JP2000505290A - シロイヌナズナgai遺伝子をコードする核酸 - Google Patents
シロイヌナズナgai遺伝子をコードする核酸Info
- Publication number
- JP2000505290A JP2000505290A JP9528314A JP52831497A JP2000505290A JP 2000505290 A JP2000505290 A JP 2000505290A JP 9528314 A JP9528314 A JP 9528314A JP 52831497 A JP52831497 A JP 52831497A JP 2000505290 A JP2000505290 A JP 2000505290A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- plant
- sequence
- gai
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.第4図に示すアミノ酸配列を含むポリペプチドをコードするヌクレオチド 配列を含む核酸単離物。 2.コード・ヌクレオチド配列が第3図に示すコード・ヌクレオチド配列を含 む、請求項1記載の核酸。 3.第3図に示すコード・ヌクレオチド配列の1つ以上のヌクレオチドの追加 、置換、挿入および/または欠失により、コード・ヌクレオチド配列が突然変異 体、対立遺伝子、誘導体または形質転換体を含む、請求項1記載の核酸。 4.第4図に示す品種シロイヌナズナのGAIアミノ酸配列の突然変異体、対 立遺伝子、誘導体または形質転換体の配列であるアミノ酸配列を含むポリペプチ ドをコードするヌクレオチド配列を有する核酸単離物、あるいは別の品種の相同 体またはその突然変異体、対立遺伝子、誘導体または形質転換体であって、前記 突然変異体、対立遺伝子、誘導体、形質転換体または相同体の1つ以上のアミノ 酸が第4図のアミノ酸配列と挿入、欠失、追加および/または置換によって異な り、植物中での前記核酸の発現が該植物の成長の阻害をもたらし、該阻害がジベ レリン(GA)によって拮抗される核酸単離物。 5.植物中での前記核酸の過剰発現が該植物に矮性表現型を付与し、その矮性 表現型がGA処理によって変更可能である、請求項4記載の核酸。 6.前記ポリペプチドが第4図に下線で示した17個のアミノ酸配列を含む、 請求の範囲第4項または第5項による核酸。 7.第4図に下線で示した17個のアミノ酸配列中の残基と対応する位置で少 なくとも10個の残基が類似性を有する17個のアミ ノ酸残基の近接配列を前記ポリペプチドが含む、請求項4または5記載の核酸。 8.第4図に示す品種シロイヌナズナのGAIアミノ酸配列の突然変異体、対 立遺伝子、誘導体または形質転換体の配列であるアミノ酸配列を含むポリペプチ ドをコードするヌクレオチド配列を有する核酸単離物、あるいは別の品種の相同 体またはその突然変異体、対立遺伝子、誘導体または形質転換体であって、前記 突然変異体、対立遺伝子、誘導体、形質転換体または相同体の1つ以上のアミノ 酸が第4図のアミノ酸配列と挿入、欠失、追加および/または置換によって異な り、植物中での前記核酸の発現が該植物にGAI非発現突然変異体表現型を補足 し、このような表現型がパクロブトラゾールの矮性効果に耐性である核酸単離物 。 9.前記植物がシロイヌナズナである、請求項4〜8のいずれかに記載の核酸 。 10.第4図に下線で示した17個のアミノ酸配列あるいは第4図に下線で示 した17個のアミノ酸配列中の残基と対応する位置で少なくとも10個の残基が 類似性を有する17個の近接アミノ酸配列の欠失を除いて請求項8記載の核酸に よってコードされるアミノ酸配列を含むポリペプチドをコードするヌクレオチド 配列を有する核酸単離物。 11.1つ以上のアミノ酸の挿入、欠失、追加および/または置換によって、 第4図に示す品種シロイヌナズナのGAIアミノ酸配列あるいは別の品種の相同 体の突然変異体、対立遺伝子、誘導体または形質転換体の配列であるアミノ酸配 列を含むポリペプチドをコードするヌクレオチド配列を有する核酸単離物であっ て、植物中での前記核酸の発現が該植物にジベレリン非応答性の表現型を付与す る核酸単離物。 12.ポリペプチドが、第4図に下線で示した17個のアミノ酸が欠失した第 4図に示すアミノ酸配列を含む、請求項11記載の核酸。 13.コード・ヌクレオチド配列が、第3図に示すコード・ヌクレオチド配列 であるが第4図に下線で示したアミノ酸をコードするヌクレオチドが欠失した配 列を含む、請求項12記載の核酸。 14.コード・ヌクレオチド配列が、1つ以上のアミノ酸の挿入、欠失、追加 および/または置換によって、第3図に示すコード・ヌクレオチド配列であるが 第4図に下線で示したアミノ酸をコードするヌクレオチドが欠失した配列の突然 変異体、対立遺伝子、誘導体または形質転換体の配列であるアミノ酸配列を含む 、請求項12記載の核酸。 