JP2938936B2 - Method for measuring antigen or antibody concentration - Google Patents
Method for measuring antigen or antibody concentrationInfo
- Publication number
- JP2938936B2 JP2938936B2 JP15707690A JP15707690A JP2938936B2 JP 2938936 B2 JP2938936 B2 JP 2938936B2 JP 15707690 A JP15707690 A JP 15707690A JP 15707690 A JP15707690 A JP 15707690A JP 2938936 B2 JP2938936 B2 JP 2938936B2
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- antibody
- concentration
- sample
- fine particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、液体試料中の抗原又は抗体の濃度測定方法
に関する。Description: TECHNICAL FIELD The present invention relates to a method for measuring the concentration of an antigen or an antibody in a liquid sample.
〔従来の技術〕 従来、液体試料中の抗原又は抗体の濃度を測定する代
表的な方法としては、レーザーネフェロメトリー、ラテ
ックス比濁法、エンザイムイムノアッセイ、ラジオイム
ノアッセイ等が知られている。[Related Art] Conventionally, as typical methods for measuring the concentration of an antigen or an antibody in a liquid sample, laser nephelometry, latex turbidimetry, enzyme immunoassay, radioimmunoassay, and the like are known.
しかし、前記の抗原又は抗体の濃度測定方法のうち、
レーザーネフェロメトリー及びラテックス比濁法は、試
料液中に血球等の他の共存物質が存在して光の吸収、散
乱が起こる場合には正確な測定を行うことができず、測
定の感度、精度とも大幅に低下するという問題がある。
そのため、測定用の試料の調製には、そのような共存物
質を予め除去する必要があった。また、微粒子表面の凹
凸によって光散乱な状態が変わるため測定結果が影響を
受けるという欠点もあった。However, among the above antigen or antibody concentration measurement methods,
Laser nephelometry and latex turbidimetry cannot perform accurate measurements when light is absorbed or scattered due to the presence of other coexisting substances such as blood cells in the sample solution. There is a problem that the accuracy is greatly reduced.
Therefore, it was necessary to remove such a coexisting substance in advance in preparing a sample for measurement. In addition, there is also a disadvantage that the light scattering state changes due to the unevenness of the fine particle surface, so that the measurement result is affected.
また、エンザイムイムノアッセイでは酵素活性の安定
性という困難な要求があること、そしてラジオイムノア
ッセイでは放射性物質を用いる危険性が伴うという問題
があった。In addition, the enzyme immunoassay has a difficult requirement for stability of enzyme activity, and the radioimmunoassay has the problem of using a radioactive substance.
そこで、本発明の課題は、共存物質が試料液中に共存
していても何らそれに影響されず、したがって、従来の
免疫測定においては分離する必要があった血球等の共存
物質を分離せずに、その存在のままで抗原又は抗体の濃
度を測定することができる方法を提供することにある。Therefore, an object of the present invention is not affected by coexisting substances even if coexisting substances are present in a sample solution, and therefore, without separating coexisting substances such as blood cells that had to be separated in a conventional immunoassay. It is an object of the present invention to provide a method capable of measuring the concentration of an antigen or an antibody in the presence thereof.
本発明者は、かかる課題を解決するものとして、抗原
又は抗体と、抗体又は抗原を固定化した磁気微粒子とを
抗原−抗体反応させて得られる凝集塊の初期磁化特性
が、試料液中の抗体又は抗原の濃度と相関関係があるこ
とを見出した。The present inventor has solved the above-mentioned problem by providing an antigen or antibody, and an antibody or antigen-immobilized magnetic microparticles in an antigen-antibody reaction. Or, it was found that there is a correlation with the concentration of the antigen.
