JP2009292741A - Soil-borne disease control microorganism material and method for soil-borne disease control - Google Patents

Soil-borne disease control microorganism material and method for soil-borne disease control Download PDF

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JP2009292741A
JP2009292741A JP2008145357A JP2008145357A JP2009292741A JP 2009292741 A JP2009292741 A JP 2009292741A JP 2008145357 A JP2008145357 A JP 2008145357A JP 2008145357 A JP2008145357 A JP 2008145357A JP 2009292741 A JP2009292741 A JP 2009292741A
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soil
disease control
material according
soil disease
controlling
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Yumi Magae
由美 馬替
Satoko Kanematsu
聡子 兼松
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Forestry and Forest Products Research Institute
National Agriculture and Food Research Organization
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Forestry and Forest Products Research Institute
National Agriculture and Food Research Organization
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a disease control microorganism material which is related to a new effective antagonizing microorganism, in stead of conventional synthetic agrochemicals, can easily, safely and effectively control Rosellinia necatrix in a real field, and realizes a soil-borne disease control technique capable of also contributing to environmental protection at low cost. <P>SOLUTION: Provided is a soil-borne disease control microorganism material containing the live mycelia of Flammulina velutipes as an active ingredient. The soil-borne disease control microorganism material selectively adopting the Flammulina velutipes as the microorganism antagonistically acting on pathogenic microorganisms can easily safely and effectively control the soil-borne diseases such as Rosellinia necatrix at low cost in real fields, and is never liable to give unnecessary loads to environments. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

本願発明は、土壌病害防除微生物資材ならびに土壌病害防除方法に関し、詳しくは、植物における白紋羽病菌に由来する病害の防除材ならびに防除方法に関するものである。 The present invention relates to a soil disease control microorganism material and a soil disease control method, and more particularly to a disease control material and a control method for diseases derived from white crested fungus in plants.

白紋羽病は、子のう菌の一種である白紋羽病菌(Rosellinia
necatrix)による重要な土壌病害であり、リンゴ、ナシ、ブドウ、及びウメなど果樹並びに材木等の永年作物、更にはダイズ及びスイセン等の草本植物を含む81科306種以上の植物を侵す多犯性の菌として知られている。
White crest feather disease is a kind of baby mold fungus (Rosellinia)
Necatrix) is an important soil disease that affects more than 306 species of plants in 81 families, including perennial crops such as fruit trees such as apples, pears, grapes, and ume and timber, as well as herbaceous plants such as soybeans and narcissus Known as a fungus.

白紋羽病の罹患植物は他の土壌病害と同様に、生育不良、衰弱、萎凋、黄化、根腐れ、及び早期落葉などが起こり枯死する。罹病植物体の地際部には特異的な白色-灰褐色の扇状の白色菌糸膜、菌糸束などが認められる。本病による永年作物の被害は大きく、土壌中及び罹病根上では擬似細核で生存し、その生存力は強く、防除の困難な土壌病害とされている。 Like other soil diseases, plants suffering from white crested feathers suffer from poor growth, weakness, wilt, yellowing, root rot, premature defoliation, etc. and die. Specific white-grey-brown fan-shaped white mycelium, mycelial bundles, etc. are observed on the border of affected plants. The damage of perennial crops due to this disease is large, and it survives in pseudofine nuclei in the soil and on the affected roots, and its viability is strong, and it is regarded as a soil disease that is difficult to control.

現在のところ、白紋羽病に対しての化学合成物質による農薬としては、クロルピクリン剤、イソプロチオラン剤、チオファネートメチル剤、ベノミル剤及びフルアジナム剤などが知られている。この中ではフルアジナム剤が多く使用されている。 At present, chlorpicrin agents, isoprothiolane agents, thiophanate methyl agents, benomyl agents, fluazinam agents, and the like are known as pesticides using chemically synthesized substances against white coat disease. Of these, fluazinam is often used.

