JP2009103668A - Analyzing vessel and analyzing apparatus - Google Patents

Analyzing vessel and analyzing apparatus Download PDF

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JP2009103668A
JP2009103668A JP2007278249A JP2007278249A JP2009103668A JP 2009103668 A JP2009103668 A JP 2009103668A JP 2007278249 A JP2007278249 A JP 2007278249A JP 2007278249 A JP2007278249 A JP 2007278249A JP 2009103668 A JP2009103668 A JP 2009103668A
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Prior art keywords
operation chamber
analysis container
liquid
analysis
liquid sample
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JP2007278249A
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JP5273986B2 (en
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Takashi Miki
崇 三木
Hiroshi Saeki
博司 佐伯
Takuya Suzuki
拓也 鈴木
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Panasonic Corp
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Panasonic Corp
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Priority to JP2007278249A priority Critical patent/JP5273986B2/en
Application filed by Panasonic Corp filed Critical Panasonic Corp
Priority to CN201310374415.0A priority patent/CN103424543B/en
Priority to EP16195057.1A priority patent/EP3141901B1/en
Priority to EP18188222.6A priority patent/EP3447494B1/en
Priority to PCT/JP2008/002779 priority patent/WO2009044552A1/en
Priority to CN2008801022246A priority patent/CN101796420B/en
Priority to CN201210356041.5A priority patent/CN102879558B/en
Priority to EP08836397.3A priority patent/EP2209008B1/en
Priority to US12/681,493 priority patent/US8415140B2/en
Publication of JP2009103668A publication Critical patent/JP2009103668A/en
Priority to US13/770,499 priority patent/US8956879B2/en
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Abstract

<P>PROBLEM TO BE SOLVED: To provide an analyzing vessel which enables mixing a minute amount of liquid with a reagent by sufficiently stirring them. <P>SOLUTION: The analyzing vessel has an operation chamber 5 to which a liquid specimen 9 flowing in for stirring the liquid specimen 9 in the operation chamber 5 by an acceleration during rocking action. The shape of the internal peripheral wall of the operation chamber 5 is characterized by being formed with tilting wall surfaces 5a, 5b convergent toward outer most position 5c from rotating interior side 11 while rotating action. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

本発明は、医療分野や環境分野において、試料液の分析に必要な分析容器と分析装置に関するものである。   The present invention relates to an analysis container and an analysis apparatus necessary for analyzing a sample solution in the medical field and the environment field.

従来、血液、血漿、尿など体液、あるいは汚水などの試料液中の成分を光学的に分析するために、特許文献１には、図５（ａ）（ｂ）に示すように分析容器を用いた分析装置がある。   Conventionally, in order to optically analyze components in a sample liquid such as blood, plasma, urine, or body fluid, or sewage, an analysis container is used in Patent Document 1 as shown in FIGS. There was an analyzer.

この分析容器は、上面および下面に透過面を有している容器本体２００と、試料が入った毛細管ホールダ２０１と、希釈液が入った希釈液容器２０２とで構成されている。なお、２０３は試薬である。   This analysis container is composed of a container body 200 having a transmission surface on the upper surface and the lower surface, a capillary holder 201 containing a sample, and a diluent container 202 containing a diluent. Reference numeral 203 denotes a reagent.

