JP3075602B2 - Electrophoresis device - Google Patents
Electrophoresis deviceInfo
- Publication number
- JP3075602B2 JP3075602B2 JP03233489A JP23348991A JP3075602B2 JP 3075602 B2 JP3075602 B2 JP 3075602B2 JP 03233489 A JP03233489 A JP 03233489A JP 23348991 A JP23348991 A JP 23348991A JP 3075602 B2 JP3075602 B2 JP 3075602B2
- Authority
- JP
- Japan
- Prior art keywords
- electrophoresis
- buffer tank
- molecular sieve
- buffer
- detection target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Description
【０００１】[0001]
【産業上の利用分野】本発明は電気泳動装置、特に、試
料導入部が改良されたＤＮＡ等の分離検出に有効なゲル
電気泳動装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an electrophoresis apparatus and, more particularly, to a gel electrophoresis apparatus having an improved sample introduction portion and effective for separation and detection of DNA and the like.
【０００２】[0002]
【従来の技術】従来、ＤＮＡシーケンシングをはじめ、
ＤＮＡの分離検出には平板型ゲル（スラブゲル）が用い
られてきた。この装置でのＤＮＡの最小検出量は測定領
域内のＤＮＡ濃度と体積に依存する。従って、微量の試
料を有効に検出するためにはＤＮＡバンドの体積を小さ
くする必要がある。これを実現する方法としてキャピラ
リー電気泳動法が提案されておりＤＮＡ解析にも使用さ
れている（Anal. Chem.62, 900-903,(1990)）。2. Description of the Related Art Conventionally, including DNA sequencing,
Plate-type gels (slab gels) have been used for separation detection of DNA. The minimum detection amount of DNA in this device depends on the DNA concentration and volume in the measurement area. Therefore, it is necessary to reduce the volume of the DNA band in order to effectively detect a small amount of sample. As a method for realizing this, a capillary electrophoresis method has been proposed and used for DNA analysis (Anal. Chem. 62, 900-903, (1990)).
【０００３】キャピラリー電気泳動に現在用いられてい
る装置は図５にその概念図が示されるように、バッファ
ー液で満たされた第１と第２のバッファー槽１０１、１
０２とを有し、二つのバッファー槽内には泳動電界電位
を与えるため高電圧電源Ｂからの電極１０３、１０４が
装着されている。また、二つの槽内のバッファー液はキ
ャピラリー１０５を介して連通し得るようにされてお
り、該キャピラリーの適宜箇所に検出部１０６が位置し
ている。この装置を用いてＤＮＡ等の分離検出を行うに
当たっては、バッファー液で満たされたバッファー槽か
らキャピラリーの先端を離脱させ、外部に位置する試料
溜Ｓにその先端を挿入して所定量の試料をキャピラリー
の先端に導入した後、再びキャピラリーの先端をバッフ
ァー槽内のバッファー液内に浸漬させる。しかる後、バ
ッファー槽間に泳動電極を与え試料を電気泳動させ分析
を行う。[0003] The apparatus currently used for capillary electrophoresis is shown in FIG.
First and second buffer over bath filled with over liquid 101,1
And a 02, electrodes 103 and 104 from the high voltage power supply B to provide the electrophoretic field potential is attached to the two buffers over tank. The buffer over liquid in the two tanks is key <br/> through Yapirari over 105 are adapted to be communicated with, the detection unit 106 to the appropriate position of the calibration peak rally is located. In performing the separation and detection of DNA or the like using this device, to disengage the front end of the calibration peak rally from the buffer over tank filled with buffer over liquid, and insert the tip into the sample reservoir S located outside after introducing a predetermined amount of the sample to the tip of the calibration peak rally <br/>, it is immersed again tip of calibration peak rally the buffer <br/> buffer over liquid within § chromatography tank. Thereafter, an analysis was electrophoresed samples gave migration electrode between bar <br/> Ffa over bath.
【０００４】この種のキャピラリー電気泳動において
は、通常試料はμｌのオーダの試料溜から、電界注入に
よりｎｌ程度がキャピラリー内に入り分析される。In this type of capillary electrophoresis, usually about nl of a sample enters a capillary from a sample reservoir of the order of μl by electric field injection and is analyzed.
【０００５】[0005]
【発明が解決しようとする課題】上記従来技術ではキャ
ピラリーに注入される試料量はわずかであるが、注入量
の１００倍近い試料を試料溜に必要とする難点があっ
た。このため、分離検出に必要な実際の試料の必要最小
量は必ずしも小さくなく、極微量成分の分析には難点が
あった。In the above prior art, the amount of the sample injected into the capillary is small, but there is a drawback that a sample that is nearly 100 times the injected amount is required in the sample reservoir. For this reason, the required minimum amount of the actual sample required for separation and detection is not necessarily small, and there has been a difficulty in analyzing trace components.
【０００６】本発明の目的は、試料溜中にあるＤＮＡ等
の分析対象試料のほぼ全量をいったん濃縮し、その後に
分離泳動部に泳動させることにより、分析検出に実際に
必要な極微量の試料とほぼ同量の試料を試料溜に提供す
るだけで、十分な分離検出を行い得る電気泳動によるＤ
ＮＡ等の試料分離分析装置を提供することにある。[0006] An object of the present invention is to concentrate almost all of a sample such as DNA in a sample reservoir once and then migrate it to a separation electrophoresis section, thereby obtaining a very small amount of sample actually required for analysis and detection. By providing an approximately same amount of sample to the sample reservoir, D by electrophoresis that can perform sufficient separation and detection
An object of the present invention is to provide a sample separation / analysis device such as NA.
【０００７】[0007]
【課題を解決するための手段】上記目的を達成するため
に、本発明は、試料注入部、分離泳動部、検出部、及び
泳動用高圧電源供給部とからなる蛍光検出型電気泳動装
置において、該試料注入部は相互に細管部分で連結され
た二つのバッファー槽及び該細管部分に位置する検出対
象物は通過し得ないが検出対象物より小さいサイズの物
質は通過し得る第１の分子ふるい膜とを有していると共
に、該分離泳動部は該分子ふるい膜の近傍上流側に開口
しており、かつ泳動用高圧電源供給部は該二つのバッフ
ァー槽間及び二つのバッファー槽間の一方と分離泳動部
間とに切り替え自在に接続していることを特徴とする電
気泳動装置を開示する。In order to achieve the above object, the present invention provides a fluorescence detection type electrophoresis apparatus comprising a sample injection section, a separation and migration section, a detection section, and a high voltage power supply section for migration. sample injection portion to the first molecule capable of passing material detection object located concatenated two buffer over bath and capillary tubes part tubule portion is smaller than the detection target but not pass size to each other together and a sieve membrane, the separation lane area is open in the vicinity upstream of the molecule sieve membrane, and high-voltage power supply unit for electrophoresis is and between said two buffer <br/> § over bath discloses an electrophoresis apparatus characterized by connecting freely switch and between one and the separation electrophoresis unit between two buffer over bath.
