JP2023546265A - 全自動剥離細胞標本作製方法 - Google Patents
全自動剥離細胞標本作製方法 Download PDFInfo
- Publication number
- JP2023546265A JP2023546265A JP2023546374A JP2023546374A JP2023546265A JP 2023546265 A JP2023546265 A JP 2023546265A JP 2023546374 A JP2023546374 A JP 2023546374A JP 2023546374 A JP2023546374 A JP 2023546374A JP 2023546265 A JP2023546265 A JP 2023546265A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- exfoliated
- cell
- specimen preparation
- cell specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 49
- 238000004062 sedimentation Methods 0.000 claims abstract description 45
- 238000010186 staining Methods 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 11
- 230000002452 interceptive effect Effects 0.000 claims abstract description 5
- 239000000126 substance Substances 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 114
- 239000012120 mounting media Substances 0.000 claims description 48
- 239000012528 membrane Substances 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 25
- 238000005406 washing Methods 0.000 claims description 21
- 230000000762 glandular Effects 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 11
- 210000004085 squamous epithelial cell Anatomy 0.000 claims description 11
- 239000000872 buffer Substances 0.000 claims description 8
- 210000003743 erythrocyte Anatomy 0.000 claims description 8
- 238000011085 pressure filtration Methods 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 210000000265 leukocyte Anatomy 0.000 claims description 6
- 239000003761 preservation solution Substances 0.000 claims description 6
- 239000000080 wetting agent Substances 0.000 claims description 6
- 210000004698 lymphocyte Anatomy 0.000 claims description 5
- 239000004925 Acrylic resin Substances 0.000 claims description 4
- 229920000178 Acrylic resin Polymers 0.000 claims description 4
- 239000002861 polymer material Substances 0.000 claims description 4
- 239000003172 expectorant agent Substances 0.000 claims description 3
- 229940066491 mucolytics Drugs 0.000 claims description 3
- 239000006174 pH buffer Substances 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims description 2
- 239000000178 monomer Substances 0.000 claims description 2
- 239000008393 encapsulating agent Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 238000004806 packaging method and process Methods 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 abstract description 3
- 238000002347 injection Methods 0.000 description 35
- 239000007924 injection Substances 0.000 description 35
- 230000007246 mechanism Effects 0.000 description 23
- 238000004140 cleaning Methods 0.000 description 22
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- 239000000463 material Substances 0.000 description 15
- 239000000243 solution Substances 0.000 description 14
- 239000007853 buffer solution Substances 0.000 description 13
- 238000005538 encapsulation Methods 0.000 description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 238000003860 storage Methods 0.000 description 8
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- 238000010330 laser marking Methods 0.000 description 6
- 229920006254 polymer film Polymers 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 238000010884 ion-beam technique Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000008096 xylene Substances 0.000 description 4
- 239000004471 Glycine Substances 0.000 description 3
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 101150038956 cup-4 gene Proteins 0.000 description 3
- 125000004122 cyclic group Chemical group 0.000 description 3
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 3
- 238000001125 extrusion Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- ZDQNWDNMNKSMHI-UHFFFAOYSA-N 1-[2-(2-prop-2-enoyloxypropoxy)propoxy]propan-2-yl prop-2-enoate Chemical compound C=CC(=O)OC(C)COC(C)COCC(C)OC(=O)C=C ZDQNWDNMNKSMHI-UHFFFAOYSA-N 0.000 description 2
- VOBUAPTXJKMNCT-UHFFFAOYSA-N 1-prop-2-enoyloxyhexyl prop-2-enoate Chemical compound CCCCCC(OC(=O)C=C)OC(=O)C=C VOBUAPTXJKMNCT-UHFFFAOYSA-N 0.000 description 2
- DAKWPKUUDNSNPN-UHFFFAOYSA-N Trimethylolpropane triacrylate Chemical compound C=CC(=O)OCC(CC)(COC(=O)C=C)COC(=O)C=C DAKWPKUUDNSNPN-UHFFFAOYSA-N 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000003486 chemical etching Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000005530 etching Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000007723 transport mechanism Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00039—Transport arrangements specific to flat sample substrates, e.g. pusher blade
- G01N2035/00049—Transport arrangements specific to flat sample substrates, e.g. pusher blade for loading/unloading a carousel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00089—Magazines
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00138—Slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0403—Sample carriers with closing or sealing means
- G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0437—Cleaning cuvettes or reaction vessels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
- G01N2035/0441—Rotary sample carriers, i.e. carousels for samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
剥離細胞の前処理ステップS1であって、標本作製が必要な剥離細胞に自動前処理を行って妨害物質を除去する、ステップS1と、
ステップS1で前処理した剥離細胞を循環式沈降モジュールに自動的に移して恒温沈降を行い、それらを染色及び保湿するステップS2と、
ステップS2の保湿後の剥離細胞を自動的に液体封入するステップS3とを含む。
細胞洗浄チャンバを使用して細胞を洗浄及び濾過するステップS11であって、細胞洗浄チャンバの底部には、貫通孔直径が5~20μmで貫通孔サイズが均一なフィルタ膜が含まれており、フィルタ膜は、フィルタ膜の表面に扁平上皮細胞及び腺細胞を捕捉しながら、赤血球、白血球、リンパ球、及び細胞保存液を選択的に通過させる、ステップS11と、
フィルタ膜に捕捉された扁平上皮細胞及び腺細胞を第1の緩衝液で洗浄して再懸濁し、フィルタ膜に捕捉された扁平上皮細胞及び腺細胞を得るステップS12とを含む。
質量百分率50%~80%のUVLED光硬化性アクリル樹脂、
質量百分率5%~40%の光硬化性アクリレートモノマー、
質量百分率0.5%~8%の反応性アミンアクリル樹脂、
質量百分率2%~8%の光開始剤、
質量百分率0.05%~0.5%のレベリング剤、
及び質量百分率0.1%~0.5%の湿潤剤を含む。
1、本発明の発明者は、初めて循環式沈降モジュールと自動封入を組み合わせるため、本発明の全自動剥離細胞標本作製方法は全自動化生産を実現することができ、特に、本発明の循環式沈降モジュールは標本作製と染色を同時に行うことができ、チャンバ自体は一度の使用ではなく繰り返し使用することができる。したがって、本発明は循環利用率が高い。
2、本発明の全自動剥離細胞標本作製方法は、自動標本作製、染色及び封入を実現し、無人のハイスループット生産、高品質の標本作製及び封入を実現する。
3、本発明は、液体封入のためにキシレン以外の新型高分子材料を使用する。この新型高分子材料を細胞層の表面にスプレーし、紫外線を照射するとこの新型高分子材料は急速に硬化し、硬化後に形成される硬化層は完全に透明であり、調製された細胞サンプルの形態をよりよく示すことができ、またこの高分子材料は人体に無害であり、硬化プロセスは数秒しかかからない。
剥離細胞の前処理ステップS1であって、標本作製が必要な剥離細胞に自動前処理を行って妨害物質を除去する、ステップS1と、
ステップS1で前処理した剥離細胞を循環式沈降モジュールに自動的に移して恒温沈降を行い、それらを染色及び保湿するステップS2と、
ステップS2の保湿後の剥離細胞を自動的に液体封入するステップS3とを含む。
細胞洗浄チャンバを使用して細胞を洗浄及び濾過するステップS11であって、細胞洗浄チャンバの底部には、貫通孔直径が5~20μmで貫通孔サイズが均一なフィルタ膜が含まれており、フィルタ膜は、フィルタ膜の表面に扁平上皮細胞及び腺細胞を捕捉しながら、赤血球、白血球、リンパ球、及び細胞保存液を選択的に通過させる、ステップS11と、
フィルタ膜に捕捉された扁平上皮細胞及び腺細胞を第1の緩衝液で洗浄して再懸濁し、フィルタ膜に捕捉された扁平上皮細胞及び腺細胞を得るステップS12とを含む。
Claims (10)
- 全自動剥離細胞標本作製方法であって、
剥離細胞の前処理ステップS1であって、標本作製が必要な前記剥離細胞に自動前処理を行って妨害物質を除去する、ステップS1と、
前記ステップS1で前記前処理した前記剥離細胞を循環式沈降モジュールに自動的に移して恒温沈降を行い、それらを染色及び保湿するステップS2と、
前記ステップS2の前記保湿後の前記剥離細胞を自動的に液体封入するステップS3と、を含む、
ことを特徴とする全自動剥離細胞標本作製方法。 - 前記ステップS1において、前記剥離細胞の前処理は、
細胞洗浄チャンバを使用して細胞を洗浄及び濾過するステップS11であって、前記細胞洗浄チャンバの底部には、貫通孔直径が5μm~20μmで貫通孔サイズが均一なフィルタ膜が含まれており、前記フィルタ膜は、前記フィルタ膜の表面に扁平上皮細胞及び腺細胞を捕捉しながら、赤血球、白血球、リンパ球、及び細胞保存液を選択的に通過させる、ステップS11と、
前記フィルタ膜に捕捉された扁平上皮細胞及び腺細胞を第1の緩衝液で洗浄して再懸濁し、前記フィルタ膜に捕捉された扁平上皮細胞及び腺細胞を得るステップS12と、を含む、
ことを特徴とする請求項1に記載の剥離細胞標本作製方法。 - 前記ステップS11において、濾過に陽圧濾過を使用し、使用される前記陽圧濾過の圧力は50Pa~1000Paである、
ことを特徴とする請求項2に記載の剥離細胞標本作製方法。 - 前記ステップS12において、前記第1の緩衝液は、pH緩衝剤、界面活性剤、粘液溶解剤、及びタンパク質保護剤を含む、
ことを特徴とする請求項2に記載の剥離細胞標本作製方法。 - 前記ステップS11において、前記細胞洗浄チャンバ構造は傾斜面上に設けられ、前記傾斜面と水平面の間に傾斜夾角がある、
ことを特徴とする請求項2に記載の剥離細胞標本作製方法。 - 前記傾斜夾角が5°~30°である、
ことを特徴とする請求項5に記載の剥離細胞標本作製方法。 - 前記ステップS2において、前処理した前記剥離細胞をロボットアームによって循環式沈降モジュールに移す、
ことを特徴とする請求項1に記載の剥離細胞標本作製方法。 - 前記ステップS2において、恒温制御システムによって恒温を制御し、前記恒温制御システムは加熱素子及び前記加熱素子に接続された温度コントローラを含み、恒温温度の調整範囲は25℃~45℃である、
ことを特徴とする請求項1に記載の剥離細胞標本作製方法。 - 前記ステップS2において、保湿ステップは、湿潤剤0.2ml~0.3mlを加え、3秒~8秒後にそれを吸い取り、細胞層の表面に湿潤層を残すことを含む、
ことを特徴とする請求項1に記載の剥離細胞標本作製方法。 - 前記ステップS3において、封入に高分子材料液体封入剤を使用し、
前記高分子材料液体封入剤は、
質量百分率50%~80%のUVLED光硬化性アクリル樹脂、
質量百分率5%~40%の光硬化性アクリレートモノマー、
質量百分率0.5%~8%の反応性アミンアクリル樹脂、
質量百分率2%~8%の光開始剤、
質量百分率0.05%~0.5%のレベリング剤、
及び質量百分率0.1%~0.5%の湿潤剤を含む、
ことを特徴とする請求項1に記載の剥離細胞標本作製方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011074112.3A CN112113817B (zh) | 2020-10-09 | 2020-10-09 | 一种全自动的脱落细胞制片方法 |
CN202011074112.3 | 2020-10-09 | ||
PCT/CN2021/088915 WO2022073335A1 (zh) | 2020-10-09 | 2021-04-22 | 一种全自动的脱落细胞制片方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2023546265A true JP2023546265A (ja) | 2023-11-01 |
Family
ID=73798354
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2023546374A Pending JP2023546265A (ja) | 2020-10-09 | 2021-04-22 | 全自動剥離細胞標本作製方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US20230366790A1 (ja) |
EP (1) | EP4206642A4 (ja) |
JP (1) | JP2023546265A (ja) |
CN (1) | CN112113817B (ja) |
WO (1) | WO2022073335A1 (ja) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112113818A (zh) * | 2020-10-09 | 2020-12-22 | 嘉兴晶铸生物科技有限公司 | 一种恒温全自动化细胞制片一体化装置及制片方法 |
CN112113817B (zh) * | 2020-10-09 | 2023-03-14 | 嘉兴晶铸生物科技有限公司 | 一种全自动的脱落细胞制片方法 |
CN112881120B (zh) * | 2021-01-14 | 2024-01-26 | 杭州海世嘉生物科技有限公司 | 一种用于细胞制片的辅助装置及离心方法 |
CN117606885B (zh) * | 2024-01-24 | 2024-03-26 | 成都川哈工机器人及智能装备产业技术研究院有限公司 | 一种智能化病理成片***及方法 |
CN117782746B (zh) * | 2024-02-27 | 2024-05-07 | 江苏成升基因精准医疗科技有限公司 | 一种载玻片染色、封片一体机 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4992365A (en) * | 1984-04-23 | 1991-02-12 | Hyman Edward S | Method of detecting bacteria in urine |
CN201255707Y (zh) * | 2008-04-23 | 2009-06-10 | 珠海市丽拓发展有限公司 | 一种细胞制片染色装置 |
CN201225970Y (zh) * | 2008-04-29 | 2009-04-22 | 珠海市丽拓发展有限公司 | 一种细胞制片染色机 |
CN201788111U (zh) * | 2010-09-19 | 2011-04-06 | 王珑 | Lct液基薄层细胞和细胞dna快速制片与染色一体机 |
CN101957279A (zh) * | 2010-09-19 | 2011-01-26 | 王珑 | 全自动lct液基薄层细胞和细胞dna快速制片与染色一体机 |
US20140127745A1 (en) * | 2011-06-24 | 2014-05-08 | Biotechnology Developers, S.A. | Method, compositions and device for preparing cytological specimens |
CN102732094B (zh) * | 2012-05-21 | 2014-04-09 | 史海生 | Led-uv面光源光固化丝网印刷油墨组合物及其制法 |
CN102759474A (zh) * | 2012-06-13 | 2012-10-31 | 长沙康柏恩医疗科技有限公司 | 运用注射移液原理的液基细胞制片染色机 |
US9513224B2 (en) * | 2013-02-18 | 2016-12-06 | Theranos, Inc. | Image analysis and measurement of biological samples |
CN106018033B (zh) | 2016-07-04 | 2019-06-11 | 邱加伟 | 全自动液基薄层细胞制片染色装置 |
CN206974785U (zh) * | 2017-07-26 | 2018-02-06 | 湖南品胜生物技术有限公司 | 一种用于薄层细胞学检测技术的全自动制片机 |
CN108535068A (zh) * | 2018-04-04 | 2018-09-14 | 南京福怡科技发展股份有限公司 | 一种细胞学dna检测样本保存液预处理装置 |
KR102103141B1 (ko) * | 2018-09-08 | 2020-04-21 | (주)바이오다인 | 탈락세포 보관 및 도말작업을 위한 바이알 장치 |
CN110398401A (zh) * | 2019-07-12 | 2019-11-01 | 上海晶铸生物科技有限公司 | 一种液基薄层细胞制片的方法 |
CN111175099B (zh) * | 2019-12-09 | 2024-06-25 | 湖北泰康医疗设备有限公司 | 一种用于检查膀胱癌的体液细胞的提取和制片方法 |
CN110940568B (zh) * | 2019-12-17 | 2022-02-18 | 武汉友芝友医疗科技股份有限公司 | 一种用于细胞富集、分离、染色、制片的一体化*** |
CN111060368A (zh) * | 2019-12-18 | 2020-04-24 | 苏州浚惠生物科技有限公司 | 基于液基细胞制片的单细胞制片方法 |
CN111057446A (zh) * | 2019-12-31 | 2020-04-24 | 嘉兴晶铸生物科技有限公司 | 一种uvled固化载玻片封固方法 |
CN112113817B (zh) * | 2020-10-09 | 2023-03-14 | 嘉兴晶铸生物科技有限公司 | 一种全自动的脱落细胞制片方法 |
-
2020
- 2020-10-09 CN CN202011074112.3A patent/CN112113817B/zh active Active
-
2021
- 2021-04-22 WO PCT/CN2021/088915 patent/WO2022073335A1/zh unknown
- 2021-04-22 EP EP21876858.8A patent/EP4206642A4/en active Pending
- 2021-04-22 JP JP2023546374A patent/JP2023546265A/ja active Pending
- 2021-04-22 US US18/029,674 patent/US20230366790A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN112113817A (zh) | 2020-12-22 |
US20230366790A1 (en) | 2023-11-16 |
EP4206642A4 (en) | 2024-03-13 |
EP4206642A1 (en) | 2023-07-05 |
CN112113817B (zh) | 2023-03-14 |
WO2022073335A1 (zh) | 2022-04-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2023546265A (ja) | 全自動剥離細胞標本作製方法 | |
US5282978A (en) | Specimen processor method and apparatus | |
WO2022073332A1 (zh) | 一种恒温全自动化细胞制片一体化装置及制片方法 | |
CN109963652B (zh) | 用于提取样品中包含的微生物的提取***和方法 | |
CN205538409U (zh) | 一种染色装置及采取该染色装置的染片机 | |
AU2020417834B2 (en) | Automated staining system and reaction chamber | |
US20220026321A1 (en) | Specimen processing systems, pipette assemblies and methods for preparing reagents | |
CA2981975A1 (en) | Automated specimen deposition systems and associated methods | |
CN112881738A (zh) | 一种全自动生物样品分析一体化设备及控制方法 | |
CN209166961U (zh) | 一种切片制作染色与扫描一体机 | |
CN216838008U (zh) | 一种细胞制备*** | |
CN113567422B (zh) | 一种便携式化学发光检测仪及其控制方法 | |
CN109916817A (zh) | 一种移液及分析装置 | |
CN215179058U (zh) | 一种存放板式自动封片机 | |
CN112730867A (zh) | 一种流式分析仪的样本制备装置及制备方法 | |
TW202141041A (zh) | 液態檢體的自動化處理裝置 | |
JPH0871531A (ja) | 免疫測定装置 | |
CN213842795U (zh) | 一种恒温全自动化细胞制片一体化装置 | |
CN221351223U (zh) | 一种用于革兰染色***分泌物检测的一体机 | |
CN215448677U (zh) | 一种全自动采血管标本处理用离心装置 | |
CN215866722U (zh) | 一种流式分析仪的样本制备装置 | |
JPH01141356A (ja) | 糖類の蛍光標識方法 | |
CN217786685U (zh) | 一种细胞因子的样本制备装置 | |
CN221445583U (zh) | 一种密闭式全自动免疫组化染色仪 | |
CN219475627U (zh) | Ctc自动检测仪 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20230407 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20240126 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20240305 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20240520 |
|
RD01 | Notification of change of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7421 Effective date: 20240520 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20240520 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20240619 |