JP2011038922A - Light detection chip, and light detection device using the same - Google Patents

Light detection chip, and light detection device using the same Download PDF

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JP2011038922A
JP2011038922A JP2009187313A JP2009187313A JP2011038922A JP 2011038922 A JP2011038922 A JP 2011038922A JP 2009187313 A JP2009187313 A JP 2009187313A JP 2009187313 A JP2009187313 A JP 2009187313A JP 2011038922 A JP2011038922 A JP 2011038922A
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fluorescence
light detection
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Isao Ichimura
功 市村
Masanobu Yamamoto
眞伸 山本
Shinichi Kai
慎一 甲斐
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Sony Corp
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Priority to CN2010102464356A priority patent/CN101995397A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/168—Specific optical properties, e.g. reflective coatings

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Abstract

<P>PROBLEM TO BE SOLVED: To provide a technology for efficiently detecting light from a small quantity of a sample. <P>SOLUTION: The light detection chip 1 is provided, and includes: a plurality of detection areas 11 for detecting a fluorescence F emitted from the sample by irradiating the sample with light; and a light reflection means 12 disposed at a position at which it faces the direction D for detecting the fluorescence F through the detection areas 11. The light detection chip 1 includes the light reflection means 12 disposed at the position at which it faces the direction D for detecting the fluorescence F through the detection areas 11, thereby the fluorescence F among the fluorescence F emitted from the sample is scattered in the direction opposite to the direction for detecting the fluorescence F, and reflected and guided in the fluorescence detection direction. The light can efficiently be detected from the small quantity of the sample. The assay accuracy and the analysis accuracy can be improved. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

本発明は、光検出用チップに関する。より詳細には、遺伝子発現解析、感染症検査、またＳＮＰ解析等の遺伝子解析、タンパク質解析、細胞解析などに供せられる光検出用チップおよび該光検出用チップを用いた光検出装置に関する。   The present invention relates to a light detection chip. More specifically, the present invention relates to a photodetection chip used for gene expression analysis, infectious disease testing, gene analysis such as SNP analysis, protein analysis, cell analysis, and the like, and a photodetection device using the photodetection chip.

近年、医療分野、創薬分野、臨床検査分野、食品分野、農業分野、工学分野、法医学分野、犯罪鑑識分野などの様々な分野で、遺伝子解析、タンパク質解析、細胞解析などに関する技術研究が広く進められている。特に最近では、核酸やタンパク質、細胞などの検出や解析など各種の反応を、チップに設けられたマイクロスケールの流路やウエル内で行うラボ・オン・チップの技術開発や実用化が進められており、生体分子などを簡便に計測する手法として注目を集めている。   In recent years, research on gene analysis, protein analysis, cell analysis, etc. has been widely promoted in various fields such as medical field, drug discovery field, clinical inspection field, food field, agriculture field, engineering field, forensic field, criminal field. It has been. In recent years, the development and practical application of lab-on-chip technology has been promoted, in which various reactions such as detection and analysis of nucleic acids, proteins, cells, etc. are performed in micro-scale channels and wells provided on the chip. Therefore, it is attracting attention as a method for easily measuring biomolecules.

このようなチップに設けられたマイクロスケールの流路やウエル内で行うラボ・オン・チップの技術において、混合、反応、分離精製、検出などの対象となるサンプルは、非常に微量である。そのため、効率的に検出や解析を行うためには、用いるチップや検出・解析方法などに様々な工夫を行う必要がある。   In a lab-on-chip technique performed in a microscale flow path or well provided in such a chip, the amount of samples to be mixed, reacted, separated, purified, detected, etc. is very small. For this reason, in order to perform detection and analysis efficiently, it is necessary to devise various techniques for the chip to be used and the detection / analysis method.

例えば、特許文献１では、試薬が熱溶解性結合剤と混合されてマイクロ流路内の所定位置に担架され、被検査液の導入時温度からの昇温により熱溶解性結合剤が担架されている所定位置で溶解を開始することで、溶解処理、混合処理を効率よく実施でき、更に、その後の反応処理や分析処理を同一位置で実施可能なため、マイクロ流路上の処理位置の削減により、小型化や、製造コストの低減を図ることができるマイクロ流路チップが提案されている。   For example, in Patent Document 1, a reagent is mixed with a heat-soluble binder and suspended at a predetermined position in the microchannel, and the heat-soluble binder is stretched by raising the temperature from the introduction temperature of the liquid to be inspected. The dissolution process and mixing process can be carried out efficiently by starting the dissolution at a predetermined position, and the subsequent reaction process and analysis process can be carried out at the same position. A microchannel chip that can be reduced in size and reduced in manufacturing cost has been proposed.

特許文献２では、１）蛍光プローブ分子及び生分子を準備する工程、２）ラボオンアチップ（Lab−on−a−chip）のマイクロチャンネルに蛍光プローブ分子及び生分子を注入して複合体を形成させる工程、３）前記複合体に偏光を照射して蛍光偏光を測定する工程、及び４）前記蛍光偏光を定量化して蛍光偏光度を決定する工程を行うことで、既存の方法に比べて１／１００程度の少量の試料を使用した分析が可能な蛍光偏光測定方法が提案されている。   In Patent Document 2, 1) a step of preparing a fluorescent probe molecule and a biomolecule, 2) a fluorescent probe molecule and a biomolecule are injected into a microchannel of a lab-on-a-chip to form a complex. 3) irradiating the composite with polarized light and measuring fluorescence polarization; and 4) quantifying the fluorescence polarization and determining the fluorescence polarization degree. A method for measuring fluorescence polarization capable of analysis using a small amount of sample of about 100/100 has been proposed.

特許文献３では、第１の電極を具備する核酸調製部と、該核酸調製部に試料流体を流入するための試料流入部と、該核酸調製部と流路によって連通した第２の電極を具備する反応部と、該反応部に薬液を流入するための薬液流入部と、該反応部から流体を流出させる流出部と、前記第一及び第２の電極と接続した制御回路と、前記第２の電極と接続した検出回路とを、一つの基板１００上に具備することにより、核酸の複製や合成、反応及び検出等を、一つの基板上で行うことが可能なラボ・オン・チップが提案されている。   In Patent Document 3, a nucleic acid preparation unit including a first electrode, a sample inflow unit for allowing a sample fluid to flow into the nucleic acid preparation unit, and a second electrode communicated with the nucleic acid preparation unit by a flow path are provided. A reaction part that conducts, a chemical solution inflow part for allowing a chemical solution to flow into the reaction part, an outflow part that causes a fluid to flow out from the reaction part, a control circuit connected to the first and second electrodes, and the second Providing a lab-on-a-chip capable of performing nucleic acid replication, synthesis, reaction, detection, etc. on a single substrate by providing a detection circuit connected to the electrodes on a single substrate 100 Has been.