【０００８】該細管部分のより上流側に検出対象物は通
過し得るが検出対象物より大きいサイズの物質の通過は
阻止する第２の分子ふるい膜を設け、該分離泳動部を第
２と第１の分子ふるい膜間の該細管部分に開口させるこ
とにより、より目的を達成することができる。分離泳動
部はバッファー液を満たした中空キャピラリーであって
よく、またガラスキャピラリー内部に形成されたゲルで
あってもよい。さらに、２枚のガラス平板の間に形成さ
れた平板型ゲルであってもよい。[0008] A second molecular sieve membrane is provided on the upstream side of the capillary portion to allow the detection target to pass therethrough, but to block the passage of a substance having a size larger than the detection target. The object can be further achieved by opening the capillary portion between the one molecular sieve membranes. Separating lane area may be hollow calibration pin rally filled with buffer over liquid, or may be a gel formed within Garasukya pin rally. Further, a flat gel formed between two glass flat plates may be used.
【０００９】また、分子ふるい膜面積を該分離泳動部の
断面積とほぼ等しいかあるいはより小さな面積に制限す
る手段を具備することにより、より目的は達成される。Further, the object can be further achieved by providing a means for limiting the area of the molecular sieve membrane to an area substantially equal to or smaller than the cross-sectional area of the separation / phoresis section.
【００１０】[0010]
【作用】本発明による電気泳動装置の好ましい態様にお
いては、試料注入部は、第１及び第２のバッファー槽
と、第２のバッファー槽内の底部に位置する試料注入ウ
ェルと、分子サイズを選択分離する分子ふるい膜と、二
つのバッファー槽相互間を連結する細管によって構成さ
れている。また、上記分子ふるい膜は好ましくは第１の
バッファー槽と第２のバッファー槽とを連結する細管の
途中に設けられる。試料の分析にあたり、まず試料を上
記第２のバッファー槽内の底部に設けた試料注入ウェル
に注入し、上記第１及び第２のバッファー槽をバッファ
ー液で満たす。それにより上記細管内はバッファー液で
満たされるとともに、該分子ふるい膜近傍にその入り口
を開口している分離泳動部もバッファー液で満たされ
る。In a preferred embodiment of the electrophoresis apparatus according to the present invention, the sample injecting section is configured by selecting a first and a second buffer tank, a sample injecting well located at the bottom of the second buffer tank, and a molecular size. It consists of a molecular sieve membrane to be separated and a capillary tube connecting the two buffer tanks. Further, the molecular sieve membrane is preferably provided in the middle of a thin tube connecting the first buffer tank and the second buffer tank. In analyzing the sample, first, the sample is injected into a sample injection well provided at the bottom in the second buffer tank, and the first and second buffer tanks are filled with a buffer solution. As a result, the inside of the thin tube is filled with the buffer solution, and the separation electrophoresis part whose entrance is opened near the molecular sieve membrane is also filled with the buffer solution.
【００１１】この状態で上記泳動用の電極間に電位を与
えて、第１及び第２のバッファー槽間での電気泳動を可
能とする。分子ふるい膜はバッファー液中の分子サイズ
の小さい塩は通すが、分子サイズの大きなＤＮＡ分子は
通さないものが選択され、従って、第１及び第２のバッ
ファー槽間での電気泳動によって、上記分子ふるい膜の
面あるいはその近傍に試料は濃縮される。In this state, a potential is applied between the electrodes for electrophoresis to enable electrophoresis between the first and second buffer tanks. The molecular sieve membrane is selected so that the salt having a small molecular size in the buffer solution is allowed to pass through but the DNA molecule having a large molecular size is not allowed to pass through. Therefore, the electrophoresis between the first and second buffer tanks allows the above molecular sieve to be formed. The sample is concentrated on or near the surface of the sieving membrane.
【００１２】このようにして濃縮されたＤＮＡ等の試料
は分離泳動部のキャピラリー等に直結した領域に保持さ
れた後、分離泳動部のキャピラリー等内を電気泳動する
ので、ほぼ全試料を無駄なく分離、検出の測定に使用す
ることができ、計測に必要な最小量を少なくできる。な
お、本発明において「細管部分」というときは、単に細
管そのもののみならず、以下の実施例、特に第２、３、
４の実施例に示すように二つのバッファー槽間を連通す
る管路部分のすべてをいうものとする。The sample such as DNA concentrated in this manner is held in a region directly connected to the capillary or the like in the separation and migration section, and then electrophoresed in the capillary or the like in the separation and migration section. It can be used for measurement of separation and detection, and the minimum amount required for measurement can be reduced. In the present invention, the term "capillary portion" refers not only to the capillaries themselves, but also to the following examples, particularly to the second, third, and third embodiments.
As shown in the fourth embodiment, the term refers to all the pipe sections communicating between the two buffer tanks.
【００１３】[0013]
【実施例】以下、本発明による電気泳動装置を添付の図
面を参照したいくつかの実施例に基づきより詳細に説明
する。図１は、第一の実施例を示しており、その装置を
ＤＮＡ断片の解析を行う場合を例にとって説明する。図
において、ａは試料注入部であり、第１のバッファー槽
１及び第２のバッファー槽２とが隔壁３を介して位置し
ており、一方のバッファー槽、図においてはバッファー
槽２の底部には試料注入ウェル４が設けられている。試
料注入ウェル４の底部と他方のバッファー槽、図におい
てはバッファー槽１の底部とは細管５により連通されて
いて、該細管５のバッファー槽１の底部近傍には分子ふ
るい膜６が介装されている。この分子ふるい膜６は分子
サイズの小さい塩は通すが、分子サイズの大きなＤＮＡ
分子は通さない物性を有するものであって、例えば、セ
ルロースアセテート膜やポリエーテルスルフォン膜など
が利用できる。DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, an electrophoresis apparatus according to the present invention will be described in more detail with reference to the accompanying drawings. FIG. 1 shows a first embodiment, and the apparatus will be described by way of example in which a DNA fragment is analyzed. In FIG., A is a sample injection part, a first buffer over tank 1 and the second buffer over tank 2 is positioned through the septum 3, one buffer over tank buffer over in FIG. < A sample injection well 4 is provided at the bottom of the tank 2. Bottom and the other buffer over bath sample injection well 4, is the bottom of the buffer over tank 1 have been communicated by capillary 5 in the figure, near the bottom of the buffer over tank 1 of capillary tubes 5 molecular sieve film 6 It is interposed. This molecular sieve membrane 6 allows the passage of salts having a small molecular size but DNA having a large molecular size.
