JP2000509412A - 疎水性の立体配座制限的微細環境を提供する部分によって強化された重合フルオロホア - Google Patents
疎水性の立体配座制限的微細環境を提供する部分によって強化された重合フルオロホアInfo
- Publication number
- JP2000509412A JP2000509412A JP9527793A JP52779397A JP2000509412A JP 2000509412 A JP2000509412 A JP 2000509412A JP 9527793 A JP9527793 A JP 9527793A JP 52779397 A JP52779397 A JP 52779397A JP 2000509412 A JP2000509412 A JP 2000509412A
- Authority
- JP
- Japan
- Prior art keywords
- dye
- polymer
- anilino
- group
- cyclodextrin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 235000005074 zinc chloride Nutrition 0.000 description 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B69/00—Dyes not provided for by a single group of this subclass
- C09B69/10—Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/141111—Diverse hetero atoms in same or different rings [e.g., alkaloids, opiates, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/17—Nitrogen containing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/18—Sulfur containing
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Materials For Medical Uses (AREA)
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. (a)基幹ポリマーと、 (b)前記基幹ポリマーに共有結合した複数個の信号発生基とから成り、 前記信号発生基が、複素環に少なくとも1つの窒素原子を含む複素環部分にエ チレン不飽和連結基によって結合された少なくとも1つのアニリノ部分を有する 染料に由来することを特徴とするポリマー染料。 2. 前記基幹ポリマーが求核性であり、前記信号発生基が求電子性であること を特徴とする請求項1に記載のポリマー染料。 3. 前記信号発生基が、式: A−L−B 〔式中、Aは複素環基を表し、 Lは連結基を表し、 Bはアニリノ基を表す〕 によって表されることを特徴とする請求項1に記載のポリマー染料。 4. Bが、式: を有することを特徴とする請求項3に記載のポリマー染料。 5. Lが、式: 〔式中、nは1、2または3を示す〕を有することを特徴とする請求項3に記載 のポリマー染料。 6. 前記信号発生基がカルボキシル基を含むことを特徴とする請求項2に記載 のポリマー染料。 7. 前記信号発生基が、アミノピリジニウムアニリノ、アミノキノリニウムア ニリノ、及び、アミノアクリジニウムアニリノから成るグループから選択された 染料に由来することを特徴とする請求項6に記載のポリマー染料。 8. 前記信号発生基が、カルボキシキノリニウムアニリノ、カルボキシベンザ チアゾリウムアニリノ、カルボキシベンズオキサゾリウムアニリノ、カルボキシ アクリジニウムアニリノ、 カルボキシベンズイミダゾリウムアニリノ、カルボキシナフトインドリウムアニ リノ、カルボキシナフタチアゾリウムアニリノ、カルボキシナフトイミジゾリウ ムアニリノ、及び、カルボキシナフトオキサゾリウムアニリノから成るグループ から選択された染料に由来することを特徴とする請求項6に記載のポリマー染料 。 9. 前記信号発生基が構造式: 〔式中、R1はアルキル基を表し、R2はアルキル基を表し、nは1〜3(両端値 を含む)の整数を表す〕を有する染料に由来することを特徴とする請求項6に記 載のポリマー染料。 10.前記信号発生基が構造式: 〔式中、R1はアルキル基を表し、R2はアルキル基を表し、nは1〜3(両端値 を含む)の整数を表し、X-は負電荷の対イオンを表す〕を有する染料に由来す ることを特徴とする請求項6に記載のポリマー染料。 11. 前記基幹ポリマーが、当該基幹ポリマーに共有結合した複数の疎水性の 立体配座制限部分を有することを特徴とする請求項2に記載のポリマー染料。 12. 前記疎水性の立体配座制限部分が、シクロデキストリン、カルセランド 、カリキシラン、分子クレフト、ククルビテリル及びシクロファンから成るグル ープから選択された部分であることを特徴とする請求項11に記載のポリマー染 料。 13. 