JP2013540804A - 薬剤送達組成物 - Google Patents
薬剤送達組成物 Download PDFInfo
- Publication number
- JP2013540804A JP2013540804A JP2013535476A JP2013535476A JP2013540804A JP 2013540804 A JP2013540804 A JP 2013540804A JP 2013535476 A JP2013535476 A JP 2013535476A JP 2013535476 A JP2013535476 A JP 2013535476A JP 2013540804 A JP2013540804 A JP 2013540804A
- Authority
- JP
- Japan
- Prior art keywords
- cellulose
- cnf
- nanofibers
- hydrogel
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 53
- 238000012377 drug delivery Methods 0.000 title claims abstract description 24
- 229920002678 cellulose Polymers 0.000 claims abstract description 145
- 239000001913 cellulose Substances 0.000 claims abstract description 145
- 239000002121 nanofiber Substances 0.000 claims abstract description 115
- 239000000017 hydrogel Substances 0.000 claims abstract description 104
- 239000000463 material Substances 0.000 claims abstract description 44
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 12
- 239000003814 drug Substances 0.000 claims description 36
- 229940079593 drug Drugs 0.000 claims description 36
- 239000002994 raw material Substances 0.000 claims description 17
- 229920001340 Microbial cellulose Polymers 0.000 claims description 9
- 238000007918 intramuscular administration Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 230000000699 topical effect Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000007920 subcutaneous administration Methods 0.000 claims description 7
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000000969 carrier Substances 0.000 claims 1
- 239000000499 gel Substances 0.000 description 24
- 210000004027 cell Anatomy 0.000 description 23
- 239000006185 dispersion Substances 0.000 description 21
- 239000000047 product Substances 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 11
- 229940088679 drug related substance Drugs 0.000 description 11
- 239000000725 suspension Substances 0.000 description 11
- 238000009792 diffusion process Methods 0.000 description 10
- 229920002749 Bacterial cellulose Polymers 0.000 description 9
- 239000008186 active pharmaceutical agent Substances 0.000 description 9
- 239000005016 bacterial cellulose Substances 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 9
- 238000002347 injection Methods 0.000 description 9
- 239000007924 injection Substances 0.000 description 9
- 238000011084 recovery Methods 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 8
- 239000000835 fiber Substances 0.000 description 8
- 239000011159 matrix material Substances 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 7
- 239000002245 particle Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 108010059892 Cellulase Proteins 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000010008 shearing Methods 0.000 description 5
- 229920002307 Dextran Polymers 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000000604 cryogenic transmission electron microscopy Methods 0.000 description 4
- 210000004748 cultured cell Anatomy 0.000 description 4
- 230000007071 enzymatic hydrolysis Effects 0.000 description 4
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000012384 transportation and delivery Methods 0.000 description 4
- 238000012604 3D cell culture Methods 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 238000007385 chemical modification Methods 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007515 enzymatic degradation Effects 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 108010084185 Cellulases Proteins 0.000 description 2
- 102000005575 Cellulases Human genes 0.000 description 2
- 229920003043 Cellulose fiber Polymers 0.000 description 2
- 101710112457 Exoglucanase Proteins 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241001602876 Nata Species 0.000 description 2
- 229920002201 Oxidized cellulose Polymers 0.000 description 2
- 108010067520 RADA16-I Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- BLFLLBZGZJTVJG-UHFFFAOYSA-N benzocaine Chemical compound CCOC(=O)C1=CC=C(N)C=C1 BLFLLBZGZJTVJG-UHFFFAOYSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 206010061592 cardiac fibrillation Diseases 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000006196 drop Substances 0.000 description 2
- 239000003974 emollient agent Substances 0.000 description 2
- 230000002600 fibrillogenic effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011121 hardwood Substances 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 108010082117 matrigel Proteins 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000008104 plant cellulose Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 2