特許文献４では、作用電極、参照電極、及び対抗電極からなる検出電極と、薄膜トランジスタと、を備えることにより、軽薄短小、高性能、かつ低コストのバイオセンシングデバイスを実現可能であり、インクジェットヘッド部を備えたバイオセンサに着脱可能なバイオチップが提案されている。   In Patent Document 4, a thin, thin, high-performance, and low-cost biosensing device can be realized by including a detection electrode composed of a working electrode, a reference electrode, and a counter electrode, and a thin film transistor. There has been proposed a biochip that can be attached to and detached from a biosensor equipped with the above.

特開２００８−１５１７７０号公報JP 2008-151770 A 特開２００７−１３９７４４号公報JP 2007-139744 A 特開２００８−１７７７９号公報JP 2008-17779 A 特開２００７−１８７５８２号公報JP 2007-187582 A

サンプルからの各種検出などは、ラボ・オン・チップ内に導入されたサンプルへ光照射を行うことにより、サンプル中から発せられた蛍光などの光を検出することで行われるのが一般的である。前述の通り、ラボ・オン・チップの技術分野においては、様々な工夫がなされているが、検出効率を高めて分析・解析精度を向上させるためには、微量なサンプルからの光検出を、如何に効率的に行うかが重要である。   Various types of detection from samples are generally performed by detecting light such as fluorescence emitted from the sample by irradiating the sample introduced into the lab-on-chip with light. . As described above, various efforts have been made in the technical field of lab-on-a-chip, but in order to improve the detection efficiency and improve the analysis and analysis accuracy, how to detect light from a very small amount of sample? It is important to do it efficiently.

しかしながら、サンプルから発せられる蛍光などの光は、３６０度方向に発せられるため、微量なサンプルから効率的に光を検出することは難しい場合が多い。また、励起光やサンプル中に含まれる物質から発せられる自家蛍光などの影響により、目的の蛍光のみを効率的に検出することは難しい。実際、一般的な光検出装置においては、サンプルから発せられる蛍光のうち、約５％程度しか検出できないのが実情である。   However, since light such as fluorescence emitted from a sample is emitted in a 360-degree direction, it is often difficult to efficiently detect light from a small amount of sample. Moreover, it is difficult to efficiently detect only target fluorescence due to the influence of excitation light or autofluorescence emitted from a substance contained in a sample. Actually, in a general light detection apparatus, only about 5% of fluorescence emitted from a sample can be detected.

そこで、本発明では、微量なサンプルから効率的に光を検出することが可能な技術を提供することを主目的とする。   Therefore, the main object of the present invention is to provide a technique capable of efficiently detecting light from a very small amount of sample.

本願発明者らは、前記課題を解決するために鋭意研究を行った結果、光検出に用いるためのチップの構成に着目することにより、微量なサンプルからの効率的な光検出に成功し、本発明を完成させるに至った。   As a result of earnest research to solve the above problems, the present inventors have succeeded in efficient light detection from a very small amount of sample by paying attention to the configuration of a chip for use in light detection. The invention has been completed.

即ち、本発明では、まず、サンプルへの光照射によってサンプル中から発せられる蛍光の検出が行われる複数の検出領域と、
該検出領域を介して、前記蛍光を検出する方向と対向する位置に配置された光反射手段と、
が少なくとも備えられた光検出用チップを提供する。
本発明に係る光検出用チップは、検出領域を介して蛍光を検出する方向と対向する位置に光反射手段を有しているため、サンプルから発せられた蛍光のうち、蛍光を検出する方向と逆方向へ散乱する蛍光を反射させて、蛍光検出方向へと導くことが可能である。
本発明に係る光検出用チップに備える光反射手段は、サンプルから発せられた蛍光を反射させることができればその具体的構成は特に限定されないが、例えば、複数の前記検出領域に対して複数配置することも可能である。
本発明に係る光検出用チップに用いることができる光反射手段の具体的形状は特に限定されないが、例えば、凹面形状または平面形状を有する光反射手段を用いることができる。
また、本発明に係る光検出用チップには、サンプルへ照射される光を透過し、サンプル中から発せられる蛍光を反射する光反射手段を用いることもできる。
更に、本発明に係る光検出用チップには、サンプルへ照射される光を透過せず、サンプル中から発せられる蛍光を透過する光透過制御手段を更に備えることも可能である。 That is, in the present invention, first, a plurality of detection regions in which fluorescence emitted from the sample by light irradiation to the sample is detected, and
A light reflecting means disposed at a position opposite to the direction of detecting the fluorescence via the detection region;
Is provided with at least a light detection chip.
Since the light detection chip according to the present invention has the light reflecting means at a position opposite to the direction in which the fluorescence is detected via the detection region, the direction of detecting the fluorescence out of the fluorescence emitted from the sample It is possible to reflect fluorescence scattered in the opposite direction and guide it in the fluorescence detection direction.
The light reflection means provided in the light detection chip according to the present invention is not particularly limited in its specific configuration as long as it can reflect the fluorescence emitted from the sample. For example, a plurality of light reflection means are arranged for a plurality of the detection regions. It is also possible.
Although the specific shape of the light reflection means that can be used in the light detection chip according to the present invention is not particularly limited, for example, a light reflection means having a concave shape or a planar shape can be used.
Further, the light detection chip according to the present invention can also use a light reflecting means that transmits light irradiated to the sample and reflects fluorescence emitted from the sample.
Furthermore, the light detection chip according to the present invention may further include a light transmission control unit that does not transmit the light irradiated to the sample but transmits the fluorescence emitted from the sample.

本発明では、次に、サンプルへの光照射を行う光照射手段と、
該光照射手段からの光照射によってサンプル中から発せられる蛍光の検出が行われる複数の検出領域と、
前記蛍光を検出する光検出手段と、
前記検出領域を介して前記光検出手段と対向する位置に配置された光反射手段と、
が少なくとも備えられた光検出装置を提供する。
本発明に係る光検出装置の光照射手段と検出領域の間には、集光レンズを備えることも可能である。
本発明に係る光検出装置には、光照射手段、光反射手段、光検出手段、集光レンズは、複数の検出領域に対して少なくとも一つ備えていればよいが、複数の検出領域に対して複数配置することも可能である。
本発明に係る光検出装置に用いることができる光反射手段の具体的形状は特に限定されないが、例えば、凹面形状または平面形状を有する光反射手段を用いることができる。
また、本発明に係る光検出装置には、サンプルへ照射される光を透過し、サンプル中から発せられる蛍光を反射する光反射手段を用いることもできる。
更に、本発明に係る光検出装置には、サンプルへ照射される光を透過せず、サンプル中から発せられる蛍光を透過する光透過制御手段を更に備えることも可能である。 In the present invention, next, light irradiation means for irradiating the sample with light,
A plurality of detection regions in which fluorescence emitted from the sample by light irradiation from the light irradiation means is detected;
A light detection means for detecting the fluorescence;
A light reflecting means disposed at a position facing the light detecting means via the detection region;
Is provided with at least.
A condensing lens may be provided between the light irradiation means and the detection region of the light detection device according to the present invention.
The light detection device according to the present invention may include at least one light irradiation means, light reflection means, light detection means, and condenser lens for a plurality of detection areas. It is also possible to arrange a plurality of them.
Although the specific shape of the light reflection means that can be used in the light detection device according to the present invention is not particularly limited, for example, a light reflection means having a concave shape or a planar shape can be used.
Moreover, the light detection device according to the present invention can use a light reflecting means that transmits the light irradiated to the sample and reflects the fluorescence emitted from the sample.
Furthermore, the light detection apparatus according to the present invention may further include a light transmission control unit that does not transmit the light irradiated to the sample but transmits the fluorescence emitted from the sample.

