JP2001500744A - 細胞を保持する方法及び装置 - Google Patents
細胞を保持する方法及び装置Info
- Publication number
- JP2001500744A JP2001500744A JP10521601A JP52160198A JP2001500744A JP 2001500744 A JP2001500744 A JP 2001500744A JP 10521601 A JP10521601 A JP 10521601A JP 52160198 A JP52160198 A JP 52160198A JP 2001500744 A JP2001500744 A JP 2001500744A
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.細胞を保持するための装置であって、 動的制御された環境を有し、所望条件に維持された環境内で細胞を生長させ 、動的制御され所望条件に維持された環境内で細胞が検査される細胞培養機構と 、 前記細胞培養機構に連繋され、細胞の状態を決定する機構を具えている、細 胞保持装置。 2.培養機構は、バイオチャンバーを有するハウジングを含んでいる請求項1に 記載の装置。 3.培養機構は、細胞の増殖が行われる第1の凹所と少なくとも第2の凹所を有 しており、第1及び第2の凹所は、ハウジングのバイオチャンバーの中に配備さ れる請求項2に記載の装置。 4.ハウジングは、バイオチャンバーの中の第1及び第2の凹所を観察すること ができる第1のポート機構を具えており、決定機構は、第1のポート機構に隣接 して配備されて第1及び第2の凹所内の細胞の像を作成する画像機構を含んでい る請求項3に記載の装置。 5.ハウジングは、バイオチャンバーへ流体を伝達する第2のポート機構を具え ており、培養機構は、バイオチャンバー内の環境を制御する機構を含んでおり、 該環境制御機構は第2のポート機構に連繋されている請求項4に記載の装置。 6.環境制御機構は、第1及び第2の凹所内の細胞を所定温度に維持するための バイオチャンバーに熱を伝達する加熱機構を含んでいる請求項5に記載の装置。 7.画像機構は、第1の凹所又は第2の凹所内の細胞が増加したかどうかを確認 するためのコンピュータを具えており、該コンピュータは、画像機構から第1及 び第2の凹所の像を受けるために画像機構に接続されている請求項6に記載の装 置。 8.画像機構は、第1及び第2の凹所を観察する顕微鏡機構を具えており、該顕 微鏡機構は第1ポートに隣接して配備され、コンピュータに連繋されている請求 項7に記載の装置。 9.画像機構は、第1及び第2の凹所内の像を作成するカメラ機構を具えており 、該カメラ機構は第1及び第2の凹所内の細胞の顕微鏡機構による像を撮影でき るように顕微鏡機構に接続され、該カメラ機構はコンピュータに接続されている 請求項8に記載の装置。 10.決定機構は、顕微鏡機構が第1及び第2の凹所内の細胞を観察できるよう に、第1及び第2の凹所を顕微鏡機構に関して移動させる移動機構を含んでいる 請求項9に記載の装置。 11.第1のポート機構は、ハウジングの頂部に配備された第1の窓と、ハウジ ングの底部に配備された第2の窓を含んでおり、第1の窓と第2の窓の間を光が 通 過する光学経路を形成するために、第2の窓は第1の窓と光学的に同一線上にあ る請求項10に記載の装置。 12.培養機構は、透明プレートを具えており、該透明プレート内に第1及び第 2の凹所が形成される請求項11に記載の装置。 13.環境制御機構は、バイオチャンバーに連繋されて第1及び第2の凹所内の 培地のpHを調節する機構と、バイオチャンバーに連繋されて圧力を調節する機 構を具えている請求項12に記載の装置。 14.培養機構は、培地を第1又は第2の凹所へ自動的に分配し、第1又は第2 の凹所から自動的に吸引するロボット機構を含んでおり、該ロボット機構はバイ オチャンバーに隣接し、第1及び第2の凹所と移動可能に接触している請求項1 3に記載の装置。 15.ロボット機構は、第1及び第2の凹所に関する新鮮培地及び廃棄培地のた めの貯蔵機構を含んでいる請求項14に記載の装置。 16.決定機構は、ロボット機構に連繋されて第1又は第2の凹所内で予め定め られた事象の発生を確認するための診断機構を含んでいる請求項15に記載の装 置。 17.決定機構は、顕微鏡機構に接続されて第1及び第2の凹所に関する顕微鏡 機構の位置を制御するための操作レバーを含んでいる請求項16に記載の装置。 18.移動機構は、第1又は第2の凹所の位置が顕微鏡 機構と同一線上になるようにプレートを移動させる移動システムを含んでいる請 求項17に記載の装置。 19.ロボット機構は、zロボットピペットを含んでおり、該ピペットを通じて 、培地は第1若しくは第2の凹所から吸引され、又は第1若しくは第2の凹所へ 分配される請求項18に記載の装置。 20.画像機構は、第1又は第2の凹所内の細胞の像作成を向上させるために、 zロボットピペットにより第1又は第2の凹所へ導入される蛍光物質を含んでい る請求項19に記載の装置。 21.ロボット機構は、zロボットピペット及び貯蔵器機構に接続されて液体を 貯蔵器機構からzロボット付機構へ移すための注入器ポンプを具えている請求項 20に記載の装置。 22.注入器ポンプ機構は液体を移すための力を供給するステッパモータを具え ている請求項21に記載の装置。 23.貯蔵器機構は増殖培地を有する第1の貯蔵器と、休止培地を有する少なく とも第2の貯蔵器を含んでいる請求項22に記載の装置。 24.貯蔵器機構は、注入器ポンプ機構、zロボットピペット、第1の貯蔵器及 び第2の貯蔵器に接続されてどの貯蔵器からどの液体をzロボットピペットへ移 すかを指示するための分配弁機構を含んでいる請求項2 3に記載の装置。 25.zロボットピペットは、ピペットの先端周囲に存在する流体を検知し、先 端周囲に流体が存在することを示すピペット信号を発生するセンサー機構を有し ている請求項24に記載の装置。 26.