その分析工程は、試料をサンプリングした毛細管ホールダ２０１を図５（ａ）に示すように容器本体２００に差し込み、希釈液容器２０２から容器本体２００へ希釈液２０４を供給する。次に、容器本体２００を、毛細管ホールダ２０１の試料が希釈液２０４に浸かる図５（ａ）の状態から図５（ｂ）のように試料が溶け込んだ希釈液２０４に試薬２０３が浸る状態、図５（ｂ）の状態から図５（ａ）の状態になるように繰り返し揺動させて、試料が溶け込んだ希釈液に試薬を混ぜる。その後に容器本体２００の角部２０５に溜まった混合液に光学的にアクセスして、透過光から成分を読み取るように構成されている。
特開平３−４６５６６号（図１） In the analysis step, the capillary holder 201 obtained by sampling the sample is inserted into the container body 200 as shown in FIG. 5A and the diluent 204 is supplied from the diluent container 202 to the container body 200. Next, the state in which the reagent 203 is immersed in the diluent 204 in which the sample is dissolved as shown in FIG. 5B from the state of FIG. 5A in which the sample of the capillary holder 201 is immersed in the diluent 204 is shown in FIG. The reagent is mixed with the diluted solution in which the sample is dissolved by repeatedly rocking from the state of 5 (b) to the state of FIG. 5 (a). Thereafter, the liquid mixture accumulated in the corner portion 205 of the container body 200 is optically accessed to read the component from the transmitted light.
Japanese Patent Laid-Open No. 3-46566 (FIG. 1)

しかしながら前記従来の構成では、前記回転もしくは揺動によって重力を用いて試料と希釈液の混合液を移動させて試薬と混合しようとしているにもかかわらず、分析容器中の液量が微量（数十μｌ程度）であるため、分析容器の内壁の表面と前記混合液との間に生ずる表面張力の作用によって、混合液が分析容器の内壁に引き付けられて動きにくい状態になっており、攪拌が不十分であるのが現状である。   However, in the above-described conventional configuration, the amount of liquid in the analysis container is very small (several tens of minutes) although the mixture of the sample and the diluted solution is moved by gravity by the rotation or swinging and is mixed with the reagent. Therefore, the liquid mixture is attracted to the inner wall of the analysis container due to the action of the surface tension generated between the surface of the inner wall of the analysis container and the liquid mixture, and stirring is not possible. The current situation is sufficient.

本発明は、前記従来の課題を解決するもので、微量の液と試薬を十分に攪拌して混合できる分析容器及び攪拌装置を提供することを目的とする。   The present invention solves the above-described conventional problems, and an object thereof is to provide an analysis container and a stirring device that can sufficiently mix and mix a small amount of liquid and a reagent.

本発明の請求項１記載の分析容器は、液体試料が流れ込む操作チャンバーを有し、揺動動作中の加速度によって前記操作チャンバー内の前記液体試料を攪拌する分析容器であって、前記操作チャンバーの内周壁の形状を、前記揺動動作中の内周側から最外周位置に向かって先すぼまりの傾斜した壁面で形成したことを特徴とする。   An analysis container according to claim 1 of the present invention is an analysis container having an operation chamber into which a liquid sample flows, and stirring the liquid sample in the operation chamber by acceleration during a swinging operation. The shape of the inner peripheral wall is formed by a sloping wall surface tapered from the inner peripheral side during the swinging operation toward the outermost peripheral position.

本発明の請求項２記載の分析容器は、請求項１において、前記操作チャンバーの内周壁の前記最外周位置の形状が、円弧形状であることを特徴とする。
本発明の請求項３記載の分析容器は、請求項１において、前記操作チャンバー内の内周面が撥水処理されていることを特徴とする。 The analysis container according to claim 2 of the present invention is characterized in that, in claim 1, the shape of the outermost peripheral position of the inner peripheral wall of the operation chamber is an arc shape.
The analysis container according to claim 3 of the present invention is characterized in that, in claim 1, the inner peripheral surface in the operation chamber is subjected to water repellent treatment.

本発明の請求項４記載の分析容器は、請求項１において、前記操作チャンバー内に界面活性剤を含んだ試薬を設けたことを特徴とする。
本発明の請求項５記載の分析装置は、試料液が採取された請求項１記載の分析容器がセットされる分析装置であって、回転軸芯を持ち前記分析容器を保持する回転体と、前記分析容器に遠心力が作用するように前記回転体を回転させる回転駆動部と、前記分析容器の前記操作チャンバー内の液体にアクセスして測定する測定手段とを有することを特徴とする。 The analysis container according to claim 4 of the present invention is characterized in that, in claim 1, a reagent containing a surfactant is provided in the operation chamber.
The analysis apparatus according to claim 5 of the present invention is an analysis apparatus in which the analysis container according to claim 1 from which the sample liquid has been collected is set, and a rotating body having a rotation axis and holding the analysis container; A rotation drive unit that rotates the rotating body so that a centrifugal force acts on the analysis container, and a measurement unit that accesses and measures the liquid in the operation chamber of the analysis container.

