JP2000217582A - Gene coding for enzyme group participating in biosynthesis of icosapentaenoic acid and/or docosahexaenoic acid - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new gene derived from a chromosomal DNA in docosahexaenoic acid productive bacteria, is contained in a gene having a specific restriction map and codes for enzyme groups participating in the biosynthesis of icosapentaenoic acid or docosahexaenoic acid. SOLUTION: This new gene obtained by treating chromosomal DNA in docosahexaenoic acid productive bacteria [e.g. Schewanella sp. SCRC-21406 (FERM BP-5979) or the like] with restriction enzyme San 3AI is contained in a gene fragment of about 37 kb having a restriction map represented by the formula and codes for enzyme groups participating in the biosynthesis of icosapentaenoic acid and/or docosahexaenoic acid, and is used in the profitable method or the like for producing icosapentaenoic acid (EPA) and/or docosahexaenoic acid(DHA) useful as a raw material for medicines, foods, feeds or the like. This gene is obtained by producing a genomic DNA library from a genomic DNA in Schewanella sp. SCRC-21406 and screening out this library with a probe comprising of the partial sequence.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【０００１】[0001]

【発明の属する技術分野】本発明は、医薬、食品、飼料
などの原料として有用なイコサペンタエン酸（以下ＥＰ
Ａと称する）及び／又はドコサヘキサエン酸（以下ＤＨ
Ａと称する）の生合成に関与する酵素群をコードする遺
伝子、遺伝子を含有するプラスミド、該プラスミドによ
り形質転換された微生物に関するものである。The present invention relates to icosapentaenoic acid (hereinafter referred to as EP) useful as a raw material for medicines, foods, feeds and the like.
A) and / or docosahexaenoic acid (hereinafter DH)
A), a gene encoding an enzyme group involved in the biosynthesis of the gene, a plasmid containing the gene, and a microorganism transformed with the plasmid.

【０００２】[0002]

【従来の技術】従来から、微生物の働きにより、ＤＨＡ
を製造する試みがなされている。例えば、海洋性微細藻
類｛Phytochemistry, 30, 2963-2967 (1991), 特開平7-
8221,特開平7-95875｝や鞭毛菌類｛J. Am. Oil Chem.
Soc., 68, 509-514 (1991),特開平1-199588｝を用いた
ＤＨＡ含有トリグリセリドの製造法の報告がある。ま
た、高圧条件下あるいは5℃以下の低温下における深海
性微生物によるＤＨＡの産生に関する報告｛Appl. Envi
ron. Microbiol., 51, 730-737 (1986)（ビブリオ・マ
リナスATCC15381）; Lipids, 29, 527-528 (1994)｝、
海洋細菌を用いたＤＨＡ及びＤＨＡ含有リン脂質の製造
法の報告（特開平10-191993）がある。しかしながら、
これらの微生物のＤＨＡ生合成に関与する遺伝子につい
ては報告されていない。2. Description of the Related Art DHA has been conventionally used by the action of microorganisms.
Attempts have been made to produce For example, marine microalgae Phytochemistry, 30, 2963-2967 (1991), JP-A-7-
8221, JP-A-7-95875 and flagellated fungi J. Am. Oil Chem.
Soc., 68, 509-514 (1991), a method for producing a DHA-containing triglyceride using JP-A-1-199588｝. In addition, a report on the production of DHA by deep-sea microorganisms under high pressure conditions or at low temperatures of 5 ° C or less {Appl. Envi
ron. Microbiol., 51, 730-737 (1986) (Vibrio marinas ATCC 15381); Lipids, 29, 527-528 (1994)｝,
There is a report on a method for producing DHA and DHA-containing phospholipids using marine bacteria (JP-A-10-191993). However,
Genes involved in DHA biosynthesis of these microorganisms have not been reported.

【０００３】一方、ＤＨＡと同様の高度不飽和脂肪酸で
あるＥＰＡを生産する海洋細菌の染色体ＤＮＡ中より、
ＥＰＡ生合成系遺伝子群が取得されており、これを導入
した組み換え大腸菌を用いたＥＰＡの製造法が報告され
ている〔特開平6-46864号{WO93/23545-A (EPO594868-
A)}、特開平8-242867号、WO98/01565〕。しかしなが
ら、これらの報告にはＤＨＡの生産に関する記述はな
い。On the other hand, from the chromosomal DNA of marine bacteria that produce EPA, which is a highly unsaturated fatty acid similar to DHA,
An EPA biosynthesis gene group has been obtained, and a method for producing EPA using recombinant Escherichia coli transfected with the gene has been reported [JP-A-6-46864 {WO93 / 23545-A (EPO594868-
A)}, JP-A-8-242867, WO98 / 01565]. However, these reports do not describe DHA production.

【０００４】[0004]

【発明が解決しようとする課題】有用な物質を生産する
野生株の該有用物質の生産性は一般に低く、それらの微
生物の能力を工業的に利用しようとする場合、種々の方
法により微生物の改良、すなわち生産性の向上が行われ
る。本発明は、遺伝子組み換え法を用いて、文献未知の
ＤＨＡ生合成酵素群遺伝子を見出し、他の生物体に導入
することにより、ＤＨＡ生産性の向上を目指すと共に、
ＤＨＡ生合成能を持たない生物へのＤＨＡ生合成能の賦
与を目的とし、ひいてはＤＨＡの有利な製造法を確立し
ようとするものである。また同時に、ＥＰＡのより効率
的な生産に使用可能な新たな遺伝子群を見出すことをも
目的とする。The productivity of a useful substance in a wild strain which produces a useful substance is generally low, and when the ability of those microorganisms is to be used industrially, the improvement of the microorganism by various methods is required. That is, productivity is improved. The present invention aims to improve DHA productivity by finding a DHA biosynthetic enzyme group gene unknown in the literature using a gene recombination method and introducing it into other organisms.
An object of the present invention is to provide a DHA biosynthesis ability to an organism having no DHA biosynthesis ability, thereby establishing an advantageous method for producing DHA. At the same time, another object is to find a new gene group that can be used for more efficient production of EPA.

【０００５】[0005]

【課題を解決するための手段】本発明者らは鋭意研究の
結果、ＤＨＡ産生海洋細菌シーワネラsp. SCRC-21406株
(FERM BP-5979)の染色体DNA中より、ＥＰＡ生合成系遺
伝子群の欠失変異体のＥＰＡ生産能を回復させる能力を
有する遺伝子群を見出した。さらにこれをＥＰＡ生合成
系遺伝子群と組み合わせることによってＤＨＡの生産が
可能であることを見出し、本発明を完成した。従って本
発明は、ＥＰＡ及び／又はＤＨＡの生合成酵素群遺伝
子、それを含有する発現型プラスミド、該プラスミドに
より形質転換された微生物を提供する。Means for Solving the Problems The present inventors have conducted intensive studies and have found that a DHA-producing marine bacterium Shiwanella sp. SCRC-21406 strain is used.
From the chromosomal DNA of (FERM BP-5979), a gene group having the ability to restore the EPA production ability of a deletion mutant of the EPA biosynthesis gene group was found. Furthermore, they found that DHA can be produced by combining this with the EPA biosynthesis gene group, and completed the present invention. Accordingly, the present invention provides EPA and / or DHA biosynthetic enzyme genes, expression plasmids containing the genes, and microorganisms transformed with the plasmids.

【０００６】[0006]

【発明の実施の形態】本発明においては、ＤＨＡを生産
する能力を有する微生物（遺伝子源微生物）からDNAを
抽出し、このDNAを制限酵素で切断することによってＥ
ＰＡ及び／又はＤＨＡ生合成に関与する酵素群をコード
する遺伝子を切り出し、これを適当なベクターに導入す
ることによって発現型プラスミドを作成する。DESCRIPTION OF THE PREFERRED EMBODIMENTS In the present invention, DNA is extracted from a microorganism (gene source microorganism) having the ability to produce DHA, and the DNA is cut with a restriction enzyme to produce EHA.
A gene encoding a group of enzymes involved in PA and / or DHA biosynthesis is cut out and introduced into an appropriate vector to prepare an expression plasmid.

【０００７】本発明においては、ＤＨＡ生合成酵素群の
遺伝子源の例として、シーワネラsp. SCRC-21406株(FER
M BP-5979)を用いる場合について具体的に説明している
が、ＤＨＡを生産することができるものであればいずれ
でもよく、特に属、種あるいは株などを限定するもので
はない。これらの微生物については公的微生物寄託機関
で容易に入手でき、また自然界から新たに分離すること
もできる。[0007] In the present invention, as an example of the gene source of the DHA biosynthetic enzyme group, Shiwanella sp. SCRC-21406 strain (FER
MBP-5979) is specifically described, but any method can be used as long as it can produce DHA, and the genus, species, strain, and the like are not particularly limited. These microorganisms are readily available from public microbial depositaries and can be newly isolated from nature.

【０００８】本発明の遺伝子を用いることにより、例え
ばエシェリヒャ・コリ(Escherichiacoli)や枯草菌(Baci
llus subtilis)のごとき異種宿主もしくはシーワネラ属
細菌のごとき同種宿主である細菌、あ るいはさらに酵
母、糸状菌等を形質転換することにより、ＥＰＡやＤＨ
Ａ生産株を人為的に創製することができる。さらに、大
豆、ひまわり、アブラナ、ごま等の高等植物に本遺伝子
を導入することにより、ＥＰＡやＤＨＡ産生植物を作り
出すことも可能である。ＥＰＡ及び／又はＤＨＡ生合成
酵素群遺伝子の発現のための領域としては、これらの酵
素の遺伝子に本来付随している制御領域を使用すること
も可能であるが、発現量を向上させ、あるいは発現の誘
導を可能にするためには別途プロモーター／オペレータ
ー系を用意するのが好ましい。宿主として大腸菌を用い
る場合には、このようなプロモーター／オペレーター系
として、T7, lac, bla, trp、tac、lavUV5、P_L、P_R、l_P
P等のプ ロモーター／オペレーター系を用いることがで
き、ＳＤ配列として、trpリーダ ーペプチド、lacZ、メ
タピロカテカ−ゼまたはcII遺伝子等のＳＤ配列を用い
る ことができる。さらにコード領域の下流に転写ター
ミネーター、例えば、大腸菌のリボゾーム遺伝子のrrnB
T₁T₂ターミネーター等を設けることができる。さらに、
前記遺伝子の発現においては、サッカロミセス・セレビ
シエ(Saccharomyces cerevisiae)の宿主／ベクター系を
用いることができ、この場合のプロモーターとしては酵
母のアルコール脱水素酵素遺伝子のプロモーター、酸性
ホスファターゼ遺伝子のプロモーター、グリセルアルデ
ヒド-3-リン酸脱水素酵素のプロモータ ー、エノラーゼ
遺伝子のプロモーター等を使用することができ、この場
合のプラスミドは酵母中で複製するための配列、及び該
プラスミドを含有する酵母を選択するための選択マーカ
ーとしての栄養要求性マーカー、例えばLeu、Trp、His
等 の要求性の配列を含有することが好ましい。[0008] By using the gene of the present invention, for example, Escherichiacoli or Bacillus subtilis (Baci
By transforming a heterologous host such as llus subtilis) or a homologous host such as a bacterium belonging to the genus Shiwanella, or a yeast or a filamentous fungus, EPA or DH can be obtained.
A production strain can be artificially created. Furthermore, by introducing the present gene into higher plants such as soybean, sunflower, rape and sesame, it is also possible to produce EPA and DHA-producing plants. As a region for expression of EPA and / or DHA biosynthetic enzyme group genes, control regions originally associated with the genes of these enzymes can be used. It is preferable to separately prepare a promoter / operator system in order to enable the induction of the promoter. When Escherichia coli is used as a host, T7, lac, bla, trp, tac, lavUV5, P _L , P _R , I _P
A promoter / operator system such as _P can be used, and an SD sequence such as trp leader peptide, lacZ, metapyrocatecase, or cII gene can be used as the SD sequence. Further downstream of the coding region is a transcription terminator, for example, rrnB of the ribosome gene of E. coli.
A T ₁ T ₂ terminator or the like can be provided. further,
For expression of the gene, a host / vector system of Saccharomyces cerevisiae can be used, and in this case, a promoter of a yeast alcohol dehydrogenase gene, a promoter of an acid phosphatase gene, a glyceraldehyde -3-Phosphate dehydrogenase promoter, enolase gene promoter, and the like can be used. In this case, the plasmid is a sequence for replication in yeast and a plasmid for selecting yeast containing the plasmid. An auxotrophic marker as a selectable marker such as Leu, Trp, His
It is preferable to contain a required sequence such as

【０００９】高等植物への遺伝子導入においては、ベク
ターを用いる方法や直接導入法などがある。ベクターと
してはTiプラスミドや、カリフラワーモザイクウイルス
(CaMV)、ジェミニウイルス、カサバラテントウイルス、
トマトゴールデンモザイクウイルスなどのＤＮＡウイル
ス、ブロムモザイクウイルス(BMV)、タバコモザイクウ
イルス(TMV)などのＲＮＡウイルスを用いることがで
き、この場合のプロモータ ーとしてCaMVの35Ｓプロモ
ーターなどを挙げることができる。また、プロトプラス
トへの直接導入法としてリン酸カルシウム法、ポリエチ
レングリコール法、マイクロインジェクション、エレク
トロポレーション、リポソーム法などが挙げられる。ま
た、植物細胞への直接導入法としてパーティクルガン法
も挙げられる。[0009] Gene transfer into higher plants includes a method using a vector and a direct transfer method. Vectors include Ti plasmid and cauliflower mosaic virus
(CaMV), Gemini virus, Casa Barent's tent virus,
DNA viruses such as tomato golden mosaic virus, brom mosaic virus (BMV), tobacco mosaic virus
An RNA virus such as virus (TMV) can be used. In this case, a promoter such as the 35S promoter of CaMV can be used. Examples of the method of direct introduction into protoplasts include a calcium phosphate method, a polyethylene glycol method, microinjection, electroporation, and a liposome method. Further, as a method for direct introduction into a plant cell, a particle gun method may be used.

【００１０】なお、一般に大腸菌における特定の蛋白質
の発現量は遺伝子のコピ−数、転写効率、ｍＲＮＡの安
定性、翻訳効率、蛋白質の安定性等に影響される。遺伝
子工学的技術を用いてこれらの制御領域であるプロモー
ター、ＳＤ領域、ターミネーター等を改変するためには
プラスミドは小型である方が取り扱いが容易である。ま
た、遺伝子のコピー数は、その遺伝子をコードしている
プラスミドの大きさに関連し、小型である方がコピー数
は増加する傾向がある。このため、本発明の実施例で述
べるＥＰＡ及び／又はＤＨＡの生合成に関与する酵素群
遺伝子を含有するDNA断片をプラスミドに挿入し、この
プラスミドDNAのサブクローニングを反復することによ
って該遺伝子断片中の不要部分を切除することにより、
一層小型のプラスミドを得ることも可能である。この方
法によって得られる、より小型化したＥＰＡ及び／又は
ＤＨＡ生合成酵素群遺伝子も本発明に含まれる。また、
酵素の安定性や活性を高めるために、公知の手法を用い
て、遺伝子の一部塩基配列（アミノ酸配列）を変換する
ことも可能であり、あるいは遺伝子の複製の段階におい
て数塩基の挿入、欠失または置換が起こる可能性もある
が、これらの変異遺伝子も本発明に含まれる。なお、本
発明において、ＥＰＡ及び／又はＤＨＡの生合成に関与
する酵素群をコードする遺伝子は、配列番号２，４，６
及び８で示される塩基配列によってコードされるものと
ストリンジェントな条件でハイブリダイズするものを包
含する。In general, the expression level of a specific protein in Escherichia coli is affected by the copy number of a gene, transcription efficiency, mRNA stability, translation efficiency, protein stability, and the like. In order to modify these control regions such as promoter, SD region, terminator and the like using genetic engineering techniques, the smaller the plasmid, the easier it is to handle. The copy number of a gene is related to the size of a plasmid encoding the gene, and the smaller the size, the more the copy number tends to be. For this purpose, a DNA fragment containing a gene group of enzymes involved in the biosynthesis of EPA and / or DHA described in Examples of the present invention is inserted into a plasmid, and subcloning of this plasmid DNA is repeated, whereby the gene fragment in the gene fragment is obtained. By cutting off unnecessary parts,
It is also possible to obtain smaller plasmids. The downsized EPA and / or DHA biosynthetic enzyme genes obtained by this method are also included in the present invention. Also,
In order to enhance the stability and activity of the enzyme, it is possible to convert a partial base sequence (amino acid sequence) of the gene using a known method, or to insert or delete several bases at the stage of gene replication. Loss or substitution may occur, but these mutant genes are also included in the present invention. In the present invention, the genes encoding the enzymes involved in the biosynthesis of EPA and / or DHA are represented by SEQ ID NOs: 2, 4, 6
And those that hybridize under stringent conditions with those encoded by the nucleotide sequence represented by 8.

【００１１】本発明の宿主大腸菌としては、エシェリヒ
ャ・コリK12株に由来する任意の菌株を使用することが
できる。例えば、JM83、JM101、JM103、JM105、JM109、
JM109(DE3)、RR1 、RB791、W3110、C600、HB101、DH1、
AG1、NM554、BL21(DE3)等を使用することができる。ま
た宿主酵母としては、AH22、DC5、D-13-1A、YNN140等が
挙げられる。As the host Escherichia coli of the present invention, any strain derived from Escherichia coli K12 strain can be used. For example, JM83, JM101, JM103, JM105, JM109,
JM109 (DE3), RR1, RB791, W3110, C600, HB101, DH1,
AG1, NM554, BL21 (DE3) and the like can be used. Examples of the host yeast include AH22, DC5, D-13-1A, and YNN140.

【００１２】なお、本発明の遺伝子がコードする酵素群
は、宿主生物体が本来有する生合成系により合成される
高級脂肪酸をＥＰＡ及び／又はＤＨＡに誘導できる。さ
らには、本遺伝子群の一部を用いて宿主生物体の脂肪酸
組成を変革することも可能である。The enzymes encoded by the gene of the present invention can induce higher fatty acids synthesized by the biosynthesis system inherently possessed by the host organism into EPA and / or DHA. Furthermore, it is also possible to modify the fatty acid composition of the host organism using a part of this gene group.

【００１３】次に、本発明を実施例によりさらに具体的
に説明する。Next, the present invention will be described more specifically with reference to examples.