請求項2に記載のポリマー染料と、前記ポリマー染料に会合しているが 前記基幹ポリマーに共有結合していない複数の疎水性の立体配座制限部分とから 成る組成物。 14. 前記疎水性の立体配座制限部分が、シクロデキストリン、カルセランド 、カリキシラン、分子クレフト、ククルビテリル及びシクロファンから成るグル ープから選択された部分であることを特徴とする請求項13に記載の組成物。 15. 前記基幹ポリマーが求電子性であり、前記信号発生基 が求核性であることを特徴とする請求項1に記載のポリマー染料。 16. 前記基幹ポリマーが、当該基幹ポリマーに共有結合した複数の疎水性の 立体配座制限部分を有することを特徴とする請求項15に記載のポリマー染料。 17. 前記疎水性の立体配座制限部分が、シクロデキストリン、カルセランド 、カリキシラン、分子クレフト、ククルビテリル及びシクロファンから成るグル ープから選択された部分であることを特徴とする請求項16に記載のポリマー染 料。 18. 請求項15に記載のポリマー染料と、前記ポリマー染料に会合している が前記基幹ポリマーに共有結合していない複数の疎水性の立体配座制限部分とか ら成る組成物。 19. 前記疎水性の立体配座制限部分が、シクロデキストリン、カルセランド 、カリキシラン、分子クレフト、ククルビテリル及びシクロファンから成るグル ープから選択された部分であることを特徴とする請求項18に記載の組成物。 20. 結合対の一方の因子と、 前記因子に結合した少なくとも1つのポリマー染料と、 前記ポリマー染料に会合した疎水性の立体配座制限部分とから 成るコンジュゲート。 21. 前記ポリマー染料に会合した前記疎水性の立体配座制限部分が前記少な くとも1つのポリマー染料に共有結合していないことを特徴とする請求項20に 記載のコンジュゲート。 22. 前記ポリマー染料に会合した前記疎水性の立体配座制限部分が前記少な くとも1つのポリマー染料に共有結合していることを特徴とする請求項20に記 載のコンジュゲート。 23. 前記ポリマー染料に会合した前記疎水性の立体配座制限部分が、シクロ デキストリン、カルセランド、カリキシラン、分子クレフト、ククルビテリル及 びシクロファンから成るグループから選択された部分であることを特徴とする請 求項20に記載のコンジュゲート。 24. 前記少なくとも1つのポリマー染料が、最適化された高蛍光性ポリマー であることを特徴とする請求項20に記載のコンジュゲート。 25. 前記ポリマー染料が、アミノピリジニウムアニリノ、アミノキノリニウ ムアニリノ、及び、アミノアクリジニウムアニリノから成るグループから選択さ れた染料に由来することを特徴とする請求項20に記載のコンジュゲート。 26. 前記ポリマー染料が、カルボキシキノリニウムアニリノ、カルボキシベ ンザチアゾリウムアニリノ、カルボキシベンズオキサゾリウムアニリノ、カルボ キシアクリジニウムアニリノ、カルボキシベンズイミダゾリウムアニリノ、カル ボキシナフトインドリウムアニリノ、カルボキシナフタチアゾリウムアニリノ、 カルボキシナフトイミジゾリウムアニリノ、及び、カルボキシナフトオキサゾリ ウムアニリノから成るグループから選択された染料に由来することを特徴とする 請求項20に記載のコンジュゲート。 27. 前記ポリマー染料が、式:〔式中、R1はアルキル基を表し、R2はアルキル基を表し、nは1〜3(両端値 を含む)の整数を表す〕のベンゾチアゾールピリジニウムトリエンから成ること を特徴とする請求項20に記載のコンジュゲート。 28. 前記ポリマー染料が、式: 〔式中、R1はアルキル基を表し、R2はアルキル基を表し、nは1〜3(両端値 を含む)の整数を表し、X-は負電荷の対イオンを表す〕のアミノスチリルビリ ジニウムから成ることを特徴とする請求項20に記載のコンジュゲート。 29. 前記ポリマー染料に会合した前記疎水性の立体配座制限部分が、前記ポ リマー染料に共有結合したβ−シクロデキストリンアルデヒド部分であることを 特徴とする請求項20に記載のコンジュゲート。 30. a.試験サンプルを準備し、 b.前記試験サンプルから細胞を単離し、 c.前記試験サンプルに溶解誘発剤を導入し、 d.前記試験サンプルに請求項20に記載のコンジュゲートを添加し、 e.前記試験サンプルの蛍光を測定する、 段階から成る細胞表現形質のイムノタイピング方法。
Applications Claiming Priority (3)
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US08/595,092 US5994143A (en) | 1996-02-01 | 1996-02-01 | Polymeric fluorophores enhanced by moieties providing a hydrophobic and conformationally restrictive microenvironment |
US08/595,092 | 1996-02-01 | ||
PCT/US1997/001429 WO1997028447A1 (en) | 1996-02-01 | 1997-01-30 | Polymeric fluorophores enhanced by moieties providing a hydrophobic and conformationally restrictive microenvironment |