It has physical properties that do not allow molecules to pass through. For example, a cellulose acetate film or a polyethersulfone film can be used.
【００１４】試料注入ウェル４の底部と分子ふるい膜６
との間の細管５の部分には、従来公知の分離泳動部ｂの
キャピラリー５０の先端が接続しており、該キャピラリ
ー５０の他方端は、第３のバッファー槽６０内に解放し
ている。さらに、キャピラリー５０の下方端近傍には蛍
光標識されたＤＮＡ断片等の被検出試料を検出する公知
の検出手段ｃが設けられている。The bottom of the sample injection well 4 and the molecular sieve film 6
And in the portion of the thin tube 5 between, and the tip of <br/> capillary -50 conventional known separation lane area b is connected, the capillary
The other end of the over 50 is released in the third buffer over bath 60. Further, the lower end near the capillary over 50 known detection means c for detecting the detected sample of DNA fragments or the like which is a fluorescent label is provided.
【００１５】第１、第２、第３のバッファー槽１、２、
６０には、それぞれ電極７１、７２、７３が設けられて
おり、各電極は電源切換スイッチ７４を介して泳動用高
圧電源７５に対し以下に記す２つの態様に選択的に切り
変わるように接続されている。すなわち、図１において
実線で示される第２のバッファー槽２に設けた泳動電極
７２と第１のバッファー槽１に設けた泳動電極７１との
間に電位を与える第１の切り換え態様と、点線で示され
る第１のバッファー槽１に設けた泳動電極７１と第３の
バッファー槽６０設けた泳動電極７３との間に電位を与
える第２の切り換え態様である。[0015] The first, second, third buffer over tank 1,
The electrode 60 is provided with electrodes 71, 72, and 73, respectively, and each electrode is connected to a high-voltage power supply for electrophoresis 75 via a power switch 74 so as to be selectively switched to the following two modes. ing. That is, a first switching mode in which a potential is applied between the migration electrode 72 provided in the second buffer tank 2 and the migration electrode 71 provided in the first buffer tank 1 shown by a solid line in FIG. This is a second switching mode in which a potential is applied between the migration electrode 71 provided in the first buffer tank 1 and the migration electrode 73 provided in the third buffer tank 60 as shown.
【００１６】次ぎに、この電気泳動装置を用いてＤＮＡ
断片の解析を行う方法を説明する。まず、すべてのバッ
ファー槽をバッファー液で満たす。しかる後、測定しよ
うとする蛍光標識されたＤＮＡ断片を含む試料をマイク
ロシリンジ等で１〜２μｌ採取し、第２バッファー槽２
の底部にある試料注入ウェル４に注入する。試料注入ウ
ェル４は第１バッファー槽１とキャピラリーの内径と同
程度の径の細管５で連結され、この細管５の途中すなわ
ち好ましくは第１バッファー槽１の底部近傍には、細管
５をふさぐように分子ふるい膜６が設けられ、かつ、試
料注入ウェル４の底部と分子ふるい膜６との間の細管５
の部分には、分離泳動部ｂのキャピラリー５０の先端が
接続している。従って、電源切換スウィッチ７４を図１
の実線で示す側にして第２のバッファー槽２に設けた泳
動電極７２と第１のバッファー槽２に設けた泳動電極７
１との間に電位を与えると、蛍光標識されたＤＮＡ断片
は第２のバッファー槽２下方の試料注入ウェル４から分
子ふるい膜６の方向に向かって泳動する。Next, a DNA is prepared using this electrophoresis apparatus.
A method for analyzing a fragment will be described. First, meet all buffers reactor with buffer over liquid. Thereafter, 1-2 μl of a sample containing the fluorescently labeled DNA fragment to be measured is collected with a microsyringe or the like, and the second buffer tank 2
Is injected into the sample injection well 4 at the bottom of the sample. The sample injection well 4 is connected to the first buffer tank 1 by a thin tube 5 having a diameter approximately the same as the inner diameter of the capillary, and the middle of the thin tube 5, that is, preferably near the bottom of the first buffer tank 1, is closed with the thin tube 5. Is provided with a molecular sieve film 6, and a capillary 5 between the bottom of the sample injection well 4 and the molecular sieve film 6.
The part, the tip end of the capillary over 50 separate lane area b is connected. Therefore, the power supply switching switch 74 is not shown in FIG.
The migration electrode 72 provided in the second buffer tank 2 and the migration electrode 7 provided in the first buffer tank 2 on the side indicated by the solid line.
When a potential is applied between the sample buffer and the sample buffer, the fluorescently labeled DNA fragment migrates from the sample injection well 4 below the second buffer tank 2 toward the molecular sieve membrane 6.
【００１７】バッファー液中の塩など分子サイズの小さ
な分子は分子ふるい膜６を自由に通過するが、蛍光標識
されたＤＮＡ断片分子は分子サイズが大きいため分子ふ
るい膜６の下部面あるいはその近傍の細管領域５'に濃
縮して集積し保持される。２〜５分間泳動させることに
より、ほぼすべての蛍光標識されたＤＮＡ断片分子を第
２のバッファー槽２から試料保持領域である前記の細管
領域５'へ濃縮して移すことができる。なお、この実施
例においては、分子ふるい膜６の下部の細管領域５'の
断面積は、泳動路断面すなわちキャピラリー１１の断面
積とほぼ同等となるようにしてあり、内径は０．１mm〜
０．０５mmが好適である。Although small molecules such as salts in the buffer solution pass freely through the molecular sieve membrane 6, the fluorescently labeled DNA fragment molecules have a large molecular size, so that the lower surface of the molecular sieve membrane 6 or the vicinity thereof can be used. It is concentrated and accumulated and held in the capillary region 5 '. By performing the electrophoresis for 2 to 5 minutes, almost all of the fluorescently labeled DNA fragment molecules can be concentrated and transferred from the second buffer tank 2 to the above-described capillary region 5 ′ which is a sample holding region. Incidentally, in this embodiment, the molecular cross-sectional area of the bottom of the capillary region 5 'of the old film 6 Yes set to be approximately equal to the cross-sectional area of the migration path sectional i.e. capillary chromatography 11 and an inner diameter 0.1mm~
0.05 mm is preferred.