【００１４】[0014]

【実施例】【Example】

【００１５】実施例１−１． シーワネラsp.SCRC-2140
6のゲノムDNAの調製 ペプトン1.0%、酵母エキス0.5%を1/2濃度の人工海水に
溶解し、pH7.0に調整した培地100mlを121℃、20分間加
熱滅菌した後、シーワネラsp.SCRC-21406(FERM BP-597
9)を接種し、15℃で24時間好気的に培養した。培養後、
遠心分離機で菌体を採取し、20mlのSET緩衝液(50mM EDT
A, 20%Sucrose, 50mM Tris-HCl, pH7.6)で一回洗浄し
た。得られた菌体をドライアイス／アセトン中で２分間
凍結し、水中で溶解したのち、2mlの同緩衝液に懸濁し
た。ここに1mgのリゾチームと1mgのRNase Aを添加し、
混合したのち氷中で15分間放置した。さらに10%SDSを12
5ml加え、37℃で6時間振とうしたのち、0.6mgのプロテ
イナーゼKと1.5mlのクロロホルムを加えて37℃で一晩振
とうした。ここに蒸留水1mlとクロロホルム10mlを添加
し、5分間おだやかに混合したのち、フェノール10mlを
加えてさらに5分間混合し、遠心分離して上層を採取し
た。ここにクロロホルム10mlを加え、5分間おだやかに
混合したのち再び遠心分離して上層を得た。これをもう
一度繰り返し、得られた上層に0.2mlの5M食塩水と12ml
のエタノールを加えて軽く混ぜ、析出したDNAをガラス
棒で巻き取った。これをエタノール中で洗って、3mlのT
EN緩衝液(1mM EDTA, 10mM NaCl, 10mM Tris-HCl, pH7.
6)に溶解した。得られたDNA量は約4.7mgであった。この
DNA 157μｇを制限酵素Sau3AIを用いて部分分解した
後、0.3%アガロースゲル上で電気泳動し、約20kbp以上
のDNA断片を電気溶出により単離した。これをフェノー
ル／クロロホルム抽出したのちエタノール沈殿し、500m
lのTE緩衝液(1mM EDTA, 10mM Tris-HCl, pH7.6)に溶解
した。このうち100mlを20%PEG/2.5M NaCl 100mlと混合
し、遠心分離を行って沈殿を得た。この沈殿物を100ml
のTE緩衝液に溶解し、フェノール、フェノール／クロロ
ホルム、クロロホルムで抽出したのちエタノール沈殿し
て、10mlのTE緩衝液に溶解した。Embodiment 1-1. Sea Wanella sp.SCRC-2140
Preparation of genomic DNA of 6 1.0% peptone, 0.5% yeast extract was dissolved in artificial seawater at 1/2 concentration, 100 ml of a medium adjusted to pH 7.0 was sterilized by heating at 121 ° C for 20 minutes, and then Seawanella sp.SCRC- 21406 (FERM BP-597
9) was inoculated and cultured aerobically at 15 ° C for 24 hours. After culture
Collect the cells with a centrifuge and add 20 ml of SET buffer (50 mM EDT).
A, 20% sucrose, 50 mM Tris-HCl, pH 7.6) once. The obtained cells were frozen in dry ice / acetone for 2 minutes, dissolved in water, and suspended in 2 ml of the same buffer. Add 1mg of lysozyme and 1mg of RNase A here,
After mixing, the mixture was left on ice for 15 minutes. 12 additional 10% SDS
After adding 5 ml and shaking at 37 ° C for 6 hours, 0.6 mg of proteinase K and 1.5 ml of chloroform were added and shaken at 37 ° C overnight. 1 ml of distilled water and 10 ml of chloroform were added thereto and mixed gently for 5 minutes, and then 10 ml of phenol was added and mixed for another 5 minutes, followed by centrifugation to collect the upper layer. 10 ml of chloroform was added thereto, mixed gently for 5 minutes, and then centrifuged again to obtain an upper layer. This was repeated once more, and the obtained upper layer was mixed with 0.2 ml of 5M saline and 12 ml.
Was added and mixed gently, and the precipitated DNA was wound up with a glass rod. Wash this in ethanol and add 3 ml of T
EN buffer (1 mM EDTA, 10 mM NaCl, 10 mM Tris-HCl, pH 7.
Dissolved in 6). The amount of obtained DNA was about 4.7 mg. this
After 157 μg of DNA was partially digested with a restriction enzyme Sau3AI, it was electrophoresed on a 0.3% agarose gel, and a DNA fragment of about 20 kbp or more was isolated by electroelution. This was extracted with phenol / chloroform and precipitated with ethanol.
l of TE buffer (1 mM EDTA, 10 mM Tris-HCl, pH 7.6). 100 ml of the mixture was mixed with 100 ml of 20% PEG / 2.5M NaCl and centrifuged to obtain a precipitate. 100 ml of this precipitate
Was extracted with phenol, phenol / chloroform, and chloroform, followed by ethanol precipitation and dissolution in 10 ml of TE buffer.

【００１６】 実施例１−２． ゲノムDNAライブラリーの作製 ベクターとしてコスミドpWE15（STRATAGENE社製）を用
いた。10μｇのpWE15を制限酵素BamHIを用いて完全分解
し、子牛小腸アルカリフォスファターゼで末端の脱リン
酸化を行ったのち、フェノール、クロロホルムで抽出し
た。これをエタノール沈殿し、10μlのTE緩衝液に溶解
した。上記の方法により得られたベクターDNA1.5μg
と、実施例１で調製したゲノムDNAの制限酵素Sau3AI部
分分解物1.3μgを混合し、T4DNAリガーゼ175Uを添加し
て、4℃で一晩反応させてDNA鎖を連結した。この反応物
の2/5量を常法によりパッケージングしてファージと
し、大腸菌K12/XL1-Blue MR株に感染させた。このファ
ージ感染させた大腸菌液を、50μg/mlのアンピシリン及
びカナマイシンを含むLB寒天平板培地（トリプトン1%、
酵母エキス0.5%、食塩1%、寒天2%）に塗布し、37℃で一
晩培養した。出現したコロニー1687個を同組成培地の保
存用96穴プレートに釣菌し、ライブラリーとした。Embodiment 1-2. Preparation of Genomic DNA Library Cosmid pWE15 (manufactured by STRATAGENE) was used as a vector. 10 μg of pWE15 was completely digested with the restriction enzyme BamHI, dephosphorylated at the ends with calf intestinal alkaline phosphatase, and then extracted with phenol and chloroform. This was precipitated with ethanol and dissolved in 10 μl of TE buffer. 1.5 μg of vector DNA obtained by the above method
And 1.3 μg of the genomic DNA restriction enzyme Sau3AI partially degraded product prepared in Example 1 were mixed, 175 U of T4 DNA ligase was added, and the mixture was reacted at 4 ° C. overnight to link the DNA chains. Two-fifths of this reaction product was packaged by a conventional method to form a phage, and the phage was infected with E. coli K12 / XL1-Blue MR strain. The phage-infected E. coli solution was added to an LB agar plate medium containing 50 μg / ml ampicillin and kanamycin (1% tryptone,
(Yeast extract 0.5%, salt 1%, agar 2%) and cultured at 37 ° C overnight. 1687 colonies that appeared were picked on a 96-well plate for storage of the same composition medium, and used as a library.

【００１７】 実施例１−３． コロニーハイブリダイゼーション １−３−１． メンブレンの作製 実施例１−２で作成したシーワネラsp.SCRC-21406のゲ
ノムライブラリーのコロニー約1687個を96個ずつ100mM
アンピシリン及び50mMカナマイシンを含むLB寒天培地に
拾い、37℃で1晩培養した。生育後、ナイロンメンブレ
ン（Hybond^TMN、アマシャム製）にコロニーを写し取っ
た。このメンブレンを変性溶液（0.5NNaOH、1.5MNaCl）
で7分間、中和溶液（1.5MNaCl、0.5MTris-HCl,pH7.2、1
mMNa₂EDTA）で3分間2回処理した後、2xSSC（30mMクエン
酸ナトリウム、300mM塩化ナトリウム）で洗い風乾し
た。Embodiment 1-3. Colony hybridization 1-3-1. Preparation of Membrane Approximately 1687 colonies of the genomic library of Shiwanella sp.SCRC-21406 prepared in Example 1-2 were each 96 mM at 100 mM.
The cells were picked up on an LB agar medium containing ampicillin and 50 mM kanamycin, and cultured at 37 ° C. overnight. After growth, colonies were copied onto a nylon membrane (Hybond ^™ N, manufactured by Amersham). Use this membrane as a denaturing solution (0.5NNaOH, 1.5M NaCl)
For 7 minutes in a neutralization solution (1.5 M NaCl, 0.5 M Tris-HCl, pH 7.2, 1
Then, the cells were treated twice with 3 mM for 3 minutes, washed with ₂ × SSC (30 mM sodium citrate, 300 mM sodium chloride) and air-dried.

【００１８】１−３−２． DNAプローブの作製 DNAプローブ作製の鋳型DNAとして、ＥＰＡ産生海洋細菌
シーワネラsp.SCRC-2874株の有するＥＰＡ生合成系遺伝
子群（特開平8-242867に記載）のORF9のSpeI(31447)-Nh
eI (32522)断片を使用した。ランダムプライマーDNAラ
ベリングキットVer.2（タカラ製）を用いて、上記DNA断
片25ngを仕様書に従って[a-³²P]ラベルした。DNAに取り
込まれなかった遊離の[a-³²P]dCTPをMicroSpin^TMS-300H
R（ファルマシア製）カラムを用いて除き、精製してDNA
プローブとした。1-3-2. Preparation of DNA probe As a template DNA for preparing a DNA probe, SpeI (31447) -Nh of ORF9 of an EPA biosynthesis gene group (described in JP-A-8-242867) of an EPA-producing marine bacterium Shiwanella sp.
The eI (32522) fragment was used. Using a random primer DNA labeling kit Ver.2 (manufactured by Takara), 25 ng of the above DNA fragment was labeled with [a- ³² P] according to the specifications. Free [a- ³² P] dCTP not incorporated into DNA was added to MicroSpin ^™ S-300H
R (Pharmacia) column, remove and purify DNA
A probe was used.

【００１９】１−３−３． ハイブリダイゼーション 上記１−３−１で作製したメンブレンを50%ホルムアミ
ド、5xSSCP（0.1M リン酸ナトリウム緩衝液、pH6.8、75
mMクエン酸ナトリウム、0.5M塩化ナトリウム）、5mg/ml
スキムミルク、ソニケーションした鮭の***のDNA250μ
g/ml及び0.5%SDSを含むプレハイブリダイゼーション溶
液に浸して42℃で3時間以上置きプレハイブリダイゼー
ションを行った。その後、プレハイブリダイゼーション
溶液を捨て、１−３−２で作製したプローブを加えたハ
イブリダイゼーション溶液（プレハイブリダイゼーショ
ン溶液に10%dextransulfateを加えたもの）にメンブレ
ンを浸して1晩42℃で置きハイブリダイゼーションを行
った。上記のように処理を行ったメンブレンを0.1％SDS
を含む2 x SSC溶液を用いて5分間、新しい同溶液に交換
して10分間振盪しながら室温で洗った。その後、0.1％S
DSを含む0.1x SSC溶液で65℃、10分間洗浄した。洗い終
わったメンブレンはラップフィルムに包み、オートラジ
オグラフィーまたはBAS-1500（富士写真フィルム製）に
よる画像解析を行ってアイソトープの分布を調べた。そ
の結果、40個の陽性クローンが得られた。1-3-3. Hybridization The membrane prepared in the above 1-3-1 was mixed with 50% formamide, 5xSSCP (0.1 M sodium phosphate buffer, pH 6.8, 75
mM sodium citrate, 0.5M sodium chloride), 5mg / ml
Skim milk, sonicated salmon sperm DNA 250μ
Prehybridization was performed by immersing in a prehybridization solution containing g / ml and 0.5% SDS at 42 ° C. for 3 hours or more. Thereafter, the prehybridization solution is discarded, and the membrane is immersed in a hybridization solution (10% dextransulfate added to the prehybridization solution) to which the probe prepared in 1-3-2 has been added. Hybridization was performed. 0.1% SDS of membrane treated as above
Was washed with a 2 × SSC solution containing for 5 minutes, and replaced with the same solution, and washed at room temperature with shaking for 10 minutes. Then 0.1% S
The plate was washed with a 0.1 × SSC solution containing DS at 65 ° C. for 10 minutes. The washed membrane was wrapped in a wrap film, and the distribution of isotopes was examined by autoradiography or image analysis using BAS-1500 (manufactured by Fuji Photo Film). As a result, 40 positive clones were obtained.

【００２０】 １−３−４． 陽性クローンのコスミドの分析 上記１−３−３で得られた陽性クローンよりコスミドを
調製し、制限酵素EcoRIを用いて切断後アガロース電気
泳動を行った。泳動後アガロースゲルのエチジウムブロ
マイド染色を行ってDNAバンドを検出し、挿入断片の入
っている29個のクローンを選んだ。1-3-4. Analysis of Cosmids of Positive Clones Cosmids were prepared from the positive clones obtained in the above 1-3-3, digested with EcoRI, and then subjected to agarose electrophoresis. After electrophoresis, ethidium bromide staining of an agarose gel was performed to detect a DNA band, and 29 clones containing the inserted fragment were selected.

【００２１】 実施例１−４．ドットハイブリダイゼーション １−４−１． メンブレンの作製 実施例１−３で得られた29個の陽性クローンのコスミド
DNA溶液各1μlを含む2x SSC溶液55.5μlを調製し、95℃
で5分間加熱処理した後2分間氷冷したものを1μlずつナ
イロンメンブレンにスポットした。このメンブレンを変
性溶液で1分間、中和溶液で1分間処理した後、風乾させ
てラップフィルムに包み、UVランプで4分間照射してDNA
をメンブレンに固定した。Embodiment 1-4. Dot hybridization 1-4-1. Preparation of membrane Cosmid of 29 positive clones obtained in Example 1-3
Prepare 55.5 μl of 2x SSC solution containing 1 μl of each DNA solution, and store at 95 ° C.
The mixture was heat-treated for 5 minutes and then ice-cooled for 2 minutes, and 1 μl of the mixture was spotted on a nylon membrane. After treating this membrane with a denaturing solution for 1 minute and a neutralizing solution for 1 minute, air-dry it, wrap it in a wrap film, and irradiate it with a UV lamp for 4 minutes to remove DNA.
Was fixed to the membrane.

【００２２】１−４−２． DNAプローブの作製 DNAプローブ作製の鋳型DNAとして、実施例１−３−２の
ORF9のSpeI-NheI断片の他に、ＥＰＡ生合成遺伝子のORF
6中のDraIII(13887)-DraIII(16031)断片、EcoRI(17922)
-EcoRI (18252)断片、ORF7のEcoRI-XbaI断片（約1k
b）、ORF8のPstI-PstI断片（約1.2kb）を用いた。DNA断
片のラべリング及び精製は実施例１−３−２と同様に行
った。1-4-2. Preparation of DNA probe As a template DNA for preparing a DNA probe, the DNA of Example 1-3-2 was used.
In addition to the SpeI-NheI fragment of ORF9, the ORF of EPA biosynthesis gene
DraIII (13887) -DraIII (16031) fragment in 6, EcoRI (17922)
-EcoRI (18252) fragment, ORF7 EcoRI-XbaI fragment (about 1 k
b), a PstI-PstI fragment (about 1.2 kb) of ORF8 was used. Labeling and purification of the DNA fragment were performed in the same manner as in Example 1-3-2.

【００２３】１−４−３． ハイブリダイゼーション ハイブリダイゼーションは、実施例１−３−３と同様の
方法で行った。メンブレンの洗浄は、0.1％SDSを含む2
x SSC溶液については、実施例１−３−３と同様の方法
で行った。その後の洗浄については以下のように行っ
た。DNAプローブがORF6のDraIII-DraIII断片、ORF8のPs
tI-PstI断片及びORF9のSpeI-NheI断片の場合は、0.1％S
DSを含む0.1x SSC溶液で65℃10分間2回、ORF7のEcoRI-X
baI断片の場合は、0.1％SDSを含む0.2x SSC溶液で55℃1
0分間の洗浄を行った。洗い終わったメンブレンは、実
施例１−３−３と同様に処理を行い解析した結果、21種
のクローンより得たコスミドがORF9のSpeI-NheI断片と
ハイブリダイズし、そのうち4種が非常に強くハイブリ
ダイズした。この4種のクローンのうち、クローン10C7
のみが他の3種類のDNAプローブすべてとハイブリダイズ
した。1-4-3. Hybridization Hybridization was performed in the same manner as in Example 1-3-3. Membrane washes containing 0.1% SDS2
The xSSC solution was performed in the same manner as in Example 1-3-3. The subsequent washing was performed as follows. DNA probe is DraIII-DraIII fragment of ORF6, Ps of ORF8
In the case of the tI-PstI fragment and the SpeI-NheI fragment of ORF9, 0.1% S
0.1x SSC solution containing DS twice at 65 ° C for 10 minutes, ORF7 EcoRI-X
In the case of the baI fragment, use a 0.2x SSC solution containing 0.1% SDS at 55 ° C1
A 0 minute wash was performed. The washed membranes were treated and analyzed in the same manner as in Example 1-3-3. As a result, cosmids obtained from 21 clones hybridized with the SpeI-NheI fragment of ORF9, and 4 of them were very strong. Hybridized. Of these four clones, clone 10C7
Only hybridized with all three other DNA probes.

【００２４】実施例１−５． サザンブロットハイブリ
ダイゼーション １−５−１． メンブレンの作製 実施例１−４−３ですべてのDNAプローブとハイブリダ
イズすることがわかった陽性クローン、10C7のコスミド
DNAをp10C7と命名した。このp10C7を制限酵素EcoRIで切
断し、0.7％アガロースゲル （ SeaKem GTG、FMC製）を
用いて100Vで約4時間電気泳動を行った。泳動後、エチ
ジウムブロマイド染色を行ってDNAバンドを検出したと
ころ、６本のバンドが観察された。これらのバンドを分
子量の大きいものから順にE1〜E6と命名した。観察後の
ゲルを蒸留水で洗浄し、0.25NHCl中で15分間振盪したの
ち蒸留水で洗浄して変性溶液中で30分間振盪後、ブロッ
ティング溶液（0.25NNaOH、1.5MNaCl）に浸し、ナイロ
ンメンブレンに1時間減圧ブロットを行った。メンブレ
ンを2 x SSCで洗った後に風乾した。Embodiment 1-5. Southern blot hybridization 1-5-1. Preparation of membrane Cosmid of 10C7, a positive clone found to hybridize with all DNA probes in Example 1-4-3
The DNA was named p10C7. This p10C7 was digested with restriction enzyme EcoRI, and electrophoresed at 100 V for about 4 hours using 0.7% agarose gel (SeaKem GTG, manufactured by FMC). After electrophoresis, ethidium bromide staining was performed to detect DNA bands. As a result, six bands were observed. These bands were named E1 to E6 in ascending order of molecular weight. After observation, the gel was washed with distilled water, shaken in 0.25 NHCl for 15 minutes, washed with distilled water, shaken in a denaturing solution for 30 minutes, immersed in a blotting solution (0.25 NNaOH, 1.5 M NaCl), and placed on a nylon membrane. A vacuum blot was performed for 1 hour. The membrane was washed with 2 x SSC and air dried.

【００２５】１−５−２． ハイブリダイゼーション DNAプローブは実施例１−４−２で作成したものを用い
た。ハイブリダイゼーション及びメンブレンの洗浄は、
実施例１−４−３と同様の方法で行った。洗い終わった
メンブレンは、実施例１−４−３と同様に処理を行い解
析した。その結果、コスミドp10C7の制限酵素EcoRI切断
断片は、ＥＰＡ 生合成遺伝子のORF6,8,9のDNAプローブ
とのハイブリダイズが認められた。すなわち、コスミド
p10C7はシーワネラsp.SCRC-2874株のＥＰＡ 生合成遺伝
子群の塩基配列と相同性を有することが示された。1-5-2. Hybridization A DNA probe used in Example 1-4-2 was used. Hybridization and membrane washing
It carried out by the method similar to Example 1-4-3. The washed membrane was processed and analyzed in the same manner as in Example 1-4-3. As a result, the restriction enzyme EcoRI digested fragment of cosmid p10C7 hybridized with the DNA probes of ORF6, 8, and 9 of the EPA biosynthesis gene. That is, cosmid
p10C7 was shown to have homology to the base sequence of the EPA biosynthesis gene group of Shiwanella sp. SCRC-2874 strain.

【００２６】実施例１−６． コスミドp10C7の制限
酵素地図の作成 形質転換菌XL1-Blue MR/p10C7株より調製したコスミドp
10C7のゲノムDNA挿入部分を種々の制限酵素で切断し、
制限酵素切断地図を作成した（図１）。Embodiment 1-6. Creation of restriction map of cosmid p10C7 Cosmid p prepared from transformant XL1-Blue MR / p10C7
Cut the genomic DNA insert of 10C7 with various restriction enzymes,
A restriction enzyme cleavage map was created (FIG. 1).

【００２７】 実施例１−７． 形質転換菌JM109/p10C7の作成 コスミドp10C7を用いて、大腸菌JM109株を形質転換して
JM109/p10C7を得た。本菌株は、工業技術院生命工学工
業技術研究所に、平成１０年１１月１８日にＦＥＲＭ
Ｐ−１７０５８として寄託されている。Embodiment 1-7. Preparation of transformant JM109 / p10C7 Using cosmid p10C7, E. coli JM109 was transformed.
JM109 / p10C7 was obtained. This strain was submitted to the National Institute of Bioscience and Human Technology by the National Institute of Advanced Industrial Science and Technology on November 18, 1998.
Deposited as P-17058.