本発明の請求項６記載の分析装置は、請求項５において、前記回転駆動部は、前記分析容器に保持された前記試料液に働く表面張力よりも大きい遠心力を発生させることを特徴とする。   The analyzer according to claim 6 of the present invention is characterized in that, in claim 5, the rotation driving unit generates a centrifugal force larger than a surface tension acting on the sample liquid held in the analysis container. .

本発明の分析容器及び攪拌装置によれば、微量な液体試料と試薬を混合攪拌でき、さらに分析容器を小型化することができる。   According to the analysis container and the stirring device of the present invention, a small amount of liquid sample and reagent can be mixed and stirred, and the analysis container can be further downsized.

以下に、本発明の分析容器と攪拌装置の実施の形態を図面とともに詳細に説明する。
図１は分析容器を示す。
分析容器１は、図１（ａ）に示すようにベース基板２とこのベース基板２の上面を閉塞するカバー基板３とで構成されている。ベース基板２には、液体収容チャンバー４と、操作チャンバー５と、液体収容チャンバー４と操作チャンバー５とを接続する連結流路６が形成されている。カバー基板３には空気の吸排を行う空気穴７ａ，７ｂが形成されている。 Embodiments of an analysis container and a stirring device of the present invention will be described below in detail with reference to the drawings.
FIG. 1 shows an analysis container.
The analysis container 1 is composed of a base substrate 2 and a cover substrate 3 that closes the upper surface of the base substrate 2 as shown in FIG. In the base substrate 2, a liquid storage chamber 4, an operation chamber 5, and a connection channel 6 that connects the liquid storage chamber 4 and the operation chamber 5 are formed. Air holes 7 a and 7 b for sucking and discharging air are formed in the cover substrate 3.

ベース基板２とこのカバー基板３との貼り合わせに際しては、操作チャンバー５に予め試薬８がセットされている。液体収容チャンバー４には液体試料９が注入されている。
この分析容器１は図２に示すように、回転体としての傾斜した回転テーブル１０の上に、液体収容チャンバー４が回転テーブル１０の回転軸心１１の側で、回転テーブル１０の外周側に操作チャンバー５が位置するようにセットされる。回転駆動部１２は、回転軸心１１を中心に回転テーブル１０を回転させて分析容器１に遠心力を作用させる。もしくは所定の角度で反復回転させて揺動動作を行う、例えばモータである。なお、図２ではカバー基板３の表記が省かれている。 When the base substrate 2 and the cover substrate 3 are bonded together, the reagent 8 is set in the operation chamber 5 in advance. A liquid sample 9 is injected into the liquid storage chamber 4.
As shown in FIG. 2, the analysis container 1 is operated on the rotary table 10 that is inclined as a rotary body, and the liquid storage chamber 4 is operated on the rotary axis 10 side of the rotary table 10 and on the outer peripheral side of the rotary table 10. It is set so that the chamber 5 is positioned. The rotation drive unit 12 rotates the rotary table 10 around the rotation axis 11 to apply a centrifugal force to the analysis container 1. Alternatively, for example, a motor that performs a rocking motion by repeatedly rotating at a predetermined angle. In FIG. 2, the notation of the cover substrate 3 is omitted.