【００１８】次いで、電源切換スウィッチ７４を図１に
点線で示す方へ倒し、第１のバッファー槽１に設けた泳
動電極７１と第３のバッファー槽６０に設けた泳動電極
７３との間に電位を与える。それにより、細管領域５'
に濃縮された状態で保持されている蛍光標識ＤＮＡ断片
は一斉に第３のバッファー槽６０の方向へ泳動を開始す
る。それにより蛍光標識されたＤＮＡ断片はゲルキャピ
ラリー５０内で分子サイズに応じて分離され、検出部ｃ
で検出される。[0018] Then, the power switching switch 74 in FIG. 1 Killed towards to indicate by dotted lines, between the first focusing electrode 73 that migration electrode 71 provided in the buffer tank 1 and provided with a third buffer tank 60 Apply potential. Thereby, the capillary region 5 ′
The fluorescently labeled DNA fragments held in a concentrated state start to migrate toward the third buffer tank 60 at the same time. Thereby, the fluorescently labeled DNA fragment is separated in the gel capillary 50 according to the molecular size,
Is detected by
【００１９】上記の説明から明らかなように、この電気
泳動装置においては、試料注入ウェルに注入された試料
がいったん濃縮された後、泳動分離部のキャピラリー内
に導入されるので、試料注入ウェル内の全試料を無駄な
く分離、検出の測定に使用することができ、計測に必要
な試料の量を極微量のものとすることができる。図２は
本発明による第２の実施例の試料注入部ａ2の断面を示
している。[0019] As apparent from the above description, in this electrophoresis apparatus, after the sample injected into the sample injection wells is once concentrated, since it is introduced into the capillary over the electrophoretic separation unit, sample injection wells All the samples can be used for measurement of separation and detection without waste, and the amount of sample required for measurement can be made extremely small. FIG. 2 shows a cross section of a sample injection part a2 of a second embodiment according to the present invention.
【００２０】この実施例の試料注入部ａ2は、第２のバ
ッファー槽２２の底部に設けた試料注入ウェルの底部に
第２の分子ふるい膜２６'を設けている点で第１の実施
例のものと基本的に異なっている。この実施例におい
て、第１及び第２のバッファー槽２１、２２の底部には
凹陥部が形成されており、該凹陥部の底部が細管２５に
より相互に連通されている。The sample injection section a 2 of this embodiment is different from the first embodiment in that a second molecular sieve film 26 ′ is provided at the bottom of a sample injection well provided at the bottom of the second buffer tank 22. Basically different from the ones. In this embodiment, the bottom portion of the first and second buffer over tank 21 is formed with a recess, the bottom of the recessed portion is communicated with each other by the capillary tube 25.
【００２１】第１のバッファー槽２１の底部に形成され
た凹陥部には細管２５の開口部を覆う状態で第１の実施
例のものと同様の分子ふるい膜２６が装着され、該分子
ふるい膜２６は貫通孔２７付き押さえ板２８により固定
されている。また、第２のバッファー槽２２の底部に形
成された凹陥部にも同様に細管２５の開口部を覆う状態
で第２の分子ふるい膜２６’が装着され、該分子ふるい
膜２６’は試料注入ウェル２４を兼ねた押さえ板２４’
により固定されている。第２の分子ふるい膜２６’は、
シーケンス解析に用いるＤＮＡ断片は透過し得るがそれ
より大きな分子サイズのものは透過しないメッシュをも
つ膜体が選択される。A molecular sieve film 26 similar to that of the first embodiment is attached to the recess formed at the bottom of the first buffer tank 21 so as to cover the opening of the thin tube 25. 26 is fixed by a holding plate 28 with a through hole 27. Similarly, a second molecular sieve film 26 'is attached to a concave portion formed at the bottom of the second buffer tank 22 so as to cover the opening of the thin tube 25, and the molecular sieve film 26' is used for sample injection. Holding plate 24 ′ that also serves as well 24
It is fixed by. The second molecular sieve film 26 ′
A membrane having a mesh that can transmit DNA fragments used for sequence analysis but does not transmit DNA fragments of a larger molecular size is selected.
【００２２】この実施例においても、特に図示しない
が、第３のバッファー槽、電源切換スウィッチ等電気泳
動による分析に必要な他の部材は第１の実施例のものと
同様に設けられる。この実施例を用いてのＤＮＡ断片の
解析も、第１の実施例の場合と同様に行われる。すなわ
ち、試料及びバッファー液をそれぞれ注入し、図１の実
線で示す側に切換スウィッチ７４を倒し泳動を開始す
る。この実施例においては、試料注入ウェル２４と細管
２５との間に上記のようなメッシュを持つ第２の分子ふ
るい膜２６'が設けられていることにより、ＤＮＡ相補
鎖合成反応を用いたＤＮＡシーケンス用断片生成に用い
るＭ１３ファージやプラスミドベクターなどのより大き
なサイズの鋳型ＤＮＡは第２の分子ふるい膜２６'を透
過せず、それよりも小さいサイズのもののみが透過す
る。従って、鋳型ＤＮＡと生成したＤＮＡ断片であるシ
ーケンス解析に用いる小さなＤＮＡ断片は第２の分子ふ
るい膜２６'により分離される。[0022] Also in this embodiment, although not particularly shown, a third buffer over bath, other members necessary for analysis by switching power switch and the like electrophoresis is provided in the same manner as in the first embodiment. Analysis of a DNA fragment using this embodiment is performed in the same manner as in the first embodiment. That is, sample and buffer over solution was poured respectively, to start electrophoresis defeat switching switch 74 to the side indicated by the solid line in FIG. 1. In this embodiment, since the second molecular sieve film 26 'having the mesh as described above is provided between the sample injection well 24 and the capillary 25, a DNA sequence using a DNA complementary strand synthesis reaction is provided. Larger size template DNA such as M13 phage or plasmid vector used for the production of the fragment does not permeate the second molecular sieve membrane 26 ', but only the smaller size DNA permeates. Therefore, the small DNA fragment used for sequence analysis, which is the template DNA and the generated DNA fragment, is separated by the second molecular sieve membrane 26 '.
