JP3153676B2 - Sample mixing device and fluorescence detection device using the same - Google Patents
Sample mixing device and fluorescence detection device using the sameInfo
- Publication number
- JP3153676B2 JP3153676B2 JP15332193A JP15332193A JP3153676B2 JP 3153676 B2 JP3153676 B2 JP 3153676B2 JP 15332193 A JP15332193 A JP 15332193A JP 15332193 A JP15332193 A JP 15332193A JP 3153676 B2 JP3153676 B2 JP 3153676B2
- Authority
- JP
- Japan
- Prior art keywords
- reaction vessel
- stirring member
- fluorescence detection
- magnetic drive
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Description
【０００１】[0001]
【産業上の利用分野】本発明は、化学、医薬又は分析化
学等の分野において蛍光物質等の特性を測定するために
用いられる蛍光検出装置等における試料混合装置に関す
るものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample mixing device in a fluorescence detector or the like used for measuring characteristics of a fluorescent substance or the like in the fields of chemistry, medicine or analytical chemistry.
【０００２】[0002]
【従来の技術】化学、医薬又は分析化学等の分野におい
て蛍光物質等の特性を測定するために蛍光検出装置等の
開発が進んでいる。蛍光検出装置等を用いて複数の試料
を測定する場合、一般にバッチ処理と呼ばれるように、
１の試料の測定を終えた後、蛍光検出装置の試料測定部
である反応容器をホルダから外し、洗浄した後反応容器
をホルダに再度装着し次の試料の分析に備える。また、
試料と試薬との反応を促進する等の目的のため、反応容
器の内部には試料と試薬液とを均一に混合するための撹
拌部材が設けられ、撹拌部材を駆動するための駆動機構
が反応容器の外部に設けられている。さらに、夏場又は
冬場における温度変化により蛍光強度にバラツキが生ず
るため、反応容器に対して恒温水槽から恒温水等を循環
させ、温度を一定に保つようにしている。また、１回に
測定を行う試料の量がせいぜい数ｍｌと少量であり、そ
れに伴って反応容器も小型であるため、反応容器の内部
に装着し得る撹拌装置も小型でなければならず、複雑な
構造のものを用いることはできい。そのため、反応容器
の内部に独立して設けられた撹拌部材（マグネチックス
ターラバー）及び反応容器の下部に設けられ磁力により
撹拌部材を回転させる磁気駆動装置（マグネチックスタ
ーラ）等で構成された撹拌装置が用いられている。以上
のような従来の試料混合装置の構成は周知又は自明であ
るため、図示を省略する。2. Description of the Related Art In the fields of chemistry, medicine, analytical chemistry and the like, the development of fluorescence detectors and the like for measuring characteristics of fluorescent substances and the like has been progressing. When measuring a plurality of samples using a fluorescence detector or the like, as generally called batch processing,
After the measurement of one sample is completed, the reaction container, which is a sample measurement unit of the fluorescence detection device, is removed from the holder, and after cleaning, the reaction container is re-attached to the holder and prepared for the analysis of the next sample. Also,
For the purpose of accelerating the reaction between the sample and the reagent, a stirring member for uniformly mixing the sample and the reagent solution is provided inside the reaction vessel, and a driving mechanism for driving the stirring member is used for the reaction. It is provided outside the container. Further, since the fluorescence intensity varies due to a temperature change in summer or winter, constant temperature water or the like is circulated from a constant temperature water tank to the reaction vessel to keep the temperature constant. In addition, since the amount of a sample to be measured at one time is as small as several millimeters at most, and the reaction container is also small, the stirrer that can be mounted inside the reaction container must be small. It is not possible to use one with a simple structure. Therefore, a stirring member (magnetic stirrer) provided independently inside the reaction vessel and a magnetic drive device (magnetic stirrer) provided at the lower part of the reaction vessel and rotating the stirring member by magnetic force are used. The device is used. The configuration of the conventional sample mixing apparatus as described above is well known or obvious, and therefore is not shown.
【０００３】[0003]
【発明が解決しようとする課題】従来の試料混合装置を
用いて測定を行う場合、１回の試料の測定ごとに反応容
器をホルダから取外し洗浄を行わなければならず、複数
の試料の連続測定を行うことは事実上不可能であった。
また、反応容器自体が小型であるため、磁力の強力な
（大型の）磁気駆動装置を用いることができず、反応容
器内部の撹拌部材と磁気駆動装置との距離が取り付け誤
差等により所定距離よりも長くなった場合、撹拌部材に
作用する磁界の強度が弱くなり、撹拌部材の回転不良が
生ずるおそれがあった。さらに温度を一定に保たたせる
場合、中空にした反応容器ホルダへ恒温水槽から恒温水
を循環させているため、装置が大型化し、構造が複雑と
なり、保守を必要としていた。When a measurement is performed using a conventional sample mixing apparatus, the reaction vessel must be removed from the holder for each measurement of a sample, and washing must be performed. Was virtually impossible to do.
