JP2004313178A - Method for increasing 5'-nucleotide content in mother's milk of mother sow, additive to feed for mother sow and feed for mother sow - Google Patents
Method for increasing 5'-nucleotide content in mother's milk of mother sow, additive to feed for mother sow and feed for mother sow Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 32
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- 239000000654 additive Substances 0.000 title 1
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- IERHLVCPSMICTF-XVFCMESISA-N cytidine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-XVFCMESISA-N 0.000 claims abstract description 6
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- 229950006790 adenosine phosphate Drugs 0.000 claims abstract description 5
- RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 claims abstract description 5
- 235000013928 guanylic acid Nutrition 0.000 claims abstract description 5
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- DJJCXFVJDGTHFX-UHFFFAOYSA-N Uridinemonophosphate Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-UHFFFAOYSA-N 0.000 claims abstract description 4
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Abstract
Description
本発明は、リボ核酸及び／又は５’−ヌクレオチドを含有する酵母エキスを母豚に投与する母豚の母乳中の５’−ヌクレオチド含有量を増加させる方法、及びリボ核酸及び／又は５’−ヌクレオチドを含有する酵母エキスを含む飼料添加物及び飼料に関する。 The present invention relates to a method for increasing the 5′-nucleotide content in breast milk of a sow, wherein a yeast extract containing ribonucleic acid and / or 5′-nucleotide is administered to the sow, and ribonucleic acid and / or 5′-. The present invention relates to a feed additive containing a yeast extract containing nucleotides and a feed.
一般に、家畜の幼若期は免疫機能が十分でなく、トラブル、例えば消化管系あるいは呼吸器系の感染症等、が発生しやすい。特に、豚は、出生児の成熟度が低く、他の家畜よりも生理的に未熟であり、授乳期に下痢等に罹患し、それが原因で死亡したり、死亡に至らないまでもその後の成長、特に体重増加に悪影響を与えることが知られている。
これらは経済的に大きな損失をもたらすことから、分娩前の母豚の全身を細菌感染防止処理液で噴霧・塗布し母豚からの細菌の感染を防止する方法（特許文献１）、あるいは妊娠又は授乳期の母豚に、カラクトオリゴ糖、細菌の菌体あるいは菌体粉砕物、あるいは還元型葉酸等（特許文献２）を投与し、母豚を通して子豚に投与する方法、等の予防方法が報告されている。
しかしながら、これらの効果は限定的であり、十分に満足のいくものではなかった。
In general, the immune function is insufficient during the juvenile period of livestock, and troubles such as infections of the digestive tract or respiratory system are likely to occur. In particular, pigs have low birth maturity, are physiologically immature than other livestock, suffer from diarrhea, etc. during the lactation period. It is known to adversely affect growth, especially weight gain.
Since these bring about a large loss economically, the method (patent document 1) which sprays and apply | coats the whole body of the mother pig before a delivery with a bacterial infection prevention process liquid, and prevents the infection of the bacteria from a mother pig, or pregnancy or Reported preventive methods such as administering calactooligosaccharides, bacterial cells or pulverized bacterial bodies, or reduced folic acid (Patent Document 2) to lactating sows and administering them to the piglets through the sows Has been.
However, these effects were limited and not fully satisfactory.
ヌクレオチド、核酸類は、摂餌促進作用あるいは腸管の絨毛組織回復作用を有するといわれており、５’−ヌクレオチドを含有した酵母エキス（特許文献３）あるいは核酸（特許文献４）を子豚に投与し、増体を促進したり飼料効率を改善し、下痢等にかかりやすい幼若期の期間を短縮する方法が報告されている。
しかしながら、これらはいずれも子豚に投与するものであり、また離乳豚が主として対象となるもので、特に問題となる授乳期、出生後１０日前後まで、の下痢あるいは虚弱等を改善するものではなかった。
However, these are all administered to piglets, and are mainly intended for weaning pigs. In particular, they do not improve diarrhea or frailty until about 10 days after birth, especially during lactation. There wasn't.
本発明は、成熟度が低く抵抗性の弱い子豚、特に出生から授乳期の子豚、の下痢、虚弱を防止する、経済的に安価でかつ有効な手段を提供することを課題とする。 An object of the present invention is to provide an economically inexpensive and effective means for preventing diarrhea and frailty of a piglet with low maturity and weak resistance, particularly a piglet from birth to lactation.