空気穴７ａ，７ｂは液体収容チャンバー４から操作チャンバー５へ遠心力により液体試料６を移送できるように空気の取り込みまたは排出を行う。これは、液体試料９が移動するには移動先から移動元に空気が流れ込む必要があるが、連結流路６は液体試料９で満たされており、空気穴７ａがないと空気が移動できないためである。   The air holes 7 a and 7 b take in or discharge air so that the liquid sample 6 can be transferred from the liquid storage chamber 4 to the operation chamber 5 by centrifugal force. This is because the liquid sample 9 needs to flow from the destination to the source in order to move, but the connection channel 6 is filled with the liquid sample 9, and the air cannot move without the air hole 7a. It is.

また、液体試料９が空気穴７ａを通過して外に飛散することを防ぐために、空気穴７ａ，７ｂは操作チャンバー５よりも回転軸心１１寄りに配置されている。
前記操作チャンバー５の形状は、連結流路６とは反対側の奥側（後述の外周）の幅が次第に狭くなる形状で、回転テーブル１０の回転方向と交差する方向の壁面５ａ，５ｂが分析容器１の外周部分で交わって操作チャンバー５の先端部５ｃが構成されている。 Further, in order to prevent the liquid sample 9 from passing through the air holes 7 a and scattering outside, the air holes 7 a and 7 b are arranged closer to the rotation axis 11 than the operation chamber 5.
The shape of the operation chamber 5 is such that the width on the back side (the outer periphery to be described later) opposite to the connection flow path 6 is gradually narrowed, and the wall surfaces 5a and 5b in the direction intersecting the rotation direction of the turntable 10 are analyzed. A front end portion 5 c of the operation chamber 5 is formed by intersecting at the outer peripheral portion of the container 1.

分析装置に上記の分析容器１をセットした分析工程に従って構成を説明する。
初めに、液体試料９は液体収容チャンバー４に保持されている。回転駆動部１２が回転テーブル１０を回転させると遠心力が生じ、連結流路６によって液体試料９が液体収容チャンバー４から操作チャンバー５へ移送される。移送された液体試料９は操作チャンバー５の先端部５ｃに流入し試薬８を浸す。回転駆動部１２は揺動動作を行い、操作チャンバー５内で液体試料９と試薬８が混合される。 The configuration will be described according to the analysis process in which the analysis container 1 is set in the analyzer.
First, the liquid sample 9 is held in the liquid storage chamber 4. When the rotary drive unit 12 rotates the rotary table 10, a centrifugal force is generated, and the liquid sample 9 is transferred from the liquid storage chamber 4 to the operation chamber 5 through the connection channel 6. The transferred liquid sample 9 flows into the tip 5c of the operation chamber 5 and immerses the reagent 8. The rotation driving unit 12 performs a swinging operation, and the liquid sample 9 and the reagent 8 are mixed in the operation chamber 5.

ここで、揺動動作時に操作チャンバー５の混合液にかかる力を説明する。
図３はカバー基板３の表記を省いて揺動動作中の分析容器と操作チャンバー５に流入した液体試料９の液面１３を表示した平面図、図４は図３における操作チャンバー５の壁面５ａ，５ｂを示したもので、操作チャンバー５の液体試料９には壁面５ｂから次のような力が作用する。壁面５ａの場合も同様である。 Here, the force applied to the liquid mixture in the operation chamber 5 during the swinging operation will be described.
FIG. 3 is a plan view in which the cover substrate 3 is omitted and the analysis container during the swinging operation and the liquid surface 13 of the liquid sample 9 flowing into the operation chamber 5 are displayed, and FIG. 4 is a wall surface 5a of the operation chamber 5 in FIG. , 5b, and the following force acts on the liquid sample 9 in the operation chamber 5 from the wall surface 5b. The same applies to the wall surface 5a.