In addition, since the reaction vessel itself is small, a strong (large) magnetic drive device having a strong magnetic force cannot be used, and the distance between the stirring member inside the reaction vessel and the magnetic drive device is longer than a predetermined distance due to an installation error or the like. When the length of the stirrer becomes longer, the strength of the magnetic field acting on the stirrer becomes weaker, and there is a possibility that the rotation of the stirrer may be defective. Furthermore, when the temperature is kept constant, since the constant temperature water is circulated from the constant temperature water tank to the hollow reaction vessel holder, the apparatus becomes large, the structure becomes complicated, and maintenance is required.
【０００４】さらに、蛍光検出装置は、医療、警察、税
関等の分野においてメタンフェタミン等の覚醒剤の主成
分を検出するために用いられるため、装置が小型で且つ
容易に取扱えるものが望ましいという要請が強い。Further, since the fluorescence detection device is used for detecting the main component of a stimulant such as methamphetamine in the fields of medical care, police, customs, etc., it is required that the device be small and easy to handle. strong.
【０００５】本発明は以上のような問題点を解決するた
めになされたものであり、複数の試料の連続測定が可能
であり、撹拌不良が生ずることもなく、保守が容易で小
型の試料混合装置及びそれを用いた蛍光検出装置を提供
することを目的としている。SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and enables continuous measurement of a plurality of samples, no agitation failure, easy maintenance, and small sample mixing. It is an object of the present invention to provide an apparatus and a fluorescence detection apparatus using the same.
【０００６】[0006]
【課題を解決するための手段】本発明の試料測定装置
は、上部に注入口及び空気抜きの穴を有し、下部に排出
用のノズルを有する矩形断面を有する反応容器と、前記
反応容器の内部で且つその底部近傍に設けられ、反応容
器の外部からの磁力により回転される撹拌部材と、前記
反応容器の外部で且つ前記撹拌部材の近傍に設けられ前
記撹拌部材を回転させるための磁界を発生する磁気駆動
装置と、前記撹拌部材と前記磁気駆動装置との相対的距
離を調節するための調節機構とを具備し、 前記反応容器
の近傍に温度調節のためのペルチェ素子を設け、 前記反
応容器、磁気駆動装置、調節機構及びペルチェ素子を保
持するために表面を黒色にアルマイト処理されたアルミ
ニウム製のホルダを備えたことを特徴とする。According to the present invention, there is provided a sample measuring apparatus comprising: a reaction vessel having a rectangular cross section having an inlet and an air vent hole at an upper part and a discharge nozzle at a lower part; And a stirring member provided near the bottom thereof and rotated by a magnetic force from the outside of the reaction vessel, and a magnetic field for rotating the stirring member provided outside the reaction vessel and near the stirring member. The reaction vessel , comprising: a magnetic drive device for adjusting the relative distance between the stirring member and the magnetic drive device.
A Peltier element for temperature control provided in the vicinity of said anti
Container, magnetic drive, adjustment mechanism and Peltier element
Aluminum with black anodized surface for holding
It is characterized by having a holder made of nickel .
【０００７】また、本発明に係る蛍光検出装置は、上部
に注入口及び空気抜きの穴を有し、下部に排出用のノズ
ルを有する矩形断面を有する反応容器と、前記反応容器
の内部で且つその底部近傍に設けられ、反応容器の外部
からの磁力により回転される撹拌部材と、前記反応容器
の外部で且つ前記撹拌部材の近傍に設けられ前記撹拌部
材を回転させるための磁界を発生する磁気駆動装置と、
前記撹拌部材と前記磁気駆動装置との相対的距離を調節
するための調節機構と、前記反応容器の近傍に設けられ
前記反応容器内の試料の蛍光を検出するための蛍光検出
素子とを具備し、 前記反応容器の近傍に温度調節のため
のペルチェ素子を設け、 前記反応容器、磁気駆動装置、
調節機構及びペルチェ素子を保持するために表面を黒色
にアルマイト処理されたアルミニウム製のホルダを備え
たことを特徴とする。In addition, a fluorescence detection device according to the present invention has a reaction vessel having a rectangular cross section having an inlet and an air vent hole at an upper part, and a discharge nozzle at a lower part, and the inside and the inside of the reaction vessel. A stirring member provided near the bottom and rotated by a magnetic force from outside the reaction vessel; and a magnetic drive provided outside the reaction vessel and near the stirring member to generate a magnetic field for rotating the stirring member. Equipment and
Comprising an adjusting mechanism for adjusting the relative distance between the stirring member and the magnetic drive device and a fluorescence detection device for the provided in the vicinity of the reaction vessel for detecting the fluorescence of a sample in the reaction vessel For temperature control in the vicinity of the reaction vessel
Of the reaction vessel, a magnetic drive device,
Black surface to hold adjustment mechanism and Peltier element
With anodized aluminum holder
It is characterized by having .