Publications (3)
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JP2000509412A true JP2000509412A (ja) | 2000-07-25 |
JP2000509412A5 JP2000509412A5 (ja) | 2004-11-11 |
JP4255514B2 JP4255514B2 (ja) | 2009-04-15 |
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JP52779397A Expired - Fee Related JP4255514B2 (ja) | 1996-02-01 | 1997-01-30 | 疎水性の立体配座制限的微細環境を提供する部分によって強化された重合フルオロホア |
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US (1) | US5994143A (ja) |
EP (1) | EP1019722B1 (ja) |
JP (1) | JP4255514B2 (ja) |
AT (1) | ATE237138T1 (ja) |
DE (1) | DE69720809T2 (ja) |
ES (1) | ES2197332T3 (ja) |
WO (1) | WO1997028447A1 (ja) |
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1996
- 1996-02-01 US US08/595,092 patent/US5994143A/en not_active Expired - Lifetime
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1997
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- 1997-01-30 DE DE69720809T patent/DE69720809T2/de not_active Expired - Fee Related
- 1997-01-30 WO PCT/US1997/001429 patent/WO1997028447A1/en active IP Right Grant
- 1997-01-30 EP EP97904060A patent/EP1019722B1/en not_active Expired - Lifetime
- 1997-01-30 ES ES97904060T patent/ES2197332T3/es not_active Expired - Lifetime
- 1997-01-30 JP JP52779397A patent/JP4255514B2/ja not_active Expired - Fee Related
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JP2013040136A (ja) * | 2011-08-17 | 2013-02-28 | Tosoh Corp | 4−アルコキシ−2−(4−アミノスチリル)ベンゾチアゾリウム塩、その製造方法およびそれを用いる核酸の検出方法 |
JP2019522066A (ja) * | 2016-05-10 | 2019-08-08 | ソニー株式会社 | ポリマー染料およびシクロデキストリンを含む組成物、ならびにその使用 |
JP7068192B2 (ja) | 2016-05-10 | 2022-05-16 | ソニーグループ株式会社 | ポリマー染料およびシクロデキストリンを含む組成物、ならびにその使用 |
US11390754B2 (en) | 2016-05-10 | 2022-07-19 | Sony Corporation | Compositions comprising a polymeric dye and a cyclodextrin and uses thereof |
JP2019522688A (ja) * | 2016-05-11 | 2019-08-15 | ソニー株式会社 | 超明色ダイマーまたはポリマー染料 |
JP7071286B2 (ja) | 2016-05-11 | 2022-05-18 | ソニーグループ株式会社 | 超明色ダイマーまたはポリマー染料 |
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WO1997028447A1 (en) | 1997-08-07 |
EP1019722A1 (en) | 2000-07-19 |
DE69720809T2 (de) | 2004-04-01 |
JP4255514B2 (ja) | 2009-04-15 |
US5994143A (en) | 1999-11-30 |
ATE237138T1 (de) | 2003-04-15 |
ES2197332T3 (es) | 2004-01-01 |
EP1019722B1 (en) | 2003-04-09 |
DE69720809D1 (de) | 2003-05-15 |
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