しかし、フロンサイド(商標登録)に代表されるフルアジナム剤は、1樹あたりに必要な施肥量が多く(50〜200L)、また、隔年で土壌中に注入する必要があり、要する労力、コストが過大なものとなっている。 さら、フルアジナム剤は、人によっては皮膚接触によりかぶれを引き起こすうえ、魚毒性を持っており、環境に対する配慮、環境に負担を掛けない持続可能な農業への転換が求められている今日的課題のうえからも、化学薬剤に依存しない白紋羽病対策が必要となっている。   However, the fluazinam agent represented by Fronside (trademark registration) requires a large amount of fertilizer per tree (50 to 200 L), and it is necessary to inject it into the soil every other year, which requires labor and cost. It has become excessive. In addition, fluazinam drugs cause rashes due to skin contact in some people, and are also toxic to fish. Therefore, there is a need for environmental considerations and a shift to sustainable agriculture that does not place a burden on the environment. From the top, countermeasures against white coat rot that do not depend on chemical agents are necessary.

このため、これまでの化学合成農薬に代えて、生物防除を目的とした白紋羽病菌の拮抗微生物、例えばトリコデルマ属菌、Pseudomonas菌、放線菌などを用いての防除方法が開示されている。前記において例えば、土壌微生物の一種であるトリコデルマ属菌は、それ自体は、植物の各種病原菌に対して強い拮抗作用を有していることは従来から知られており、これを主成分とする殺菌剤が農薬登録されてもいる。   For this reason, a control method using an antagonistic microorganism of white rot fungus for the purpose of biological control, such as Trichoderma spp., Pseudomonas fungus, actinomycetes, etc., for biocontrol has been disclosed instead of the conventional chemically synthesized pesticides. In the above, for example, Trichoderma spp. Which is a kind of soil microorganism has been known to have a strong antagonistic action against various pathogens of plants per se, and sterilization based on this. The agent is also registered as an agrochemical.

しかしながら、トリコデルマ菌製剤については実用に供されているものが少ないのが現状である。 すなわち、トリコデルマ属菌、Pseudomonas菌などの拮抗作用に関しては、たとえ、実験室で効果が確認されても野外試験などでは生物防除活性が認められないことが多い。このことは、トリコデルマ属菌のように効果のある拮抗微生物を農場に投入しても、土壌中の各種微生物が競合している中では生存競争が激しく、期待した発病抑制効果が得られない可能性がある。 However, there are few Trichoderma bacteria preparations in practical use. That is, regarding the antagonistic action of Trichoderma spp., Pseudomonas fungus, etc., even if the effect is confirmed in the laboratory, the biocontrol activity is often not observed in the field test. This means that even if antagonistic microorganisms that are effective like Trichoderma spp. Are put on the farm, the competition for survival is intense while the various microorganisms in the soil are competing, and the expected disease control effect may not be obtained. There is sex.

さらに、トリコデルマ属菌はきのこの病害菌になり得る可能性を秘めており、これが生物農薬として広範囲に使用される環境はきのこ生産に新たな脅威を招来することになる
したがって、果物ときのことが同時に重要な生産物である地域にトリコデルマ属菌製剤を安易に使用することはできない。
In addition, Trichoderma spp. Has the potential to become mushroom pests, and the environment in which it is widely used as a biopesticide will pose a new threat to mushroom production. At the same time, Trichoderma spp. Cannot be easily used in areas that are important products.

また、放線菌の使用は、白紋羽病 の防除方法としては有効であるが、放線菌の菌体培養液を土壌に対し、20〜30%も添加しなければならず、放線菌の培養に多大の労力を必要とする。 The use of actinomycetes is effective as a method for controlling white coat rot, but 20 to 30% of the actinomycete cell culture solution must be added to the soil. Requires a lot of effort.

上記のほか、微生物資材による白紋羽病の防除としては、菌類ウイルスによる方法がある。 すなわち、菌類ウイルスによって病原菌の病原力を低下させるもので、具体的には特定の菌類ウイルスを白紋羽病菌に感染させる手法である。 In addition to the above, there is a method using fungal virus as a method for controlling white feather disease with microbial materials. In other words, it is a technique for reducing the virulence of pathogenic bacteria by fungal viruses, specifically, a technique for infecting a specific fungal virus with white coat fungus.

しかしながら、前記手法は白紋羽病菌の不和合性によってウイルスの伝播が阻害されるため実用化された常法となすためには克服すべき課題が残されている。 However, the above-mentioned method has a problem to be overcome in order to obtain a practical method that has been put to practical use because virus transmission is inhibited by the incompatibility of white rot fungus.

