JP2010523104A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010523104A5 JP2010523104A5 JP2010501666A JP2010501666A JP2010523104A5 JP 2010523104 A5 JP2010523104 A5 JP 2010523104A5 JP 2010501666 A JP2010501666 A JP 2010501666A JP 2010501666 A JP2010501666 A JP 2010501666A JP 2010523104 A5 JP2010523104 A5 JP 2010523104A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- cancer
- electrical signal
- cells
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 201000011510 cancer Diseases 0.000 claims 10
- 102000004190 Enzymes Human genes 0.000 claims 7
- 108090000790 Enzymes Proteins 0.000 claims 7
- 239000000758 substrate Substances 0.000 claims 6
- 230000003211 malignant Effects 0.000 claims 3
- 239000002246 antineoplastic agent Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- 206010009944 Colon cancer Diseases 0.000 claims 1
- 238000001574 biopsy Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 229940079593 drugs Drugs 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
Claims (14)
- ガン細胞を検出する方法であって、
（ａ）電気的シグナルをもたらすことができる生成物を生じさせるように、酵素が細胞と基質との反応を触媒する条件のもと、細胞を酵素のための基質と接触させること；および
（ｂ）前記電気的シグナルのレベルを測定すること
を含み、所定の閾値と比較した前記電気的シグナルのレベルの違いにより、ガン細胞が示される方法。 - 対象をガンと診断する方法であって、
（ａ）電気的シグナルをもたらすことができる生成物を生じさせるように、酵素が細胞と基質との反応を触媒する条件のもと、対象のサンプルにおける少なくとも１つの細胞を酵素のための基質と接触させること；および
（ｂ）前記電気的シグナルのレベルを測定すること
を含み、所定の閾値と比較した前記電気的シグナルのレベルの違いにより、ガンが示される方法。 - ガンのための処置を個々に最適化する方法であって、
（ａ）対象のサンプルにおける少なくとも１つのガン細胞を少なくとも１つの抗ガン剤と接触させること；
（ｂ）電気的シグナルをもたらすことができる生成物を生じさせるように、酵素が細胞と基質との反応を触媒する条件のもと、前記少なくとも１つのガン細胞を酵素のための基質と接触させること；および
（ｃ）細胞によって生じた前記電気的シグナルのレベルを測定すること
を含み、前記レベルにより、前記対象のガンを処置するための前記抗ガン剤の効力が示される方法。 - 細胞の悪性表現型を逆戻りさせることができる薬剤を同定する方法であって、
（ａ）少なくとも１つのガン細胞を薬剤に供すること；および
（ｂ）細胞の悪性表現型を、工程（ａ）の後、および、必要な場合には工程（ａ）に先だって、請求項１に記載の方法に従って測定すること
を含み、表現型の逆戻りにより、細胞の悪性表現型を逆戻りさせることができる薬剤が示される方法。 - 前記少なくとも１つの細胞は複数の細胞を含む、請求項２〜４のいずれかに記載の方法。
- 前記測定は、高速大量処理のための手段を使用して行われる、請求項１〜３のいずれかに記載の方法。
- 前記接触はインビトロまたはエクスビボで行われる、請求項１に記載の方法。
- 前記サンプルは、最大でも５００個の細胞を含む、請求項２〜３のいずれかに記載の方法。
- 前記サンプルは、最小でも１０個の細胞を含む、請求項２〜３のいずれかに記載の方法。
- 前記酵素はアルカリホスファターゼである、請求項１〜４のいずれかに記載の方法。
- 前記ガン細胞は結腸ガン細胞である、請求項１〜４のいずれかに記載の方法。
- 前記細胞は無傷である、請求項１〜４のいずれかに記載の方法。
- 前記細胞は生検サンプルに含まれる、請求項１〜３のいずれかに記載の方法。
- 前記細胞は予め処理されていない、請求項１〜３および１３のいずれかに記載の方法。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US90744107P | 2007-04-02 | 2007-04-02 | |
| PCT/IL2008/000457 WO2008120216A1 (en) | 2007-04-02 | 2008-04-02 | Methods of detecting cancer cells and use of same for diagnosing and monitoring treatment of the disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010523104A JP2010523104A (ja) | 2010-07-15 |
| JP2010523104A5 true JP2010523104A5 (ja) | 2011-05-06 |
Family
ID=39530621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010501666A Pending JP2010523104A (ja) | 2007-04-02 | 2008-04-02 | ガン細胞検出方法ならびにガン疾患の診断およびガン疾患の処置のモニタリングのためのその使用 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8268577B2 (ja) |
| EP (1) | EP2142661B1 (ja) |
| JP (1) | JP2010523104A (ja) |
| KR (1) | KR20100015774A (ja) |
| CN (1) | CN101702920B (ja) |
| WO (1) | WO2008120216A1 (ja) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010523104A (ja) | 2007-04-02 | 2010-07-15 | ラモット・アット・テル・アビブ・ユニバーシテイ・リミテッド | ガン細胞検出方法ならびにガン疾患の診断およびガン疾患の処置のモニタリングのためのその使用 |
| US9707403B2 (en) | 2007-12-12 | 2017-07-18 | Neuro Code Tech Holdings, Llc | Rapid destruction of malignant tumors by excitotoxicity and osmotic-shock medical tactics |
