JP2007511230A - 細胞培養システム - Google Patents
細胞培養システム Download PDFInfo
- Publication number
- JP2007511230A JP2007511230A JP2006540322A JP2006540322A JP2007511230A JP 2007511230 A JP2007511230 A JP 2007511230A JP 2006540322 A JP2006540322 A JP 2006540322A JP 2006540322 A JP2006540322 A JP 2006540322A JP 2007511230 A JP2007511230 A JP 2007511230A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- bioreactor
- tank
- bubble
- aeration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/406—Mixers using gas or liquid agitation, e.g. with air supply tubes in receptacles with gas supply only at the bottom
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2215/00—Auxiliary or complementary information in relation with mixing
- B01F2215/04—Technical information in relation with mixing
- B01F2215/0413—Numerical information
- B01F2215/0418—Geometrical information
- B01F2215/0431—Numerical size values, e.g. diameter of a hole or conduit, area, volume, length, width, or ratios thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
本発明の大豆細胞を増殖させる能力は、バッチ培養を用いて実証した。これは、より大きいスケールにおいても、エルレンマイヤーフラスコ又は撹拌槽バイオリアクターに匹敵する又はより優れている。
植物細胞を増殖させるのに優しい環境を与える
スケールアップが容易である
使い捨てである
操作が容易である
Claims (9)
- 液体培地中で生細胞を培養するためのバイオリアクターであって、
細胞及び液体培地を含む少なくとも1個の定置タンクと、
容器の底に単独の大きな気泡を導入するための少なくとも1つの手段と
を含み、
単独の大泡の幅がタンク幅の50〜99%、好ましくは60〜99%、より好ましくは98.5%となるバイオリアクター。 - 単独の大泡の体積が少なくとも65cm3である、請求項1に記載のバイオリアクター。
- バイオリアクターが少なくとも大泡の体積及び頻度をプログラミングするための手段も含む、請求項1及び2に記載のバイオリアクター。
- タンクが、柔軟性がある又はないプラスチック袋である、請求項1から3までに記載のバイオリアクター。
- 定置タンクが硬い外側容器によって囲まれている、請求項1から4までに記載のバイオリアクター。
- タンクの上部が広げられている、請求項1から5までに記載のバイオリアクター。
- タンクが円柱状又は楕円形の断面を有している、請求項1から6までに記載のバイオリアクター。
- 細胞が植物、動物細胞又は微生物である、請求項1から7までに記載のバイオリアクターの使用。
- 細胞がバイオマス細胞、胚形成植物細胞、代謝産物、2次植物代謝物、及び/又は組換え分子を産生している、請求項1から8までに記載のバイオリアクターの使用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP03026377 | 2003-11-18 | ||
EP03026377.6 | 2003-11-18 | ||
PCT/EP2004/013082 WO2005049785A1 (en) | 2003-11-18 | 2004-11-18 | Cell culture system |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007511230A true JP2007511230A (ja) | 2007-05-10 |
JP4845737B2 JP4845737B2 (ja) | 2011-12-28 |
Family
ID=34610052
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006540322A Expired - Fee Related JP4845737B2 (ja) | 2003-11-18 | 2004-11-18 | 細胞培養システム |
Country Status (13)
Country | Link |
---|---|
US (1) | US7897390B2 (ja) |
EP (1) | EP1687394B1 (ja) |
JP (1) | JP4845737B2 (ja) |
CN (1) | CN100523167C (ja) |
AT (1) | ATE470698T1 (ja) |
AU (2) | AU2004291686A1 (ja) |
CA (1) | CA2550745A1 (ja) |
DE (1) | DE602004027651D1 (ja) |
EA (1) | EA008157B1 (ja) |
IL (1) | IL175637A (ja) |
MX (1) | MXPA06005542A (ja) |
NO (1) | NO20062783L (ja) |
WO (1) | WO2005049785A1 (ja) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9005971B2 (en) | 2005-04-22 | 2015-04-14 | Life Technologies Corporation | Gas spargers and related container systems |
US9376655B2 (en) | 2011-09-29 | 2016-06-28 | Life Technologies Corporation | Filter systems for separating microcarriers from cell culture solutions |
US9643133B2 (en) | 2011-09-30 | 2017-05-09 | Life Technologies Corporation | Container with film sparger |
WO2017115722A1 (ja) * | 2015-12-28 | 2017-07-06 | キリン株式会社 | 植物の大量生産方法、及び大量生産設備、並びにそれらに用いる培養袋 |
US10123940B2 (en) | 2014-06-26 | 2018-11-13 | Advanced Scientific, Inc. | Bag assembly and system for use with a fluid |
US10589197B2 (en) | 2016-12-01 | 2020-03-17 | Life Technologies Corporation | Microcarrier filter bag assemblies and methods of use |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL1029124C2 (nl) * | 2005-05-25 | 2006-11-28 | Friesland Brands Bv | Dispensersysteem met gasinjectie. |
