JP3182076B2 - Gel-like immobilized cells - Google Patents
Gel-like immobilized cellsInfo
- Publication number
- JP3182076B2 JP3182076B2 JP7819296A JP7819296A JP3182076B2 JP 3182076 B2 JP3182076 B2 JP 3182076B2 JP 7819296 A JP7819296 A JP 7819296A JP 7819296 A JP7819296 A JP 7819296A JP 3182076 B2 JP3182076 B2 JP 3182076B2
- Authority
- JP
- Japan
- Prior art keywords
- gel
- cells
- immobilized
- particles
- fibers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、海，河川，湖沼，
池などの水質保全に有用なゲル状固定化菌体に関する。TECHNICAL FIELD The present invention relates to a sea, a river, a lake, a marsh,
The present invention relates to a gel-like immobilized microbial cell useful for maintaining water quality of a pond.
【０００２】本発明に係るゲル状固定化菌体は、海，河
川，湖沼，池などに投入又は設置して該水域の水質保全
に使用される。[0002] The immobilized gel-like cells according to the present invention are introduced or installed in the sea, rivers, lakes, marshes, ponds, and the like, and are used for preserving the water quality of the water area.
【０００３】[0003]
【従来の技術】周知の通り、ロドシュードモナス・カプ
シュラータ（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ ｃａ
ｐｓｕｌａｔａ：微工研菌寄第８７９号），ロドシュー
ドモナス・スフェロイデス（Ｒｈｏｄｏｐｓｅｕｄｏｍ
ｏｎａｓ ｓｐｈｅｒｏｉｄｅｓ：ＩＦＯ第１２２０３
号），ロドスピリラム・ルブラム（Ｒｈｏｄｏｓｐｉｒ
ｉｌｌｕｍ ｒｕｂｒｕｍ：微工研菌寄第８７８号）及
びクロマチウム・ビノサム（Ｃｈｒｏｍａｔｉｕｍ ｖ
ｉｎｏｓｕｍ：微工研菌寄第８９０号）−以下、これ等
を一括して「特定光合成細菌」ともいう−は、肥料，飼
料，有機廃水処理剤等々の各種用途に用いられている有
用菌であり、特に、最近では、その有機物汚泥の浄化能
力に注目され、海，河川，湖沼，池などにおける魚貝類
養殖場水域の水質保全に活用されている。2. Description of the Related Art As is well known, Rhodopseudomonas ca.
psulata: Microbiological Research Laboratories No. 879), Rhodopseudomonas sphaeroides (Rhodopseudom)
onas spheroides: IFO No. 12203
No.), Rhodospirir (Rhodospir)
illum rubrum: No. 878 of Japan Institute of Microbiological Research and Chromiumium vinosum (Chromatium v)
(Inosum: Microtechnical Laboratory No. 890)-These are also collectively referred to as "specific photosynthetic bacteria"-which are useful bacteria used in various applications such as fertilizers, feed, organic wastewater treatment agents and the like. In particular, recently, attention has been paid to its ability to purify organic sludge, and it has been utilized for the conservation of water quality in fish and shellfish cultivation areas in seas, rivers, lakes, ponds, and the like.
【０００４】特定光合成細菌菌体が各種対象水域の水質
保全に用いられるに当たっては、種々様々な態様が採ら
れており、例えば、特公平７−１１５０２９号公報には
特定光合成細菌菌体と下水汚泥醗酵物とを袋に充填して
対象水域の底部に浮遊する汚泥の沈降・浄化剤として用
いる態様が示されており、特開平５−２４７３７８号公
報には特定光合成細菌菌体、担体（例えば、パーライト
や含水ゲル状担体）及び該菌体の栄養成分を含有する塗
料を調製して該塗料を対象水域の護岸用コンクリートブ
ロック等に塗布することによって対象水域の魚貝類の繁
殖と水質の浄化をはかる態様が示されている。[0004] In the use of the specific photosynthetic bacterial cells for preserving the water quality of various target water areas, various modes are adopted. For example, Japanese Patent Publication No. 7-115029 discloses a specific photosynthetic bacterial cell and sewage sludge. An embodiment in which a fermentation product is filled in a bag and used as a sedimentation / purification agent for sludge floating at the bottom of a target water area is disclosed. JP-A-5-247378 discloses a specific photosynthetic bacterial cell, a carrier (for example, Perlite or hydrated gel carrier) and a paint containing nutrients of the cells are prepared, and the paint is applied to a seawall concrete block or the like in the target water area to propagate the fish and shellfish in the target water area and purify the water quality. The measuring mode is shown.
【０００５】また、特開平１−６０３１４号公報には特
定光合成細菌菌体と該菌体用培地とを水溶性珪酸化合物
（例えば、珪酸カリウムや珪酸メチル）を用いてゲル状
物質とした固定化菌体を土壌の肥沃化、植物の育成のた
めに水田等に施用したり、排水処理のための種菌とする
態様が示されている。Japanese Patent Application Laid-Open No. 1-60314 discloses immobilization of a specific photosynthetic bacterial cell and a culture medium for the cell as a gel-like substance using a water-soluble silicate compound (eg, potassium silicate or methyl silicate). There is disclosed an embodiment in which the cells are applied to paddy fields or the like for fertilization of soil and cultivation of plants, or as seed bacteria for wastewater treatment.
