JP2003113097A - External preparation including aureobasidium culture solution - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an external preparation having physiological function together with improving effect of sense of use. SOLUTION: The external preparation comprises Aureobasidium culture solution or includes the Aureobasidium culture solution.

Description

【発明の詳細な説明】Detailed Description of the Invention

【０００１】[0001]

【発明の属する技術分野】本発明はスキンケア、メーキ
ャップ、ヘアケア、医薬及び医薬外品の用途に用いる外
用剤、更に詳細には、アトピー性皮膚炎の改善、殺菌・
制菌、等の生理的機能性を有すると同時に、しっとり
感、はりつや感、等の使用感向上効果を有する外用剤に
関する。TECHNICAL FIELD The present invention relates to an external preparation for use in skin care, make-up, hair care, medicine and quasi-drugs, and more particularly to improving atopic dermatitis, sterilizing
The present invention relates to an external preparation having physiological functions such as bacteriostatic and the like, and at the same time having an effect of improving feeling of use such as moist feeling, stickiness and the like.

【０００２】[0002]

【従来の技術】各種の生理機能や使用感向上効果が高い
医薬・化成品は従来より存在するが、医師の処方が必要
であったり化学薬品ゆえに副作用が起こる場合があり、
従来より医薬品や化粧品に対する消費者の天然物志向に
は強いものがあった。特に、平成１３年度から化粧品の
全原材料表示が実施されたことで製造・販売側にも天然
物志向が強まってきた。しかしながら、特定単一物質を
工業的に化学合成する医薬・化成品とは異なり、天然物
由来成分には、効率的生産が困難である為に高価である
と共に少量しか供給出来ず、天然物を材料とするので供
給と品質が不安定であり、かつ、効果が低く、さらに
は、色、臭、腐敗、等の理由で原料として使い難いと言
う欠点があった。そこで、低価格での安定供給が可能で
あり、外用剤の品質への悪影響がなく、人体にも安全、
かつ、多機能高効果である天然由来成分と、その成分を
含有する外用剤が切望されている。2. Description of the Related Art Although there are medicines and chemical products that have various physiological functions and a high effect of improving the feeling of use, side effects may occur due to the need for a doctor's prescription or chemicals.
Conventionally, there has been a strong consumer preference for natural products for pharmaceuticals and cosmetics. In particular, since the labeling of all raw materials for cosmetics has been implemented since 2001, the manufacturing and sales side has become more interested in natural products. However, unlike pharmaceuticals and chemical products that chemically synthesize a specific single substance industrially, the ingredients derived from natural products are expensive because they are difficult to produce efficiently, and only small amounts can be supplied. Since it is used as a material, its supply and quality are unstable, its effect is low, and it is difficult to use as a raw material because of its color, odor, decay, etc. Therefore, stable supply at a low price is possible, there is no adverse effect on the quality of the external preparation, it is safe for the human body,
In addition, a naturally derived ingredient that is multifunctional and highly effective, and an external preparation containing the ingredient have been earnestly desired.

【０００３】[0003]

【課題を解決するための手段】美白、皮膚老化防止、皮
膚損傷治癒の効果（特開平１１−３１３６６７）、及
び、免疫賦活作用やコラーゲン類似皮膚保護効果（特願
２０００−３４２３１０）を有するβ−１・３−１・６
−グルカンを主成分とし、さらに、多種多様な微生物代
謝産物を含有するためにβ−１・３−１・６−グルカン
には無い殺菌・制菌作用（特願２０００−３４２３１
０）等をも併せ持つアウレオバシジウム培養液を外用剤
の成分に選び、アウレオバシジウム培養液含有外用剤を
発明した。本発明により、β−１・３−１・６−グルカ
ンやアウレオバシジウム培養液が持つ機能に加え、しっ
とり感、はりつや感、等の使用感をも向上させる外用剤
の提供が可能になった。[Means for Solving the Problems] β- having whitening, prevention of skin aging, healing of skin damage (JP-A-11-313667), and immunostimulating action and collagen-like skin protective effect (Japanese Patent Application No. 2000-342310). 1.3-1.6
-A bactericidal / bactericidal action which β-1 / 3-1.6-glucan does not have because it contains glucan as a main component and further contains various microbial metabolites (Japanese Patent Application No. 2000-34231).
Aureobasidium culture broth having 0) and the like was also selected as a component of the external preparation, and the inventor of the external preparation containing Aureobasidium culture broth was invented. INDUSTRIAL APPLICABILITY According to the present invention, in addition to the functions of β-1,3-1,6-glucan and aureobasidium culture solution, it is possible to provide an external preparation that improves the feeling of use such as moist feeling, stickiness and feeling. became.