DE102006018824A1 (de) | 2006-04-22 | 2007-10-25 | Bayer Technology Services Gmbh | Einweg-Bioreaktor |
DE102006020706B4 (de) * | 2006-05-03 | 2008-08-21 | Sartorius Stedim Biotech Gmbh | Schikanenanordnung für Bioreaktoren |
DE102009005962A1 (de) | 2009-01-23 | 2010-07-29 | Bayer Technology Services Gmbh | Begasungssystem |
DE102009019697A1 (de) * | 2009-05-05 | 2010-11-18 | Bayer Technology Services Gmbh | Container |
GB201004666D0 (en) * | 2010-03-19 | 2010-05-05 | The Technology Partnership Plc | Apparatus for cell culture |
CN102337212B (zh) * | 2011-09-30 | 2013-09-11 | 吴晓春 | 气动振荡式细胞培养*** |
EP2690166A1 (en) * | 2012-07-25 | 2014-01-29 | Mecánica Logroñesa 71, S.L. | Fermentation method and apparatus |
CN105274046A (zh) * | 2014-07-21 | 2016-01-27 | 上海中信国健药业股份有限公司 | 一种大规模动物细胞培养液和微生物发酵液的低温保存方法 |
CN104805013A (zh) * | 2015-05-25 | 2015-07-29 | 固元本草汉方生物科技股份有限公司 | 一次性气升式植物根部组织培养的反应器 |
EP3190170A1 (en) * | 2015-09-30 | 2017-07-12 | Subitec Gmbh | Bioreactor with separate co2 supply |
BR112020015593A2 (pt) * | 2017-12-04 | 2021-01-05 | Synthetic Genomics, Inc. | Fotobiorreator para cultivo de micro-organismos contidos |
WO2019209805A1 (en) | 2018-04-27 | 2019-10-31 | Baxter International Inc. | Method of mixing a pharmaceutical solution and mixing system |
ES2746033A1 (es) * | 2018-09-04 | 2020-03-04 | Univ Santiago Compostela | Sistema para el cultivo de organoides |
CN113348240A (zh) * | 2019-01-04 | 2021-09-03 | 生物技术食品有限公司 | 生物反应器和使用该生物反应器的贴壁细胞培养物的生产方法 |
US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
CN113564125B (zh) * | 2021-08-04 | 2023-01-24 | 河南省肿瘤医院 | 一种用于培养肿瘤细胞的方法 |
EP4253515A1 (en) * | 2022-04-01 | 2023-10-04 | Samabriva | Optimized hairy roots bioreactor |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61212283A (ja) * | 1985-03-15 | 1986-09-20 | ベルコ グラース,インコーポレーテッド | 気送バイオリアクター |
JPH0649143B2 (ja) * | 1984-02-06 | 1994-06-29 | イ−. パ−クス,リチヤ−ド | 容器内で気泡により液体を混合及びブレンドする方法及び装置 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4136970A (en) * | 1977-12-15 | 1979-01-30 | Coulter Electronics, Inc. | Method and apparatus for regulating the size and frequency of bubbles employed for mixing liquids |
EP0258795B1 (en) | 1986-08-27 | 1993-11-03 | Kawasumi Laboratories, Inc. | A method for cultivating cells and an instrument therefor |
EP0471947A1 (en) | 1990-06-29 | 1992-02-26 | Sekisui Chemical Co., Ltd. | Culture bag |
US6190913B1 (en) | 1997-08-12 | 2001-02-20 | Vijay Singh | Method for culturing cells using wave-induced agitation |
US6432698B1 (en) | 1999-01-06 | 2002-08-13 | Rutgers, The State University | Disposable bioreactor for culturing microorganisms and cells |
-
2004
- 2004-11-18 CA CA002550745A patent/CA2550745A1/en not_active Abandoned
- 2004-11-18 EP EP04818800A patent/EP1687394B1/en not_active Not-in-force
- 2004-11-18 MX MXPA06005542A patent/MXPA06005542A/es active IP Right Grant
- 2004-11-18 CN CNB2004800392229A patent/CN100523167C/zh not_active Expired - Fee Related
- 2004-11-18 US US10/595,888 patent/US7897390B2/en not_active Expired - Fee Related
- 2004-11-18 AU AU2004291686A patent/AU2004291686A1/en not_active Abandoned
- 2004-11-18 EA EA200600983A patent/EA008157B1/ru not_active IP Right Cessation
- 2004-11-18 DE DE602004027651T patent/DE602004027651D1/de active Active
- 2004-11-18 WO PCT/EP2004/013082 patent/WO2005049785A1/en active Application Filing
- 2004-11-18 AT AT04818800T patent/ATE470698T1/de not_active IP Right Cessation
- 2004-11-18 JP JP2006540322A patent/JP4845737B2/ja not_active Expired - Fee Related
-
2006