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 2
- 229960003415 propylparaben Drugs 0.000 description 2
- 238000000518 rheometry Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000011122 softwood Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 238000011421 subcutaneous treatment Methods 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- 230000000930 thermomechanical effect Effects 0.000 description 2
- 238000003828 vacuum filtration Methods 0.000 description 2
- 229920003169 water-soluble polymer Polymers 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000589212 Acetobacter pasteurianus Species 0.000 description 1
- 244000235858 Acetobacter xylinum Species 0.000 description 1
- 244000198134 Agave sisalana Species 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 241000609240 Ambelania acida Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- 241000219495 Betulaceae Species 0.000 description 1
- 240000008564 Boehmeria nivea Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 240000000491 Corchorus aestuans Species 0.000 description 1
- 235000011777 Corchorus aestuans Nutrition 0.000 description 1
- 235000010862 Corchorus capsularis Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 235000014466 Douglas bleu Nutrition 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 244000004281 Eucalyptus maculata Species 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 240000000797 Hibiscus cannabinus Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241000218652 Larix Species 0.000 description 1
- 235000005590 Larix decidua Nutrition 0.000 description 1
- 240000007472 Leucaena leucocephala Species 0.000 description 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 229920001410 Microfiber Polymers 0.000 description 1
- 229920001046 Nanocellulose Polymers 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000183024 Populus tremula Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 240000001416 Pseudotsuga menziesii Species 0.000 description 1
- 235000005386 Pseudotsuga menziesii var menziesii Nutrition 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000003021 Tsuga heterophylla Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000037374 absorbed through the skin Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229940035676 analgesics Drugs 0.000 description 1
- 229940035674 anesthetics Drugs 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000000730 antalgic agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 229960005274 benzocaine Drugs 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 125000001547 cellobiose group Chemical group 0.000 description 1
- 150000001773 cellobioses Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000013267 controlled drug release Methods 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 238000006266 etherification reaction Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 229940012356 eye drops Drugs 0.000 description 1
- 108060002894 fibrillar collagen Proteins 0.000 description 1
- 102000013373 fibrillar collagen Human genes 0.000 description 1
- 238000004362 fungal culture Methods 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 239000003193 general anesthetic agent Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 239000000416 hydrocolloid Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000007972 injectable composition Substances 0.000 description 1
- 150000007529 inorganic bases Chemical class 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002690 local anesthesia Methods 0.000 description 1
- 230000005541 medical transmission Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000013081 microcrystal Substances 0.000 description 1
- 210000001724 microfibril Anatomy 0.000 description 1
- 108700005457 microfibrillar Proteins 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 239000002159 nanocrystal Substances 0.000 description 1
- 239000002073 nanorod Substances 0.000 description 1
- 239000002070 nanowire Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 229940107304 oxidized cellulose Drugs 0.000 description 1
- 239000003961 penetration enhancing agent Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002207 retinal effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000807 solvent casting Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000013269 sustained drug release Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000012385 systemic delivery Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L1/00—Compositions of cellulose, modified cellulose or cellulose derivatives