本発明に係る光検出用チップは、検出領域を介して蛍光を検出する方向と対向する位置に光反射手段を備えているため、サンプルから発せられた蛍光のうち、蛍光を検出する方向と逆方向へ散乱する蛍光を反射させて、蛍光検出方向へと導くことが可能である。そのため、微量なサンプルから効率的に光を検出することが可能であり、分析精度および解析精度を向上させることが実現できる。   Since the light detection chip according to the present invention includes the light reflecting means at a position opposite to the fluorescence detection direction via the detection region, the fluorescence detection direction is opposite to the fluorescence detection direction. It is possible to reflect the fluorescence scattered in the direction and guide it in the fluorescence detection direction. Therefore, it is possible to detect light efficiently from a very small amount of sample, and it is possible to improve analysis accuracy and analysis accuracy.

本発明に係る光検出用チップ１の第１実施形態を模式的に示す断面模式図である。1 is a schematic cross-sectional view schematically showing a first embodiment of a light detection chip 1 according to the present invention. 本発明に係る光検出用チップ１の第２実施形態を模式的に示す断面模式図である。It is a cross-sectional schematic diagram which shows typically 2nd Embodiment of the chip | tip 1 for light detection which concerns on this invention. 本発明に係る光検出用チップ１の第３実施形態を模式的に示す平面模式図である。It is a plane schematic diagram which shows typically 3rd Embodiment of the chip | tip 1 for light detection which concerns on this invention. 本発明に係る光検出用チップ１の第４実施形態を模式的に示す断面模式図である。It is a cross-sectional schematic diagram which shows typically 4th Embodiment of the chip | tip 1 for optical detection which concerns on this invention. 本発明に係る光検出用チップ１の第５実施形態を模式的に示す断面模式図である。It is a cross-sectional schematic diagram which shows typically 5th Embodiment of the chip | tip 1 for optical detection which concerns on this invention. 本発明に係る光検出用チップ１の第６実施形態を模式的に示す断面模式図である。It is a cross-sectional schematic diagram which shows typically 6th Embodiment of the chip | tip 1 for optical detection which concerns on this invention. 本発明に係る光検出用チップ１の第７実施形態を模式的に示す断面模式図である。It is a cross-sectional schematic diagram which shows typically 7th Embodiment of the chip | tip 1 for light detection which concerns on this invention. 本発明に係る光検出用チップ１の第８実施形態を模式的に示す断面模式図である。It is a cross-sectional schematic diagram which shows typically 8th Embodiment of the chip | tip 1 for light detection which concerns on this invention. 本発明に係る光検出装置１０の第１実施形態を模式的に示す模式概念図である。1 is a schematic conceptual diagram schematically showing a first embodiment of a light detection device 10 according to the present invention. 本発明に係る光検出装置１０の第２実施形態を模式的に示す模式概念図である。It is a schematic conceptual diagram which shows typically 2nd Embodiment of the photon detection apparatus 10 which concerns on this invention. 本発明に係る光検出装置１０の第３実施形態を模式的に示す模式概念図である。It is a schematic conceptual diagram which shows typically 3rd Embodiment of the photon detection apparatus 10 which concerns on this invention.

以下、本発明を実施するための好適な形態について図面を参照しながら説明する。以下に説明する実施形態は、本発明の代表的な実施形態の一例を示したものであり、これにより本発明の範囲が狭く解釈されることはない。なお、説明は以下の順序で行う。
１．光検出用チップ１
（１）検出領域１１
（２）光反射手段１２
（３）光透過制御手段１３
２．光検出装置１０
（１）光照射手段１０１
（２）光検出手段１０２
（３）集光レンズ１０３ａ、１０３ｂ
（４）光学フィルター１０４ａ、１０４ｂ
（５）アパーチャー１０５ａ、１０５ｂ、隔壁 DESCRIPTION OF EXEMPLARY EMBODIMENTS Hereinafter, preferred embodiments for carrying out the invention will be described with reference to the drawings. The embodiment described below shows an example of a typical embodiment of the present invention, and thus the scope of the present invention is not construed narrowly. The description will be given in the following order.
1. Photodetection chip 1
(1) Detection area 11
(2) Light reflecting means 12
(3) Light transmission control means 13
2. Photodetector 10
(1) Light irradiation means 101
(2) Light detection means 102
(3) Condensing lenses 103a and 103b
(4) Optical filters 104a and 104b
(5) Apertures 105a and 105b, partition walls

＜１．光検出用チップ１＞
図１は、本発明に係る光検出用チップ１の第１実施形態を模式的に示す断面模式図である。本発明に係る光検出用チップ１は、（１）検出領域１１と、（２）光反射手段１２と、を少なくとも備えるチップである。また、必要に応じて、（３）光透過制御手段１３を備えることも可能である。 <1. Photodetection chip 1>
FIG. 1 is a schematic cross-sectional view schematically showing a first embodiment of a light detection chip 1 according to the present invention. The light detection chip 1 according to the present invention is a chip including at least (1) a detection region 11 and (2) a light reflection means 12. Moreover, it is also possible to provide (3) light transmission control means 13 as needed.

本発明に係る光検出用チップ１を形成する素材は特に限定されず、通常、バイオアッセイ用チップなどの光検出用チップに用いることが可能な素材を自由に選択して用いることができる。本発明では特に、光検出用に用いるため、光透過性を有するポリカーボネート、ポリオレフィン系、シクロオレフィン系、アクリル系などのプラスチック樹脂、ＰＤＭＳ（polydimethylsiloxane）などのシリコン系樹脂、ガラス等の基板を用いることが好ましい。   The material for forming the light detection chip 1 according to the present invention is not particularly limited, and normally, a material that can be used for a light detection chip such as a bioassay chip can be freely selected and used. In the present invention, in particular, since it is used for light detection, a plastic resin such as polycarbonate, polyolefin, cycloolefin, acrylic, etc. having optical transparency, a silicon resin such as PDMS (polydimethylsiloxane), or a substrate such as glass is used. Is preferred.