【０００６】[0006]
【発明が解決しようとする課題】前掲特開平１−６０３
１４号に示されている固定化菌体は調製が容易であると
共に使用時における取扱い性も良いが、該固定化菌体
を、海，河川，湖沼，池などの水質保全用途に用いる場
合には、次のような問題がある。SUMMARY OF THE INVENTION The above-mentioned Japanese Patent Application Laid-Open No. 1-603.
The immobilized cells shown in No. 14 are easy to prepare and easy to handle at the time of use. However, when the immobilized cells are used for water quality preservation use in seas, rivers, lakes, ponds, and the like. Has the following problems.
【０００７】即ち、前記固定化菌体を対象水域に施用す
ると、動物性プランクトンや魚貝類などに捕食された
り、水流によって損傷することがあり、かかる場合に
は、施用後２〜３週間でゲル状物が崩壊してしまうとい
う問題点がある。That is, when the immobilized cells are applied to a target water area, they may be eaten by zooplankton or fish and shellfish, or may be damaged by water currents. There is a problem that the material collapses.
【０００８】本発明は、前記問題点を解消し、対象水域
において、より長期間にわたりゲル状構造及び菌体活性
が維持できる固定化菌体を提供することを技術的課題と
する。[0008] It is a technical object of the present invention to solve the above-mentioned problems and to provide immobilized cells capable of maintaining a gel-like structure and cell activity for a longer period of time in a target water area.
【０００９】[0009]
【課題を解決するための手段】前記技術的課題は次の通
りの本発明によって達成できる。即ち、本発明は、ロド
シュードモナス・カプシュラータ（同上),ロドシュード
モナス・スフェロイデス（同上），ロドスピリラム・ル
ブラム（同上）及びクロマチウム・ビノサム（同上）か
ら選ばれる一種又は二種以上の光合成細菌菌体と該菌体
の栄養基質とを、ゼラチン，海藻多糖類，ポリビニルア
ルコール及びアルギン酸ソーダから選ばれる一種又は二
種以上のゲル化材料にて固定化してなるゲル状固定化菌
体において、該ゲル状固定化菌体にクロボク土粒，オオ
ヤ石粒，炭粒，ヒル石粒及び高分子樹脂製スポンジ粒か
ら選ばれる一種又は二種以上の直径１〜５mmの微細孔を
もつ多孔性粒状物を含有させたことを特徴とする水質保
全用ゲル状固定化菌体である。The above technical objects can be achieved by the present invention as described below. That is, the present invention relates to one or more photosynthetic bacterial cells selected from Rhodopseudomonas capsulata (same as above), Rhodopseudomonas spheroides (same as above), Rhodospirillum rubrum (same as above), and Chromium Vinosam (same as above), and A gel-like immobilized cell obtained by immobilizing a nutrient substrate of cells with one or two or more gelling materials selected from gelatin, seaweed polysaccharide, polyvinyl alcohol and sodium alginate. The cells contained one or more porous granules having fine pores with a diameter of 1 to 5 mm selected from black and white earth grains, oyster stones, charcoal grains, leech stones, and polymer resin sponge grains. Water quality maintenance characterized by that
It is a gel-like immobilized cell for whole use .
【００１０】また、本発明は、ロドシュードモナス・カ
プシュラータ（同上),ロドシュードモナス・スフェロイ
デス（同上）及びクロマチウム・ビノサム（同上）から
選ばれる一種又は二種以上の光合成細菌菌体と該菌体の
栄養基質とを、ゼラチン，海藻多糖類，ポリビニルアル
コール及びアルギン酸ソーダから選ばれる一種又は二種
以上のゲル化材料にて固定化してなるゲル状固定化菌体
において、該ゲル状固定化菌体を天然繊維，合成繊維及
び金属繊維から選ばれる一種又は二種以上の繊維材料の
フィラー状物によって補強したことを特徴とする水質保
全用ゲル状固定化菌体である。Further, the present invention is Rhodopseudomonas-Kapushurata (ibid), Rhodopseudomonas sphaeroides (ibid)及 beauty Kuromachiumu-Binosamu Id from one or two or more of photosynthetic bacteria cells and microbial cells selected A gel-like immobilized cell obtained by immobilizing a nutrient substrate with one or two or more gelling materials selected from gelatin, seaweed polysaccharide, polyvinyl alcohol and sodium alginate. natural fibers, one selected from synthetic fibers and metal fibers or two or more fiber material
It is a gel-like immobilized cell for water quality preservation, which is reinforced with a filler-like substance .