【００２３】すなわち、鋳型ＤＮＡと生成したＤＮＡ断
片を含む溶液を試料注入ウェル２４に注入し、第２のバ
ッファー槽２２に設けた泳動電極７２と第１のバッファ
ー槽２１に設けた泳動電極７１との間に電位を与えるこ
とにより、分子サイズの小さい生成したＤＮＡ断片はバ
ッファー液中の塩などといっしょに第２の分子ふるい膜
２６’を通過し、第１のふるい膜２６の方向に泳動す
る。前記したように第２の分子ふるい膜２６’は分子サ
イズの大きな鋳型ＤＮＡを通さないように選定され、第
１のふるい膜２６は第１の実施例と同様にＤＮＡ断片を
通さないものが選定されているので、ＤＮＡ断片は第１
のふるい膜２６の下部面あるいはその近傍の試料保持領
域である細管領域２５’に濃縮して集積する。これによ
り試料保持領域に濃縮して集積するＤＮＡの総量中に占
めるシーケンス用ＤＮＡ断片の比率を第１の実施例のも
のと比較し、さらに上げることができる。以下の動作方
法は第１の実施例と同じである。That is, a solution containing the template DNA and the generated DNA fragment is injected into the sample injection well 24, and the electrophoresis electrode 72 provided in the second buffer tank 22 and the electrophoresis electrode 71 provided in the first buffer tank 21 By applying an electric potential between the two, the generated DNA fragment having a small molecular size passes through the second molecular sieve film 26 'together with the salt and the like in the buffer solution, and migrates in the direction of the first sieve film 26. . As described above, the second molecular sieve film 26 'is selected so as not to pass a template DNA having a large molecular size, and the first sieve film 26 is selected so as not to pass a DNA fragment as in the first embodiment. DNA fragment, the first
The sample is concentrated and accumulated in a capillary region 25 'which is a sample holding region at or near the lower surface of the sieve film 26. Thereby, the ratio of the sequencing DNA fragment in the total amount of DNA concentrated and accumulated in the sample holding region can be further increased as compared with that of the first embodiment. The following operation method is the same as in the first embodiment.
【００２４】本発明はキャピラリー型電気泳動装置ばか
りでなく分離泳動部に平板型ゲル（スラブゲル）を用い
た電気泳動装置にも適用できる。図３は本発明による第
３の実施例を示すスラブゲル用の試料注入部ａ3を表す
斜視図をその断面と共に示している。分離泳動部を構成
するゲル保持板５５、５５間に挟まれたスラブゲル５１
の上部にパッキング材５２を介して試料注入部ａ3が取
り付けられている。この試料注入部ａ3は、上記の実施
例と同様に二つのバッファー槽３１、３２とそれぞれの
二つの分子ふるい膜３６、３６'を有しているが、それ
らはゲル保持板５５のほぼ全長にわたって設けられてお
り、かつ第２のバッファー槽３２の底部にある試料注入
ウェル４４は、試料注入ウェル板４８内にゲル保持板５
５間に設けられる泳動路の数だけ存在し、かつ、二つの
バッファー槽の底部を連通する細管３５も同様に泳動路
の数だけ存在している。従って、泳動路の数に応じて試
料保持領域である細管領域３５'も存在することとな
る。また、この実施例においては泳動路の形状に応じ
て、該細管領域３５'の泳動路と直交する面での断面形
状を短冊型とすることもできる。The present invention can be applied not only to a capillary electrophoresis apparatus but also to an electrophoresis apparatus using a flat gel (slab gel) in a separation / phoresis section. Figure 3 shows a perspective view showing the sample injection unit a 3 for slab gel showing a third embodiment according to the present invention together with the cross-section. Slab gel 51 sandwiched between gel holding plates 55 constituting a separation / phoresis section
Sample injection unit a 3 through a packing material 52 of the upper are attached. This sample injection part a 3 has two buffer tanks 31 and 32 and two molecular sieve films 36 and 36 ′, respectively, as in the above embodiment. provided over, and sample injection well 44 at the bottom of the second bus Tsu fur tank 32, gel holding plate 5 to the sample injection wells plate 48
There is only the number of the migration path provided between 5 and are present as many as the number of capillaries 35 likewise migration path for communicating the bottom of the two <br/> buffer over bath. Therefore, a capillary region 35 'which is a sample holding region also exists according to the number of migration paths. Further, in this embodiment, the cross-sectional shape of the narrow tube region 35 'on a surface orthogonal to the migration path can be formed in a rectangular shape according to the shape of the migration path.
【００２５】スラブゲル形式の泳動装置においては、泳
動路の断面積（あるいは幅）は試料の保持される部分の
幅と泳動中に拡散で広がる幅で決定される。従って、試
料の拡散による広がりを防止するために、泳動路の中に
高分子リボンあるいはワイヤーを設置し、隣接する泳動
路を独立させるようにしてもよい。試料の濃縮の方法、
その他の動作方法は第１、第２の実施例と同じであるの
で、説明は省略する。図３では泳動電極は記載していな
い。In a slab gel type electrophoresis apparatus, the cross-sectional area (or width) of an electrophoresis path is determined by the width of a portion where a sample is held and the width spread by diffusion during electrophoresis. Therefore, in order to prevent the sample from spreading due to diffusion, a polymer ribbon or a wire may be provided in the migration path, and the adjacent migration path may be made independent. How to concentrate the sample,
Other operation methods are the same as those of the first and second embodiments, and the description is omitted. In FIG. 3, the electrophoresis electrode is not shown.
【００２６】図４は本発明による第４の実施例を示す試
料注入部ａ4を表す断面図である。この実施例のもの
は、第１と第２の二つのバッファー槽を上下方向に一方
が他方の内部に入り込む形で設置した点で上記の他の実
施例のものと異なっている。すなわち、外側のバッファ
ー槽４２内にもう一つのバッファー槽４１が設けられて
おり、それぞれ第１から第３の実施例における第２のバ
ッファー槽及び第１のバッファー槽に対応した機能を果
たすように構成されている。バッファー槽４１の底部に
は他の実施例と同様に分離泳動部ｂのキャピラリー４１
の先端が接続しており、該キャピラリー４１は外側のバ
ッファー槽４２の底部を貫通して、図示しない第３のバ
ッファー槽に達している。バッファー槽４１の底部には
同様に分子ふるい膜４６が設けられ貫通孔付き押さえ板
４８により固定されている。FIG. 4 is a sectional view showing a sample injection part a4 according to a fourth embodiment of the present invention. This embodiment is different from the above-mentioned other embodiments in that the first and second buffer tanks are installed in such a manner that one of them enters the other in the vertical direction. That is, the outer buffer
Another is Buffer over bath 41 is provided, functions corresponding from the first, respectively third second bar <br/> Ffa chromatography tank and a first buffer over tank in the embodiment of the chromatography tank 42 It is configured to fulfill. Capillary chromatography of the bottom of the buffer over bath 41 another embodiment similarly to the separation electrophoresis unit b 41
Has distal end of the connection, said capillary chromatography 41 through the bottom of the outer bar <br/> Ffa over tank 42 has reached the third bar <br/> Ffa over tank (not shown). It is fixed by bottom Similarly molecular sieve membrane 46 provided at the portion through the perforated presser plate 48 of the buffer over bath 41.