以下、本発明に係る光検出用チップ１に設けられた各手段などについて、それぞれ詳細に説明する。   Hereinafter, each means provided in the photodetection chip 1 according to the present invention will be described in detail.

（１）検出領域１１
検出領域１１は、検出対象となるサンプルが存在し、該サンプルへの光照射（励起光Ｅ）によってサンプル中から発せられる蛍光Ｆの検出が行われる領域である。本発明に係る光検出用チップ１における検出領域１１は、サンプルへの光照射（励起光Ｅ）によってサンプル中から発せられる蛍光Ｆの検出が行うことができる領域であれば、その具体的構成は特に限定されないが、例えば、図１に示す第１実施形態のように、ウエルＷ中に検出領域１１を設けることができる。また、例えば、図２に示す第２実施形態のように、流路Ｃ中に検出領域１１を設けることも可能である。なお、図２に示す第２実施形態では、一つの流路Ｃ中に複数の検出領域１１を設けているが、これに限らず、一つの流路Ｃに対して一つの検出領域１１を設けることも自由である。 (1) Detection area 11
The detection region 11 is a region where there is a sample to be detected, and fluorescence F emitted from the sample is detected by light irradiation (excitation light E) to the sample. The specific configuration of the detection region 11 in the light detection chip 1 according to the present invention is as long as the fluorescence F emitted from the sample can be detected by light irradiation (excitation light E) on the sample. Although not particularly limited, for example, the detection region 11 can be provided in the well W as in the first embodiment shown in FIG. Further, for example, the detection region 11 can be provided in the flow path C as in the second embodiment shown in FIG. In the second embodiment shown in FIG. 2, a plurality of detection regions 11 are provided in one channel C. However, the present invention is not limited to this, and one detection region 11 is provided for one channel C. It is also free.

また、図３に示す第３実施形態のように、基板Ｔ内にウエルＷと流路Ｃとを組み合わせて設け、ウエルＷ中や流路Ｃ中に検出領域１１（図示せず）を複数設けることも可能である。   Further, as in the third embodiment shown in FIG. 3, a well W and a flow path C are provided in combination in the substrate T, and a plurality of detection regions 11 (not shown) are provided in the well W and the flow path C. It is also possible.

検出領域１１を流路Ｃ中に設ける場合、流路Ｃの流路幅、流路深さ、流路断面形状も特に限定されず、自由に設計することができる。例えば、流路幅１ｍｍ以下のマイクロ流路なども、本発明に係る光検出用チップ１に用いることが可能である。   When the detection region 11 is provided in the channel C, the channel width, the channel depth, and the channel cross-sectional shape of the channel C are not particularly limited, and can be freely designed. For example, a microchannel having a channel width of 1 mm or less can also be used for the light detection chip 1 according to the present invention.

検出領域１１は、蛍光検出を行うだけでなく、例えば、核酸増幅、ハイブリダイゼーション、核酸、タンパク質、細胞などの物質間相互作用などが進行する反応場として用いることも可能である。また、図２に示す第２実施形態のように、流路Ｃ中に検出領域１１を設ける場合には、流路Ｃ中でサンプルを移動させながら各反応を進行させ、所定の位置に到達した時点で蛍光検出を行うことも可能である。また、図３に示す第３実施形態のように、基板Ｔ内にウエルＷと流路Ｃとを組み合わせて設ける場合、流路Ｃ中でサンプルを移動させながら各反応を進行させ、所定のウエルＷに到達した時点で蛍光検出を行ったり、ウエルＷ内で各反応を進行させ、流路Ｃ中でサンプルを移動させながら蛍光検出を行ったりすることも可能である。   The detection region 11 can be used not only for fluorescence detection but also as a reaction field in which, for example, nucleic acid amplification, hybridization, interaction between substances such as nucleic acids, proteins, and cells proceeds. When the detection region 11 is provided in the flow channel C as in the second embodiment shown in FIG. 2, each reaction is advanced while moving the sample in the flow channel C, and reaches a predetermined position. It is also possible to detect fluorescence at the time. When the well W and the flow path C are provided in combination in the substrate T as in the third embodiment shown in FIG. 3, each reaction is advanced while moving the sample in the flow path C, and a predetermined well. It is also possible to detect fluorescence when reaching W, or to detect fluorescence while moving each sample in the channel C by advancing each reaction in the well W.

なお、各検出領域１１へのサンプルの導入は、特に限定されず公知の方法を自由に用いることが可能である。例えば、図３に示す第３実施形態のように、基板Ｔ上に各ウエルＷへ通じる流路Ｃを形成し、流路Ｃを通じて各検出領域１１（図示せず）へサンプルを導入することができる。   The introduction of the sample into each detection region 11 is not particularly limited, and a known method can be used freely. For example, as in the third embodiment shown in FIG. 3, a channel C leading to each well W is formed on the substrate T, and a sample is introduced into each detection region 11 (not shown) through the channel C. it can.

（２）光反射手段１２
光反射手段１２は、サンプル中から発せられる蛍光Ｆを反射する手段であり、検出領域１１を介して、蛍光Ｆを検出する方向（蛍光検出方向Ｄ）と対向する位置に配置する。 (2) Light reflecting means 12
The light reflecting means 12 is a means for reflecting the fluorescence F emitted from the sample, and is disposed at a position facing the direction in which the fluorescence F is detected (fluorescence detection direction D) via the detection region 11.

サンプル中から発せられる蛍光Ｆは、３６０度方向に発せられるため、蛍光Ｆを取得する方向（蛍光検出方向Ｄ）と逆方向に発せられた光は、検出されることなく散乱してしまう。そのため、従来の光検出用チップを用いた光検出においては、微量なサンプルから効率的に光を検出することは難しかった。しかし、本発明に係る光検出用チップには、検出領域１１を介して蛍光Ｆを取得する方向（蛍光検出方向Ｄ）と対向する位置に、光反射手段１２を設けているため、蛍光Ｆを取得する方向（蛍光検出方向Ｄ）と逆方向に発せられた蛍光Ｆを反射させることにより、従来よりも多くの蛍光Ｆを検出することが可能である。その結果、微量なサンプルからも効率的に蛍光Ｆを検出することができ、分析精度および解析精度を向上させることができる。   Since the fluorescence F emitted from the sample is emitted in the direction of 360 degrees, the light emitted in the direction opposite to the direction in which the fluorescence F is acquired (fluorescence detection direction D) is scattered without being detected. Therefore, in light detection using a conventional light detection chip, it has been difficult to efficiently detect light from a small amount of sample. However, since the light detection chip according to the present invention is provided with the light reflecting means 12 at a position facing the direction in which the fluorescence F is acquired via the detection region 11 (fluorescence detection direction D), By reflecting the fluorescence F emitted in the direction opposite to the acquisition direction (fluorescence detection direction D), it is possible to detect more fluorescence F than before. As a result, the fluorescence F can be efficiently detected from a very small amount of sample, and the analysis accuracy and analysis accuracy can be improved.

