JP2018112354A - Freezing cold insulation container - Google Patents
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本発明は、試料を凍結し且つ保冷するための凍結保冷器に関し、特に生物学的試料を凍結し且つ保冷するための凍結保冷器に関する。 The present invention relates to a cryocooler for freezing and refrigeration of samples, and more particularly to a cryocooler for freezing and refrigeration of biological samples.
従来から、血液、尿及び便等の生物学的試料を輸送する場合、試料中のタンパク質や糖等の種々の成分が分解しないように、低温環境下に試料を置いて輸送する。例えば、生物学的試料は、発泡スチロールからなる容器内に保冷剤やドライアイスと共に入れられて輸送される。このような輸送手段以外に、低温輸送の必要がある試料のための専用の輸送容器が、例えば特許文献１及び２に開示されている。 Conventionally, when transporting biological samples such as blood, urine, and stool, the samples are transported in a low-temperature environment so that various components such as proteins and sugars in the sample are not degraded. For example, a biological sample is transported by being placed in a container made of expanded polystyrene together with a cooling agent and dry ice. In addition to such transport means, for example, Patent Documents 1 and 2 disclose dedicated transport containers for samples that need to be transported at a low temperature.
特許文献１の輸送容器は、断熱性の容器本体と、該容器本体の開口部を塞ぐ蓋部材と、該容器本体に収容された０℃〜５℃の温度範囲でゲル状である保冷剤と、該保冷剤の上方に配置されて且つ試料が収容された試料容器が挿入可能な孔を有する保持プレートとを備えている。特許文献２の輸送容器は、断熱構造の外箱と、該外箱に着脱可能に収容されて且つ保冷剤を収容する内箱とを備え、該内箱には試料が収容された試料容器を保持するための保持部材が設けられている。 The transport container of Patent Document 1 includes a heat-insulating container body, a lid member that closes the opening of the container body, and a cryogen that is gel-like in a temperature range of 0 ° C. to 5 ° C. accommodated in the container body. And a holding plate having a hole which is disposed above the cryogen and into which a sample container in which a sample is accommodated can be inserted. The transport container of Patent Document 2 includes an outer box having a heat insulating structure, and an inner box that is detachably accommodated in the outer box and contains a cooling agent, and a sample container in which a sample is accommodated is provided in the inner box. A holding member for holding is provided.
上記特許文献１及び２に開示される輸送容器によると、当該容器を予め冷却しておくことでその容器に保冷機能を付与することができ、試料が入った試料容器を輸送容器内で保持できる。このため、当該輸送容器を用いることで簡便且つ安定的に試料を保冷した状態で輸送することができる。 According to the transport container disclosed in Patent Documents 1 and 2, the container can be cooled in advance, so that the container can be provided with a cooling function, and the sample container containing the sample can be held in the transport container. . For this reason, by using the said transport container, it can be transported in the state which kept the sample cold simply and stably.
しかしながら、特許文献１の輸送容器では、使用前に輸送容器全体を冷蔵庫又は冷凍庫に入れる必要があり、冷蔵庫又は冷凍庫内が嵩張ることとなる。また、特許文献１では、０℃〜５℃の温度範囲でゲル状である保冷剤が輸送容器内に充填されており、該保冷剤が試料容器に密着するように変形するが、保冷剤を凍結させると保冷剤が変形できずに試料容器を保冷剤内に挿入することができない。従って、保冷剤を凍結させる場合は、試料容器を予め保冷剤内に挿入した状態で輸送容器を冷凍庫に入れて保冷剤を凍結させる必要がある。 However, in the transport container of Patent Document 1, it is necessary to put the entire transport container in a refrigerator or freezer before use, and the inside of the refrigerator or freezer becomes bulky. Moreover, in patent document 1, the cryogenic agent which is a gel form in the temperature range of 0 degreeC-5 degreeC is filled in the transport container, and it deform | transforms so that this cryogen may closely_contact | adhere to a sample container, When frozen, the cryogen cannot be deformed and the sample container cannot be inserted into the cryogen. Therefore, when freezing the cryogen, it is necessary to freeze the cryogen by placing the transport container in a freezer with the sample container inserted in the cryogen beforehand.