15.第4図に下線で示した17個のアミノ酸配列の欠失、および1つ以上の アミノ酸の挿入、欠失、追加および/または置換によって、ポリペプチドが第4 図に示すアミノ酸配列の突然変異体、対立遺伝子、誘導体または形質転換体の配 列であるアミノ酸配列を有する、請求項11記載の核酸。 16.前記植物がシロイヌナズナである、請求項15〜11のいずれかに記載 の核酸。 17.1つ以上のアミノ酸の挿入、欠失、追加および/または置換によって、 第4図に示す品種シロイヌナズナのGAIアミノ酸配列の突然変異体、対立遺伝 子、誘導体または形質転換体の配列であるアミノ酸配列を含むポリペプチドをコ ードするヌクレオチド配列を有する核酸であって、該ポリペプチドが第6b図、 第6d図、第6f図または第6h図に示すアミノ酸配列を有する核酸。 18.前記コード・ヌクレオチド配列が、第6a図、第6c図、第6e図また は第6g図に示す配列である、請求項17記載の核酸 。 19.前記コード・ヌクレオチド配列からの発現の調節配列をさらに含む、請 求項1〜18のいずれかに記載の核酸。 20.前記調節配列が誘導可能なプロモーターを含む、請求項19記載の核酸 。 21.前記コード配列の発現のアンチセンスまたはセンス調節(「コサプレッ ション」)での使用に適した、コード配列の少なくとも14個の近接ヌクレオチ ドの配列と相補的なヌクレオチド配列または請求項1〜15のいずれかに記載の 核酸のコード配列に相補的な配列を有する核酸単離物。 22.DNAであって、前記相補的なヌクレオチド配列がアンチセンス転写の 調節配列の調節下にある、請求項21記載の核酸。 23.前記調節配列が誘導可能なプロモーターを含む、請求項22記載の核酸 。 24.植物細胞の形質転換に適し、請求項1〜23のいずれかに記載の核酸を 含む核酸ベクター。 25.請求項1〜23のいずれかに記載の異種性核酸を含む宿主細胞。 26.微生物である請求項25記載の宿主細胞。 27.植物細胞である請求項25記載の宿主細胞。 28.非相同の前記核酸をそのゲノム内に有する請求項27記載の植物細胞。 29.半数体ゲノムにつき1つを超える前記ヌクレオチド配列を有する請求項 28記載の植物細胞。 30.植物、植物の一部または植物胎芽、あるいは植物の抽出物または派生物 に含まれる、請求項27〜29のいずれかに記載の植物細胞。 31.請求項25〜30のいずれかに記載の細胞を産生する方法であって、前 記核酸を形質転換によって細胞へ取り込む段階を含む方法。 32.核酸が安定してそこに組込まれるようにその核酸を細胞ゲノム核酸と組 換える段階を含む、請求項31記載の方法。 33.1個以上の形質転換細胞からの植物の再生を含む、請求項31または3 2記載の方法。 34.請求項27〜29のいずれかに記載の植物細胞を含む植物。 35.有性または無性で繁殖させた子、請求項31記載の植物のクローンまた は子孫、あるいは前記の植物、子、クローンまたは子孫の任意の一部または胎芽 である植物。 36.請求項35記載の植物の一部または胎芽、あるいは抽出物または派生物 。 37.植物を作出する方法であって、請求項1〜24のいずれかに記載の核酸 を植物細胞に組み込む段階と、前記植物細胞から植物を再生する段階を含む方法 。 38.前記植物細胞から再生した植物の子または子孫を有性または無性で繁殖 させるか成長させる段階を含む、請求項37記載の方法。 39.植物の特徴に影響を及ぼす方法であって、植物の細胞内で請求項1〜3 のいずれかに記載の異種性核酸からの発現を引き起こすか可能にする段階を含む 方法。 40.植物の特徴に影響を及ぼす方法であって、植物の細胞内で請求項4〜7 のいずれかに記載の異種性核酸からの発現を引き起こすか可能にする段階を含む 方法。 41.植物の特徴に影響を及ぼす方法であって、植物の細胞内で 請求項8または9記載の異種性核酸からの発現を引き起こすか可能にする段階を 含む方法。 42.植物の特徴に影響を及ぼす方法であって、植物の細胞内で請求項10〜 16のいずれかに記載の異種性核酸からの発現を引き起こすか可能にする段階を 含む方法。 43.植物の特徴に影響を及ぼす方法であって、植物の細胞内で請求項21〜 23のいずれかに記載の異種性核酸からの発現を引き起こすか可能にする段階を 含む方法。 44.請求項1〜3のいずれかに記載の核酸のトランスジェニック植物の作出 での使用。 45.請求項4〜7のいずれかに記載の核酸のトランスジェニック植物の作出 での使用。 46.請求項8または9記載の核酸のトランスジェニック植物の作出での使用 。 47.請求項10〜16のいずれかに記載の核酸のトランスジェニック植物の 作出での使用。 48.請求項21〜23のいずれかに記載の核酸のトランスジェニック植物の 作出での使用。