本発明に係る光検出用チップ１に備える光反射手段１２は、サンプルから発せられた蛍光Ｆを反射させることができればその具体的構成は特に限定されず、自由に設計することができる。例えば、図１に示す第１実施形態や図２に示す第２実施形態のように、複数の検出領域１１に対して、複数の光反射手段１２を配置することが可能である。また、例えば、図４に示す第４実施形態のように、複数の検出領域１１に対して、１つの光反射手段１２を配置することも可能である。   The light reflecting means 12 included in the light detection chip 1 according to the present invention is not particularly limited as long as it can reflect the fluorescence F emitted from the sample, and can be freely designed. For example, as in the first embodiment shown in FIG. 1 and the second embodiment shown in FIG. 2, a plurality of light reflecting means 12 can be arranged for a plurality of detection regions 11. Further, for example, as in the fourth embodiment shown in FIG. 4, one light reflecting means 12 can be arranged for a plurality of detection regions 11.

本発明に係る光検出用チップ１に用いることができる光反射手段１２の具体的形状も、サンプルから発せられた蛍光Ｆを反射させることができれば特に限定されず、自由に設計することができる。例えば、図１に示す第１実施形態や図２に示す第２実施形態に用いている球面反射鏡や、図４に示す第４実施形態に用いている非球面反射鏡などの凹面形状のものを用いることができる。また、例えば、図５に示す第５実施形態のように、平面形状の反射鏡などを用いることもできる。   The specific shape of the light reflecting means 12 that can be used in the light detection chip 1 according to the present invention is not particularly limited as long as it can reflect the fluorescence F emitted from the sample, and can be freely designed. For example, concave-shaped ones such as the spherical reflector used in the first embodiment shown in FIG. 1 and the second embodiment shown in FIG. 2 and the aspheric reflector used in the fourth embodiment shown in FIG. Can be used. Further, for example, a planar reflecting mirror or the like can be used as in the fifth embodiment shown in FIG.

本発明に係る光検出用チップ１に用いる光反射手段１２として、サンプルへ照射される光（励起光Ｅ）は透過し、サンプル中から発せられる蛍光Ｆは反射するものを用いれば、図６示す第６実施形態のように、光照射と光検出を、逆方向から行うことが可能となる。このように、光照射と光検出を、検出領域１１を介して逆方向から行うことで、光照射に用いる光照射手段１０１や、蛍光検出に用いる光検出手段１０２（図示せず）を、より自由な構成で配置させることができる。   As the light reflecting means 12 used in the light detection chip 1 according to the present invention, FIG. 6 shows a case where light irradiated to the sample (excitation light E) is transmitted and fluorescence F emitted from the sample is reflected. As in the sixth embodiment, light irradiation and light detection can be performed from opposite directions. Thus, by performing light irradiation and light detection from the reverse direction through the detection region 11, the light irradiation means 101 used for light irradiation and the light detection means 102 (not shown) used for fluorescence detection are more It can be arranged in a free configuration.

（３）光透過制御手段１３
光透過制御手段１３は、サンプルへ照射される光（励起光Ｅ）は透過せず、サンプル中から発せられる蛍光Ｆは透過する手段である。本発明に係る光検出用チップ１には必須ではないが、光透過制御手段１３を設けることで、ＳＮ比をより向上させることが可能である。 (3) Light transmission control means 13
The light transmission control unit 13 is a unit that does not transmit the light (excitation light E) irradiated to the sample but transmits the fluorescence F emitted from the sample. Although not essential for the light detection chip 1 according to the present invention, by providing the light transmission control means 13, it is possible to further improve the SN ratio.

光透過制御手段１３の具体的な配置方法としては、例えば、図７に示す第７実施形態のように、光照射と光検出を同じ方向から行う場合には、検出領域１１と光反射手段１２との間に配置することが好ましい。また、例えば、図８に示す第８実施形態のように、光照射と光検出を、検出領域１１を介して逆方向から行う場合には、検出領域１１の蛍光検出方向側へ、光透過制御手段１３を配置することが好ましい。   As a specific arrangement method of the light transmission control means 13, for example, in the case where light irradiation and light detection are performed from the same direction as in the seventh embodiment shown in FIG. 7, the detection region 11 and the light reflection means 12 are used. Between the two. For example, as in the eighth embodiment shown in FIG. 8, when light irradiation and light detection are performed in the reverse direction via the detection region 11, light transmission control is performed toward the fluorescence detection direction side of the detection region 11. It is preferable to arrange the means 13.

このように光透過制御手段１３を配置することで、励起光Ｅの蛍光検出方向への散乱を防止することが可能である。その結果、励起光Ｅによるノイズを減少させることができ、ＳＮ比の向上を実現することができる。   By arranging the light transmission control means 13 in this way, it is possible to prevent scattering of the excitation light E in the fluorescence detection direction. As a result, noise due to the excitation light E can be reduced, and an improvement in the SN ratio can be realized.

＜２．光検出装置１０＞
図９は、本発明に係る光検出装置１０の第１実施形態を模式的に示す模式概念図である。本発明に係る光検出装置１０は、（１）光照射手段１０１と、検出領域１１と、（２）光検出手段１０２と、光反射手段と、を少なくとも備える装置である。また、必要に応じて、（３）集光レンズ１０３、（４）光学フィルター１０４、（５）アパーチャー１０５、隔壁などを備えることも可能である。以下、各構成について、詳細に説明する。なお、検出領域１１、光反射手段１２については、前述の光検出用チップ１と同様のため、ここでは説明を省略する。 <2. Photodetector 10>
FIG. 9 is a schematic conceptual view schematically showing the first embodiment of the photodetecting device 10 according to the present invention. The light detection apparatus 10 according to the present invention is an apparatus including at least (1) a light irradiation unit 101, a detection region 11, (2) a light detection unit 102, and a light reflection unit. Further, it is possible to provide (3) a condensing lens 103, (4) an optical filter 104, (5) an aperture 105, a partition wall, and the like as required. Hereinafter, each configuration will be described in detail. Since the detection region 11 and the light reflecting means 12 are the same as those of the above-described light detection chip 1, the description thereof is omitted here.

（１）光照射手段１０１
光照射手段１０１は、サンプルへ励起光Ｅを照射するための手段である。 (1) Light irradiation means 101
The light irradiation means 101 is means for irradiating the sample with excitation light E.