【０００８】[0008]
【０００９】[0009]
【作用】以上のように構成された本発明の試料混合装置
及びそれを用いた蛍光検出装置においては、反応容器の
上部に注入口及び底部に排出用ノズルを設け、注入口か
ら洗浄液を注入し、撹拌部材を回転させて反応容器の内
部を洗浄し、廃液を排出ノズルから排出することによ
り、反応容器の内部を自動的に洗浄するように構成した
ので、複数の試料を連続的に測定することが可能であ
る。また、撹拌部材とそれを駆動するための磁気駆動装
置との相対的距離を調節機構により正規の距離に調節す
ることにより、撹拌部材は確実に且つ所定の回転数で回
転する。さらに、ペルチェ素子を設け反応容器の温度を
一定に保つように構成したので、周囲の温度条件に影響
されることなく試料の測定が行われる。さらに、ホルダ
を熱伝導率が高いアルミニウム製としたので、温度調節
に要する時間が短くなる。さらに、ホルダの表面を黒色
アルマイト処理したので、蛍光検出の際における光の散
乱が小さくなる。In the sample mixing apparatus and the fluorescence detection apparatus using the same according to the present invention, an injection port is provided at the top of the reaction vessel and a discharge nozzle is provided at the bottom, and the cleaning liquid is injected from the injection port. By rotating the stirring member to wash the inside of the reaction vessel and discharging the waste liquid from the discharge nozzle, the inside of the reaction vessel is automatically washed, so that a plurality of samples are continuously measured. It is possible. Further, by adjusting the relative distance between the stirring member and the magnetic driving device for driving the stirring member to a regular distance by the adjusting mechanism, the stirring member is surely rotated at a predetermined rotation speed. Further, since the Peltier element is provided to keep the temperature of the reaction vessel constant, the sample can be measured without being affected by the surrounding temperature conditions. Furthermore, since the holder is made of aluminum having high thermal conductivity, the time required for temperature adjustment is reduced. Further, since the surface of the holder is subjected to black alumite treatment, light scattering during fluorescence detection is reduced.
【００１０】[0010]
＜第１実施例＞本発明に係る試料混合装置及びそれを用
いた蛍光検出装置を、それらの好適な第１の実施例を示
す図１、図２及び図３を用いて説明する。図１中（ａ）
は試料混合装置又は蛍光検出装置の構成を示す正面図、
（ｂ）はその側面図である。図２は複数の試料を連続的
に測定するために構成された試料混合装置又は蛍光検出
装置の概略構成を示すブロック図である。図３は図１及
び図２における反応容器の詳細な構成を示す斜視図であ
る。<First Embodiment> A sample mixing apparatus according to the present invention and a fluorescence detection apparatus using the same will be described with reference to FIGS. 1, 2 and 3 showing preferred first embodiments. (A) in FIG.
Is a front view showing the configuration of the sample mixing device or the fluorescence detection device,
(B) is a side view thereof. FIG. 2 is a block diagram illustrating a schematic configuration of a sample mixing device or a fluorescence detection device configured to continuously measure a plurality of samples. FIG. 3 is a perspective view showing a detailed configuration of the reaction vessel in FIG. 1 and FIG.