【０００４】[0004]

【作用】本発明はアウレオバシジウム培養液として、ア
ウレオバシジウム属（Aureobasidium sp.）に属する微
工研寄託FERM−P４２５７号菌を用いて特開昭５７−１
４９３０１号の方法により製造されたアウレオバシジウ
ム培養液を加熱殺菌したもの、またはアウレオバシジウ
ム属（Aureobasidium sp.）に属する微工研寄託FERM−P
１８０９９号菌を特願２０００−３４２３１０の方法に
より製造したアウレオバシジウム培養液を加熱殺菌した
もの、あるいはβ−１・３−１・６−グルカンを生産す
るアウレオバシジウム属（Aureobasidium sp.）菌株を
用いて上記と同様の方法により製造されたアウレオバシ
ジウム培養液を加熱殺菌したものを用いることにより、
所期の目的を達したものである。The present invention uses, as an aureobasidium culture solution, Microorganism Research Institute deposited FERM-P4257 strain belonging to the genus Aureobasidium sp.
Heat-sterilized aureobasidium culture broth produced by the method of No. 49301, or FERM-P, a deposit of the Institute of Mechanical Engineering, belonging to the genus Aureobasidium sp.
No. 18099 strain produced by heat sterilization of the aureobasidium culture solution produced by the method of Japanese Patent Application No. 2000-342310, or a strain of the genus Aureobasidium (Aureobasidium sp.) That produces β-1 / 3-1.6-glucan. By using a sterilized aureobasidium culture solution produced by the same method as above using
It has achieved its intended purpose.