【００１１】また、本発明は、ロドシュードモナス・カ
プシュラータ（同上),ロドシュードモナス・スフェロイ
デス（同上）及びクロマチウム・ビノサム（同上）から
選ばれる一種又は二種以上の光合成細菌菌体と該菌体の
栄養基質とを、ゼラチン，海藻多糖類，ポリビニルアル
コール及びアルギン酸ソーダから選ばれる一種又は二種
以上のゲル化材料にて固定化してなるゲル状固定化菌体
において、該ゲル状固定化菌体内にクロボク土粒，オオ
ヤ石粒，炭粒，ヒル石粒及び高分子樹脂製スポンジ粒か
ら選ばれる一種又は二種以上の直径１〜５mmの微細孔を
もつ多孔性粒状物を含有させると共に、当該固定化菌体
を天然繊維，合成繊維及び金属繊維から選ばれる一種又
は二種以上の繊維材料のフィラー状物によって補強した
ことを特徴とする水質保全用ゲル状固定化菌体である。Further, the present invention is Rhodopseudomonas-Kapushurata (ibid), Rhodopseudomonas sphaeroides (ibid)及 beauty Kuromachiumu-Binosamu Id from one or two or more of photosynthetic bacteria cells and microbial cells selected A gel-like immobilized cell obtained by immobilizing a nutrient substrate with one or more gelling materials selected from gelatin, seaweed polysaccharide, polyvinyl alcohol, and sodium alginate. One or more kinds of porous granules having fine pores with a diameter of 1 to 5 mm selected from Kuroboku soil grains, Oya stone grains, charcoal grains, leech stone grains and polymer resin sponge grains, and fixing the same. A water characterized in that the bacterium is reinforced with one or more filler materials of one or more fiber materials selected from natural fibers, synthetic fibers and metal fibers. It is a gel-like immobilized cell for quality maintenance.
【００１２】以上の通りの構成を採る本発明に用いる特
定光合成細菌菌体は、寄託機関に寄託されているものが
入手でき、また、市販されているものもあり、さらに自
然界から分離することもできる。The specific photosynthetic bacterial cells used in the present invention having the above-mentioned constitution can be obtained by depositing them with a depository institution, and some of them are commercially available, and can be further isolated from nature. it can.
【００１３】本発明に用いる特定光合成細菌菌体の栄養
基質は、前掲特開平１−６０３１４号公報にも示されて
いる通り、公知のものであり、市販品によって処方でき
る。なお、代表的な処方例を後出「実施の形態」に示し
た。The nutrient substrate of the specific photosynthetic bacterial cell used in the present invention is known as described in the above-mentioned Japanese Patent Application Laid-Open No. 1-60314, and can be formulated as a commercial product. In addition, a typical prescription example is shown in “Embodiment” below.
【００１４】本発明に用いるゲル化材料は、いずれも周
知のものであり、市販品を用いればよい。なお、海藻多
糖類としては、例えば寒天がよく知られている。The gelling materials used in the present invention are all well-known, and commercially available products may be used. As the seaweed polysaccharide, for example, agar is well known.
【００１５】本発明に用いる直径１〜５mmの微細孔をも
つ多孔性粒状物は、いずれも周知のものであり、市販品
から所要の微細孔をもつものを選定して用いればよい。
この多孔性粒状物は、特定光合成細菌菌体の担体とな
り、該菌体を強固に固定するものである。The porous granular materials having fine pores of 1 to 5 mm in diameter used in the present invention are all well-known, and those having the required fine pores may be selected from commercially available products.
The porous particulate material serves as a carrier for the specific photosynthetic bacterial cells and firmly fixes the cells.
【００１６】また、前記多孔性粒状物は、粒径２〜１０
mm程度のものを選定使用することが好ましく、この粒径
範囲のものを用いる場合には該有孔粒状物は、ゲル状物
内において骨材として働きゲル状物の程度を増大させ
る。The porous granular material has a particle size of 2 to 10.
It is preferable to select and use a particle having a diameter of about mm. When the particle having the particle size in this range is used, the perforated granular material acts as an aggregate in the gel and increases the degree of the gel.
【００１７】前記多孔性粒状物の使用量は、ゲル状物に
対して容量比で１０〜３０％が好適であり、１％未満で
は菌体固定効果とゲル状物強化効果が得られ難く、７０
％を越えるとゲル状物中の特定光合成菌体の増殖が少な
くなると共に、ゲル状物が脆くなってしまう。The amount of the porous granular material to be used is preferably 10 to 30% by volume relative to the gel material, and if less than 1%, it is difficult to obtain a cell-fixing effect and a gel-material strengthening effect. 70
%, The proliferation of specific photosynthetic bacteria in the gel-like material is reduced, and the gel-like material becomes brittle.
【００１８】本発明に用いる繊維材料には市販の各種繊
維製品が適用でき、フィラー状物，糸状物，紐状物，縄
状物及び網状物のいずれの形状の繊維製品も使用でき
る。Various types of commercially available fiber products can be applied to the fiber material used in the present invention, and any fiber product in the form of a filler, a thread, a string, a rope, and a net can be used.
【００１９】[0019]
【発明の実施の形態】本発明の代表的な実施の形態は次
の通りである。DESCRIPTION OF THE PREFERRED EMBODIMENTS A typical embodiment of the present invention is as follows.
【００２０】コハク酸１０g,リンゴ酸１０g,硫安５g,リ
ン酸一カリウム８g,硫酸マグネシウム２g,食塩１g,塩化
カルシウム０．５g 及び酵母エキス２g を水１l に溶解
した栄養基質（培地）に、ロドシュードモナス・カプシ
ュラータ（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ ｃａｐ
ｓｕｌａｔａ：微工研菌寄第８７９号）菌体とクロマチ
ウム・ビノサム（Ｃｈｒｏｍａｔｉｕｍ ｖｉｎｏｓｕ
ｍ：微工研菌寄第８９０号）菌体とを接種すると共に、
直径１〜３mmの微細孔をもつ粒径約５７mmのオオヤ石粒
１０g と直径１〜３mmの微細孔を持つ粒径約５mmのヒル
石粒１０g とを添加して、Ａ液を調製した。A nutrient substrate (medium) prepared by dissolving 10 g of succinic acid, 10 g of malic acid, 5 g of ammonium sulfate, 8 g of monopotassium phosphate, 2 g of magnesium sulfate, 1 g of sodium chloride, 0.5 g of calcium chloride and 2 g of yeast extract in 1 l of water was added. Rhodopseudomonas cap
sulata: Microtechnical Research Laboratories No. 879) Cells and Chromiumium vinosum
m: No. 890).