| US8656930B2 (en) | 2008-01-10 | 2014-02-25 | Neuro-Code Tech Holdings, LLC | Method and system for processing cancer cell electrical signals for medical therapy |
| WO2009151783A1 (en) | 2008-04-17 | 2009-12-17 | The Neuro-Signaling Foundation | System and method to elicit apoptosis in malignant tumor cells for medical treatment |
| WO2013011513A1 (en) * | 2011-07-21 | 2013-01-24 | Ramot at Tel- Aviv University Ltd. | Methods of determinig enzyme activity in preserved cell samples |
| RU2489710C1 (ru) * | 2012-02-20 | 2013-08-10 | Владимир Вадимович Мошкин | Способ коммутационной хроноамперометрии |
| EP2855697A1 (en) * | 2012-05-31 | 2015-04-08 | Ramot at Tel-Aviv University Ltd. | Methods and system for detecting melanoma |
| KR20150074487A (ko) | 2013-12-24 | 2015-07-02 | 삼성전자주식회사 | 식각 부산물 검출 방법 및 이를 이용한 자기 저항 메모리 장치의 제조 방법 |
| JP2015144904A (ja) * | 2015-04-20 | 2015-08-13 | 佐藤 洋 | 位置制御システム |
| US11154238B2 (en) | 2015-08-07 | 2021-10-26 | Electroceuticals, Llc | Systems, methods and apparatuses for providing bioelectronic neurocode-based therapies to mammals |
| CN113720893A (zh) * | 2021-09-01 | 2021-11-30 | 致慧医疗科技(上海)有限公司 | 一种基于癌细胞表面电荷强度的评估肿瘤恶性程度的方法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154600B (nl) | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| NL154599B (nl) | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| US3901654A (en) | 1971-06-21 | 1975-08-26 | Biological Developments | Receptor assays of biologically active compounds employing biologically specific receptors |
| US3853987A (en) | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
| US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
| NL171930C (nl) | 1972-05-11 | 1983-06-01 | Akzo Nv | Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen. |
| US3850578A (en) | 1973-03-12 | 1974-11-26 | H Mcconnell | Process for assaying for biologically active molecules |
| US3935074A (en) | 1973-12-17 | 1976-01-27 | Syva Company | Antibody steric hindrance immunoassay with two antibodies |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4034074A (en) | 1974-09-19 | 1977-07-05 | The Board Of Trustees Of Leland Stanford Junior University | Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) |
| US3984533A (en) | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
| US4098876A (en) | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
| US4879219A (en) | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
| US5011771A (en) | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
| EP0523148A1 (en) * | 1990-04-03 | 1993-01-20 | Cellstat Technologies, Inc. | An electronic technique of identifying an effective drug for treating a cancer patient |
| US5281521A (en) | 1992-07-20 | 1994-01-25 | The Trustees Of The University Of Pennsylvania | Modified avidin-biotin technique |
| US6682648B1 (en) * | 1997-08-12 | 2004-01-27 | University Of Southern California | Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures |
| CN1312480C (zh) * | 2004-09-24 | 2007-04-25 | 南京大学 | 肿瘤细胞表面抗原的原位电化学免疫测定方法 |
| US20060205090A1 (en) * | 2005-03-14 | 2006-09-14 | Newton Michael W | Water-soluble conjugates for electrochemical detection |
| JP2010523104A (ja) * | 2007-04-02 | 2010-07-15 | ラモット・アット・テル・アビブ・ユニバーシテイ・リミテッド | ガン細胞検出方法ならびにガン疾患の診断およびガン疾患の処置のモニタリングのためのその使用 |