【０００５】β−１・３−１・６−グルカンを主成分と
するアウレオバシジウム培養液を製造するために使用し
た微生物であるＦＥＲＭ Ｐ−１８０９９の科学的性質
及び分類学の位置と菌学的特徴は次の通りである。 科学的性質及び分類学の位置 （科学的性質）本菌は高粘稠の高分子多糖を産生する。
本物質はエタノールで容易に凝集して簡単に回収でき
る。本多糖はβ型で、主鎖は1，３結合であり、３と６
の位置より分岐をもつ酸性多糖である。有機酸としてリ
ンゴ酸、リン酸などのカルボン酸を含有する。又、アル
ミニウムイオンなどにより容易に凝集する。本物質は飼
料として発育促進や排水処理にも有効であり、免疫を有
する食品添加物、機能性食品として有効である。 （分類学上の位置）ポテトデキストロース寒天斜面培養
上、２５℃、７日間培養で黒褐色のコロニーを形成す
る。コロニー周縁は糸状様発育で次第に淡黒褐色とな
る。細胞は糸状様で、時に分節胞子、酵母様の出芽分生
子，卵形の酵母様単細胞，時として厚膜胞子細胞も形成
される。発育温度２５℃、グルコース、フラクトース、
ガラクトースなどのヘキソース、スクロース、又デンプ
ンを分解する。培養液は顕著な粘稠になる。 菌学的性質から不完全菌類、黒色菌科のAureobasidium
pullulansと近縁。 分離菌の菌学的特徴 コロニーは初め表面平滑で灰白色、粘液性、光沢のある
油滴状（脂肪様）の酵母様に発育し、その周縁から糸状
の菌体が放射状に成長し、ちぢれた様な糸状で丁度樹枝
状発育をする。この糸状菌体は培地表面のみならず培地
中にもよく発育する。しばらくするとコロニー表面に淡
暗褐色の斑点が点々と現われ次第に黒色の斑点になり遂
に全面が暗黒色となる。この糸状菌体に淡褐色の楕円又
は卵形の多数の分生子を側生する。この分生子は容易に
ばらばらになる。一方油滴状のコロニーの表面にも点々
と分生子をつける。糖類を含んだ培養液は非常に粘稠性
となり、液面に厚いコロニーで皮革の黒色培苔を生ず
る。最適発育温度は２０〜２５℃でブドウ糖、シヨ糖な
どの糖類からアルコール類、有機酸類を生成し、又特有
の芳香を有する。 １．培養的特徴 （イ）固体培地：バレイシヨ、グルコース寒天培地上最
初コロニーは表面平滑、透明、光沢ある油滴状、粘稠の
灰白色の酵母様で、コロニーの周縁から放射状にちぢれ
た糸状様の丁度樹枝状の菌体が発育し、この糸状様菌体
は培地表面のみならず、培地中にもよく発育する。やが
て樹枝様のところどころの部分黒褐色になる。培養して
３〜４日たつとコロニー表面に淡暗褐色の斑点が点々と
現れるが、以後次第に淡暗黒色になり全面に広がり、遂
に全体が黒色になる（培養７日）。尚ツアペツク寒天培
地上では発育はおそいが培養的特徴は前記の様である。
コロニー表面が全面黒色になるのに３週間ぐらいかか
る。 （ロ）液体培養：バレイシヨ、グルコース培地中点々と
浮遊状態に菌体が発育し（培養3日）、次第にコロニー
が増え、やがて（培養７日）液中に粘性のコロニーが充
満する。そして管壁に暗褐色の菌苔が現れ、次第に液面
にも出来る（培養１５日）。この菌蓋はゼラチナスな粘
性のある厚いものである。尚ツアペツク培地中にも同様
に発育するが非常におそく菌体も少なく、約３週間で液
面にかなりの黒色菌苔をつくる。 ２．形態的特徴 若い細胞は透明な糸状のちぢれた樹枝状で、菌体（糸状
様）はところどころから黒く卵形の胞子様のものが側生
する。又油滴状のコロニーはその中に点々と黒色の胞子
様のものが着生する。これは衝撃をあたえるとばらばら
になる。 ３．生理的特徴 最適発育温度は２０〜２５℃、グルコース、シユークロ
ースなどから粘性物を生成又グルコースなどの糖類か
ら、アルコール類、有機酸類を生ずる特有の芳香を有す
る。本菌をそのまま培養すると黒色となるが、培養時に
ビタミンＥと乳化剤としてレシチンを含んだ培地に培養
すると無添加にくらべて、淡黒灰白色となり、黒色化を
抑制され、顕著な粘性培養液が得られる。即ち、本菌の
生産培地組成は蔗糖１％、米糖 0.2％、レシチン 0.2
％、ビタミンＥ 0.2％、水100ml、ｐＨ 5.2で、この培
養液を１kg／cm^２の高圧蒸気殺菌して、本菌を接種し、
ジャファーメンターで25℃、0.5ml／min、100rpm、72時
間、通気攪拌培養後、１kg／cm^２、15分間、高圧蒸気殺
菌し、10000rpmで１５分間遠心分離して上澄液を得る。Scientific properties and taxonomic position and taxonomy of FERM P-18099, a microorganism used for producing an aureobasidium culture containing β-1,3-1,6-glucan as a main component. The characteristic features are as follows. Position of scientific properties and taxonomy (scientific properties) This bacterium produces highly viscous polymeric polysaccharides.
This substance is easily aggregated with ethanol and can be easily recovered. This polysaccharide is β-type, the main chain has 1,3 bonds, and 3 and 6
It is an acidic polysaccharide having a branch from the position of. It contains carboxylic acids such as malic acid and phosphoric acid as organic acids. Also, it easily aggregates due to aluminum ions and the like. This substance is effective as a feed for growth promotion and wastewater treatment, and as an immune food additive and a functional food. (Position on taxonomy) A dark brown colony is formed by culturing at 25 ° C. for 7 days on a slope culture of potato dextrose. The periphery of the colony gradually grows light blackish brown with filamentous development. The cells are filamentous, sometimes forming segmental spores, yeast-like budding conidia, oval yeast-like single cells, and sometimes chlamydospore cells. Growth temperature 25 ℃, glucose, fructose,
Decomposes hexoses such as galactose, sucrose, and starch. The culture medium becomes noticeably viscous. Due to mycological properties, Aureobasidium, an incomplete fungus
Closely related to pullulans. Mycological characteristics of the isolated fungus The colony initially grew like a grayish-white, mucous, shiny, oil-drop-like (fat-like) yeast, and filamentous fungi grew radially from the periphery and shredded. Just like dendritic growth. This filamentous fungus grows well not only on the surface of the medium but also in the medium. After a while, spots of light dark brown appeared on the surface of the colony, gradually becoming black spots, and finally the whole surface became dark black. A large number of pale brown elliptical or oval conidia are laterally borne on the filamentous fungus. These conidia easily fall apart. On the other hand, conidia are also sprinkled on the surface of the oily colony. The culture medium containing sugars becomes very viscous, and black colonies of leather are produced with thick colonies on the liquid surface. The optimum growth temperature is 20 to 25 ° C., which produces alcohols and organic acids from sugars such as glucose and sucrose, and has a unique aroma. 1. Cultivation characteristics (a) Solid medium: Valleciyo, glucose on agar medium The first colony is a smooth, transparent, shiny oily, viscous, grayish-white yeast-like, just like filamentous filaments radiating from the periphery of the colony. Dendritic cells grow, and the filamentous cells grow well not only on the surface of the medium but also in the medium. Eventually, some parts of the tree will become dark brown. After 3 to 4 days of culturing, light and dark brown spots appear on the surface of the colony, but thereafter, it gradually becomes light dark black and spreads over the entire surface, and finally the whole becomes black (7 days of culture). The growth is slow on the Tuapetsk agar medium, but the culture characteristics are as described above.
It takes about 3 weeks for the entire colony surface to turn black. (B) Liquid culture: Bacillus and glucose medium, the bacterial cells grow in a floating state in dots (3 days in culture), the number of colonies gradually increases, and eventually (7 days in culture) the solution becomes full of viscous colonies. Then, dark brown fungal moss appeared on the tube wall and gradually formed on the liquid surface (15 days of culture). This fungal lid is thick, gelatinous and viscous. Although it grows similarly in Tuapeck's medium, it has very few bacterial cells, and it forms a considerable amount of black moss on the liquid surface in about 3 weeks. 2. Morphological characteristics Young cells are dendritic, transparent, filamentous, with fungal cells (filamentous) sporadically formed in black and egg-shaped spores in some places. Oily colonies have black spore-like colonies in them. This will fall apart when shocked. 3. Physiological characteristics The optimum growth temperature is 20 to 25 ° C, and it has a unique fragrance that produces viscous substances from glucose, sucrose and the like and alcohols and organic acids from sugars such as glucose. When this fungus is cultivated as it is, it becomes black, but when it is cultivated in a medium containing vitamin E and lecithin as an emulsifier, it becomes a pale blackish-white color compared to when it is not added, blackening is suppressed, and a remarkable viscous culture solution is obtained. To be That is, the composition of the production medium of this bacterium was 1% sucrose, 0.2% rice sugar and 0.2% lecithin.
%, Vitamin E 0.2%, water 100 ml, pH 5.2, this culture broth was sterilized under high pressure steam of 1 kg / cm ² and inoculated with this bacterium,
After culturing with aeration and agitation at 25 ° C., 0.5 ml / min, 100 rpm for 72 hours in a jar fermenter, sterilization under high pressure steam for 1 minute at 1 kg / cm ² and centrifugation for 15 minutes at 10,000 rpm to obtain a supernatant.