本願発明に関して、以下のような先行文献が存在している。
特開平06−256125号公報 特開平10−36211号公報 特開2002−338421号公報 特開2006−199601号公報 特許第3594905号公報 特許第3692395号公報 九州森林研究57:284−285,2004「エノキタケ栽培施設で発生したトリコデルマ被害について」 「根圏環境の動態解明と制御技術の開発」農林技術会議事務局研究成果274号、1992年、
Regarding the present invention, the following prior documents exist.
Japanese Patent Laid-Open No. 06-256125 JP-A-10-36211 JP 2002-338421 A JP 2006-199601 A Japanese Patent No. 3594905 Japanese Patent No. 3692395 Kyushu Forestry Research 57: 284-285, 2004 “About Trichoderma Damage Occurred at Enokitake Cultivation Facility” "Elucidation of rhizosphere environment dynamics and development of control technology," Agricultural and Forestry Technology Council Secretariat Research Result 274, 1992,

本願発明は、これまでの合成農薬に代えて、新規有効な拮抗微生物に係る土壌病菌防除微生物資材を提供し、白紋羽病防除を現実の圃場で容易、安全かつ効果的に為し得て、併せて環境保全にも資することのできる病害防除技術を低廉なコスト下に実現して、上記従来の課題を解決しようとするものである。 The present invention provides a microbial material for controlling soil diseases related to novel effective antagonistic microorganisms instead of the conventional synthetic pesticides, and can easily and safely and effectively control white coat rot in an actual field. In addition, the present invention aims to solve the above-mentioned conventional problems by realizing a disease control technique that can contribute to environmental conservation at a low cost.

本願発明は、エノキタケの生菌糸を有効成分とする土壌病害防除微生物資材を提供して、上記従来の課題を解決しようとするものである。   The present invention is to provide a soil disease controlling microbial material containing a raw hyphae of enokitake mushroom as an active ingredient, and to solve the above conventional problems.

また、上記の土壌病害防除微生物資材において、土壌病害に係る病原菌は子嚢菌類に属する土壌病原菌となすことがある。 In the above-mentioned soil disease control microorganism material, the pathogen associated with the soil disease may be a soil pathogen belonging to Ascomycota.

さらに、前記段落0016記載の土壌病害防除微生物資材において、子嚢菌類に属する病原菌は白紋羽病菌(Rosellinia necatrix)を対象とすることがある。 Furthermore, in the soil disease control microorganism material described in paragraph 0016, the pathogenic fungus belonging to the ascomycete fungus may be targeted against white rot fungus (Rosellinia necatrix).

またさらに、上記いずれか記載の土壌病害防除微生物資材において、前記含有する有効成分はエノキタケ生菌糸の残存する培養処理物で構成することがある。 Furthermore, in any one of the above-mentioned soil disease control microorganism materials, the contained active ingredient may be composed of a culture treated product in which enokitake mushrooms remain.

さらに、上記段落0018記載の土壌病害防除微生物資材において、前記培養処理物は固体培養に係る担体で構成することがある。 Furthermore, in the soil disease controlling microbial material according to paragraph 0018, the culture treatment product may be constituted by a carrier for solid culture.

また、上記段落0018記載の土壌病害防除微生物資材において、前記培養処理物は液体培養における液体で構成することがある。 Moreover, in the soil disease control microorganism material according to paragraph 0018, the culture treatment product may be composed of a liquid in liquid culture.

本願発明はまた、対象植物の根周の土壌に段落0015ないし段落0020いずれか記載の土壌病害防除微生物資材を施用してなる土壌病害防除方法を提供して、上記従来の課題を解決しようとするものである。 The present invention also provides a soil disease control method comprising applying the soil disease control microorganism material according to any one of paragraphs 0015 to 0020 to the soil around the root of the target plant, and attempts to solve the conventional problems described above. Is.

また、本願発明は、対象植物の根周の土壌に段落0015ないし段落0020いずれか記載の土壌病害防除微生物資材を施用してなる白紋羽病防除方法を提供して、上記従来の課題を解決しようとするものである。 In addition, the present invention provides a method for controlling white coat rot by applying the soil disease controlling microbial material according to any one of paragraphs 0015 to 0020 to the soil around the root of the target plant, thereby solving the above-described conventional problems. It is something to try.

さらに、本願発明は、対象植物を栽培する土壌に段落0015ないし段落0020いずれか記載の土壌病害防除微生物資材を灌注してなる土壌病害防除方法を提供して、上記従来の課題を解決しようとするものである。 Furthermore, the present invention provides a soil disease control method obtained by irrigating the soil disease control microorganism material according to any one of paragraphs 0015 to 0020 to the soil in which the target plant is cultivated, and attempts to solve the above-described conventional problems. Is.