本発明は、リボ核酸及び／又は５’−ヌクレオチドを含有する酵母エキスを母豚に投与することを最も主要な特徴とする。
すなわち、本発明は、
（１）リボ核酸及び／又は５’−ヌクレオチドを含有する酵母エキスを母豚に投与することを特徴とする、母豚の母乳中の５’−ヌクレオチド含有量を増加させる方法、
（２）リボ核酸を含有する酵母エキスが、リボ核酸を３〜６０重量％含有したものである、上記（１）記載の母豚の母乳中の５’−ヌクレオチド含有量を増加させる方法、
（３）５’−ヌクレオチドを含有する酵母エキスが、５’−グアニル酸、５’−アデニル酸、５’−シチジル酸及び５’−ウリジル酸、あるいはそれらの塩を、それぞれ１〜１５重量％含有したものである、請求項１記載の母豚の母乳中の５’−ヌクレオチド含有量を増加させる方法、
（４）リボ核酸及び／又は５’−ヌクレオチドを含有する酵母エキスを有効成分とする母豚用飼料添加物、
（５）リボ核酸及び／又は５’−ヌクレオチドを含有する酵母エキスを有効成分とする母豚用飼料添加物を含有した母豚用飼料、
を提供するものである。
The main feature of the present invention is that a yeast extract containing ribonucleic acid and / or 5′-nucleotide is administered to a sow.
That is, the present invention
(1) A method for increasing the 5′-nucleotide content in the breast milk of a mother pig, which comprises administering a yeast extract containing ribonucleic acid and / or 5′-nucleotide to the mother pig,
(2) The method for increasing the 5′-nucleotide content in the breast milk of sows according to (1) above, wherein the yeast extract containing ribonucleic acid contains 3 to 60% by weight of ribonucleic acid,
(3) The yeast extract containing 5′-nucleotide contains 1 to 15% by weight of 5′-guanylic acid, 5′-adenylic acid, 5′-cytidylic acid and 5′-uridylic acid, or salts thereof, respectively. The method for increasing the 5′-nucleotide content in the breast milk of the sow according to claim 1,
(4) Feed additive for sows containing yeast extract containing ribonucleic acid and / or 5′-nucleotide as an active ingredient,
(5) A feed for sows containing a feed additive for sows containing a yeast extract containing ribonucleic acid and / or 5'-nucleotide as an active ingredient,
Is to provide.
本発明は、経済的に安価で極めて簡単な方法で、母豚の母乳中の５’−ヌクレオチド含有量を増加させることができるため、成熟度が低く抵抗性の弱い子豚、特に出生から授乳期の子豚、の下痢及び虚弱を有効に防止できる。 The present invention is able to increase the 5'-nucleotide content in the mother's milk in an economically cheap and extremely simple manner, so that pigs with low maturity and weak resistance, particularly breastfeeding. Can effectively prevent diarrhea and frailty at the end of the season.
以下、本発明を詳細に説明する。
本発明の有効成分であるリボ核酸あるいは５’−ヌクレオチドを含有する酵母エキスは、リボ核酸、あるいは５’−グアニル酸、５’−アデニル酸、５’−イノシン酸、５’−シチジル酸、５’−ウリジル酸等の５’−ヌクレオチド類及びそれらのナトリウム塩、カリウム塩、を含有する酵母エキスである。
酵母エキスは、これら化合物を含有するものであれば特に制限はないが、リボ核酸であれば３〜６０重量％、好ましくは１０〜６０重量％、５’−ヌクレオチド類であれば５’−グアニル酸、５’−アデニル酸、５’−シチジル酸、５’−ウリジル酸あるいはそれらの塩をそれぞれ１〜１５重量％、好ましくはそれぞれ３〜１５重量％、含有する酵母エキスが望ましい。
Hereinafter, the present invention will be described in detail.
The yeast extract containing ribonucleic acid or 5′-nucleotide, which is an active ingredient of the present invention, is ribonucleic acid, or 5′-guanylic acid, 5′-adenylic acid, 5′-inosinic acid, 5′-cytidylic acid, 5 It is a yeast extract containing 5'-nucleotides such as' -uridylic acid and their sodium and potassium salts.