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GBGB9602796.6A GB9602796D0 (en) | 1996-02-12 | 1996-02-12 | Genetic control of plant growth and development |
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Publication number | Priority date | Publication date | Assignee | Title |
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GB9314261D0 (en) | 1993-07-09 | 1993-08-18 | Zeneca Ltd | Dwarf plants |
GB9602796D0 (en) * | 1996-02-12 | 1996-04-10 | Innes John Centre Innov Ltd | Genetic control of plant growth and development |
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- 1997-02-12 AU AU17996/97A patent/AU723363B2/en not_active Ceased
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- 2004-06-17 US US10/868,829 patent/US20050039227A1/en not_active Abandoned
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012518406A (ja) * | 2009-02-23 | 2012-08-16 | ポステック アカデミー‐インダストリー ファウンデーション | 開花遅延または成長抑制機能を有するポリペプチド、これを暗号化するポリヌクレオチドおよびこれらの用途 |
WO2013146171A1 (ja) * | 2012-03-29 | 2013-10-03 | 国立大学法人鳥取大学 | イネ科植物を短稈化させる遺伝子および短稈イネ科植物の作出方法 |
US10351872B2 (en) | 2012-03-29 | 2019-07-16 | National University Corporation Shizuoka University | Gene for shortening culm of gramineous plant and method for producing short-culmed gramineous plant |
Also Published As
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AU1799697A (en) | 1997-08-28 |
ATE440106T1 (de) | 2009-09-15 |
US6307126B1 (en) | 2001-10-23 |
CA2244229C (en) | 2007-10-30 |
CA2244229A1 (en) | 1997-08-14 |
CN100506986C (zh) | 2009-07-01 |
WO1997029123A3 (en) | 1997-10-09 |
US6830930B2 (en) | 2004-12-14 |
US20030084470A1 (en) | 2003-05-01 |
WO1997029123A2 (en) | 1997-08-14 |
CN1228784A (zh) | 1999-09-15 |
US20020049995A1 (en) | 2002-04-25 |
US6794560B2 (en) | 2004-09-21 |
JP4068145B2 (ja) | 2008-03-26 |
DE69739539D1 (de) | 2009-10-01 |
GB9602796D0 (en) | 1996-04-10 |
US20050071897A1 (en) | 2005-03-31 |
EP0904290A2 (en) | 1999-03-31 |
US20050039227A1 (en) | 2005-02-17 |
EP0904290B1 (en) | 2009-08-19 |
AU723363B2 (en) | 2000-08-24 |
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