本発明に係る光検出装置１０において、光照射手段１０１の具体的配置方法は、サンプルへの光照射が可能であれば特に限定されず、自由に配置することが可能である。例えば、図９に示す第１実施形態のように、各検出領域１１に対応して複数の光照射手段１０１を配置することができる。このように複数の光照射手段１０１を配置することで、例えば、各検出領域１１に対して、それぞれ異なる波長の励起光Ｅを照射することで、同時に各種検出を行うことが可能である。   In the light detection apparatus 10 according to the present invention, the specific arrangement method of the light irradiation means 101 is not particularly limited as long as light irradiation to the sample is possible, and the light irradiation means 101 can be freely arranged. For example, as in the first embodiment shown in FIG. 9, a plurality of light irradiation means 101 can be arranged corresponding to each detection region 11. By arranging a plurality of light irradiation means 101 in this way, for example, various detections can be simultaneously performed by irradiating each detection region 11 with excitation light E having a different wavelength.

また、例えば、図示しないが、複数の検出領域１１に対して１つの光照射手段１０１を設け、光照射手段１０１を走査させることで、各検出領域１１に対して励起光Ｅを照射可能な構成にすることも可能である。   For example, although not shown, one light irradiation unit 101 is provided for a plurality of detection regions 11, and the excitation light E can be irradiated to each detection region 11 by scanning the light irradiation unit 101. It is also possible to make it.

本発明に係る光検出装置１０の光照射手段１０１に用いることができる光照射方法は、特に限定されず、公知の光照射方法を自由に選択して用いることができる。例えば、ＬＥＤ（Light Emitting Diode）、半導体レーザー、ＥＬ照明などを用いた光照射方法を１種または２種以上自由に選択して用いることが可能である。   The light irradiation method that can be used for the light irradiation means 101 of the light detection apparatus 10 according to the present invention is not particularly limited, and a known light irradiation method can be freely selected and used. For example, one or more light irradiation methods using LEDs (Light Emitting Diodes), semiconductor lasers, EL illumination, etc. can be freely selected and used.

光照射手段１０１を各検出領域１１に対応して複数配置する場合、光照射手段１０１での光照射は、一括で点灯させ、後述する光検出手段１０２を用いて一括で検出することにより、シグナル取得時間を短縮することができる。あるいは、各光照射手段１０１を、高速で順次点灯させることで、隣接する光照射手段１０１からのノイズを抑制することも可能である。   When a plurality of light irradiation means 101 are arranged corresponding to each detection region 11, the light irradiation by the light irradiation means 101 is turned on in a lump and is detected in a lump using the light detection means 102, which will be described later. Acquisition time can be shortened. Or it is also possible to suppress the noise from the adjacent light irradiation means 101 by lighting each light irradiation means 101 sequentially at high speed.

（２）光検出手段１０２
光検出手段１０２は、サンプル中から発せられる蛍光Ｆを検出するための手段である。 (2) Light detection means 102
The light detection means 102 is a means for detecting the fluorescence F emitted from the sample.

本発明に係る光検出装置１０において、光検出手段１０２の具体的配置方法は、サンプル中から発せられた蛍光Ｆの検出が可能であれば特に限定されず、自由に配置することが可能である。例えば、図９に示す第１実施形態のように、各検出領域１１に対応して複数の光検出手段１０２を配置することができる。このように複数の光検出手段１０２を配置することで、各検出領域１１に存在するサンプル中から発せられた蛍光Ｆを同時に検出することが可能である。   In the light detection apparatus 10 according to the present invention, the specific arrangement method of the light detection means 102 is not particularly limited as long as the fluorescence F emitted from the sample can be detected, and can be freely arranged. . For example, as in the first embodiment shown in FIG. 9, a plurality of light detection means 102 can be arranged corresponding to each detection region 11. By arranging a plurality of light detection means 102 in this way, it is possible to simultaneously detect the fluorescence F emitted from the sample existing in each detection region 11.

また、例えば、図示しないが、複数の検出領域１１に対して１つの光検出手段１０２を設け、光検出手段１０２を走査させることで、各検出領域１１に存在するサンプル中から発せられた蛍光Ｆを検出可能な構成にすることも可能である。   Further, for example, although not shown, the fluorescence F emitted from the sample existing in each detection region 11 by providing one light detection unit 102 for the plurality of detection regions 11 and scanning the light detection unit 102. It is also possible to make a configuration capable of detecting.

また、本発明に係る光検出装置１０では、光検出手段１０２を、検出領域１１を介して光照射手段１０１と対向する位置に配置することが好ましい。光照射手段１０１と光検出手段１０２を、検出領域１１を介して逆側に配置することで、光照射手段１０１や光検出手段１０２を、より自由な構成で配置させることができる。   In the light detection apparatus 10 according to the present invention, it is preferable that the light detection means 102 is disposed at a position facing the light irradiation means 101 through the detection region 11. By disposing the light irradiation unit 101 and the light detection unit 102 on the opposite side via the detection region 11, the light irradiation unit 101 and the light detection unit 102 can be arranged in a more free configuration.

本発明に係る光検出装置１０の光検出手段１０２に用いることができる検出方法は、特に限定されず、公知の光検出方法を自由に選択して用いることができる。例えば、ＰＤ（Photo Diode）、電荷結合素子（ＣＣＤ）、Complementary Metal Oxide Semiconductor（ＣＭＯＳ）、などのエリア撮像素子を用いた方法、複数の光検出器をアレイ状に並べた、いわゆるマルチチャンネル光検出器を用いた方法などを採用することができる。   The detection method that can be used for the light detection means 102 of the light detection device 10 according to the present invention is not particularly limited, and a known light detection method can be freely selected and used. For example, a method using an area imaging device such as a PD (Photo Diode), a charge coupled device (CCD), or a complementary metal oxide semiconductor (CMOS), a so-called multi-channel light detection in which a plurality of photodetectors are arranged in an array. A method using a vessel can be employed.

（３）集光レンズ１０３ａ、１０３ｂ
図９に示す第１実施形態では、光照射手段１０１からの光の集光のために、各光照射手段１０１と各検出領域１１との間に、複数の励起用集光レンズ１０３ａを配置している。本発明に係る光検出装置１０では、この励起用集光レンズ１０３ａは必須のものではないが、本実施形態のように励起用集光レンズ１０３ａを設ければ、検出領域１１内のサンプルに対して、より正確な光照射を行うことが可能である。 (3) Condensing lenses 103a and 103b
In the first embodiment shown in FIG. 9, a plurality of excitation condensing lenses 103 a are arranged between each light irradiation means 101 and each detection region 11 for condensing light from the light irradiation means 101. ing. In the light detection apparatus 10 according to the present invention, the excitation condensing lens 103a is not indispensable. However, if the excitation condensing lens 103a is provided as in the present embodiment, the sample in the detection region 11 is applied. Thus, more accurate light irradiation can be performed.