- C08L1/02—Cellulose; Modified cellulose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L97/00—Compositions of lignin-containing materials
- C08L97/02—Lignocellulosic material, e.g. wood, straw or bagasse
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2301/00—Characterised by the use of cellulose, modified cellulose or cellulose derivatives
- C08J2301/02—Cellulose; Modified cellulose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/78—Cellulose
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Dispersion Chemistry (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Pharmacology & Pharmacy (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Nanotechnology (AREA)
- Ophthalmology & Optometry (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
Abstract
【選択図】なし
Description
ヘルスケアは、依然として科学研究の重要なフロンティアである。費用対効果の高い、より安全な薬剤を発見し、開発する必要性がこれまでに増加し続けている。
本発明は、セルロースナノファイバー及び/又はその誘導体を含み、該セルロースナノファイバー又はその誘導体がヒドロゲル又は膜の形態である、薬剤送達組成物に関する。セルロースナノファイバー又はその誘導体は、植物材料又は微生物性セルロースを含む原料、又は細菌性セルロース(BC)として一般に知られる細菌の発酵過程に由来する原料等の非動物ベースの材料から得ることができる。
植物由来セルロース及び微生物性セルロースは、局所的薬剤送達組成物において使用し得る。注入可能な生産物においては、植物由来セルロース(特に好ましくは天然の又は非イオン性のグレード)の使用が好ましい。
CNFヒドロゲルの調製
不透明な天然CNFヒドロゲル(1.7重量%)を、湿セルロースパルプファイバーの高圧ホモジナイゼーションにより得た。従って、該過程からの直接の生産物は希釈セルロースナノファイバーヒドロゲルである。透明なCNFヒドロゲル(0.9重量%)は、化学的に修飾された(TEMPO−酸化した)セルロースパルプの同様のホモジナイゼーション過程により得た。該サンプルはオートクレーブで滅菌した。細胞の研究については、該CNFヒドロゲルを適切な濃度に希釈し、機械的な混合又はソニケーションでホモジナイズした。天然CNF及び透明CNFのクライオ−TEMの写真を図1に示す。天然のセルロースナノファイバーヒドロゲルは、個々のセルロースナノフィブリル及びファイバーの束の混合物で構成される(図1A)。最も小さいファイバーの直径はおよそ7nmであるが、セルロース材料の大部分は束になった構造内で50〜100nmを形成している。正確な長さのスケールは、材料の絡み合い束ねられている性質に起因して、写真から推定することはできないが、個々のナノファイバーが数マイクロメーター長であることは明白であると思われる。光学的に透明なCNFヒドロゲルのクライオ−TEMの写真は、均一に分布した個々のセルロースナノファイバーのネットワークを示す。これらのナノファイバーの直径はおよそ7nmであり、長さは1マイクロメーターを超える。該ナノファイバーは、100〜200nm長の直線部分を有し、ファイバーのアクセル(axel)に沿って急なねじれが続く。これらの直線部分は高結晶質のセルロース領域からなる−曲がっている部分は非結晶部により形成される。
CNF膜は、水性0.2重量%の天然CNFの分散液の真空濾過により調製した。濾過後、湿った膜を55℃のオーブンで48時間、重りの下で乾燥させた。乾燥した薄い膜は、坪量70〜80g/m2で、滑らかで不透明であった。
CNFヒドロゲルの酵素分解を、Celluclast 1.5 LFG, CCN0367(Novozymes, pHopt 5)、Prot.90mg/mlを用いて0.5%ヒドロゲルを含む砂利を加水分解することにより示した。天然CNFの分解をpH5、50℃で4日間、及び透明CNFの分解をpH7、21℃で1時間行った。酵素量は1グラムのCNFに対し5mgの酵素であった。
CNFヒドロゲルを介したデキストランの拡散
材料の拡散特性についての詳細な知見は重要である。細胞培養材料は、栄養分及び酸素を培養細胞へ拡散させるのを可能にするため、及び細胞からの代謝物の効率的な拡散を可能にするために十分に多孔性であるべきである。CNFヒドロゲルの拡散特性は、以下の方法で様々な分子量のデキストランを用いて示した:
ゲル強度
培地の重要な機能は沈降を防ぐことである。CNFは、非常に低い濃度(0.5%)でゲルネットワークを形成するその性能により、この要求を満たす。CNFのゲル様構造は、動的振動流動学的測定によりその粘弾性特性を決定することで示された。振動数掃引の結果は、典型的なゲル様の挙動を示す。貯蔵率(G’)は、損失率(G’)よりも桁違いに高く、振動数にはほぼ無関係であり、これは弾性(固体様)特性が、粘性(液体様)の特徴よりも、より明白であることを意味する(図3)。また、ゲルは通常、G’及びG’’が両方とも振動数とは相対的に無関係である。CNFゲルの粘弾性特性は、0.1%の歪み(strain)でのレオメーター(AR-G2, TA Instruments)による発振振動数掃引測定で決定した。
CNFヒドロゲルの流動特性
CNFヒドロゲルの流動学的な流動特性は、いくつかの有益な特徴をもたらす。該ヒドロゲルは、低せん断(又は静止)では、細胞の最適な懸濁能力のための、高い粘度を有するが、より高いせん断率ではせん断で粘度が減少する挙動も示し、容易な分配及び注入を可能にする。これらの種の流動学的特性を提供するCNFの性能は、CNF分散液の粘度を回転式レオメーター(AR-G2, TA Instruments, UK)で広範なせん断応力(率)範囲に渡って測定した試験シリーズにおいて示された。
せん断停止後の構造回復
CNFヒドロゲルのさらなる重要な流動学的特性は、せん断(例えば、注入又は混合)が停止した後に、高レベルの粘度が保持されることである。CNF分散液の構造回復は、初めに材料をレオメーター(StressTech, Reologica Instruments Ab)内で、高せん断率でせん断し、せん断を停止した後、ゲル強度(G’)の回復を振動時間掃引測定で観測する試験シリーズにより示された。せん断サイクルは、同心円筒形状内で40Paの一定応力で61秒間行った。この試験の間のせん断率及び粘度の漸進的変化を図7に示す。当該材料を、相対的に高いせん断率(1000s−1)で、少なくとも40sの時間せん断し、その間に、該材料の粘度は40mPa sを下回るまで下降した。
安定性
CNFの非常に希薄な分散液でさえ、低せん断率で非常に高い粘度を有する。注入等のせん断を停止すると、ヒドロゲル構造も回復する。静止状態において、CNFは高い弾性率及び非常に高い降伏応力を有するヒドロゲルネットワークを形成する。これらの特性に起因して、CNFは、非常に低濃度においてでさえ、固体粒子の非常に高い懸濁力を有する。
酵素加水分解
特定の酵素(セルラーゼ)はセルロース中のβ−(1−4)−結合を加水分解することができることが一般に知られている。例えば、鎖の末端から離れたランダムな位置においてセルロース鎖を標的とするエンド−1,4−β−グルカナーゼ(EG);セロビオースダイマーを産生しながら鎖の両端から分子を切り離すことによりセルロースを分解するエキソグルカナーゼ又はエキソセロビオヒドロラーゼ(CBH);及びセロビオース単位(EG及びCBHの攻撃間に産生される)をグルコースへ加水分解するβ−グルコシダーゼ(BGL)。それぞれ、セルロースナノファイバーは、セルラーゼを活用して酵素的にグルコースへ加水分解され得る(Ahola, S., Turon, X., Osterberg, M., Laine, J., Rojas, O.J., Langmuir, 2008, 24, 11592-11599)。
眼用組成物
天然セルロースナノファイバーに基づく0.5重量%ヒドロゲルを、精製水を使用して作製する。眼での使用に適した均一な分散液を得るために、0.1重量%のヒアルロン酸、0.1重量%のEDTA、0.2重量%のプロピルパラベン、0.2重量%のTween 80、及び0.8重量%のグリセロールをヒドロゲル中に取り込む。あるいは、該成分を精製水中に取り込み、次いで、セルロースナノファイバーと混合し得る。同様の方法で、例えば網膜の圧力を制御するため、又はその他の適応症のための薬剤を含む組成物を得ることができる。
局所麻酔用組成物
天然セルロースナノファイバーに基づく1.2重量%ヒドロゲルを、精製水を使用して作製する。0.2重量%のプロピルパラベン及び15重量%のベンゾカインを、薬剤の溶解を補助するためのエタノールとともに、ヒドロゲル中に取り込む。あるいは、原薬はアルコールなしでヒドロゲル中に粒子形態で分散することができ、それにより、薬剤の制御された放出が達成される。
Claims (12)
- ヒドロゲル又は膜の形態であるセルロースナノファイバー及び/又はその誘導体、並びに薬剤又は薬物を含むことを特徴とする、薬剤送達組成物。
