JP2011074047A - 腐植抽出液の製造方法 - Google Patents
腐植抽出液の製造方法 Download PDFInfo
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- JP2011074047A JP2011074047A JP2009230184A JP2009230184A JP2011074047A JP 2011074047 A JP2011074047 A JP 2011074047A JP 2009230184 A JP2009230184 A JP 2009230184A JP 2009230184 A JP2009230184 A JP 2009230184A JP 2011074047 A JP2011074047 A JP 2011074047A
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- humus
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- humus extract
- bacteria
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- 238000000034 method Methods 0.000 title abstract description 15
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- AWYFVXROQOFXKI-UHFFFAOYSA-J calcium magnesium tetraacetate hydrate Chemical compound O.C(C)(=O)[O-].[Ca+2].[Mg+2].C(C)(=O)[O-].C(C)(=O)[O-].C(C)(=O)[O-] AWYFVXROQOFXKI-UHFFFAOYSA-J 0.000 claims abstract description 5
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- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
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- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Medicines Containing Plant Substances (AREA)
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Abstract
【解決手段】本発明の腐植抽出液の製造方法は、(1)腐植土を含水率50〜80%に保って明反応させ、(2)得られる腐植を、水および酢酸マグネシウムカルシウム水和物と混合し、発酵および熟成させることからなる。工程(2)で腐植に、さらにスキムミルクや軽石を混合することを好ましい。
【選択図】なし
Description
(1)pH緩衝作用が大きい、
(2)キレート反応をする、
(3)陽イオン交換容量が高い、
(4)土壌の団粒構造を形成する、
(5)生理活性機能がある、
(6)病原菌を抑制する、
(7)動植物の生育障害防止作用がある、
(8)脱臭作用がある、
(9)汚水の清浄作用がある、
(10)汚水処理工程で汚泥削減効果がある、
(11)抗酸化作用がある。
2)腐植抽出液の殺菌は、121℃、15分の熱殺菌とした。
(1)腐植土を含水率50〜80%に保って明反応させ、
(2)得られる腐植に、水および酢酸マグネシウムカルシウム水和物を混合し、発酵および熟成させることからなる。その詳細を以下に説明する。
〔実施例1〕
(工程1)
エンザイム株式会社長崎工場敷地内で約8000年間地下に埋蔵されていた腐植土を掘削し、太陽光の下、常温で約12ヶ月間、明反応を行った。この明反応により、酵素および補酵素の含有量を高めた腐植の物性を表3に示す。
工程1で得られた腐植と水とを重量比30:70の割合で混合した(腐植の含水率は50%となる)。その混合物に栄養剤として市販のスキンミルク100ppm、生理活性化剤としてCMA100ppm、微生物増殖用支持体として軽石0.1重量%を添加して、常温で3〜12ヶ月間発酵を熟成した。熟成から2〜11ヶ月間は曝気と槽内攪拌を続け、そして残る1ヶ月間は静止沈降させた。上澄液をろ紙またはカセットフィルターでろ過して、腐植抽出液を得た。腐植抽出液の物性を表4に示す。
比較(ポジティブコントロール)のために、エンザイム株式会社長崎工場敷地内で得た腐植土を用いて特開2006−273734に記載の製造方法に従って腐植抽出液を調製した。具体的には、実施例1の工程(2)において、スキンミルク、CMA、および軽石を混合しなかった以外は、実施例1と同様の手順で、腐植抽出液を調製した。この腐植抽出液の成分を表5に示す。表5の有機物、無機物ともに含有量は少量であるが、腐植抽出液は、腐植成分、ミネラル、微生物、酵素、補酵素が含まれた活性化された液といえる。
実施例1および比較例1の腐植抽出液に、大腸菌、緑膿菌、サルモネラ菌または黄色ブドウ球菌の菌液を接種後、25℃で保存し、60秒、6時間、および、24時間後、それぞれ、試験液中の生菌数を測定した。その結果を表6に示す。また、比較(ネガティブコントロール)として、試験液を精製水に変えた試験を追加した。
2)開始時:菌液接種直後の対照の生菌数を測定し、開始時とした。