| KR101281761B1 (ko) * | 2009-03-27 | 2013-07-26 | 서강대학교산학협력단 | 전기검출기반 줄기세포 분화 측정용 센서 |
-
2008
- 2008-04-02 JP JP2010501666A patent/JP2010523104A/ja active Pending
- 2008-04-02 EP EP08720067A patent/EP2142661B1/en not_active Not-in-force
- 2008-04-02 KR KR1020097021991A patent/KR20100015774A/ko not_active Application Discontinuation
- 2008-04-02 WO PCT/IL2008/000457 patent/WO2008120216A1/en active Application Filing
- 2008-04-02 CN CN2008800182996A patent/CN101702920B/zh not_active Expired - Fee Related
-
2009
- 2009-03-05 US US12/379,974 patent/US8268577B2/en not_active Expired - Fee Related
-
2012
- 2012-07-18 US US13/551,642 patent/US8530179B2/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010523104A5 (ja) | ||
| Wang et al. | Atherosclerosis-related circulating miRNAs as novel and sensitive predictors for acute myocardial infarction | |
| Bellassai et al. | Surface plasmon resonance for biomarker detection: advances in non-invasive cancer diagnosis | |
| Ou et al. | Cellular senescence in cancer: from mechanisms to detection | |
| Miao et al. | Gold nanoparticles-based multipedal DNA walker for ratiometric detection of circulating tumor cell | |
| Cooks et al. | Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 | |
| Fan et al. | High-sensitive and multiplex biosensing assay of NSCLC-derived exosomes via different recognition sites based on SPRi array | |
| Weng et al. | Highly sensitive and reliable detection of microRNA for clinically disease surveillance using SERS biosensor integrated with catalytic hairpin assembly amplification technology | |
| Zhou et al. | Urinary microRNA-30a-5p is a potential biomarker for ovarian serous adenocarcinoma | |
| Zampetaki et al. | Plasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes | |
| Mishra et al. | Recent advances in salivary cancer diagnostics enabled by biosensors and bioelectronics | |
| JP5291241B2 (ja) | 診断マーカーとしてのエキソソーム関連マイクロｒｎａ | |
| Al-Khanbashi et al. | Tissue and serum miRNA profile in locally advanced breast cancer (LABC) in response to neo-adjuvant chemotherapy (NAC) treatment | |
| Romiti et al. | Circulating tumor cells count predicts survival in colorectal cancer patients | |
| JP2012500389A5 (ja) | ||
| Guo et al. | Exosomes and pancreatic diseases: status, challenges, and hopes | |
| WO2007087612A3 (en) | Detection and diagnosis of smoking related cancers | |
| Shen et al. | A three-gene signature as potential predictive biomarker for irinotecan sensitivity in gastric cancer | |
| CN111356774A (zh) | 用于检测衰老细胞的新型生物标记物 | |
| Ohuchida et al. | Sonic hedgehog is an early developmental marker of intraductal papillary mucinous neoplasms: clinical implications of mRNA levels in pancreatic juice | |
| TW200643176A (en) | ZNFN3A1/breast cancer related gene ZNFN3A1 | |
| Liyanage et al. | Plasmonic-based biosensor for the early diagnosis of prostate cancer | |
| Wu et al. | Overexpressions of miR-212 are associated with poor prognosis of patients with pancreatic ductal adenocarcinoma | |
| Xie et al. | Clinical significance of circulating tumor cells and their expression of cyclooxygenase-2 in patients with nasopharyngeal carcinoma. | |
| Hu et al. | Both FOXP1 and p65 expression are adverse risk factors in diffuse large B-cell lymphoma: a retrospective study in China |