- セルロースナノファイバー及び/若しくはその誘導体の直径、又は該セルロースナノファイバーにおけるナノファイバーの束の直径が、1μm未満、好ましくは200nm未満、より好ましくは100nm未満であることを特徴とする、請求項1に記載の組成物。
- セルロースナノファイバー誘導体が、セルロースナノファイバー又はナノファイバーの束の化学的に又は物理的に修飾された誘導体を含むことを特徴とする、請求項1又は2に記載の組成物。
- セルロースナノファイバー及び/又はその誘導体が、植物材料又は微生物性セルロースを含む原料、又は細菌の発酵過程に由来する原料から得られることを特徴とする、請求項1〜3のいずれか1項に記載の組成物。
- 組成物が、局所的、皮下での、筋肉内での、又は眼内での組成物であることを特徴とする、請求項1〜4のいずれか1項に記載の組成物。
- −セルロースナノファイバー及び/又はその誘導体を提供する工程、
−前記セルロースナノファイバーを水とともに混合する工程、
−該混合物を薬物又は薬剤と組み合わせて組成物を得る工程、
を含むことを特徴とする、請求項1〜4のいずれか1項に記載の薬剤送達組成物を製造する方法。 - 該方法が、得られた組成物を薬剤送達のための適切な環境へ移動させること又は配置することをさらに含むことを特徴とする、請求項6に記載の方法。
- セルロースナノファイバー及び/又はその誘導体、或いは該セルロースナノファイバー及び/又はその誘導体を含むヒドロゲル又は膜の、薬剤送達組成物における担体としての使用。
- セルロースナノファイバー及び/若しくはその誘導体の直径、又は該セルロースナノファイバーにおけるナノファイバーの束の直径が、1μm未満、好ましくは200nm未満、より好ましくは100nm未満であることを特徴とする、請求項8に記載の使用。
- セルロースナノファイバー誘導体が、セルロースナノファイバー又はナノファイバーの束の化学的又は物理的に修飾された誘導体を含むことを特徴とする請求項8又は9に記載の使用。
- セルロースナノファイバー及び/又はその誘導体が、植物材料又は微生物性セルロースを含む原料、又は細菌の発酵過程に由来する原料から得られることを特徴とする、請求項8〜10のいずれか1項に記載の使用。
- 組成物が、局所的、皮下での、筋肉内での、又は眼内での組成物であることを特徴とする、請求項8〜11のいずれか1項に記載の使用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI20106121A FI123988B (fi) | 2010-10-27 | 2010-10-27 | Soluviljelymateriaali |
FI20106121 | 2010-10-27 | ||
PCT/FI2011/050941 WO2012056111A2 (en) | 2010-10-27 | 2011-10-26 | Drug delivery compositions |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013540804A true JP2013540804A (ja) | 2013-11-07 |
JP6321961B2 JP6321961B2 (ja) | 2018-05-09 |
Family
ID=43064279
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2013535476A Active JP6321961B2 (ja) | 2010-10-27 | 2011-10-26 | 薬剤送達組成物 |
JP2013535475A Pending JP2013541956A (ja) | 2010-10-27 | 2011-10-26 | 植物由来の細胞培養材料 |
JP2017241022A Active JP6448755B2 (ja) | 2010-10-27 | 2017-12-15 | 植物由来の細胞培養材料 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2013535475A Pending JP2013541956A (ja) | 2010-10-27 | 2011-10-26 | 植物由来の細胞培養材料 |
JP2017241022A Active JP6448755B2 (ja) | 2010-10-27 | 2017-12-15 | 植物由来の細胞培養材料 |
Country Status (16)
Country | Link |
---|---|
US (3) | US20140010790A1 (ja) |
EP (4) | EP2975115B1 (ja) |
JP (3) | JP6321961B2 (ja) |
CN (2) | CN107557330B (ja) |
AU (1) | AU2011322363B2 (ja) |
BR (1) | BR112013010377B1 (ja) |
CA (1) | CA2815276C (ja) |
DK (3) | DK2633032T3 (ja) |
ES (3) | ES2759257T3 (ja) |
FI (1) | FI123988B (ja) |
HK (1) | HK1188466A1 (ja) |
IL (1) | IL225923B (ja) |
PL (2) | PL2975115T3 (ja) |
RU (1) | RU2597978C2 (ja) |
SG (1) | SG189966A1 (ja) |
WO (3) | WO2012056109A2 (ja) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2017532384A (ja) * | 2014-10-24 | 2017-11-02 | サントル ナスィオナル ド ラ ルシェルシュ スィアンティフィク(セ.エン.エル.エス.) | 時間制御型グルコース放出ヒドロゲル及びそれらの用途 |
JP2021507913A (ja) * | 2017-12-21 | 2021-02-25 | エイチ アンド エー ファーマケム カンパニー,リミテッドH&A Pharmachem Co., Ltd | 金属有機構造体及びナノセルロースを用いた経皮送達用複合体 |
JP2021046393A (ja) * | 2019-09-13 | 2021-03-25 | ユー ピー エム キュンメネ コーポレーション | 注射可能な医薬製剤 |
JP2022530057A (ja) * | 2019-04-22 | 2022-06-27 | イーエルシー マネージメント エルエルシー | セルロースナノファイバーを含有する局所送達系 |
JP2022538278A (ja) * | 2019-06-24 | 2022-09-01 | ユー ピー エム キュンメネ コーポレーション | ナノフィブリルセルロースヒドロゲル中に真核細胞を含む移植可能な細胞組成物、その調製方法、及びナノフィブリルセルロースの使用 |
Families Citing this family (70)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI123988B (fi) | 2010-10-27 | 2014-01-31 | Upm Kymmene Corp | Soluviljelymateriaali |
FI130619B (en) | 2011-11-15 | 2023-12-15 | Upm Kymmene Corp | Matrix for controlled release of bioactive substances |
FI123694B (en) * | 2011-12-22 | 2013-09-30 | Upm Kymmene Corp | Matrix and composition for microbial culture of gram-positive bacteria |
FI123715B (en) * | 2011-12-22 | 2013-09-30 | Upm Kymmene Corp | Composition for embedded microbial culture |
FI126055B (en) * | 2012-05-14 | 2016-06-15 | Upm Kymmene Corp | A method of making a film from fibril pulp and a fibril pulp film |
FI125965B (en) * | 2012-09-25 | 2016-04-29 | Upm Kymmene Corp | Three-dimensional cell culture |
JP5846449B2 (ja) * | 2012-11-12 | 2016-01-20 | 北越紀州製紙株式会社 | 電池用セパレータの製造方法及び電池用セパレータ |
CN102978256B (zh) * | 2012-12-26 | 2013-11-20 | 东华大学 | 一种连续生产细菌纤维素的方法 |
FI126109B (en) | 2013-02-22 | 2016-06-30 | Upm Kymmene Corp | Nanofibril polysaccharide for use in the control and prevention of constriction and scarring |
US20160193582A1 (en) | 2013-07-18 | 2016-07-07 | The Governors Of The University Of Alberta | Parallel organic synthesis on patterned paper using a solvent-repelling material |
CN103422379B (zh) * | 2013-08-19 | 2016-03-30 | 南京林业大学 | 一种甘蔗渣纤维素纳米纤维膜的制备方法 |
FI126854B (en) * | 2013-12-30 | 2017-06-30 | Upm Kymmene Corp | The membrane, its use and the method for making the membrane |