Liquid A was prepared by adding 10 g of Oya stone having a diameter of about 57 mm having fine pores having a diameter of 1 to 3 mm and 10 g of leech stone having a diameter of about 5 mm having fine pores having a diameter of 1 to 3 mm.
【００２１】１０％ポリビニルアルコール水溶液２l
に、前記栄養基質（培地）と同一組成の栄養基質０．８
l ，水０．７l 及び酢酸１５．２g を添加してpH１．１
のＢ液を調製した。2 l of 10% aqueous solution of polyvinyl alcohol
The nutrient substrate having the same composition as the nutrient substrate (medium) 0.8
, 0.7 l of water and 15.2 g of acetic acid.
A liquid B was prepared.
【００２２】水３l に、１０％硫酸アルミニウムカリウ
ム４００g ，炭酸水素ナトリウム４g 及びチオ硫酸ナト
リウム４g を添加してpH１１．９のＣ液を調製した。To 3 liters of water, 400 g of 10% potassium aluminum sulfate, 4 g of sodium hydrogen carbonate and 4 g of sodium thiosulfate were added to prepare a solution C having a pH of 11.9.
【００２３】２００ml容の透明容器に、Ａ液４０mlとＢ
液８０mlとＣ液８０mlとを、撹拌混合しながら同時に投
入し、さらに、セルローズ繊維製フィラー５g を添加
し、撹拌後、靜置し、光照明下・２５℃で７日間培養し
て、真紅色を呈したゲル状固定化菌体を得た。In a 200 ml transparent container, 40 ml of solution A and B
80 ml of the liquid C and 80 ml of the liquid C were simultaneously added with stirring and mixing, and 5 g of a filler made of cellulose fiber was added. After stirring, the mixture was allowed to stand, and cultured under light illumination at 25 ° C. for 7 days to obtain a crimson color. The gel-like immobilized cells exhibiting the above were obtained.
【００２４】ここに得たゲル状固定化菌体を稼働中の養
魚池の池底に設置し観察したところ、設置後３０日経過
した時点においてゲル状態を保持していることが確認で
きた。The immobilized gel-like cells obtained here were installed on the bottom of a running fishpond and observed, and it was confirmed that the gel state was maintained 30 days after the installation.
【００２５】前記の通り、ここに得たゲル状固定化菌体
は水中において長期間ゲル状態が保持できるので、使用
中に栄養基質が不足してくる場合もあり、かかる場合に
は、水中に設置したゲル状固定化菌体の近傍にパイプを
配管し該パイプによって前記栄養基質（培地）と同一組
成の栄養基質を補給することが望ましい。補給に用いる
パイプは、通常、口径２〜５mmの合成樹脂（例えば塩化
ビニル）製パイプの側面に直径１〜４mmの穴を適当間隔
置いて穿ったものが好適であり、かかるパイプをゲル状
固定化菌体表面に接近させて配管すれば、栄養基質が容
易に補給できる。As described above, the gel-like immobilized cells obtained here can maintain a gel state in water for a long period of time, so that nutrient substrates may become insufficient during use. It is preferable that a pipe be provided near the gel-like immobilized cells, and a nutrient substrate having the same composition as the nutrient substrate (medium) be supplied by the pipe. The pipe used for replenishment is usually a pipe made of synthetic resin (for example, vinyl chloride) having a diameter of 2 to 5 mm, and a hole having a diameter of 1 to 4 mm is formed at an appropriate interval on a side face thereof. By piping close to the surface of the cell, nutrient substrates can be easily supplied.
【００２６】[0026]
【実施例】以下に、実施例及び比較例を挙げる。EXAMPLES Examples and comparative examples will be described below.
【００２７】実施例１ プロピオン酸ソーダ５g,塩化アンモニウム１g,リン酸一
カリウム０．８g,塩化マグネシウム０．２g,塩化ナトリ
ウム０．１g,塩化カルシウム０．０５g,炭酸水素ナトリ
ウム０．５g 及び酵母エキス０．２g を水１l に溶解し
た栄養基質（培地）に、ロドシュードモナス・カプシュ
ラータ（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ ｃａｐｓ
ｕｌａｔａ：微工研菌寄第８７９号）菌体を接種すると
共に、直径１〜２mmの微細孔をもつ粒径約３mmのクロボ
ク土粒５g と直径１〜２mmの微細孔を持つ粒径約３mmの
活性炭粒５g とを添加して、Ａ液を調製した。Example 1 5 g of sodium propionate, 1 g of ammonium chloride, 0.8 g of monopotassium phosphate, 0.2 g of magnesium chloride, 0.1 g of sodium chloride, 0.05 g of calcium chloride, 0.5 g of sodium hydrogen carbonate and yeast extract In a nutrient substrate (medium) prepared by dissolving 0.2 g in 1 l of water, Rhodopseudomonas caps was added.
ulata: No. 879) Microbial inoculation, 5 g of Kuroboku soil granules having a diameter of about 3 mm with micropores of 1-2 mm in diameter, and a particle size of about 3 mm having micropores of 1-2 mm in diameter 5 g of activated carbon granules was added to prepare solution A.