【０００６】[0006]

【発明の実施の形態】（用途）本発明の外用剤は、液
体、ゲル状、クリーム状、粉末状、等いろいろな形態を
有することができ、例えば、乳液、クリーム、化粧水、
パック、ジェル、軟膏剤、洗浄料、等のスキンケア化粧
料やマッサージやエステ用外用剤、口紅、ファンデーシ
ョン等のメーキャップ化粧料、ヘアシャンプー、リン
ス、トリートメント、ヘアクリーム、ヘアーパック、ヘ
アーローション、ヘアクリーム、等の頭皮・毛髪用のヘ
アケア用化粧料、石鹸、さらには、軟膏剤、湿布、分散
剤、クリーム剤、外用液剤等の外用の医薬品や医薬部外
品として用いることが出来る。ただし、外用剤としての
用途であれば、これらに限定するものではない。 （含有率）本発明の外用剤におけるアウレオバシジウム
培養液の配合割合は特に限定されないが、外用剤の総重
量に対してアウレオバシジウム培養液が望ましくは０．
１〜１００％、さらに望ましくは５〜１００％含有す
る。詳細には、しっとり感、張りつや感、等の使用感向
上を目的とする場合には５〜２０％、生理機能性を期待
する場合には１０〜１００％が特に望ましい。 （組成）本発明の外用剤は、常法に従い、通常の化粧料
や各種外用剤に使用される種々の形態の基剤に、アウレ
オバシジウム培養液を配合して製造される。通常の化粧
料や各種外用剤に使用される種々の形態の基剤とは、例
えば、油剤、界面活性剤、精製水、アルコール類、高分
子化合物、ゲル化剤、紫外線吸収剤、紫外線散乱防止
剤、酸化防止剤、色素、香料、美容成分、保湿剤、天然
エキス類、医薬品では抗生物質や増量剤、等であり、本
発明の効果を損なわない範囲で、これから選択して用い
ることが出来る。ただし、全体量のうちアルコール含有
率が４０％を超える場合にはアウレオバシジウム培養液
中のβ−１・３−１・６−グルカン、アウレオバシジウ
ム菌の菌体、及び、菌体と培地固形分の複合体が沈殿す
るために、アルコールは外用剤全量の４０％以下で用い
らねばならない。BEST MODE FOR CARRYING OUT THE INVENTION (Use) The external preparation of the present invention can have various forms such as liquid, gel, cream, powder, and the like. For example, emulsion, cream, lotion,
Skin care cosmetics such as packs, gels, ointments, cleaning agents, external preparations for massage and beauty treatments, makeup cosmetics such as lipsticks and foundations, hair shampoos, rinses, treatments, hair creams, hair packs, hair lotions, hair creams. , Etc. can be used as cosmetics for hair care for scalp / hair, soap, and as external medicines and quasi-drugs such as ointments, poultices, dispersants, creams and liquid preparations for external use. However, it is not limited to these as long as it is used as an external preparation. (Content) Although the compounding ratio of the aureobasidium culture solution in the external preparation of the present invention is not particularly limited, the aureobasidium culture solution is preferably 0.
1 to 100%, more preferably 5 to 100%. Specifically, 5 to 20% is particularly desirable for the purpose of improving the feeling of use such as moisturizing feeling, tenseness and the like, and 10 to 100% is particularly desirable for expecting physiological functionality. (Composition) The external preparation of the present invention is produced by mixing an aureobasidium culture solution with various forms of bases used for ordinary cosmetics and various external preparations according to a conventional method. Various forms of bases used for ordinary cosmetics and various external preparations include, for example, oils, surfactants, purified water, alcohols, polymer compounds, gelling agents, UV absorbers, and UV scattering prevention. Agents, antioxidants, pigments, fragrances, beauty ingredients, moisturizers, natural extracts, pharmaceuticals such as antibiotics and extenders, and the like, which can be selected and used within a range that does not impair the effects of the present invention. . However, in the case where the alcohol content rate exceeds 40% of the total amount, β-1,3-1,6-glucan in the aureobasidium culture solution, bacterial cells of aureobasidium, and bacterial cells and medium Alcohol must be used in an amount of 40% or less of the total amount of the external preparation in order to precipitate the solid complex.

【０００７】次に本発明の実施例を詳しく説明するが、
本発明はこれらになんら制約されるものではない。The embodiment of the present invention will be described in detail below.
The present invention is not limited to these.

