JP2847529B2 - Method for improving antibacterial property of silver-containing compound - Google Patents
Method for improving antibacterial property of silver-containing compoundInfo
- Publication number
- JP2847529B2 JP2847529B2 JP17139189A JP17139189A JP2847529B2 JP 2847529 B2 JP2847529 B2 JP 2847529B2 JP 17139189 A JP17139189 A JP 17139189A JP 17139189 A JP17139189 A JP 17139189A JP 2847529 B2 JP2847529 B2 JP 2847529B2
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- Prior art keywords
- silver
- phosphate
- containing compound
- antibacterial
- sodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Cosmetics (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は銀含有化合物の抗菌性向上方法及びこの方法
を利用した抗菌剤に関する。本発明の方法及び抗菌剤を
用いて優れた抗菌性を有する医薬品及び抗菌性高分子体
等を提供することができる。The present invention relates to a method for improving the antibacterial property of a silver-containing compound and an antibacterial agent utilizing the method. By using the method and the antibacterial agent of the present invention, it is possible to provide a drug and an antibacterial polymer having excellent antibacterial properties.
銀が抗菌性を有する金属であることは古くから知られ
ている。そこでこの効果をより有効に利用するために、
アルミノケイ酸塩やイオン交換樹脂等に銀イオンの形で
保持させて抗菌性を長期間持続させることも知られてい
る（特開昭60−181002号、特開昭58−156074号）。さら
にこれら銀イオンを保持したアルミノケイ酸塩を高分子
体に練り込み抗菌性高分子体を調製して種々の抗菌性の
成形品を提供できる（特開昭61−138658号）。It has long been known that silver is a metal having antibacterial properties. So, in order to use this effect more effectively,
It is also known that the antibacterial property is maintained for a long period of time by being retained in the form of silver ions in an aluminosilicate or an ion exchange resin (JP-A-60-181002, JP-A-58-156074). Furthermore, various antibacterial molded articles can be provided by kneading the aluminosilicate holding silver ions into a polymer to prepare an antibacterial polymer (JP-A-61-138658).
しかし銀の抗菌効果は、含有される銀イオンの量によ
り変動するが、大腸菌の最小発育阻止濃度（MIC）で100
〜500ppm程度である。このMIC値は、従来より知られて
いる抗生物質のMIC値１−10ppmに比べてかなり大きいも
のである。そのため銀あるいは銀の化合物を高分子体に
練り込み抗菌性高分子体を得る場合も0.4〜５重量％程
度添加する必要があり、効率的とは言えなかった。However, the antibacterial effect of silver varies depending on the amount of silver ions contained.
About 500 ppm. This MIC value is considerably larger than the MIC value of a conventionally known antibiotic of 1 to 10 ppm. Therefore, when kneading silver or a silver compound into a polymer to obtain an antibacterial polymer, it is necessary to add about 0.4 to 5% by weight, which is not efficient.
そこで、本発明の目的は、銀あるいは銀の化合物の抗
菌性を抗生物質とほぼ同程度の抗菌効果に向上させる方
法及びこの方法を用いた抗菌剤を提供することにある。Accordingly, an object of the present invention is to provide a method for improving the antibacterial activity of silver or a silver compound to an antibacterial effect substantially equivalent to that of an antibiotic, and an antibacterial agent using this method.
本発明者等は、微生物のエネルギー代謝や細胞レベル
の銀イオン挙動などを鋭意研究した。その結果、銀含有
化合物にリン酸含有化合物を共存させることにより銀含
有化合物が本来有する抗菌性より高い抗菌性が得られる
ことを見い出し本発明を完成するに至った。The present inventors have intensively studied the energy metabolism of microorganisms and the behavior of silver ions at the cell level. As a result, they have found that the coexistence of the phosphoric acid-containing compound with the silver-containing compound can provide a higher antibacterial property than the silver-containing compound originally has, and completed the present invention.
そこで本発明は、銀含有化合物の抗菌性を向上させる
方法であって、銀含有化合物にリン酸含有化合物を共存
させることを特徴とする上記方法に関する。Therefore, the present invention relates to a method for improving the antibacterial property of a silver-containing compound, wherein the phosphoric acid-containing compound is allowed to coexist with the silver-containing compound.
さらに本発明は、銀含有化合物及びリン酸含有化合物
を含む抗菌剤並びに銀及びリン酸を含有する化合物を含
む抗菌剤を提供するものである。Further, the present invention provides an antibacterial agent containing a silver-containing compound and a phosphoric acid-containing compound and an antibacterial agent containing a compound containing silver and phosphoric acid.
以下本発明について詳細に説明する。 Hereinafter, the present invention will be described in detail.
本発明において銀含有化合物は、硝酸銀、硫酸銀、過
塩素酸銀、酢酸銀、塩化銀やジアミン銀硝酸塩、ジアン
ミン銀硫酸塩等の銀を含む錯塩、銀担持活性炭、銀担持
ゼオライト、銀担持無定形アルミノケイ酸塩等の銀イオ
ンを保持した担体、銀含有ガラス、銀含有固溶体、銀ク
ラスター、銀合金、金属銀、銀含有鉱物等を挙げること
ができる。In the present invention, the silver-containing compound may be silver nitrate, silver sulfate, silver perchlorate, silver acetate, a complex salt containing silver such as silver chloride, diamine silver nitrate, or diammine silver sulfate; silver-supported activated carbon; silver-supported zeolite; Examples of the carrier include silver ion-supported carriers such as aluminosilicates, silver-containing glasses, silver-containing solid solutions, silver clusters, silver alloys, metallic silver, and silver-containing minerals.
一方、リン酸含有化合物は、オルトリン酸、メタリン
酸、ピロリン酸、無水リン酸、リン酸水素ナトリウム、
リン酸カリウム、リン酸カルシウム、リン酸アンモニウ
ム、リン酸マグネシウムアンモニウム、トリポリリン酸
ナトリウム、ヘキサメタリン酸ナトリウム、リンタング
ステン酸ナトリウム、リンモリブデン酸アンモニウム等
の無機リン酸化合物、リン酸−ｎ−トリブチル、リン酸
トリクレシル、リン酸トリフェニル、グルコース−１−
リン酸、トリエチルホスフェート、トリメチルホスフェ
ート、トリブチルホスフェート、エチルアシッドホスフ
ェート、ジアルキルジチオリン酸、トリメチルホスファ
イト、チオリン酸エステル、グルコース−６−リン酸、
Ｄ−フルクトース−６−リン酸等の有機リン酸化合物、
ホスファチジルコリン（レシチン）、プラスマロゲン、
エーテルリピド、カルジオリピン等のリン脂質を挙げる
ことができる。On the other hand, the phosphoric acid-containing compound is orthophosphoric acid, metaphosphoric acid, pyrophosphoric acid, phosphoric anhydride, sodium hydrogen phosphate,
Inorganic phosphate compounds such as potassium phosphate, calcium phosphate, ammonium phosphate, magnesium ammonium phosphate, sodium tripolyphosphate, sodium hexametaphosphate, sodium phosphotungstate, ammonium phosphomolybdate, -n-tributyl phosphate, tricresyl phosphate, Triphenyl phosphate, glucose-1-
Phosphoric acid, triethyl phosphate, trimethyl phosphate, tributyl phosphate, ethyl acid phosphate, dialkyl dithiophosphoric acid, trimethyl phosphite, thiophosphate, glucose-6-phosphate,
An organic phosphate compound such as D-fructose-6-phosphate,
Phosphatidylcholine (lecithin), plasmalogen,
Examples include phospholipids such as ether lipids and cardiolipin.
本発明の方法及び抗菌剤においては、重量比で、銀含
有化合物を銀として１に対してリンとして１〜50、好ま
しくは１〜10のリン酸含有化合物を共存又は配合するこ
とが優れた抗菌効果を得るという観点から望ましい。In the method and the antibacterial agent of the present invention, an antibacterial agent which is excellent in coexistence or incorporation of 1 to 50, preferably 1 to 10 phosphoric acid-containing compounds as phosphorus with respect to 1 as a silver-containing compound in terms of silver by weight is excellent. It is desirable from the viewpoint of obtaining the effect.
さらに、本発明で用いる銀及びリン酸を含有する化合
物としては、リン酸銀（Ag3PO4）、ピロリン酸銀（Ag4P
2O7）、メタリン酸銀（AgPO3）、リン酸ウラニル銀（Ag
UO2PO4）、リン酸水素銀（Ag2HPO4）、リン酸銀アンモ
ニウム（Ag2(NH4)PO4）、リンタングステン酸銀（Ag
6［(PO4W9O27)2］）、リンモリブデン酸銀（Ag6［（PO4
Mo9O27）］2等を例示でき、内でもリン酸銀、ピロリン
酸銀及びリン酸銀アンモニウムが好ましい。Further, the compounds containing silver and phosphoric acid used in the present invention include silver phosphate (Ag 3 PO 4 ), silver pyrophosphate (Ag 4 P
2 O 7 ), silver metaphosphate (AgPO 3 ), silver uranyl phosphate (Ag
UO 2 PO 4 ), silver hydrogen phosphate (Ag 2 HPO 4 ), ammonium silver phosphate (Ag 2 (NH 4 ) PO 4 ), silver phosphotungstate (Ag
6 [(PO 4 W 9 O 27 ) 2 ]), silver phosphomolybdate (Ag 6 [(PO 4
Mo 9 O 27 )] 2 and the like, among which silver phosphate, silver pyrophosphate and ammonium silver phosphate are preferred.
本発明の方法及び抗菌剤の抗微生物特性は各種環境下
で異なるがMIC値として銀量が約30ppmである。さらに、
どの様な場合でも約100ppmあれば充分な効果が発揮され
る。The antimicrobial properties of the method and the antibacterial agent of the present invention are different in various environments, but the MIC value is about 30 ppm of silver. further,
In any case, about 100 ppm can provide a sufficient effect.
本発明の方法が効果的であるのは、リン酸イオンが抗
菌性を有する銀イオンを効率的に細胞内に取り込むため
と考えられるが、このような理論に拘泥するものではな
い。It is considered that the method of the present invention is effective because phosphate ions efficiently take in silver ions having antibacterial properties into cells, but it is not limited to such a theory.
本発明の抗菌剤の形態は液体、エマルジョン、サスペ
ンション等をはじめ粉体、ゲル体、粒状体、抄紙体、ペ
レット体、シート、フィルム等の成型体、スプレー、多
孔質体、繊維体さらにそれらを不織布、発泡シート、
紙、プラスチック、無機質板などの担持体と組みあわせ
た形態とすることもできる。The form of the antibacterial agent of the present invention includes liquids, emulsions, suspensions and other powders, gels, granules, papers, pellets, sheets, films and other molded products, sprays, porous materials, fibrous materials, and the like. Non-woven fabric, foam sheet,
It can also be in the form of a combination with a support such as paper, plastic, or an inorganic plate.
本発明の方法及び抗菌剤は、医薬品及び抗菌性高分子
体等の種々の分野に利用できる。例えば、医療分野、農
林水産分野、化粧品分野、食品加工分野、繊維衣料分
野、寝装分野、建材分野、船舶分野、電子工業分野、水
処理分野等に使用できる。医療分野には軟膏剤、ゲル
剤、油状懸濁剤、乳剤等の種々の形状の外用消毒薬、カ
テーテル、鉗子、リネン類、測定機器等の人以外の医療
環境消毒薬；農林水産分野は農産物病原菌殺菌剤、畜舎
消毒薬、生けす用漁網防汚剤、化粧品分野は化粧料防腐
剤、毛髪洗浄剤；食品加工分野は食品工場装置、作業
衣、プラスチック、紙等の食品包装材、食品保存料；繊
維衣料分野は衛生加工ソックス、トレーニングウエア
ー、病院衣、手術衣、寝装分野はベットカバー、シー
ツ、布団、毛布、建材分野は壁・床用塗料、接着剤、家
具類内張、シャワーカーテン；船舶分野は船底塗料、水
中構造物用防藻防具塗料；電子工業分野は電子部品包装
材、電子工業用薬品殺菌剤；水処理分野は上下水道殺菌
剤、浄水器、冷風扇、冷却塔、配管、濾過膜、フィルタ
ー、充填物のスライムコントロール剤、水溶性金属加工
油防腐剤等に使用するのが好ましい。The method and antibacterial agent of the present invention can be used in various fields such as pharmaceuticals and antibacterial polymers. For example, it can be used in the fields of medicine, agriculture, forestry and fisheries, cosmetics, food processing, textile and clothing, bedding, construction materials, ships, electronics, water treatment, and the like. In the medical field, external disinfectants in various forms such as ointments, gels, oily suspensions, emulsions, etc., and medical environmental disinfectants for non-humans such as catheters, forceps, linens, measuring instruments, etc .; Disinfectants for pathogens, livestock disinfectants, fishing net antifouling agents for livestock, cosmetics in the cosmetics field, cosmetic preservatives, hair cleaners; food processing in the food processing equipment, working clothes, plastic packaging, food packaging materials such as paper, food preservation Materials: Sanitary processing socks, training garments, hospital garments, surgical gowns in the textile clothing field, bed covers, sheets, futons, blankets in the bedding field, paints for walls and floors, adhesives, furniture linings, showers in the building materials field Curtains: Ships: Ship bottom paints, anti-algae paints for underwater structures; Electronic industry: Electronic component packaging materials, chemical disinfectants for electronic industry; Water treatment: water and sewage disinfectants, water purifiers, cold air fans, cooling towers , Piping, filtration membrane, fill Chromatography, slime control agent of the packing are preferably used for the water-soluble metalworking fluid preservatives and the like.
本発明の方法及び抗菌剤を用いた抗菌性高分子体とす
ることができる高分子体としては例えばポリエチレン、
ポリプロピレン、塩化ビニル樹脂、ABS樹脂、ナイロ
ン、ポリエステル、ポリ塩化ビニリデン、ポリスチレ
ン、ポリアセタール、ポリカーボネート、アクリル樹
脂、ふっ素樹脂、ポリウレタンエラストマー、ポリエス
テルエラストマー、フェノール樹脂、ユリア樹脂、メラ
ミン樹脂、不飽和ポリエステル樹脂、エポキシ樹脂、ウ
レタン樹脂、天然及び合成ゴムなどの熱可塑性又は熱硬
化性樹脂を挙げることができる。抗菌剤の添加量は高分
子体に対して重量パーセントで0.01〜３％、好ましくは
0.05〜0.5％とすることが適当である。Examples of the polymer that can be an antibacterial polymer using the method and the antibacterial agent of the present invention include polyethylene,
Polypropylene, vinyl chloride resin, ABS resin, nylon, polyester, polyvinylidene chloride, polystyrene, polyacetal, polycarbonate, acrylic resin, fluorine resin, polyurethane elastomer, polyester elastomer, phenol resin, urea resin, melamine resin, unsaturated polyester resin, epoxy Thermoplastic or thermosetting resins such as resins, urethane resins, natural and synthetic rubbers can be mentioned. The amount of the antimicrobial agent added is 0.01 to 3% by weight relative to the polymer, preferably
It is appropriate to set it to 0.05 to 0.5%.
又、本発明の方法及び抗菌剤を医薬品として利用する
場合には、主成分である銀含有化合物の公知の使用形態
に合せるのが適当である。例えば経口投与、経皮投与、
経腸投与等が挙げられ、特に外用薬として、水溶液ある
いはアルコール、グリセリンの溶液としたり、軟膏、ク
リーム剤又はハップ剤等とすることができる。When the method and the antibacterial agent of the present invention are used as pharmaceuticals, it is appropriate to match the known use form of the silver-containing compound as a main component. For example, oral administration, transdermal administration,
Enteral administration and the like can be mentioned. In particular, an external preparation can be an aqueous solution or a solution of alcohol or glycerin, an ointment, a cream or a cataplasm.
本発明の方法及び抗菌剤で効果の発揮される微生物は
銀含有化合物が本来抗菌性を発揮するものであり、例え
ば、大腸菌（Escherichia coli）、シュードモナス・ア
エルギノサ（Pseudomonas aeruginosa）、スタフィロコ
ッカス・アウレウス（Staphyloccocus aureus）、スト
レプトコッカス・ファエカリス（Streptococcus faecal
is）、ビブリオ・パラハエムディチカス（Vibrio parah
aemdyticus）、カンディダ・アルビカンサ（Candida al
bicans）、アスペルギラス・フラバス（Aspergillus fl
avas）、ペニシリウム・シトリナム（Penicillium citr
inum）、トリコデルマ・ビリデ（Trichoderma virid
e）、プロピオニ・バクテリウム（Propionibacteriu
m）、クラミディア・トラコマチス（Chlamydia trachom
atis）等があり、細菌、真菌（カビ）、酵母、放線菌、
リケッチア、ウィルス等ほとんどの微生物が含まれる。The microorganisms which are effective by the method and the antibacterial agent of the present invention are those in which a silver-containing compound inherently exerts antibacterial properties. For example, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus (Staphyloccocus aureus), Streptococcus faecals (Streptococcus faecal)
is), Vibrio parahedicus
aemdyticus), Candida albicans (Candida al
bicans), Aspergillus flava (Aspergillus fl)
avas), Penicillium citrnum
inum), Trichoderma virid
e), Propionibacteriu
m), Chlamydia trachom
atis), bacteria, fungi (mold), yeast, actinomycetes,
Most microorganisms such as rickettsia and viruses are included.
本発明の方法及び抗菌剤は、銀含有化合物が本来有す
る抗菌性を向上させるものであり、抗生物質とほぼ同程
度の抗菌効果を提供できる。その結果、本発明の方法及
び抗菌剤は、医薬品及び抗菌性高分子体等に利用するこ
とができる。The method and antibacterial agent of the present invention improve the intrinsic antibacterial property of the silver-containing compound, and can provide almost the same antibacterial effect as an antibiotic. As a result, the method and antibacterial agent of the present invention can be used for pharmaceuticals, antibacterial polymers, and the like.
次に本発明を実施例により説明する。 Next, the present invention will be described with reference to examples.
実施例１（抗菌剤の調製） 銀含有化合物、リン酸含有化合物及び担体、媒体等を
配合し各種抗菌剤を調製した。表１に配合比を示す。表
１中No.1−１〜１−４は各液を混合した。No.1−５〜１
−８は担体と硝酸銀溶液を反応させ、銀を担持させ、乾
燥させたものとリン酸化合物を混合した。No.1−９は１
−８で調製した銀担持ゼオライトをさらに配合した。N
o.1−10は、特公昭46−28099に準拠して作成した銀２％
含有ガラスを、No.1−11は鹿児島県菱刈鉱山産出の銀0.
05％含有の鉱石をそれぞれ混合した。また比較例として
リン酸化合物の含まない例も調製した。Example 1 (Preparation of antibacterial agent) Various antibacterial agents were prepared by mixing a silver-containing compound, a phosphoric acid-containing compound, a carrier, a medium, and the like. Table 1 shows the compounding ratio. No. 1-1 to 1-4 in Table 1 mixed each liquid. No.1-5-1
-8 was obtained by reacting a carrier with a silver nitrate solution, carrying silver, and drying and mixing a phosphoric acid compound. No.1-9 is 1
The silver-supported zeolite prepared in -8 was further blended. N
o.1-10 is silver 2% prepared in accordance with JP-B-46-28099.
The glass containing No. 1-11 is silver 0 from Hishikari mine, Kagoshima Prefecture.
Each ore containing 05% was mixed. As a comparative example, an example containing no phosphoric acid compound was also prepared.
実施例２（抗菌剤含有高分子体の成形） 実施例１で調製した抗菌剤（粉体）を各種高分子体と
配合し、射出成型機にて試作プレート（60×35×2mm）
を作成した。配合比及び成型条件を表２に示す。 Example 2 (Molding of antibacterial agent-containing polymer) The antibacterial agent (powder) prepared in Example 1 was blended with various polymers, and a prototype plate (60 × 35 × 2 mm) was injected with an injection molding machine.
It was created. Table 2 shows the mixing ratio and molding conditions.
実施例３（抗菌剤スプレーの作成） 実験No.1−９で調製したフェニルアルコール懸濁液80
mlを金属スプレー缶にフレオンガスと共に封入し、抗菌
剤スプレーを作成した。また比較例として抗菌剤を全く
含まないスプレーも作成した。 Example 3 (Preparation of antibacterial agent spray) Phenyl alcohol suspension 80 prepared in Experiment No. 1-9
ml was sealed in a metal spray can along with freon gas to make an antimicrobial spray. As a comparative example, a spray containing no antibacterial agent was also prepared.
実施例４（抗菌剤軟膏の作成） 実験No.1−６、１−８で調製した抗菌剤を下記の軟膏
構成物質に加えて外用軟膏を作成した。軟膏は80℃とし
た水に抗菌剤とグリセリンをよく混合した懸濁物を加
え、さらに70℃に加温したシトステアリルアルコール、
白色ワセリン、さらに流動パラフィンを加え作成した。
また比較例として抗菌剤を全く含まない軟膏も作成し
た。Example 4 (Preparation of antimicrobial ointment) The antimicrobial agents prepared in Experiment Nos. 1-6 and 1-8 were added to the following ointment components to prepare external ointments. Ointment was added to 80 ° C water with a well-mixed suspension of antibacterial agent and glycerin, and further heated to 70 ° C for sitosteryl alcohol,
White petrolatum and liquid paraffin were added to make it.
An ointment containing no antibacterial agent was also prepared as a comparative example.
（軟膏配合） 流動パラフィン 10部 シトステアリルアルコール ５部 白色ワセリン 20部 グリセリン 15部 水 50部 抗菌剤 １部 試験例１（MIC測定試験） 実施例１で調製した抗菌剤の抗菌効果についてMICに
て評価した。測定方法は試験菌株としてシュードモナス
・アエルギノサ（Pseudomonas aeruginosa）IID P−
１、アスペルギラス・ニガー（Aspergillus niger）IFO
4407、カンディダ・アルビカンサ（Candida albican
s）IFO 1594の３種類を使用した。滅菌精製水で各抗菌
剤の希釈段階懸濁液を調製し、これを溶解後50〜60℃と
なった感受性測定用培地に、培地の1/9量加えて充分混
合後シャーレに分注、固化させて感受性測定用平板とし
た。感受性測定用培地は、細菌類にはMueller Hinton M
edium培地、真菌・酵母類にはサブロー寒天培地をそれ
ぞれ用いた。接種菌液は106/mlに調整したものを用い
た。接種菌液を感受性測定用平板に白金耳で2cm間隔で
線状に塗抹し、細菌類は37℃、20時間、真菌・酵母類は
25℃、７日間培養した。判定は培養後の菌数の変化を測
定し、初期菌数より102以上低下した状態の濃度をMICと
した。結果を表３に示す。比較例１〜３及び抗生物質
（ストレプトマイシン、三共製；比較例４）も同様に試
験した。(Ointment formulation) Liquid paraffin 10 parts Sitosteryl alcohol 5 parts White petrolatum 20 parts Glycerin 15 parts Water 50 parts Antibacterial agent 1 part Test example 1 (MIC measurement test) Antimicrobial effect of the antimicrobial agent prepared in Example 1 was measured by MIC evaluated. The assay was performed using Pseudomonas aeruginosa IID P-
1. Aspergillus niger IFO
4407, Candida albican
s) Three types of IFO 1594 were used. Prepare a dilution step suspension of each antibacterial agent with sterile purified water, dissolve this in a culture medium for sensitivity measurement at 50-60 ° C, add 1/9 of the medium, mix well, and dispense into petri dishes, It was solidified to obtain a plate for sensitivity measurement. The sensitivity measurement medium is Mueller Hinton M for bacteria.
An edium medium and a Sabouraud agar medium were used for fungi and yeasts, respectively. The inoculum was adjusted to 10 6 / ml. The inoculum is smeared linearly at intervals of 2 cm with a platinum loop on a plate for sensitivity measurement, bacteria are 37 ° C, 20 hours, fungi and yeast are
The cells were cultured at 25 ° C for 7 days. Judgment was made by measuring the change in the number of bacteria after culturing, and the concentration in a state where the number was reduced by 10 2 or more from the initial number of bacteria was defined as MIC. Table 3 shows the results. Comparative Examples 1 to 3 and an antibiotic (Streptomycin, manufactured by Sankyo; Comparative Example 4) were similarly tested.
試験例２（高分子成形品の抗菌性試験） 実施例２で形成した高分子体を50×50mmに切断し、そ
れぞれについて大腸菌（Escherichia coli）菌数（105
個/ml）及びシュードモナス・アエルギノサ（Pseudomon
as aeruginosa）菌液（105個/ml）をおのおの15ml降り
掛け、37℃で18時間培養した。菌液を生理食塩水にて洗
い流し、この液について存在する大腸菌数及び緑膿菌数
を測定した。結果を表２に示す。一般に18時間後の菌数
が加えた初期菌数により２桁以上減少した場合に抗菌性
を有するといえる。 Test Example 2 (Antibacterial test of polymer molded article) The polymer formed in Example 2 was cut into 50 x 50 mm, and the Escherichia coli count (10 5
/ Ml) and Pseudomonas aeruginosa (Pseudomon
as aeruginosa) (10 5 cells / ml) was dropped onto each of 15 ml and cultured at 37 ° C for 18 hours. The bacterial solution was washed away with physiological saline, and the number of Escherichia coli and the number of Pseudomonas aeruginosa present in this solution were measured. Table 2 shows the results. Generally, when the number of bacteria after 18 hours is reduced by two digits or more due to the added initial number of bacteria, it can be said that the bacteria have antibacterial properties.
試験例３（抗菌剤スプレーの抗菌性試験） 実施例３で作成した抗菌剤スプレーについて抗菌性試
験を行なった。供試品として同じスプレーを綿布に３秒
間噴霧し、それを60℃にて１時間乾燥したものを用意し
た。以下の操作は試験例２と同様で行った。結果を表４
に示す。Test Example 3 (Antibacterial test of antibacterial agent spray) An antibacterial test was performed on the antibacterial agent spray prepared in Example 3. As a test sample, the same spray was sprayed on a cotton cloth for 3 seconds and dried at 60 ° C. for 1 hour. The following operation was performed in the same manner as in Test Example 2. Table 4 shows the results
Shown in
試験例４（抗菌剤軟膏の抗菌性試験） 実施例４で作成した抗菌剤軟膏について抗菌性試験を
行なった。供試品として同軟膏を綿布に10g/m2塗布した
ものを用意した。以下の操作は試験例２と同様で行っ
た。結果を表５に示す。 Test Example 4 (Antimicrobial test of antimicrobial ointment) An antimicrobial test was performed on the antimicrobial ointment prepared in Example 4. As a test product, a product obtained by applying the same ointment to a cotton cloth at 10 g / m 2 was prepared. The following operation was performed in the same manner as in Test Example 2. Table 5 shows the results.
実施例５ 種々の濃度の硝酸銀及びリン酸水素二ナトリウムの水
溶液について、銀及びリンの溶解濃度（溶出量）並びに
大腸菌に対する抗菌力を求めた。結果を表６に示す。表
６の結果から、水溶性銀塩である硝酸銀を用いた系で
は、リン酸水素二ナトリウムを共存させることにより、
約5000分の１の銀塩の添加量で同等の効果が得られた
（No.7とNo.13との比較）。又、リン酸塩の共存下で
は、銀の溶出量が0.053ppm（No.8参照）から抗菌性が現
われた。 Example 5 With respect to aqueous solutions of silver nitrate and disodium hydrogen phosphate at various concentrations, the dissolution concentrations (elution amounts) of silver and phosphorus and the antibacterial activity against Escherichia coli were determined. Table 6 shows the results. From the results in Table 6, in the system using silver nitrate, which is a water-soluble silver salt, by allowing disodium hydrogen phosphate to coexist,
The same effect was obtained with an addition amount of about 1/5000 of the silver salt (comparison between No. 7 and No. 13). In addition, in the presence of phosphate, antimicrobial properties appeared when the amount of silver eluted was 0.053 ppm (see No. 8).
実施例６ 種々の濃度の銀担持（10％）ゼオライト及びリン酸ナ
トリウムのフェニルアルコール溶液について、銀及びリ
ンの溶解濃度（溶出量）並びに大腸菌に対する抗菌力を
求めた。結果を表７に示す。表７の結果から、銀イオン
及びリン酸イオンの溶解性の低いフェニルアルコールを
用いた系でも、リン酸ナトリウムを共存させることによ
り約1000分の１の銀塩添加量で同等の効果が得られた
（No.3とNo.15との比較）。又リン酸塩の共存下では、
銀の溶出量が0.070〜0.10ppm（No.8、11参照）から抗菌
性が現われた。さらに、リン酸塩の銀に対する比率は1:
1（No.12）〜1:50（No.9）の範囲で効果的であることが
わかる。 Example 6 With respect to phenyl alcohol solutions of various concentrations of silver-supported (10%) zeolite and sodium phosphate, the dissolution concentrations of silver and phosphorus (elution amount) and antibacterial activity against Escherichia coli were determined. Table 7 shows the results. From the results shown in Table 7, even in a system using phenyl alcohol having low solubility of silver ions and phosphate ions, the same effect can be obtained by adding sodium phosphate in an amount of about 1/1000 of the silver salt. (Comparison between No.3 and No.15). In the presence of phosphate,
The antibacterial properties appeared when the amount of silver eluted was 0.070 to 0.10 ppm (see Nos. 8 and 11). In addition, the ratio of phosphate to silver is 1:
It is understood that the effect is effective in the range of 1 (No. 12) to 1:50 (No. 9).
実施例７ 種々の濃度の塩化銀及びリン酸カルシウムの水溶液に
ついて、銀及びリンの溶解濃度（溶出量）並びに大腸菌
に対する抗菌力を求めた。結果を表８に示す。表８の結
果から、水不溶性銀塩である塩化銀を用いた系では、リ
ン酸カルシウムを共存させることにより、約100000分の
１の銀塩添加量で同等の効果が得られた（No.7とNo.12
との比較）。又、リン酸塩の共存下では、銀の溶出量が
0.068ppm（No.8参照）から、抗菌性が現われた。 Example 7 With respect to aqueous solutions of silver chloride and calcium phosphate having various concentrations, the dissolution concentrations (elution amounts) of silver and phosphorus and the antibacterial activity against Escherichia coli were determined. Table 8 shows the results. From the results shown in Table 8, in the system using silver chloride, which is a water-insoluble silver salt, the same effect was obtained with the addition of about 1 / 100,000 silver salt by coexisting calcium phosphate (No. 7 and No. 7). No.12
And comparison). Also, in the presence of phosphate, the amount of silver eluted
From 0.068 ppm (see No. 8), antibacterial properties appeared.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 栗原 靖夫 愛知県名古屋市瑞穂区豊岡通３丁目35番 地 (56)参考文献 特開 昭63−88109（ＪＰ，Ａ) 特開 昭63−170497（ＪＰ，Ａ) 特開 昭59−231006（ＪＰ，Ａ) 特開 平２−96508（ＪＰ，Ａ) 特開 平４−503018（ＪＰ，Ａ) 特開 平２−306904（ＪＰ，Ａ) (58)調査した分野(Int.Cl.6，ＤＢ名) A01N 59/16 A01N 59/26 ＣＡ（ＳＴＮ) ＲＥＧＩＳＴＲＹ（ＳＴＮ) ＷＰＩＤＳ（ＳＴＮ)──────────────────────────────────────────────────続 き Continuation of front page (72) Inventor Yasuo Kurihara 3-35, Toyooka-dori, Mizuho-ku, Nagoya-shi, Aichi (56) References JP-A-63-88109 (JP, A) JP-A-63-170497 ( JP, A) JP-A-59-231006 (JP, A) JP-A-2-96508 (JP, A) JP-A-4-503018 (JP, A) JP-A-2-306904 (JP, A) (58) 6) Fields surveyed (Int. Cl. 6 , DB name) A01N 59/16 A01N 59/26 CA (STN) REGISTRY (STN) WPIDS (STN)
Claims (2)
あって、ジアミン銀硝酸塩、ジアミン銀硫酸塩、銀担持
ゼオライト、及び銀担持無定形アルミノケイ酸塩からな
る群から選ばれる少なくとも１種の銀含有化合物と、メ
タリン酸、リン酸ナトリウム、リン酸カリウム、リン酸
アンモニウム、リン酸水素ナトリウム、リン酸水素二ナ
トリウム、トリポリリン酸ナトリウム、リン酸トリフェ
ニル、Ｄ−フルクトース−６−リン酸、及びホスファチ
ジルコリンからなる群から選ばれる少なくとも１種のリ
ン含有化合物を共存させることを特徴とする方法。1. A method for improving the antibacterial property of a silver-containing compound, comprising at least one selected from the group consisting of diamine silver nitrate, diamine silver sulfate, silver-supported zeolite, and silver-supported amorphous aluminosilicate. A silver-containing compound, metaphosphate, sodium phosphate, potassium phosphate, ammonium phosphate, sodium hydrogenphosphate, disodium hydrogenphosphate, sodium tripolyphosphate, triphenyl phosphate, D-fructose-6-phosphate, and A method comprising coexisting at least one phosphorus-containing compound selected from the group consisting of phosphatidylcholines.
担持ゼオライト、及び銀担持無定形アルミノケイ酸塩か
らなる群から選ばれる少なくとも１種の銀含有化合物
と、メタリン酸、リン酸ナトリウム、リン酸カリウム、
リン酸アンモニウム、リン酸水素ナトリウム、リン酸水
素二ナトリウム、トリポリリン酸ナトリウム、リン酸ト
リフェニル、Ｄ−フルクトース−６−リン酸、及びホス
ファチジルコリンからなる群から選ばれる少なくとも１
種のリン含有化合物を含有する抗菌剤。2. At least one silver-containing compound selected from the group consisting of diamine silver nitrate, diamine silver sulfate, silver-supported zeolite, and silver-supported amorphous aluminosilicate, metaphosphoric acid, sodium phosphate, phosphoric acid potassium,
At least one selected from the group consisting of ammonium phosphate, sodium hydrogen phosphate, disodium hydrogen phosphate, sodium tripolyphosphate, triphenyl phosphate, D-fructose-6-phosphate, and phosphatidylcholine
An antimicrobial agent containing various phosphorus-containing compounds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17139189A JP2847529B2 (en) | 1989-07-03 | 1989-07-03 | Method for improving antibacterial property of silver-containing compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17139189A JP2847529B2 (en) | 1989-07-03 | 1989-07-03 | Method for improving antibacterial property of silver-containing compound |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0338504A JPH0338504A (en) | 1991-02-19 |
| JP2847529B2 true JP2847529B2 (en) | 1999-01-20 |
Family
ID=15922294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17139189A Expired - Lifetime JP2847529B2 (en) | 1989-07-03 | 1989-07-03 | Method for improving antibacterial property of silver-containing compound |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2847529B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010103682A1 (en) | 2009-03-10 | 2010-09-16 | 株式会社カネカ | Antibacterial agent and method for using same |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2654694B2 (en) * | 1989-09-13 | 1997-09-17 | 日本ポリウレタン工業株式会社 | Method for producing thermoplastic polyurethane resin with excellent thermal stability |
| JP2734852B2 (en) * | 1991-12-27 | 1998-04-02 | 東亞合成株式会社 | Water absorbent resin |
| JP2599645Y2 (en) * | 1992-06-12 | 1999-09-13 | アイリスオーヤマ株式会社 | Pet toilet |
| GB9424562D0 (en) * | 1994-12-06 | 1995-01-25 | Giltech Ltd | Product |
| JP3440606B2 (en) * | 1995-02-03 | 2003-08-25 | 東亞合成株式会社 | Antibacterial ABS resin composition |
| JP4143206B2 (en) | 1998-03-09 | 2008-09-03 | 明晃化成工業株式会社 | Storage case for disk for recording media |
| JP5182647B2 (en) * | 2009-02-13 | 2013-04-17 | 三菱マテリアル株式会社 | Antibacterial material |
| JP5568942B2 (en) * | 2009-10-09 | 2014-08-13 | 三菱マテリアル株式会社 | Antibacterial material |
| KR101830452B1 (en) * | 2010-03-15 | 2018-02-20 | 도아고세이가부시키가이샤 | Antibacterial processing agent for water treatment, method for producing antibacterial processing agent for water treatment, and water treatment method |
-
1989
- 1989-07-03 JP JP17139189A patent/JP2847529B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010103682A1 (en) | 2009-03-10 | 2010-09-16 | 株式会社カネカ | Antibacterial agent and method for using same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0338504A (en) | 1991-02-19 |
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