【００２８】実施例１−８． 配列の解析 コスミドp10C7のゲノムDNA挿入部分のうち、図１の白色
部分の全塩基配列を配列番号：１に示す。この塩基配列
中には４個のオープンリーデイングフレーム(ORF)：a〜
dを特定することができ、これらのそれぞれの塩基配列
及び対応するアミノ酸配列を配列番号：２、４、６、８
に示す。全塩基配列（配列番号：１）とORFa〜d（配列
番号：２、４、６、８）との関係は表１の通りである。Embodiment 1-8. Sequence analysis The entire base sequence of the white part in FIG. 1 in the genomic DNA insertion part of cosmid p10C7 is shown in SEQ ID NO: 1. This base sequence contains four open reading frames (ORFs): a to
d can be specified, and their respective nucleotide sequences and corresponding amino acid sequences are represented by SEQ ID NOs: 2, 4, 6, 8
Shown in Table 1 shows the relationship between the entire base sequence (SEQ ID NO: 1) and ORFs a to d (SEQ ID NOs: 2, 4, 6, and 8).

【００２９】 表１ ──────────────────────────────── 配列番号 配列の長さ(bp) 配列番号１に対する配列の位置 ──────────────────────────────── ２ 7875 483-8357 ４ 2631 8530-11160 ６ 6111 11212-17322 ８ 1614 17579-19192 ────────────────────────────────TABLE 1 SEQ ID NO: Sequence length (bp) for SEQ ID NO: 1 Sequence position ────────────────────────────────2 7875 483-8357 4 2631 8530-11160 6 6111 11212-17322 8 1614 17579-19192 ────────────────────────────────

【００３０】前記種々のORFの塩基配列より推定される
アミノ酸配列（配列番号３、５、７、９）と、ＥＰＡ生
合成酵素群をコードする遺伝子群の各ORFの塩基配列よ
り推定されるアミノ酸配列（特開平8-242867に記載）と
を比較したところ、ある程度の相同性を有することが見
出された。その結果を表２に示す。The amino acid sequences deduced from the base sequences of the various ORFs (SEQ ID NOS: 3, 5, 7, 9) and the amino acid sequences deduced from the base sequences of the ORFs of the genes encoding the EPA biosynthetic enzymes Comparison with the sequence (described in JP-A-8-242867) revealed that the sequence had some homology. Table 2 shows the results.

【００３１】 表２ ─────────────────────────────────── 配列番号 10C7のORF EPA生合成酵素群のORF 相同性（％） （アミノ酸数） （アミノ酸数） ─────────────────────────────────── ３ ａ(2625) ６(2756) 47.3 ５ ｂ(877) ７(780) 25.9 ７ ｃ(2037) ８(2004) 49.6 ９ ｄ(538) ９(543) 62.6 ───────────────────────────────────TABLE 2 ORF EPA biosynthetic enzymes of SEQ ID NO: 10C7 ORF homology (%) (number of amino acids) (number of amino acids) ア ミ ノ 酸3a (2625) 6 (2756) 47.35 b (877) 7 (780) 25.9 7 c (2037) 8 (2004) 49.6 9 d (538) 9 (543) 62.6 ────────── ─────────────────────────

【００３２】この結果より、配列番号２、４、６、８
（コスミド10C7に含まれるORFa,b,c,d）のコードするタ
ンパクは、ＥＰＡ生合成酵素群をコードするORF6,7,8,9
の産物とそれぞれ機能的に類似していることが予想され
た。From the results, SEQ ID NOs: 2, 4, 6, 8
(ORFa, b, c, d contained in cosmid 10C7) is encoded by ORFs 6,7,8,9 encoding EPA biosynthetic enzymes.
Were expected to be functionally similar to the respective products.

【００３３】実施例２−１． コスミド10C7のゲノムDN
A挿入部分の制限酵素断片のサブクローニング コスミド10C7のゲノムDNA挿入部分のORFbを含むEcoRI(2
391)-EcoRI(14761)断片をプラスミドpSTV28（宝酒造社
製）のEcoRI部位に挿入して、プラスミドEco-Eco/pSTV2
8を作成した。また、ORFdを含むPstI(16802)-PstI(2226
3)断片をプラスミドpSTV28のPstI部位に挿入して、プラ
スミドPst-Pst/pSTV28を作成した。また、ORFcを含むEc
oRV(11172)-PvuI(17457)断片を末端平滑化後プラスミド
pSTV29のSmaI部位に挿入して、プラスミドEco-Pvu/pSTV
29を作成した。各サブクローンの挿入断片の位置を図２
に示す。Embodiment 2-1. Genomic DN of cosmid 10C7
Subcloning of restriction enzyme fragment of A insertion part EcoRI (2) containing ORFb of genomic DNA insertion part of cosmid 10C7
The 391) -EcoRI (14761) fragment was inserted into the EcoRI site of plasmid pSTV28 (manufactured by Takara Shuzo) and the plasmid Eco-Eco / pSTV2
Created 8. Also, PstI (16802) -PstI (2226) containing ORFd
3) The fragment was inserted into the PstI site of plasmid pSTV28 to create plasmid Pst-Pst / pSTV28. Ec containing ORFc
oRV (11172) -PvuI (17457) fragment blunt-ended plasmid
Inserted into the SmaI site of pSTV29, the plasmid Eco-Pvu / pSTV
Created 29. Figure 2 shows the location of the insert in each subclone.
Shown in

【００３４】実施例２−２． ＥＰＡ生合成系遺伝子群
のORF9,10欠失クローンの作成 ＥＰＡ生合成酵素群をコードする遺伝子群を含むプラス
ミドpEPA（特開平8-242867に記載）のXhoI(5660)-SpeI
(31446)断片（ORF2〜8を含む）をプラスミドpBluescrip
t（STRATAGENE社製）のXhoI-SpeI部位に連結して、pXS-
BS2を作成した。pXS-BS2の挿入断片の位置を図３に示
す。本プラスミドはＥＰＡの生合成に必須な遺伝子のう
ちのORF9を欠失している。Embodiment 2-2. Preparation of ORF9,10 Deletion Clones of EPA Biosynthesis System Genes XhoI (5660) -SpeI of plasmid pEPA (described in JP-A-8-242867) containing genes encoding EPA biosynthesis enzymes
(31446) fragment (including ORFs 2-8) was transferred to plasmid pBluescrip
t (manufactured by STRATAGENE) and ligated to the XhoI-SpeI site,
BS2 was created. The position of the inserted fragment of pXS-BS2 is shown in FIG. This plasmid lacks ORF9 among the genes essential for EPA biosynthesis.

【００３５】実施例２−３． ＥＰＡ生合成系遺伝子群
のORF2,4,5,8,10欠失クローンの作成 ＥＰＡ産生能を有するプラスミドΔ2,4,5,10/pNEB（WO9
8/01565に記載）からPstI(24813)-EcoT22I(28946)（ORF
8の一部）部分を欠失させて、Δ2,4,5,8,10/pNEBを作成
した。Δ2,4,5,8,10/pNEBの挿入断片の位置を図３に示
す。本プラスミドはＥＰＡの生合成に必須な遺伝子のう
ちのORF8を欠失している。Embodiment 2-3. Preparation of ORF2,4,5,8,10 deletion clone of EPA biosynthesis gene group Plasmid Δ2,4,5,10 / pNEB (WO9
8/01565) to PstI (24813) -EcoT22I (28946) (ORF
8) was deleted to create Δ2,4,5,8,10 / pNEB. The position of the inserted fragment of Δ2,4,5,8,10 / pNEB is shown in FIG. This plasmid lacks ORF8 among the genes essential for EPA biosynthesis.

【００３６】実施例２−４． ORF7またはORF9欠失ＥＰ
Ａ生合成系遺伝子群の相補によるＥＰＡ生産能の回復 実施例２−１で作成したプラスミドのうちの１種を導入
した大腸菌JM109株を、さらにpEPAΔ7（pEPAからORF7の
一部であるSalI(22260)-NruI(23845)部分を欠失させた
プラスミド、特開平8-242867に記載）または実施例２−
２で作成したpXS-BS2を用いて形質転換した。これらの
形質転換株と、pEPAΔ7またはpXS-BS2のみを有する形質
転換株を、50μg/mlのアンピシリン及びクロラムフェニ
コールを含むLB培地5mlに接種し、25℃で培養した。遠
心分離により菌体を得、１回洗浄したのち凍結乾燥し、
塩化水素飽和のメタノール2mlに溶解して密閉容器に入
れ、80℃で１時間保温して脂肪酸をメチルエステル化し
た。放冷後、ヘキサン2mlで３回抽出し、ヘキサン層を
乾固して10mlのメタノールに溶解した。この溶解物の一
部をガスクロマトグラフィーにより分析したところ、Ｅ
ＰＡのピークが観察された。それぞれの形質転換株の保
有するプラスミドの組み合わせと、ピークの面積比から
算出した菌体の全脂肪酸エステル中におけるＥＰＡメチ
ルエステルの割合を表３に示す。Embodiment 2-4. ORF7 or ORF9 deleted EP
Restoration of EPA production ability by complementation of A biosynthesis gene group Escherichia coli JM109 strain into which one of the plasmids prepared in Example 2-1 was introduced was further transformed with pEPAΔ7 (SalI (22260, a part of ORF7 from pEPA) ) -NruI (23845) plasmid deleted, described in JP-A-8-242867) or Example 2-
Transformation was performed using pXS-BS2 prepared in Step 2. These transformants and transformants having only pEPAΔ7 or pXS-BS2 were inoculated into 5 ml of LB medium containing 50 μg / ml ampicillin and chloramphenicol, and cultured at 25 ° C. Cells were obtained by centrifugation, washed once, freeze-dried,
The fatty acid was dissolved in 2 ml of methanol saturated with hydrogen chloride, placed in a closed container, and kept at 80 ° C. for 1 hour to methylesterify the fatty acid. After cooling, the mixture was extracted three times with 2 ml of hexane, and the hexane layer was dried and dissolved in 10 ml of methanol. When a part of this lysate was analyzed by gas chromatography, E
A PA peak was observed. Table 3 shows the combinations of plasmids possessed by the respective transformants and the ratio of EPA methyl ester in all fatty acid esters of the bacterial cells calculated from the peak area ratio.

【００３７】 表３ ─────────────────────────────────── 形質転換株 プラスミド１ プラスミド２ EPA(%) ─────────────────────────────────── １ - pEPAΔ7 0 ２ - pXS-BS2 0 ３ Eco-Eco/pSTV28 pEPAΔ7 0.3 ４ Pst-Pst/pSTV28 pXS-BS2 12.1 ────────────────────────────────────Table 3 ─────────────────────────────────── Transformed strain Plasmid 1 Plasmid 2 EPA (% ) ─────────────────────────────────── 1-pEPAΔ7 0 2-pXS-BS2 0 3 Eco-Eco / pSTV28 pEPAΔ7 0.3 4 Pst-Pst / pSTV28 pXS-BS2 12.1 ────────────────────────────────────

【００３８】上記の結果より、ＥＰＡ生合成系遺伝子群
のうちのORF7またはORF9を欠失させたクローンのＥＰＡ
産生能が、コスミドp10C7由来のDNA断片の存在によって
回復することが示された。すなわち、コスミドp10C7中
にはORF7及びORF9と同様の機能を有する、高度不飽和脂
肪酸の生合成に関与する遺伝子が含まれていることが示
された。From the above results, it was found that the clones lacking ORF7 or ORF9 in the EPA biosynthesis gene group had the EPA
It was shown that the productivity was restored by the presence of the DNA fragment derived from cosmid p10C7. In other words, it was shown that cosmid p10C7 contains genes involved in the biosynthesis of polyunsaturated fatty acids having the same functions as ORF7 and ORF9.

【００３９】実施例２−５． ORF8欠失ＥＰＡ生合成系
遺伝子群の相補によるＥＰＡ生産能の回復及びＤＨＡ生
産 実施例２−１で作成したプラスミドEco-Pvu/pSTV29を導
入した大腸菌JM109株を、さらに実施例２−３で作成し
たプラスミドΔ2,4,5,8,10/pNEBを用いて形質転換し、
アンピシリン及びクロラムフェニコールを含む寒天平板
培地を用いて選別を行って、２種のプラスミドを有する
組み換え体JM109/p3,6,7,9/pcを得た。この形質転換株
を、実施例２−４と同様にして18℃で培養し、菌体の脂
肪酸組成をガスクロマトグラフィーにより分析した。そ
の結果、ＥＰＡのピークが観察され、その全脂肪酸エス
テル中における割合は、ピークの面積比から約22.0%と
算出された。一方、ガスクロマトグラフィー上でEPAメ
チルエステルよりも高分子側に新たなピークが観察され
た。このピークの物質の全脂肪酸エステル中における割
合は、ピークの面積比から約1.5%と算出された。GC-MS
分析の結果、このピークの物質の分子量は342であり、
ＤＨＡ(22:6)メチルエステルの値と一致し、各フラグメ
ントのピークは標品のそれと一致したことから、本物質
はＤＨＡメチルエステルと同定された。なお、MSフラグ
メントピークは以下の通りであった。Mass : 342(M+),
327, 311, 299, 286, 273, 241, 215, 206, 173, 159,
145, 131, 119, 105, 91, 79, 67, 55, 41, 27.Embodiment 2-5. Restoration of EPA-producing ability and DHA production by complementation of ORF8-deleted EPA biosynthesis gene group. Escherichia coli JM109 strain into which plasmid Eco-Pvu / pSTV29 prepared in Example 2-1 was introduced was further prepared in Example 2-3. Transformation using the plasmid Δ2,4,5,8,10 / pNEB,
Selection was carried out using an agar plate medium containing ampicillin and chloramphenicol to obtain recombinant JM109 / p3, 6, 7, 9 / pc having two types of plasmids. This transformed strain was cultured at 18 ° C. in the same manner as in Example 2-4, and the fatty acid composition of the cells was analyzed by gas chromatography. As a result, an EPA peak was observed, and its ratio in the total fatty acid esters was calculated to be about 22.0% from the peak area ratio. On the other hand, on gas chromatography, a new peak was observed on the higher polymer side than EPA methyl ester. The ratio of this peak substance to the total fatty acid esters was calculated to be about 1.5% from the peak area ratio. GC-MS
As a result of analysis, the molecular weight of the substance at this peak was 342,
The substance was identified as DHA methyl ester because the value matched that of DHA (22: 6) methyl ester and the peak of each fragment matched that of the standard. The MS fragment peak was as follows. Mass: 342 (M +),
327, 311, 299, 286, 273, 241, 215, 206, 173, 159,
145, 131, 119, 105, 91, 79, 67, 55, 41, 27.

【００４０】上記の結果より、ORF8を欠失させたＥＰＡ
生合成系遺伝子群を有するクローンのＥＰＡ産生能が、
コスミドp10C7由来のDNA断片の存在によって回復し、さ
らにＤＨＡの生産能も獲得したことが示された。すなわ
ち、コスミドp10C7中にはORF8と同様の機能を有し、か
つＤＨＡの生合成に関与する遺伝子が含まれていること
が示された。From the above results, EPA lacking ORF8
The EPA-producing ability of the clone having the biosynthetic gene group is
It was shown that the DNA fragment was recovered by the presence of the DNA fragment derived from cosmid p10C7, and that it also acquired the ability to produce DHA. That is, it was shown that cosmid p10C7 contains a gene having the same function as ORF8 and involved in DHA biosynthesis.

【００４１】[0041]

【配列表】[Sequence list]

【００４２】 <110> Sagami Chemical Reserach Center, Japan Bioindustry Association, Director-General of Agency of Industrial Science and Technology. <120> Genes coding for biosynthetic enzyme group for icosapentaenoic aci d and / or docosahexaenoic acid. <130> KJ0071 <160> 9<110> Sagami Chemical Reserach Center, Japan Bioindustry Association, Director-General of Agency of Industrial Science and Technology. <120> Genes coding for biosynthetic enzyme group for icosapentaenoic acid and and / or docosahexaenoic acid. <130> KJ0071 < 160> 9

