使用しうるたん白分解酵素が適
当であり、その例としてはアスペルギルス属、リゾープ
ス属等の微生物が産生するたん白分解酵素等を用いるこ
とが出来る。As the enzyme used for the hydrolysis, a protein-degrading enzyme that can be used in foods is suitable from the viewpoint of food safety, and examples thereof include proteins produced by microorganisms such as Aspergillus and Rhizopus. Decomposing enzymes and the like can be used.
【0017】酵素による加水分解は常法により行うが、
その分解率は15%TCA(トリクロル酢酸)可溶率7
0%以上(好ましくは90%以上)であるものが適当で
ある。このように分解された植物たん白加水分解物はそ
のまま用いることも出来るが、必要により不溶性画分を
除いて用いることも出来る。The hydrolysis with an enzyme is carried out by a conventional method.
Its decomposition rate is 15% TCA (trichloroacetic acid) solubility 7
Those having 0% or more (preferably 90% or more) are suitable. The hydrolyzate of the plant protein thus decomposed can be used as it is, but if necessary, the insoluble fraction can be removed and used.
【0018】このようにして得られた植物たん白加水分
解物の平均分子量は、前述のように、200〜10,0
00(好ましくは300〜5,000)のものが適当で
ある。The average molecular weight of the plant protein hydrolyzate thus obtained is, as described above, 200 to 10,000.
00 (preferably 300 to 5,000) is suitable.
【0019】本発明の飼料は、組成としてたん白成分を
40〜70重量%、好ましくは約50〜60重量%を含
有するものが適当である。The feed of the present invention suitably contains 40 to 70% by weight, preferably about 50 to 60% by weight of a protein component as a composition.
【0020】本発明の飼料は、前述の植物たん白加水分
解物を1重量%〜30重量%、好ましくは3〜25重量
%含むことが適当である。通常、本発明の飼料中のたん
白成分の内の3重量重量%以上、好ましくは10〜80
重量%を前述の植物たん白加水分解物で置換したものが
適当である。置換割合が多くなると該飼料を顆粒化する
ことが困難となる。The feed of the present invention suitably contains the above-mentioned plant protein hydrolyzate in an amount of 1 to 30% by weight, preferably 3 to 25% by weight. Usually, 3% by weight or more, preferably 10 to 80% by weight of the protein component in the feed of the present invention.
It is suitable that the weight% is replaced with the above-mentioned vegetable protein hydrolyzate. When the replacement ratio increases, it becomes difficult to granulate the feed.
【0021】本発明の飼料中前述の植物たん白加水分解
物が少ないとひらめ稚仔魚の成長率、生存率を高める効
果が少なく、多過ぎると生育阻害を起こすので好ましく
ない。これは、マダイ、ひらめ等養殖種苗生産が困難な
魚に共通するものであり、他の魚の養殖とは異なるもの
である。If the amount of the above-mentioned plant protein hydrolyzate in the feed of the present invention is low, the effect of increasing the growth rate and survival rate of the larvae of young larvae is small. This is common to fish such as red sea bream and inoculated fish, for which production of cultured seeds is difficult, and is different from the cultivation of other fish.
【0022】尚、植物たん白加水分解物以外のたん白質
成分としてはオキアミ、魚類ミール類、鶏卵加工品、牛
乳加工品、ゼラチン、魚粉、魚介類エキス、酵母エキ
ス、魚卵エキスを併用することができる。As protein components other than the plant protein hydrolyzate, krill, fish meal, processed egg, processed milk, gelatin, fish meal, seafood extract, yeast extract, fish egg extract may be used in combination. Can be.
【0023】本発明の飼料には、前述の植物たん白加水
分解物、その他のたん白質の他、炭水化物、脂肪、ビタ
ミン、ミネラル、n−3高度不飽和脂肪酸および大豆レ
シチン等の飼料性リン脂質を含有することができる。The feed of the present invention includes, in addition to the above-mentioned vegetable protein hydrolyzate and other proteins, feed-based phospholipids such as carbohydrates, fats, vitamins, minerals, n-3 polyunsaturated fatty acids and soybean lecithin. Can be contained.
【0024】n−3高度不飽和脂肪酸はひらめのような
海水魚に必須の脂肪酸であり、大豆レシチン等の飼料性
リン脂質は稚仔魚の養殖に必要な成分であるので本発明
の飼料に含むことが出来る。The n-3 polyunsaturated fatty acid is an essential fatty acid for seawater fish such as the rainbow trout, and the feed phospholipid such as soybean lecithin is a component necessary for the cultivation of larvae and is included in the feed of the present invention. I can do it.
【0025】本発明の飼料の形態はひらめの摂取しやす
い粒経、浮遊性、沈降速度を持ちしかも水中で栄養素が
溶出せず、消化管で消化吸収されるようにマイクロカプ
セル化等により微粒子飼料とすることができる。The form of the feed of the present invention has a particle size, floatability and sedimentation rate which are easy to ingest for the inflammation, and the micronutrients are microencapsulated so that nutrients are not eluted in water and digested and absorbed in the digestive tract. It can be.
【0026】本発明の飼料は稚仔魚の日齢によりひらめ
稚仔魚期に生物餌料と併用もしくは単独で適量を30分
〜1時間間隔で給餌することが出来る。[0026] The feed of the present invention can be fed together with a biological feed or in an appropriate amount alone at intervals of 30 minutes to 1 hour at the stage of the larvae and larvae depending on the age of the larvae.
【0027】[0027]
【実施例】以下、実施例により本発明の実施態様を説明
する。The embodiments of the present invention will be described below with reference to examples.
【0028】実施例1 分離大豆蛋白(不二製油(株)製「フジプロ−R」)1
00重量部(以下、部)をpH7の5%水溶液となし、
プロチンFN(大和化成(株)製:アスペルギルス属起
源)1部を用い、50℃で5時間酵素分解した後、70
℃で30分間加熱して酵素を失活させ、冷却後遠心分離
して得た上澄みを噴霧乾燥して大豆たん白加水分解物を
製造した。尚、平均分子量は676であった。Example 1 Isolated soybean protein (“Fujipro-R” manufactured by Fuji Oil Co., Ltd.) 1
00 parts by weight (hereinafter referred to as “parts”) as a 5% aqueous solution of pH 7,
After enzymatic degradation at 50 ° C. for 5 hours using 1 part of Protin FN (manufactured by Daiwa Kasei Co., Ltd .: origin of Aspergillus),
The mixture was heated at 30 ° C. for 30 minutes to inactivate the enzyme. After cooling, the supernatant obtained by centrifugation was spray-dried to produce a soybean protein hydrolyzate. Incidentally, the average molecular weight was 676.
【0029】次に、38日齢ひらめ稚仔魚を予備飼育水
槽より300尾宛100L実験水槽に移したものを5槽
準備し、実験水槽とした。実験期間中海水の温度は17
゜Cに保持した。Next, five 38-day-old larvae and larvae were transferred from the preliminary breeding aquarium to a 300-L 100-L experimental aquarium to prepare an experimental aquarium. The temperature of the seawater during the experiment was 17
It was kept at ゜ C.
【0030】実験飼料として前記大豆たん白加水分解物
を表1のひらめ養殖飼料に0、2.5、5.0、10.
0、20.0重量部をカゼインと表1に示すように置換
して添加、常法により微粒子飼料とした。表1に実験飼
料組成を示す。単位は重量部である。5群はコントロー
ルである。As a test feed, the soybean protein hydrolyzate was added to the inoculated culture feed shown in Table 1 at 0, 2.5, 5.0, 10.
0 and 20.0 parts by weight were replaced with casein as shown in Table 1 and added to obtain a fine particle feed by a conventional method. Table 1 shows the experimental feed composition. The unit is parts by weight. Group 5 is a control.
【0031】[0031]
【表1】 ------------------------------------------------------------- 成分 1群 2群 3群 4群 5群 ------------------------------------------------------------- 白身魚ミール 30.0 30.0 30.0 30.0 30.0 カゼイン 11.3 20.6 25.3 27.7 30.0 大豆たん白加水分解物 20.0 10.0 5.0 2.5 0 デキストリン 7.0 7.0 7.0 7.0 7.0 ビタミン混合物 5.3 5.3 5.3 5.3 5.3 ミネラル混合物 5.0 5.0 5.0 5.0 5.0 すけとう鱈肝油 8.0 8.0 8.0 8.0 8.0 n−3高度不飽和脂肪酸 0.5 0.5 0.5 0.5 0.5 大豆レシチン 4.0 4.0 4.0 4.0 4.0 セルローズ 0.9 1.6 1.9 2.0 2.2 ゼイン 8.0 8.0 8.0 8.0 8.0 ------------------------------------------------------------- 計 100 100 100 100 100 -------------------------------------------------------------[Table 1] ---------------------------------------------- --------------- Ingredients 1 group 2 group 3 group 4 group 5 group ------------------------ ------------------------------------- White fish meal 30.0 30.0 30.0 30.0 30.0 casein 11.3 20.6 25.3 27.7 30.0 Soy protein hydrolyzate 20.0 10.0 5.0 2.5 0 Dextrin 7.0 7.0 7.0 7.0 7.0 Vitamin mixture 5.3 5.3 5.3 5.3 5.3 Mineral mixture 5.0 5.0 5.0 5.0 5.0 Pork cod liver oil 8.0 8.0 8.0 8.0 8.0 n-3 highly unsaturated fatty acids 0.5 0.5 0.5 0.5 0.5 Soy lecithin 4.0 4.0 4.0 4.0 4.0 Cellulose 0.9 1.6 1.9 2.0 2.2 Zein 8.0 8.0 8.0 8.0 8.0 ---------------------------- --------------------------------- Total 100 100 100 100 100 ----------- --------------------------------------------------
【0032】39日齢より実験を開始した。給餌は午前
9時より1時間毎に16時まで8回、17時には生物餌
料としてアルテミアを与え、1日9回給餌した。飼料量
は日齢がたつにつれて養殖飼料0.31g〜0.50g/
回/魚、アルテミアは55〜80匹/回/魚とした。The experiment was started at the age of 39 days. Feeding was carried out eight times from 9:00 am to 16:00 every hour, and at 17:00 Artemia was given as a biological feed and fed nine times a day. The amount of feed is from 0.31 g to 0.50 g / aqua feed as the age increases.