- 2006-05-16 IL IL175637A patent/IL175637A/en not_active IP Right Cessation
- 2006-06-14 NO NO20062783A patent/NO20062783L/no not_active Application Discontinuation
-
2010
- 2010-10-29 AU AU2010238548A patent/AU2010238548B2/en not_active Ceased
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0649143B2 (ja) * | 1984-02-06 | 1994-06-29 | イ−. パ−クス,リチヤ−ド | 容器内で気泡により液体を混合及びブレンドする方法及び装置 |
JPS61212283A (ja) * | 1985-03-15 | 1986-09-20 | ベルコ グラース,インコーポレーテッド | 気送バイオリアクター |
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10328404B2 (en) | 2005-04-22 | 2019-06-25 | Life Technologies Corporation | Gas spargers and related container systems |
US9259692B2 (en) | 2005-04-22 | 2016-02-16 | Life Technologies Corporation | Gas spargers and related container systems |
US9475012B2 (en) | 2005-04-22 | 2016-10-25 | Life Technologies Corporation | Gas spargers and related container systems |
US9005971B2 (en) | 2005-04-22 | 2015-04-14 | Life Technologies Corporation | Gas spargers and related container systems |
US9682353B2 (en) | 2005-04-22 | 2017-06-20 | Life Technologies Corporation | Gas spargers and related container systems |
US9376655B2 (en) | 2011-09-29 | 2016-06-28 | Life Technologies Corporation | Filter systems for separating microcarriers from cell culture solutions |
US11840684B2 (en) | 2011-09-29 | 2023-12-12 | Life Technologies Corporation | Filter systems for separating microcarriers from cell culture solutions |
US10934514B2 (en) | 2011-09-29 | 2021-03-02 | Life Technologies Corporation | Filter systems for separating microcarriers from cell culture solutions |
US10301585B2 (en) | 2011-09-29 | 2019-05-28 | Life Technologies Corporation | Filter systems for separating microcarriers from cell culture solutions |
US9643133B2 (en) | 2011-09-30 | 2017-05-09 | Life Technologies Corporation | Container with film sparger |
US10350554B2 (en) | 2011-09-30 | 2019-07-16 | Life Technologies Corporation | Container with film Sparger |
US10843141B2 (en) | 2011-09-30 | 2020-11-24 | Life Technologies Corporation | Container with film sparger |
US10463571B2 (en) | 2014-06-26 | 2019-11-05 | Advanced Scientifics, Inc. | Bag assembly and bag system for use with a fluid |
US10123940B2 (en) | 2014-06-26 | 2018-11-13 | Advanced Scientific, Inc. | Bag assembly and system for use with a fluid |
WO2017115722A1 (ja) * | 2015-12-28 | 2017-07-06 | キリン株式会社 | 植物の大量生産方法、及び大量生産設備、並びにそれらに用いる培養袋 |
US10589197B2 (en) | 2016-12-01 | 2020-03-17 | Life Technologies Corporation | Microcarrier filter bag assemblies and methods of use |
US11344827B2 (en) | 2016-12-01 | 2022-05-31 | Life Technologies Corporation | Microcarrier filter bag assemblies and methods of use |
US11890557B2 (en) | 2016-12-01 | 2024-02-06 | Life Technologies Corporation | Microcarrier filter bag assemblies and methods of use |
Also Published As
Publication number | Publication date |
---|---|
EA008157B1 (ru) | 2007-04-27 |
ATE470698T1 (de) | 2010-06-15 |
EA200600983A1 (ru) | 2006-10-27 |
CA2550745A1 (en) | 2005-06-02 |
AU2004291686A1 (en) | 2005-06-02 |
CN100523167C (zh) | 2009-08-05 |
EP1687394A1 (en) | 2006-08-09 |
AU2010238548B2 (en) | 2011-09-22 |
US20070037279A1 (en) | 2007-02-15 |
CN1902304A (zh) | 2007-01-24 |
IL175637A (en) | 2010-12-30 |
US7897390B2 (en) | 2011-03-01 |
JP4845737B2 (ja) | 2011-12-28 |
WO2005049785A1 (en) | 2005-06-02 |
MXPA06005542A (es) | 2006-08-23 |
IL175637A0 (en) | 2006-09-05 |
AU2010238548A1 (en) | 2010-11-18 |
EP1687394B1 (en) | 2010-06-09 |
NO20062783L (no) | 2006-08-16 |
DE602004027651D1 (de) | 2010-07-22 |
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