さらにまた、本願発明は、対象植物の根周の土壌に段落0015ないし段落0020いずれか記載の土壌病害防除微生物資材を灌注してなる白紋羽病防除方法を提供して、上記従来の課題を解決しようとするものである。 Furthermore, the present invention provides a method for controlling white coat rot by irrigating the soil disease control microorganism material according to any one of paragraphs 0015 to 0020 into the soil around the root of the target plant, and the above-mentioned conventional problems are solved. It is something to be solved.

以上のように、本願発明にあっては、病原菌に拮抗的に作用する微生物としてエノキタケを選択採用することにより、白紋羽病等の土壌病害防除を低廉なコスト下に現実の圃場で容易、安全かつ効果的に為し得る一方、環境に不必要な負荷を与える恐れが全くないという効果を奏する。   As described above, in the present invention, by selecting and adopting enokitake as a microorganism that acts antagonistically against pathogenic bacteria, it is easy to control soil diseases such as white leaf blight in real fields at low cost, While it can be done safely and effectively, it has the effect that there is no risk of unnecessary load on the environment.

本願発明は、エノキタケの研究において知見された微生物拮抗作用を要旨とするものである。 すなわち、エノキタケの生菌糸による抗白紋羽病菌活性に係るものであり、この活性は、病原菌とエノキタケ菌糸の接触において示されるものである。したがって、病原菌の防除には、エノキタケの生菌糸自体を病原菌に接触させる必要がある。 The gist of the present invention is the microbial antagonism found in the research of Enokitake. That is, it is related to the anti-white coat fungus activity by the enokitake mushroom hyphae, and this activity is shown in the contact between the pathogenic fungus and the enokitake mushroom hyphae. Therefore, in order to control pathogenic bacteria, it is necessary to contact the enokitake mushroom hypha itself with the pathogenic bacteria.

エノキタケの生菌糸に関しては、エノキタケの菌体自体を使用できるが、現況ではエノキタケ栽培に係る廃菌床の使用が望ましい。エノキタケの生産現場では、日常的に大量の廃菌床が発生している。これらの廃菌床は野外に放置して堆肥化して利用するほか処理方法が見出されておらず、単なる廃棄物として放置せざるを得ないのが現状である。 このような状況において、エノキタケの廃菌床をそのままエノキタケの生菌源として活用できる意義は非常に大きい。 Although the enokitake mushroom cell itself can be used for the enokitake mushroom, the use of the waste fungus bed for enokitake mushroom cultivation is desirable in the present situation. A large amount of waste bed is generated on a daily basis at the production site of Enokitake. These waste bacteria beds are left in the field and composted for use, and no treatment method has been found, and it is necessary to leave them as mere waste. In such a situation, it is very significant that the enokitake mushroom bed can be used as it is as a source of live enokitake.

なお、前記廃菌床のほかに、栽培キノコ用培地以外のなんらかの担体に固体培養した菌糸体をその担体ごと使用することができる。このような担体としては、植物・樹木由来のもの、例えばウッドチップ、稲わらや、段ボール、新聞紙などパルプ系もなどがある。 これら固体担体の施用態様としては、防除対象植物としての例えばリンゴ樹の周辺にその根を囲繞するように埋設することが望ましい。 In addition to the waste fungus bed, mycelium that is solid-cultured on some carrier other than the culture medium for cultivated mushrooms can be used together with the carrier. Examples of such a carrier include those derived from plants and trees, such as wood chips, rice straw, cardboard, and newspaper-based pulp. As an application mode of these solid carriers, it is desirable to embed the roots around, for example, apple trees as plants to be controlled.