The yeast extract is not particularly limited as long as it contains these compounds, but it is 3 to 60% by weight for ribonucleic acid, preferably 10 to 60% by weight, and 5'-guanyl for 5'-nucleotides. Yeast extracts containing 1 to 15% by weight, preferably 3 to 15% by weight, respectively, of acid, 5′-adenylic acid, 5′-cytidylic acid, 5′-uridylic acid or salts thereof are desirable.
リボ核酸あるいは５’−ヌクレオチドを含有する酵母エキスは、公知の方法、例えば、酵母をｐＨ５〜１２の温熱水で抽出する方法、酵母をタンパク質分解酵素、細胞壁分解酵素などを添加して抽出する方法、高圧分散処理等の高圧粉砕により抽出する方法、等の方法により得られるが、これらの中で、酵母を温熱水で抽出した酵母エキス、及び抽出した酵母エキスを核酸分解酵素を作用させることによる得られる酵母エキスが、リボ核酸あるいは５’−ヌクレオチド含有量が高いため、特に好ましい。
酵母エキスは、液状、粉末状のいずれでもよいが、粉末状のものが好ましい。粉末状の酵母エキスは、抽出液を常法により、例えば、濃縮、噴霧乾燥等の方法により容易に製造することができる。
A yeast extract containing ribonucleic acid or 5′-nucleotide is a known method, for example, a method of extracting yeast with hot water having a pH of 5 to 12, a method of extracting yeast by adding a proteolytic enzyme, a cell wall degrading enzyme or the like. It can be obtained by a method such as a high-pressure pulverization method such as high-pressure dispersion treatment, etc. Among them, a yeast extract obtained by extracting yeast with hot water, and a nucleolytic enzyme on the extracted yeast extract. The resulting yeast extract is particularly preferred because of its high ribonucleic acid or 5′-nucleotide content.
The yeast extract may be either liquid or powder, but is preferably powder. The powdered yeast extract can be easily produced by a conventional method, for example, a method such as concentration or spray drying.
これら酵母エキスに使用される酵母は、食品あるいは食品添加物として用いられている酵母で、例えば、パン酵母、ビール酵母、トルラ酵母などを挙げることができる。 Yeasts used in these yeast extracts are yeasts used as foods or food additives, and examples thereof include baker's yeast, brewer's yeast, and torula yeast.
本発明のリボ核酸あるいは５’−ヌクレオチドを含有する酵母エキスを有効成分とする飼料添加物は、母豚に投与される。
対象となる豚は特に制限はなく、例えば、経済豚としては、ランドレース種、大ヨークシャー種、ジュロック種、バークシャー種、パンプシャー種、ピエトレン種あるいはこれら品種間の交雑種、ミニチュアーピッグとしては、ゲッチンゲン種、ポットベリー種、クラウンミニー種及びこれら品種間の交雑種、等を例示することができる。
投与方法は任意であるが、通常与えている飼料に添加する方法、あるいは他の飼料や水に添加し投与する方法、等を例示することができる。
投与時期は、分娩予定日の１〜２週間前から分娩までで十分であるが、更に授乳期間中も投与することができる。投与は連続的に、あるいは不連続でも良い。
投与量は、豚の種類、用いる酵母エキスあるいは酵母エキス中のリボ核酸、５’−ヌクレオチド含有量等により異なるが、概ね、母豚体重１ｋｇ当たり１日０．０１〜１ｇ（リボ核酸あるいは５’−ヌクレオチドとして０．０００５〜０．６ｇ）、好ましくは０．０２〜０．６ｇ（同、０．００１〜０．３ｇ）程度である。
A feed additive comprising a yeast extract containing the ribonucleic acid or 5′-nucleotide of the present invention as an active ingredient is administered to a sow.
The target pigs are not particularly limited.For example, as an economic pig, a Landrace breed, a large Yorkshire breed, a Jurlock breed, a Berkshire breed, a Pumpshire breed, a Pietren breed, or a hybrid between these breeds, a miniature pig, Examples include Göttingen species, Potberry species, Crown Minnie species and hybrids between these varieties.
The administration method is arbitrary, but examples thereof include a method of adding to a normal feed or a method of adding to another feed or water for administration.
The administration time is sufficient from 1 to 2 weeks before the scheduled delivery date to delivery, but it can also be administered during the lactation period. Administration may be continuous or discontinuous.