【００１１】図１において、本発明の試料混合装置又は
蛍光検出装置５は、アルミニウム製のホルダ１０と、ホ
ルダ１０に固定された反応容器１１と、ホルダ１０に設
けられた調節機構（調節ねじ）１２を介して反応容器１
１の外部で且つ下部に設けられた磁気駆動装置（マグネ
チックスターラ）７と、反応容器１１の内部で且つ底部
近傍に設けられた撹拌部材（マグネチックスターラバ
ー）１７と、反応容器１１の近傍に設けられた蛍光検出
素子（又は他の検出素子）１３等で構成されている。図
３に示すように、反応容器１１の上面１１ａには注入口
１１ｃ及び空気抜きの穴１１ｄが設けられており、底部
には排出ノズル１１ｂが設けられている。反応容器１１
は、例えば１０ｍｍ×１０ｍｍ×４５ｍｍの（矩形断面
を有する）角型セルであり、磁気駆動装置７は、例えば
直径１２ｍｍ×長さ２２ｍｍの円柱形である。図１
（ｂ）に示すように、磁気駆動装置７はホルダ１０の底
部に設けられた調節機構（調節ねじ）１２により上下に
調節できるように固定されている。これにより、反応容
器１１の内部に設けられた撹拌部材１７との相対的距離
を最大限３ｍｍにまで近接させることができる。なお、
温度を一定に保ために温度制御を行う場合を考慮して、
ホルダ１０の材料として熱電導性の良いアルミニウムを
使用し、また蛍光検出装置として使用する場合を考慮し
て、ホルダ１０の表面はアルマイト処理により黒色に着
色されている。In FIG. 1, a sample mixing apparatus or a fluorescence detecting apparatus 5 of the present invention comprises an aluminum holder 10, a reaction vessel 11 fixed to the holder 10, and an adjusting mechanism (adjusting screw) provided on the holder 10. Reaction vessel 1 through 12
A magnetic driving device (magnetic stirrer) 7 provided outside and at the bottom of the reactor 1; a stirring member (magnetic stir bar) 17 provided inside the reaction vessel 11 and near the bottom; , A fluorescence detection element (or another detection element) 13 or the like. As shown in FIG. 3, an inlet 11c and an air vent hole 11d are provided on the upper surface 11a of the reaction vessel 11, and a discharge nozzle 11b is provided at the bottom. Reaction vessel 11
Is a rectangular cell (having a rectangular cross section) of, for example, 10 mm × 10 mm × 45 mm, and the magnetic drive device 7 is, for example, a cylindrical shape having a diameter of 12 mm × a length of 22 mm. FIG.
As shown in (b), the magnetic drive device 7 is fixed so that it can be adjusted up and down by an adjustment mechanism (adjustment screw) 12 provided at the bottom of the holder 10. Thereby, the relative distance to the stirring member 17 provided inside the reaction vessel 11 can be reduced to a maximum of 3 mm. In addition,
Considering the case of performing temperature control to keep the temperature constant,
The surface of the holder 10 is colored black by alumite treatment in consideration of the case where aluminum having good thermal conductivity is used as the material of the holder 10 and that the holder 10 is used as a fluorescence detector.
【００１２】図２に示すように、反応容器１１の上部に
設けられた例えば２つの注入口１１ｃはそれぞれシリン
ジ型のポンプ１及び２に接続され、排出ノズル１１ｂは
排出ポンプ３を介して廃液貯蔵容器４に接続されてい
る。ポンプ１は例えば試薬である抗体液の注入量を制御
するために用いられ、またポンプ２は試料液の注入量を
制御するために用いられる。また、磁気駆動装置７には
制御装置６が接続されている。As shown in FIG. 2, for example, two inlets 11c provided at the upper part of the reaction vessel 11 are respectively connected to syringe type pumps 1 and 2, and a discharge nozzle 11b is connected to a waste liquid storage via a discharge pump 3. It is connected to the container 4. The pump 1 is used, for example, for controlling the injection amount of an antibody solution as a reagent, and the pump 2 is used for controlling the injection amount of a sample solution. The control device 6 is connected to the magnetic drive device 7.
【００１３】＜第２実施例＞次に、本発明に係る試料混
合装置及びそれを用いた蛍光検出装置を、それらの好適
な第２の実施例を示す図４及び図５を用いて説明する。
図４中（ａ）は試料混合装置又は蛍光検出装置の構成を
示す正面図、（ｂ）はその側面図である。図５は複数の
試料を連続的に測定するために構成された試料混合装置
又は蛍光検出装置の概略構成を示すブロック図である。
なお、上記第１の実施例と同じ番号を付した部材は実質
的に同じものであるため、その説明を省略する。<Second Embodiment> Next, a sample mixing apparatus according to the present invention and a fluorescence detection apparatus using the same will be described with reference to FIGS. 4 and 5 showing preferred second embodiments thereof. .