【００４３】 <210> 1 <211> 23821 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 gtgagatatc agactaattt tagcttatta atttgttagt tgtttatatt taataaagac 60 gtaatttaac ttaaatgcaa ttaattatgg cgtaaataga gtgaaaacat ggctaatatt 120 cactaagtcc taaattttat ataaagttta atctgttatt ttagcgttta cctggtctaa 180 tcagtgaggt ttttagccat tattggtggg attgaagtga ttttcaaagc tatgtatatt 240 attgcaaata taaattgtaa caattaagac tttgaacact tgagttcaat ttcaaattga 300 ttggcataaa atttaaaaca gctaaatcta cctcaatcat tttagcaaat gtatgcaggt 360 agattttttt cgccatttaa gagtacactt gtacgctagg tttttattta gtgtgcaaat 420 gaacgttttg atgagcattg tttttagtgc acaaaataga tccttacagg agcaataacg 480 caatggctaa aaagaacacc acatcgatta agcacgccaa ggatgtgtta agtagtgatg 540 atcaacagtt aaattctcgc ttgcaagaat gtccgattgc catcattggt atggcatcgg 600 tttttgcaga tgctaaaaac ttggatcaat tctgggataa catcgttgac tctgtggacg 660 ctattattga tgtgcctagc gatcgctgga acattgacga ccattactcg gcagataaaa 720 aagcagctga caagacatac tgcaaacgcg gcggtttcat tccagagctt gattttgatc 780 cgatggaatt tggtttaccg ccaaatatcc tcgagttaac tgacatcgct caactgttgt 840 cattaattgt tgctcgtgat gtattaagtg atgctggcat tggtagtgat tatgaccatg 900 ataaaattgg tatcacgctg ggtgtcggtg gtggtcagaa acaaatttcg ccattaacgt 960 cgcgcctaca aggtccggta ttagaaaaag tattaaaagc ttcaggcatt gatgaagatg 1020 atcgcgctat gatcatcgac aaatttaaaa aggcctacat cggctgggaa gagaactcat 1080 tcccgggcat gctaggtaac gtcattgctg gccgtatcgc caatcgtttt gattttggtg 1140 gtactaactg tgtggttgat gcggcatgtg ccggctccct tgcagcagta aaaatggcga 1200 tttcggactt acttgaatat cgctctgaag taatgatctc gggtggcgta tgttgtgata 1260 actcgccatt catgtatatg tcattctcaa aaacaccggc atttaccacc aacgatgata 1320 tccgtccgtt tgatgacgat tcaaaaggca tgctcgttgg tgaaggtatc ggcatgatgg 1380 cgtttaaacg ccttgaagat gctgaacgtg acggcgacaa aatttattcg gtactgaaag 1440 gtatcggtac atcttcagat ggtcgtttca aatctattta tgctccgcgc ccagatggcc 1500 aagcaaaggc gctgaaacgt gcttatgaag atgcaggttt tgcccctgaa acttgtggac 1560 taattgaagg ccatggtaca ggtactaaag ccggtgatgc agcagaattt gctggtctaa 1620 ctaaacactt tggcgctgcc agtgatgaaa aacaacatat cgcattaggc tcagttaaat 1680 cgcaaattgg tcatacaaaa tctgcggctg gctctgcggg tatgattaag gcggcattag 1740 ccctgcatca taaaatccta ccagcaacaa ttcatatcga taagccaagc gaagccttgg 1800 atatcaaaaa cagcccgtta tacctaaaca gcgaaacgcg tccttggatg ccacgtgaag 1860 atggtattcc acgtcgtgct ggtatcagct catttggttt tggtggtacg aacttccata 1920 ttattttaga agagtaccgt ccaggtcacg atagtgcata tcgcttaaat tcagtgagtc 1980 aaactgtgtt gatctcggca aacgatcaac aaggtatcat tgcagaatta aataactggc 2040 gtactaaact ggctgtcgat gccgaacagc aagcatttgt gtttaacgac ttagtgacaa 2100 cgtggccatt aaaaacacca tcagttaatc aagctcgttt aggttttgtt gcgcgtaatg 2160 ccaacgaagc gattgcgatg attgatacgg cattaaaaca atttaacgct aatgcagata 2220 aaaccacatg gtcagtgcct accggggttt actatcgcca agctggtatt gatgcaacag 2280 gtaaagtcgt ggcgctattc tcaggacaag gttcgcaata tgtgaacatg ggtcgagaat 2340 taacctgcaa cttcccaagc atgatggaca gtgctgcggc aatggataaa gaattcagcg 2400 gggctggctt aggtcagtta tctgccgtta ctttcccaat ccctgtttac acggataccg 2460 agcgtaagct acaagaagat caattacgtt taactcaaca tgcacaacca gcgattggta 2520 gtttgagtgt tggtctgttc aagactttcc aacaagcagg ctttaaagct gatttcgctg 2580 ccggtcatag tttcggtgaa ttaacggcat tatgggctgc tgatgtactg agcgaaagcg 2640 attacatgat gttagcgcgt agtcgtggtc aagcaatggc tgcacctgag caacaagatt 2700 ttgatgcggg taagatggcc gctgttgttg gcgatccaaa gcaagtagcc gtcatcattg 2760 atactattga tgatgtctct attgctaact acaactcgaa taaccaagtt gttattgcgg 2820 gtactactgc gcaggttgct gtagcagtta caaccttagg caatgctggt ttcaaagttg 2880 tgccactgcc ggtatccgct gcgttccata cacctttagt gggtcatgct caaaaaccat 2940 ttgctaaagc ggttgatcgc gctaaattta aagcgccaag catccctgta tttgctaatg 3000 gcacaggctt agttcattca agtaaaccga atgacattaa gaaaaacctg aaaaaccaca 3060 tgttagaatc tgttcatttc aatcaagaaa ttgacaacat ctatgctgat ggtggtcgcg 3120 tatttatcga atttggtccg aagaatgtat taactaaatt ggttgaaaac attctcactg 3180 acaaatctga tgtgactgct atcgcggtta atgctaatcc aaaacaacct gctgatgtac 3240 aaatgcgtca agctgcgctg caaatggcag tgcttggtgt cgcattagac aatattgacc 3300 cgtacgacgc agttaagcgc ccacttgttg cgccaaaagc atcgccaatg ttgatgaaat 3360 tatctgcagc gtcttatgtt agtccaaaaa cgaagaaagc gtttgcagat gcattaacgg 3420 atggttggac ggtgaaacaa gccaaagccg caccagttgt tgtgtcacaa ccacaagtga 3480 ttgaaaagat cgttgaagtt gaaaagatag tcgaacgcat tgtcgaagtt gagcgtatcg 3540 ttgaagtaga aaaaattgtc tacgttaatg ctgatggttc acatgcaacg acgaacaata 3600 gcgctattaa taatggcatg catagctctg acgccgatct cgttgcctca attgaacaga 3660 gtgttggtca gtttgttgca caccaacagc aattattaaa tgtacatgaa cagtttatgc 3720 aaggtccaca agactatgca aaaacagtgc aaaacgtact tgctgcgcaa accaataatg 3780 aattaccgga aagtttagac cgtacattgt ctatgtataa cgagttccaa tcagaaacgc 3840 tacgtgtaca tgaaacgtac ctgaacaatc agacgagcag catgaacact atgcttactg 3900 gtgctgaaac tgatgtgcta gtagctcctg tcactcaagt attgaataca cccgttgccg 3960 ctagtcacaa ggcagttgct ccagttattg ctaatacagt gacgaatgtt gtatctagtg 4020 tcagtaataa cgcggcggtt gcagtgcaaa ctgtggcatt agcgcctacg caagaaatcg 4080 ctccagcagt cgttactacg ccagcacccg cattggttgc tattgtggct gaacctgtga 4140 ttgttgcgcc tgttgctaca gaagttgcac caattacatc atcagttaca cctgttgtcg 4200 caactcaagc ggctatcgat gtagcaacta ttaacaaagt aatgttagaa gttgttgctg 4260 ataaaaccgg ttatccaaca gacatgcttg aactgagcat ggacatggaa gctgatttag 4320 gtatcgactc aatcaaacgt gttgaaattc taggcgcagt acaggaattg atccctgact 4380 tacctgagct taaccctgaa gatcttgctg agctacgtac gcttggtgag attgtcgatt 4440 atatgaatgt taaagcgcaa gaggctgctc ctgtatcagc acctgcatct gctggtattg 4500 atttagccca catccaaaac gtaatgttag aagtggttgc agacaaaacc ggttacccaa 4560 ctgacatgct tgaactgagc atggacatgg aagctgactt aggtattgat tcaatcaaac 4620 gtgtggaaat cttaggcgca gtgcaggaga tcatcactga tttacctgag ctaaatcctg 4680 aagacttagc tgagctacgc accttaggtg aaatcgttag ttacatgcaa agcaaagcgc 4740 ccgtcgctga aagtgtgact gttacagatt cagacgttac ttctgtcgca agctctgcac 4800 catctatcga tttgaaccac attcaaacag tgatgatgga tgtagttgca gataagactg 4860 gttatccaac agatatgtta gaacttggca tggacatgga agctgactta ggtatcgatt 4920 caatcaaacg tgtggaaata ttaggcgcag tgcaggagat cattactgat ttacctgagc 4980 taaaccctga agatcttgct gaattacgca cgctaggtga aatcgttagc tacatgcaaa 5040 gcaaagcgcc tgtagctgaa gcgcctgcag ctgtagaaag tgcacctatt ggtgtaacaa 5100 gttcagcacc gtctatcgat ttaaaccaca tccaaactgt gatgatggaa gttgttgcag 5160 acaaaaccgg ttatccagta gacatgttag aacttgctat ggacatggaa gctgaccttg 5220 gtatcgactc aatcaaacgt gtagaaattc taggtgcggt acaggaaatc atcactgact 5280 tacctgagct aaaccctgaa gatcttgctg aactacgtac gttaggtgaa atcgttagtt 5340 acatgcaaag caaagtgccc gtagctgaag cgcctgttgc tgtagaaagt gcacatactg 5400 gtgtaacaag ttcagcaccg tctatcgatt tagaccatat ccaaaacgta atgatggatg 5460 ttgttgctga taagactggt tatcctgcca atatgcttga actagcaatg gacatggaag 5520 ctgaccttgg tattgattca atcaagcgtg ttgaaattct aggcgcggta caggagatca 5580 ttactgattt acctgaacta aacccagaag acttagcgga actacgtacg ttagaagaaa 5640 ttgtaactta catgcagagt aaagctcgtg gtgttactgc aaatgtagta gctaatcatg 5700 aacctgaaaa aagcgcagtg tcagatgcat ttatgcagaa cagcgttgct actatcacgg 5760 cagctgtagc gccaaaagca gagtttcaac cagcgccgag cgcaacggtt gctatctcgc 5820 gtctaagctc tatcagtaaa ataagccaaa attgccaagg cgctaatgcc ttaatcgtgg 5880 ctgatggcac tgataatgca gtattactcg cagaccacct actgcaaact ggctggaatg 5940 taactgcatt gcaaccaact tgggtagcag taacaacgac gaaagcattt aatcaatcag 6000 taaacctggt gagcttaaat ggcgttgatg aaactgaaat caacaacatc attgctgcta 6060 acgcacaatt ggatgcagtt atctatctgc acgccagtag cgaaattaat gctattgaat 6120 acccacaagc atctaagcaa ggcctgatgt tagccttctt attagcgaaa ttgagtaaag 6180 taactcaagc cgctaaagtg cgtggttctt tcatggtagt tactcagcag ggcggttcac 6240 ttggttttag caatgacgca caagctgacc tagtacaggc agacttagta cagacagacc 6300 ttgtacaaag cggcttaaac ggtttagtta aaacactgtc acacgaatgg gataacgtat 6360 tctgtcgtgc ggtagatatg ccaacatcat tagctgcagc gcaagtagca actcttgttt 6420 gtgatgaact gtttgacgca gatagcgtgt taactgaagt aggttatcaa caagcagagc 6480 aaaatggtgt tgcccgcatt accttaactg gtgttgcgac tgacagttat gcactgactg 6540 ctggtaataa cattgatgct aattcggtat tcttagtgag tggtggcgca aaaggcgtaa 6600 cagcccattg cgtagtacgt atcgctgaag agtatcaagc taagttcatc ttattaggac 6660 gctcaaaatt ttcaaatgat gagccgagct gggcaaacgg tattactgaa gaagcggcat 6720 taaagaaagc agcaatgcag gctttaattg cagcaggtga taaaccaacg cccgttaaga 6780 tcgtacagtt aatcaaacca attcaagcta atcgtgaaat tgcgcaaact ttgtctgcaa 6840 ttaccgctgc tggtggccaa gctgaatatg tttctgcaga tgtaaccaat gcagcaagcg 6900 tacaaacggc agtcactcca gctatcgcta agttcggtgc tatcactggc atcattcatg 6960 gcgctggcgt tttagctgac caattcattg agcaaaaaac actgagcgat tttgagtctg 7020 tatacagtac taaaatcgat ggtttgttat cactgttatc agtaactgaa gcaagcaaca 7080 tcaagcaatt ggtattgttc tcgtcagcgg ctggtttcta cggtaaccct ggccaatctg 7140 attactcgat tgccaatgaa atcttaaaca agacggcata ccgctttaaa tcattgcacc 7200 cacaagctca agtcttgagt tttaactggg gtccttggga cggtggcatg gtaacgcctg 7260 agctaaaacg tatgtttgat cagcgtggtg tttacattat tccacttgat gcaggtgcgc 7320 agttgttact taatgaatta gccgctaatg ataatcgttg tccacaaatc ctagtgggta 7380 atgacttatc taaagatgct gctgatgaaa agagtactgc tgtaaaaaag ccacaagtta 7440 gtcgtttatc agatgcttta gtaactaaaa gcatcaaagc gactaacagt gactctttga 7500 acgatcattt tcaagtgaac gacaaccgct ttttagctga ccacatgatc aaaggcaatc 7560 aggtattacc aacggtatgc gcgattgctt ggatgagtga tgcagcacaa gcaacttata 7620 gtaaccgaga ctgtgcattg aaatatgtcg gtttcgaaga ctacaaattg tttaaagggg 7680 tagtttttga tggtaatgag gtggcagatt atcaaatcca attgtcgcct gtggccgctg 7740 tttcagagca tgaaacagta gtccgtattg ccgcaaaagt gtttagcctg aaaagcgatg 7800 gtaagccagt atttcattat gccgcaacaa tattgttagc ttctcagcct cttgatgcgg 7860 taacgctgca attaccggta ctagctgtag atattaacaa tttggttagc gatgaagcac 7920 aagcgttata cagcaatggc accttgttcc acggtgaaag tctgcagggt attaagcaga 7980 tattaagttg tgacgacaat ggcctgctat tggcttgtca gataacagat gttgcagcag 8040 ctaagcaggg tgcattcccc gtagctgcca acaatatctt tgctaatgat ttggtttatc 8100 aggcgatgtt ggtctgggtg cgcaagcaat ttggtttagg tagcttacct tcggtaacag 8160 ctgcttggac tgtgtatcgt gaagtggctg tcgatgaagt gttttatctg caactcaatg 8220 ttgttgaaca cgatctattg ggttctcgcg gcagtaaagc ccgttgtgat attcaattga 8280 ttgctgcaga tatgcagata cttgcagaag ttaaatcagc gcaagttagt gtcagtgaca 8340 ttttgaacga tatgtcttga gctagtcagt aacaaaaaat tatacgatag gcgtcatggt 8400 tagcatggcg tctatttttc ttcattgctt agcaagaaca acacaatatt aataactaaa 8460 agctattgat ttaaaaagta aaacagcgtt tttagttatt actattcgaa ataggatatt 8520 aaagagaata tgacggaatt agctgttatt ggtatggatg ctaaatttag cggacaggac 8580 aatattgatc gtgtggaacg tgctttatat caaggtgctt atgtagataa tgttagccgc 8640 gttagtatcg actctaacgt tattagctat ggcgaagaac aagttattac tgccgcgaca 8700 gtacttaact ctgtcggtct actagcgcaa acaaatcagt taaacatggc gaatatagcg 8760 gtattgctta ttgctgatat gagcagtgat gttgataacc agcttatagc gcaaattgca 8820 gcagaaattg aaaaacagtg tgcgtgttgt attgttattg ctgatttagg ccaagcatta 8880 aagcaagtaa cagatttggt taataaccaa gactgtcctg tggctgtgat tggtatgaat 8940 aacctagttc atgcgcctag ccatgatatt gaagctgcgg caacagccac tgcaacaata 9000 agctttgatg aaaccttcat tggttatagc agtgtagctg ggttcgctag tttacttatc 9060 gcttcaacaa cgtttgccaa tgctaagcag tgttatatat acgccaacat taagggcttt 9120 gctcagtctg gtgtcagtgc tcaatttaac gctgcaaaca ttaccgatac tgcaaacatt 9180 gcattacagc aagctggcat gaatgcagag caggttggtt tgttagaagt ttcagcagtc 9240 gctgattcgg caattgcatt gtctgaaagc caaggtttaa tgtctgctta taaccataca 9300 caaactttac ataccgcatt aagtagtgcc cgtagtgtga ctggcgaagg cggttgcttc 9360 tcgcaggttg cagggttatt gaaatgtgtg atcagtttgc atcagcgtta tattccggcg 9420 attaaagatt ggcaacagcc gagtgatacg caaatgtcac aatggcttca ctcaccattt 9480 tatatgcctg ttgatgctcg accttggttt ccacatgctg atggctctgc acacgttgcg 9540 gcttatagtt gtgtatctgc taacagctat tgtcacattc ttttacaaga aaatgactta 9600 actgaaagca agcttcaaac acttgcacaa aaccatccag tagctgatct gcgcactaat 9660 ggttactttg catcgagcga gttagcacta gttatagtgc aaggtaataa cgaagctcaa 9720 ttacgcagtg aattagagac tattgctggg cagttaagta ctactggtat aaatactgcc 9780 gatattaaac agatcgcggc tgattgttat gcccgaaatg atacggacaa agcctatagc 9840 gcagtactta ttgccgatac tgctgaagag ttaagcaaag agatcacttt agcgtttact 9900 ggtattgcta gcgtgtttaa tgacgatgct aaagaatgga aaaccccgaa gggtagttac 9960 tttactgcgt cgccagctaa caaagacgct gcaaacccag attcaaataa cagcacacag 10020 aatggcgtca cctttatgta cccaggtatt ggcgctacgt atgttggttt agggcgtgat 10080 ctatttcatc tattcccaca gatttatcag cctgtagcgg ctttagccga tgacattgct 10140 gccactctaa aagacacctt acttaatcca cgcagcatta gccgttatag ctttaaagaa 10200 ctcaagcagc tggatctgga tctacgcggt aatttagcta atatcgctga agccggtgtg 10260 ggttttgctt gcgtatttac caaggtattt gaagaagtat ttgccgttaa agctgacttt 10320 gctacaggtt atagcatggg tgaagtaagc atgtatgcgg cactaggctg ctggcaacaa 10380 ccgggattga tgagtgctcg tcttgcacaa tcgaatacct ttaatcatca actttgcggc 10440 gagttaagaa cactacgtca gcattggggg atggatgatg ttgctaacgg tacgttcgag 10500 cagatctggg aaacgtatac cattaaggca acgattgaac aggttgaaat tgcctctgca 10560 gatgaagacc gtgtgtattg caccattatc aatacgcctg atagcttact gttagcgggt 10620 tatccagaag cgtgtcagcg agtcattaag aatttaggtg tgcgcgcaat ggcattaaac 10680 atggcgaacg cgattcacag cgcgccagct tatgccgaat acgatcatat ggttgagtta 10740 taccatatgg acgttacccc gcgtattaat accaagatgt attcaagctc atgttattta 10800 cctattccac aacgcagcaa agcgatttca cacagtattg cgaaatgttt gtgtgatgta 10860 gtggatttcc cacgtttggt taatacctta catgacaaag gtgcgcgagt attcattgaa 10920 atgggtccag gtcgttcgtt atgtagctgg gtagataaga tcctagttaa taacgatggc 10980 gataatcaaa agcaaagcca acatgtatct gtgcctgtga atgccaaagg caccagtgat 11040 gaacttactt atattcgtgc gattgctaag ttaattagtc atggcgtgaa tttgaattta 11100 gatagcttgt ttaacgggtc aatcctggtt aaagcaggcc gtatagaaaa tacaaataaa 11160 tagtcaacat cgatatctag cgctggtgag ttatacctca tcagttgaaa tatggattta 11220 aagagagtaa ttatggaaaa tattgcagta gtaggtattg ctaatttgtt cccgggctca 11280 caagcaccgg atcaattttg gcagcaattg cttgaacaac aagattgccg cagtaaggcg 11340 accgctgttc aaatgggcgt tgatcctgct aaatataccg ctaacaaagg tgatacagat 11400 aagttttact gtgtgcacgg cggttacatc agtgatttca attttgatgc ttcaggttat 11460 caactcgata atgattattt agccggttta gatgacctta atcagtgggg gctttatgta 11520 acgaaacaag cgcttaccga tgcaggttat tggggcagta ctgcattaga aaactgtggt 11580 gtgattttag gtaatttgtc attcccaacc aagtcatcta atcagctgtt tatgcctttg 11640 tatcatcaag ttgttgataa tgcattaaag tcggtattac atcctgattt tcaactatca 11700 cattacacgg caccgaaaaa aatacatgcc gataatgcat tagtagctgg ttatccagct 11760 gcattgatcg cgcaagctgc gggtcttggt gggtcgcact ttgcactgga tgcggcttgt 11820 gcttcatctt gctatagcgt taagttagcg tgtgattacc tgcatacggg taaagccgac 11880 atgatgcttg ctggtgcggt atctgcagca gatccaatgt tcgttaatat gggtttctcg 11940 atattccaag cttatccagc