近年、自己の生物学的試料を自宅で採取し、試験所に郵送することにより健康状態を検査する在宅検診が行われているが、上記特許文献１に開示の輸送容器を用いる場合、自宅の冷凍庫に試料と共に輸送容器を入れなければならない。しかし、生物学的試料が便や尿である場合、多くの人にとって自宅の冷凍庫にそのような試料を入れることに抵抗がある。なお、特許文献１には、この問題を解決するために、保冷剤を冷凍庫で凍結させる際に、試料容器の代わりにダミー容器をスペーサーとして保冷剤に挿入しておくことにより、凍結後の保冷剤に試料容器を挿入できる空間を形成する旨が記載されている。しかしながら、この方法では、ダミー容器を準備する必要があり、また、保冷剤の凍結後にダミー容器と試料容器とを入れ替える工程が必要となる。さらに、特許文献１に開示の輸送容器は保冷剤が密封されない構造のため、保冷剤の漏洩、汚染の恐れがあり、在宅検診等で不特定多数のユーザが使用するには適さない。 In recent years, home medical examinations have been performed in which a biological sample is collected at home and mailed to a laboratory to check the health condition. When the transport container disclosed in Patent Document 1 is used, The transport container must be placed with the sample in the freezer. However, if the biological sample is stool or urine, many people are reluctant to put such sample in their home freezer. In Patent Document 1, in order to solve this problem, when the cryogen is frozen in a freezer, a dummy container is inserted into the cryogen as a spacer instead of the sample container, so that the cryogen after freezing is inserted. It describes that a space for inserting a sample container into the agent is formed. However, in this method, it is necessary to prepare a dummy container, and a process of exchanging the dummy container and the sample container after the freezing agent is frozen is necessary. Furthermore, since the transport container disclosed in Patent Document 1 has a structure in which the coolant is not sealed, there is a risk of leakage of the coolant and contamination, and it is not suitable for use by an unspecified number of users at home examinations.
一方、特許文献２の輸送容器は、内箱に保冷剤が収容されており、試料容器が保冷剤に直接接触することがないため、保冷剤が凍結しても容器内への試料の挿入に支障がない。また、保冷剤を含む内箱が外箱に対して着脱可能であるため、内箱のみを予め冷凍庫に入れておき、使用前に取り出して内箱に試料を挿入し、内箱を外箱に収容して使用することができる。しかしながら、特許文献２の輸送容器は、試料を凍結しないように０℃付近で保管することを目的とするものであり、過剰に冷却された保冷剤を０℃付近にまで加温する手段を備えるため、特許文献２の輸送容器を用いて試料を凍結させることはできない。従って、特許文献２の輸送容器を用いる場合であっても、試料を凍結させるためには試料自体を冷凍庫に入れる必要がある。そうすると、やはり、生物学的試料が特に便や尿である場合に多くの人に不快感を与えることとなる。 On the other hand, since the transport container of Patent Document 2 contains a cryogen in the inner box and the sample container does not come into direct contact with the cryogen, the sample can be inserted into the container even if the cryogen is frozen. There is no hindrance. Also, since the inner box containing the cryogen can be attached to and detached from the outer box, only the inner box is put in the freezer beforehand, removed before use, the sample is inserted into the inner box, and the inner box is put into the outer box. Can be housed and used. However, the transport container of Patent Document 2 is intended to store the sample at around 0 ° C. so as not to freeze, and includes means for heating an excessively cooled cryogen to around 0 ° C. Therefore, the sample cannot be frozen using the transport container of Patent Document 2. Therefore, even when the transport container of Patent Document 2 is used, it is necessary to put the sample itself in a freezer in order to freeze the sample. This again causes discomfort to many people, especially when the biological sample is stool or urine.
本発明は、前記の問題に鑑みてなされたものであり、その目的は、生物学的試料を凍結できると共に、冷凍状態で長期間保管することができる凍結保冷器を得ることにある。 The present invention has been made in view of the above problems, and an object of the present invention is to obtain a cryocooler that can freeze a biological sample and store it in a frozen state for a long period of time.
前記の目的を達成するために、本発明では、断熱構造の容器本体内に、保冷剤を含む冷却部材を着脱可能に収容できるようにし、その保冷剤には融点が−１０℃以下のものを用いるようにした。 In order to achieve the above object, in the present invention, a cooling member containing a cooling agent can be detachably accommodated in a container body having a heat insulating structure, and the cooling agent has a melting point of −10 ° C. or lower. I used it.