なお、前記固体担体のほかに、エノキタケを液体培養し、その生菌糸を含む培養液を防除剤として使用することができる。この場合、防除対象植物としての例えばリンゴ樹の根の周辺に培養液あるいはその水溶液を灌注して生菌糸を接種する。また、リンゴの植栽にあたり、苗木の根部を前記培養液あるいはその水溶液に浸漬して農地に植えつけてもよい。
また、苗木の植栽にあたっては、前記のほか植栽用地にあらかじめ廃菌床その他の固体担体をすきこむか、前記培養液あるいはその水溶液を撒布後に苗木を植生させてもよい。
In addition to the solid carrier, enokitake mushroom can be cultured in liquid and a culture solution containing the live mycelia can be used as a control agent. In this case, for example, a culture solution or an aqueous solution thereof is irrigated around the root of an apple tree as a plant to be controlled and inoculated with live mycelia. In planting apples, roots of seedlings may be soaked in the culture solution or an aqueous solution thereof and planted in farmland.
In planting seedlings, in addition to the above, a waste microbial bed or other solid carrier may be preliminarily placed in the planting site, or seedlings may be vegetated after spreading the culture solution or an aqueous solution thereof.

以下、本願発明を実施例により具体的に説明する。エノキタケの抗白紋派病効果は実際に、リンゴ(フジ)の苗木を用いて野外ポット試験で確認した。 その結果、数ヶ月に及びエノキタケを添加したポットでは白紋羽病によるリンゴの枯死は見られなかった。 このことは、エノキタケが白紋羽病に対する防除機能を顕著に発揮し得ることを示している。以下、実施例(試験例)により具体的に説明する。   Hereinafter, the present invention will be described in detail by way of examples. The anti-white herring disease effect of Enokitake was actually confirmed by an outdoor pot test using an apple (Fuji) seedling. As a result, in the pot added with enokitake mushrooms for several months, apples were not killed by white spotted feathers. This indicates that enokitake mushrooms can remarkably exert a control function against white coat feather disease. Hereinafter, the embodiment (test example) will be specifically described.

比較データを要添付 リンゴ苗木ポット試験
(使用菌株:エノキタケ栽培品種A、 野生株B, 白紋羽病菌株W)
リンゴ(フジ)の苗木を用いて、ポット試験を行った。図1、図2は、ポットにおける苗木の植立構造を示す一部断面説明図である。 図1は、ポット(イ)を示しており、このポット(イ)は、図示のようにポット1の下部において、リンゴ(フジ)2の根部3の下半部は園芸用土4で覆い、上半部をエノキタケ(オガ粉・米ぬか培地ごと)5で断面がほぼ台形状となるように覆われている。そして、前記エノキタケ5の周囲は白紋羽病菌株W(オガ粉・米ぬか培地ごと)が埋め込まれ、その上部を赤玉土6で被覆してある。
Attach comparative data Apple seedling pot test (Strain used: Enokitake cultivar A, wild strain B, white coat rot strain W)
Pot tests were conducted using apple (Fuji) seedlings. 1 and 2 are partial cross-sectional explanatory views showing a planting structure of a seedling in a pot. FIG. 1 shows a pot (I). As shown in the figure, the pot (I) is covered with gardening soil 4 at the bottom of the pot 1 and the lower half of the root 3 of the apple (Fuji) 2 is covered with gardening soil 4. The half is covered with enokitake (with sawdust and rice bran medium) 5 so that the cross-section is substantially trapezoidal. The enokitake mushroom 5 is embedded with a white coat rot strain W (with sawdust and rice bran medium), and the upper part thereof is covered with red bean paste 6.

他方、図2はポット(ロ)を示しており、このポット(ロ)は、図示のようにポット1の下部において、リンゴ(フジ)2の根部3は全体を園芸用土4で覆われ、この園芸用土4の上部は、園芸用土4を囲繞するように白紋羽病菌株W(オガ粉・米ぬか培地ごと)が埋め込まれている。 On the other hand, FIG. 2 shows a pot (b). As shown in the figure, the pot (b) has a root 3 of an apple (fuji) 2 entirely covered with gardening soil 4 in the lower part of the pot 1. The upper part of the horticultural soil 4 is embedded with white coat rot strain W (both oga flour and rice bran medium) so as to surround the horticultural soil 4.

また、図3は、ポット(ハ)を示しており、このポット(ハ)はリンゴ(フジ)2の根部3の下半部は園芸用土4で覆われ、上半部はエノキタケ5(オガ粉・米ぬか培地ごと)で覆われている。   FIG. 3 shows a pot (c). The pot (c) is covered with gardening soil 4 in the lower half 3 of the root 3 of the apple (Fuji) 2 and enokitake mushroom 5 (soda flour).・ Covered with rice bran medium.