taacaatgtt catgccccgt ttgaccaaaa ctcacaaggt 12000 ctatttgccg gtgaaggcgc gggcatgatg gtattgaaac gtcaaagcga tgcagtacgc 12060 gatggtgatc atatttacgc cattatcaaa ggcggcgcat tatcgaatga cggtaaaggc 12120 gagtttgtat taagcccgaa caccaagggc caagtattag tatatgaacg tgcttatgcc 12180 gatgctgatg ttgatccgag taccgttgat tatatcgaat gtcatgcaac gggcacacct 12240 aaaggtgaca atgttgaatt gcgttcgatg gaaacctttt tcagtcgcgt aaataacaaa 12300 ccattactgg gttcggttaa atctaacctt ggtcatttgt taacagccgc tggtatgccg 12360 ggcatgacga aagctatgtt agcgttaggt aaaggtctta ttcctgcaac gattaattta 12420 aagcaaccgc tgcaatctaa aaacggttac tttactggcg agcaaatgcc aacgacgact 12480 gtgccttggc caacaacgcc gggtgccaag acagataaac cgcgtaccgc aggtgtgagt 12540 gtatttggtt ttggtggcag taacgctcat ttggtattac aacagccaac gcaaacactc 12600 gagactaatt ttagtgttgc taaaccacgt gagtcattag ctattattgg tatggacagc 12660 cattttggta gtgccagtaa tttagcgcag ttcaaaacct tattaaataa taatcagaat 12720 accttccgtg aattaccaga acaacgctgg aaaggcatgg aaagtaacgc taatgtcatg 12780 cagtcgttac aattacgcaa agcgcctaag ggtagttacg ttgaacagct tgatattgat 12840 ttcttgcgtt tcaaagtacc gcctaatgaa aaagattgct tgatcccgca acaactaatg 12900 atgatgcaag tggcagacaa tgctgcgaaa gacgcaggtt tagtagaagg tcgtaatgtt 12960 gctgtattag tggctatggg catggaactg gaattacatc agtatcgtgg tcgcgttaat 13020 ctaacgacgc aaattgaaga cagcttatta cagcagggta ttaacctgac ggctgagcaa 13080 cgtgaagaac taaccaatat tgctaaagac ggtgtcgctt cggctgccca gctaaatcag 13140 tatactagct tcattggtaa tatcatggcg tcacgtattt cggcgttatg ggatttctct 13200 ggtccagcta ttactgtttc tgctgaagaa aactcagttt atcgttgtgt tgaattagct 13260 gaaaacctat ttcaaaccag tgatgttgaa gccgttatta ttgctgctgt tgatttgtca 13320 ggttcaattg aaaacattac cttacgtcag cactacggtc cagttaatga aaagggtcct 13380 gtaagtgaat gtggtcccgt aagtgaaaat ggctcggtaa acggaagtgg ctcagtaacc 13440 aacaatattc ttgatcagca acaatggctg gtgggtgaag gcgcagcggc tattgtcgtt 13500 aaaccgtcat cacaagtaac tgccgagcaa gtctatgcgc gtattgatgc agttagtttt 13560 gcccctggta gtgatgctaa agcaattacg attgcggcgg acaaagcatt aacattagcc 13620 ggtatcagtg cggcggatgt agcgagtgtt gaagcacatg caagtggttt tagtgccgaa 13680 aataatgctg aaaaaaccgc gttaccgact ttatacccaa gtgcaaacat cagttcggtg 13740 aaagccaata ttggtcatac gtttaatgca tcgggtatgg cgagtattat taaaactgcc 13800 ttgctgttag atcagaacac gagtcaagat cagaaaagcc aacatattgc tattaacggc 13860 ctaggtcgtg ataacagctg tgcgcatctt atcttatcga gttctgaaca agcacatcaa 13920 gctgcaccag cgcctgtatc tggtatggcc aagcaacgcc cacaattagt taaaaccatc 13980 aaactaggtg gtcaactaat tagcaatgcg attgttaaca gtgcgagttc atctttggat 14040 gcgattaaag cgcagtttgc tggtaagcat ttaaacaaag ttaaccagcc agtaatgatg 14100 gataacctga aaccacaagg cattcgcgct cattcaatga atgaacatgt ggtgacgaga 14160 ccttctagta ctcaagtgtc tcatacccaa gcgttcactc aagcgtctat tcaagcatct 14220 attcaagaac aagagctagc acaagagcaa gtacaaaata tgcaagctac agcagccgct 14280 gtaagttcac ccctttctca acatcaacat acaacgcagc ccgtagcggc accgagcgtt 14340 gttggagtga ctgtgaaaca taaagcaagt aaccaaattc atcagcaagc gtctacgcat 14400 aaagcatttt tagaaagtcg tttagctgca cagaaaaacc tatcgcaact tgttgaactg 14460 caaaccaagc tatcaacaca aactgctagt gacaatacgt ctaacaatac atctagcaat 14520 acagtgttaa caaatcctgt atcagcaact gcaacgccat taacactcgt atctactctt 14580 gttacaaata agcccgtagt cgtgacaagc ctaaccagca ctgaagcaaa agcgcaagca 14640 gcagctacac aagctggttt tcagataaaa ggacctgttg gttacaacta tccaccactg 14700 cagttaattg agcgttataa caaacctgaa aaagtgattt acgatcaagc tgatttggtt 14760 gaattcgctg aaggtgatat tggtaaggta tttggtgctg aatacaatat tattgatggc 14820 tactcgcgtc gtgtacgtct accaacctca gattatctat tagtaacacg tgttactgaa 14880 cttgatgcca aggtgcatga atacaagaaa tcatacatgt gcactgaata tgatgtacct 14940 gttgatgcac cgttcttaat cgatggtcag atcccttggt ctgttgcagt agaatcaggc 15000 cagtgtgatt taatgttgat ttcatacatc ggtattgatt tccaagctaa aggcgagcgt 15060 gtttaccgtt tacttgattg tgaattaacg ttccttgaag agatggcttt cggtggcgat 15120 actttacgtt acgagatcca cattgattcg tatgcacgta acggcgagca attattattc 15180 ttcttccatt acgattgtta cgtaggggat aagaaggtac ttatcatgcg taatggttgt 15240 gctggtttct ttactgacga agaactttct gacggtaaag gcgttattca taacgacaaa 15300 gacaaagctg aatttagcaa tgctgttaaa tcatcattca cgccgttatt acaacataac 15360 cgtggtcaat acgattataa cgacatgatg aagctggtta atggcgacgt ggcgagttgt 15420 tttggtccgc aatatgatca aggtggccgt aatccatcgt tgaaattctc gtctgagaag 15480 ttcttgatga ttgaacgtat taccaagata gatccaaccg gtggtcattg gggacttggc 15540 ctgttagaag gtcagaaaga tttagaccct gagcattggt atttcccttg tcactttaaa 15600 ggtgatcaag taatggctgg ttcgttgatg tcggaaggtt gtggccaaat ggcgatgttc 15660 ttcatgctgt ctcttggtat gcatactaat gtgaacaacg ctcgtttcca accgctacca 15720 ggtgaatcac aaacggtacg ttgtcgtggg caagtactgc cacagcgcaa taccttaact 15780 taccgtatgg aagtaacggc gatgggtatg catccacagc cattcatgaa agctaatatt 15840 gatattttgc ttgatggtaa agtggttgtt gatttcaaaa acttgagcgt gatgattagc 15900 gaacaagatg aacattcaga ttaccctgta acactgccga gtaatgtgca gcttaaaaca 15960 atttcgtctg ctgctgtaac gcctgttgct gtaactacga cgcctgttgc tttagctaca 16020 acgaacaatg ccgatctgga tgaacgtggt gttgaaccgt ttaagtatcc tgaacgtccg 16080 ttaatgcgcg tagagtcaga cttgtctgca ccgaaaagca aaggtgtgac accgattaag 16140 cattttgaag cgcctgttgt tgctggccac catagagtgc ctaaccaagc accgtttaca 16200 ccttggcata tgtttgagtt tgcgacgggt aatatctcta actgttttgg tcctgacttt 16260 gatgtttatg aaggtcgtat tccaccacgt acgccttgtg gcgatttaca agttgttact 16320 caggttgtgg aagtgcaggg cgaacgtctt gatcttaaaa atccatcaag ctgtatagct 16380 gaatactatg ttcctgaaga tgcttggtac tttactaaaa acagccatga aaactggatg 16440 ccatattcat taatcatgga aattgcattg caaccaaatg gctttatttc tggttacatg 16500 ggcacgacgc ttaaatatcc tgaaaaagat ctgttcttcc gtaaccttga tggtagcggc 16560 acgttattaa agcagattga tttacgcggc aaaacgattg tgaataaatc agtattagtt 16620 agtacggcta ttgctggtgg cgcgattatt caaagcttca cgtttgatat gtctgtagat 16680 ggcgagctat tttatactgg taaagcggta tttggttact ttagtggtga atcgctgact 16740 aaccaactgg gcattgataa cggtaagacg actaatgctt ggtttgttga taacaatacg 16800 cctgcagcta atattgatgt gttcgattta acggatcagt ctttagcgtt atacaaagcg 16860 cctgtaggta agccgcatta taaattggct ggtggccaga tgaacttcat cgatactgtg 16920 tctgttgttg aaggcggcgg taaagcggat gtggcttatg tttatggcga acgtacgatt 16980 gatgctgatg attggttctt ccgttatcac ttccaccaag atccggtgat gcctggttcg 17040 ttaggtgttg aagctattat tgagctgatg caaacctatg cgcttaaaaa tgacttgggt 17100 gcacagttta ctagtcctcg ttttgttgcg ccgatgacga aagttgattg gaaatatcgt 17160 ggtcaaatta cgccgttgaa taagcagatg tctctggacg tgcatattac cgagatcatt 17220 actgaatcag gtgaagtaag actcgttggt gatgcgaacc tgtctaaaga tggtctgcgt 17280 atttatgaag tgaagaatat tgttttaagt attgttgaag cgtaaaggct cgtgactaaa 17340 cgccgaattg gttaaagacg ccttgcacgc cgtgaatcca tccatggagg cttgaggctg 17400 gcatccatgc cagcaacagc aagcttactt taatcaatac ggcttagtag attatgcgat 17460 cgcattaggt cataaatgaa attgttcggg tgctgaactg aaatatttgg ttaacaacat 17520 aaaagttaaa aacaaatgtt tagttaatag ttcagtagtt acaaaaaagg aattaagaat 17580 gtcgagttta ggttttaaca atagcaatgc catcaattgg gcttggaaag tagatccagc 17640 gtcagttcat acacaggatg ttggcattaa agcagcttta atggatctga ctaatccgct 17700 ttacgtggct aataacgcag gtgttacagg tataacgaat catacgtcag tcgcgggtgc 17760 tattagcaat aatgttgacg ttgatgtact ggcgtttgca caaaagctaa cacctgaaga 17820 tctaggtgat gatgcgtaca agaaacaaca tggggttaaa tatgcatatc atggcggcgc 17880 aatggctaac ggtatcgcgt cggttgaact tgttgtcgca ctaggtaaag cagggttgtt 17940 atgttcgttc ggcgcggcag gtttagtacc tgacgcggtt gaagatgcta ttcgtcgtat 18000 tcaagctgaa ttaccaaatg gcccttacgc ggttaacttg atccatgcac cagcagaaga 18060 agcattagag cgtggggctg ttgaacgttt ccttaaactg ggtgttaaga cggtagaggc 18120 ttcagcttat cttggtttaa ctgagcacat tgtttggtat cgtgttgctg gtttatctaa 18180 aaacagtgat ggctcggtta atattggtaa caaggttatc gctaaagtat cgcgtactga 18240 agttggtcgc cgctttatgg aaccagcacc gcaaaagtta ctggataagt tattagcaca 18300 aaacaagatc acccctgaac aagctgctct agcgctgctt gtacctatgg ctgatgacat 18360 tactgctgaa gctgattctg gtggccatac tgacaaccgt ccatttttaa ctttattacc 18420 gactatcatc ggactgcgtg atgaagtgca agcgaagtat aacttctctc ctgcattacg 18480 tgttggtgct ggtggcggta tcggaacgcc tgaagcagca ctcgctgcat ttaacatggg 18540 cgctgcttat atcgttctgg gttctgtgaa tcaggcgtgt gttgaagcgg gtgcatctga 18600 atatacgcgt aaactgttat cgacagttga aatggccgat gtgactatgg cacctgctgc 18660 tgatatgttt gaaatgggcg taaagctaca agtattaaaa cgtggttcta tgttcgctat 18720 gcgtgcgaag aaactgtatg acttgtatgt gacttatgac tcgattgaag atatcccagc 18780 tgcggaacgt gagaagattg aaaaacaaat cttccgtgca aacctagacg agatctggga 18840 tggcactatc gctttcttca ctgaacgtga tccagaaatg ctagcccgtg caatgagcag 18900 ccctaaacgt aaaatggcgc ttatcttccg ttggtatctt ggtctttctt cacgctggtc 18960 aaacaccggt gagaagggac gtgaaatgga ttatcaaatt tgggctggcc caagtctagg 19020 cgcatttaac agctgggtga aaggttctta ccttgaagac tatacccgcc gcggcgcagt 19080 tgatgttgct ttgcatatgc ttaaaggtgc tgcgtactta caacgtgtga accagttgaa 19140 gctgcaaggc gttagcttaa gtacagaatt agcaagttat cgtactagtg attaattttt 19200 attaatacat ttgatatatt gaagttataa cggagggcta gtctttccgt tataacttga 19260 ttcactcttg gatatagtgg ataaatatgt tgcagtactt tacgctaatc gtaaattcat 19320 ttttgtgggc gttatgtgct gaaatggaat atactttaga ttgaaaagtg atagtttgta 19380 atgtagcatc aacagtaata gttagtttct ctcaggatcg cgagtcatga aaaatcaaaa 19440 gataaaagta actcaaaaaa tgatatatag agcagttgct agttctacag caattgaaac 19500 aagagcttct gtaaattcga ttgaaaaaaa gcttaaagct aacaagcata aatacagttc 19560 actatcacta gcctgctagt atatctctaa ctaatctctc catatgctgg tagtcgtgtg 19620 atacaccagc ttgaatggac gctatataaa aatctttatt tttatcccaa agactatagt 19680 ccaataagcc taaccctgct ttgttgcaga tacagtccat aagtaatcga ctcatacgac 19740 catttccttc acgaaaaggg tgtattaaga taaattcaat atggctgcga gctaagtatg 19800 ctactaactc ttcatgattc atatacggca aatttttaaa ttgggataag tattcgcttt 19860 caaatagatc tatacatcga ccaatttgaa gtggtgaagc aaactgaaat ccacctttcc 19920 ccatatcaac gttcctaatt tttccagccc attcatagac gttgccaagc cattgcctat 19980 gccaggttga aatgttttca aaagaaaaat tatcagactc cggaatgctg gtggtaaaac 20040 actgttcgta tagttttgtt aacagtattg cttcaacatc atccatttca gttaggtcaa 20100 taatatcaag cttgtttacc atgactgtat tgttagaccc tagctgatat tgagtttcag 20160 aagcgtcgat ttcgtattta gtcataactg ggtgacatat ttgggaaccg taatatgagt 20220 ttaaagcaca taccaaacaa ttcaacttaa ttttgttcag catgcgcgtg aagaaacttt 20280 ttgacttgga cttaagttaa gacattccag ctgcggagcg tgatccagaa atgctagccc 20340 gtgcaatgag tagccctgaa cgtaaaattg cacttatctt ccgttgtatc ttggtctttc 20400 ttcacgctgg tcaagcatag gcgagaaggg acgtgaaatg gattgtcaga tttgggctgg 20460 cccaagttta ggcgcattca acagctgggt taaaggttct taccttgaag actatacccg 20520 ccgcggcgct gtagatgttg ctttgcatat gcttaaaggt gcagcgtatt tacaacgtgt 20580 aaaccagttg aaactgcaag gcgttagctt aagtacagaa ttggcaagtt atcgtactag 20640 ttttatatgc aatttattga agtagcaata gagtttaaat aaggtttttt aatctttaaa 20700 gtacacgtga tataaacatt catatagtat aaagtgttag attaaataac cactaagaat 20760 atgttttaat tacatttttt aacgttactg atgataaata agttttaaac atacctcttt 20820 taattcataa ttataatctg cacttagtca tactacttta tttaccaatt ggattattta 20880 tggatttcat cgaacgttta caaggcttat caaaaaaact ttcacaagtt tcaggaacac 20940 tagaaacaga agaagctaca aagaatgctc ttattatgcc atttttgcac actgtactag 21000 gttatgatgt gtttgatcct agtgaagttg tacctgagtt tactgcggat actggtacaa 21060 aaaaaggtga aaaagtagat tatgcattaa tgaaggatgg agaagttcag atccttattg 21120 agtgtaaaaa attaagtgaa aaactatcaa caaaacatgc tagtcaatta ttccgatatt 21180 tctctgttac gaatgcaaga gttgctattc taacaaatgg tcgtgtttat gagttttata 21240 cagatttaga tgcaccgaat aagatggatg aaaaaccatt tttaacatta gaccttgctg 21300 aacttgatga acacatcgtt cctgaaatta agaaattaac taaatcttca ttcgatgttg 21360 attctattgt agatgctgct ggagagctta agtatcttag ccaaattaaa aaggtcttat 21420 ctgaacaatt taagcagcct gaagaagaat ttgttaagtt ttttacttca cgtgtttatg 21480 agggtgttca aacatctaag gttaaagcac aatttctaga tatcacttca aaggctctta 21540 aacaattttt gagcgatagt atcaatgata gattgaagtt tgcacttggc gatagtaaca 21600 atgaacctgt taatgttgta tctaatattt ctgagattaa agttgaagaa atcgttgatg 21660 attctgaaaa agaagtatca aaaattgtaa caactgagtg tgagcttgac ggttttaata 21720 ttgtaaaagc tatattaaga gcaaaggttg atgtctctcg tataacttac agggatacac 21780 aaagctactt tggtgtgtta cttgatgata ataaccgtaa gccattatgt agattacact 21840 ttaatgccaa gcaaaaatat cttggtgttg ttggtgaaga taagaaagag actcgctacc 21900 caatagaaag tttggatgaa ttgtataatc atgcagactt attattaaat accatatcac 21960 aatacgaata attataggaa ctaaaaaggg aattaaaaat tccctttttc atatcatgaa 22020 tatattcttc aatcgaaata ccacaacttt aataatcatt ttctctgttg taattgcatt 22080 tttaagtagc tttaatcaat caatgttcat tcatatttta aactggatgg taatttctta 22140 ttttacattt gaattagttt ataaaataaa aatattgtca tggaaaagct atatatcgtc 22200 gttagggcat aaatttgatt tttttatatt aattatcagc attgccatgc tcgctgctcc 22260 tactgcagaa ttaggtagct tagtatatct ccgagtattt agagttttat cgttatttcg 22320 ggttattaag ttaatcccta atgccgagca cattgttaga ggtgtatcta gagctgtaaa 22380 agcatctaag acagtaatga tattacttgg tgttatgctt gtgttttttt cgatgcttgg 22440 atatacctta ttttcagcat acttacctga gttttttggc gatccgctaa aatcaatgaa 22500 taccgtattt actattttca ctgttgaaaa ttggggtgct gttcctgacg cggctaaaaa 22560 aattgggact gactatatgt attacgcagt taattcattt gttatcactg tattaatatt 22620 aggtgggttt atagcgttat cattagctaa tgcagttttc gttgatgaaa tggcaagcga 22680 taataatgat gatttaaagc ttgaactaga aaaaataaga caagagaata tagaagtaaa 22740 atcaattctt atggaaatga ataaaaagat taataaattg agtgacgttg ataaactggt 22800 aatgcataaa ttaagagagc aacactcttt ccctgaaata ggtaataagc caacacaaaa 22860 aactaatgtc agttctaaat aagtcaataa accaaacagg ctagcagcat ttagaggtgt 22920 ataaaaacaa tcgcatgggt agtgtacctg tgagtcccta cctatatctt atctcatccc 22980 tattgataaa aagggtgatg tagccaattg ttatgaccct gagcggctca aaaagattga 23040 atccgcagtt gctaaaggtg actataatgc accattgcgt catgcctgtt aatgcatgac 23100 gcaatggtgc ttcgcatcta ttaacaattt ctctaaagtg tacacatctg atggcattaa 23160 tggcgcggga ttaccagcgg ataactttat agtgactaat cttactatcc catccatatt 23220 ccccattgat aagctatcat caactgatag cggtgtttat ttatatcaac catcgtttcg 23280 tttctttaaa gataaaaacc tatctaatca attgaaaagc ggcaagttag atgactttaa 23340 agaagaaggg gtgtttagga ttgctccatt aagtaatgat gaagtgcttt gtatatgagg 23400 actctgctag cagtcaatgt aatagttctt cataacggtt aaaccaagta cagcatcaac 23460 ggtagcgatt gataccttaa taagttttag cgcacaaaat agaactattt aaagatctca 23520 acaaggcgat gatttgataa tgaattattg ccttgttact atcaagagac tttaactgac 23580 tgagatagaa agatatgaca cggaagaaca tattgatcac aggcgcaagt tcagggttgg 23640 gccgaggtat ggccatcgaa tttgcaaaat caggtcataa cttagcactg tgtgcacgta 23700 gacttgataa tttaattgca ctgaaagcag aactcttagc cctcaatcct cacatccaaa 23760 tcgaaatcaa acctcttgat gtcaacgaac atgaacaagt cttcactgtt ttccatgaat 23820 t 23821<210> 