JP2841556B2 - Electrophoresis device - Google Patents
Electrophoresis deviceInfo
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- JP2841556B2 JP2841556B2 JP1256460A JP25646089A JP2841556B2 JP 2841556 B2 JP2841556 B2 JP 2841556B2 JP 1256460 A JP1256460 A JP 1256460A JP 25646089 A JP25646089 A JP 25646089A JP 2841556 B2 JP2841556 B2 JP 2841556B2
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- Prior art keywords
- electrophoresis
- sub
- liquid tank
- path
- electrode liquid
- Prior art date
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Description
【発明の詳細な説明】 （イ）産業上の利用分野 この発明は電気泳動装置に関する。さらに詳しくは、
希薄試料の高度の分離等に好適な細管式電気泳動装置に
関する。The present invention relates to an electrophoresis apparatus. For more information,
The present invention relates to a capillary electrophoresis apparatus suitable for high-grade separation of a dilute sample.
（ロ）従来の技術及び課題 キャピラリ電気泳動管を用いる細管式電気泳動におい
てキャピラリゾーン泳動は、理論段数にして数10万段に
達する高分離能を有するが、試料注入量は数10μが限
界でこれを越える量を注入すると分離能が低下するた
め、希薄溶液には適用できなかった。(B) Conventional technology and problems In capillary electrophoresis using capillary electrophoresis tubes, capillary zone electrophoresis has high resolution, reaching several hundred thousand theoretical plates, but the sample injection amount is limited to several tens of microns. Injecting more than this amount would reduce the resolution, and could not be applied to dilute solutions.
一方等速電気泳動は希薄試料を濃縮することができる
が、分離能力が充分ではない。On the other hand, isotachophoresis can concentrate a dilute sample, but does not have sufficient separation ability.
この発明はかかる状況に鑑み為されたものであり、希
薄試料を高分離能のもとに泳動分離できる電気泳動装置
を提供しようとするものである。The present invention has been made in view of such a situation, and an object of the present invention is to provide an electrophoresis apparatus capable of performing electrophoretic separation of a dilute sample with high resolution.
（ハ）課題を解決するための手段 かくしてこの発明によれば、（１）ターミナル電極液
槽とリーディング電極液槽との間を、試料注入部を介し
て接続されるキャピラリ電気泳動管で構成される主電気
泳動路と、（２）上記キャピラリ電気泳動管の試料注入
部の後段に別々に分岐接続され、かつ各々副リーディン
グ電極液槽に接続されたキャピラリ電気泳動管からな
る、先に分岐された第１の副電気泳動路及び後で分岐さ
れた第２の副電気泳動路と、（３）上記主電気泳動路の
各分岐接続部の前段及び上記第２の副電気泳動路にそれ
ぞれ設けられる検出手段と、（４）上記第１の副電気泳
動路の分岐接続部の前段に設けられた検出手段により分
析対象の成分ゾーンを検出するまでターミナル電極液槽
と第１の副リーディング電極液槽との間に、次いで第２
の副電気泳動路の前段に設けられた検出手段により分析
対象の成分ゾーンの通過を検出するまでターミナル電極
液槽とリーディング電極液槽との間に、その後はリーデ
ィング電極液槽と第２の副電気泳動路との間に、それぞ
れ所定の電圧を印加する電圧印加手段とを備えてなる電
気泳動装置が提供される。(C) Means for Solving the Problems According to the present invention, (1) a capillary electrophoresis tube connected between a terminal electrode liquid tank and a leading electrode liquid tank via a sample injection section is provided. And (2) a capillary electrophoresis tube, which is separately branched after the sample injection section of the capillary electrophoresis tube and connected to the sub-leading electrode solution tank, and is branched first. The first sub-electrophoresis path, the second sub-electrophoresis path branched later, and (3) the first sub-electrophoresis path and the second sub-electrophoresis path provided before and after the respective branch connection portions of the main electrophoresis path. (4) a terminal electrode solution tank and a first sub-leading electrode solution until a component zone to be analyzed is detected by a detection device provided before the branch connection of the first sub-electrophoresis path. Between the tank Then the second
Between the terminal electrode liquid tank and the leading electrode liquid tank until the passage of the component zone to be analyzed is detected by the detection means provided in the preceding stage of the sub-electrophoresis path, and thereafter, the leading electrode liquid tank and the second sub-electrode liquid tank. There is provided an electrophoresis apparatus including a voltage application unit for applying a predetermined voltage between the electrophoresis path and the electrophoresis path.
