JP2006138853A - 分子相互作用の測定を容易にするための装置及び方法 - Google Patents
分子相互作用の測定を容易にするための装置及び方法 Download PDFInfo
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Abstract
【解決手段】生体分子の相互作用の測定を容易にする方法は、相互作用種用流体ルータ(300)を基板(305)に取り付けるステップを含む。相互作用種のサンプルに対応する複数の反応領域を有する被膜されたセンサ表面(307)を生成するために、1組の相互作用種のサンプルが、相互作用種用流体ルータ(300)によって送出される。次に、相互作用種用流体ルータ(300)が基板から除去される。次に、複数サンプル用流体ルータ(310)が基板(305)に接続される。次に、複数サンプル用流体ルータ(310)によって、1組の流体サンプルが複数の反応領域(308)に送られる。次に、結果として生じた生体分子の相互作用に対応する光信号が収集される。その後、この光信号を処理して生体分子の相互作用の特性を解明する。
【選択図】図2
Description
102 流体ルータ
104 薄膜
106 透明基体
108 流体伝達装置
110 複数チャンバ流体貯蔵部
112 光学装置
114 光源
116 2次元センサ
118 生体分子相互作用の信号処理プロセッサ
300 相互作用種用流体ルータ
305 基体(基板)
307 センサ表面
310 複数試料用流体ルータ
Claims (14)
- 基体と流体連絡する相互作用種用流体ルータを設けるステップと、
前記相互作用種用流体ルータによって1組の相互作用種の試料を送り、前記相互作用種の試料に対応する複数の反応ゾーンを有する被覆されたセンサ表面を生成するステップ
を含む、方法。 - さらに、
前記基体から前記相互作用種用流体ルータを除去するステップと、
前記基体に複数試料用流体ルータを接続するステップと、
前記複数試料用流体ルータによって前記複数の反応ゾーンに複数の流体試料を送るステップ
を含む、請求項1に記載の方法。 - 1組の相互作用種の試料を送る前記ステップに、前記相互作用種用流体ルータによって1組の異種の相互作用種の試料を送って、前記異種の相互作用種の試料に対応する複数の異種の反応ゾーンを有する被覆されたセンサ表面を生成するステップが含まれる、請求項2に記載の方法。
- 前記接続するステップに、前記相互作用種用流体ルータの配向に対して直交する配向をなすように、前記複数試料用流体ルータを接続するステップが含まれる、請求項2に記載の方法。
- 複数の流体試料を送る前記ステップに、単一のソースから前記複数の反応ゾーンに複数の流体試料を送るステップが含まれる、請求項2に記載の方法。
- 複数の流体試料を送る前記ステップに、複数のソースから前記複数の反応ゾーンに複数の流体試料を送るステップが含まれる、請求項2に記載の方法。
- 複数の反応ゾーンを有する基体と、
前記基体の前記複数の反応ゾーンに複数の流体試料を送るための複数試料用流体ルータ
を備える、生体分子相互作用測定用流体システム。 - 前記複数の反応ゾーンによって、複数の異種の流体試料と相互作用するための単一の相互作用種が画定される、請求項7に記載の流体システム。
- 前記複数の反応ゾーンに、単一の流体試料と相互作用するための複数の相互作用種が含まれる、請求項7に記載の流体システム。
- 前記複数の反応ゾーンに、複数の異種の流体試料と相互作用するための複数の相互作用種が含まれる、請求項7に記載の流体システム。
- 前記複数試料用流体ルータに、垂直ポートを備えた流体ポート・アレイが含まれる、請求項7に記載の流体システム。
- 前記複数試料用流体ルータに、水平ポートを備えた流体ポート・アレイが含まれる、請求項7に記載の流体システム。
- 前記複数試料用流体ルータに、水平ポート及び垂直ポートを備えた流体ポート・アレイが含まれる、請求項7に記載の流体システム。
- 前記複数試料用流体ルータに、前記複数の反応ゾーンに対応する1組の流体輸送管が含まれ、各流体輸送管に、入口流路及び出口流路を画定する同軸管が含まれる、請求項7に記載の流体システム。
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US10/987,991 US20060106548A1 (en) | 2004-11-12 | 2004-11-12 | Apparatus and method for facilitating molecular interaction measurements |
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Publication Number | Publication Date |
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JP2006138853A true JP2006138853A (ja) | 2006-06-01 |
JP2006138853A5 JP2006138853A5 (ja) | 2008-12-25 |
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US (1) | US20060106548A1 (ja) |
EP (1) | EP1657549A1 (ja) |
JP (1) | JP2006138853A (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011257266A (ja) * | 2010-06-09 | 2011-12-22 | Konica Minolta Holdings Inc | 光学センサおよび光学センサに用いられるチップ構造体 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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FR2941531B1 (fr) * | 2009-01-26 | 2019-07-05 | Phuong Lan TRAN | Dispositif microfluidique, systeme et procede de mise en oeuvre |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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SE9700384D0 (sv) * | 1997-02-04 | 1997-02-04 | Biacore Ab | Analytical method and apparatus |
US6192168B1 (en) * | 1999-04-09 | 2001-02-20 | The United States Of America As Represented By The Secretary Of The Navy | Reflectively coated optical waveguide and fluidics cell integration |
CN1137999C (zh) * | 2000-07-04 | 2004-02-11 | 清华大学 | 集成式微阵列装置 |
-
2004
- 2004-11-12 US US10/987,991 patent/US20060106548A1/en not_active Abandoned
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2005
- 2005-11-10 JP JP2005325865A patent/JP2006138853A/ja active Pending
- 2005-11-14 EP EP05257008A patent/EP1657549A1/en not_active Ceased
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011257266A (ja) * | 2010-06-09 | 2011-12-22 | Konica Minolta Holdings Inc | 光学センサおよび光学センサに用いられるチップ構造体 |
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US20060106548A1 (en) | 2006-05-18 |
EP1657549A1 (en) | 2006-05-17 |
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