1 <211> 23821 <212> DNA <213> Shewanella sp. SCRC-21406 (FERM BP-5979) <400> 1 gtgagatatc agactaattt tagcttatta atttgttagt tgtttatatt taataaagac 60 gtaatttaac ttaaatgcaa ttaattagg cgtaaatagattactag attattg cgtaaatagattacattagcattachtattcgg ataaagttta atctgttatt ttagcgttta cctggtctaa 180 tcagtgaggt ttttagccat tattggtggg attgaagtga ttttcaaagc tatgtatatt 240 attgcaaata taaattgtaa caattaagac tttgaacact tgagttcaat ttcaaattga 300 ttggcataaa atttaaaaca gctaaatcta cctcaatcat tttagcaaat gtatgcaggt 360 agattttttt cgccatttaa gagtacactt gtacgctagg tttttattta gtgtgcaaat 420 gaacgttttg atgagcattg tttttagtgc acaaaataga tccttacagg agcaataacg 480 caatggctaa aaagaacacc acatcgatta agcacgccaa ggatgtgtta agtagtgatg 540 atcaacagtt aaattctcgc ttgcaagaat gtccgattgc catcattggt atggcatcgg 600 tttttgcaga tgctaaaaac ttggatcaat tctgggataa catcgttgac tctgtggacg 660 ctattattga tgtgcctagc gatcgctgga acattgacga ccattactcg gcagataaaa 720 aagcagcgatcagcatag catcagcat gag tttgatc 780 cgatggaatt tggtttaccg ccaaatatcc tcgagttaac tgacatcgct caactgttgt 840 cattaattgt tgctcgtgat gtattaagtg atgctggcat tggtagtgat tatgaccatg 900 ataaaattgg tatcacgctg ggtgtcggtg gtggtcagaa acaaatttcg ccattaacgt 960 cgcgcctaca aggtccggta ttagaaaaag tattaaaagc ttcaggcatt gatgaagatg 1020 atcgcgctat gatcatcgac aaatttaaaa aggcctacat cggctgggaa gagaactcat 1080 tcccgggcat gctaggtaac gtcattgctg gccgtatcgc caatcgtttt gattttggtg 1140 gtactaactg tgtggttgat gcggcatgtg ccggctccct tgcagcagta aaaatggcga 1200 tttcggactt acttgaatat cgctctgaag taatgatctc gggtggcgta tgttgtgata 1260 actcgccatt catgtatatg tcattctcaa aaacaccggc atttaccacc aacgatgata 1320 tccgtccgtt tgatgacgat tcaaaaggca tgctcgttgg tgaaggtatc ggcatgatgg 1380 cgtttaaacg ccttgaagat gctgaacgtg acggcgacaa aatttattcg gtactgaaag 1440 gtatcggtac atcttcagat ggtcgtttca aatctattta tgctccgcgc ccagatggcc 1500 aagcaaaggc gctgaaacgt gcttatgaag atgcaggttt tgcccctgaa acttgtggac 1560 taattgaagg ccatggtaca ggtactaaag ccggtgatgc agcagaattt gctggtctaa 16 20 ctaaacactt tggcgctgcc agtgatgaaa aacaacatat cgcattaggc tcagttaaat 1680 cgcaaattgg tcatacaaaa tctgcggctg gctctgcggg tatgattaag gcggcattag 1740 ccctgcatca taaaatccta ccagcaacaa ttcatatcga taagccaagc gaagccttgg 1800 atatcaaaaa cagcccgtta tacctaaaca gcgaaacgcg tccttggatg ccacgtgaag 1860 atggtattcc acgtcgtgct ggtatcagct catttggttt tggtggtacg aacttccata 1920 ttattttaga agagtaccgt ccaggtcacg atagtgcata tcgcttaaat tcagtgagtc 1980 aaactgtgtt gatctcggca aacgatcaac aaggtatcat tgcagaatta aataactggc 2040 gtactaaact ggctgtcgat gccgaacagc aagcatttgt gtttaacgac ttagtgacaa 2100 cgtggccatt aaaaacacca tcagttaatc aagctcgttt aggttttgtt gcgcgtaatg 2160 ccaacgaagc gattgcgatg attgatacgg cattaaaaca atttaacgct aatgcagata 2220 aaaccacatg gtcagtgcct accggggttt actatcgcca agctggtatt gatgcaacag 2280 gtaaagtcgt ggcgctattc tcaggacaag gttcgcaata tgtgaacatg ggtcgagaat 2340 taacctgcaa cttcccaagc atgatggaca gtgctgcggc aatggataaa gaattcagcg 2400 gggctggctt aggtcagtta tctgccgtta ctttcccaat ccctgtttac acggataccg 2460 agc gtaagct acaagaagat caattacgtt taactcaaca tgcacaacca gcgattggta 2520 gtttgagtgt tggtctgttc aagactttcc aacaagcagg ctttaaagct gatttcgctg 2580 ccggtcatag tttcggtgaa ttaacggcat tatgggctgc tgatgtactg agcgaaagcg 2640 attacatgat gttagcgcgt agtcgtggtc aagcaatggc tgcacctgag caacaagatt 2700 ttgatgcggg taagatggcc gctgttgttg gcgatccaaa gcaagtagcc gtcatcattg 2760 atactattga tgatgtctct attgctaact acaactcgaa taaccaagtt gttattgcgg 2820 gtactactgc gcaggttgct gtagcagtta caaccttagg caatgctggt ttcaaagttg 2880 tgccactgcc ggtatccgct gcgttccata cacctttagt gggtcatgct caaaaaccat 2940 ttgctaaagc ggttgatcgc gctaaattta aagcgccaag catccctgta tttgctaatg 3000 gcacaggctt agttcattca agtaaaccga atgacattaa gaaaaacctg aaaaaccaca 3060 tgttagaatc tgttcatttc aatcaagaaa ttgacaacat ctatgctgat ggtggtcgcg 3120 tatttatcga atttggtccg aagaatgtat taactaaatt ggttgaaaac attctcactg 3180 acaaatctga tgtgactgct atcgcggtta atgctaatcc aaaacaacct gctgatgtac 3240 aaatgcgtca agctgcgctg caaatggcag tgcttggtgt cgcattagac aatattgacc 3300 cgtacgacg c agttaagcgc ccacttgttg cgccaaaagc atcgccaatg ttgatgaaat 3360 tatctgcagc gtcttatgtt agtccaaaaa cgaagaaagc gtttgcagat gcattaacgg 3420 atggttggac ggtgaaacaa gccaaagccg caccagttgt tgtgtcacaa ccacaagtga 3480 ttgaaaagat cgttgaagtt gaaaagatag tcgaacgcat tgtcgaagtt gagcgtatcg 3540 ttgaagtaga aaaaattgtc tacgttaatg ctgatggttc acatgcaacg acgaacaata 3600 gcgctattaa taatggcatg catagctctg acgccgatct cgttgcctca attgaacaga 3660 gtgttggtca gtttgttgca caccaacagc aattattaaa tgtacatgaa cagtttatgc 3720 aaggtccaca agactatgca aaaacagtgc aaaacgtact tgctgcgcaa accaataatg 3780 aattaccgga aagtttagac cgtacattgt ctatgtataa cgagttccaa tcagaaacgc 3840 tacgtgtaca tgaaacgtac ctgaacaatc agacgagcag catgaacact atgcttactg 3900 gtgctgaaac tgatgtgcta gtagctcctg tcactcaagt attgaataca cccgttgccg 3960 ctagtcacaa ggcagttgct ccagttattg ctaatacagt gacgaatgtt gtatctagtg 4020 tcagtaataa cgcggcggtt gcagtgcaaa ctgtggcatt agcgcctacg caagaaatcg 4080 ctccagcagt cgttactacg ccagcacccg cattggttgc tattgtggct gaacctgtga 4140 ttgttgcgcc tgtt gctaca gaagttgcac caattacatc atcagttaca cctgttgtcg 4200 caactcaagc ggctatcgat gtagcaacta ttaacaaagt aatgttagaa gttgttgctg 4260 ataaaaccgg ttatccaaca gacatgcttg aactgagcat ggacatggaa gctgatttag 4320 gtatcgactc aatcaaacgt gttgaaattc taggcgcagt acaggaattg atccctgact 4380 tacctgagct taaccctgaa gatcttgctg agctacgtac gcttggtgag attgtcgatt 4440 atatgaatgt taaagcgcaa gaggctgctc ctgtatcagc acctgcatct gctggtattg 4500 atttagccca catccaaaac gtaatgttag aagtggttgc agacaaaacc ggttacccaa 4560 ctgacatgct tgaactgagc atggacatgg aagctgactt aggtattgat tcaatcaaac 4620 gtgtggaaat cttaggcgca gtgcaggaga tcatcactga tttacctgag ctaaatcctg 4680 aagacttagc tgagctacgc accttaggtg aaatcgttag ttacatgcaa agcaaagcgc 4740 ccgtcgctga aagtgtgact gttacagatt cagacgttac ttctgtcgca agctctgcac 4800 catctatcga tttgaaccac attcaaacag tgatgatgga tgtagttgca gataagactg 4860 gttatccaac agatatgtta gaacttggca tggacatgga agctgactta ggtatcgatt 4920 caatcaaacg tgtggaaata ttaggcgcag tgcaggagat cattactgat ttacctgagc 4980 taaaccctga agatcttgct gaattacgca cgctaggtga aatcgttagc tacatgcaaa 5040 gcaaagcgcc tgtagctgaa gcgcctgcag ctgtagaaag tgcacctatt ggtgtaacaa 5100 gttcagcacc gtctatcgat ttaaaccaca tccaaactgt gatgatggaa gttgttgcag 5160 acaaaaccgg ttatccagta gacatgttag aacttgctat ggacatggaa gctgaccttg 5220 gtatcgactc aatcaaacgt gtagaaattc taggtgcggt acaggaaatc atcactgact 5280 tacctgagct aaaccctgaa gatcttgctg aactacgtac gttaggtgaa atcgttagtt 5340 acatgcaaag caaagtgccc gtagctgaag cgcctgttgc tgtagaaagt gcacatactg 5400 gtgtaacaag ttcagcaccg tctatcgatt tagaccatat ccaaaacgta atgatggatg 5460 ttgttgctga taagactggt tatcctgcca atatgcttga actagcaatg gacatggaag 5520 ctgaccttgg tattgattca atcaagcgtg ttgaaattct aggcgcggta caggagatca 5580 ttactgattt acctgaacta aacccagaag acttagcgga actacgtacg ttagaagaaa 5640 ttgtaactta catgcagagt aaagctcgtg gtgttactgc aaatgtagta gctaatcatg 5700 aacctgaaaa aagcgcagtg tcagatgcat ttatgcagaa cagcgttgct actatcacgg 5760 cagctgtagc gccaaaagca gagtttcaac cagcgccgag cgcaacggtt gctatctcgc 5820 gtctaagctc tatcagtaaa ataag ccaaa attgccaagg cgctaatgcc ttaatcgtgg 5880 ctgatggcac tgataatgca gtattactcg cagaccacct actgcaaact ggctggaatg 5940 taactgcatt gcaaccaact tgggtagcag taacaacgac gaaagcattt aatcaatcag 6000 taaacctggt gagcttaaat ggcgttgatg aaactgaaat caacaacatc attgctgcta 6060 acgcacaatt ggatgcagtt atctatctgc acgccagtag cgaaattaat gctattgaat 6120 acccacaagc atctaagcaa ggcctgatgt tagccttctt attagcgaaa ttgagtaaag 6180 taactcaagc cgctaaagtg cgtggttctt tcatggtagt tactcagcag ggcggttcac 6240 ttggttttag caatgacgca caagctgacc tagtacaggc agacttagta cagacagacc 6300 ttgtacaaag cggcttaaac ggtttagtta aaacactgtc acacgaatgg gataacgtat 6360 tctgtcgtgc ggtagatatg ccaacatcat tagctgcagc gcaagtagca actcttgttt 6420 gtgatgaact gtttgacgca gatagcgtgt taactgaagt aggttatcaa caagcagagc 6480 aaaatggtgt tgcccgcatt accttaactg gtgttgcgac tgacagttat gcactgactg 6540 ctggtaataa cattgatgct aattcggtat tcttagtgag tggtggcgca aaaggcgtaa 6600 cagcccattg cgtagtacgt atcgctgaag agtatcaagc taagttcatc ttattaggac 6660 gctcaaaatt ttcaaatgat gagccgagct gggcaaacgg tattactgaa gaagcggcat 6720 taaagaaagc agcaatgcag gctttaattg cagcaggtga taaaccaacg cccgttaaga 6780 tcgtacagtt aatcaaacca attcaagcta atcgtgaaat tgcgcaaact ttgtctgcaa 6840 ttaccgctgc tggtggccaa gctgaatatg tttctgcaga tgtaaccaat gcagcaagcg 6900 tacaaacggc agtcactcca gctatcgcta agttcggtgc tatcactggc atcattcatg 6960 gcgctggcgt tttagctgac caattcattg agcaaaaaac actgagcgat tttgagtctg 7020 tatacagtac taaaatcgat ggtttgttat cactgttatc agtaactgaa gcaagcaaca 7080 tcaagcaatt ggtattgttc tcgtcagcgg ctggtttcta cggtaaccct ggccaatctg 7140 attactcgat tgccaatgaa atcttaaaca agacggcata ccgctttaaa tcattgcacc 7200 cacaagctca agtcttgagt tttaactggg gtccttggga cggtggcatg gtaacgcctg 7260 agctaaaacg tatgtttgat cagcgtggtg tttacattat tccacttgat gcaggtgcgc 7320 agttgttact taatgaatta gccgctaatg ataatcgttg tccacaaatc ctagtgggta 7380 atgacttatc taaagatgct gctgatgaaa agagtactgc tgtaaaaaag ccacaagtta 7440 gtcgtttatc agatgcttta gtaactaaaa gcatcaaagc gactaacagt gactctttga 7500 acgatcattt tcaagtgaac gacaaccgct ttttag ctga ccacatgatc aaaggcaatc 7560 aggtattacc aacggtatgc gcgattgctt ggatgagtga tgcagcacaa gcaacttata 7620 gtaaccgaga ctgtgcattg aaatatgtcg gtttcgaaga ctacaaattg tttaaagggg 7680 tagtttttga tggtaatgag gtggcagatt atcaaatcca attgtcgcct gtggccgctg 7740 tttcagagca tgaaacagta gtccgtattg ccgcaaaagt gtttagcctg aaaagcgatg 7800 gtaagccagt atttcattat gccgcaacaa tattgttagc ttctcagcct cttgatgcgg 7860 taacgctgca attaccggta ctagctgtag atattaacaa tttggttagc gatgaagcac 7920 aagcgttata cagcaatggc accttgttcc acggtgaaag tctgcagggt attaagcaga 7980 tattaagttg tgacgacaat ggcctgctat tggcttgtca gataacagat gttgcagcag 8040 ctaagcaggg tgcattcccc gtagctgcca acaatatctt tgctaatgat ttggtttatc 8100 aggcgatgtt ggtctgggtg cgcaagcaat ttggtttagg tagcttacct tcggtaacag 8160 ctgcttggac tgtgtatcgt gaagtggctg tcgatgaagt gttttatctg caactcaatg 8220 ttgttgaaca cgatctattg ggttctcgcg gcagtaaagc ccgttgtgat attcaattga 8280 ttgctgcaga tatgcagata cttgcagaag ttaaatcagc gcaagttagt gtcagtgaca 8340 ttttgaacga tatgtcttga gctagtcagt aacaaaaaat t atacgatag gcgtcatggt 8400 tagcatggcg tctatttttc ttcattgctt agcaagaaca acacaatatt aataactaaa 8460 agctattgat ttaaaaagta aaacagcgtt tttagttatt actattcgaa ataggatatt 8520 aaagagaata tgacggaatt agctgttatt ggtatggatg ctaaatttag cggacaggac 8580 aatattgatc gtgtggaacg tgctttatat caaggtgctt atgtagataa tgttagccgc 8640 gttagtatcg actctaacgt tattagctat ggcgaagaac aagttattac tgccgcgaca 8700 gtacttaact ctgtcggtct actagcgcaa acaaatcagt taaacatggc gaatatagcg 8760 gtattgctta ttgctgatat gagcagtgat gttgataacc agcttatagc gcaaattgca 8820 gcagaaattg aaaaacagtg tgcgtgttgt attgttattg ctgatttagg ccaagcatta 8880 aagcaagtaa cagatttggt taataaccaa gactgtcctg tggctgtgat tggtatgaat 8940 aacctagttc atgcgcctag ccatgatatt gaagctgcgg caacagccac tgcaacaata 9000 agctttgatg aaaccttcat tggttatagc agtgtagctg ggttcgctag tttacttatc 9060 gcttcaacaa cgtttgccaa tgctaagcag tgttatatat acgccaacat taagggcttt 9120 gctcagtctg gtgtcagtgc tcaatttaac gctgcaaaca ttaccgatac tgcaaacatt 9180 gcattacagc aagctggcat gaatgcagag caggttggtt tgttaga agt ttcagcagtc 9240 gctgattcgg caattgcatt gtctgaaagc caaggtttaa tgtctgctta taaccataca 9300 caaactttac ataccgcatt aagtagtgcc cgtagtgtga ctggcgaagg cggttgcttc 9360 tcgcaggttg cagggttatt gaaatgtgtg atcagtttgc atcagcgtta tattccggcg 9420 attaaagatt ggcaacagcc gagtgatacg caaatgtcac aatggcttca ctcaccattt 9480 tatatgcctg ttgatgctcg accttggttt ccacatgctg atggctctgc acacgttgcg 9540 gcttatagtt gtgtatctgc taacagctat tgtcacattc ttttacaaga aaatgactta 9600 actgaaagca agcttcaaac acttgcacaa aaccatccag tagctgatct gcgcactaat 9660 ggttactttg catcgagcga gttagcacta gttatagtgc aaggtaataa cgaagctcaa 9720 ttacgcagtg aattagagac tattgctggg cagttaagta ctactggtat aaatactgcc 9780 gatattaaac agatcgcggc tgattgttat gcccgaaatg atacggacaa agcctatagc 9840 gcagtactta ttgccgatac tgctgaagag ttaagcaaag agatcacttt agcgtttact 9900 ggtattgcta gcgtgtttaa tgacgatgct aaagaatgga aaaccccgaa gggtagttac 9960 tttactgcgt cgccagctaa caaagacgct gcaaacccag attcaaataa cagcacacag 10020 aatggcgtca cctttatgta cccaggtatt ggcgctacgt atgttggttt a gggcgtgat 10080 ctatttcatc tattcccaca gatttatcag cctgtagcgg ctttagccga tgacattgct 10140 gccactctaa aagacacctt acttaatcca cgcagcatta gccgttatag ctttaaagaa 10200 ctcaagcagc tggatctgga tctacgcggt aatttagcta atatcgctga agccggtgtg 10260 ggttttgctt gcgtatttac caaggtattt gaagaagtat ttgccgttaa agctgacttt 10320 gctacaggtt atagcatggg tgaagtaagc atgtatgcgg cactaggctg ctggcaacaa 10380 ccgggattga tgagtgctcg tcttgcacaa tcgaatacct ttaatcatca actttgcggc 10440 gagttaagaa cactacgtca gcattggggg atggatgatg ttgctaacgg tacgttcgag 10500 cagatctggg aaacgtatac cattaaggca acgattgaac aggttgaaat tgcctctgca 10560 gatgaagacc gtgtgtattg caccattatc aatacgcctg atagcttact gttagcgggt 10620 tatccagaag cgtgtcagcg agtcattaag aatttaggtg tgcgcgcaat ggcattaaac 10680 atggcgaacg cgattcacag cgcgccagct tatgccgaat acgatcatat ggttgagtta 10740 taccatatgg acgttacccc gcgtattaat accaagatgt attcaagctc atgttattta 10800 cctattccac aacgcagcaa agcgatttca cacagtattg cgaaatgttt gtgtgatgta 10860 gtggatttcc cacgtttggt taatacctta catgacaaag gtgc gcgagt attcattgaa 10920 atgggtccag gtcgttcgtt atgtagctgg gtagataaga tcctagttaa taacgatggc 10980 gataatcaaa agcaaagcca acatgtatct gtgcctgtga atgccaaagg caccagtgat 11040 gaacttactt atattcgtgc gattgctaag ttaattagtc atggcgtgaa tttgaattta 11100 gatagcttgt ttaacgggtc aatcctggtt aaagcaggcc gtatagaaaa tacaaataaa 11160 tagtcaacat cgatatctag cgctggtgag ttatacctca tcagttgaaa tatggattta 11220 aagagagtaa ttatggaaaa tattgcagta gtaggtattg ctaatttgtt cccgggctca 11280 caagcaccgg atcaattttg gcagcaattg cttgaacaac aagattgccg cagtaaggcg 11340 accgctgttc aaatgggcgt tgatcctgct aaatataccg ctaacaaagg tgatacagat 11400 aagttttact gtgtgcacgg cggttacatc agtgatttca attttgatgc ttcaggttat 11460 caactcgata atgattattt agccggttta gatgacctta atcagtgggg gctttatgta 11520 acgaaacaag cgcttaccga tgcaggttat tggggcagta ctgcattaga aaactgtggt 11580 gtgattttag gtaatttgtc attcccaacc aagtcatcta atcagctgtt tatgcctttg 11640 tatcatcaag ttgttgataa tgcattaaag tcggtattac atcctgattt tcaactatca 11700 cattacacgg caccgaaaaa aatacatgcc gataatg cat tagtagctgg ttatccagct 11760 gcattgatcg cgcaagctgc gggtcttggt gggtcgcact ttgcactgga tgcggcttgt 11820 gcttcatctt gctatagcgt taagttagcg tgtgattacc tgcatacggg taaagccgac 11880 atgatgcttg ctggtgcggt atctgcagca gatccaatgt tcgttaatat gggtttctcg 11940 atattccaag cttatccagc taacaatgtt