【実施例】【Example】

【０００８】＜実施例１＞化粧水 本発明の実施例１種類と比較例３種類の化粧水を（表
１）の配合で常法により作成し、使用感を比較評価した
（表２）。評価方法は、２０代、３０代、４０代、５０
代各５人の女性、計２０人をパネラーとし、就寝前に４
種類の被験化粧水を１日交替で顔に塗り、翌朝に肌の張
りつや等の潤い感を評価し、それを記録していった。１
種類につき４回、計１６日この試験を続けた。以下の基
準に従って評価し、その結果を集計した。 ［評価］ ［内容］ 有 効 肌のはり、つやが改善され、潤い感が残っている。 やや有効 肌のはり、つやがやや改善され、若干潤い感が残っている。 無 効 肌のはり、つやに改善が見られず、潤い感を感じない。 表２の結果で明らかなごとく、通常用いられている湿潤
剤や保湿剤を用いた化粧水にくらべ、本発明に基づいて
作成した化粧水の方が潤い感に優れることが明らかとな
った。特に５０代のパネラーの評価では本発明による実
施例に対する評価が高かったことから、肌のはり、つや
を改善する皮膚老化防止効果に優れ、特に高齢者向きの
化粧水に適すると言える。<Example 1> Lotion A lotion of 1 type of the present invention and 3 types of comparative examples of the present invention were prepared by a conventional method with the composition of (Table 1), and the feeling of use was comparatively evaluated (Table 2). Evaluation methods are 20s, 30s, 40s, 50s
5 females each in their teens, 20 panelists in total, 4 before going to bed
Each type of test lotion was applied to the face every day for one day, and the next morning, moisturizing sensations such as skin tension were evaluated and recorded. 1
This test was continued 4 times per type for a total of 16 days. Evaluations were made according to the following criteria, and the results were tabulated. [Evaluation] [Contents] Effectiveness The skin's suppleness and gloss are improved, and a moisturizing feeling remains. Slightly effective The skin's elasticity and gloss are slightly improved, leaving a slightly moisturized feeling. Ineffective Skin, no improvement in gloss, no feeling of moisture. As is clear from the results shown in Table 2, it is clear that the lotion prepared according to the present invention is superior in moisturizing feeling to the lotion containing the moisturizer or the moisturizer which is usually used. In particular, the panelists in their 50s were highly evaluated in the examples according to the present invention, and therefore, they are excellent in the effect of preventing skin aging for improving the skin's suppleness and gloss, and can be said to be particularly suitable for lotions for the elderly.

【０００９】[0009]

【表１】 [Table 1]

【００１０】[0010]

【表２】 [Table 2]

【００１１】＜実施例２＞外用ジェル剤 アウレオバシジウム培養液の原液は高い粘性、付着性、
保湿性を有し、そのままでスキンケア用ジェル、各用途
でのパック剤、塗布用外用剤としての使用が可能であ
る。本実施例ではアウレオバシジウム培養液の原液をそ
のまま外用ジェル剤として用い、２０代、３０代のアト
ピー性皮膚炎の患者１４名をパネラーとして、１日朝、
昼、晩の３回、症状のある領域に外用ジェル剤を塗布
し、これを１０日続けた。以下の基準に従って、１１日
目に評価を行った。ただし、医療行為における評価では
なく、患者の主観的判断に基づく評価である。結果を表
３に記す。 ［評価］ ［内容］ 有 効 痒さ、掻痺感、アトピー症状が改善された。 やや有効 痒さ、掻痺感、アトピー症状がやや改善された。 無 効 痒さ、掻痺感、アトピー症状が改善されなかった。 悪 化 痒さ、掻痺感、アトピー症状が悪化した。<Embodiment 2> A gel solution for external use, Aureobasidium stock solution, has a high viscosity, an adhesive property, and
It has a moisturizing property and can be used as it is as a skin care gel, a pack agent for various purposes, and an external preparation for application. In the present example, the stock solution of aureobasidium culture solution was used as it was as a gel for external use, and 14 patients with atopic dermatitis in their 20s and 30s were panelists, and a day morning,
The gel for external use was applied to the symptomatic area three times a day and evening, and this was continued for 10 days. Evaluation was performed on the 11th day according to the following criteria. However, it is not an evaluation in medical practice but an evaluation based on the subjective judgment of the patient. The results are shown in Table 3. [Evaluation] [Details] Effectiveness Itching, itchiness, and atopic symptoms were improved. Somewhat effective Itching, numbness and atopic symptoms were slightly improved. Ineffectiveness, itching, tingling and atopic symptoms were not improved. Aggravation Itching, numbness and atopic symptoms worsened.

【００１２】[0012]

【表３】 [Table 3]

【００１３】さらに、本試験の実施前日と終了後の１１
日目とに、以下の検査を行い、外用ジェル剤の殺菌或る
いは制菌効果を評価した。すなわち、外用ジェル剤を塗
布する部位を、少量の８０％グリセリンをしみ込ませて
滅菌しておいたガーゼで良く拭き取り、そのガーゼを、
予め滅菌調整しておいた肉汁寒天培地の上に広げて敷
き、３６℃で２４時間培養し、外用ジェル剤塗布の前後
を比較し、皮膚常在菌に対する殺菌或いは制菌効果を下
記の基準によって評価した。結果を表４に記す。 ［評価］ ［内容］ 有 効 微生物のほぼすべてを除去（殺菌）した。 やや有効 微生物を或る程度は減少させた。 無 効 外用ジェル剤塗布の前後で微生物の減少が見られなかった。 悪 化 外用ジェル剤塗布の前よりも微生物が増加した。Furthermore, 11 days before and after the completion of this test
On the day of the test, the following tests were performed to evaluate the sterilization or bacteriostatic effect of the gel for external use. That is, the area to which the gel for external use is applied is wiped well with a gauze that has been sterilized by soaking a small amount of 80% glycerin, and the gauze is
Spread it on the broth agar medium that has been sterilized in advance, incubate at 36 ° C for 24 hours, compare before and after applying the gel for external use, and sterilize or infect the resident bacteria on the skin according to the following criteria. evaluated. The results are shown in Table 4. [Evaluation] [Contents] Almost all effective microorganisms were removed (sterilized). Somewhat effective microbes were reduced. No effect No decrease in microorganisms was observed before and after applying the topical gel. Deterioration Microorganisms increased more than before applying external gel.