【００２７】この実施例においては、キャピラリー４１
には外側のバッファー槽４２内に位置している個所にお
いて細孔が設けるかあるいは切開した細隙４５が形成さ
れる。その細孔あるいは細隙４５を介して、バッファー
槽４２内の空間とキャピラリーの空間とが連通してい
る。また、特に図示しないが、各泳動電極が上記の他の
実施例と同様に各バッファー槽に装着されている。[0027] In this embodiment, capillary chromatography 41
Or incised slit 45 pores at points located outside the buffer over bath 42 provided is formed in the. Through the pores or interstices 45, and the space of the spatial and capillary chromatography in the buffer over <br/> tank 42 are communicated. Further, although not particularly shown, each electrophoretic electrode is attached to each buffer over tanks as well as other embodiments described above.
【００２８】使用に際し、外側の第２のバッファー槽４
２に試料を注入する。この試料が注入されるバッファー
槽４２の底部が試料注入ウェルに相当する。各バッファ
ー槽にバッファー液を満たした後他の実施例と同様にし
て泳動を行う。この実施例にあってもキャピラリーに設
けられた細孔あるいはキャピラリーの切開した細隙部４
５から電気泳動によりＤＮＡ断片が進入し、分子ふるい
膜４６の直下である試料保持領域４５'に試料が濃縮さ
れて導かれることが容易に理解されよう。その後の動作
方法は第１の実施例と同じである。In use, the outer second buffer tank 4
2. Inject the sample into 2. The bottom of the buffer tank 42 into which the sample is injected corresponds to a sample injection well. Each buffer
Performing electrophoresis in the same manner as other examples after filling a buffer over liquid chromatography tank. Also in this embodiment, the pores provided in the capillary or the slit portion 4 cut out of the capillary are provided.
From FIG. 5, it can be easily understood that the DNA fragment enters by electrophoresis and the sample is concentrated and led to the sample holding region 45 ′ immediately below the molecular sieve membrane 46. The subsequent operation method is the same as in the first embodiment.
【００２９】この実施例において、キャピラリーに設け
られた細孔あるいはキャピラリーの切開した細隙４５よ
り上方、即ち内方の第１のバッファー槽４１に連結する
キャピラリー内には必ずしもゲルが形成されていなくと
もよい。また、切断部を設ける場合には第１のバッファ
ー槽４１は外側の第２のバッファー槽４２との間に一定
の位置関係を保ち保持される必要がある。In this embodiment, the gel is not necessarily formed above the pores provided in the capillary or above the slit 45 formed in the capillary, that is, in the capillary connected to the first buffer tank 41 inside. May be. In the case where a cutting portion is provided, the first buffer tank 41 needs to be held in a fixed positional relationship with the outer second buffer tank 42.
【００３０】特に図示しないが、この実施例のものを図
３に示した実施例のような平板型ゲルを用いた装置にも
適用できることは容易に理解されよう。なお、以上の第
１〜第４の実施例では分離部にゲルを持つ場合について
述べたが、ゲルなどの担体を持たない場合にも本発明は
適用できる。Although not particularly shown, it will be easily understood that this embodiment can be applied to an apparatus using a flat gel like the embodiment shown in FIG. In the above-described first to fourth embodiments, the case where the separation portion has a gel has been described. However, the present invention can be applied to a case where a carrier such as a gel is not provided.
【００３１】[0031]
【発明の効果】本発明によれば、広い試料注入ウェルに
注入した試料を電気泳動により、狭い領域に濃縮して保
持した後に、電気泳動分離するので作製した試料の大部
分を有効に分析に使用でき、濃度の低い試料でも感度良
く分離、検出することができる。According to the present invention, a sample injected into a wide sample injection well is concentrated by electrophoresis in a narrow area and held, and then subjected to electrophoretic separation, so that most of the prepared sample can be effectively analyzed. It can be used and can be separated and detected with high sensitivity even for low concentration samples.
【図１】本発明による第１の実施例の概念図。FIG. 1 is a conceptual diagram of a first embodiment according to the present invention.
【図２】本発明による第２の実施例の断面図。FIG. 2 is a sectional view of a second embodiment according to the present invention.
【図３】本発明による平板ゲルを用いた第３の実施例の
断面による見取図。FIG. 3 is a cross-sectional view of a third embodiment using a flat gel according to the present invention.
【図４】本発明による第４の実施例の断面図。FIG. 4 is a sectional view of a fourth embodiment according to the present invention.
【図５】従来のキャピラリー電気泳動装置の概念図。FIG. 5 is a conceptual diagram of a conventional capillary electrophoresis apparatus.
１…第１バッファー槽、２…第２バッファー槽、６０…
第３のバッファー槽、４…試料注入ウェル、５…細管、
５’…細管領域（試料保持部領域）、６…第１の分子ふ
るい膜、２６’…第２の分子ふるい膜、７１，７２，７
３…泳動電極、７４…泳動電源切換スウィッチ、７５…
泳動電源、ａ…試料注入部、ｂ…泳動分離部、ｃ…検出
部DESCRIPTION OF SYMBOLS 1 ... 1st buffer tank, 2 ... 2nd buffer tank, 60 ...