具体的に、本発明に係る凍結保冷器は、生物学的試料を凍結させて保冷するための凍結保冷器であって、断熱構造の容器本体と、容器本体に着脱可能に収容され、内部に保冷剤を含む冷却部材と、容器本体の開口を塞ぐ蓋部材とを備え、冷却部材は、生物学的試料を含む試料容器を収容するための収容部を有し、保冷剤は、融点が−１０℃以下であることを特徴とする。 Specifically, a cryocooler according to the present invention is a cryocooler for freezing and keeping a biological sample, and is detachably accommodated in a heat insulating container body and the container body. A cooling member containing a cryogen, and a lid member that closes the opening of the container body, the cooling member has an accommodating portion for accommodating a sample container containing a biological sample, and the cryogen has a melting point of − It is 10 degrees C or less.
本発明に係る凍結保冷器によると、融点が−１０℃以下である保冷剤を含む冷却部材を用いているため、容器本体内の温度を−１０℃以下にすることができ、血液や尿や便といった生物学的試料を凍結することができる。また、容器本体は断熱構造であるため、保冷効果が高く、生物学的試料を凍結状態で長期間保冷することができる。さらに、冷却部材は、容器本体に着脱可能に収容できるため、予め冷却部材のみを冷凍庫に入れておくことにより、使用時に冷却部材を取り出して該冷却部材に試料容器を収容し、その冷却部材を容器本体に収容することで使用可能となる。従って、本発明に係る凍結保冷器を用いることにより、尿や便といった生物学的試料を凍結させるために冷凍庫に入れる必要がなくなる。 According to the cryocooler according to the present invention, since the cooling member containing the cryogen having a melting point of −10 ° C. or lower is used, the temperature in the container body can be set to −10 ° C. or lower, and blood, urine, Biological samples such as stool can be frozen. In addition, since the container body has a heat insulating structure, it has a high cooling effect, and the biological sample can be kept in a frozen state for a long period of time. Furthermore, since the cooling member can be detachably accommodated in the container main body, by placing only the cooling member in the freezer in advance, the cooling member is taken out during use, and the sample container is accommodated in the cooling member. It can be used by being housed in the container body. Therefore, the use of the cryocooler according to the present invention eliminates the need to place biological samples such as urine and stool in a freezer.
また、上記理由と同様に、生物学的試料を凍結状態で長期間保冷することができるようにするために、保冷剤は、冷却部材に１００ｇ以上含まれていることが好ましい。 Further, for the same reason as described above, it is preferable that the cooling member is contained in an amount of 100 g or more so that the biological sample can be kept in a frozen state for a long period of time.
本発明に係る凍結保冷器は、収容部の開口を塞ぐ断熱性の内蓋部材をさらに備えていることが好ましい。 It is preferable that the cryocooler according to the present invention further includes a heat insulating inner lid member that closes the opening of the housing portion.
このようにすると、凍結保冷器の保冷効果をより向上することができる。 If it does in this way, the cold preservation effect of a freezing cooler can be improved more.
本発明に係る凍結保冷器では、上述の通り、生物学的試料を凍結させるために冷凍庫に入れる必要がないため、特に生物学的試料が便である場合に好適である。 As described above, the cryocooler according to the present invention does not need to be placed in a freezer in order to freeze the biological sample, and thus is particularly suitable when the biological sample is feces.
本発明に係る凍結保冷器によると、生物学的試料を凍結できると共に、冷凍状態で長期間保管することができる。 According to the cryocooler according to the present invention, a biological sample can be frozen and stored for a long time in a frozen state.
以下、本発明を実施するための形態を説明する。以下の好ましい実施形態の説明は、本質的に例示に過ぎず、本発明、その適用方法或いはその用途を制限することを意図するものではない。 Hereinafter, modes for carrying out the present invention will be described. The following description of the preferred embodiments is merely exemplary in nature and is not intended to limit the invention, its method of application, or its application.
まず、本発明の一実施形態に係る凍結保冷器について図面を参照しながら説明する。図１に示すように、本実施形態に係る凍結保冷器１は、断熱構造の容器本体２と、容器本体２に収容される冷却部材３と、容器本体２の開口を塞ぐ蓋部材４とを含む。冷却部材３には、生物学的試料が収容された試料容器１０を収容するために凹設された収容部３ａが設けられている。また、本実施形態において、収容部３ａの開口を塞いで収容部３ａ内の保冷効果を向上するための内蓋部材５が設けられている。 First, a cryocooler according to an embodiment of the present invention will be described with reference to the drawings. As shown in FIG. 1, the cryocooler 1 according to the present embodiment includes a container body 2 having a heat insulating structure, a cooling member 3 accommodated in the container body 2, and a lid member 4 that closes the opening of the container body 2. Including. The cooling member 3 is provided with an accommodating portion 3a that is recessed to accommodate the sample container 10 in which a biological sample is accommodated. Further, in the present embodiment, an inner lid member 5 is provided for closing the opening of the housing portion 3a to improve the cooling effect in the housing portion 3a.
容器本体２は、断熱性能に優れる構造であれば特に限定されないが、例えば真空断熱構造又は断熱材を間に挟んだ断熱二重構造であることが好ましい。断熱二重構造において間に挟まれる断熱材としては、以下のものに限定されないが、発泡スチロールや発泡ポリウレタン等の発泡プラスチック、又はガラス繊維やロックウォール等の無機繊維を用いることができる。また、断熱構造としては真空断熱構造や断熱二重構造以外に、容器全体が上記断熱材で形成されていてもよい。 Although it will not specifically limit if the container main body 2 is a structure excellent in heat insulation performance, For example, it is preferable that it is a heat insulation double structure which pinched | interposed the vacuum heat insulation structure or the heat insulating material. The heat insulating material sandwiched between the heat insulating double structures is not limited to the following, but may be foamed plastics such as foamed polystyrene and foamed polyurethane, or inorganic fibers such as glass fibers and lock walls. Moreover, as a heat insulation structure, the whole container may be formed with the said heat insulating material other than a vacuum heat insulation structure or a heat insulation double structure.
冷却部材３は、両端が塞がれた筒状の部材であり、その内部には保冷剤６が含まれている。保冷剤６は、凍結によりその体積が膨張することを鑑みて、冷却部材３内にその容積の８０〜９０％程度充填されている。また、冷却部材３の一端面（上面）には、上述の通り、内側に凹設された生物学的試料を含む試料容器１０を収容するための収容部３ａが形成されている。図１では、一つの収容部３ａが形成されているが、複数の収容部３ａが形成されていても構わない。冷却部材３は、比較的熱伝導率が高く、保冷剤の凍結融解に伴う体積変化に耐えうる材料からなり、例えばプラスチック樹脂からなることが好ましい。また、容器本体２内の冷却及び保冷効果を向上するために、冷却部材３の外形寸法は、冷却部材３が容器本体２内に収容可能な範囲で容器本体２の内径寸法に近似することが好ましい。冷却部材３は、容器本体２内に収容可能であると共に、容器本体２の外に取り出すことも可能なように構成されており、すなわち容器本体２内に着脱可能に収容される。 The cooling member 3 is a cylindrical member whose both ends are closed, and the inside of the cooling member 3 contains a cooling agent 6. In view of the fact that the volume of the cryogen 6 expands due to freezing, about 80 to 90% of the volume is filled in the cooling member 3. Further, as described above, an accommodation portion 3a for accommodating the sample container 10 containing the biological sample recessed inside is formed on one end surface (upper surface) of the cooling member 3. In FIG. 1, one accommodating portion 3 a is formed, but a plurality of accommodating portions 3 a may be formed. The cooling member 3 is made of a material having a relatively high thermal conductivity and capable of withstanding a volume change accompanying freezing and thawing of the cooling agent, and is preferably made of, for example, a plastic resin. Further, in order to improve the cooling and cooling effect in the container body 2, the outer dimension of the cooling member 3 can be approximated to the inner diameter dimension of the container body 2 as long as the cooling member 3 can be accommodated in the container body 2. preferable. The cooling member 3 can be accommodated in the container main body 2 and can be taken out of the container main body 2. That is, the cooling member 3 is detachably accommodated in the container main body 2.
保冷剤６は、生物学的試料を凍結させることができるように、生物学的試料を−１０℃程度にまで冷却できる材料であることが好ましく、このために、その融点が−１０℃以下である。また、同様に冷却効果が長期間維持できるようにするために、保冷剤６の量は１００ｇ以上であることが好ましい。 The cryogen 6 is preferably a material that can cool a biological sample to about −10 ° C. so that the biological sample can be frozen. For this reason, the melting point is −10 ° C. or lower. is there. Similarly, in order to maintain the cooling effect for a long period of time, the amount of the refrigerant 6 is preferably 100 g or more.