試験に使用したエノキタケは、前記のように2種で、すなわち栽培品種A,と野生株Bである。 したがって、ポット試験はエノキタケの2品種に応じて、以下の2種のグループを構成して行った。   There are two types of enokitake mushrooms used in the test, namely, cultivar A and wild strain B as described above. Therefore, the pot test was conducted in the following two groups according to the two varieties of enokitake.

グループ3−1
ポット(イ): エノキタケ5は栽培品種Aである。
ポット(ロ): エノキタケなし、白紋羽病菌株Wのみリンゴ苗木の周囲に施用。
ポット(ハ): エノキタケ5は栽培品種Aである。
Group 3-1
Pot (I): Enokitake 5 is cultivar A.
Pot (b): No enokitake mushroom, only white rot strain W is applied around apple seedlings.
Pot (c): Enokitake 5 is cultivar A.

グループ3−2
ポット(イ): エノキタケ5は野生品種Bである。
ポット(ロ): エノキタケなし、白紋羽病菌株Wのみリンゴ苗木の周囲に施用。
ポット(ハ): エノキタケ5は野生品種Bである。
Group 3-2
Pot (I): Enokitake 5 is a wild variety B.
Pot (b): No enokitake mushroom, only white rot strain W is applied around apple seedlings.
Pot (c): Enokitake 5 is a wild variety B.

生育の経過
a: 上記グループ3−1、3−2のいずれにおいても、試験開始5ヶ月経過の時点で、手続補足書により提出する資料写真1に示すようにポット(イ)およびポット(ハ)にあってリンゴ苗木に枯死はみられなかったが、ポット(ロ)では試験開始3ヶ月の時点でリンゴ苗木は完全に枯死した。
b: 各グループにおいて、ポット(ハ)に係るリンゴ苗木は上記いずれの時点でも順調な生育が見られた。 根の発達も非常によく、エノキタケの白色菌糸は認められなかった。
このことから、エノキタケをリンゴ苗木のまわりに接種しても、リンゴに害を及ぼさないことが明らかになった。
Growth process
a: In any of the above groups 3-1 and 3-2, when 5 months have passed since the start of the test, as shown in the material photo 1 submitted by the supplementary procedure, it is in pot (b) and pot (c). Although no apple seedlings were found dead, the apple seedlings were completely dead in the pot (b) at 3 months from the start of the test.
b: In each group, apple seedlings related to the pot (c) grew smoothly at any of the above points. The roots developed very well, and no white hyphae of enokitake were observed.
From this, it was clarified that enokitake mushrooms were inoculated around apple seedlings without harming apples.

当該試験において、上記のようにエノキタケを試用した(イ)にあっては、8ヵ月後に苗木が枯死したが、ポットの土中に白紋羽病菌の菌糸の生育は認められなかったことから、8ヶ月までの間隔をあけずに、年数回あたりエノキタケを土中に補填すれば白紋羽病の防除を継続できることが知見できる。   In the test, in the case of (i) using the enokitake mushroom as described above, the seedlings died after 8 months, but the growth of white mycosis fungi was not observed in the soil of the pot. It can be found that if enokitake is filled in the soil several times a year without spacing up to 8 months, the control of white rot can be continued.

また、新たにリンゴ苗木を移植する際、従来法で土壌消毒を行った後、エノキタケ菌糸を添加してから植樹すれば、白紋羽病の防除に効果的である。 この際、エノキタケ菌糸添加には、従来、処理に困っていたエノキタケ廃菌床を使用すればコストはほとんどかからない。 In addition, when a new apple seedling is transplanted, soil sterilization is performed by a conventional method, and then planting is performed after adding enokitake mycelium, which is effective for controlling white rot. At this time, the addition of enokitake mycelia costs little if the use of the enokitake mushroom waste fungus bed, which has conventionally been difficult to process.

ポット(イ)における苗木の植立構造を示す一部断面説明図である。It is a partial cross-section explanatory drawing which shows the planting structure of the seedling in a pot (I). ポット(ロ)における苗木の植立構造を示す一部断面説明図である。It is a partial cross-section explanatory drawing which shows the planting structure of the seedling in a pot (b). ポット(ハ)における苗木の植立構造を示す一部断面説明図である。It is a partial cross-section explanatory drawing which shows the planting structure of the seedling in a pot (c).

符号の説明Explanation of symbols

1.............ポット
2.............リンゴ苗木
3.............苗木根部
4.............園芸用土
5.............エノキタケ
W.............白紋羽病菌株
6.............赤玉土
1. . . . . . . . . . . . . Pot 2. . . . . . . . . . . . . 2. Apple seedlings . . . . . . . . . . . . Seedling root 4. . . . . . . . . . . . . 4. Horticulture soil . . . . . . . . . . . . Enokitake W. . . . . . . . . . . . . White coat disease 6 . . . . . . . . . . . . Red crust

Claims (10)

エノキタケの生菌糸を有効成分として含有する土壌病害防除微生物資材。 A soil disease control microbial material containing live mycelia of Enokitake as an active ingredient. 請求項1記載の土壌病害防除微生物資材において、土壌病害に係る病原菌が子嚢菌類に属する土壌病害菌防除材。 The soil disease-control microbial material according to claim 1, wherein the pathogen associated with the soil disease belongs to the ascomycetes. 請求項2記載の土壌病害菌防除微生物資材において、子嚢菌綱に属する土壌病菌は白紋羽病菌(Rosellinia necatrix)であることを特徴とする土壌病菌防除微生物資材。 3. The soil disease-controlling microorganism material according to claim 2, wherein the soil-borne fungus belonging to the Ascomycetes class is Rosellinia necatrix. 請求項1ないし3いずれか記載の土壌病害防除微生物資材において、前記含有する有効成分はエノキタケ生菌糸の残存する培養処理物で構成したことを特徴とする土壌病害防除微生物資材。 The soil disease controlling microbial material according to any one of claims 1 to 3, wherein the active ingredient is composed of a cultured product in which enokitake mushrooms remain. 請求項4記載の土壌病害防除微生物資材において、前記培養処理物は固体培養に係る担体であることを特徴とする土壌病害防除微生物資材。 The soil disease controlling microbial material according to claim 4, wherein the culture treatment product is a carrier for solid culture. 請求項4記載の土壌病害防除微生物資材において、前記培養処理物は液体培養における液体であることを特徴とする土壌病害防除微生物資材。 5. The soil disease controlling microorganism material according to claim 4, wherein the culture treatment product is a liquid in liquid culture. 対象植物の根周の土壌に請求項1ないし6いずれか記載の土壌病害防除微生物資材を施用してなる土壌病害防除方法。 A soil disease control method comprising applying the soil disease control microorganism material according to any one of claims 1 to 6 to the soil around the root of the target plant. 対象植物の根周の土壌に請求項1ないし6いずれか記載の土壌病害防除微生物資材を施用してなる白紋羽病防除方法。 A method for controlling white crest feathers, comprising applying the soil disease controlling microorganism material according to any one of claims 1 to 6 to the soil around the root of the target plant. 対象植物を栽培する土壌に請求項1ないし6いずれか記載の土壌病害菌防除微生物資材を灌注してなる土壌病害防除方法。 The soil disease control method formed by irrigating the soil disease-controlling microbe material in any one of Claims 1 thru | or 6 to the soil which grows a target plant. 対象植物の根周の土壌に請求項1ないし6いずれか記載の土壌病害防除微生物資材を灌注してなる白紋羽病防除方法。 A method for controlling white coat rot, which comprises irrigating the soil around the root of the target plant with the soil disease controlling microorganism material according to any one of claims 1 to 6.
JP2008145357A 2008-06-03 2008-06-03 Soil-borne disease control microorganism material and method for soil-borne disease control Withdrawn JP2009292741A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011140463A (en) * 2010-01-07 2011-07-21 Tottori Univ Technique for controlling plant disease using waste mushroom bed of edible mushroom
JP2018177799A (en) * 2017-04-19 2018-11-15 小山 修 Preventive method of soil disease injury of plant
US11000035B2 (en) 2015-01-26 2021-05-11 National Research And Development Agency National Agriculture And Food Research Organization Soil-borne disease control method, soil for plant cultivation use, and soil-borne disease control agent

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011140463A (en) * 2010-01-07 2011-07-21 Tottori Univ Technique for controlling plant disease using waste mushroom bed of edible mushroom
US11000035B2 (en) 2015-01-26 2021-05-11 National Research And Development Agency National Agriculture And Food Research Organization Soil-borne disease control method, soil for plant cultivation use, and soil-borne disease control agent
JP2018177799A (en) * 2017-04-19 2018-11-15 小山 修 Preventive method of soil disease injury of plant
JP7096696B2 (en) 2017-04-19 2022-07-06 修 小山 Plant soil disease control method

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