The number of times / fish and artemia was 55 to 80 / time / fish.
【0033】実験開始後14日、52日齢時の稚仔魚約
50尾の体長、生残尾数、色素異常率を測定した結果は
表2の通りである。Table 2 shows the results of measurement of the body length, the number of surviving tails, and the pigment abnormality rate of about 50 larvae at the age of 52 days, 14 days after the start of the experiment.
【0034】[0034]
【表2】52日齢測定結果 ----------------------------------------------------- 群 N 体長平均(mm) 標準偏差 生残数 ----------------------------------------------------- 1 52 17.91 1.877 194 2 49 18.18 2.112 215 3 49 17.43 1.888 200 4 54 17.31 1.722 195 5 53 17.01 1.833 194 -----------------------------------------------------[Table 2] 52 days of age measurement results ----------------------------------------- ------------ Group N Average length (mm) Standard deviation Survival number -------------------------- --------------------------- 1 52 17.91 1.877 194 2 49 18.18 2.112 215 3 49 17.43 1.888 200 4 54 17.31 1.722 195 5 53 17.01 1.833 194 ------------------------------------------------ -----
【0035】この結果、コントロールの5群に比べて1
〜4群はいずれも生育が促進され、とくに1、2群では
有意差が認められた。生残数においても5群に比べて1
〜4群では良い傾向が見られた。又、色素異常率は各群
に差なく、大豆たん白加水分解物を与えた群においても
異常は認められなかった。As a result, compared to the five control groups, 1 was obtained.
Growth was promoted in all of the groups No. 1 to No. 4, and a significant difference was recognized particularly in the groups Nos. 1 and 2. Survival number is 1 compared to 5 groups
A good tendency was observed in groups 4 to 4. The pigment abnormality rate did not differ between the groups, and no abnormality was observed in the group to which the soybean protein hydrolyzate was given.
【0036】[0036]
【発明の効果】本発明のひらめ用飼料を給餌することに
より、ひらめ稚子魚の生育を促進し、生残率を高めるこ
とができ、ひらめ養殖の生産性の向上、飼料効率の向上
を図ることが出来、ひらめ養殖家にとって益するところ
が大きくなり、産業の発達に寄与するものである。EFFECT OF THE INVENTION By feeding the invented feed for the present invention, it is possible to promote the growth of the Japanese larvae and the survival rate of the larvae, and to improve the productivity and the feed efficiency of the aquaculture. The benefits and benefits for the incubator will increase and contribute to the development of the industry.
Claims (2)
を1〜30重量部含有することを特徴とするひらめ稚仔
魚養殖用飼料。The present invention relates to a fry larva which contains 1 to 30 parts by weight of a plant protein hydrolyzate in 100 parts by weight of a feed.
Feed for fish farming .
〜10,000の植物たん白加水分解物である請求項1
のひらめ稚仔魚養殖用飼料。2. A plant protein hydrolyzate having an average molecular weight of 200
2. A plant protein hydrolyzate of up to 10,000.
Noirame fry and larvae feed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP02426294A JP3259800B2 (en) | 1994-02-22 | 1994-02-22 | Feed for aquaculture |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP02426294A JP3259800B2 (en) | 1994-02-22 | 1994-02-22 | Feed for aquaculture |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH07227223A JPH07227223A (en) | 1995-08-29 |
JP3259800B2 true JP3259800B2 (en) | 2002-02-25 |
Family
ID=12133328
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP02426294A Expired - Fee Related JP3259800B2 (en) | 1994-02-22 | 1994-02-22 | Feed for aquaculture |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP3259800B2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100379354C (en) * | 2002-07-24 | 2008-04-09 | 不二制油株式会社 | Feed for fry young fishes and method of producing hydrolyzate of low-phytin vegetable protein to be used therein |
JP5300328B2 (en) * | 2008-05-30 | 2013-09-25 | 不二製油株式会社 | Fish feed for green liver disease prevention |
-
1994
- 1994-02-22 JP JP02426294A patent/JP3259800B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JPH07227223A (en) | 1995-08-29 |
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