The dose varies depending on the type of pig, the yeast extract to be used or the ribonucleic acid in the yeast extract, the 5′-nucleotide content, etc., but is generally 0.01 to 1 g (ribonucleic acid or 5 ′) per kg of the sow body weight per day. -As a nucleotide, it is 0.0005-0.6g), Preferably it is about 0.02-0.6g (same, 0.001-0.3g).
本発明の母豚用飼料添加物は、飼料と配合して母豚用飼料とすることができる。
配合できる飼料としては、通常使用されている母豚用の飼料が望ましい。
配合量は、母豚用飼料中、０．０１〜５重量％、好ましくは０．１〜２重量％程度であり、配合方法は任意であるが、母豚用の飼料に本発明の母豚用飼料添加物を混合する方法が簡便であり好ましい。
The feed additive for sows of the present invention can be blended with feed to make a feed for sows.
As a feed that can be blended, a commonly used feed for mother pigs is desirable.
The blending amount is 0.01 to 5% by weight, preferably about 0.1 to 2% by weight, in the feed for sows, and the blending method is arbitrary. A method of mixing the feed additive for use is simple and preferable.
本発明の特徴は、豚母乳中の５’−ヌクレオチド含有量を増加させる点にある。
本発明者らは、豚母乳中の成分を検討したところ、５’−ヌクレオチドが含まれていること、５’−ヌクレオチド含有量の高い母乳を授乳した子豚は下痢の発生率が低いこと、を見いだし、かかる知見をもとに、母乳中の５’−ヌクレオチド含有量を増加させる方法を検討したところ、分娩前に母豚にリボ核酸あるいは５’−ヌクレオチドを含有する酵母エキスを投与するだけ、という、極めて簡単な方法で、母乳中の５’−ヌクレオチド含有量が増加することを見いだしたものである。
A feature of the present invention is that it increases the 5′-nucleotide content in pig breast milk.
The inventors of the present invention have examined the components in pork breast milk, the 5'-nucleotide is included, the piglet fed breast milk having a high 5'-nucleotide content has a low incidence of diarrhea, Based on this finding, we studied a method to increase the 5'-nucleotide content in breast milk, and only administered yeast extract containing ribonucleic acid or 5'-nucleotide to the mother pig before delivery. The 5′-nucleotide content in breast milk was found to increase in a very simple manner.
以下、実施例を挙げて本発明を具体的に説明する。
なお、実施例に記載の、５’−ヌクレオチド量は、以下の方法により測定した。
（１）豚母乳の前処理
得られた豚母乳１ｍｌに対し、２〜４℃に冷却した０．６４Ｍ過塩素酸２ｍｌを加え、３０秒間攪拌した後、氷水中に１．５時間放置した。放置後、豚母乳懸濁液を冷却遠心分離機（８０００ｒｐｍ、２０分間、４℃）で沈殿物を除き上清液を得た。沈殿物は冷却した０．６４Ｍ過塩素酸１ｍｌで同様に処理して上清液を得た（２回）。得られた上清液を一つに混合し、０．４５μｍのフィルターで濾過した後、高速液体クロマトグラフィー（ＨＰＬＣ）により５’−ヌクレオチドを測定した。
（２）ＨＰＬＣの条件
・分離カラム：ＭＣＩＧＥＬＣＤＲ−１０（６０℃、４．６ｍｍ×２５０ｍｍ、三菱化学製）
・移動相：２Ｍ 酢酸−酢酸ナトリウム緩衝液（ｐＨ３．３）
・流速：２．０ｍｌ／ｍｉｎ
・検出：紫外線検出器（２１０ｎｍ）
Hereinafter, the present invention will be specifically described with reference to examples.
In addition, the 5′-nucleotide amount described in Examples was measured by the following method.