CA2937801C (en) * | 2014-01-23 | 2023-04-04 | Nissan Chemical Industries, Ltd. | Liquid culture medium composition comprising dispersed chitin or chitosan nanofibers |
WO2015111734A1 (ja) * | 2014-01-23 | 2015-07-30 | 日産化学工業株式会社 | 未分化性維持培養材料 |
JP6369044B2 (ja) * | 2014-02-19 | 2018-08-08 | 凸版印刷株式会社 | 培養装置およびその製造方法 |
JP2015159778A (ja) * | 2014-02-28 | 2015-09-07 | 日本バイリーン株式会社 | 光学的測定用細胞培養担体、光学的測定用ウェルプレート、及び細胞の光学的測定方法 |
WO2015136370A2 (en) * | 2014-03-10 | 2015-09-17 | 3-D Matrix, Ltd. | Sterilization and filtration of peptide compositions |
CN106456837B (zh) * | 2014-03-21 | 2020-04-10 | 匹兹堡大学-联邦高等教育体系 | 最终消毒的来自细胞外基质的水凝胶的制备方法 |
JP5727657B1 (ja) * | 2014-09-22 | 2015-06-03 | 第一工業製薬株式会社 | セルロースナノファイバー分散体の製造方法。 |
WO2016100856A1 (en) | 2014-12-18 | 2016-06-23 | Advanced Polymer Technology Ab | Cellulose nanofibrillar bionik for 3d bioprinting for cell culturing, tissue engineering and regenerative medicine applications |
FI126398B (en) | 2014-12-18 | 2016-11-15 | Upm Kymmene Corp | Procedure for testing chemicals |
FI125884B2 (en) | 2014-12-22 | 2019-03-29 | Upm Kymmene Corp | Treatment of a high temperature nanofibrillar cellulose hydrogel |
FI125883B (en) | 2014-12-22 | 2016-03-31 | Upm Kymmene Corp | Treatment of Catalytically Oxidized Nanofibril Cellulose Hydrogel |
FI126120B (en) * | 2014-12-22 | 2016-06-30 | Upm Kymmene Corp | Process for preparation of oxidized hydrogel of nanofibrillar cellulose |
FI125758B (en) * | 2014-12-22 | 2016-02-15 | Upm Kymmene Corp | A method for reducing the viscosity of nanofibrillar cellulose hydrogel |
WO2016146616A1 (en) | 2015-03-16 | 2016-09-22 | Ventana Medical Systems, Inc. | Control slides for immunohistochemistry generated from three-dimensional cell line cultures |
US11390835B2 (en) * | 2015-05-08 | 2022-07-19 | University Of Florida Research Foundation, Inc. | Growth media for three-dimensional cell culture |
FI127834B (en) | 2015-06-04 | 2019-03-29 | Upm Kymmene Corp | Process for the production of nanofibril cellulose hydrogel |
AU2016315779B2 (en) * | 2015-08-31 | 2022-04-21 | Cormedix Inc. | Delivery of active agents using nanofiber webs |
EP3232432A1 (de) * | 2016-04-11 | 2017-10-18 | EMPA Eidgenössische Materialprüfungs- und Forschungsanstalt | Verfahren zur verbesserung der akustischen eigenschaften von fichten-klangholz |
JP2019502165A (ja) | 2015-12-30 | 2019-01-24 | エーエムペーアー・アイトゲネーシッシェ・マテリアルプリューフングス‐ウント・フォルシュングスアンシュタルト | スプルース共鳴木材の音響特性を改善する方法 |
JP2017127264A (ja) * | 2016-01-21 | 2017-07-27 | 株式会社スギノマシン | アスペルギルス・オリゼ由来のホルムアルデヒド分解酵素およびホルムアルデヒド分解酵素を用いたホルムアルデヒドの分解方法 |
JP2017189164A (ja) * | 2016-04-08 | 2017-10-19 | 三菱ケミカル株式会社 | 細胞及び/又は細胞外マトリックス成分を含む組成物 |
JPWO2017199737A1 (ja) * | 2016-05-16 | 2019-03-07 | 富士フイルム株式会社 | 培養細胞の回収方法および培養細胞分散液 |
US20190209738A1 (en) * | 2016-06-09 | 2019-07-11 | Paul Gatenholm | Preparation and applications of modified cellulose nanofibrils with extracellular matrix components as 3d bioprinting bioinks to control cellular fate processes such as adhesion, proliferation and differentiation |
WO2018074432A1 (ja) * | 2016-10-17 | 2018-04-26 | 株式会社日本触媒 | 細胞培養用基材およびその製造方法、ならびにこれを利用した細胞培養容器および細胞培養方法 |
JP6874329B2 (ja) * | 2016-11-01 | 2021-05-19 | 凸版印刷株式会社 | ホルムアルデヒド除去組成物およびその製造方法、ホルムアルデヒド除去シート |
CN106544281B (zh) * | 2016-12-07 | 2019-07-02 | 哈尔滨工业大学 | 一种基于蛋白质和多糖构筑生物体保护层的制备方法 |
EP3335740B1 (en) * | 2016-12-15 | 2024-07-24 | UPM-Kymmene Corporation | Medical hydrogel |
DE102017000368A1 (de) | 2017-01-16 | 2018-07-19 | Gabriele Blume | Einbringen von schwer und/oder nicht wasserlöslichen Wirkstoffen mittels auf Lipiden basierenden Nanopartikeln/Vesikeln in ein hydrophiles aus Cellulose bestehendes dreidimensionales Netzwerk. |
EP3597729A4 (en) * | 2017-03-30 | 2020-04-08 | Nissan Chemical Corporation | CELL CULTURE USING NANO FIBERS |
SG11201909419XA (en) | 2017-04-10 | 2019-11-28 | TheWell Bioscience | Hydrogel for cell culture and biomedical applications |
EP3418377B1 (en) | 2017-06-22 | 2024-04-17 | UPM-Kymmene Corporation | Supportive nanofibrillar cellulose scaffold for expanding cells |
JP7132566B2 (ja) * | 2017-09-04 | 2022-09-07 | 学校法人早稲田大学 | チキソトロピー性を有するゲルを用いる多層3次元細胞培養足場システム |
CN108300713A (zh) * | 2017-12-31 | 2018-07-20 | 宁波大学 | 固定细胞的方法及装置 |
WO2019145795A2 (en) | 2018-01-26 | 2019-08-01 | Cellink Ab | Systems and methods for optical assessments of bioink printability |
SI3533458T1 (sl) * | 2018-03-02 | 2023-09-29 | Upm-Kymmene Corporation | Medicinski izdelek, ki vsebuje bioaktivno molekulo, ki je imobilizirana na nanofibrilarno celulozo in postopek za njegovo pripravo |