本発明は、かかる発見に基づいて、抗原又は抗体を含
有する液体試料に、該抗原又は抗体と特異的に結合する
抗体又は抗原を固定化した磁気微粒子を懸濁して抗原抗
体反応を起こさせて磁気微粒子を凝集させた後、得られ
た凝集塊に一定方向の磁場を印加しながら該凝集塊の初
期磁化特性を測定する工程を有する、液体試料中の抗原
又は抗体の濃度測定方法を提供するものである。The present invention, based on such a finding, suspends magnetic particles immobilized with an antibody or antigen specifically binding to the antigen or antibody in a liquid sample containing the antigen or antibody to cause an antigen-antibody reaction. Provided is a method for measuring the concentration of an antigen or an antibody in a liquid sample, comprising a step of measuring an initial magnetization characteristic of the aggregate while applying a magnetic field in a certain direction to the obtained aggregate after aggregating the magnetic fine particles. Things.
抗原・抗体固定化磁気微粒子微粒子 この方法において、抗原又は抗体の固定化に用いる磁
気微粒子の材料は、特に限定されず、例えば、Fe3O4,γ
−Fe2O3,Co−γ−Fe2O3,（NiCuZn）Ｏ・Fe2O3,（CuZn）
Ｏ・Fe2O3,（Mn・Zn）Ｏ・Fe2O3,（NiZn）Ｏ・Fe2O3,Sr
O・6Fe2O3,BaO・6Fe2O3,SiO2で被覆したFe2O3（粒径約2
00Å）［Enzyme Microb.Technol.,vol.2,p.2〜10（198
0）参照］、各種の高分子材料（ナイロン、ポリアクリ
ルアミド、タンパク質等）とフェライトとの複合微粒
子、磁性金属微粒子等を挙げることができる。In this method, the material of the magnetic fine particles used for immobilizing the antigen or the antibody is not particularly limited, for example, Fe 3 O 4 , γ
-Fe 2 O 3 , Co-γ-Fe 2 O 3 , (NiCuZn) O ・ Fe 2 O 3 , (CuZn)
O ・ Fe 2 O 3 , (Mn ・ Zn) O ・ Fe 2 O 3 , (NiZn) O ・ Fe 2 O 3 , Sr
O.6Fe 2 O 3 , BaO.6Fe 2 O 3 , Fe 2 O 3 coated with SiO 2 (particle size about 2
00Å) [Enzyme Microb.Technol., Vol. 2, p. 2-10 (198
0)], composite fine particles of various polymer materials (nylon, polyacrylamide, protein, etc.) and ferrite, magnetic metal fine particles, and the like.
磁気微粒子の粒径は、一般に、50〜500Åの範囲でよ
い。The particle size of the magnetic fine particles may generally be in the range of 50 to 500 °.
上記の材料及び粒径を有するものの中でも特に好まし
いものとして、SiO2で被覆した粒径約200ÅのFe3O4粒
子、及び粒径200〜300Åのγ−Fe2O3粒子を挙げること
ができる。さらに、このような好ましい磁気微非粒子と
して、走磁性細菌から得られる磁鉄鉱（Fe3O4）からな
る微粒子（粒径約500Å）が挙げられる。前記走磁性細
菌は、例えば特開昭62−61599号に開示された方法およ
び採取器により淡水又は海水から容易に採取することが
できる。As particularly preferable among those having the above materials and particle size, may be mentioned Fe 3 O 4 particles having a particle size of about 200Å coated with SiO 2, and γ-Fe 2 O 3 particles having a particle diameter 200~300Å . Further, as such preferable magnetic fine non-particles, fine particles (particle diameter: about 500 °) made of magnetite (Fe 3 O 4 ) obtained from magnetotactic bacteria can be mentioned. The magnetotactic bacterium can be easily collected from freshwater or seawater by a method and a collector disclosed in, for example, JP-A-62-61599.
この濃度測定方法に用いられる抗原又は抗体を固定化
した磁気微粒子は、上記の磁気微粒子に所要の抗原又は
抗体を固定化することにより製造することができる。抗
原又は抗体の磁気微粒子への固定化は、抗原又は抗体の
固定化技術として公知の方法により行うことができ、例
えば、シランカップリング剤、ブドウ状球菌より得られ
るプロテインＡを磁気微粒子に被膜させ、そして抗体を
結合させる方法等を用いて行う。The magnetic fine particles having the antigen or antibody immobilized thereon used in this concentration measuring method can be produced by immobilizing the required antigen or antibody on the above magnetic fine particles. The immobilization of the antigen or the antibody on the magnetic microparticles can be performed by a method known as an antigen or antibody immobilization technique.For example, a silane coupling agent, protein A obtained from Staphylococcus is coated on the magnetic microparticles. And a method of binding an antibody.