(1) Pretreatment of
(2) HPLC conditions Separation column: MCIGELCDR-10 (60 ° C., 4.6 mm × 250 mm, manufactured by Mitsubishi Chemical)
Mobile phase: 2M acetic acid-sodium acetate buffer (pH 3.3)
・ Flow rate: 2.0ml / min
・ Detection: UV detector (210nm)
試験例 母乳中の５’−ヌクレオチド濃度
全国畜産農業協同組合連合会研修牧場において飼育中の母豚１１頭（ＬＷ種及びＷＬ種）を供試して、母乳中の５’−ヌクレオチド量を測定した。
搾乳は母豚の分娩終了直後から開始し、分娩後４日目までは毎日１回、それぞれの乳頭から用指法で実施した。その後は１４日目及び２２日目に搾乳を実施した。なお、母豚には通常の飼料を給与した。
その結果、母乳中に５’−ヌクレオチドが含まれていることが確認されると共に、５’−ヌクレオチド濃度により、分娩初日に２００μｍｏｌ／１００ｍｌ以上の５’−ヌクレオチドを含む乳を分泌する母豚（４頭、高ヌクレオチド区という）と、５’−ヌクレオチド濃度が１５０μｍｏｌ／１００ｍｌ以下である乳を分泌する母豚（７頭、低ヌクレオチド区という）の２区に分けることができた。
高ヌクレオチド区、低ヌクレオチド区の５’−ヌクレオチド濃度の推移を図１に示す。
両区を比較すると（図１）、分娩後０〜３日目までの高ヌクレオチド区の５’−ヌクレオチド濃度は低ヌクレオチド区に対して有意に高かった（ｐ＜０．０１、ただし分娩後１日目のみｐ＜０．０５）。分娩後４日目以降は両区に有意差はなかった。
産子子豚の離乳時まで（分娩後２６日間）の下痢及び虚弱治療成績（下痢及び虚弱子豚の判断及び治療方法は、研修牧場の観光法に従った。）では、高ヌクレオチド区ではほとんど治療を必要としなかったが、低ヌクレオチド区ではかなりの回数の治療を必要とした。また、産子子豚の死亡原因についても、高ヌクレオチド区では衰弱死はみられなかった（死亡原因は全て圧死）が、低ヌクレオチド区では半数が衰弱死であった。
これらの結果から、母豚母乳中、特に授乳期初期、に含まれる５’−ヌクレオチドは、新生子豚の発育にとって重要な役割をもつことが示唆された。
Test example 5'-nucleotide concentration in breast milk 11 pigs (LW species and WL species) being bred at the National Livestock Agricultural Cooperative Federation training ranch were tested to measure the amount of 5'-nucleotide in breast milk. .
Milking was started immediately after the end of the delivery of the mother pig, and was carried out by finger method from each teat once a day until the fourth day after delivery. Thereafter, milking was performed on the 14th and 22nd days. Mother pigs were fed regular feed.
As a result, it was confirmed that 5′-nucleotide was contained in the breast milk, and the mother pig secreting milk containing 200 μmol / 100 ml or more of 5′-nucleotide on the first day of delivery according to the 5′-nucleotide concentration ( 4 groups, referred to as high nucleotide group) and 2 groups of mother pigs that secrete milk having a 5′-nucleotide concentration of 150 μmol / 100 ml or less (7 groups, referred to as low nucleotide group).
The transition of the 5′-nucleotide concentration in the high nucleotide group and the low nucleotide group is shown in FIG.
Comparing the two groups (FIG. 1), the 5′-nucleotide concentration in the high nucleotide group from
Diagnosis of diarrhea and frailty treatment until weaning of piglets (26 days after parturition) (Judgment and treatment methods for diarrhea and frailty piglets were according to the tourism method of the training ranch). Although no treatment was required, a significant number of treatments were required in the low nucleotide group. In addition, as for the cause of death of the litter and piglet, no debilitating death was observed in the high nucleotide group (the cause of death was all crushing), but half of the deaths were in the low nucleotide group.
From these results, it was suggested that 5′-nucleotide contained in the sow milk, particularly in the early lactation period, has an important role in the development of the newborn piglets.