Third buffer tank, 4 ... sample injection well, 5 ... capillary,
5 ′: capillary region (sample holding region), 6: first molecular sieve film, 26 ′: second molecular sieve film, 71, 72, 7
3: electrophoresis electrode, 74: electrophoretic power supply switching switch, 75:
Electrophoresis power supply, a: sample injection section, b: electrophoresis separation section, c: detection section
フロントページの続き (56)参考文献 特開 昭63−231247（ＪＰ，Ａ) 特開 平２−269935（ＪＰ，Ａ) 特開 昭61−13148（ＪＰ，Ａ) 特開 平４−127049（ＪＰ，Ａ) 特開 平２−165047（ＪＰ，Ａ) 特開 平１−147355（ＪＰ，Ａ) 特開 平３−68860（ＪＰ，Ａ) 特開 昭63−262554（ＪＰ，Ａ) 特開 平３−223667（ＪＰ，Ａ) 特開 平３−87648（ＪＰ，Ａ) 特開 昭50−16677（ＪＰ，Ａ) 特開 昭63−191051（ＪＰ，Ａ) 特開 平５−215716（ＪＰ，Ａ) 特表 平４−502516（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 27/447 ＷＰＩ（ＤＩＡＬＯＧ)Continuation of the front page (56) References JP-A-63-231247 (JP, A) JP-A-2-269935 (JP, A) JP-A-61-13148 (JP, A) JP-A-4-127049 (JP, A) JP-A-2-165047 (JP, A) JP-A-1-147355 (JP, A) JP-A-3-68860 (JP, A) JP-A-63-262554 (JP, A) JP-A-3-223667 (JP, A) JP-A-3-87648 (JP, A) JP-A-50-16677 (JP, A) JP-A-63-191051 (JP, A) JP-A-5-215716 (JP, A A) Tokuhei 4-502516 (JP, A) (58) Fields surveyed (Int. Cl. 7 , DB name) G01N 27/447 WPI (DIALOG)
Claims (10)
泳動用高圧電源供給部とからなる蛍光検出型電気泳動装
置において、該試料注入部は相互に細管部分で連結され
た二つのバッファー槽及び該細管部分に位置する検出対
象物は通過し得ないが検出対象物より小さいサイズの物
質は通過し得る第１の分子ふるい膜とを有していると共
に、該分離泳動部は該分子ふるい膜の近傍上流側に開口
しており、かつ泳動用高圧電源供給部は該二つのバッフ
ァー槽間及び二つのバッファー槽間の一方と分離泳動部
間とに切り替え自在に接続していることを特徴とする電
気泳動装置。In a fluorescence detection type electrophoresis apparatus comprising a sample injection section, a separation and migration section, a detection section, and a high voltage power supply section for electrophoresis, the sample injection section is connected to two buffers connected to each other by a thin tube portion. A first molecular sieve membrane through which the detection object located in the vessel and the capillary portion cannot pass but a substance smaller in size than the detection object can pass; switching molecule is opened near the upstream side of the sieve film, and high-voltage power supply unit for electrophoresis and between one and the separation lane area between between the two buffer <br/> § chromatography tank and two buffers over bath An electrophoresis apparatus characterized by being freely connected.
は通過し得うるが検出対象物より大きいサイズの物質の
通過は阻止する第２の分子ふるい膜が設けられており、
該分離泳動部は、第２と第１の分子ふるい膜間の該細管
部分に開口していることを特徴とする、請求項１記載の
装置。2. A second molecular sieve membrane is provided on the upstream side of the capillary portion so as to allow a detection target to pass therethrough, but to block passage of a substance having a size larger than the detection target,
2. The apparatus according to claim 1, wherein the separation electrophoresis section is open to the capillary portion between the second and first molecular sieve membranes.
空キャピラリーであることを特徴とする、請求項１また
は２記載の装置。3., wherein the separation lane area is hollow calibration pin rally filled with buffer over liquid, according to claim 1 or 2 wherein.
形成されたゲルであることを特徴とする、請求項１ない
し３いずれか記載の装置。4., wherein the separation lane area is a gel formed inside Garasukya pin rally, device according to any one of claims 1 to 3.
成された平板型ゲルであることを特徴とする、請求項１
または２記載の装置。5. The method according to claim 1, wherein the separation electrophoresis section is a flat gel formed between two glass flat plates.
Or the apparatus according to 2.
積とほぼ等しいかあるいはより小さな面積に制限する手
段を具備することを特徴とする、請求項１ないし５いず
れか記載の装置。6. The apparatus according to claim 1, further comprising means for limiting an area of the molecular sieve membrane to an area substantially equal to or smaller than a cross-sectional area of the separation / phoresis section.
１、第２のバッファー槽を連結する細管と、一方の端部
が前記細管の途中に開口し、検出対象物を電気泳動分離
する分離泳動部と、前記一方の端部と前記第１のバッフ
ァー槽との間の前記細管の途中に配置され、前記検出対
象物を通過させず前記検出対象物より小さいサイズの物
質を通過させる分子ふるい膜と、前記第１、第２のバッ
ファー槽内にそれぞれ配置される第１、第２の電極とを
具備し、前記第１の電極と前記第２の電極との間に電圧
が印加され、前記第２のバッファー槽に注入された前記
検出対象物を含む試料が濃縮されて、前記分子ふるい膜
と前記一方の端部との間の前記細管の部分に保持される
ことを特徴とする電気泳動装置。7. A first and second buffer tank, a thin tube connecting the first and second buffer tanks, one end of which is opened in the middle of the thin tube, and an object to be detected is electrophoretically separated. Separation electrophoresis section, which is disposed in the middle of the thin tube between the one end and the first buffer tank, and allows a substance having a size smaller than the detection target to pass without passing the detection target. A molecular sieve membrane, and first and second electrodes respectively disposed in the first and second buffer tanks, wherein a voltage is applied between the first electrode and the second electrode. The sample containing the detection object injected into the second buffer tank is concentrated, and is held in a portion of the capillary tube between the molecular sieve membrane and the one end. Electrophoresis device.
１、第２のバッファー槽を連結する細管と、一方の端部
が前記細管の途中に開口し、検出対象物を電気泳動分離
する分離泳動部と、前記細管の前記第１のバッファー槽
の末端側に配置され、前記検出対象物を通過させず前記
検出対象物より小さいサイズの物質を通過させる第１の
分子ふるい膜と、前記細管の前記第２のバッファー槽の
末端側に配置され、前記検出対象物を通過させ前記検出
対象物より大きいサイズの物質の通過を阻止する第２の
分子ふるい膜と、前記第１、第２のバッファー槽内にそ
れぞれ配置される第１、第２の電極とを具備し、前記第
１の電極と前記第２の電極との間に電圧が印加され、前
記第２のバッファー槽に注入された前記検出対象物を含
む試料が濃縮されて、前記第１の分子ふるい膜と前記一
方の端部との間の前記細管の部分に保持されることを特
徴とする電気泳動装置。8. A first and second buffer tank, a thin tube connecting the first and second buffer tanks, one end of which is opened in the middle of the thin tube, and an object to be detected is electrophoretically separated. A separation and migration section, and a first molecular sieve membrane that is disposed on the terminal side of the first buffer tank of the thin tube and allows a substance smaller in size than the detection target to pass without passing the detection target; A second molecular sieve membrane that is disposed on the terminal side of the second buffer tank of the thin tube and that allows the detection target to pass therethrough and blocks the passage of a substance having a size larger than the detection target; And a first electrode and a second electrode disposed in the second buffer tank, respectively. A voltage is applied between the first electrode and the second electrode, and the voltage is applied to the second buffer tank. The sample containing the detection target is concentrated, An electrophoresis apparatus, wherein the electrophoresis apparatus is held in a portion of the capillary tube between the first molecular sieve membrane and the one end.