WO2019181294A1 (ja) * | 2018-03-23 | 2019-09-26 | 国立研究開発法人物質・材料研究機構 | スルホン化セルロースナノファイバーを含むハイドロゲル |
EP3581591A1 (en) | 2018-06-13 | 2019-12-18 | UPM-Kymmene Corporation | A nanofibrillar cellulose product and a method for manufacturing thereof |
EP3616730A1 (en) * | 2018-08-28 | 2020-03-04 | UPM-Kymmene Corporation | Composition or matrix for storage of bacteriophages comprising nanofibrillar cellulose |
US11186736B2 (en) | 2018-10-10 | 2021-11-30 | Cellink Ab | Double network bioinks |
EP3750982A1 (en) * | 2019-06-10 | 2020-12-16 | UPM-Kymmene Corporation | Cell culture plate, method for preparing thereof and method for detecting a substance |
HUP1900226A1 (hu) * | 2019-06-21 | 2020-12-28 | Hummel Zoltan Dr | Hidrofil mikrobiális cellulóz nanorostokat tartalmazó hordozóanyag (gél) a bélnyálkahártya állapotának javítására |
CN110387060B (zh) * | 2019-07-15 | 2021-10-29 | 陕西科技大学 | 一种用于细胞培养的水下透明多孔纤维素纸基材料及其制备方法和应用 |
EP3771470A1 (en) * | 2019-07-29 | 2021-02-03 | UPM-Kymmene Corporation | Medical hydrogel comprising nanofibrillar cellulose, tailored wound dressing, and methods for preparing thereof |
US11826951B2 (en) | 2019-09-06 | 2023-11-28 | Cellink Ab | Temperature-controlled multi-material overprinting |
EP3791864A1 (en) | 2019-09-13 | 2021-03-17 | UPM-Kymmene Corporation | Method for preparing pharmaceutical composition and pharmaceutical composition |
US11131059B2 (en) * | 2019-11-15 | 2021-09-28 | Innovatech Engineering Llc | Nanocellulose composite sheet for use as dermatological treatment or medical device |
US20220295861A1 (en) * | 2019-12-09 | 2022-09-22 | Nicoventures Trading Limited | Oral composition with nanocrystalline cellulose |
US11969502B2 (en) | 2019-12-09 | 2024-04-30 | Nicoventures Trading Limited | Oral products |
US11793230B2 (en) | 2019-12-09 | 2023-10-24 | Nicoventures Trading Limited | Oral products with improved binding of active ingredients |
US11826462B2 (en) | 2019-12-09 | 2023-11-28 | Nicoventures Trading Limited | Oral product with sustained flavor release |
US20210170031A1 (en) * | 2019-12-09 | 2021-06-10 | Nicoventures Trading Limited | Oral composition with nanocrystalline cellulose |
US11872231B2 (en) | 2019-12-09 | 2024-01-16 | Nicoventures Trading Limited | Moist oral product comprising an active ingredient |
GB2592240B (en) | 2020-02-21 | 2022-03-23 | Advanced Biofuel Solutions Ltd | Waste processing system |
JP2021136912A (ja) * | 2020-03-04 | 2021-09-16 | 国立大学法人東海国立大学機構 | 細胞の機能を変化させる方法 |
JP7502888B2 (ja) | 2020-04-20 | 2024-06-19 | 三洋化成工業株式会社 | 細胞培養基材用コーティング剤、細胞培養基材、細胞培養用キット及び細胞シートの生産方法 |
WO2022023815A1 (en) * | 2020-07-31 | 2022-02-03 | Ocean Tunicell As | Biocompatible, injectable and in situ gelling hydrogels and preparation and applications of biocompatible, injectable and in situ gelling hydrogels based on cellulose nanofibrils for tissue and organ repair |
CN112138215B (zh) * | 2020-09-26 | 2021-11-05 | 江苏大学 | 基于纳米纤维素的细胞生长因子缓释各向异性支架构建方法和应用 |
WO2023028664A1 (en) * | 2021-09-02 | 2023-03-09 | The University Of Queensland | Injectable composition |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008001728A (ja) * | 2006-06-20 | 2008-01-10 | Asahi Kasei Corp | 微細セルロース繊維 |
JP2009126837A (ja) * | 2007-11-27 | 2009-06-11 | Kao Corp | 食後gip及び/又は食後インスリン分泌抑制剤 |
JP2009523849A (ja) * | 2005-12-06 | 2009-06-25 | アルバート ミラニアン | セルロースゲル配合物 |
WO2009126106A1 (en) * | 2008-04-10 | 2009-10-15 | Stfi-Packforsk Ab | Method for providing a nanocellulose involving modifying cellulose fibers |
WO2010092239A1 (en) * | 2009-02-13 | 2010-08-19 | Upm-Kymmene Oyj | A method for producing modified cellulose |
WO2010115785A1 (en) * | 2009-03-30 | 2010-10-14 | Omya Development Ag | Process for the production of nano-fibrillar cellulose gels |
JP2011057746A (ja) * | 2009-09-07 | 2011-03-24 | Dai Ichi Kogyo Seiyaku Co Ltd | ゲル状組成物 |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0243151B1 (en) | 1986-04-22 | 1992-12-16 | Ajinomoto Co., Inc. | Modified microbially-produced cellulose gel and complex thereof with animal cell |
US5254471A (en) | 1986-10-06 | 1993-10-19 | Toray Industries, Inc. | Carrier for cell culture |
US5405953A (en) | 1993-08-03 | 1995-04-11 | Biocontrol Incorporated | Microfibrillated oxycellulose |
US6602994B1 (en) | 1999-02-10 | 2003-08-05 | Hercules Incorporated | Derivatized microfibrillar polysaccharide |
US7449180B2 (en) | 2001-02-06 | 2008-11-11 | John Kisiday | Macroscopic scaffold containing amphiphilic peptides encapsulating cells |