揺動動作時の壁面５ｂに接触している液体試料９には、回転の加減速時に起こる加速度Ａと遠心力Ｂがかかっている。この二つの力を斜面上の力に置き換えると、加速度の分力Ｃと遠心力の分力Ｄに置き換わる。さらに、液体試料９には表面張力Ｅが加わり、このときの液体試料９にかかる力は、加速度の分力Ｃと表面張力Ｅと遠心力の分力Ｄの和になる。   The liquid sample 9 that is in contact with the wall surface 5b during the swinging operation is subjected to acceleration A and centrifugal force B that occur during acceleration / deceleration of rotation. When these two forces are replaced with forces on the slope, they are replaced with a component force C of acceleration and a component force D of centrifugal force. Furthermore, a surface tension E is applied to the liquid sample 9, and the force applied to the liquid sample 9 at this time is the sum of the component force C of acceleration, the surface tension E, and the component force D of centrifugal force.

図４では表面張力Ｅと遠心力の分力Ｄの和よりも、加速度の分力Ｃの方が大きいため、液体試料９は加速度の分力Ｃの方向に動く。
したがって、傾斜した壁面５ａ，５ｂの条件としては、傾斜した壁面５ａ，５ｂの間（先端部５ｃ）に溜まった液体試料９の液面１３と傾斜した壁面５ａ，５ｂで形成される液面−壁面角θを、加速度の分力Ｃが遠心力の分力Ｄと表面張力Ｅの和より大きく、かつ内周方向を向くように設定することである。 In FIG. 4, since the acceleration component force C is larger than the sum of the surface tension E and the centrifugal force component D, the liquid sample 9 moves in the direction of the acceleration component force C.
Therefore, as the conditions of the inclined wall surfaces 5a and 5b, the liquid surface formed by the liquid surface 13 of the liquid sample 9 and the inclined wall surfaces 5a and 5b accumulated between the inclined wall surfaces 5a and 5b (tip portion 5c)- The wall surface angle θ is set such that the component force C of acceleration is larger than the sum of the component force D of centrifugal force and the surface tension E, and faces the inner circumferential direction.

具体的には、液面−壁面角θが９０°の場合、加速度の分力Ｃはゼロになり、遠心力の分力Ｄは遠心力Ｂと等しくなる。そして、液体試料９は外周方向に移動しようとするが、先端部５ｃに溜まっているため、液体試料９は動かない。また、液面−壁面角θが９０°より小さい場合、加速度の分力Ｃと遠心力の分力Ｄは外周方向を向く。そして、液体試料９は外周方向に移動しようとするが、先端部５ｃに溜まっているため、液体試料９は動かない。   Specifically, when the liquid surface-wall angle θ is 90 °, the acceleration component force C becomes zero and the centrifugal force component D becomes equal to the centrifugal force B. And although the liquid sample 9 tends to move to an outer peripheral direction, since it has accumulated in the front-end | tip part 5c, the liquid sample 9 does not move. When the liquid surface-wall angle θ is smaller than 90 °, the acceleration component force C and the centrifugal component force D are directed in the outer circumferential direction. And although the liquid sample 9 tends to move to an outer peripheral direction, since it has accumulated in the front-end | tip part 5c, the liquid sample 9 does not move.

これに対してこの実施の形態では、液面−壁面角θが９０°より大きく形成されているため、表面張力Ｅと遠心力の分力Ｄの和よりも加速度の分力Ｃの方が大きい場合に、液体試料９は加速度の分力Ｃの方向に動くことができる。なお、液面−壁面角θの角度が９０°より大きくなるほど、小さな加速度で攪拌できる。   On the other hand, in this embodiment, since the liquid surface-wall angle θ is formed to be larger than 90 °, the acceleration component force C is larger than the sum of the surface tension E and the centrifugal force component D. In some cases, the liquid sample 9 can move in the direction of the component force C of acceleration. In addition, it can stir with small acceleration, so that the angle of liquid surface-wall surface angle (theta) becomes larger than 90 degrees.

さらに、傾斜した壁面５ａ，５ｂの長さは液体試料９が揺動動作時に十分に動けるために、液面１３よりも内周方向に延びている必要がある。
このように本実施の形態においては、操作チャンバー５の外周方向に先端部５ｃが形成されるように所定の角度で広がる傾斜した壁面５ａ，５ｂを形成することにより、回転方向に揺動動作時に液体試料９が操作チャンバー５内で十分に動くことができ、かつ少ない液量でも液体試料９に試薬８が十分に浸かる構成をとることができるため、液体試料９が微量であっても試薬８を十分に溶解し混合することができる。 Furthermore, the length of the inclined wall surfaces 5a and 5b needs to extend in the inner circumferential direction from the liquid surface 13 so that the liquid sample 9 can move sufficiently during the swinging operation.
As described above, in the present embodiment, by forming the inclined wall surfaces 5a and 5b extending at a predetermined angle so that the tip portion 5c is formed in the outer peripheral direction of the operation chamber 5, the swinging operation is performed in the rotational direction. Since the liquid sample 9 can move sufficiently in the operation chamber 5 and the reagent 8 can be sufficiently immersed in the liquid sample 9 even with a small amount of liquid, the reagent 8 can be used even if the amount of the liquid sample 9 is very small. Can be sufficiently dissolved and mixed.

また、先端部５ｃは尖っていると液体試料９が先端にいくらか付着し、揺動動作を行っても動かないため、角を取り除き、先端部５ｃを曲面とするとなお良い。具体的には、操作チャンバー５の先端部５ｃのＲ寸法は、深さ１２の３ｍｍに対して、Ｒ半径が１ｍｍ〜３ｍｍが良好であった。なお、これは液量やチャンバーの深さや形状や表面状態に応じて任意に変更が可能である。   Further, if the tip 5c is sharp, the liquid sample 9 is somewhat attached to the tip and does not move even if the swinging operation is performed. Therefore, it is better to remove the corner and make the tip 5c curved. Specifically, the R radius of the tip portion 5c of the operation chamber 5 was good when the R radius was 1 mm to 3 mm with respect to the depth 12 of 3 mm. In addition, this can be arbitrarily changed according to the liquid amount, the depth, shape and surface state of the chamber.

さらに効率よく攪拌を行うためには、操作チャンバー５の壁面５ａ，５ｂに撥水処理を行う。撥水処理の方法として、操作チャンバー５内に撥水剤をコーティング、蒸着させる方法がある。また、撥水効果を得る方法として、ベース基板３の材質にポリプロピレン、ポリエチレン、フッ素樹脂等の撥水性材料を用いることが有効である。   In order to perform stirring more efficiently, water repellent treatment is performed on the wall surfaces 5a and 5b of the operation chamber 5. As a water repellent treatment method, there is a method of coating and vapor-depositing a water repellent in the operation chamber 5. Further, as a method for obtaining the water repellent effect, it is effective to use a water repellent material such as polypropylene, polyethylene, or fluororesin as the material of the base substrate 3.

特に、傾斜した壁面５ａ，５ｂとその近辺に撥水処理を行うことで、操作チャンバー５の壁面と液体試料９の表面張力Ｅが下がり、より少ない加速度Ａで攪拌を行うことができる。   In particular, by performing water-repellent treatment on the inclined wall surfaces 5a and 5b and the vicinity thereof, the surface tension E of the wall surface of the operation chamber 5 and the liquid sample 9 is lowered, and stirring can be performed with a smaller acceleration A.

さらに、試薬８内に界面活性剤を含めていることで、操作チャンバー５の壁面と液体試料９の表面張力Ｅが下がり、より少ない力で攪拌できる。
さらに、液体試料９内に界面活性剤を含めていることで、操作チャンバー５の壁面と液体試料９の表面張力Ｅが下がり、より小さな加速度Ａで攪拌できる。 Further, by including a surfactant in the reagent 8, the surface tension E of the wall surface of the operation chamber 5 and the liquid sample 9 is lowered, and stirring can be performed with less force.
Further, by including the surfactant in the liquid sample 9, the surface tension E of the wall surface of the operation chamber 5 and the liquid sample 9 is lowered, and the liquid sample 9 can be stirred with a smaller acceleration A.

図２において分析装置１００は、次のように構成されている。
この分析装置１００は、回転テーブル１０を駆動する回転駆動部１２と、分析容器１内の溶液を光学的に測定するための光学測定手段１４と、回転テーブル１０の回転速度や回転方向および光学測定手段の測定タイミングなどを制御する制御手段１５と、光学測定手段１４によって得られた信号を処理し測定結果を演算するための演算部１６と、演算部１６で得られた結果を表示する表示部１７とで構成されている。光学測定手段１４には、操作チャンバー５の先端部５ｃにレーザー光を照射するためのレーザー光源１８と、レーザー光源１８から照射されたレーザー光のうち、分析容器１を通過した透過光の光量を検出するフォトディテクタ１９とを備えている。 In FIG. 2, the analyzing apparatus 100 is configured as follows.
The analyzer 100 includes a rotation driving unit 12 that drives the rotary table 10, an optical measurement unit 14 that optically measures a solution in the analysis container 1, a rotation speed and a rotation direction of the rotary table 10, and optical measurement. A control unit 15 for controlling the measurement timing of the unit, a calculation unit 16 for processing a signal obtained by the optical measurement unit 14 and calculating a measurement result, and a display unit for displaying the result obtained by the calculation unit 16 17. The optical measuring means 14 includes the laser light source 18 for irradiating the tip 5c of the operation chamber 5 with laser light, and the amount of transmitted light that has passed through the analysis container 1 out of the laser light emitted from the laser light source 18. And a photo detector 19 for detection.

本発明にかかる分析容器は、微量な液体試料を分析する際に必要な分析試薬との混合攪拌ができる方法を有し、分析機器のなかでも、微量な液体試料を分析する装置に有用である。   The analysis container according to the present invention has a method capable of mixing and stirring with an analysis reagent necessary for analyzing a small amount of liquid sample, and is useful for an apparatus for analyzing a small amount of liquid sample among analytical instruments. .

本発明の実施の形態１における分析容器の正面図Front view of analysis container according to Embodiment 1 of the present invention 本発明の実施の形態１における攪拌装置の構成図Configuration diagram of a stirring device in Embodiment 1 of the present invention 本発明の実施の形態１における操作チャンバーの斜視図The perspective view of the operation chamber in Embodiment 1 of this invention 本発明の実施の形態１における揺動動作時の液体試薬にかかる力を示した図The figure which showed the force concerning the liquid reagent at the time of rocking | fluctuation operation in Embodiment 1 of this invention 従来例の説明図Illustration of conventional example

符号の説明Explanation of symbols

１ 分析容器
２ ベース基板
３ カバー基板
４ 液体収容チャンバー
５ 操作チャンバー
５ａ，５ｂ 壁面
５ｃ 操作チャンバー５の先端部
６ 連結流路
７ａ，７ｂ 空気穴
８ 試薬
９ 液体試料
１０ 回転テーブル（回転体）
１１ 回転軸心
１２ 回転駆動部
１３ 液面
１４ 光学測定手段
１５ 制御手段
１６ 演算部
１７ 表示部
１８ レーザー光源
１９ フォトディテクタ
Ａ 加速度
Ｂ 遠心力
Ｃ，Ｄ 分力
Ｅ 表面張力
θ 液面−壁面角
１００ 分析装置 DESCRIPTION OF SYMBOLS 1 Analysis container 2 Base substrate 3 Cover substrate 4 Liquid storage chamber 5 Operation chamber 5a, 5b Wall surface 5c The front-end | tip part 6 of the operation chamber 5 Connection flow path 7a, 7b Air hole 8 Reagent 9 Liquid sample 10 Rotary table (rotary body)
DESCRIPTION OF SYMBOLS 11 Rotation center 12 Rotation drive part 13 Liquid level 14 Optical measurement means 15 Control means 16 Calculation part 17 Display part 18 Laser light source 19 Photodetector A Acceleration B Centrifugal force C, D Component force E Surface tension (theta) Liquid surface-wall angle 100 Analysis apparatus

Claims (6)

液体試料が流れ込む操作チャンバーを有し、揺動動作中の加速度によって前記操作チャンバー内の前記液体試料を攪拌する分析容器であって、
前記操作チャンバーの内周壁の形状を、前記揺動動作中の内周側から最外周位置に向かって先すぼまりの傾斜した壁面で形成した
分析容器。 An analysis container having an operation chamber into which a liquid sample flows, and stirring the liquid sample in the operation chamber by acceleration during a swinging operation,
An analysis container in which the shape of the inner peripheral wall of the operation chamber is formed by an inclined wall surface tapered toward the outermost peripheral position from the inner peripheral side during the swinging operation. 前記操作チャンバーの内周壁の前記最外周位置の形状が、円弧形状である
請求項１に記載の分析容器。 The analysis container according to claim 1, wherein the shape of the outermost peripheral position of the inner peripheral wall of the operation chamber is an arc shape. 前記操作チャンバー内の内周面が撥水処理されている
請求項１記載の分析容器。 The analysis container according to claim 1, wherein an inner peripheral surface in the operation chamber is subjected to water repellent treatment. 前記操作チャンバー内に界面活性剤を含んだ試薬を設けたことを特徴とする
請求項１記載の分析容器。 The analysis container according to claim 1, wherein a reagent containing a surfactant is provided in the operation chamber. 試料液が採取された請求項１記載の分析容器がセットされる分析装置であって、
回転軸芯を持ち前記分析容器を保持する回転体と、
前記分析容器に遠心力が作用するように前記回転体を回転させる回転駆動部と、
前記分析容器の前記操作チャンバー内の液体にアクセスして測定する測定手段と
を有する分析装置。 An analysis apparatus in which the analysis container according to claim 1 in which a sample solution is collected is set,
A rotating body having a rotation axis and holding the analysis container;
A rotation drive unit that rotates the rotating body so that centrifugal force acts on the analysis container;
An analyzer having a measuring means for accessing and measuring the liquid in the operation chamber of the analysis container. 前記回転駆動部は、前記分析容器に保持された前記試料液に働く表面張力よりも大きい遠心力を発生させることを特徴とする
請求項５記載の分析装置。 The analyzer according to claim 5, wherein the rotation driving unit generates a centrifugal force larger than a surface tension acting on the sample solution held in the analysis container.
JP2007278249A 2007-10-04 2007-10-26 Analysis container and analyzer Active JP5273986B2 (en)

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JP2007278249A JP5273986B2 (en) 2007-10-26 2007-10-26 Analysis container and analyzer
US12/681,493 US8415140B2 (en) 2007-10-04 2008-10-03 Analysis device, and analysis apparatus and method using the same
EP18188222.6A EP3447494B1 (en) 2007-10-04 2008-10-03 Analysis method using analysis device
PCT/JP2008/002779 WO2009044552A1 (en) 2007-10-04 2008-10-03 Analysis device, and analysis apparatus and method using the same
CN2008801022246A CN101796420B (en) 2007-10-04 2008-10-03 Analysis device, and analysis apparatus and method using the same
CN201210356041.5A CN102879558B (en) 2007-10-04 2008-10-03 The analytical equipment of analysis instrument and this analysis instrument of use and analytical approach
CN201310374415.0A CN103424543B (en) 2007-10-04 2008-10-03 Analysis method using analysis device
EP16195057.1A EP3141901B1 (en) 2007-10-04 2008-10-03 Analysis device, and analysis apparatus and method using the same
EP08836397.3A EP2209008B1 (en) 2007-10-04 2008-10-03 Analysis device, and analysis apparatus and method using the same
US13/770,499 US8956879B2 (en) 2007-10-04 2013-02-19 Analysis device and method using the same

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