4A is a front view showing the configuration of the sample mixing device or the fluorescence detection device, and FIG. 4B is a side view thereof. FIG. 5 is a block diagram illustrating a schematic configuration of a sample mixing device or a fluorescence detection device configured to continuously measure a plurality of samples.
Note that members denoted by the same reference numerals as those in the first embodiment are substantially the same, and a description thereof will be omitted.
【００１４】図４及び図５において、ホルダ１０にはペ
ルチェ素子３２及び放熱フィン３３が設けられている。
温度制御装置であるペルチェ素子３２は、大きさ２３ｍ
ｍ×２３ｍｍであり、電源電流を一定にして制御され
る。放熱フィン３３は温度制御効率を高めるためアルミ
ニウム製のものを用いた。4 and 5, the holder 10 is provided with a Peltier element 32 and a radiation fin 33.
The Peltier element 32 which is a temperature control device has a size of 23 m.
m × 23 mm, and is controlled with the power supply current kept constant. The radiating fins 33 were made of aluminum in order to increase the temperature control efficiency.
【００１５】＜第１実験例＞上記第１及び第２の実施例
に係る試料混合装置又は蛍光検出装置を用いて、撹拌部
材１７の回転、反応容器１１からの廃液の排出状況、及
び第２の実施例における反応容器１１内の試料等の液体
の温度制御について測定を行った。<First Experimental Example> Using the sample mixing apparatus or the fluorescence detecting apparatus according to the first and second embodiments, the rotation of the stirring member 17, the discharge of waste liquid from the reaction vessel 11, and the second Measurement was performed on the temperature control of the liquid such as the sample in the reaction vessel 11 in the example of (1).
【００１６】まず、撹拌部材１７の回転に関して、反応
容器１１の内部に液体が存在している場合と存在してい
ない場合について、それぞれ撹拌部材１７の回転数を８
００ｒｐｍに設定し、制御装置６のオン／オフにより磁
気駆動装置７を制御し、撹拌部材１７が良好に（円滑
に）回転するかどうかを確認した（２０回以上）。その
結果、いずれの場合でも撹拌部材１７は良好に回転する
ことが確認された。First, regarding the rotation of the stirring member 17, the rotation speed of the stirring member 17 is set to 8 when the liquid exists inside the reaction vessel 11 and when the liquid does not exist inside the reaction container 11.
It was set to 00 rpm, and the magnetic drive 7 was controlled by turning on / off the control device 6, and it was confirmed whether the stirring member 17 rotated well (smoothly) (20 times or more). As a result, it was confirmed that the stirring member 17 rotated favorably in each case.
【００１７】次に、廃液の排出状況に関して、注入口１
１ｃにシリンジポンプ１又は２を接続し、反応容器１１
の内部に１．５ｍｌの蒸留水を注入し、次に排出ポンプ
３を駆動して蒸留水を廃液貯蔵容器４に排出した。この
実験を３度繰り返し行ったところ、反応容器１１内には
約50μｌの残液があった。この値は、注入量の約４％で
あり、許容誤差の範囲内にある。すなわち、この程度の
残液であれば自動洗浄としては十分であり、連続的に試
料測定を行うことが可能であることが確認された。Next, regarding the discharge state of the waste liquid,
1c is connected to the syringe pump 1 or 2,
Then, 1.5 ml of distilled water was injected into the container, and then the discharge pump 3 was driven to discharge the distilled water to the waste liquid storage container 4. When this experiment was repeated three times, about 50 μl of the residual liquid was found in the reaction vessel 11. This value is about 4% of the injection volume and is within tolerance. That is, it was confirmed that the residual liquid of this degree was sufficient for automatic cleaning, and it was possible to continuously perform sample measurement.
【００１８】さらに、反応容器１１の内部にあらかじめ
２５℃に保温しておいた蒸留水１．５ｍｌを注入し、ペ
ルチェ素子３２の駆動開始３０分後における反応容器１
１の液温を測定したところ２５℃±０．５℃であった。
すなわち、ペルチェ素子３２を用いて反応容器１１の内
部の液体の温度を一定に保つことが可能であることが確
認された。Further, 1.5 ml of distilled water kept at 25 ° C. in advance was poured into the inside of the reaction vessel 11, and 30 minutes after the start of driving of the Peltier device 32,
The liquid temperature of No. 1 was 25 ° C. ± 0.5 ° C.
That is, it was confirmed that the temperature of the liquid inside the reaction vessel 11 can be kept constant by using the Peltier device 32.