【００１４】[0014]

【表４】 [Table 4]

【００１５】アトピー性皮膚炎の症状改善に有効とされ
る医薬品や成分を用いた多くの場合では、数％ないし数
十％は逆に悪化すると言うのが通常であると言われてお
り、本実施例ではパネラーが少数ではあるものの悪化例
が０であったことは評価出来る。皮膚角質層内の微生物
がアトピー性皮膚炎の大きな要因であり、潤いを保つこ
とが症状改善に重要であることは、今日では常識となっ
ている。従って、アウレオバシジウム培養液が保湿性に
優れ免疫賦活能を有していることと、これら２つの試験
結果において殺菌制菌効果とアトピー症状改善効果を示
したことから、本発明は、優れた医薬品としても有用な
外用剤であることが明らかとなった。It is said that, in many cases using a drug or ingredient that is effective for ameliorating the symptoms of atopic dermatitis, it is usually said that a few percent to a few tens percent will worsen. In the example, it can be evaluated that the number of deterioration cases was 0 although the number of panelists was small. It is common knowledge today that microorganisms in the stratum corneum of the skin are a major factor in atopic dermatitis, and that maintaining moisture is important for improving symptoms. Therefore, since the aureobasidium culture solution has excellent moisturizing ability and immunopotentiating ability, and the bactericidal bacteriostatic effect and atopic symptom improving effect were shown in these two test results, the present invention is excellent. It has been clarified that it is a useful external preparation as a pharmaceutical.

【００１６】＜実施例３＞ヘアトニック 表５の配合で常法により作成した、アウレオバシジウム
の培養液を含有するヘアトニックを用いて、フケの発生
防止効果の試験を実施した。<Example 3> Hair Tonic A hair tonic containing a culture solution of aureobasidium prepared by a conventional method with the composition shown in Table 5 was used to test the effect of preventing the occurrence of dandruff.

【００１７】[0017]

【表５】 [Table 5]

【００１８】評価方法は、２０代、３０代、４０代、各
５人の男性で合計１５人をパネラーとし、毎日朝、夕の
２回、２週間にわたって本実施例のヘアトニックを使用
した後に、本実施例を使用する前と比較して、フケ防止
効果を下記の基準に従って評価した。結果を表６に示
す。 ［評価］ ［内容］ 有 効 使用前と比較して、フケの発生がかなり減った。 やや有効 使用前と比較して、フケの発生が少し減った。 無 効 使用前と変化なし。The evaluation method was as follows: 5 men in each of their 20s, 30s, 40s and a total of 15 men were panelists, and after using the hair tonic of this example twice a day every morning and evening for two weeks. The anti-dandruff effect was evaluated according to the following criteria as compared with before using this example. The results are shown in Table 6. [Evaluation] [Contents] Effectively Compared to before use, the occurrence of dandruff was significantly reduced. Slightly effective Compared to before use, the occurrence of dandruff was slightly reduced. Ineffective No change from before use.

【００１９】[0019]

【表６】 [Table 6]

【００２０】本実施例は、頭皮に対する刺激が少なく、
フケの発生を低減するヘアトニックであった。In this embodiment, there is little irritation to the scalp,
It was a hair tonic that reduces the occurrence of dandruff.

【００２１】＜実施例４＞スキンクリーム 本発明の実施例と比較例２種類のスキンクリームを、表
７の配合に基づいて常法により作成した。Example 4 Skin Cream Example of the Present Invention and Comparative Example Two types of skin creams were prepared by a conventional method based on the formulations shown in Table 7.

【００２２】[0022]

【表７】 [Table 7]

【００２３】評価方法、明らかにニキビがみられる１８
〜２６歳の女性３０名を無作為に１０人づつ３のグルー
プに分け、各グループの１０人を、それぞれ、実施例、
比較例１、比較例２を使用するパネラーとした。毎日、
朝と夜の２回、洗顔後に実施例または比較例のスキンク
リームを塗布し、これを２ヵ月続けた。その後、以下の
基準に従って評価した。結果を表８に記す。 ［評価］ ［内容］ 有 効 ニキビの形成が殆ど認められなくなった。 やや有効 ニキビの形成があまり認められなくなった。 無 効 使用前と変化なし。Evaluation Method, Clearly Acne 18
~ 30 women aged 26 to be randomly divided into 3 groups of 10 people, 10 people in each group, respectively, in the example,
A paneler using Comparative Examples 1 and 2 was used. every day,
After washing the face twice in the morning and at night, the skin cream of the Example or Comparative Example was applied, and this was continued for 2 months. Then, it evaluated according to the following criteria. The results are shown in Table 8. [Evaluation] [Details] Effectiveness Acne formation was hardly observed. Somewhat effective formation of acne was not observed. Ineffective No change from before use.