WO2004007683A2 (en) | 2002-07-15 | 2004-01-22 | Massachusetts Institute Of Technology | Cellular reprogramming in peptide hydrogel and uses thereof |
US20050037082A1 (en) | 2003-08-13 | 2005-02-17 | Wan-Kei Wan | Poly(vinyl alcohol)-bacterial cellulose nanocomposite |
US20080146701A1 (en) | 2003-10-22 | 2008-06-19 | Sain Mohini M | Manufacturing process of cellulose nanofibers from renewable feed stocks |
US7704740B2 (en) | 2003-11-05 | 2010-04-27 | Michigan State University | Nanofibrillar structure and applications including cell and tissue culture |
CN100531685C (zh) * | 2005-07-20 | 2009-08-26 | 同济大学 | 一种组织工程血管及其体外构建方法 |
WO2007012050A2 (en) | 2005-07-20 | 2007-01-25 | Surmodics, Inc. | Polymer coated nanofibrillar structures and methods for cell maintenance and differentiation |
CN100355975C (zh) * | 2005-08-19 | 2007-12-19 | 清华大学 | 一种天然纳米纤维的制备方法 |
US8951551B2 (en) | 2005-08-31 | 2015-02-10 | Board Of Regents, The University Of Texas System | Multiribbon nanocellulose as a matrix for wound healing |
US20070275458A1 (en) | 2005-12-09 | 2007-11-29 | The Research Foundation Of State University Of New York | Three dimensional-BIO-mimicking active scaffolds |
US8450105B2 (en) | 2006-05-04 | 2013-05-28 | Agency For Science, Technology And Research | Mechanically reversible gel |
US20080193536A1 (en) * | 2006-08-14 | 2008-08-14 | Alireza Khademhosseini | Cell-Laden Hydrogels |
JP5030667B2 (ja) * | 2007-05-29 | 2012-09-19 | 国立大学法人京都大学 | ミクロフィブリル化セルロース複合樹脂及びその製造方法 |
JP2008308802A (ja) * | 2007-06-18 | 2008-12-25 | Univ Of Tokyo | セルロースナノファイバーの製造方法 |
US8834917B2 (en) | 2007-11-13 | 2014-09-16 | Jawaharlal Nehru Centre For Advanced Scientific Research | Nanoparticle composition and process thereof |
CA2705369C (fr) | 2007-11-14 | 2016-10-18 | Ma.I.A Woundcare | Biomateriau permettant la delivrance controlee d'actifs |
DE102008014735A1 (de) | 2008-03-18 | 2009-09-24 | Studiengesellschaft Kohle Mbh | Verfahren zur Depolymerisation von Zellulose |
AT506657A1 (de) | 2008-04-14 | 2009-10-15 | Chemiefaser Lenzing Ag | Cellulosebasiertes hydrogel und verfahren zu seiner herstellung |
JP5386866B2 (ja) * | 2008-06-30 | 2014-01-15 | 国立大学法人京都大学 | ナノファイバーシート |
JP2010013604A (ja) | 2008-07-07 | 2010-01-21 | Toray Ind Inc | セルロース分散体 |
US20100065236A1 (en) * | 2008-09-17 | 2010-03-18 | Marielle Henriksson | Method of producing and the use of microfibrillated paper |
US9861096B2 (en) | 2008-10-01 | 2018-01-09 | Cornell University | Biodegradable chemical delivery system |
CN104888620B (zh) * | 2008-10-07 | 2017-09-15 | 纽约州立大学研究基金会 | 高通量高效率纳米纤维膜及其制备方法 |
CN101392246A (zh) * | 2008-10-29 | 2009-03-25 | 东北电力大学 | 一种利用细菌纤维素膜做载体固定化白腐真菌的方法 |
US20100172889A1 (en) | 2008-12-05 | 2010-07-08 | Catchmark Jeffrey M | Degradable biomolecule compositions |
US9192655B2 (en) * | 2009-03-12 | 2015-11-24 | New Jersey Institute Of Technology | System and method for a hydrogel and hydrogel composite for cartilage repair applications |
CA2786141A1 (en) | 2009-12-30 | 2011-07-07 | Axcelon Biopolymers Corporation | Transparent bacterial cellulose nanocomposite hydrogels |
FI123988B (fi) | 2010-10-27 | 2014-01-31 | Upm Kymmene Corp | Soluviljelymateriaali |
-
2010
- 2010-10-27 FI FI20106121A patent/FI123988B/fi active IP Right Grant
-
2011
- 2011-10-26 US US13/881,973 patent/US20140010790A1/en not_active Abandoned
- 2011-10-26 PL PL15155375T patent/PL2975115T3/pl unknown
- 2011-10-26 US US13/881,982 patent/US9631177B2/en active Active
- 2011-10-26 ES ES15155375T patent/ES2759257T3/es active Active
- 2011-10-26 AU AU2011322363A patent/AU2011322363B2/en active Active
- 2011-10-26 ES ES11785715.1T patent/ES2565958T3/es active Active
- 2011-10-26 RU RU2013122757/10A patent/RU2597978C2/ru active
- 2011-10-26 DK DK11785713.6T patent/DK2633032T3/en active
- 2011-10-26 SG SG2013030721A patent/SG189966A1/en unknown
- 2011-10-26 US US13/881,959 patent/US10612003B2/en active Active
- 2011-10-26 DK DK11785715.1T patent/DK2632493T3/da active
- 2011-10-26 CN CN201710872909.XA patent/CN107557330B/zh active Active
- 2011-10-26 PL PL11785713T patent/PL2633032T3/pl unknown
- 2011-10-26 WO PCT/FI2011/050939 patent/WO2012056109A2/en active Application Filing
- 2011-10-26 JP JP2013535476A patent/JP6321961B2/ja active Active
- 2011-10-26 DK DK15155375T patent/DK2975115T3/da active
- 2011-10-26 EP EP15155375.7A patent/EP2975115B1/en active Active
- 2011-10-26 WO PCT/FI2011/050941 patent/WO2012056111A2/en active Application Filing
- 2011-10-26 JP JP2013535475A patent/JP2013541956A/ja active Pending
- 2011-10-26 EP EP11785713.6A patent/EP2633032B1/en active Active