【００１９】＜第２実験例＞上記本発明に係る試料混合
装置又は蛍光検出装置を用いて抗メタンフェタミンモノ
クローナル抗体を用いたメタンフェタミン（ＭＡ）の測
定を蛍光増強法により測定した。その結果を図６に示
す。蛍光検出素子１３として日本分光社製のＦＰ−５２
０を用いた。まず、反応容器１１の温度を２５℃にした
後、抗体液用のポンプ１を駆動して１×１０-7Ｍ（ｍｏ
ｌ／ｌ）抗体液１．５ｍｌを反応容器１１の内部に注入
し、撹拌部材１７を回転させ抗体液（試薬）を撹拌させ
た後、蛍光を測定した（励起波長：２８０ｎｍ、測定波
長：３４０ｎｍ）。次に、撹拌部材１７を回転させた状
態で試料液用のポンプ２を駆動して１×１０-6Ｍのメタ
ンフェタミン８０μｌを反応容器１１の内部に注入し、
再度蛍光を測定した（励起波長：２８０ｎｍ、測定波
長：３４０ｎｍ）。その結果、図６に示すように蛍光強
度が１０％増加したことが確認された。以上の測定に要
した時間は約６０秒であった。次に、排出用ポンプ３を
作動させ、被測定液を廃液貯蔵容器４に排出し、抗体液
用ポンプ１を駆動して抗体液２．５ｍｌを反応容器１１
の内部に注入し、反応容器１１の内部に残留した廃液
（メタンフェタミン）を洗浄した。その後、上記手順に
従って、次の試料の測定を行った。<Second Experimental Example> The methamphetamine (MA) using an anti-methamphetamine monoclonal antibody was measured using the sample mixing apparatus or the fluorescence detection apparatus according to the present invention by a fluorescence enhancement method. FIG. 6 shows the result. FP-52 manufactured by JASCO Corporation as the fluorescence detecting element 13
0 was used. First, after the temperature of the reaction vessel 11 was set to 25 ° C., the antibody solution pump 1 was driven to drive the reaction solution to 1 × 10 −7 M (mo).
1 / l) Inject 1.5 ml of the antibody solution into the reaction vessel 11, rotate the stirring member 17 to stir the antibody solution (reagent), and then measure the fluorescence (excitation wavelength: 280 nm, measurement wavelength: 340 nm) ). Next, while the stirring member 17 is being rotated, the sample liquid pump 2 is driven to inject 80 μl of 1 × 10 −6 M methamphetamine into the reaction vessel 11,
The fluorescence was measured again (excitation wavelength: 280 nm, measurement wavelength: 340 nm). As a result, it was confirmed that the fluorescence intensity increased by 10% as shown in FIG. The time required for the above measurement was about 60 seconds. Next, the discharge pump 3 is operated to discharge the liquid to be measured into the waste liquid storage container 4.
And the waste liquid (methamphetamine) remaining inside the reaction vessel 11 was washed. Thereafter, the next sample was measured according to the above procedure.
【００２０】[0020]
【発明の効果】以上のように、本発明の試料混合装置及
びそれを用いた蛍光検出装置によれば、反応容器の上部
に注入口及び底部に排出用ノズルを設け、注入口から洗
浄液を注入し、撹拌部材を回転させて反応容器の内部を
洗浄し、廃液を排出ノズルから排出することにより、反
応容器の内部を自動的に洗浄するように構成したので、
複数の試料を連続的に測定することが可能である。ま
た、調節機構により撹拌部材とそれを駆動するための磁
気駆動装置との相対的距離を調節することができ、撹拌
部材を確実に且つ所定の回転数で回転することができ
る。さらに、ペルチェ素子を設けたので、反応容器の温
度を一定に保つことができ、周囲の温度条件に影響され
ることなく試料の測定が行うことができる。さらに、ホ
ルダを熱伝導率が高いアルミニウム製としたので、温度
調節に要する時間を短くすることができる。さらに、ホ
ルダの表面を黒色アルマイト処理したので、蛍光検出の
際における光の散乱を小さくし、測定精度を向上させる
ことができる。As described above, according to the sample mixing apparatus of the present invention and the fluorescence detecting apparatus using the same, the inlet is provided at the top of the reaction vessel and the discharge nozzle is provided at the bottom, and the cleaning liquid is injected from the inlet. Then, by rotating the stirring member to wash the inside of the reaction vessel and discharging the waste liquid from the discharge nozzle, the inside of the reaction vessel is automatically washed, so that
It is possible to measure a plurality of samples continuously. Further, the relative distance between the stirring member and the magnetic drive device for driving the stirring member can be adjusted by the adjusting mechanism, and the stirring member can be surely rotated at a predetermined rotation speed. Further, since the Peltier element is provided, the temperature of the reaction vessel can be kept constant, and the measurement of the sample can be performed without being affected by the surrounding temperature conditions. Further, since the holder is made of aluminum having high thermal conductivity, the time required for temperature adjustment can be shortened. Furthermore, since the surface of the holder is subjected to black alumite treatment, light scattering during fluorescence detection can be reduced, and measurement accuracy can be improved.
