JP2001518191A - 無担体偏向電気泳動法 - Google Patents
無担体偏向電気泳動法Info
- Publication number
- JP2001518191A JP2001518191A JP54486998A JP54486998A JP2001518191A JP 2001518191 A JP2001518191 A JP 2001518191A JP 54486998 A JP54486998 A JP 54486998A JP 54486998 A JP54486998 A JP 54486998A JP 2001518191 A JP2001518191 A JP 2001518191A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- separation
- electrodes
- electrophoresis
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrostatic Separation (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.担体としての分離媒体および分析する試料が入口端から出口端まで分離チャ ンバ(1)を通り流れ、前記試料は、電極(6、7)により発生させる電界により空 間的に画分に分離され収集される無担体偏向電気泳動法であって、前記試料を、 前記電極(6、7)に電圧を印加していない状態で前記電極(6、7)間にある分離 スペースに導入し、前記試料を前記分離スペースに導入した後前記電極(6、7) に電圧を印加し、前記電極(6、7)間の分離スペースに、実質的に試料全体が依 然存在している間のみ電気泳動分離を実施することを特徴とする無担体偏向電気 泳動法。 2.前記試料が前記分離媒体とともに静止している状態で前記電気泳動分離を実 施することを特徴とする請求項1に記載の方法。 3.前記試料の導入中の流量に対して、前記入口端から出口端までの前記電極( 6、7)間のゆっくりとした流れまで低下させた分離媒体および試料の流量で電 気泳動分離を実施することを特徴とする請求項1に記載の方法。 4.試料の成分または標識物質が、前記分離チャンバ(1)内の一定空間位置に達 したことを外部検出器が確認したらすぐに前記電気泳動分離法を終了することを 特徴とする前記請求項のいずれかに記載の方法。 5.分離された試料の成分が、電界を印加していない状態で、高流量で分別装置 を介して分離された試料用容器に運搬されることを特徴とする請求項1に記載の 方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19711898A DE19711898A1 (de) | 1997-03-21 | 1997-03-21 | Verfahren und Vorrichtung zur trägerfreien Ablenkungs-Elektrophorese im Intervall-Betrieb |
DE19711898.4 | 1997-03-21 | ||
PCT/EP1998/001648 WO1998043077A1 (de) | 1997-03-21 | 1998-03-20 | Verfahren zur trägerfreien ablenkungselektrophorese |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2001518191A true JP2001518191A (ja) | 2001-10-09 |
JP4137186B2 JP4137186B2 (ja) | 2008-08-20 |
Family
ID=7824182
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54486998A Expired - Lifetime JP4137186B2 (ja) | 1997-03-21 | 1998-03-20 | 無担体偏向電気泳動法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US6328868B1 (ja) |
EP (1) | EP0968417B1 (ja) |
JP (1) | JP4137186B2 (ja) |
DE (2) | DE19711898A1 (ja) |
WO (1) | WO1998043077A1 (ja) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008539443A (ja) * | 2005-04-29 | 2008-11-13 | ベクトン・ディキンソン・アンド・カンパニー | 並行かつ同時分離を伴う電気泳動方法 |
JP2009505119A (ja) * | 2005-04-29 | 2009-02-05 | ベクトン・ディキンソン・アンド・カンパニー | 特性の異なる媒体を使用する電気泳動のための方法 |
JP2010508496A (ja) * | 2006-08-29 | 2010-03-18 | ベクトン・ディキンソン・アンド・カンパニー | 無担体の偏向電気泳動の方法及び装置 |
JP2010060350A (ja) * | 2008-09-02 | 2010-03-18 | Yokogawa Electric Corp | ガス分析計 |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10047088C2 (de) * | 2000-09-21 | 2002-10-17 | Gerhard Weber | Medium für analytische und präparative Elektrophorese |
DE10063096C1 (de) * | 2000-12-18 | 2002-09-12 | Gerhard Weber | Elektrophoresevorrichtung, Elektrophoreseverfahren unter Verwendung einer Elektrophoresevorrichtung und Verwendung der Elektrophoresevorrichtung |
DE10063097B4 (de) * | 2000-12-18 | 2007-04-19 | Becton, Dickinson And Co. | Elektrophoresevorrichtung |
JP2004518663A (ja) * | 2000-12-18 | 2004-06-24 | トラスティーズ オブ プリンストン ユニバーシティ | 非対称なパルスフィールド電気泳動を使用した巨大分子の分画 |
US7597791B2 (en) * | 2001-10-19 | 2009-10-06 | The Trustees Of Princeton University | Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules |
US20040137526A1 (en) * | 2002-10-15 | 2004-07-15 | The Regents Of The University Of Michigan | Multidimensional protein separation system |
US20080237044A1 (en) | 2007-03-28 | 2008-10-02 | The Charles Stark Draper Laboratory, Inc. | Method and apparatus for concentrating molecules |
WO2008130618A1 (en) * | 2007-04-19 | 2008-10-30 | The Charles Stark Draper Laboratory, Inc. | Method and apparatus for separating particles, cells, molecules and particulates |
AU2008265103B2 (en) * | 2007-06-20 | 2014-05-22 | Becton, Dickinson And Company | FFE media and FFE methods comprising volatile separation media |
US7837379B2 (en) * | 2007-08-13 | 2010-11-23 | The Charles Stark Draper Laboratory, Inc. | Devices for producing a continuously flowing concentration gradient in laminar flow |
AU2008322937A1 (en) * | 2007-11-12 | 2009-05-22 | Becton, Dickinson & Company | MS-compatible nonionic or zwitterionic surfactants in free-flow electrophoresis |
WO2009133152A1 (en) * | 2008-04-29 | 2009-11-05 | Becton, Dickinson And Company | Method and system for measuring a sample to determine the presence of and optionally treat a pathologic condition |
CA2856779C (en) | 2011-11-22 | 2021-07-20 | Stephen G. Haralampu | Stopped-flow, m icro-flu i imc device and method for the charge-based separation of complex analyte mixtures |
EP4012395A1 (en) | 2020-12-11 | 2022-06-15 | Gerhard Weber | Free-flow electrophoresis method for separating analytes |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3847773A (en) | 1973-06-11 | 1974-11-12 | Technicon Instr | Method and apparatus for curtain electrophoresis |
DE4139472C1 (ja) * | 1991-11-29 | 1993-03-11 | Gerhard Dr. 8011 Kirchheim De Weber |
-
1997
- 1997-03-21 DE DE19711898A patent/DE19711898A1/de not_active Withdrawn
-
1998
- 1998-03-20 EP EP98917035A patent/EP0968417B1/de not_active Expired - Lifetime
- 1998-03-20 JP JP54486998A patent/JP4137186B2/ja not_active Expired - Lifetime
- 1998-03-20 DE DE59812413T patent/DE59812413D1/de not_active Expired - Lifetime
- 1998-03-20 US US09/380,621 patent/US6328868B1/en not_active Expired - Lifetime
- 1998-03-20 WO PCT/EP1998/001648 patent/WO1998043077A1/de active IP Right Grant
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008539443A (ja) * | 2005-04-29 | 2008-11-13 | ベクトン・ディキンソン・アンド・カンパニー | 並行かつ同時分離を伴う電気泳動方法 |
JP2009505119A (ja) * | 2005-04-29 | 2009-02-05 | ベクトン・ディキンソン・アンド・カンパニー | 特性の異なる媒体を使用する電気泳動のための方法 |
JP4860693B2 (ja) * | 2005-04-29 | 2012-01-25 | ベクトン・ディキンソン・アンド・カンパニー | 並行かつ同時分離を伴う電気泳動方法 |
JP2010508496A (ja) * | 2006-08-29 | 2010-03-18 | ベクトン・ディキンソン・アンド・カンパニー | 無担体の偏向電気泳動の方法及び装置 |
JP2010060350A (ja) * | 2008-09-02 | 2010-03-18 | Yokogawa Electric Corp | ガス分析計 |
Also Published As
Publication number | Publication date |
---|---|
US6328868B1 (en) | 2001-12-11 |
EP0968417A1 (de) | 2000-01-05 |
WO1998043077A1 (de) | 1998-10-01 |
DE59812413D1 (de) | 2005-01-27 |
EP0968417B1 (de) | 2004-12-22 |
JP4137186B2 (ja) | 2008-08-20 |
DE19711898A1 (de) | 1998-09-24 |
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