- 2011-10-26 EP EP11785715.1A patent/EP2632493B1/en active Active
- 2011-10-26 BR BR112013010377-9A patent/BR112013010377B1/pt active IP Right Grant
- 2011-10-26 CN CN2011800627192A patent/CN103354834A/zh active Pending
- 2011-10-26 EP EP11785714.4A patent/EP2633033B1/en active Active
- 2011-10-26 ES ES11785713.6T patent/ES2537268T3/es active Active
- 2011-10-26 CA CA2815276A patent/CA2815276C/en active Active
- 2011-10-26 WO PCT/FI2011/050940 patent/WO2012056110A2/en active Application Filing
-
2013
- 2013-04-24 IL IL225923A patent/IL225923B/en active IP Right Grant
-
2014
- 2014-02-19 HK HK14101542.5A patent/HK1188466A1/xx unknown
-
2017
- 2017-12-15 JP JP2017241022A patent/JP6448755B2/ja active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009523849A (ja) * | 2005-12-06 | 2009-06-25 | アルバート ミラニアン | セルロースゲル配合物 |
JP2008001728A (ja) * | 2006-06-20 | 2008-01-10 | Asahi Kasei Corp | 微細セルロース繊維 |
JP2009126837A (ja) * | 2007-11-27 | 2009-06-11 | Kao Corp | 食後gip及び/又は食後インスリン分泌抑制剤 |
WO2009126106A1 (en) * | 2008-04-10 | 2009-10-15 | Stfi-Packforsk Ab | Method for providing a nanocellulose involving modifying cellulose fibers |
WO2010092239A1 (en) * | 2009-02-13 | 2010-08-19 | Upm-Kymmene Oyj | A method for producing modified cellulose |
WO2010115785A1 (en) * | 2009-03-30 | 2010-10-14 | Omya Development Ag | Process for the production of nano-fibrillar cellulose gels |
JP2011057746A (ja) * | 2009-09-07 | 2011-03-24 | Dai Ichi Kogyo Seiyaku Co Ltd | ゲル状組成物 |
Non-Patent Citations (1)
Title |
---|
MUELLER ASTRID: "BACTERIAL NANOCELLULOSE WOUND DRESSING AS DRUG DELIVERY SYSTEM", ABSTRACTS OF PAPER; 239TH NATIONAL MEETING OF THE AMERICAN CHEMICAL SOCIETY, vol. V239, JPN5014000864, 21 March 2010 (2010-03-21), US, pages 22, ISSN: 0003148780 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2017532384A (ja) * | 2014-10-24 | 2017-11-02 | サントル ナスィオナル ド ラ ルシェルシュ スィアンティフィク(セ.エン.エル.エス.) | 時間制御型グルコース放出ヒドロゲル及びそれらの用途 |
JP2021507913A (ja) * | 2017-12-21 | 2021-02-25 | エイチ アンド エー ファーマケム カンパニー,リミテッドH&A Pharmachem Co., Ltd | 金属有機構造体及びナノセルロースを用いた経皮送達用複合体 |
JP7349156B2 (ja) | 2017-12-21 | 2023-09-22 | エイチ アンド エー ファーマケム カンパニー,リミテッド | 金属有機構造体及びナノセルロースを用いた経皮送達用複合体 |
JP2022530057A (ja) * | 2019-04-22 | 2022-06-27 | イーエルシー マネージメント エルエルシー | セルロースナノファイバーを含有する局所送達系 |
JP2022538278A (ja) * | 2019-06-24 | 2022-09-01 | ユー ピー エム キュンメネ コーポレーション | ナノフィブリルセルロースヒドロゲル中に真核細胞を含む移植可能な細胞組成物、その調製方法、及びナノフィブリルセルロースの使用 |
JP2021046393A (ja) * | 2019-09-13 | 2021-03-25 | ユー ピー エム キュンメネ コーポレーション | 注射可能な医薬製剤 |
JP7069484B2 (ja) | 2019-09-13 | 2022-05-18 | ユー ピー エム キュンメネ コーポレーション | 注射可能な医薬製剤 |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6321961B2 (ja) | 薬剤送達組成物 | |
Dutta et al. | Functional cellulose-based hydrogels as extracellular matrices for tissue engineering | |
Abeer et al. | A review of bacterial cellulose-based drug delivery systems: their biochemistry, current approaches and future prospects | |
JP6576933B2 (ja) | パターニングされたメンブレン | |
Qian et al. | The state-of-the-art application of functional bacterial cellulose-based materials in biomedical fields | |
JP6441680B2 (ja) | グラム陽性菌の微生物培養のためのマトリックス及び組成物 | |
FI123715B (en) | Composition for embedded microbial culture | |
JP2020524507A (ja) | 増殖細胞のための支持的ナノフィブリルセルロース足場 | |
Munarin et al. | Structural properties of polysaccharide-based microcapsules for soft tissue regeneration |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A529 | Written submission of copy of amendment under article 34 pct |
Free format text: JAPANESE INTERMEDIATE CODE: A529 Effective date: 20130618 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20141024 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150908 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20151208 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160106 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20160517 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160915 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20161027 |
|
A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20161122 |
|
A912 | Re-examination (zenchi) completed and case transferred to appeal board |
Free format text: JAPANESE INTERMEDIATE CODE: A912 Effective date: 20161222 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20180104 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180202 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20180309 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20180406 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6321961 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |