JP2018000048A - 多能性幹細胞の無染色評価支援方法、プログラム、演算装置 - Google Patents
多能性幹細胞の無染色評価支援方法、プログラム、演算装置 Download PDFInfo
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Abstract
Description
2、31、51、71 照明光学系
3、37、55、77 結像光学系
4、32、52、72 光源
5、33、73 コレクタレンズ
6、34、53、76 コンデンサレンズ
7、11、36、39、53、57、75、79 ミラー
8、13 偏光板
9、12 DICプリズム
10、38、56、78 対物レンズ
14、41、58、81 結像レンズ
15、42、59、82 撮像素子
16 λ/4板
20、43、60、83 演算装置
21 通信部
22 記憶部
23 演算部
35 リングスリット
80 遮光手段
80a 開口部
84 瞳
85、A、B、C、D 領域
Claims (9)
- 位相分布を像強度分布に変換する顕微鏡によって撮像された生体標本の画像信号から前記生体標本の第1の位相分布を算出する工程と、
前記第1の位相分布から特定の位相量以上の位相量を有する領域を抽出する工程と、
前記特定の位相量以上の位相量を有する領域を用いて、前記生体標本の状態をユーザが評価するための指標となる評価情報を作成し、前記評価情報を提示する工程と、を含み、
前記特定の位相量は、体細胞のミトコンドリアの位相量である
ことを特徴とする多能性幹細胞の無染色評価支援方法。 - 請求項1に記載の無染色評価支援方法であって、
前記第1の位相分布の画像中の前記特定の位相量以上の位相量を有する領域を他の領域と区別することで、前記評価情報を作成する
ことを特徴とする多能性幹細胞の無染色評価支援方法。 - 請求項1に記載の無染色評価支援方法であって、
前記生体標本の特定の領域において、前記特定の位相量以上の位相量を有する領域が占有する面積比率を算出することで、前記評価情報を作成する
ことを特徴とする多能性幹細胞の無染色評価支援方法。 - 請求項1乃至請求項3のいずれか1項に記載の無染色評価支援方法であって、
位相分布を像強度分布に変換する前記顕微鏡として微分干渉顕微鏡を用いる
ことを特徴とする多能性幹細胞の無染色評価支援方法。 - 請求項1乃至請求項3のいずれか1項に記載の無染色評価支援方法であって、
位相分布を像強度分布に変換する前記顕微鏡として位相差顕微鏡を用いる
ことを特徴とする多能性幹細胞の無染色評価支援方法。 - 請求項1乃至請求項3のいずれか1項に記載の無染色評価支援方法であって、
位相分布を像強度分布に変換する前記顕微鏡として偏斜照明顕微鏡を用いる
ことを特徴とする多能性幹細胞の無染色評価支援方法。 - 請求項1乃至請求項3のいずれか1項に記載の無染色評価支援方法であって、
位相分布を像強度分布に変換する前記顕微鏡として偏心開口顕微鏡を用いる
ことを特徴とする多能性幹細胞の無染色評価支援方法。 - コンピュータを、
位相分布を像強度分布に変換する顕微鏡によって撮像された生体標本の画像信号を受信する手段と、
前記画像信号から前記生体標本の第1の位相分布を算出する手段と、
前記第1の位相分布から特定の位相量以上の位相量を有する領域を抽出する手段と、
前記特定の位相量以上の位相量を有する領域を用いて、前記生体標本の状態をユーザが評価するための指標となる評価情報を作成し、前記評価情報を出力する手段として機能させ、
前記特定の位相量は、体細胞のミトコンドリアの位相量である
ことを特徴とするプログラム。 - 位相分布を像強度分布に変換する顕微鏡によって撮像された生体標本の画像信号を受信し、表示媒体へデータを出力する通信部と、
前記画像信号から前記生体標本の第1の位相分布を算出する演算部と、を備え、
前記演算部は、前記第1の位相分布から特定の位相量以上の位相量を有する領域を抽出し、前記特定の位相量以上の位相量を有する領域を用いて、前記生体標本の状態をユーザが評価するための指標となる評価情報を作成し、
前記通信部は、前記評価情報を前記表示媒体へ出力し、
前記特定の位相量は、体細胞のミトコンドリアの位相量である
ことを特徴とする演算装置。
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WO2020145353A1 (ja) * | 2019-01-10 | 2020-07-16 | グリー株式会社 | コンピュータプログラム、サーバ装置、端末装置及び音声信号処理方法 |
CN112236705A (zh) * | 2018-05-30 | 2021-01-15 | 西门子医疗保健诊断公司 | 借助于光场相机对医学样本进行三维分析的分析仪 |
WO2021085034A1 (ja) | 2019-10-28 | 2021-05-06 | 富士フイルム株式会社 | 多能性幹細胞の選別方法、分化誘導結果の予測方法及び細胞製品の製造方法 |
WO2021085033A1 (ja) | 2019-10-28 | 2021-05-06 | 富士フイルム株式会社 | 多能性幹細胞の選別方法、分化誘導結果の予測方法及び細胞製品の製造方法 |
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US10931899B1 (en) | 2019-07-22 | 2021-02-23 | Bae Systems Information And Electronic Systems Integration Inc. | Systems and methods for simulation of shearographic imaging |
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Cited By (5)
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CN112236705A (zh) * | 2018-05-30 | 2021-01-15 | 西门子医疗保健诊断公司 | 借助于光场相机对医学样本进行三维分析的分析仪 |
JP2021525866A (ja) * | 2018-05-30 | 2021-09-27 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド | ライトフィールドカメラを用いて医学的試料を三次元的に分析するための分析器 |
WO2020145353A1 (ja) * | 2019-01-10 | 2020-07-16 | グリー株式会社 | コンピュータプログラム、サーバ装置、端末装置及び音声信号処理方法 |
WO2021085034A1 (ja) | 2019-10-28 | 2021-05-06 | 富士フイルム株式会社 | 多能性幹細胞の選別方法、分化誘導結果の予測方法及び細胞製品の製造方法 |
WO2021085033A1 (ja) | 2019-10-28 | 2021-05-06 | 富士フイルム株式会社 | 多能性幹細胞の選別方法、分化誘導結果の予測方法及び細胞製品の製造方法 |
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