catgccccgt ttgaccaaaa ctcacaaggt 12000 ctatttgccg gtgaaggcgc gggcatgatg gtattgaaac gtcaaagcga tgcagtacgc 12060 gatggtgatc atatttacgc cattatcaaa ggcggcgcat tatcgaatga cggtaaaggc 12120 gagtttgtat taagcccgaa caccaagggc caagtattag tatatgaacg tgcttatgcc 12180 gatgctgatg ttgatccgag taccgttgat tatatcgaat gtcatgcaac gggcacacct 12240 aaaggtgaca atgttgaatt gcgttcgatg gaaacctttt tcagtcgcgt aaataacaaa 12300 ccattactgg gttcggttaa atctaacctt ggtcatttgt taacagccgc tggtatgccg 12360 ggcatgacga aagctatgtt agcgttaggt aaaggtctta ttcctgcaac gattaattta 12420 aagcaaccgc tgcaatctaa aaacggttac tttactggcg agcaaatgcc aacgacgact 12480 gtgccttggc caacaacgcc gggtgccaag acagataaac cgcgtaccgc aggtgtgagt 12540 gtatttggtt ttggtggcag taacgctcat ttggtattac aacagccaac gcaaacactc 12600 gagactaatt ttagtgttgc taaaccacgt gagtcattag ctattattgg tatggacagc 12660 cattttggta gtgccagtaa tttagcgcag ttcaaaacct tattaaataa taatcagaat 12720 accttccgtg aattaccaga acaacgctgg aaaggcatgg aaagtaacgc taatgtcatg 12780 cagtcgttac aattacgcaa agcgcctaag ggtagttacg ttgaacagct tgatattgat 12840 ttcttgcgtt tcaaagtacc gcctaatgaa aaagattgct tgatcccgca acaactaatg 12900 atgatgcaag tggcagacaa tgctgcgaaa gacgcaggtt tagtagaagg tcgtaatgtt 12960 gctgtattag tggctatggg catggaactg gaattacatc agtatcgtgg tcgcgttaat 13020 ctaacgacgc aaattgaaga cagcttatta cagcagggta ttaacctgac ggctgagcaa 13080 cgtgaagaac taaccaatat tgctaaagac ggtgtcgctt cggctgccca gctaaatcag 13140 tatactagct tcattggtaa tatcatggcg tcacgtattt cggcgttatg ggatttctct 13200 ggtccagcta ttactgtttc tgctgaagaa aactcagttt atcgttgtgt tgaattagct 13260 gaaaacctat ttcaaaccag tgatgttgaa gccgttatta ttgctgctgt tgatttgtca 13320 ggttcaattg aaaacattac cttacgtcag cactacggtc cagttaatga aaagggtcct 13380 gtaagtgaat gtggtcccgt aa gtgaaaat ggctcggtaa acggaagtgg ctcagtaacc 13440 aacaatattc ttgatcagca acaatggctg gtgggtgaag gcgcagcggc tattgtcgtt 13500 aaaccgtcat cacaagtaac tgccgagcaa gtctatgcgc gtattgatgc agttagtttt 13560 gcccctggta gtgatgctaa agcaattacg attgcggcgg acaaagcatt aacattagcc 13620 ggtatcagtg cggcggatgt agcgagtgtt gaagcacatg caagtggttt tagtgccgaa 13680 aataatgctg aaaaaaccgc gttaccgact ttatacccaa gtgcaaacat cagttcggtg 13740 aaagccaata ttggtcatac gtttaatgca tcgggtatgg cgagtattat taaaactgcc 13800 ttgctgttag atcagaacac gagtcaagat cagaaaagcc aacatattgc tattaacggc 13860 ctaggtcgtg ataacagctg tgcgcatctt atcttatcga gttctgaaca agcacatcaa 13920 gctgcaccag cgcctgtatc tggtatggcc aagcaacgcc cacaattagt taaaaccatc 13980 aaactaggtg gtcaactaat tagcaatgcg attgttaaca gtgcgagttc atctttggat 14040 gcgattaaag cgcagtttgc tggtaagcat ttaaacaaag ttaaccagcc agtaatgatg 14100 gataacctga aaccacaagg cattcgcgct cattcaatga atgaacatgt ggtgacgaga 14160 ccttctagta ctcaagtgtc tcatacccaa gcgttcactc aagcgtctat tcaagcatct 14220 attcaagaac aagag ctagc acaagagcaa gtacaaaata tgcaagctac agcagccgct 14280 gtaagttcac ccctttctca acatcaacat acaacgcagc ccgtagcggc accgagcgtt 14340 gttggagtga ctgtgaaaca taaagcaagt aaccaaattc atcagcaagc gtctacgcat 14400 aaagcatttt tagaaagtcg tttagctgca cagaaaaacc tatcgcaact tgttgaactg 14460 caaaccaagc tatcaacaca aactgctagt gacaatacgt ctaacaatac atctagcaat 14520 acagtgttaa caaatcctgt atcagcaact gcaacgccat taacactcgt atctactctt 14580 gttacaaata agcccgtagt cgtgacaagc ctaaccagca ctgaagcaaa agcgcaagca 14640 gcagctacac aagctggttt tcagataaaa ggacctgttg gttacaacta tccaccactg 14700 cagttaattg agcgttataa caaacctgaa aaagtgattt acgatcaagc tgatttggtt 14760 gaattcgctg aaggtgatat tggtaaggta tttggtgctg aatacaatat tattgatggc 14820 tactcgcgtc gtgtacgtct accaacctca gattatctat tagtaacacg tgttactgaa 14880 cttgatgcca aggtgcatga atacaagaaa tcatacatgt gcactgaata tgatgtacct 14940 gttgatgcac cgttcttaat cgatggtcag atcccttggt ctgttgcagt agaatcaggc 15000 cagtgtgatt taatgttgat ttcatacatc ggtattgatt tccaagctaa aggcgagcgt 15060 gtttaccg tt tacttgattg tgaattaacg ttccttgaag agatggcttt cggtggcgat 15120 actttacgtt acgagatcca cattgattcg tatgcacgta acggcgagca attattattc 15180 ttcttccatt acgattgtta cgtaggggat aagaaggtac ttatcatgcg taatggttgt 15240 gctggtttct ttactgacga agaactttct gacggtaaag gcgttattca taacgacaaa 15300 gacaaagctg aatttagcaa tgctgttaaa tcatcattca cgccgttatt acaacataac 15360 cgtggtcaat acgattataa cgacatgatg aagctggtta atggcgacgt ggcgagttgt 15420 tttggtccgc aatatgatca aggtggccgt aatccatcgt tgaaattctc gtctgagaag 15480 ttcttgatga ttgaacgtat taccaagata gatccaaccg gtggtcattg gggacttggc 15540 ctgttagaag gtcagaaaga tttagaccct gagcattggt atttcccttg tcactttaaa 15600 ggtgatcaag taatggctgg ttcgttgatg tcggaaggtt gtggccaaat ggcgatgttc 15660 ttcatgctgt ctcttggtat gcatactaat gtgaacaacg ctcgtttcca accgctacca 15720 ggtgaatcac aaacggtacg ttgtcgtggg caagtactgc cacagcgcaa taccttaact 15780 taccgtatgg aagtaacggc gatgggtatg catccacagc cattcatgaa agctaatatt 15840 gatattttgc ttgatggtaa agtggttgtt gatttcaaaa acttgagcgt gatgattagc 15900 gaacaagatg aacattcaga ttaccctgta acactgccga gtaatgtgca gcttaaaaca 15960 atttcgtctg ctgctgtaac gcctgttgct gtaactacga cgcctgttgc tttagctaca 16020 acgaacaatg ccgatctgga tgaacgtggt gttgaaccgt ttaagtatcc tgaacgtccg 16080 ttaatgcgcg tagagtcaga cttgtctgca ccgaaaagca aaggtgtgac accgattaag 16140 cattttgaag cgcctgttgt tgctggccac catagagtgc ctaaccaagc accgtttaca 16200 ccttggcata tgtttgagtt tgcgacgggt aatatctcta actgttttgg tcctgacttt 16260 gatgtttatg aaggtcgtat tccaccacgt acgccttgtg gcgatttaca agttgttact 16320 caggttgtgg aagtgcaggg cgaacgtctt gatcttaaaa atccatcaag ctgtatagct 16380 gaatactatg ttcctgaaga tgcttggtac tttactaaaa acagccatga aaactggatg 16440 ccatattcat taatcatgga aattgcattg caaccaaatg gctttatttc tggttacatg 16500 ggcacgacgc ttaaatatcc tgaaaaagat ctgttcttcc gtaaccttga tggtagcggc 16560 acgttattaa agcagattga tttacgcggc aaaacgattg tgaataaatc agtattagtt 16620 agtacggcta ttgctggtgg cgcgattatt caaagcttca cgtttgatat gtctgtagat 16680 ggcgagctat tttatactgg taaagcggta tttggttact ttagtggtga atcgctgac t 16740 aaccaactgg gcattgataa cggtaagacg actaatgctt ggtttgttga taacaatacg 16800 cctgcagcta atattgatgt gttcgattta acggatcagt ctttagcgtt atacaaagcg 16860 cctgtaggta agccgcatta taaattggct ggtggccaga tgaacttcat cgatactgtg 16920 tctgttgttg aaggcggcgg taaagcggat gtggcttatg tttatggcga acgtacgatt 16980 gatgctgatg attggttctt ccgttatcac ttccaccaag atccggtgat gcctggttcg 17040 ttaggtgttg aagctattat tgagctgatg caaacctatg cgcttaaaaa tgacttgggt 17100 gcacagttta ctagtcctcg ttttgttgcg ccgatgacga aagttgattg gaaatatcgt 17160 ggtcaaatta cgccgttgaa taagcagatg tctctggacg tgcatattac cgagatcatt 17220 actgaatcag gtgaagtaag actcgttggt gatgcgaacc tgtctaaaga tggtctgcgt 17280 atttatgaag tgaagaatat tgttttaagt attgttgaag cgtaaaggct cgtgactaaa 17340 cgccgaattg gttaaagacg ccttgcacgc cgtgaatcca tccatggagg cttgaggctg 17400 gcatccatgc cagcaacagc aagcttactt taatcaatac ggcttagtag attatgcgat 17460 cgcattaggt cataaatgaa attgttcggg tgctgaactg aaatatttgg ttaacaacat 17520 aaaagttaaa aacaaatgtt tagttaatag ttcagtagtt acaaaaaagg a attaagaat 17580 gtcgagttta ggttttaaca atagcaatgc catcaattgg gcttggaaag tagatccagc 17640 gtcagttcat acacaggatg ttggcattaa agcagcttta atggatctga ctaatccgct 17700 ttacgtggct aataacgcag gtgttacagg tataacgaat catacgtcag tcgcgggtgc 17760 tattagcaat aatgttgacg ttgatgtact ggcgtttgca caaaagctaa cacctgaaga 17820 tctaggtgat gatgcgtaca agaaacaaca tggggttaaa tatgcatatc atggcggcgc 17880 aatggctaac ggtatcgcgt cggttgaact tgttgtcgca ctaggtaaag cagggttgtt 17940 atgttcgttc ggcgcggcag gtttagtacc tgacgcggtt gaagatgcta ttcgtcgtat 18000 tcaagctgaa ttaccaaatg gcccttacgc ggttaacttg atccatgcac cagcagaaga 18060 agcattagag cgtggggctg ttgaacgttt ccttaaactg ggtgttaaga cggtagaggc 18120 ttcagcttat cttggtttaa ctgagcacat tgtttggtat cgtgttgctg gtttatctaa 18180 aaacagtgat ggctcggtta atattggtaa caaggttatc gctaaagtat cgcgtactga 18240 agttggtcgc cgctttatgg aaccagcacc gcaaaagtta ctggataagt tattagcaca 18300 aaacaagatc acccctgaac aagctgctct agcgctgctt gtacctatgg ctgatgacat 18360 tactgctgaa gctgattctg gtggccatac tgacaaccgt ccat ttttaa ctttattacc 18420 gactatcatc ggactgcgtg atgaagtgca agcgaagtat aacttctctc ctgcattacg 18480 tgttggtgct ggtggcggta tcggaacgcc tgaagcagca ctcgctgcat ttaacatggg 18540 cgctgcttat atcgttctgg gttctgtgaa tcaggcgtgt gttgaagcgg gtgcatctga 18600 atatacgcgt aaactgttat cgacagttga aatggccgat gtgactatgg cacctgctgc 18660 tgatatgttt gaaatgggcg taaagctaca agtattaaaa cgtggttcta tgttcgctat 18720 gcgtgcgaag aaactgtatg acttgtatgt gacttatgac tcgattgaag atatcccagc 18780 tgcggaacgt gagaagattg aaaaacaaat cttccgtgca aacctagacg agatctggga 18840 tggcactatc gctttcttca ctgaacgtga tccagaaatg ctagcccgtg caatgagcag 18900 ccctaaacgt aaaatggcgc ttatcttccg ttggtatctt ggtctttctt cacgctggtc 18960 aaacaccggt gagaagggac gtgaaatgga ttatcaaatt tgggctggcc caagtctagg 19020 cgcatttaac agctgggtga aaggttctta ccttgaagac tatacccgcc gcggcgcagt 19080 tgatgttgct ttgcatatgc ttaaaggtgc tgcgtactta caacgtgtga accagttgaa 19140 gctgcaaggc gttagcttaa gtacagaatt agcaagttat cgtactagtg attaattttt 19200 attaatacat ttgatatatt gaagttataa cggaggg cta gtctttccgt tataacttga 19260 ttcactcttg gatatagtgg ataaatatgt tgcagtactt tacgctaatc gtaaattcat 19320 ttttgtgggc gttatgtgct gaaatggaat atactttaga ttgaaaagtg atagtttgta 19380 atgtagcatc aacagtaata gttagtttct ctcaggatcg cgagtcatga aaaatcaaaa 19440 gataaaagta actcaaaaaa tgatatatag agcagttgct agttctacag caattgaaac 19500 aagagcttct gtaaattcga ttgaaaaaaa gcttaaagct aacaagcata aatacagttc 19560 actatcacta gcctgctagt atatctctaa ctaatctctc catatgctgg tagtcgtgtg 19620 atacaccagc ttgaatggac gctatataaa aatctttatt tttatcccaa agactatagt 19680 ccaataagcc taaccctgct ttgttgcaga tacagtccat aagtaatcga ctcatacgac 19740 catttccttc acgaaaaggg tgtattaaga taaattcaat atggctgcga gctaagtatg 19800 ctactaactc ttcatgattc atatacggca aatttttaaa ttgggataag tattcgcttt 19860 caaatagatc tatacatcga ccaatttgaa gtggtgaagc aaactgaaat ccacctttcc 19920 ccatatcaac gttcctaatt tttccagccc attcatagac gttgccaagc cattgcctat 19980 gccaggttga aatgttttca aaagaaaaat tatcagactc cggaatgctg gtggtaaaac 20040 actgttcgta tagttttgtt aacagtattg cttcaacatc atccatttca gttaggtcaa 20100 taatatcaag cttgtttacc atgactgtat tgttagaccc tagctgatat tgagtttcag 20160 aagcgtcgat ttcgtattta gtcataactg ggtgacatat ttgggaaccg taatatgagt 20220 ttaaagcaca taccaaacaa ttcaacttaa ttttgttcag catgcgcgtg aagaaacttt 20280 ttgacttgga cttaagttaa gacattccag ctgcggagcg tgatccagaa atgctagccc 20340 gtgcaatgag tagccctgaa cgtaaaattg cacttatctt ccgttgtatc ttggtctttc 20400 ttcacgctgg tcaagcatag gcgagaaggg acgtgaaatg gattgtcaga tttgggctgg 20460 cccaagttta ggcgcattca acagctgggt taaaggttct taccttgaag actatacccg 20520 ccgcggcgct gtagatgttg ctttgcatat gcttaaaggt gcagcgtatt tacaacgtgt 20580 aaaccagttg aaactgcaag gcgttagctt aagtacagaa ttggcaagtt atcgtactag 20640 ttttatatgc aatttattga agtagcaata gagtttaaat aaggtttttt aatctttaaa 20700 gtacacgtga tataaacatt catatagtat aaagtgttag attaaataac cactaagaat 20760 atgttttaat tacatttttt aacgttactg atgataaata agttttaaac atacctcttt 20820 taattcataa ttataatctg cacttagtca tactacttta tttaccaatt ggattattta 20880 tggatttcat cgaacgttta ca aggcttat caaaaaaact ttcacaagtt tcaggaacac 20940 tagaaacaga agaagctaca aagaatgctc ttattatgcc atttttgcac actgtactag 21000 gttatgatgt gtttgatcct agtgaagttg tacctgagtt tactgcggat actggtacaa 21060 aaaaaggtga aaaagtagat tatgcattaa tgaaggatgg agaagttcag atccttattg 21120 agtgtaaaaa attaagtgaa aaactatcaa caaaacatgc tagtcaatta ttccgatatt 21180 tctctgttac gaatgcaaga gttgctattc taacaaatgg tcgtgtttat gagttttata 21240 cagatttaga tgcaccgaat aagatggatg aaaaaccatt tttaacatta gaccttgctg 21300 aacttgatga acacatcgtt cctgaaatta agaaattaac taaatcttca ttcgatgttg 21360 attctattgt agatgctgct ggagagctta agtatcttag ccaaattaaa aaggtcttat 21420 ctgaacaatt taagcagcct gaagaagaat ttgttaagtt ttttacttca cgtgtttatg 21480 agggtgttca aacatctaag gttaaagcac aatttctaga tatcacttca aaggctctta 21540 aacaattttt gagcgatagt atcaatgata gattgaagtt tgcacttggc gatagtaaca 21600 atgaacctgt taatgttgta tctaatattt ctgagattaa agttgaagaa atcgttgatg 21660 attctgaaaa agaagtatca aaaattgtaa caactgagtg tgagcttgac ggttttaata 21720 ttgtaaaagc tatat taaga gcaaaggttg atgtctctcg tataacttac agggatacac 21780 aaagctactt tggtgtgtta cttgatgata ataaccgtaa gccattatgt agattacact 21840 ttaatgccaa gcaaaaatat cttggtgttg ttggtgaaga taagaaagag actcgctacc 21900 caatagaaag tttggatgaa ttgtataatc atgcagactt attattaaat accatatcac 21960 aatacgaata attataggaa ctaaaaaggg aattaaaaat tccctttttc atatcatgaa 22020 tatattcttc aatcgaaata ccacaacttt aataatcatt ttctctgttg taattgcatt 22080 tttaagtagc tttaatcaat caatgttcat tcatatttta aactggatgg taatttctta 22140 ttttacattt gaattagttt ataaaataaa aatattgtca tggaaaagct atatatcgtc 22200 gttagggcat aaatttgatt tttttatatt aattatcagc attgccatgc tcgctgctcc 22260 tactgcagaa ttaggtagct tagtatatct ccgagtattt agagttttat cgttatttcg 22320 ggttattaag ttaatcccta atgccgagca cattgttaga ggtgtatcta gagctgtaaa 22380 agcatctaag acagtaatga tattacttgg tgttatgctt gtgttttttt cgatgcttgg 22440 atatacctta ttttcagcat acttacctga gttttttggc gatccgctaa aatcaatgaa 22500 taccgtattt actattttca ctgttgaaaa ttggggtgct gttcctgacg cggctaaaaa 22560 aattggga ct gactatatgt attacgcagt taattcattt gttatcactg tattaatatt 22620 aggtgggttt atagcgttat cattagctaa tgcagttttc gttgatgaaa tggcaagcga 22680 taataatgat gatttaaagc ttgaactaga aaaaataaga caagagaata tagaagtaaa 22740 atcaattctt atggaaatga ataaaaagat taataaattg agtgacgttg ataaactggt 22800 aatgcataaa ttaagagagc aacactcttt ccctgaaata ggtaataagc caacacaaaa 22860 aactaatgtc agttctaaat aagtcaataa accaaacagg ctagcagcat ttagaggtgt 22920 ataaaaacaa tcgcatgggt agtgtacctg tgagtcccta cctatatctt atctcatccc 22980 tattgataaa aagggtgatg tagccaattg ttatgaccct gagcggctca aaaagattga 23040 atccgcagtt gctaaaggtg actataatgc accattgcgt catgcctgtt aatgcatgac 23100 gcaatggtgc ttcgcatcta ttaacaattt ctctaaagtg tacacatctg atggcattaa 23160 tggcgcggga ttaccagcgg ataactttat agtgactaat cttactatcc catccatatt 23220 ccccattgat aagctatcat caactgatag cggtgtttat ttatatcaac catcgtttcg 23280 tttctttaaa gataaaaacc tatctaatca attgaaaagc ggcaagttag atgactttaa 23340 agaagaaggg gtgtttagga ttgctccatt aagtaatgat gaagtgcttt gtatatgagg 23400 actctgctag cagtcaatgt aatagttctt cataacggtt aaaccaagta cagcatcaac 23460 ggtagcgatt gataccttaa taagttttag cgcacaaaat agaactattt aaagatctca 23520 acaaggcgat gatttgataa tgaattattg ccttgttact atcaagagac tttaactgac 23580 tgagatagaa agatatgaca cggaagaaca tattgatcac aggcgcaagt tcagggttgg 23640 gccgaggtat ggccatcgaa tttgcaaaat caggtcataa cttagcactg tgtgcacgta 23700 gacttgataa tttaattgca ctgaaagcag aactcttagc cctcaatcct cacatccaaa 23760 tcgaaatcaa acctcttgat gtcaacgaac atgaacaagt cttcactgtt ttccatgaat 23820 t 23821