実施例１
母豚３頭（ポットベリー種、名前：ピンキー、マルコ、モモ）を用い、５’−ヌクレオチド含有酵母エキスを投与した場合（投与区、２００２年１０月試験開始）、及び投与しない場合（無投与区、２００２年６月試験開始）の試験を行った。
すなわち、母豚の分娩予定日前から分娩日までにわたり、表１に示した条件で５’−ヌクレオチド含有酵母エキスを与え、分娩後の母豚母乳中の５’−ヌクレオチド量、及び離乳時まで（分娩後２６日間）の各区毎に下痢した子豚及び虚弱死した子豚の頭数を計測した。なお、本試験においては、下痢及び虚弱治療は実施しなかった。
試験期間中、使用した飼料はパワーマシュ−Ｓ（中部飼料製）で、投与区は５’−ヌクレオチド含有酵母エキス（興人製、酵母エキスＮＴ、５’−ヌクレオチド含有量：５’−グアニル酸ナトリウム１１．１％、５’−アデニル酸ナトリウム１０．６％、５’−シチジル酸ナトリウム７．３％、５’−ウリジル酸ナトリウム７．９％）を当該飼料に混合して用いた。また、搾乳は分娩直後から開始し、２時間目、６時間目、１２時間目及び分娩３日目までは毎日１回、その後は５日目、７日目、１４日目、２１日目及び２８日目に、用指法で搾乳した。
Example 1
When 3 mother pigs (potberry species, names: Pinky, Marco, peach) were used and 5'-nucleotide-containing yeast extract was administered (administration zone, test started in October 2002), and when not administered (no administration) Ward, test started in June 2002).
That is, a 5′-nucleotide-containing yeast extract was given under the conditions shown in Table 1 from the expected delivery date of the sows to the delivery date, and the 5′-nucleotide amount in the sows mother milk after delivery and until weaning ( The number of diarrhea piglets and frailly dead piglets were counted for each section (26 days after parturition). In this study, diarrhea and frailty treatment were not performed.
During the test period, the feed used was Powermash-S (manufactured by Chubu Feed), and the administration group was 5'-nucleotide-containing yeast extract (manufactured by Kojin, yeast extract NT, 5'-nucleotide content: 5'-guanylic acid. Sodium 11.1%, 5′-adenylate sodium 10.6%, 5′-sodium cytidylate 7.3%, 5′-sodium uridylate 7.9%) were mixed with the feed and used. Milking starts immediately after delivery, once every day until the 2nd, 6th, 12th and 3rd day of delivery, then the 5th, 7th, 14th, 21st and On the 28th day, milking was performed using the finger method.
母豚母乳中の５’−ヌクレオチド濃度の推移を、図２（無投与区）及び図３（投与区）に示す。
図２及び図３より、無投与区に比較して投与区では、母乳中の５’−ヌクレオチド濃度が増加していることが分かる。
表２に、離乳時まで（分娩２６日間）の子豚の飼育成績を示す。
The transition of the 5′-nucleotide concentration in the sow milk is shown in FIG. 2 (no administration group) and FIG. 3 (administration group).
2 and 3, it can be seen that the 5′-nucleotide concentration in breast milk increased in the administration group compared to the non-administration group.
Table 2 shows the breeding results of the piglets until weaning (26 days of delivery).
表２から明らかなように、無投与区では下痢が多く、虚弱死した子豚が７頭と半数近くを占めていたが、投与区では下痢は見られず、また、虚弱死した子豚も見られなかった。 As is clear from Table 2, there were many diarrhea in the non-administered group, and nearly half of the piglets that died weakly accounted for almost half, but no diarrhea was observed in the administered group, and there were also frailly dead piglets. I couldn't see it.
製造例１
３０Ｌ容ジャーファーメンターを用い、キャンディダ・ウチルスＫＪＳ−０５８２株（ＦＥＲＭ Ｐ−７３９６株）の前培養液１Ｌを植菌し、３０℃で２４時間通気培養して酵母菌体を得た。培地は、グルコース５重量％、燐酸アンモニウム０．２重量％、硫酸アンモニウム０．２重量％、硫酸マグネシウム０．１重量％、塩化カリウム０．１７重量％、硫酸第二鉄５ｐｐｍ、硫酸亜鉛９ｐｐｍ、塩化マンガン４ｐｐｍを含む水溶液をｐＨ４．５に調整したものである。
培養終了後、シャープレス型遠心分離機にて菌体を回収し、菌体を水で２回洗浄した。
得られた菌体を水で菌体濃度１０％に調整・懸濁した後、９０℃、３０分間加熱し菌体内酵素を失活させ、次いで、６５℃に冷却した。冷却液のｐＨを９とし、同温度で６０分間処理し、エキスを抽出した。遠心分離機により菌体残渣を除去し、得られた上澄液をｐＨ７に中和し、デキストリンを添加後、スプレードライし、リボ核酸含有酵母エキスを得た。
本酵母エキスは、リボ核酸を２７重量％含んでいた。
Production Example 1
Using a 30 L jar fermenter, 1 L of a preculture solution of Candida utilis KJS-0582 strain (FERM P-7396 strain) was inoculated and cultured by aeration at 30 ° C. for 24 hours to obtain yeast cells. The medium was
After completion of the culture, the cells were collected with a sharpless centrifuge, and the cells were washed twice with water.