この発明の装置において、主電気泳動路には複数の副
電気泳動路が分岐接続される。上記主電気泳動路及び副
電気泳動路にはいずれも当該分野で公知のキャピラリ電
気泳動管が用いられる。In the apparatus of the present invention, a plurality of sub-electrophoresis paths are branched and connected to the main electrophoresis path. For each of the main electrophoresis path and the sub-electrophoresis path, a capillary electrophoresis tube known in the art is used.
上記複数の副電気泳動路はそれらの各端部がそれぞれ
電極液槽に接続される。各電極液槽には電極及びリーデ
ィング電極液が備えられる。上記各電極液槽は独立して
構成されていることが好ましい。Each end of the plurality of sub-electrophoresis paths is connected to an electrode liquid tank. Each electrode solution tank is provided with an electrode and a leading electrode solution. It is preferable that each of the electrode liquid tanks is configured independently.
上記複数の副電気泳動路は、少なくとも、主電気泳動
路に注入された試料について該泳動路で等速電気泳動に
より分離される成分のうちから、分析対象外のものを泳
動誘導して除去するためのいわゆるトラップ用泳動路
と、上記分離成分のうち分析対象の成分をゾーン泳動し
うるためのゾーン泳動路との２つの泳動路、つまり主電
気泳動路から先に分岐された第１の副電気泳動路と、後
で分岐された第２の副電気泳動路とからなる。The plurality of sub-electrophoresis paths at least remove, by electrophoretically inducing, out of components separated by isotachophoresis in the migration path the sample injected into the main electrophoresis path, those not analyzed. A migration path for trapping, and a zone migration path for zone-migrating the component to be analyzed among the separated components, that is, a first auxiliary path branched first from the main electrophoresis path. It is composed of an electrophoresis path and a second sub-electrophoresis path branched later.
上記主電気泳動路に対する副電気泳動路の分岐接続
は、当該分野で公知の方法によりなされる。The branch connection of the sub electrophoresis path to the main electrophoresis path is performed by a method known in the art.
この発明の装置において、上記主電気泳動路への副電
気泳動路の分岐接続部前段及び第２の副電気泳動路には
それぞれ検出手段が設けられる。分岐接続部前段とは、
泳動方向にみて分岐接続部手前を意味しこれはこの分岐
接続部にできるだけ近い部分が好ましい。上記検出手段
としては、電導度検出器、UV検出や蛍光検出できる光学
検出器等が挙げられる。また、泳動に要した電気量から
泳動距離を割り出すものも検出器として使用できる。In the apparatus according to the present invention, a detecting means is provided in each of a stage before the branch connection portion of the sub-electrophoresis path to the main electrophoresis path and a second sub-electrophoresis path. What is the former stage of the branch connection section?
In the electrophoresis direction, it means just before the branch connection, which is preferably as close as possible to the branch connection. Examples of the detection means include a conductivity detector, an optical detector capable of UV detection and fluorescence detection, and the like. A detector that determines the migration distance from the amount of electricity required for migration can also be used as a detector.
この発明の装置において、電圧印加手段としては、タ
ーミナル電極液槽とリーディング電極液槽もしくは副リ
ーディング電極液槽との間、並びにリーディング電極液
槽といずれかの副リーディング電極液槽との間に、所定
の電圧を切換え可能に印加できるよう構成されたものが
用いられる。すなわち、上記電圧印加手段は、易動度の
小さいイオンを含有する電極液（すなわちターミナル電
極液）と易動度の大きいイオンを含有する電極液（すな
わちリーディング電極液）との間に試料を挟んで主電気
泳動路及び第１の副電気泳動路で等速電気泳動ができる
と共に、易動度の大きい電極液間に特定の試料を挟んで
第２の副電気泳動路でゾーン泳動できるように構成され
る。この構成の一例としては定電流電源と該電源に切換
え接続できる等速電気泳動用回路及びゾーン泳動用回路
とからなるものが挙げられる。In the apparatus of the present invention, as the voltage applying means, between the terminal electrode liquid tank and the leading electrode liquid tank or the sub-leading electrode liquid tank, and between the leading electrode liquid tank and any of the sub-leading electrode liquid tanks, One configured to be able to switchably apply a predetermined voltage is used. That is, the voltage applying means sandwiches the sample between the electrode solution containing ions having low mobility (that is, the terminal electrode solution) and the electrode solution containing ions having high mobility (that is, the leading electrode solution). In this way, isotachophoresis can be performed in the main electrophoresis path and the first sub-electrophoresis path, and zone electrophoresis can be performed in the second sub-electrophoresis path with a specific sample interposed between electrode solutions having high mobility. Be composed. An example of this configuration includes a constant current power supply and a circuit for constant velocity electrophoresis and a circuit for zone migration that can be switched to and connected to the power supply.