のバッファー槽の底部に配置される複数の試料注入ウェ
ルと、前記第１のバッファー槽と前記各試料注入ウェル
とを連結する複数の細管と、一方の端部が前記複数の細
管の各々の途中に開口し、検出対象物を電気泳動分離す
る複数の電気泳動路を有する分離泳動部と、前記複数の
細管の前記第１のバッファー槽の側の末端に配置され、
前記検出対象物を通過させず前記検出対象物より小さい
サイズの物質を通過させる第１の分子ふるい膜と、前記
複数の細管の前記第２のバッファー槽の側の末端と前記
各試料注入ウェルとの間に配置され、前記検出対象物を
通過させ前記検出対象物より大きいサイズの物質の通過
を阻止する第２の分子ふるい膜と、前記第１、第２のバ
ッファー槽内にそれぞれ配置される第１、第２の電極と
を具備し、前記第１の電極と前記第２の電極との間に電
圧が印加され、前記各試料注入ウェルに注入された前記
検出対象物を含む試料が濃縮されて、前記第１の分子ふ
るい膜と前記複数の電気泳動路の一方の端部との間に前
記複数の細管の部分に保持されることを特徴とする電気
泳動装置。9. The first and second buffer tanks and the second buffer tank.
A plurality of sample injection wells arranged at the bottom of the buffer tank, a plurality of thin tubes connecting the first buffer tank and each of the sample injection wells, and one end portion is in the middle of each of the plurality of thin tubes. The separation electrophoresis section having a plurality of electrophoresis paths for electrophoretically separating the detection target, and disposed at the end of the plurality of thin tubes on the side of the first buffer tank,
A first molecular sieve membrane that allows passage of a substance smaller in size than the detection target without passing through the detection target, an end of the plurality of capillaries on the side of the second buffer tank, and each of the sample injection wells; And a second molecular sieve membrane that passes through the detection target and blocks a substance having a size larger than the detection target, and is disposed in the first and second buffer tanks, respectively. A first electrode, a second electrode, a voltage is applied between the first electrode and the second electrode, and the sample containing the detection target injected into each of the sample injection wells is concentrated. The electrophoresis apparatus is characterized in that the electrophoresis apparatus is held between the first molecular sieve membrane and one end of the plurality of electrophoresis paths by the plurality of capillary tubes.
ッファー槽の内部に配置される第１のバッファー槽と、
一方の端部が前記第１のバッファー槽の底部に接続し、
前記第２のバッファー槽に連通する細孔を有し、前記第
２のバッファー槽の底部を貫通する電気泳動部であり、
検出対象物を電気泳動分離する分離泳動部と、前記分離
泳動部の前記一方の端部に配置され、前記検出対象物を
通過させず前記検出対象物より小さいサイズの物質を通
過させる分子ふるい膜と、前記第１、第２のバッファー
槽内にそれぞれ配置される第１、第２の電極とを具備
し、前記第１の電極と前記第２の電極との間に電圧が印
加され、前記第２のバッファー槽に注入された前記検出
対象物を含む試料が濃縮されて、前記分子ふるい膜と前
記細孔が形成された部位との間の前記電気泳動路の部分
に保持されることを特徴とする電気泳動装置。10. A second buffer tank, a first buffer tank disposed inside the second buffer tank,
One end is connected to the bottom of the first buffer tank,
An electrophoresis section having pores communicating with the second buffer tank and penetrating the bottom of the second buffer tank;
A separation electrophoresis section for electrophoretically separating an object to be detected, and a molecular sieve membrane disposed at the one end of the separation electrophoresis section and passing a substance smaller in size than the object to be detected without passing through the object to be detected And a first and a second electrode respectively disposed in the first and second buffer tanks, wherein a voltage is applied between the first and the second electrodes, The sample containing the object to be detected injected into the second buffer tank is concentrated and held in a portion of the electrophoresis path between the molecular sieve membrane and the site where the pores are formed. Characteristic electrophoresis device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP03233489A JP3075602B2 (en) | 1991-09-12 | 1991-09-12 | Electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP03233489A JP3075602B2 (en) | 1991-09-12 | 1991-09-12 | Electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0572178A JPH0572178A (en) | 1993-03-23 |
| JP3075602B2 true JP3075602B2 (en) | 2000-08-14 |
Family
ID=16955817
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP03233489A Expired - Fee Related JP3075602B2 (en) | 1991-09-12 | 1991-09-12 | Electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3075602B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8361716B2 (en) | 2008-10-03 | 2013-01-29 | Pathogenetix, Inc. | Focusing chamber |
| US8685708B2 (en) | 2012-04-18 | 2014-04-01 | Pathogenetix, Inc. | Device for preparing a sample |
| US8999636B2 (en) | 2007-01-08 | 2015-04-07 | Toxic Report Llc | Reaction chamber |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9509905D0 (en) * | 1995-05-11 | 1995-07-12 | Watson Arthur H | Fast sample device for capillary isoelectric focusing |
| EP0999443A3 (en) | 1998-11-02 | 2002-09-18 | The Institute of Physical and Chemical Research | Capillary electrophoretic apparatus, sample plate and sample injection method |
| US6372106B1 (en) * | 1999-07-26 | 2002-04-16 | Applera Corporation | Capillary electrophoresis method and apparatus for reducing peak broadening associated with the establishment of an electric field |
| US6770182B1 (en) * | 2000-11-14 | 2004-08-03 | Sandia National Laboratories | Method for producing a thin sample band in a microchannel device |
| US8029743B2 (en) * | 2007-09-19 | 2011-10-04 | General Electric Company | Microfluidic device with vertical injection aperture |
| JP2017078571A (en) * | 2014-03-07 | 2017-04-27 | テルモ株式会社 | Gel electrophoresis device and gel electrophoresis method |
-
1991
- 1991-09-12 JP JP03233489A patent/JP3075602B2/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8999636B2 (en) | 2007-01-08 | 2015-04-07 | Toxic Report Llc | Reaction chamber |
| US8361716B2 (en) | 2008-10-03 | 2013-01-29 | Pathogenetix, Inc. | Focusing chamber |
| US8685708B2 (en) | 2012-04-18 | 2014-04-01 | Pathogenetix, Inc. | Device for preparing a sample |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0572178A (en) | 1993-03-23 |
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