The obtained bacterial cells were adjusted and suspended with water to a cell concentration of 10%, heated at 90 ° C. for 30 minutes to inactivate intracellular enzymes, and then cooled to 65 ° C. The extract was extracted by adjusting the pH of the cooling liquid to 9 and treating at the same temperature for 60 minutes. The cell residue was removed by a centrifuge, and the resulting supernatant was neutralized to pH 7, added with dextrin and spray-dried to obtain a ribonucleic acid-containing yeast extract.
This yeast extract contained 27% by weight of ribonucleic acid.
実施例２
母豚１頭（ＬＷ種）を用い、リボ核酸含有酵母エキスを投与した場合（投与区、２００３年９月試験開始）、及び投与しない場合（無投与区、２００３年４月試験開始）の試験を行った（於：農事法人組合打戻種豚組合）。
すなわち、母豚の分娩予定日５日前から分娩日までにわたり、表３に示した条件でリボ核酸含有酵母エキスを与え、分娩後の母豚母乳中の５’−ヌクレオチド量、及び離乳時まで（分娩後２６日間）の各区毎に下痢した子豚及び虚弱死した子豚の頭数を計測した。なお、本試験においては、下痢及び虚弱治療は実施しなかった。
試験期間中、使用した飼料はウィニーブリード（日本農産工業製、種豚飼育用配合飼料）で、投与区は製造例１で得られたリボ核酸含有酵母エキスを当該飼料に混合して用いた。また、搾乳は分娩直後から開始し、１時間目、２時間目、３時間目、６時間目、分娩３日目までは毎日１回、その後は７日目、１４日目、２１日目及び２８日目に、用指法で搾乳した。
なお、搾乳時に、搾乳しやすいようにホルモン剤（帝国臓器製薬製、アトニン）１ｍｌを、搾乳の１０分前に筋肉注射した。
Example 2
Tests using one sow (LW species) and administering ribonucleic acid-containing yeast extract (administered group, start of test in September 2003) and not administered (non-administered group, start of test in April 2003) (At: Agricultural corporation union bred sow pig association).
That is, the ribonucleic acid-containing yeast extract was given under the conditions shown in Table 3 from 5 days before the expected delivery date of the mother pig to the delivery date, and the amount of 5′-nucleotide in the mother pig breast milk after delivery and until weaning ( The number of diarrhea piglets and frailly dead piglets were counted for each section (26 days after parturition). In this study, diarrhea and frailty treatment were not performed.
During the test period, the feed used was Winnie Breed (manufactured by Nippon Agricultural Industrial Co., Ltd., mixed feed for breeding pigs), and the administration group was the ribonucleic acid-containing yeast extract obtained in Production Example 1 mixed with the feed. Milking starts immediately after parturition and starts once every day until 1st, 2nd, 3rd, 6th and 3rd day of delivery, then 7th, 14th, 21st and On the 28th day, milking was performed using the finger method.
At the time of milking, 1 ml of a hormonal agent (produced by Teikoku Pharmaceutical Co., Ltd., Atonin) was injected intramuscularly 10 minutes before milking so that milking was easy.
母豚母乳中の５’−ヌクレオチド濃度の推移を図４に示す。
図４より、無投与区に比較して投与区では、母乳中の５’−ヌクレオチド濃度が増加していることが分かる。
表４に、離乳時まで（分娩２６日間）の子豚の飼育成績を、図５に出生子豚の発育曲線を示す。
The transition of the 5′-nucleotide concentration in the sow milk is shown in FIG.
FIG. 4 shows that the 5′-nucleotide concentration in breast milk is increased in the administration group compared to the non-administration group.
Table 4 shows the breeding results of the piglets until weaning (26 days of delivery), and FIG. 5 shows the growth curves of the birth piglets.
表４、図５から明らかなように、無投与区では下痢が多くみられたが、投与区では下痢は見られず、また、出生子豚の発育の良好なものであった。 As is apparent from Table 4 and FIG. 5, diarrhea was observed frequently in the non-administration group, but no diarrhea was observed in the administration group, and the growth of the born piglets was good.