【００２４】[0024]

【表８】 [Table 8]

【００２５】湿潤剤のソルビトール及びトレハロース、
天然の保湿剤で高価なコラーゲン及びコンドロイチン硫
酸、及び、殺菌剤として強力な合成殺菌剤である塩化ベ
ンザルコニウム及び天然殺菌剤のドクダミ抽出物を配合
した比較例１、２に比べ、本実施例の方がニキビ予防効
果に優れ、かつ、肌に優しいスキンクリームであった。Humectants sorbitol and trehalose,
Compared to Comparative Examples 1 and 2 in which collagen and chondroitin sulfate, which are natural moisturizers and are expensive, and benzalkonium chloride, which is a powerful synthetic bactericide as a bactericide, and Dokudami extract of a natural bactericide are mixed, Was a skin cream that is more effective against acne and is gentle on the skin.

【００２６】[0026]

【発明の効果】本発明は、化粧品や医薬品及び高価な天
然成分を用いた外用剤よりも皮膚や頭皮等の各種症状の
改善において優れた効果を有しながら皮膚にも優しく、
従来の化粧品等よりも使用感が向上し、かつ、安価で安
定供給が可能な外用剤の提供を可能とする。本発明によ
り製造した外用剤は、アウレオバシジウム培養液の既知
の主成分であるβ−１・３−１・６−グルカンの有する
免疫賦活効果、皮膚保護効果、皮膚損傷修復効果、美白
効果、皮膚老化防止効果、アウレオバシジウム培養液の
有する殺菌制菌効果を有し、具体的には、肌のはり、つ
や、潤い感の向上、アトピー症状の改善、皮膚常在菌の
抑制、フケやニキビの防止、等の効果を有する外用剤で
あり、特に、今後さらに増加する一方であろう高齢者や
アトピー・アレルギー症状に悩む方の皮膚の保護や状
態、症状の改善にとって、非常に有望有益な外用剤とな
り得ることが明らかとなった。EFFECT OF THE INVENTION The present invention has a superior effect in improving various symptoms such as skin and scalp as compared with external preparations using cosmetics, pharmaceuticals and expensive natural ingredients, and is gentle on the skin,
(EN) It is possible to provide an external preparation that has improved usability compared to conventional cosmetics and is inexpensive and that can be stably supplied. The external preparation produced according to the present invention has an immunostimulating effect, a skin protecting effect, a skin damage repairing effect, a whitening effect, which β-1,3-1,6-glucan, which is a known main component of an aureobasidium culture, has. It has an anti-aging effect on skin, a bactericidal and bactericidal effect that aureobasidium culture has, and specifically, the skin's acuity, gloss, moisturizing sensation, atopic symptom improvement, suppression of skin resident bacteria, dandruff It is an external preparation that has the effect of preventing acne, etc., and it is very promising and beneficial especially for the protection of the skin and the improvement of the condition and symptoms of the elderly and those suffering from atopic / allergic symptoms, which are expected to increase even more in the future. It has become clear that it can be used as an external preparation.

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.⁷ 識別記号 ＦＩ テーマコート゛(参考） Ａ６１Ｋ 7/00 Ａ６１Ｋ 7/00 Ｒ Ｕ 7/02 7/02 7/025 7/025 7/06 7/06 7/075 7/075 7/50 7/50 9/08 9/08 9/10 9/10 9/14 9/14 // Ｃ１２Ｎ 1/14 Ｃ１２Ｎ 1/14 (Ｃ１２Ｎ 1/14 Ｃ１２Ｒ 1:645 Ｃ１２Ｒ 1:645） Ｆターム(参考） 4B065 AA57X AC20 BA22 CA41 CA44 CA50 CA51 CA52 4C076 AA06 AA11 AA17 AA29 BB31 CC03 CC31 4C083 AA031 AA032 AA082 AA112 AC012 AC022 AC092 AC102 AC122 AC132 AC692 AD211 AD212 AD312 AD342 AD432 AD512 CC02 CC04 CC05 CC07 CC11 CC12 CC13 CC23 CC33 CC38 CC39 DD11 DD16 DD17 DD22 DD23 DD27 DD31 DD41 EE10 EE12 EE13 EE14 4C087 AA01 AA02 AA03 BC30 CA10 NA14 ZA89 ZA90 ZB35 ─────────────────────────────────────────────────── ─── Continued Front Page (51) Int.Cl. ⁷ Identification Code FI Theme Coat (Reference) A61K 7/00 A61K 7/00 RU 7/02 7/02 7/025 7/025 7/06 7 / 06 7/075 7/075 7/50 7/50 9/08 9/08 9/10 9/10 9/14 9/14 // C12N 1/14 C12N 1/14 (C12N 1/14 C12R 1: 645 C12R 1: 645) F term (reference) 4B065 AA57X AC20 BA22 CA41 CA44 CA50 CA51 CA52 4C076 AA06 AA11 AA17 AA29 BB31 CC03 CC31 4C083 AA031 AA032 AA082 CC11 AD04 CC512 AD12 CC512 AD05 CC05 AD02 AD12 CC12 CC13 CC23 CC33 CC38 CC39 DD11 DD16 DD17 DD22 DD23 DD27 DD31 DD41 EE10 EE12 EE13 EE14 4C087 AA01 AA02 AA03 BC30 CA10 NA14 ZA89 ZA90 ZB35