蓋部材４は、容器本体２の開口を塞ぎ、容器本体２内の保冷状態を維持するためのものである。蓋部材４は、凍結保冷器１の輸送の観点から軽量且つ破損し難い材料からなることが好ましく、例えばポリスチレン、ポリエチレン、ポリプロピレン等の樹脂製であることが好ましい。特に、蓋部材４と容器本体２との接続部分は、蓋部材４によって容器本体２の開口を塞ぐことができればどのような形態であっても構わず、図１のように単純に蓋部材４と容器本体２とを嵌合させてもよいし、蓋部材４と容器本体２のそれぞれの接続部分にねじ山を切って蓋部材４を回すことで開閉可能に構成してもよい。 The lid member 4 is for closing the opening of the container body 2 and maintaining the cold state in the container body 2. The lid member 4 is preferably made of a material that is lightweight and difficult to break from the viewpoint of transporting the cryocooler 1, and is preferably made of a resin such as polystyrene, polyethylene, or polypropylene. In particular, the connecting portion between the lid member 4 and the container main body 2 may be in any form as long as the lid member 4 can close the opening of the container main body 2, and the lid member 4 is simply as shown in FIG. And the container main body 2 may be fitted, or the lid member 4 and the container main body 2 may be configured to be openable and closable by turning the lid member 4 by cutting a screw thread at each connection portion.
内蓋部材５は、上述の通り冷却部材３の収容部３ａの開口を塞ぎ、収容部３ａ内の保冷状態を維持するためのものである。上記蓋部材４に加えて、内蓋部材５を用いることによって収容部３ａの保冷効果をより向上でき、収容部３ａに収容された生物学的試料を長期間保冷することが可能となる。このため、内蓋部材５は断熱性を有する材料からなることが好ましく、例えば発泡プラスチック製であることが好ましい。なお、内蓋部材５は、図１に示すように容器本体の開口に嵌合されてもよいし、上記蓋部材４と同様に、内蓋部材５の外周面及び容器本体２の上部内周面にねじ山を切って内蓋部材５を回すことで開閉可能に構成してもよい。なお、内蓋部材５は、蓋部材４と一体に構成されていてもよい。また、蓋部材４が、断熱性が高い材料で形成されている場合、内蓋部材５を省略しても構わない。 As described above, the inner lid member 5 closes the opening of the accommodating portion 3a of the cooling member 3 and maintains the cold state in the accommodating portion 3a. By using the inner lid member 5 in addition to the lid member 4, it is possible to further improve the cooling effect of the storage portion 3 a and to cool the biological sample stored in the storage portion 3 a for a long period of time. For this reason, the inner lid member 5 is preferably made of a heat-insulating material, for example, preferably made of foamed plastic. The inner lid member 5 may be fitted into the opening of the container main body as shown in FIG. 1, and, like the lid member 4, the outer peripheral surface of the inner lid member 5 and the upper inner periphery of the container main body 2. You may comprise so that opening and closing is possible by turning a screw thread in a surface and rotating the inner cover member 5. FIG. The inner lid member 5 may be configured integrally with the lid member 4. Moreover, when the lid member 4 is formed of a material having high heat insulation, the inner lid member 5 may be omitted.
次に、本実施形態に係る凍結保冷器１の使用方法について説明する。まず、凍結保冷器１の使用前に冷却部材３を予め冷凍庫に入れて、冷却部材３内の保冷剤６を冷凍しておく。そして、使用時において、その冷却部材３の収容部３ａ内に、凍結輸送するための生物学的試料を収容した収容容器１０を収容する。その後、該収容容器１０が収容された冷却部材３を容器本体２に収容し、内蓋部材５で収容部３ａの開口を塞いだ後、蓋部材４によって容器本体２の開口を塞ぐ。 Next, the usage method of the frozen cooler 1 which concerns on this embodiment is demonstrated. First, the cooling member 3 is put in a freezer in advance before using the cryocooler 1, and the cooling agent 6 in the cooling member 3 is frozen. In use, the storage container 10 storing a biological sample for freezing and transporting is stored in the storage portion 3a of the cooling member 3. Thereafter, the cooling member 3 in which the storage container 10 is stored is stored in the container body 2, the opening of the storage portion 3 a is closed with the inner lid member 5, and then the opening of the container body 2 is closed with the lid member 4.