【００２８】約４２℃に加温溶解させた３％ゼラチン水
溶液１l に、プロピオン酸ソーダ５g,塩化アンモニウム
１g,リン酸一カリウム０．８g,塩化マグネシウム０．２
g,塩化ナトリウム０．１g,塩化カルシウム０．０５g,炭
酸水素ナトリウム０．５g 及び酵母エキス０．２g を添
加し、ゆるやかに撹拌して溶解させてＢ液を調製した。In 1 liter of a 3% aqueous gelatin solution dissolved at about 42 ° C., 5 g of sodium propionate, 1 g of ammonium chloride, 0.8 g of monopotassium phosphate, 0.2 g of magnesium chloride were added.
g, 0.1 g of sodium chloride, 0.05 g of calcium chloride, 0.5 g of sodium bicarbonate and 0.2 g of yeast extract were added and gently stirred to dissolve to prepare a solution B.
【００２９】１５０ml容の透明容器に、Ａ液１０mlとＢ
液１００mlとを、撹拌混合しながら同時に投入し、撹拌
後、靜置し、室温（約２０℃）で３０分間放置してゲル
状物とし、次いで光照明下・２３℃で７日間培養して、
真紅色を呈したゲル状固定化菌体を得た。In a 150 ml transparent container, 10 ml of solution A and B
100 ml of the solution was added simultaneously with stirring and mixing. After stirring, the mixture was allowed to stand, left at room temperature (about 20 ° C.) for 30 minutes to form a gel, and then cultured under light illumination at 23 ° C. for 7 days. ,
The gel-like immobilized cells having a crimson color were obtained.
【００３０】実施例２ 前出「発明の実施の形態」において用いた栄養基質（培
地）と同一組成の栄養基質１l にアルギン酸ナトリウム
を３％濃度となるように添加し、撹拌混合した後、ロド
シュードモナス・スフェロイデス（Ｒｈｏｄｏｐｓｅｕ
ｄｏｍｏｎａｓｓｐｈｅｒｏｉｄｅｓ：ＩＦＯ第１２２
０３号）菌体とクロマチウム・ビノサム（Ｃｈｒｏｍａ
ｔｉｕｍ ｖｉｎｏｓｕｍ：微工研菌寄第８９０号）菌
体とを接種して、Ａ液を調製した。Example 2 Sodium alginate was added to 1 liter of a nutrient substrate having the same composition as that of the nutrient substrate (medium) used in the above “Embodiment of the Invention” so as to have a concentration of 3%, and the mixture was stirred and mixed. Pseudomonas spheroides
domonasspheroides: IFO No. 122
No. 03) Cell and Chromium Vinosum (Chroma)
solution (A) was prepared by inoculating the cells.
【００３１】Ａ液を口径２mmの注射筒を用いて０．１Ｍ
塩化カルシウム溶液中に滴下し、約２０℃で１０分間放
置して、該塩化カルシウム溶液中に複数個のイクラ状の
ゲルビーズを形成させた。Solution A was prepared at 0.1 M using a syringe having a diameter of 2 mm.
The solution was dropped into a calcium chloride solution and allowed to stand at about 20 ° C. for 10 minutes to form a plurality of gel-like gel beads in the calcium chloride solution.
【００３２】次いで、前記複数個のイクラ状のゲルビー
ズを光照明下・２６℃で７日間培養して真紅色を呈した
イクラ状ゲルビーズとした。Next, the plurality of salmon-like gel beads were cultured at 26 ° C. for 7 days under light illumination to obtain red salmon-like gel beads.
【００３３】次いで、容器内に直径０．１mmの金属繊維
製螺旋状ワイヤーを配置し、該容器内に前記複数個の真
紅色を呈したイクラ状のゲルビーズを充填し、室温で１
５日間放置後、該容器から該イクラ状ゲルビーズが定着
している該螺旋状ワイヤーを取り出した。ここに得たイ
クラ状ゲルビーズ形固定化菌体は、前記螺旋状ワイヤー
に強固に定着しており、水中に３０日間浸漬しても該ワ
イヤーから剥離しないことを確認している。Next, a spiral wire made of a metal fiber having a diameter of 0.1 mm is arranged in a container, and the container is filled with the plurality of crimson-shaped gel beads having a red color, and the room temperature is reduced to 1 at room temperature.
After being left for 5 days, the spiral wire on which the gel-like gel beads were fixed was taken out from the container. The immobilized cells of the gel-like gel bead form obtained here are firmly fixed to the spiral wire, and it has been confirmed that they do not peel off from the wire even when immersed in water for 30 days.
【００３４】ここに得たイクラ状ゲルビーズ形固定化菌
体を水中に設置して長期間にわたって使用し、使用中に
栄養基質の補給を必要とする場合には、前出「発明の実
施の形態」において示した補給用パイプを、前記螺旋状
ワイヤーの螺旋内に貫通させて配管すればよい。The immobilized gel-like gel bead-shaped cells thus obtained are placed in water and used for a long period of time, and when it is necessary to replenish nutrient substrates during use, the aforementioned "Embodiment of the invention" The pipe for supply shown in "" may be penetrated into the spiral of the spiral wire.