（ニ）作用 この発明によれば、試料注入部に注入された試料は、
ターミナル電極液槽とリーディング電極液槽もしくは副
リーディング電極液槽との間に印加された電圧により、
主電気泳動路を等速電気泳動されて試料は各成分ゾーン
に分離され、分析対象の成分が１ゾーンに濃縮されるこ
ととなる。(D) Function According to the present invention, the sample injected into the sample injection section is
By the voltage applied between the terminal electrode liquid tank and the leading electrode liquid tank or the sub-leading electrode liquid tank,
The sample is separated at each component zone by the uniform electrophoresis in the main electrophoresis path, and the component to be analyzed is concentrated in one zone.
次いで、分析対象の成分ゾーンが検出されるまでその
先行する不要な部分は第１の副電気泳動路に泳動され
て、除去されることとなる。Next, the preceding unnecessary portion is migrated to the first sub-electrophoresis path and removed until the component zone to be analyzed is detected.
さらに、試料はその分析対象の成分ゾーンを先頭とし
て主電気泳動路を等速電気泳動され、第２の副電気泳動
路の分岐部より後段の主電気泳動路に導入される。Further, the sample is subjected to uniform velocity electrophoresis in the main electrophoresis path with the component zone to be analyzed at the head, and introduced into the main electrophoresis path subsequent to the branch of the second sub-electrophoresis path.
その後リーディング電極液槽と第２の副電気泳動路の
副リーディング電極液槽との間に所定の電圧が印加さ
れ、上記成分ゾーンが該副電気泳動路でキャピラリゾー
ン泳動されることとなる。Thereafter, a predetermined voltage is applied between the leading electrode liquid tank and the sub-leading electrode liquid tank of the second sub-electrophoresis path, and the component zone is subjected to capillary zone migration in the sub-electrophoresis path.
以下実施例によりこの発明を詳細に説明するが、これ
によりこの発明は限定されるものではない。Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited thereto.
（ホ）実施例 第１図はこの発明の電気泳動装置の一例の構成説明図
である。この図において電気泳動装置（１）は、ターミ
ナル電極液槽（２）と、リーディング電極液槽（３）
と、これらの間を試料注入部（４）を介して接続するキ
ャピラリ電気泳動管で構成される主電気泳動路（５）
と、この主電気泳動路（５）の試料導入部の後段に別々
に分岐接続されるキャピラリ電気泳動管からなる、先に
分岐された第１の副電気泳動路（６）及び後で分岐され
た第２の副電気泳動路（７）と、これら第１・２の副電
気泳動路（６）（７）のそれぞれ端部に設けられる副リ
ーディング電極液槽（８）（９）と、第１・第２の副電
気泳動（６）（７）の分岐接続部（イ）（ロ）の各前段
に設定される検出器（10）（11）と、第２の副電気泳動
路（７）に設けられる検出器（12）と、ターミナル電極
液槽（２）、リーディング電極液槽（３）及び副リーデ
ィング電極液槽（８）（９）のそれぞれに順に設けられ
る電極（21）（31）（81）（91）と、図示しない定電流
電源及び図示しない回路切換手段とから主として構成さ
れている。(E) Embodiment FIG. 1 is an explanatory view of the configuration of an example of the electrophoresis apparatus of the present invention. In this figure, an electrophoresis apparatus (1) includes a terminal electrode liquid tank (2) and a leading electrode liquid tank (3).
And a main electrophoresis path (5) composed of a capillary electrophoresis tube connecting these via a sample injection part (4).