また、本実施形態では、各検出領域１１内のサンプル中から発せられた蛍光Ｆを光検出手段１０２に集光するために、各検出領域１１と各光検出手段１０２との間に、複数の受光用集光レンズ１０３ｂを配置している。本発明に係る光検出装置１０では、この受光用集光レンズ１０３ｂも必須のものではないが、本実施形態のように受光用集光レンズ１０３ｂを設ければ、蛍光Ｆ等のシグナルをより高めることができる。その結果、ＳＮ比の向上を図ることが可能である。   Further, in the present embodiment, in order to condense the fluorescence F emitted from the sample in each detection region 11 onto the light detection unit 102, a plurality of detection regions 11 are provided between each detection region 11 and each light detection unit 102. A light receiving condensing lens 103b is arranged. In the light detection device 10 according to the present invention, the light receiving condensing lens 103b is not indispensable, but if the light receiving condensing lens 103b is provided as in the present embodiment, the signal of the fluorescence F or the like is further increased. be able to. As a result, it is possible to improve the SN ratio.

（４）光学フィルター１０４ａ、１０４ｂ
図１０は、本発明に係る光検出装置１０の第２実施形態を模式的に示す模式概念図である。本実施形態では、各光照射手段１０１と各検出領域１１との間に、励起用光学フィルター１０４ａを設けている。本発明に係る光検出装置１０では、この励起用光学フィルター１０４ａは必須のものではないが、本実施形態のように励起用光学フィルター１０４ａを設ければ、各検出領域１１に、所望の波長の励起光を選択的に照射することができる。 (4) Optical filters 104a and 104b
FIG. 10 is a schematic conceptual view schematically showing the second embodiment of the light detection device 10 according to the present invention. In the present embodiment, an excitation optical filter 104 a is provided between each light irradiation means 101 and each detection region 11. In the light detection apparatus 10 according to the present invention, the excitation optical filter 104a is not essential, but if the excitation optical filter 104a is provided as in the present embodiment, each detection region 11 has a desired wavelength. Excitation light can be selectively irradiated.

また、本実施形態では、各検出領域１１と各光検出手段１０２との間に、受光用光学フィルター１０４ｂを設けている。本発明に係る光検出装置１０では、この受光用光学フィルター１０４ｂは必須のものではないが、本実施形態のように受光用光学フィルター１０４ｂを設ければ、各検出領域１１中に存在するサンプル中から発せられる蛍光Ｆから、所望の波長の光を選択的に受光することができる。   In the present embodiment, a light receiving optical filter 104 b is provided between each detection region 11 and each light detection means 102. In the light detection device 10 according to the present invention, the light receiving optical filter 104b is not essential, but if the light receiving optical filter 104b is provided as in the present embodiment, the light receiving optical filter 104b is included in the sample existing in each detection region 11. It is possible to selectively receive light of a desired wavelength from the fluorescence F emitted from.

（５）アパーチャー１０５ａ、１０５ｂ、隔壁
図１１は、本発明に係る光検出装置１０の第３実施形態を模式的に示す模式概念図である。本実施形態では、各光照射手段１０１と各検出領域１１との間に、アパーチャー１０５ａを設けている。本発明に係る光検出装置１０では、このアパーチャー１０５ａは必須のものではないが、本実施形態のようにアパーチャー１０５ａを設ければ、各光照射手段１０１から、対応する検出領域１１以外の検出領域１１（隣の検出領域など）への光照射を防止することができ、その結果、ＳＮ比の向上を図ることが可能である。 (5) Apertures 105a and 105b, partition walls FIG. 11 is a schematic conceptual view schematically showing a third embodiment of the photodetector 10 according to the present invention. In the present embodiment, an aperture 105 a is provided between each light irradiation means 101 and each detection region 11. In the light detection apparatus 10 according to the present invention, the aperture 105a is not essential. However, if the aperture 105a is provided as in the present embodiment, the detection regions other than the corresponding detection region 11 are provided from each light irradiation means 101. 11 (adjacent detection region or the like) can be prevented from being irradiated with light, and as a result, the SN ratio can be improved.

また、本実施形態では、各検出領域１１と各光検出手段１０２との間にも、アパーチャー１０５ｂを設けている。本発明に係る光検出装置１０では、このアパーチャー１０５ｂも必須のものではないが、本実施形態のようにアパーチャー１０５ｂを設ければ、対応する検出領域１１以外の検出領域１１（隣の検出領域など）からのクロストークを低減することができ、その結果、ＳＮ比の向上を図ることが可能である。   In this embodiment, an aperture 105 b is also provided between each detection region 11 and each light detection means 102. In the light detection device 10 according to the present invention, the aperture 105b is not essential, but if the aperture 105b is provided as in the present embodiment, the detection region 11 other than the corresponding detection region 11 (adjacent detection region, etc.) ) Can be reduced, and as a result, the SN ratio can be improved.

なお、本発明に係る光検出装置１０では、アパーチャー１０５ａ、１０５ｂ以外に、図示しないが、レンズ間に隔壁を設けることでも、同様の効果を発揮することができる。   In the light detection device 10 according to the present invention, although not shown in addition to the apertures 105a and 105b, the same effect can be achieved by providing a partition wall between the lenses.

以上説明した本発明に係る光検出用チップ１やこれを用いた光検出用装置１０は、検出領域１１内のサンプル中に含まれる物質の物性に関する分析・解析を行うだけでなく、例えば、流路Ｃ内に検出領域１１を設け、電気泳動法と組み合わせることにより、サンプル中に含まれる物質の定量的な分析を行うことも可能である。   The light detection chip 1 according to the present invention described above and the light detection device 10 using the chip 1 not only perform analysis / analysis on the physical properties of the substances contained in the sample in the detection region 11 but also, for example, flow By providing the detection region 11 in the path C and combining with the electrophoresis method, it is possible to quantitatively analyze the substance contained in the sample.

また、例えば、液状サンプルをシース流で挟み込んでフローセルを形成し、そのフローセル中を流れる物質からの蛍光強度ないし蛍光画像を、光検出手段１０２で取得することもできる。このようなフローセルの構造としては、フローサイトメトリー技術として広く研究開発および実用化がなされているものを用いることができる。このように、マイクロ流路Ｃ内を通流中のサンプルから光検出を行うことで、流路下流において、サンプル中の細胞や核酸などの微小粒子を、得られた情報に基づいて分取することも可能である。   Alternatively, for example, a flow sample can be formed by sandwiching a liquid sample in a sheath flow, and fluorescence intensity or a fluorescence image from a substance flowing in the flow cell can be acquired by the light detection means 102. As the structure of such a flow cell, one that has been widely researched and developed and put into practical use as a flow cytometry technique can be used. In this way, by detecting light from the sample flowing through the microchannel C, the microparticles such as cells and nucleic acids in the sample are sorted based on the obtained information downstream of the channel. It is also possible.