【００３５】なお、注射筒の口径を種々変更することに
よって、その口径に応じた種々の直径のイクラ状ゲルビ
ーズを得ることができる。Incidentally, by changing the diameter of the injection cylinder in various ways, it is possible to obtain saliva gel beads having various diameters corresponding to the diameter.
【００３６】実施例３ 実施例１において用いた栄養基質（培地）と同一組成の
栄養基質１l に寒天粉末２０g を添加し、約７０℃に加
温して溶解させた後、直径１〜２mmの微細孔をもつ粒径
約３mmのヒル石粒１０g と合成繊維製フィラー１０g を
添加し、充分に撹拌混合し、次いで約４２℃に降温さ
せ、ロドシュードモナス・カプシュラータ（Ｒｈｏｄｏ
ｐｓｅｕｄｏｍｏｎａｓ ｃａｐｓｕｌａｔａ：微工研
菌寄第８７９号）菌体を接種して、Ａ液を調製した。Example 3 20 g of agar powder was added to 1 liter of a nutrient substrate having the same composition as the nutrient substrate (medium) used in Example 1 and dissolved by heating at about 70 ° C. 10 g of hillstone granules having a diameter of about 3 mm having fine pores and 10 g of synthetic fiber filler are added, mixed well with stirring, and then cooled to about 42 ° C. to obtain Rhodoseumonas capsulata (Rhodo).
Pseudomonas capsulata: No. 879 of B.I.K.) to prepare a solution A.
【００３７】１５０ml容の透明容器に、Ａ液１００mlを
投入し、室温（約２０℃）で２０分間放置してゲル状物
とし、次いで、光照明下・２０℃で７日間培養して、真
紅色を呈したゲル状固定化菌体を得た。100 ml of the solution A was placed in a 150 ml transparent container, and left at room temperature (about 20 ° C.) for 20 minutes to form a gel. The gel-like immobilized cells having a red color were obtained.
【００３８】比較例１ ヒル石粒と合成繊維製フィラーを添加しなかった以外
は、実施例３と同一条件によってゲル状固定化菌体を得
た。Comparative Example 1 Gel-like immobilized cells were obtained under the same conditions as in Example 3 except that no leech stone particles and no synthetic fiber filler were added.
【００３９】対比試験例 下水処理場の浄化放流水中のアンモニア値やＢ．Ｏ．Ｄ
値が、それぞれ２０ppm を越して３５〜４０ppm になっ
ている時期（３月〜５月）において、該浄化放流水を対
象として、実施例３で得たゲル状固定化菌体と比較例１
で得たゲル状固定化菌体とを用いて対比試験を実施し
た。Comparative Test Example Ammonia value and B.I. O. D
In the period (March to May) when the value exceeds 35 ppm and exceeds 20 ppm, the gel-like immobilized bacterial cells obtained in Example 3 and Comparative Example 1 were subjected to the purified effluent.
A comparison test was performed using the gel-like immobilized cells obtained in the above.
【００４０】アンモニア値、Ｂ．Ｏ．Ｄ値がともに３５
〜４０ppm の浄化放流水を二ラインに分流し、一方のラ
インの浄化放流水中には実施例３で得たゲル状固定化菌
体を、他方のラインの浄化放流水中には比較例１で得た
ゲル状固定化菌体を、それぞれ設置し、各ラインのアン
モニア値、Ｂ．Ｏ．Ｄ値を連続的に測定したところ、設
置後、１４日目にはいずれのラインにおいても、アンモ
ニア値、Ｂ．Ｏ．Ｄ値が１０〜１５ppm にまで低下して
浄化効果が確認できた。しかし、設置後３０日目から比
較例１のゲル状固定化菌体を設置したラインにおいては
アンモニア値、Ｂ．Ｏ．Ｄ値がともに徐々に上昇し、設
置後６０日目には、アンモニア値、Ｂ．Ｏ．Ｄ値ともに
設置前の３５〜４０ppm となり、ゲル状固定化菌体は消
滅していた。一方、実施例３のゲル状固定化菌体を設置
したラインにおいては、設置後９０日目にもアンモニア
値、Ｂ．Ｏ．Ｄ値ともに１０〜１５ppm を維持してお
り、ゲル状固定化菌体はやや小さくなってはいるがほぼ
原形を保持していた。Ammonia value, B. O. Both D values are 35
The purified effluent of about 40 ppm is divided into two lines, the gel-like immobilized bacterial cells obtained in Example 3 are obtained in one of the purified effluents, and the comparative example 1 is obtained in the other clarified effluent. The gel-immobilized cells were placed, and the ammonia value of each line, B.I. O. When the D value was measured continuously, the ammonia value, B.B. O. The D value was reduced to 10 to 15 ppm, confirming the purification effect. However, in the line where the gel-like immobilized bacterial cells of Comparative Example 1 were installed from the 30th day after the installation, the ammonia value, B.I. O. Both D values gradually increased, and on the 60th day after installation, the ammonia value, B.D. O. Both D values were 35 to 40 ppm before installation, and the gel-like immobilized cells had disappeared. On the other hand, in the line where the gel-like immobilized bacterial cells of Example 3 were installed, the ammonia value, B.B. O. The D value was maintained at 10 to 15 ppm, and the gel-like immobilized bacterial cells were slightly reduced in size, but substantially retained the original form.