A first sub-electrophoresis path (6) branched first and a capillary electrophoresis tube separately branched after the sample introduction section of the main electrophoresis path (5) A second sub-electrophoresis path (7), sub-leading electrode tanks (8) and (9) provided at respective ends of the first and second sub-electrophoresis paths (6) and (7), Detectors (10) and (11) set before each of the branch connection portions (a) and (b) of the first and second sub-electrophoresis (6) and (7), and the second sub-electrophoresis path (7) ) And the electrodes (21) (31) provided in the terminal electrode liquid tank (2), the leading electrode liquid tank (3), and the sub-leading electrode liquid tanks (8) and (9), respectively. ) (81) and (91), and a constant current power supply (not shown) and circuit switching means (not shown).
上記主電気泳動路（５）を構成するキャピラリ電気泳
動管は、内径が200〜500μｍでかつ試料注入部（４）か
らリーディング電極液槽（３）までの長さが0.5mに設定
されている。２つの副電気泳動路（６）（７）のうち、
（６）は内径200〜500μｍ、長さ0.2m、（７）は内径50
〜100μm,長さ0.5mにそれぞれ設定されている。The capillary electrophoresis tube constituting the main electrophoresis path (5) has an inner diameter of 200 to 500 μm and a length from the sample injection part (4) to the leading electrode liquid tank (3) of 0.5 m. . Of the two sub-electrophoresis paths (6) and (7),
(6) is 200-500 μm inside diameter, 0.2 m length, (7) is 50 inside diameter
100100 μm and length 0.5 m.
上記リーディング電極液槽（３）及び副リーディング
電極液槽（８）（９）には、同一のリーディング電極液
が充填されている。従って電気泳動前の初期状態では主
電気泳動路（５）のターミナル電極液槽（２）から試料
注入部（４）まではターミナル電極液が充填され、それ
以外の主電気泳動路（５）及び第１・第２の副電気泳動
路（６）（７）にはリーディング電極液が充填されてい
る。ここでターミナル電極液とは易動度の小さいイオン
を含有する電極液であり、リーディング電極液とは易動
度の大きいイオンを含有する電極液である。The leading electrode liquid tank (3) and the sub-leading electrode liquid tanks (8) and (9) are filled with the same leading electrode liquid. Therefore, in the initial state before the electrophoresis, the terminal electrode solution is filled from the terminal electrode solution tank (2) of the main electrophoresis path (5) to the sample injection section (4), and the other main electrophoresis paths (5) and The first and second sub-electrophoresis paths (6) and (7) are filled with a leading electrode solution. Here, the terminal electrode solution is an electrode solution containing ions having low mobility, and the leading electrode solution is an electrode solution containing ions having high mobility.
次に、上記電気泳動装置（１）の作動について説明す
る。Next, the operation of the electrophoresis apparatus (1) will be described.
まず、上記初期状態において、マイクロシリンジで試
料注入部（４）から試料（例えば１〜10μ）をターミ
ナル電極液とリーディング電極液との界面に注入する。First, in the initial state, a sample (for example, 1 to 10 μm) is injected from the sample injection section (4) into the interface between the terminal electrode solution and the leading electrode solution using a microsyringe.
試料注入後、定電流電源と回路切換手段により、電極
（21）（81）間に直流電流（例えば20μＡ程度）を流
す。これにより試料イオン群は電極（31）に向かって主
電気泳動路（５）内を移動すると共に易動度の差に基づ
いて分離される。この試料イオン群の易動度は、ターミ
ナルイオンよりは大きくかつリーディングイオンよりは
小さいので、これらに挟まれて等速電気泳動され、第２
図のごとく各成分ゾーン毎に分離される（該図において
Ｔはターミナルイオン、A,B,Cは試料イオン、Ｌはリー
ディングイオンをそれぞれ示す）。After the sample injection, a DC current (for example, about 20 μA) is passed between the electrodes (21) and (81) by the constant current power supply and the circuit switching means. Thus, the sample ions move toward the electrode (31) in the main electrophoresis path (5) and are separated based on the difference in mobility. Since the mobility of the sample ions is larger than the terminal ions and smaller than the leading ions, the sample ions are subjected to constant-velocity electrophoresis between the ions and the second ions.
As shown in the figure, each component zone is separated (in the figure, T indicates terminal ions, A, B, and C indicate sample ions, and L indicates leading ions).