【図面の簡単な説明】[Brief description of the drawings]
【図１】（ａ）は本発明の試料混合装置又は蛍光検出装
置の第１の実施例の構成を示す正面図、（ｂ）はその側
面図FIG. 1 (a) is a front view showing the configuration of a first embodiment of a sample mixing device or a fluorescence detection device of the present invention, and FIG. 1 (b) is a side view thereof.
【図２】複数の試料を連続的に測定するために構成され
た第１の実施例に係る試料混合装置又は蛍光検出装置の
概略構成を示すブロック図FIG. 2 is a block diagram illustrating a schematic configuration of a sample mixing device or a fluorescence detection device according to a first embodiment configured to continuously measure a plurality of samples.
【図３】図１及び図２における反応容器の詳細な構成を
示す斜視図FIG. 3 is a perspective view showing a detailed configuration of a reaction vessel in FIGS. 1 and 2;
【図４】（ａ）は本発明の試料混合装置又は蛍光検出装
置の第２の実施例の構成を示す正面図、（ｂ）はその側
面図FIG. 4 (a) is a front view showing the configuration of a second embodiment of the sample mixing device or the fluorescence detection device of the present invention, and FIG. 4 (b) is a side view thereof.
【図５】複数の試料を連続的に測定するために構成され
た第２の実施例に係る試料混合装置又は蛍光検出装置の
概略構成を示すブロック図FIG. 5 is a block diagram illustrating a schematic configuration of a sample mixing device or a fluorescence detection device according to a second embodiment configured to continuously measure a plurality of samples.
【図６】本発明の各実施例に係る蛍光検出装置を用いて
メタンフェタミンの蛍光強度を測定した結果を示す図FIG. 6 is a diagram showing the results of measuring the fluorescence intensity of methamphetamine using the fluorescence detection device according to each example of the present invention.
１ 抗体液用ポンプ ２ 試料液用ポンプ ３ 排出用ポンプ ４ 廃液貯蔵容器 ５ 試料混合装置又は蛍光検出装置 ６ 制御装置 ７ 磁気駆動装置 １０ ホルダ １１ 反応容器 １１ｂ 排出ノズル １１ｃ 注入口 １２ 調節機構（調節ねじ） １３ 蛍光検出素子 １７ 撹拌部材 ３２ ペルチェ素子 ３３ 放熱フィン DESCRIPTION OF SYMBOLS 1 Pump for antibody liquid 2 Pump for sample liquid 3 Pump for discharge 4 Waste liquid storage container 5 Sample mixing device or fluorescence detection device 6 Control device 7 Magnetic drive device 10 Holder 11 Reaction container 11b Discharge nozzle 11c Inlet 12 Adjustment mechanism (adjustment screw 13) Fluorescence detection element 17 Stirring member 32 Peltier element 33 Heat radiation fin
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭58−62564（ＪＰ，Ａ) 実開 平５−3985（ＪＰ，Ｕ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 35/00 - 35/10 B01F 13/08 G01N 21/03 G01N 21/64 ──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-58-62564 (JP, A) Japanese Utility Model Application Hei 5-3985 (JP, U) (58) Fields investigated (Int. Cl. 7 , DB name) G01N 35/00-35/10 B01F 13/08 G01N 21/03 G01N 21/64
Claims (2)
下部に排出用のノズルを有する矩形断面を有する反応容
器と、前記反応容器の内部で且つその底部近傍に設けら
れ、反応容器の外部からの磁力により回転される撹拌部
材と、前記反応容器の外部で且つ前記撹拌部材の近傍に
設けられ前記撹拌部材を回転させるための磁界を発生す
る磁気駆動装置と、前記撹拌部材と前記磁気駆動装置と
の相対的距離を調節するための調節機構とを具備し、 前記反応容器の近傍に温度調節のためのペルチェ素子を
設け、 前記反応容器、磁気駆動装置、調節機構及びペルチェ素
子を保持するために表面を黒色にアルマイト処理された
アルミニウム製のホルダを備えたことを特徴とする 試料
測定装置。1. An injection port and an air vent hole are provided at an upper part,
A reaction vessel having a rectangular cross section having a discharge nozzle at a lower portion, a stirring member provided inside the reaction vessel and near the bottom thereof, and rotated by a magnetic force from outside the reaction vessel; A magnetic drive device that is provided near the stirring member and generates a magnetic field for rotating the stirring member, and an adjusting mechanism for adjusting a relative distance between the stirring member and the magnetic drive device. And a Peltier element for temperature control in the vicinity of the reaction vessel.