【００４１】[0041]
【発明の効果】請求項１記載の発明に係るゲル状固定化
菌体は、前記多孔性粒状物を含有しているので、ゲル状
物内において特定光合成細菌菌体が該粒状物に固定され
ているから、水中において長期にわたり菌体活性が維持
でき、しかも、該粒状物の存在によってゲル状物質自体
も強化されているから、水中において長期にわたりゲル
状構造が維持できる。According to the first aspect of the present invention, the immobilized gel-like cells according to the first aspect of the present invention contain the porous particulate matter, and thus the specific photosynthetic bacterial cells are fixed to the granular matter in the gel-like substance. Therefore, the bacterial cell activity can be maintained for a long time in water, and the gel-like substance itself is strengthened by the presence of the particulate matter, so that the gel-like structure can be maintained for a long time in water.
【００４２】また、請求項２記載の発明に係るゲル状固
定化菌体は、前記繊維材料によって補強されているの
で、水中において長期にわたりゲル状構造が維持でき
る。The gel-like immobilized cells according to the second aspect of the present invention are reinforced by the fibrous material, so that the gel-like structure can be maintained in water for a long time.
【００４３】また、請求項３記載の発明に係るゲル状固
定化菌体は、前記多孔性粒状物を含有していると共に、
前記繊維材料によって補強されているので、前記の通
り、水中において長期にわたり菌体活性が維持できると
共に水中においてより長期にわたってゲル状構造が維持
できる。The gel-like immobilized cells according to the third aspect of the present invention contain the porous granular material,
Since the fiber material is reinforced, as described above, the bacterial cell activity can be maintained for a long time in water, and the gel-like structure can be maintained for a longer time in water.
【００４４】従って、本発明に係るゲル状固定化菌体は
海，河川，湖沼，池などの水質保全用途に最適のもので
あるから、本発明の産業利用性は非常に大きいといえ
る。Therefore, the gel-like immobilized bacterial cells according to the present invention are most suitable for water quality preservation applications such as seas, rivers, lakes, ponds, and the like, and thus the industrial applicability of the present invention is very large.
フロントページの続き (56)参考文献 特開 昭63−12281（ＪＰ，Ａ) 特開 昭60−137293（ＪＰ，Ａ) 特開 昭61−242580（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) C12N 11/04 Continuation of the front page (56) References JP-A-63-12281 (JP, A) JP-A-60-137293 (JP, A) JP-A-61-242580 (JP, A) (58) Fields studied (Int .Cl. 7 , DB name) C12N 11/04
Claims (2)
（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ ｃａｐｓｕｌａ
ｔａ：微工研菌寄第８７９号），ロドシュードモナス・
スフェロイデス（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ
ｓｐｈｅｒｏｉｄｅｓ：ＩＦＯ第１２２０３号）及びク
ロマチウム・ビノサム（Ｃｈｒｏｍａｔｉｕｍ ｖｉｎ
ｏｓｕｍ：微工研菌寄第８９０号）から選ばれる一種又
は二種以上の光合成細菌菌体と該菌体の栄養基質とを、
ゼラチン，海藻多糖類，ポリビニルアルコール及びアル
ギン酸ソーダから選ばれる一種又は二種以上のゲル化材
料にて固定化してなるゲル状固定化菌体において、該ゲ
ル状固定化菌体を天然繊維，合成繊維及び金属繊維から
選ばれる一種又は二種以上の繊維材料のフィラー状物に
よって補強したことを特徴とする水質保全用ゲル状固定
化菌体。1. Rhodopseudomonas capsulata
ta: No. 879)
Rhodopseudomonas
spheroides: IFO No. 12203)及 beauty Kuromachiumu-Binosamu (Chromatium vin
osum: one or two or more photosynthetic bacterial cells selected from Microtechnical Laboratory No. 890) and a nutrient substrate of the cells.
Gel-fixed cells immobilized with one or two or more gelling materials selected from gelatin, seaweed polysaccharides, polyvinyl alcohol and sodium alginate, wherein the gel-fixed cells are natural fibers or synthetic fibers And a gel-like immobilized bacterial cell for water quality preservation, reinforced with a filler material of one or more fiber materials selected from metal fibers.