上記分離成分ゾーン群は、主電気泳動路（５）をさら
に等速電気泳動されて分離がすすめられ、その先端が分
岐接続部イの近傍まで来たとき、検出器（10）により検
出される。このときゾーン泳動に不要な部分（前部）が
第１の副電気泳動路（６）内に電気泳動されて導入され
る（すなわち不要な部分がトラップされる）。The separation component zone group is further subjected to constant-velocity electrophoresis in the main electrophoresis path (5) to be separated, and is detected by the detector (10) when its leading end comes close to the branch connection part a. . At this time, a part (front part) unnecessary for zone migration is electrophoresed into the first sub-electrophoresis path (6) and introduced (that is, an unnecessary part is trapped).
上記のごとく不要部分のトラップ終了後、回路切換手
段の切換え作動により電極（21）（31）間に直流電流
（例えば100μＡ程度）を流す。これにより第１の副電
気泳動路（６）内にトラップされた不要部分はそのまま
該泳動路（６）に残り、一方不要部分が除かれた分離成
分ゾーン群は主電気泳動路（５）をさらに等速電気泳動
されて分離がすすめられ、分岐接続部ロの近傍まで移動
される。この状態でゾーン泳動に必要な成分ゾーンの通
過、つまり成分ゾーンの後部分を検出器（11）により検
出し、そのまま等速電気泳動させて主電気泳動路（５）
内にその成分ゾーンを導入する。As described above, after the trap of the unnecessary portion is completed, a direct current (for example, about 100 μA) flows between the electrodes (21) and (31) by the switching operation of the circuit switching means. As a result, the unnecessary portion trapped in the first sub-electrophoresis path (6) remains in the electrophoresis path (6) as it is, while the separated component zone group from which the unnecessary part has been removed passes through the main electrophoresis path (5). Further, it is subjected to isokinetic electrophoresis to promote separation, and is moved to the vicinity of the branch connection part b. In this state, the detector passes through the component zone necessary for zone migration, that is, the rear part of the component zone is detected by the detector (11), and is electrophoresed at the same speed as it is, and the main electrophoresis path (5)
Introduce the component zone into.
この後、回路切換手段の切換え作動により、電極（3
1）（91）間に直流電流（例えば50μＡ程度）を流す。
これにより、主電気泳動路（５）内のロよりも電極
（３）側にトラップされた目的の成分ゾーンは、第２の
副電気泳動路（７）内で電極（91）に向かってゾーン泳
動されることとなる。ここで分離されたゾーンは検出器
（12）により検出されることとなる。After this, the electrode (3
1) A DC current (for example, about 50 μA) flows between (91).
Thus, the target component zone trapped on the electrode (3) side of the electrode b in the main electrophoresis path (5) moves toward the electrode (91) in the second sub-electrophoresis path (7). Will be electrophoresed. Here, the separated zones are detected by the detector (12).
以上の作動により、まず等速電気泳動で分離された任
意の部分が、さらにゾーン泳動されて検出されることと
なる。By the above operation, first, an arbitrary portion separated by isotachophoresis is further subjected to zone migration and detected.
（ヘ）発明の効果 この発明によれば、希薄試料について一旦等速電気泳
動により任意の試料イオンを濃縮した後、この試料イオ
ンをさらにゾーン泳動することができ、希薄試料を高分
離能のもとに泳動分離できる。従ってこの発明によれ
ば、キャピラリゾーン電気泳動の適用範囲を低濃度試料
まで広げる画期的なの電気泳動装置を提供することがで
きる。(F) Effects of the Invention According to the present invention, once a sample ion is concentrated by isotachophoresis on a dilute sample, the sample ion can be further subjected to zone electrophoresis, and the dilute sample has a high resolution. Can be separated by electrophoresis. Therefore, according to the present invention, it is possible to provide an epoch-making electrophoresis apparatus which extends the applicable range of capillary zone electrophoresis to a low concentration sample.