Provided, the reaction vessel, a magnetic drive, an adjustment mechanism and Peltier element
Black anodized surface to hold offspring
A sample measuring device comprising an aluminum holder .
下部に排出用のノズルを有する矩形断面を有する反応容
器と、前記反応容器の内部で且つその底部近傍に設けら
れ、反応容器の外部からの磁力により回転される撹拌部
材と、前記反応容器の外部で且つ前記撹拌部材の近傍に
設けられ前記撹拌部材を回転させるための磁界を発生す
る磁気駆動装置と、前記撹拌部材と前記磁気駆動装置と
の相対的距離を調節するための調節機構と、前記反応容
器の近傍に設けられ前記反応容器内の試料の蛍光を検出
するための蛍光検出素子とを具備し、 前記反応容器の近傍に温度調節のためのペルチェ素子を
設け、 前記反応容器、磁気駆動装置、調節機構及びペルチェ素
子を保持するために表面を黒色にアルマイト処理された
アルミニウム製のホルダを備えたことを特徴とする 蛍光
検出装置。2. It has an inlet and an air vent hole at the top,
A reaction vessel having a rectangular cross section having a discharge nozzle at a lower portion, a stirring member provided inside the reaction vessel and near the bottom thereof, and rotated by a magnetic force from outside the reaction vessel; And a magnetic drive device that is provided near the stirring member and generates a magnetic field for rotating the stirring member; an adjustment mechanism for adjusting a relative distance between the stirring member and the magnetic drive device; provided in the vicinity of the reaction vessel and a fluorescence detection device for detecting the fluorescence of a sample in the reaction vessel, the Peltier device for temperature control in the vicinity of the reaction vessel
Provided, the reaction vessel, a magnetic drive, an adjustment mechanism and Peltier element
Black anodized surface to hold offspring
A fluorescence detection device comprising an aluminum holder .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15332193A JP3153676B2 (en) | 1993-06-24 | 1993-06-24 | Sample mixing device and fluorescence detection device using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15332193A JP3153676B2 (en) | 1993-06-24 | 1993-06-24 | Sample mixing device and fluorescence detection device using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0712821A JPH0712821A (en) | 1995-01-17 |
| JP3153676B2 true JP3153676B2 (en) | 2001-04-09 |
Family
ID=15559943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15332193A Expired - Fee Related JP3153676B2 (en) | 1993-06-24 | 1993-06-24 | Sample mixing device and fluorescence detection device using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3153676B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230030762A (en) * | 2021-08-26 | 2023-03-07 | 에스케이매직 주식회사 | Apparatus for cultivating plants |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2544455T3 (en) * | 1997-02-28 | 2015-08-31 | Cepheid | Mounting for chemical reaction with heat exchange, optically interrogated |
| JP2000319009A (en) * | 1999-03-08 | 2000-11-21 | Idemitsu Petrochem Co Ltd | Separation of non-lithium hydroxide solid material |
| JP3641723B2 (en) | 2003-09-30 | 2005-04-27 | ダイキン工業株式会社 | Indoor unit for air conditioner and method for assembling indoor unit for air conditioner |
| ES2615355T3 (en) | 2003-09-30 | 2017-06-06 | Daikin Industries, Ltd | Indoor unit of air conditioner |
| KR100768160B1 (en) * | 2006-05-16 | 2007-10-17 | 위니아만도 주식회사 | A front cover having a supporting bar of in-door unit |
| CN108838158A (en) * | 2018-07-16 | 2018-11-20 | 精准（天津）生物科技有限公司 | Micropore plate cleaning device for immunoassay experiment and use method |
-
1993
- 1993-06-24 JP JP15332193A patent/JP3153676B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230030762A (en) * | 2021-08-26 | 2023-03-07 | 에스케이매직 주식회사 | Apparatus for cultivating plants |
| KR102662041B1 (en) * | 2021-08-26 | 2024-04-29 | 에스케이매직 주식회사 | Apparatus for cultivating plants |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0712821A (en) | 1995-01-17 |
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