Claims (6)

【特許請求の範囲】[Claims] 【請求項１】 アウレオバシジウム培養液を有効成分と
することを特徴とする外用剤。1. An external preparation comprising an aureobasidium culture solution as an active ingredient. 【請求項２】 外用剤の総重量に対し、アウレオバシジ
ウム培養液を０．１〜１００％含有する請求項１記載の
外用剤。2. The external preparation according to claim 1, which contains 0.1 to 100% of the aureobasidium culture solution based on the total weight of the external preparation. 【請求項３】 アウレオバシジウム培養液が、アウレオ
バシジウム属（Aureobasidium sp.）に属する微工研寄
託FERM−P４２５７号菌を用いて特開昭５７−１４９３
０１の方法により製造、或いは、微工研寄託FERM-P１８
０９９号菌を用いて特願２０００−３４２３１０の方法
により製造されたもので、培養液中にβ−１・３−１・
６−グルカン、或いは、リン酸基、リンゴ酸基、硫酸基
等の酸性官能基が結合したβ−１・３−１・６−グルカ
ンを含有するものであること、あるいはβ−１・３−１
・６−グルカン或いはリン酸基やリンゴ酸基等の酸性官
能基を含有するβ−１・３−１・６−グルカンを生産す
るアウレオバシジウム属の菌株を用いて製造されたもの
である請求項１または２記載の外用剤。3. A method for producing an aureobasidium culture broth, using the micro-machine research deposit FERM-P4257 strain belonging to the genus Aureobasidium sp.
Manufactured by the method of No. 01, or deposited by MICROFERM-P18
It was produced by the method of Japanese Patent Application No. 2000-342310 using Bacteria No. 099, and β-1.3-1.
It contains 6-glucan or β-1,3-1,6-glucan to which an acidic functional group such as a phosphoric acid group, a malic acid group or a sulfuric acid group is bound, or β-1 / 3- 1
· A product produced using a strain of the genus Aureobasidium which produces β-3-1-3,6-glucan containing 6-glucan or an acidic functional group such as a phosphate group or a malate group. Item 1. The external preparation according to Item 1 or 2. 【請求項４】 アウレオバシジウム培養液が、加熱殺菌
した液状のものまたはこの加熱殺菌の後さらに遠心や濾
過によって浮遊物を除去した液状のもの、あるいはそれ
らを凍結乾燥や噴霧乾燥等の手段により粉末化したもの
である請求項１から３までのいずれか１項記載の外用
剤。4. An aureobasidium culture broth is a liquid sterilized by heat sterilization, a liquid liquor obtained by removing suspended matter by centrifugation or filtration after the heat sterilization, or by freeze-drying or spray-drying them. The external preparation according to any one of claims 1 to 3, which is a powder. 【請求項５】 外用剤が、液体、ゲル状、クリーム状、
粉末状、その他いろいろな形態を有することができ、乳
液、クリーム、化粧水、パック、ジェル、軟膏剤、洗浄
料、等のスキンケア化粧料やマッサージやエステ用外用
剤、口紅、ファンデーション等のメーキャップ化粧料、
ヘアシャンプー、リンス、トリートメント、ヘアクリー
ム、ヘアーパック、ヘアーローション、ヘアクリーム、
等の頭皮・毛髪用のヘアケア用化粧料、石鹸、さらに
は、軟膏剤、湿布、分散剤、クリーム剤、外用液剤等の
外用の医薬品や医薬部外品、その他外用剤としての用途
を有する外用剤を含有している請求項１から４までのい
ずれか１項記載の外用剤。5. The external preparation may be liquid, gel, cream,
It can be in powder form and various other forms. Skin care cosmetics such as emulsions, creams, lotions, packs, gels, ointments, cleaning agents, and external makeup for massage and beauty treatments, lipsticks, makeup makeup such as foundations. Fee,
Hair shampoo, conditioner, treatment, hair cream, hair pack, hair lotion, hair cream,
Cosmetics for hair care such as scalp and hair, soap, and external medicines and quasi-drugs such as ointments, poultices, dispersants, creams and liquids for external use, and other external applications having applications as external preparations. The external preparation according to any one of claims 1 to 4, which contains an agent. 【請求項６】 外用剤が、常法に従い、通常の化粧料や
各種外用剤に使用される種々の形態の基剤に、アウレオ
バシジウム培養液を配合して製造されたものである請求
項１から５までのいずれか１項記載の外用剤。6. The external preparation is produced according to a conventional method by mixing an aureobasidium culture solution with various forms of bases used for ordinary cosmetics and various external preparations. The external preparation according to any one of 1 to 5.