第１図はこの発明の電気泳動装置の一例の構成説明図、
第２図は第１図の装置の主電気泳動路電気泳動で分離さ
れる試料の分離状態を説明する説明図である。 ２……ターミナル電極液槽、 ３……リーディング電極液槽、 ４……試料注入部、５……主電気泳動路、 6,7……第１・２の副電気泳動路、 8,9……副リーディング電極液槽、 10,11,12……検出器、 21,31,81,91……電極。FIG. 1 is a configuration explanatory view of an example of the electrophoresis apparatus of the present invention,
FIG. 2 is an explanatory diagram for explaining a separation state of a sample separated by main electrophoresis path electrophoresis of the apparatus of FIG. 2 ... Terminal electrode liquid tank, 3 ... Reading electrode liquid tank, 4 ... Sample injection part, 5 ... Main electrophoresis path, 6,7 ... First and second sub-electrophoresis paths, 8,9 ... … Sub reading electrode liquid tank, 10,11,12 …… Detector, 21,31,81,91 …… Electrode.
Claims (1)
電極液槽との間を、試料注入部を介して接続されるキャ
ピラリ電気泳動管で構成される主電気泳動路と、 （２）上記キャピラリ電気泳動管の試料注入部の後段に
別々に分岐接続され、かつ各々副リーディング電極液槽
に接続されたキャピラリ電気泳動管からなる、先に分岐
された第１の副電気泳動路及び後で分岐された第２の副
電気泳動路と、 （３）上記主電気泳動路の各分岐接続部の前段及び上記
第２の副電気泳動路にそれぞれ設けられる検出手段と、 （４）上記第１の副電気泳動路の分岐接続部の前段に設
けられた検出手段により分析対象の成分ゾーンを検出す
るまでターミナル電極液槽と第１の副リーディング電極
液槽との間に、次いで第２の副電気泳動路の前段に設け
られた検出手段により分析対象の成分ゾーンの通過を検
出するまでターミナル電極液槽とリーディング電極液槽
との間に、その後はリーディング電極液槽と第２の副電
気泳動路との間に、それぞれ所定の電圧を印加する電圧
印加手段 とを備えてなる電気泳動装置。(1) a main electrophoresis path composed of a capillary electrophoresis tube connected between a terminal electrode liquid tank and a leading electrode liquid tank via a sample injection section; and (2) the capillary. A first branched first sub-electrophoresis path, which is separately branched after the sample injection part of the electrophoresis tube, and is composed of a capillary electrophoresis tube connected to the sub-leading electrode liquid tank, respectively, and a later branch. (3) detection means provided in the preceding stage of each branch connection part of the main electrophoresis path and in the second sub-electrophoresis path, respectively; and (4) the first sub-electrophoresis path. Until the detection of the component zone to be analyzed is performed by the detection means provided at a stage preceding the branch connection portion of the sub-electrophoresis path, between the terminal electrode liquid tank and the first sub-leading electrode liquid tank, and then the second sub-electrode liquid tank. Provided before the migration path Until the passage of the component zone to be analyzed is detected by the outlet means, between the terminal electrode liquid tank and the leading electrode liquid tank, and thereafter, between the leading electrode liquid tank and the second sub-electrophoresis path, a predetermined amount is set. An electrophoresis apparatus comprising: voltage application means for applying a voltage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1256460A JP2841556B2 (en) | 1989-09-29 | 1989-09-29 | Electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1256460A JP2841556B2 (en) | 1989-09-29 | 1989-09-29 | Electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03118463A JPH03118463A (en) | 1991-05-21 |
| JP2841556B2 true JP2841556B2 (en) | 1998-12-24 |
Family
ID=17292947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1256460A Expired - Fee Related JP2841556B2 (en) | 1989-09-29 | 1989-09-29 | Electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2841556B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050121324A1 (en) * | 2003-09-05 | 2005-06-09 | Caliper Life Sciences, Inc. | Analyte injection system |
| JP2006317357A (en) * | 2005-05-13 | 2006-11-24 | Shimadzu Corp | Microchip electrophoretic method and microchip electrophoretic apparatus |
| EP3300961A1 (en) | 2008-09-30 | 2018-04-04 | TRW Airbag Systems GmbH | Gas generator, method for the production thereof and module having a gas generator |
| KR102198936B1 (en) * | 2019-08-06 | 2021-01-05 | 경희대학교 산학협력단 | Micro-capillary electrophoresis system using step-up converter device |
-
1989
- 1989-09-29 JP JP1256460A patent/JP2841556B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03118463A (en) | 1991-05-21 |
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