以上のステップの結果、保冷剤６によって、容器本体２内における特に収容部３ａ内の温度が−１０℃以下にまで低減し、収容容器１０内の尿、血液又は便等の生物学的試料を凍結することができる。さらに、保冷剤６によって、その収容部３ａ内の温度を−１０℃以下で長期間維持されるため、上記生物学的試料を凍結状態で長期間維持できるため、例えば１０時間以上の長期の輸送に有利である。 As a result of the above steps, the temperature of the container body 2 in the container portion 2a is reduced to −10 ° C. or less by the cryogen 6 and biological samples such as urine, blood, or feces in the container 10 are removed. Can be frozen. Furthermore, since the temperature in the container 3a is maintained at −10 ° C. or lower for a long period of time by the cryogen 6, the biological sample can be maintained in a frozen state for a long period of time. Is advantageous.
また、本実施形態の凍結保冷器１によると、冷却部材３のみを予め冷凍庫に入れて保冷剤６を凍結させ、生物学的試料は容器本体２内に収容された冷却部材３の収容部３ａ内で凍結されるため、生物学的試料を冷凍庫に入れて凍結させる必要がない。従って、例えば在宅検診において便や尿を自宅で採取して凍結させた上で試験所に輸送する場合であっても、本実施形態の凍結保冷器１によれば、便や尿を家庭用の冷凍庫に入れる必要がなく、受診者に不快感を与えることがない。 Moreover, according to the cryocooler 1 of this embodiment, only the cooling member 3 is put in a freezer beforehand, the cryogen 6 is frozen, and the biological sample is accommodated in the container body 2 in the accommodating portion 3a of the cooling member 3. It is not necessary to put the biological sample in the freezer and freeze it. Therefore, for example, even when stool and urine are collected at home at home examination, frozen and transported to a laboratory, according to the cryocooler 1 of this embodiment, stool and urine are used for home use. There is no need to put it in the freezer, and there is no discomfort for the patient.
以下に、本発明に係る凍結保冷器を詳細に説明するための実施例を示す。本実施例では、本発明に係る凍結保冷器の保冷効果を評価するために、冷凍庫で凍結された冷却部材を含む凍結保冷器内の時間経過に従った温度上昇について測定した。以下にその方法と結果について説明する。 The Example for demonstrating in detail the freezing cooler which concerns on this invention below is shown. In this example, in order to evaluate the cooling effect of the freezing cooler according to the present invention, the temperature increase over time in the freezing cooler including the cooling member frozen in the freezer was measured. The method and result will be described below.
まず、試験対象となる実施例１、２及び比較例１の凍結保冷器の構成について説明する。 First, the structure of the cryocooler of Examples 1 and 2 and Comparative Example 1 to be tested will be described.
（実施例１）
実施例１において、容器本体としてステンレスからなる真空二重構造のイオン株式会社販売（品番：ＢＰ−１６ＦＪ４０ＢＫ）のステンレスフードジャーを用いた（直径８．３ｃｍ×１５．０ｃｍ、容量約４００ｍｌ）。なお、蓋部材は、当該容器本体に付属して販売される蓋部材を用いた。また、冷却部材としてアクリル樹脂からなる両端部が塞がれた円筒形部材を用いた。なお、冷却部材の寸法は、外径が６４ｍｍ、高さが９８ｍｍであり、収容部としての凹部の径は２３ｍｍであり、深さが８５ｍｍである。保冷剤は、融点が−１６℃であるＬＯＧＯＳ社製の氷点下パックＧＴ−１６℃を用い、冷却部材内に１９５ｇ充填した。内蓋部材としては上記容器本体の開口に対応する形状の発泡スチロールを用いた。
Example 1
In Example 1, the stainless steel hood jar (product number: BP-16FJ40BK) with a vacuum double structure made of stainless steel (diameter: 8.3 cm × 15.0 cm, capacity: about 400 ml) was used as the container body. The lid member used was a lid member sold with the container body. Moreover, the cylindrical member with which the both ends which consist of acrylic resins were block | closed was used as a cooling member. The dimensions of the cooling member are an outer diameter of 64 mm and a height of 98 mm, the diameter of the recess as the housing portion is 23 mm, and the depth is 85 mm. As the cooling agent, LOGOS sub-freezing pack GT-16 ° C having a melting point of -16 ° C was used, and 195 g was filled in the cooling member. As the inner lid member, a polystyrene foam having a shape corresponding to the opening of the container body was used.