ピペット信号を受信するコンピュータを含んでおり、該コンピュータは、 ステッパモータに接続されてステッパモータの動作を制御する請求項25に記載 の装置。 27.ハウジングの隔壁には、zロボットピペットがバイオチャンバーの中へ進 入するための孔が開設されている請求項26に記載の装置。 28.分配弁機構は、診断機構に接続され、第1又は第2の凹所から培地を受け 取る請求項27に記載の装置。 29.分配弁機構は、該分配弁機構を制御するコンピュータに接続される請求項 28に記載の装置。 30.診断機構は、培地を分析する検定手段を含んでいる請求項29に記載の装 置。 31.検定手段はカラムを含んでいる請求項30に記載の装置。 32.検定手段はクロマトグラフを含んでいる請求項31に記載の装置。 33.細胞を処理する装置であって、 バイオチャンバーと、該バイオチャンバーの中に配 備されて細胞が入れられる少なくとも第1の凹所を有するハウジングと; バイオチャンバーに接触し、第1の凹所へ培地を自動的に分配し、第1の凹 所から培地を自動的に吸引するロボット機構を具えている、細胞の処理装置。 34.チャンバー内の細胞を保持する方法であって、 第1の細胞を走査するステップ; 第1の細胞が***した時を決定するステップを有している、細胞の保持方法 。 35.走査ステップの前に、増殖培地を第1の細胞と共に配置するステップがあ る請求項34に記載の方法。 36.走査ステップの前に、第1の細胞の位置を確認するステップがある請求項 35に記載の方法。 37.決定ステップの後、増殖培地を休止培地と取り替えるステップがある請求 項36に記載の方法。 38.走査ステップの前に、増殖培地を第2の細胞と共に配置し、第2の細胞の 位置を確認するステップがある請求項37に記載の方法。 39.走査ステップの後に、第2の細胞を走査するステップがある請求項38に 記載の方法。 40.取り替えるステップは、第1の細胞の周囲から増殖培地を吸引する位置へ zロボットピペットを移動させるステップ;第1の細胞の周囲から増殖培地を吸 引するステップ;第1の細胞の周囲に休止培地を加える ステップを含んでいる請求項39に記載の方法。 41.幹細胞を使用するための方法であって、 幹細胞を得るステップ; 幹細胞を増殖させるために、自動化されたシステムの中へ幹細胞を配置する ステップ; 自動化されたシステムの中で、自動化されたシステムに入れられた幹細胞か ら追加の幹細胞を生長させるステップ; 自動化されたシステムの中で幹細胞を集めるステップを有している、幹細胞 の使用方法。 42.幹細胞を集めるステップの後、幹細胞を患者に移植するステップがある請 求項41に記載の方法。 43.幹細胞を得るステップは、幹細胞を患者から取り除くステップがある請求 項42に記載の方法。 44.幹細胞を生長させるステップは、分化された成熟血液細胞の生長を最少に しつつ、多分化能幹細胞の生長を最大ならしめるステップを含んでいる請求項4 3に記載の方法。 45.幹細胞を生産する方法であって、 インサイチューにて、第1の幹細胞と共に増殖培地を配置するステップ; インサイチューにて、第1の幹細胞が第2及び第3の幹細胞へ***する時を 決定するステップ; インサイチューにおける第2及び第3の幹細胞の周 りの増殖培地を、インサイチューにて第2及び第3の幹細胞の委任プロジェニタ ーへの分化を抑える休止培地と交換するステップを有している、幹細胞の生産方 法。 46.細胞を保持するための方法であって、 動的制御され、所望条件に維持された環境内で細胞が検査され、該環境内で 細胞を培養するステップ; 細胞の状態を決定するステップを有している、細胞の保持方法。
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WO2011004568A1 (ja) * | 2009-07-08 | 2011-01-13 | 株式会社ニコン | 受精卵観察の画像処理方法、画像処理プログラム及び画像処理装置、並びに受精卵の製造方法 |
JP2013520198A (ja) * | 2010-02-26 | 2013-06-06 | マサチューセッツ インスティテュート オブ テクノロジー | 脊椎生物幼体(larvae)を対象としたサブ細胞分解能生体内(インビーボ)スクリーニング用の高スループットプラットフォーム |
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US7867752B1 (en) | 2011-01-11 |
JP2008092957A (ja) | 2008-04-24 |
CA2239815A1 (en) | 1998-05-14 |
JP4203108B2 (ja) | 2008-12-24 |
US6008010A (en) | 1999-12-28 |
JP4099236B2 (ja) | 2008-06-11 |
DE69740113D1 (de) | 2011-03-17 |
EP0877792A4 (en) | 2006-04-12 |
US8859263B2 (en) | 2014-10-14 |
CA2239815C (en) | 2011-10-25 |
AU5159298A (en) | 1998-05-29 |
WO1998020108A1 (en) | 1998-05-14 |
US8445261B2 (en) | 2013-05-21 |
EP0877792A1 (en) | 1998-11-18 |
US20020155487A1 (en) | 2002-10-24 |
EP0877792B1 (en) | 2011-02-02 |
AU742909B2 (en) | 2002-01-17 |
US8241892B2 (en) | 2012-08-14 |
US20130023041A1 (en) | 2013-01-24 |
US20140371112A1 (en) | 2014-12-18 |
US20110195055A1 (en) | 2011-08-11 |
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