この試験に先立ち予備試験を行ない、ウイルス感染価の測定法について検討した。実施例1の腐植抽出液の原液では十分な不活性化が予測できたので、注射用水を用いて腐植抽出液の5倍液を調製して、本試験を行なうことにした。さらに、細胞維持培地で作用液を100倍に希釈して感染価を測定した。また、比較(ネガティブコントロール)として、試験液を精製水に変えた試験を追加した。
<2.5:検出せず
logTCID50 :作用液1mlあたりのTCID50の対数値
1)開始時:作用開始直後の対照のTCID50を測定し、開始時とした。
2)精製水:ネガティブコントロール
Claims (3)
- 以下の工程:
(1)腐植土を含水率50〜80%に保って明反応させ、
(2)得られる腐植を、水および酢酸マグネシウムカルシウム水和物と混合し、発酵および熟成させる
ことからなる、腐植抽出液の製造方法。 - 工程(2)で、前記腐植に、さらにスキムミルクを混合することを特徴とする、請求項1に記載の腐植の製造方法。
- 工程(2)で、前記腐植に、さらに軽石を混合することを特徴とする、請求項1または2に記載の腐植の製造方法。
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013043988A (ja) * | 2011-08-26 | 2013-03-04 | Toshi Kakugyo Kk | 尿石除去剤 |
WO2013065439A1 (ja) * | 2011-11-01 | 2013-05-10 | 味の素株式会社 | 植物ウイルスの感染抑制剤およびそれを用いた植物ウイルス感染抑制方法 |
JP2020048468A (ja) * | 2018-09-26 | 2020-04-02 | エンザイム株式会社 | 水産動物の養殖方法 |
WO2024080322A1 (ja) * | 2022-10-13 | 2024-04-18 | 日本製紙株式会社 | 抗ホワイトスポットシンドロームウイルス剤 |
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JPH1059861A (ja) * | 1996-08-15 | 1998-03-03 | Keinzu Corp:Kk | 殺菌剤 |
JP2000136140A (ja) * | 1998-10-29 | 2000-05-16 | Ra Purata Koeki Kk | 腐植土抽出物質含有水性液 |
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JPS56167610A (en) * | 1980-05-28 | 1981-12-23 | Yoshihiko Takeshita | Germicide comprising extracted solution of humic acid |
JPS61112022A (ja) * | 1984-05-07 | 1986-05-30 | Mamoru Uchimizu | 腐植化反応を利用した抗菌性製剤 |
JPH02292385A (ja) * | 1989-03-06 | 1990-12-03 | Chevron Res Co | 酢酸マグネシウムカルシウム及びキレート化剤を含む除氷組成物 |
JPH1059861A (ja) * | 1996-08-15 | 1998-03-03 | Keinzu Corp:Kk | 殺菌剤 |
JP2000136140A (ja) * | 1998-10-29 | 2000-05-16 | Ra Purata Koeki Kk | 腐植土抽出物質含有水性液 |
JP2006273734A (ja) * | 2005-03-29 | 2006-10-12 | Enzyme Kk | 腐植、腐植抽出液および保湿液、ならびにそれらの製造方法および使用 |
JP2008007451A (ja) * | 2006-06-28 | 2008-01-17 | Ray & Company Inc | 殺菌剤 |
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JP2013043988A (ja) * | 2011-08-26 | 2013-03-04 | Toshi Kakugyo Kk | 尿石除去剤 |
WO2013065439A1 (ja) * | 2011-11-01 | 2013-05-10 | 味の素株式会社 | 植物ウイルスの感染抑制剤およびそれを用いた植物ウイルス感染抑制方法 |
US10617122B2 (en) | 2011-11-01 | 2020-04-14 | Ajinomoto Co., Inc. | Plant virus infection inhibitor and a method for inhibiting plant virus infection using the same |
JP2020048468A (ja) * | 2018-09-26 | 2020-04-02 | エンザイム株式会社 | 水産動物の養殖方法 |
JP7169502B2 (ja) | 2018-09-26 | 2022-11-11 | エンザイム株式会社 | 水産動物の養殖方法 |
WO2024080322A1 (ja) * | 2022-10-13 | 2024-04-18 | 日本製紙株式会社 | 抗ホワイトスポットシンドロームウイルス剤 |
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