（実施例２）
実施例２において、容器本体としてステンレスからなる真空二重構造の象印マホービン株式会社製のステンレスフードジャー（品番：ＳＷ−ＨＢ５５−ＮＬ）を用いた（直径９．０ｃｍ×１５．９ｃｍ、容量約５００ｍｌ）。なお、蓋部材は、当該容器本体に付属して販売される蓋部材を用いた。また、冷却部材としてアクリル樹脂からなる両端部が塞がれた円筒形部材を用いた。なお、冷却部材の寸法は、外径が６４ｍｍ、高さが１１２ｍｍであり、収容部としての凹部の径は２３ｍｍであり、深さが９４ｍｍである。保冷剤は、融点が−１６℃であるＬＯＧＯＳ社製の氷点下パックＧＴ−１６℃を用い、冷却部材内に２３０ｇ充填した。内蓋部材としては上記容器本体の開口に対応する形状の発泡スチロールを用いた。
(Example 2)
In Example 2, a stainless steel hood jar (product number: SW-HB55-NL) manufactured by ZOJIRUSHI CORP. Made of stainless steel was used as the container body (diameter: 9.0 cm × 15.9 cm, capacity: about 500 ml). ). The lid member used was a lid member sold with the container body. Moreover, the cylindrical member with which the both ends which consist of acrylic resins were block | closed was used as a cooling member. The dimensions of the cooling member are an outer diameter of 64 mm and a height of 112 mm, a diameter of the recess as the housing portion is 23 mm, and a depth of 94 mm. As the cooling agent, a sub-freezing pack GT-16 ° C. made by LOGOS having a melting point of −16 ° C. was used, and 230 g was filled in the cooling member. As the inner lid member, a polystyrene foam having a shape corresponding to the opening of the container body was used.
（比較例）
比較例において、容器本体として発泡スチロールからなる円筒状容器を用いた。なお、容器本体の直径は１０ｃｍ、高さは１４ｃｍとし、その外壁の厚さを１．５ｃｍとした。蓋部材は、発泡スチロールからなり、上記容器本体の開口を塞ぐようにまた、冷却部材としてアクリル樹脂からなる両端部が塞がれた円筒形部材を用いた。なお、冷却部材の寸法は、上記実施例２と同一である。保冷剤は、融点が−１６℃であるＬＯＧＯＳ社製の氷点下パックＧＴ−１６℃を用い、冷却部材内に２３０ｇ充填した。比較例では、上述の通り、蓋部材を断熱性能が高い発泡スチロールで構成しているため、発泡スチロール製の内蓋部材を設けなかった。
(Comparative example)
In the comparative example, a cylindrical container made of polystyrene foam was used as the container body. The container body had a diameter of 10 cm, a height of 14 cm, and an outer wall thickness of 1.5 cm. The lid member was made of styrene foam, and a cylindrical member with both end portions made of acrylic resin being closed was used as a cooling member so as to close the opening of the container body. The dimensions of the cooling member are the same as those in the second embodiment. As the cooling agent, a sub-freezing pack GT-16 ° C. made by LOGOS having a melting point of −16 ° C. was used, and 230 g was filled in the cooling member. In the comparative example, as described above, since the lid member is made of foamed polystyrene having high heat insulation performance, the foamed polystyrene inner lid member was not provided.
（温度測定試験）
上記実施例１、２及び比較例の凍結保冷器について、保冷能力を評価する試験を以下の通りに行った。まず、冷却部材を庫内が−２０℃である冷凍庫に３日間入れて冷却部材内の保冷剤を凍結させた。その後、冷却部材を冷凍庫から出し、冷却部材の収容部に小型温度ロガーを入れ、冷却部材を容器本体内に収容して蓋部材で密閉した。密閉後の容器本体を庫内が３０℃のインキュベーターに入れ所定時間毎に温度ロガーを取り出して、容器本体内の温度をチェックした。
(Temperature measurement test)
About the frozen cooler of the said Examples 1 and 2 and a comparative example, the test which evaluates cold storage capability was done as follows. First, the cooling member was placed in a freezer having a temperature of −20 ° C. for 3 days to freeze the cooling agent in the cooling member. Thereafter, the cooling member was taken out of the freezer, a small temperature logger was put in the housing part of the cooling member, the cooling member was housed in the container body, and sealed with a lid member. The container body after sealing was placed in an incubator whose inside was 30 ° C., and the temperature logger was taken out every predetermined time to check the temperature inside the container body.