磁気微粒子に固定される抗体又は抗原の種類は、被測
定対象である特定の抗原又は抗体に対して抗体又は抗原
の関係にあるものであり、試料液中の抗原又は抗体に応
じて選択される。かかる抗原又は抗体の例としては次の
ものを挙げることができる。The type of the antibody or antigen immobilized on the magnetic microparticles is related to the specific antigen or antibody to be measured, and is selected according to the antigen or antibody in the sample solution. . Examples of such antigens or antibodies include the following.
抗原類:IgG、IgA、IgM、IgE、アルブミン、HCG、AF
P、カルジオライピン抗原、血液型物質、コンカナバリ
ンＡ、DNT、プロスタグランジン、CRP、HBs、ヒト成長
ホルモン、ステロイドホルモン、CEA、IgD等。Antigens: IgG, IgA, IgM, IgE, albumin, HCG, AF
P, cardiolipin antigen, blood group substance, concanavalin A, DNT, prostaglandin, CRP, HBs, human growth hormone, steroid hormone, CEA, IgD and the like.
抗体類：抗アルブミン抗体、抗HCG抗体、抗IgG抗体、
抗IgA抗体、抗IgM抗体、抗IgE抗体、抗IgD抗体、抗AFP
抗体、抗DNT抗体、抗プロスタグランジン抗体、抗ヒト
凝固ファクター抗体、抗CRP抗体、抗HBs抗体、抗ヒト成
長ホルモン抗体、抗ステロイドホルモン抗体、およびこ
れらを含む血清、並びにモノクローナル抗体。Antibodies: anti-albumin antibody, anti-HCG antibody, anti-IgG antibody,
Anti-IgA antibody, anti-IgM antibody, anti-IgE antibody, anti-IgD antibody, anti-AFP
Antibodies, anti-DNT antibodies, anti-prostaglandin antibodies, anti-human coagulation factor antibodies, anti-CRP antibodies, anti-HBs antibodies, anti-human growth hormone antibodies, anti-steroid hormone antibodies, sera containing these, and monoclonal antibodies.
抗原抗体反応 上記の抗原又は抗体を固定化した磁気微粒子を試料液
に懸濁する方法は、特に限定されない。一定量の試料液
に、一定濃度で磁気微粒子を含む懸濁液を添加してもよ
いし、あるいはその逆でもよい。磁気微粒子の懸濁液に
用いられる分散媒は、通常、水性媒体が一般的である。Antigen-Antibody Reaction The method for suspending the magnetic fine particles on which the antigen or antibody is immobilized in a sample solution is not particularly limited. A suspension containing magnetic fine particles at a certain concentration may be added to a certain amount of the sample solution, or vice versa. The dispersion medium used for the suspension of the magnetic fine particles is generally an aqueous medium.
抗原又は抗体を固定化した磁気微粒子を、例えば、特
開昭63−90766号公報に開示の懸濁液、即ち、抗原又は
抗体が固定化されている粒径50〜500Åの磁気微粒子
が、該磁気微粒子1mg当り5ml以上の、界面活性剤濃度0.
1重量％以上の等張塩水溶液中に分散してなる懸濁液と
して予め調製しておき、試料液に添加することもでき
る。かかる懸濁液は、安定性が高く保存性が良好である
ので便利である。等張塩水溶液としては、例えば、0.9
％6NaCl溶液、0.025Mしょ糖水溶液を使用することがで
き、また、これに添加する界面活性剤としては、Tween
80、−COOH、COO-などの基を有する界面活性剤等が挙げ
られる。等張塩水溶液中の界面活性剤濃度は、0.1重量
％以上であることが必要で、好ましくは、0.1〜1.0重量
％である。The magnetic fine particles having the antigen or antibody immobilized thereon, for example, the suspension disclosed in Japanese Patent Application Laid-Open No. 63-90766, i.e., the magnetic fine particles having a particle diameter of 50 to 500 mm on which the antigen or antibody is immobilized are used. Surfactant concentration of 5 ml or more per 1 mg of magnetic fine particles.
It may be prepared in advance as a suspension dispersed in an aqueous solution of 1% by weight or more of isotonic salt and added to the sample solution. Such a suspension is convenient because it has high stability and good storage stability. As an isotonic salt aqueous solution, for example, 0.9
% 6NaCl solution and 0.025M sucrose aqueous solution, and as a surfactant to be added thereto, Tween
Surfactants having groups such as 80, -COOH, COO - and the like. The concentration of the surfactant in the aqueous isotonic salt solution needs to be 0.1% by weight or more, and preferably 0.1 to 1.0% by weight.
この方法において、試料液に磁気微粒子を分散させる
には、例えば超音波を利用することができる。試料の懸
濁液中への分散処理により試料液中に存在した抗体又は
抗原は磁気微粒子上に固定化されている抗原又は抗体に
結合し、抗原−抗体−磁気微粒子からなる三元結合体を
生成する。これらの結合体は、抗原−抗体反応の進行に
より隣接する結合体同士で凝集し、凝集塊を生成する。In this method, for example, ultrasonic waves can be used to disperse the magnetic fine particles in the sample liquid. The antibody or antigen present in the sample solution by the dispersion treatment of the sample in the suspension binds to the antigen or antibody immobilized on the magnetic fine particles to form a ternary conjugate consisting of an antigen-antibody-magnetic fine particles. Generate. These conjugates agglutinate between adjacent conjugates due to the progress of the antigen-antibody reaction to form aggregates.
このとき、特開昭63−90766号公報に記載のように試
料液に磁界を適用してもよく、これにより凝集塊の生成
を促進することができる。At this time, a magnetic field may be applied to the sample liquid as described in JP-A-63-90766, whereby the formation of aggregates can be promoted.
初期磁化特性の測定 本発明の方法において、凝集塊の初期磁化特性の測定
は地磁気の影響を極力避けうる装置によって行うことが
求められ、好ましい磁力計としては、例えば、振動試料
型磁力計（VSM）を使用することができる。Measurement of Initial Magnetization Characteristics In the method of the present invention, the measurement of the initial magnetization characteristics of the agglomerates is required to be performed by an apparatus that can minimize the influence of geomagnetism. As a preferable magnetometer, for example, a vibrating sample magnetometer (VSM ) Can be used.
前記の抗原−抗体反応により得られる凝集塊の粒度
は、試料液中の抗原又は抗体の濃度と相関関係がある。
即ち、試料中の抗原又は抗体の濃度が高いほど生成する
凝集塊の粒度が大きくなる。こうして得られた凝集塊の
分散液に一定方向の磁場を印加すると各凝集塊は磁場の
方向に配向させられるが、凝集塊の粒度が小さいほど配
向が速いため磁化曲線の上昇する傾きが鋭くなる。一
方、凝集塊の粒度が大きいほど磁化曲線の傾きは小さく
なる。したがって、既知の抗原又は抗体の濃度に対する
初期磁化特性の変化を、例えば検量線として求め、これ
に基づいて未知の抗原又は抗体の濃度を測定することが
できる。The particle size of the aggregate obtained by the antigen-antibody reaction has a correlation with the antigen or antibody concentration in the sample solution.
That is, the higher the concentration of the antigen or antibody in the sample, the larger the particle size of the formed aggregate. When a magnetic field in a certain direction is applied to the thus-obtained dispersion of aggregates, each aggregate is oriented in the direction of the magnetic field. . On the other hand, the larger the particle size of the aggregate, the smaller the slope of the magnetization curve. Therefore, a change in the initial magnetization characteristics with respect to the concentration of the known antigen or antibody can be determined, for example, as a calibration curve, and the concentration of the unknown antigen or antibody can be measured based on the curve.
実施例１ （１）粒径150Åの酸化鉄からなる磁気微粒子に抗ヒトI
gGを固定化したものを、Tween80を0.6％含む生理食塩水
に濃度1mg/mlで分散させ、磁気微粒子懸濁液を調製し
た。Example 1 (1) Anti-human I was applied to magnetic fine particles of iron oxide having a particle size of 150 °.
The immobilized gG was dispersed at a concentration of 1 mg / ml in physiological saline containing 0.6% Tween80 to prepare a suspension of magnetic fine particles.
（２）次に、抗原であるヒトIgGを、それぞれ、０、
１、10、100又は1000μg/ml含む５種の試料水溶液各0.1
mlに、前記の磁気微粒子分散液0.1mlを添加した。これ
らの懸濁液に電磁石を用いて周波数2Hzの交番磁場200G
程度を５分間印加し、抗原−抗体反応を完全に行わせ、
凝集塊を生成させた。(2) Next, human IgG as an antigen was
Five kinds of sample aqueous solutions containing 1, 10, 100 or 1000 μg / ml each 0.1
To 0.1 ml, 0.1 ml of the above-mentioned magnetic fine particle dispersion was added. Using an electromagnet on these suspensions, an alternating magnetic field of 200 Hz with a frequency of 2 Hz
5 minutes applied to complete the antigen-antibody reaction,
Agglomerates formed.
次に、試料液をアクリル樹脂製の直径7mm、厚さ5mm、
内容量40μの円柱状容器、サンプルホルダーに移し、
振動試料型磁力計（理研電子（株）製,BHV−３）で、初
期磁化曲線を求めたところ図１に示す結果が得られた。Next, the sample solution was made of acrylic resin with a diameter of 7 mm, a thickness of 5 mm,
Transfer to a cylindrical container with a content of 40μ, sample holder,
When the initial magnetization curve was determined using a vibrating sample magnetometer (manufactured by Riken Denshi Co., Ltd., BHV-3), the results shown in FIG. 1 were obtained.
実施例２ 実施例１の（１）で示した方法によって濃度10mg/ml
の磁気微粒子懸濁液を調製した。次に、実施例１の
（２）と同様にして抗原−抗体反応を完全に行わせて凝
集塊を生成させ、測定を行った。Example 2 The concentration was 10 mg / ml according to the method shown in (1) of Example 1.
Was prepared. Next, the antigen-antibody reaction was completely performed in the same manner as in Example 1 (2) to form an aggregate and measurement was performed.
そして、磁場200 Oeにおける磁化を各抗原濃度ごとに
求めたところ、図２に示す結果が得られた。When the magnetization at a magnetic field of 200 Oe was determined for each antigen concentration, the results shown in FIG. 2 were obtained.
実施例３ ヒトIgGを種々の既知濃度で含む５種の試料水溶液各
0.1mlに、前記の実施例１の（１）で調製した磁気微粒
子分散液0.1mlを添加した。実施例１と同様にして抗原
−抗体反応を完全に行わせ、凝集塊を生成させた。Example 3 Five types of aqueous sample solutions containing human IgG at various known concentrations
To 0.1 ml, 0.1 ml of the magnetic fine particle dispersion prepared in (1) of Example 1 was added. The antigen-antibody reaction was completely performed in the same manner as in Example 1 to generate an aggregate.
次に、試料液を前記サンプルホルダーに移し、振動試
料型磁力計（理研電子（株）製,BHV−３）で、磁場200
Oeにおける磁化を各抗原濃度ごとに求めたところ、図３
に示す検量線が得られた。Next, the sample liquid was transferred to the sample holder, and a magnetic field of 200 μm was measured using a vibrating sample magnetometer (BHV-3, manufactured by Riken Denshi Co., Ltd.).
Figure 3 shows the magnetization in Oe for each antigen concentration.
Was obtained.
本発明の抗原又は抗体の濃度測定方法は、血球等の共
存物質の存在下においても何ら影響されずに抗原抗体反
応による磁気微粒子の粒度を短時間で測定できる、酵素
を使用しないためその安定性が問題とならず、また放射
性物質を使用しないので安全性が高い等の点で有利であ
る。The antigen or antibody concentration measuring method of the present invention can measure the particle size of magnetic fine particles by an antigen-antibody reaction in a short time without any influence even in the presence of coexisting substances such as blood cells. Is not a problem, and the use of radioactive materials is advantageous in that safety is high.