本発明によれば、微量なサンプルから効率的に光を検出することが可能であり、分析精度および解析精度を向上させることが実現できる。   According to the present invention, light can be efficiently detected from a very small amount of sample, and analysis accuracy and analysis accuracy can be improved.

この技術を用いることで、医療分野（病理学、腫瘍免疫学、移植学、遺伝学、再生医学、化学療法など）、創薬分野、臨床検査分野、食品分野、農業分野、工学分野、法医学分野、犯罪鑑識分野、など様々な分野における分析・解析技術の向上に貢献することができる。   By using this technology, medical field (pathology, tumor immunology, transplantation, genetics, regenerative medicine, chemotherapy, etc.), drug discovery field, clinical laboratory field, food field, agricultural field, engineering field, forensic field , Can contribute to the improvement of analysis and analysis technology in various fields such as criminal forensics.

１ 光検出用チップ
１１ 検出領域
１２ 光反射手段
１３ 光透過制御手段
１０ 光検出装置
１０１ 光照射手段
１０２ 光検出手段
１０３ａ、１０３ｂ 集光レンズ
１０４ａ、１０４ｂ 光学フィルター
１０５ａ、１０５ｂ アパーチャー
Ｅ 励起光
Ｆ 蛍光
Ｄ 蛍光検出方向
Ｗ ウエル
Ｃ 流路
Ｔ 基板 DESCRIPTION OF SYMBOLS 1 Light detection chip | tip 11 Detection area | region 12 Light reflection means 13 Light transmission control means 10 Light detection apparatus 101 Light irradiation means 102 Light detection means 103a, 103b Condensing lenses 104a, 104b Optical filters 105a, 105b Aperture E Excitation light F Fluorescence D Fluorescence detection direction W Well C Channel T Substrate

Claims (16)

サンプルへの光照射によってサンプル中から発せられる蛍光の検出が行われる複数の検出領域と、
前記検出領域を介して前記蛍光を検出する方向と対向する位置に配置された光反射手段と、
が少なくとも備えられた光検出用チップ。 A plurality of detection regions in which fluorescence emitted from the sample is detected by light irradiation to the sample; and
A light reflecting means disposed at a position facing the direction of detecting the fluorescence via the detection region;
A chip for light detection provided with at least. 前記光反射手段が、複数の前記検出領域に対して複数配置された請求項１記載の光検出用チップ。   The light detection chip according to claim 1, wherein a plurality of the light reflecting means are arranged for a plurality of the detection regions. 前記光反射手段は、凹面形状を有する請求項１または２に記載の光検出用チップ。   The light detection chip according to claim 1, wherein the light reflecting means has a concave shape. 前記光反射手段は、平面形状を有する請求項１または２に記載の光検出用チップ。   The light detection chip according to claim 1, wherein the light reflecting means has a planar shape. 前記光反射手段は、サンプルへ照射される光を透過し、
サンプル中から発せられる蛍光を反射する請求項１から４のいずれか一項に記載の光検出用チップ。 The light reflecting means transmits light applied to the sample,
The light detection chip according to any one of claims 1 to 4, which reflects fluorescence emitted from a sample. サンプルへ照射される光を透過せず、
サンプル中から発せられる蛍光を透過する光透過制御手段が更に備えられた請求項１から５のいずれか一項に記載の光検出用チップ。 Does not transmit the light irradiated to the sample,
The light detection chip according to any one of claims 1 to 5, further comprising a light transmission control means for transmitting fluorescence emitted from the sample. サンプルへの光照射を行う光照射手段と、
該光照射手段からの光照射によってサンプル中から発せられる蛍光の検出が行われる複数の検出領域と、
前記蛍光を検出する光検出手段と、
前記検出領域を介して前記光検出手段と対向する位置に配置された光反射手段と、
が少なくとも備えられた光検出装置。 A light irradiation means for irradiating the sample with light;
A plurality of detection regions in which fluorescence emitted from the sample by light irradiation from the light irradiation means is detected;
A light detection means for detecting the fluorescence;
A light reflecting means disposed at a position facing the light detecting means via the detection region;
A photodetection device provided with at least. 前記光照射手段と前記検出領域の間には、集光レンズが備えられた請求項７記載の光検出装置。   The light detection device according to claim 7, wherein a condensing lens is provided between the light irradiation unit and the detection region. 前記光照射手段が、複数の前記検出領域に対して複数配置された請求項７または８に記載の光検出装置。   The light detection device according to claim 7 or 8, wherein a plurality of the light irradiation means are arranged for a plurality of the detection regions. 前記光反射手段が、複数の前記検出領域に対して複数配置された請求項７から９のいずれか一項に記載の光検出装置。   The light detection device according to any one of claims 7 to 9, wherein a plurality of the light reflecting means are arranged for a plurality of the detection regions. 前記光検出手段が、複数の前記検出領域に対して複数配置された請求項７から１０のいずれか一項に記載の光検出装置。   The photodetection device according to any one of claims 7 to 10, wherein a plurality of the light detection means are arranged for a plurality of the detection regions. 前記集光レンズが、複数の前記検出領域に対して複数配置された請求項８から１１のいずれか一項に記載の光検出装置。   The light detection apparatus according to claim 8, wherein a plurality of the condensing lenses are arranged for a plurality of the detection regions. 前記光反射手段は、凹面形状を有する請求項７から１２のいずれか一項に記載の光検出装置。   The light detection device according to claim 7, wherein the light reflecting means has a concave shape. 前記光反射手段は、平面形状を有する請求項７から１２のいずれか一項に記載の光検出装置。   The light detection device according to claim 7, wherein the light reflecting means has a planar shape. 前記光反射手段は、サンプルへ照射される光を透過し、
サンプル中から発せられる蛍光を反射する請求項７から１４のいずれか一項に記載の光検出装置。 The light reflecting means transmits light applied to the sample,
The photodetection device according to any one of claims 7 to 14, which reflects fluorescence emitted from the sample. サンプルへ照射される光を透過せず、
サンプル中から発せられる蛍光を透過する光透過制御手段が更に備えられた請求項７から１５のいずれか一項に記載の光検出装置。 Does not transmit the light irradiated to the sample,
The light detection device according to any one of claims 7 to 15, further comprising a light transmission control unit that transmits fluorescence emitted from the sample.
JP2009187313A 2009-08-12 2009-08-12 Light detection chip, and light detection device using the same Pending JP2011038922A (en)

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US20110037077A1 (en) 2011-02-17

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