上記試験の結果、実施例１の凍結保冷器では、最初は容器内が−２０℃であったが、徐々に温度が上昇し、インキュベーターに入れられてから２１時間５５分間は−１０℃以下を維持し、その後−１０℃を超える温度となった。また、実施例２の凍結保冷器においても最初は容器内が−２０℃であったが、徐々に温度が上昇し、インキュベーターに入れられてから３０時間３０分間は−１０℃以下を維持し、その後−１０℃を超える温度となった。一方、比較例の凍結保冷器においては試験開始から５時間４５分で−１０℃を超える温度となった。 As a result of the above test, in the cryocooler of Example 1, the inside of the container was initially -20 ° C, but the temperature gradually increased and the temperature was kept below -10 ° C for 21 hours and 55 minutes after being placed in the incubator. Maintained, and then the temperature exceeded -10 ° C. Also, in the cryocooler of Example 2, the inside of the container was initially -20 ° C, but the temperature gradually increased and maintained at -10 ° C or lower for 30 hours and 30 minutes after being placed in the incubator, Thereafter, the temperature exceeded -10 ° C. On the other hand, in the cryocooler of the comparative example, the temperature exceeded −10 ° C. in 5 hours 45 minutes from the start of the test.
以上の結果から、本発明に係る凍結保冷器によると、３０℃という比較的高い外気温環境下においても、容器内の温度を長期間−１０℃以下に維持できることが分かる。また、１９５ｇの保冷剤を用いることで−１０℃以下に２０時間以上維持できるため、約１００ｇの保冷剤を用いれば−１０℃以下に１０時間以上の長期間維持できると考えられる。 From the above results, it can be seen that according to the cryocooler according to the present invention, the temperature in the container can be maintained at −10 ° C. or lower for a long time even in a relatively high outside air temperature environment of 30 ° C. Moreover, since it can maintain at -10 degrees C or less for 20 hours or more by using 195 g of a cold insulating agent, if it uses about 100 g of cold insulating agents, it is thought that it can maintain for 10 hours or more at -10 degrees C or less.
以上の通り、本発明に係る凍結保冷器によると、その保冷器自体で例えば便や尿や血液といった生物学的試料を凍結させることが可能であり、さらに、凍結状態で長期間維持することができる。 As described above, according to the cryocooler according to the present invention, it is possible to freeze biological samples such as stool, urine and blood with the cooler itself, and it can be maintained in a frozen state for a long period of time. it can.
１ 凍結保冷器
２ 容器本体
３ 冷却部材
３ａ 収容部
４ 蓋部材
５ 内蓋部材
６ 保冷剤
１０ 収容容器
DESCRIPTION OF SYMBOLS 1 Freezing cooler 2 Container main body 3 Cooling member 3a Accommodating part 4 Lid member 5 Inner lid member 6 Coolant 10 Receiving container
Claims (4)
断熱構造の容器本体と、
前記容器本体に着脱可能に収容され、内部に保冷剤を含む冷却部材と、
前記容器本体の開口を塞ぐ蓋部材とを備え、
前記冷却部材は、前記生物学的試料を含む試料容器を収容するための収容部を有し、
前記保冷剤は、融点が−１０℃以下であることを特徴とする凍結保冷器。 A cryocooler for freezing a biological sample and keeping it cold,
An insulated container body,
A cooling member that is detachably housed in the container body and contains a cryogen inside;
A lid member for closing the opening of the container body,
The cooling member has an accommodating portion for accommodating a sample container containing the biological sample,
The cryoprotector has a melting point of −10 ° C. or lower.
The cryopreservation device according to any one of claims 1 to 3, wherein the biological sample is feces.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110305944A (en) * | 2019-06-26 | 2019-10-08 | 王中刚 | A kind of cool-down method for hereditary tumor genetic test |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110305944A (en) * | 2019-06-26 | 2019-10-08 | 王中刚 | A kind of cool-down method for hereditary tumor genetic test |
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