（同上),ロドシュードモナス・スフェロイデス（同上）
及びクロマチウム・ビノサム（同上）から選ばれる一種
又は二種以上の光合成細菌菌体と該菌体の栄養基質と
を、ゼラチン，海藻多糖類，ポリビニルアルコール及び
アルギン酸ソーダから選ばれる一種又は二種以上のゲル
化材料にて固定化してなるゲル状固定化菌体において、
該ゲル状固定化菌体内にクロボク土粒，オオヤ石粒，炭
粒，ヒル石粒及び高分子樹脂製スポンジ粒から選ばれる
一種又は二種以上の直径１〜５mmの微細孔をもつ多孔性
粒状物を含有させると共に、当該固定化菌体を天然繊
維，合成繊維及び金属繊維から選ばれる一種又は二種以
上の繊維材料のフィラー状物によって補強したことを特
徴とする水質保全用ゲル状固定化菌体。2. Rhodoseumonas capsulata (same as above), Rhodes pseudomonas spheroides (same as above )
A nutritional substrate for one or two or more of photosynthetic bacteria cells and microbial cells selected from 及 beauty Kuromachiumu-Binosamu (ibid), gelatin, seaweed polysaccharides, one selected from polyvinyl alcohol and sodium alginate or two or more In the gel-like immobilized cells immobilized with the gelling material of
Porous granules having one or more kinds of fine pores having a diameter of 1 to 5 mm selected from black and white soil particles, oyster stone particles, charcoal particles, leech stone particles and polymer resin sponge particles in the gel-like immobilized bacteria. Characterized in that the immobilized cells are reinforced with a filler of one or more kinds of fibrous materials selected from natural fibers, synthetic fibers and metal fibers. Fungi.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7819296A JP3182076B2 (en) | 1996-03-05 | 1996-03-05 | Gel-like immobilized cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7819296A JP3182076B2 (en) | 1996-03-05 | 1996-03-05 | Gel-like immobilized cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH09238681A JPH09238681A (en) | 1997-09-16 |
| JP3182076B2 true JP3182076B2 (en) | 2001-07-03 |
Family
ID=13655140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7819296A Expired - Fee Related JP3182076B2 (en) | 1996-03-05 | 1996-03-05 | Gel-like immobilized cells |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3182076B2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7037494B2 (en) * | 1997-10-14 | 2006-05-02 | The Board Of Regents Of The University Of Texas System | Formulations and methods for insect control |
| JP2007020437A (en) * | 2005-07-13 | 2007-02-01 | Hitachi Plant Technologies Ltd | Integrally immobilizing carrier and method for producing the same |
| KR101020874B1 (en) * | 2008-11-13 | 2011-03-09 | 에스엠텍 주식회사 | Capsulation composition of algae spores for promoting attachment and growth of marine organisms |
| JP5074632B1 (en) * | 2012-02-23 | 2012-11-14 | 健 佐々木 | Method for purifying environmental media contaminated with radioactive cesium |
| JP6436525B2 (en) * | 2014-10-01 | 2018-12-12 | 株式会社レンテック | Environmental purification method |
| CN112960765A (en) * | 2021-02-07 | 2021-06-15 | 生态环境部南京环境科学研究所 | Biological permeable reactive barrier slow-release filler capable of removing trichloroethylene in underground water in situ and having core-shell structure and preparation method thereof |
-
1996
- 1996-03-05 JP JP7819296A patent/JP3182076B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH09238681A (en) | 1997-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4863110B2 (en) | Comprehensive immobilization carrier for breeding water purification, breeding water purification method and apparatus, and aquarium set | |
| AU658519B2 (en) | A method for improving bottom and water quality in water areas and a set of agents used in the method | |
| CN113754051A (en) | Biological denitrification slow-release carbon source composite material and preparation method and application thereof | |
| JP3182076B2 (en) | Gel-like immobilized cells | |
| JP3175964B2 (en) | Water environment improvement method and water environment improvement paint | |
| CN104585090B (en) | A kind of remodeling method of holothruian cultures cofferdam substrate | |
| US20220071113A1 (en) | Method for Establishing Submerged Vegetation System in Eutrophic Water Body | |
| JPH10251341A (en) | Carrier and catalyst for bioreactor | |
| CN110577315B (en) | Whole-process treatment method for phytoplankton | |
| Gikonyo et al. | Scaling-up of oxygenic photogranular system in selective-CSTR | |
| JP6538304B2 (en) | Water purification method in closed water system using microorganisms | |
| CN111748509B (en) | Microbial purifying preparation for seawater culture tail water, preparation method and application thereof | |
| JP3095862B2 (en) | Manufacturing method of materials for water environment improvement and materials for water environment improvement | |
| JP3473903B2 (en) | Paint for water environment improvement | |
| CN117965524B (en) | Preparation method and application of immobilized microorganism carrier | |
| JPS62279887A (en) | Surface immobilized anaerobic bacteria granule and treatment of waste water using same | |
| CN116903155B (en) | Water quality improver, preparation method thereof and water maintenance method | |
| Yonei et al. | Water quality improvement effect from the installation of special-glaze-applied ceramics: Benten Pond, Ichikawa, Chiba, Japan | |
| JP2008247691A (en) | Method for treating slag | |
| JP3031656B2 (en) | Microorganism growth immobilization product and method for producing the same | |
| JPH09155387A (en) | Proliferation fixing material for denitrified bacteria | |
| JPH08155476A (en) | Nitration treatment method for seawater containing ammonical nitrogen | |
| JP2903359B2 (en) | Water activator and its production method | |
| JPS6324438B2 (en) | ||
| CN116514244A (en) | Biological composite organic flocculant and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080420 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090420 Year of fee payment: 8 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090420 Year of fee payment: 8 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Year of fee payment: 9 Free format text: PAYMENT UNTIL: 20100420 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110420 Year of fee payment: 10 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Year of fee payment: 10 Free format text: PAYMENT UNTIL: 20110420 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Year of fee payment: 11 Free format text: PAYMENT UNTIL: 20120420 |
|
| LAPS | Cancellation because of no payment of annual fees |