以上説明してきた通り、本発明によれば、母豚に投与するだけという極めて簡単な方法で、母豚母乳中の５’−ヌクレオチド含有量を増加させることができ、子豚の下痢、虚弱等を防止することができるため、成熟度が低く抵抗性の弱い子豚、特に出生から離乳期の子豚、の飼育に、特に好適に用いることができる。 As described above, according to the present invention, it is possible to increase the 5′-nucleotide content in the mother pig's breast milk by an extremely simple method that is simply administered to the mother pig. Therefore, it can be particularly suitably used for breeding piglets with low maturity and weak resistance, particularly those from birth to weaning.
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| JP2006014670A (en) * | 2004-07-01 | 2006-01-19 | Meiji Shiryo Kk | Method for improving meat quality of livestock and/or poultry, and agent for improving meat quality of livestock and/or poultry |
| JP2007008849A (en) * | 2005-06-29 | 2007-01-18 | Meiji Shiryo Kk | Rotavirus infection/proliferation inhibitor |
| WO2008081608A1 (en) | 2006-12-28 | 2008-07-10 | Yukiguni Maitake Co., Ltd. | Method of feeding mother pig |
| US7993668B2 (en) * | 2004-06-28 | 2011-08-09 | Ajinomoto Co., Inc. | Nutrient composition and composition for prevention/mitigation of digestive tract depression |
| CN102987174A (en) * | 2012-12-28 | 2013-03-27 | 北京资源亚太饲料科技有限公司 | Premixed feed for improving lactation yield of sows and preparation method thereof |
| CN102987169A (en) * | 2012-12-31 | 2013-03-27 | 南京同凯兆业生物技术有限责任公司 | Weaned piglet nucleotide feed additive |
| CN108378243A (en) * | 2018-05-17 | 2018-08-10 | 南京同凯兆业生物技术有限责任公司 | A kind of feed for sow additive and its application |
| JPWO2020189746A1 (en) * | 2019-03-19 | 2021-12-23 | 日本製紙株式会社 | Feed additives, pig feed and pig production methods |
| CN114747683A (en) * | 2022-04-25 | 2022-07-15 | 华南农业大学 | Feed for improving birth weight of piglets and reducing stillbirth rate |
-
2004
- 2004-02-25 JP JP2004048851A patent/JP4287763B2/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7993668B2 (en) * | 2004-06-28 | 2011-08-09 | Ajinomoto Co., Inc. | Nutrient composition and composition for prevention/mitigation of digestive tract depression |
| JP2006014670A (en) * | 2004-07-01 | 2006-01-19 | Meiji Shiryo Kk | Method for improving meat quality of livestock and/or poultry, and agent for improving meat quality of livestock and/or poultry |
| JP2007008849A (en) * | 2005-06-29 | 2007-01-18 | Meiji Shiryo Kk | Rotavirus infection/proliferation inhibitor |
| JP4482493B2 (en) * | 2005-06-29 | 2010-06-16 | 明治飼糧株式会社 | Rotavirus infection / proliferation inhibitor |
| WO2008081608A1 (en) | 2006-12-28 | 2008-07-10 | Yukiguni Maitake Co., Ltd. | Method of feeding mother pig |
| CN102987174A (en) * | 2012-12-28 | 2013-03-27 | 北京资源亚太饲料科技有限公司 | Premixed feed for improving lactation yield of sows and preparation method thereof |
| CN102987169A (en) * | 2012-12-31 | 2013-03-27 | 南京同凯兆业生物技术有限责任公司 | Weaned piglet nucleotide feed additive |
| CN108378243A (en) * | 2018-05-17 | 2018-08-10 | 南京同凯兆业生物技术有限责任公司 | A kind of feed for sow additive and its application |
| CN108378243B (en) * | 2018-05-17 | 2021-05-25 | 南京同凯兆业生物技术有限责任公司 | Feed additive for sows and application thereof |
| JPWO2020189746A1 (en) * | 2019-03-19 | 2021-12-23 | 日本製紙株式会社 | Feed additives, pig feed and pig production methods |
| CN114747683A (en) * | 2022-04-25 | 2022-07-15 | 华南农业大学 | Feed for improving birth weight of piglets and reducing stillbirth rate |
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| Publication number | Publication date |
|---|---|
| JP4287763B2 (en) | 2009-07-01 |
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