図１は、実施例１で測定された各抗原濃度の試料につい
ての初期磁化曲線を示し、図２は、同じく200 Oeにおけ
る抗原濃度と磁化変化率の関係を示す。 図３は、実施例２で得られた磁場200 Oeにおける抗原濃
度と磁化変化率の関係を示す。FIG. 1 shows an initial magnetization curve for each sample of each antigen concentration measured in Example 1, and FIG. 2 shows a relationship between the antigen concentration and the magnetization change rate at 200 Oe. FIG. 3 shows the relationship between the antigen concentration and the rate of change in magnetization in a magnetic field of 200 Oe obtained in Example 2.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭63−90765（ＪＰ，Ａ) 特開 昭63−108264（ＪＰ，Ａ) 特開 昭63−302367（ＪＰ，Ａ) 特開 昭60−55265（ＪＰ，Ａ) 特開 平１−112161（ＪＰ，Ａ) 特開 昭63−90766（ＪＰ，Ａ) 特開 昭62−287159（ＪＰ，Ａ) (58)調査した分野(Int.Cl.6，ＤＢ名) G01N 33/553,33/543 ──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-63-90765 (JP, A) JP-A-63-108264 (JP, A) JP-A-63-302367 (JP, A) JP-A-60-1985 55265 (JP, A) JP-A-1-112161 (JP, A) JP-A-63-90766 (JP, A) JP-A-62-287159 (JP, A) (58) Fields investigated (Int. Cl. 6 , DB name) G01N 33/553, 33/543
Claims (2)
抗原又は抗体と特異的に結合する抗体又は抗原を固定化
した磁気微粒子を懸濁して抗原抗体反応を起こさせて磁
気微粒子を凝集させた後、得られた凝集塊を含む系に一
定方向の磁場を印加しながら該凝集塊の初期磁化特性を
測定する工程を有する、液体試料中の抗原又は抗体の濃
度測定方法。An antigen or antibody which specifically binds to the antigen or antibody is suspended in a liquid sample containing the antigen or antibody to cause an antigen-antibody reaction to aggregate the magnetic particles. And thereafter measuring the initial magnetization characteristics of the aggregate while applying a magnetic field in a certain direction to the obtained system containing the aggregates.
る請求項（１）の方法。2. The method according to claim 1, wherein a magnetic field is applied to the reaction system during the antigen-antibody reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15707690A JP2938936B2 (en) | 1990-06-15 | 1990-06-15 | Method for measuring antigen or antibody concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15707690A JP2938936B2 (en) | 1990-06-15 | 1990-06-15 | Method for measuring antigen or antibody concentration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0448266A JPH0448266A (en) | 1992-02-18 |
| JP2938936B2 true JP2938936B2 (en) | 1999-08-25 |
Family
ID=15641720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15707690A Expired - Fee Related JP2938936B2 (en) | 1990-06-15 | 1990-06-15 | Method for measuring antigen or antibody concentration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2938936B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6468809B1 (en) * | 2000-02-04 | 2002-10-22 | The United States Of America As Represented By The Secretary Of The Navy | High efficiency magnetic sensor for magnetic particles |
| JP6926907B2 (en) | 2017-09-29 | 2021-08-25 | コニカミノルタ株式会社 | Antibody dispersion for measurement, its production method, antibody dispersion preparation kit for measurement, and method for measuring biological substances |
| JP7171702B2 (en) * | 2018-03-23 | 2022-11-15 | 大塚製薬株式会社 | Labeled antibody dispersion, SPFS kit |
| CN110780079A (en) * | 2019-11-28 | 2020-02-11 | 南京迪安医学检验所有限公司 | Squamous cell carcinoma antigen detection reagent |
-
1990
- 1990-06-15 JP JP15707690A patent/JP2938936B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0448266A (en) | 1992-02-18 |
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