【００４４】 <210> 2 <211> 7875 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 atggctaaaa agaacaccac atcgattaag cacgccaagg atgtgttaag tagtgatgat 60 caacagttaa attctcgctt gcaagaatgt ccgattgcca tcattggtat ggcatcggtt 120 tttgcagatg ctaaaaactt ggatcaattc tgggataaca tcgttgactc tgtggacgct 180 attattgatg tgcctagcga tcgctggaac attgacgacc attactcggc agataaaaaa 240 gcagctgaca agacatactg caaacgcggc ggtttcattc cagagcttga ttttgatccg 300 atggaatttg gtttaccgcc aaatatcctc gagttaactg acatcgctca actgttgtca 360 ttaattgttg ctcgtgatgt attaagtgat gctggcattg gtagtgatta tgaccatgat 420 aaaattggta tcacgctggg tgtcggtggt ggtcagaaac aaatttcgcc attaacgtcg 480 cgcctacaag gtccggtatt agaaaaagta ttaaaagctt caggcattga tgaagatgat 540 cgcgctatga tcatcgacaa atttaaaaag gcctacatcg gctgggaaga gaactcattc 600 ccgggcatgc taggtaacgt cattgctggc cgtatcgcca atcgttttga ttttggtggt 660 actaactgtg tggttgatgc ggcatgtgcc ggctcccttg cagcagtaaa aatggcgatt 720 tcggacttac ttgaatatcg ctctgaagta atgatctcgg gtggcgtatg ttgtgataac 780 tcgccattca tgtatatgtc attctcaaaa acaccggcat ttaccaccaa cgatgatatc 840 cgtccgtttg atgacgattc aaaaggcatg ctcgttggtg aaggtatcgg catgatggcg 900 tttaaacgcc ttgaagatgc tgaacgtgac ggcgacaaaa tttattcggt actgaaaggt 960 atcggtacat cttcagatgg tcgtttcaaa tctatttatg ctccgcgccc agatggccaa 1020 gcaaaggcgc tgaaacgtgc ttatgaagat gcaggttttg cccctgaaac ttgtggacta 1080 attgaaggcc atggtacagg tactaaagcc ggtgatgcag cagaatttgc tggtctaact 1140 aaacactttg gcgctgccag tgatgaaaaa caacatatcg cattaggctc agttaaatcg 1200 caaattggtc atacaaaatc tgcggctggc tctgcgggta tgattaaggc ggcattagcc 1260 ctgcatcata aaatcctacc agcaacaatt catatcgata agccaagcga agccttggat 1320 atcaaaaaca gcccgttata cctaaacagc gaaacgcgtc cttggatgcc acgtgaagat 1380 ggtattccac gtcgtgctgg tatcagctca tttggttttg gtggtacgaa cttccatatt 1440 attttagaag agtaccgtcc aggtcacgat agtgcatatc gcttaaattc agtgagtcaa 1500 actgtgttga tctcggcaaa cgatcaacaa ggtatcattg cagaattaaa taactggcgt 1560 actaaactgg ctgtcgatgc cgaacagcaa gcatttgtgt ttaacgactt agtgacaacg 1620 tggccattaa aaacaccatc agttaatcaa gctcgtttag gttttgttgc gcgtaatgcc 1680 aacgaagcga ttgcgatgat tgatacggca ttaaaacaat ttaacgctaa tgcagataaa 1740 accacatggt cagtgcctac cggggtttac tatcgccaag ctggtattga tgcaacaggt 1800 aaagtcgtgg cgctattctc aggacaaggt tcgcaatatg tgaacatggg tcgagaatta 1860 acctgcaact tcccaagcat gatggacagt gctgcggcaa tggataaaga attcagcggg 1920 gctggcttag gtcagttatc tgccgttact ttcccaatcc ctgtttacac ggataccgag 1980 cgtaagctac aagaagatca attacgttta actcaacatg cacaaccagc gattggtagt 2040 ttgagtgttg gtctgttcaa gactttccaa caagcaggct ttaaagctga tttcgctgcc 2100 ggtcatagtt tcggtgaatt aacggcatta tgggctgctg atgtactgag cgaaagcgat 2160 tacatgatgt tagcgcgtag tcgtggtcaa gcaatggctg cacctgagca acaagatttt 2220 gatgcgggta agatggccgc tgttgttggc gatccaaagc aagtagccgt catcattgat 2280 actattgatg atgtctctat tgctaactac aactcgaata accaagttgt tattgcgggt 2340 actactgcgc aggttgctgt agcagttaca accttaggca atgctggttt caaagttgtg 2400 ccactgccgg tatccgctgc gttccataca cctttagtgg gtcatgctca aaaaccattt 2460 gctaaagcgg ttgatcgcgc taaatttaaa gcgccaagca tccctgtatt tgctaatggc 2520 acaggcttag ttcattcaag taaaccgaat gacattaaga aaaacctgaa aaaccacatg 2580 ttagaatctg ttcatttcaa tcaagaaatt gacaacatct atgctgatgg tggtcgcgta 2640 tttatcgaat ttggtccgaa gaatgtatta actaaattgg ttgaaaacat tctcactgac 2700 aaatctgatg tgactgctat cgcggttaat gctaatccaa aacaacctgc tgatgtacaa 2760 atgcgtcaag ctgcgctgca aatggcagtg cttggtgtcg cattagacaa tattgacccg 2820 tacgacgcag ttaagcgccc acttgttgcg ccaaaagcat cgccaatgtt gatgaaatta 2880 tctgcagcgt cttatgttag tccaaaaacg aagaaagcgt ttgcagatgc attaacggat 2940 ggttggacgg tgaaacaagc caaagccgca ccagttgttg tgtcacaacc acaagtgatt 3000 gaaaagatcg ttgaagttga aaagatagtc gaacgcattg tcgaagttga gcgtatcgtt 3060 gaagtagaaa aaattgtcta cgttaatgct gatggttcac atgcaacgac gaacaatagc 3120 gctattaata atggcatgca tagctctgac gccgatctcg ttgcctcaat tgaacagagt 3180 gttggtcagt ttgttgcaca ccaacagcaa ttattaaatg tacatgaaca gtttatgcaa 3240 ggtccacaag actatgcaaa aacagtgcaa aacgtacttg ctgcgcaaac caataatgaa 3300 ttaccggaaa gtttagaccg tacattgtct atgtataacg agttccaatc agaaacgcta 3360 cgtgtacatg aaacgtacct gaacaatcag acgagcagca tgaacactat gcttactggt 3420 gctgaaactg atgtgctagt agctcctgtc actcaagtat tgaatacacc cgttgccgct 3480 agtcacaagg cagttgctcc agttattgct aatacagtga cgaatgttgt atctagtgtc 3540 agtaataacg cggcggttgc agtgcaaact gtggcattag cgcctacgca agaaatcgct 3600 ccagcagtcg ttactacgcc agcacccgca ttggttgcta ttgtggctga acctgtgatt 3660 gttgcgcctg ttgctacaga agttgcacca attacatcat cagttacacc tgttgtcgca 3720 actcaagcgg ctatcgatgt agcaactatt aacaaagtaa tgttagaagt tgttgctgat 3780 aaaaccggtt atccaacaga catgcttgaa ctgagcatgg acatggaagc tgatttaggt 3840 atcgactcaa tcaaacgtgt tgaaattcta ggcgcagtac aggaattgat ccctgactta 3900 cctgagctta accctgaaga tcttgctgag ctacgtacgc ttggtgagat tgtcgattat 3960 atgaatgtta aagcgcaaga ggctgctcct gtatcagcac ctgcatctgc tggtattgat 4020 ttagcccaca tccaaaacgt aatgttagaa gtggttgcag acaaaaccgg ttacccaact 4080 gacatgcttg aactgagcat ggacatggaa gctgacttag gtattgattc aatcaaacgt 4140 gtggaaatct taggcgcagt gcaggagatc atcactgatt tacctgagct aaatcctgaa 4200 gacttagctg agctacgcac cttaggtgaa atcgttagtt acatgcaaag caaagcgccc 4260 gtcgctgaaa gtgtgactgt tacagattca gacgttactt ctgtcgcaag ctctgcacca 4320 tctatcgatt tgaaccacat tcaaacagtg atgatggatg tagttgcaga taagactggt 4380 tatccaacag atatgttaga acttggcatg gacatggaag ctgacttagg tatcgattca 4440 atcaaacgtg tggaaatatt aggcgcagtg caggagatca ttactgattt acctgagcta 4500 aaccctgaag atcttgctga attacgcacg ctaggtgaaa tcgttagcta catgcaaagc 4560 aaagcgcctg tagctgaagc gcctgcagct gtagaaagtg cacctattgg tgtaacaagt 4620 tcagcaccgt ctatcgattt aaaccacatc caaactgtga tgatggaagt tgttgcagac 4680 aaaaccggtt atccagtaga catgttagaa cttgctatgg acatggaagc tgaccttggt 4740 atcgactcaa tcaaacgtgt agaaattcta ggtgcggtac aggaaatcat cactgactta 4800 cctgagctaa accctgaaga tcttgctgaa ctacgtacgt taggtgaaat cgttagttac 4860 atgcaaagca aagtgcccgt agctgaagcg cctgttgctg tagaaagtgc acatactggt 4920 gtaacaagtt cagcaccgtc tatcgattta gaccatatcc aaaacgtaat gatggatgtt 4980 gttgctgata agactggtta tcctgccaat atgcttgaac tagcaatgga catggaagct 5040 gaccttggta ttgattcaat caagcgtgtt gaaattctag gcgcggtaca ggagatcatt 5100 actgatttac ctgaactaaa cccagaagac ttagcggaac tacgtacgtt agaagaaatt 5160 gtaacttaca tgcagagtaa agctcgtggt gttactgcaa atgtagtagc taatcatgaa 5220 cctgaaaaaa gcgcagtgtc agatgcattt atgcagaaca gcgttgctac tatcacggca 5280 gctgtagcgc caaaagcaga gtttcaacca gcgccgagcg caacggttgc tatctcgcgt 5340 ctaagctcta tcagtaaaat aagccaaaat tgccaaggcg ctaatgcctt aatcgtggct 5400 gatggcactg ataatgcagt attactcgca gaccacctac tgcaaactgg ctggaatgta 5460 actgcattgc aaccaacttg ggtagcagta acaacgacga aagcatttaa tcaatcagta 5520 aacctggtga gcttaaatgg cgttgatgaa actgaaatca acaacatcat tgctgctaac 5580 gcacaattgg atgcagttat ctatctgcac gccagtagcg aaattaatgc tattgaatac 5640 ccacaagcat ctaagcaagg cctgatgtta gccttcttat tagcgaaatt gagtaaagta 5700 actcaagccg ctaaagtgcg tggttctttc atggtagtta ctcagcaggg cggttcactt 5760 ggttttagca atgacgcaca agctgaccta gtacaggcag acttagtaca gacagacctt 5820 gtacaaagcg gcttaaacgg tttagttaaa acactgtcac acgaatggga taacgtattc 5880 tgtcgtgcgg tagatatgcc aacatcatta gctgcagcgc aagtagcaac tcttgtttgt 5940 gatgaactgt ttgacgcaga tagcgtgtta actgaagtag gttatcaaca agcagagcaa 6000 aatggtgttg cccgcattac cttaactggt gttgcgactg acagttatgc actgactgct 6060 ggtaataaca ttgatgctaa ttcggtattc ttagtgagtg gtggcgcaaa aggcgtaaca 6120 gcccattgcg tagtacgtat cgctgaagag tatcaagcta agttcatctt attaggacgc 6180 tcaaaatttt caaatgatga gccgagctgg gcaaacggta ttactgaaga agcggcatta 6240 aagaaagcag caatgcaggc tttaattgca gcaggtgata aaccaacgcc cgttaagatc 6300 gtacagttaa tcaaaccaat tcaagctaat cgtgaaattg cgcaaacttt gtctgcaatt 6360 accgctgctg gtggccaagc tgaatatgtt tctgcagatg taaccaatgc agcaagcgta 6420 caaacggcag tcactccagc tatcgctaag ttcggtgcta tcactggcat cattcatggc 6480 gctggcgttt tagctgacca attcattgag caaaaaacac tgagcgattt tgagtctgta 6540 tacagtacta aaatcgatgg tttgttatca ctgttatcag taactgaagc aagcaacatc 6600 aagcaattgg tattgttctc gtcagcggct ggtttctacg gtaaccctgg ccaatctgat 6660 tactcgattg ccaatgaaat cttaaacaag acggcatacc gctttaaatc attgcaccca 6720 caagctcaag tcttgagttt taactggggt ccttgggacg gtggcatggt aacgcctgag 6780 ctaaaacgta tgtttgatca gcgtggtgtt tacattattc cacttgatgc aggtgcgcag 6840 ttgttactta atgaattagc cgctaatgat aatcgttgtc cacaaatcct agtgggtaat 6900 gacttatcta aagatgctgc tgatgaaaag agtactgctg taaaaaagcc acaagttagt 6960 cgtttatcag atgctttagt aactaaaagc atcaaagcga ctaacagtga ctctttgaac 7020 gatcattttc aagtgaacga caaccgcttt ttagctgacc acatgatcaa aggcaatcag 7080 gtattaccaa cggtatgcgc gattgcttgg atgagtgatg cagcacaagc aacttatagt 7140 aaccgagact gtgcattgaa atatgtcggt ttcgaagact acaaattgtt taaaggggta 7200 gtttttgatg gtaatgaggt ggcagattat caaatccaat tgtcgcctgt ggccgctgtt 7260 tcagagcatg aaacagtagt ccgtattgcc gcaaaagtgt ttagcctgaa aagcgatggt 7320 aagccagtat ttcattatgc cgcaacaata ttgttagctt ctcagcctct tgatgcggta 7380 acgctgcaat taccggtact agctgtagat attaacaatt tggttagcga tgaagcacaa 7440 gcgttataca gcaatggcac cttgttccac ggtgaaagtc tgcagggtat taagcagata 7500 ttaagttgtg acgacaatgg cctgctattg gcttgtcaga taacagatgt tgcagcagct 7560 aagcagggtg cattccccgt agctgccaac aatatctttg ctaatgattt ggtttatcag 7620 gcgatgttgg tctgggtgcg caagcaattt ggtttaggta gcttaccttc ggtaacagct 7680 gcttggactg tgtatcgtga agtggctgtc gatgaagtgt tttatctgca actcaatgtt 7740 gttgaacacg atctattggg ttctcgcggc agtaaagccc gttgtgatat tcaattgatt 7800 gctgcagata tgcagatact tgcagaagtt aaatcagcgc aagttagtgt cagtgacatt 7860 ttgaacgata tgtct 7875<210> 2 <211> 7875 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> ggatcaattc tgggataaca tcgttgactc tgtggacgct 180 attattgatg tgcctagcga tcgctggaac attgacgacc attactcggc agataaaaaa 240 gcagctgaca agacatactg caaacgcggc ggtttcattc cagagcttga ttttgatccg 300 atggaatttg gtttaccgcc aaatatcctc gagttaactg acatcgctca actgttgtca 360 ttaattgttg ctcgtgatgt attaagtgat gctggcattg gtagtgatta tgaccatgat 420 aaaattggta tcacgctggg tgtcggtggt ggtcagaaac aaatttcgcc attaacgtcg 480 cgcctacaag gtccggtatt agaaaaagta ttaaaagctt caggcattga tgaagatgat 540 cgcgctatga tcatcgacaa atttaaaaag gcctacatcg gctgggaaga gaactcattc 600 ccgggcatgc taggtaacgt cattgctggc cgtatcgcca atcgttttga ttttggtggt 660 actaactgtg tggttgatgc ggcatgtgcc ggctcccttg cagcagtaaa aatggcgat ctg gg ctg ctg ctg ctg ctg ctg ctg gc gg gataac 780 tcgccattca tgtatatgtc attctcaaaa acaccggcat ttaccaccaa cgatgatatc 840 cgtccgtttg atgacgattc aaaaggcatg ctcgttggtg aaggtatcgg catgatggcg 900 tttaaacgcc ttgaagatgc tgaacgtgac ggcgacaaaa tttattcggt actgaaaggt 960 atcggtacat cttcagatgg tcgtttcaaa tctatttatg ctccgcgccc agatggccaa 1020 gcaaaggcgc tgaaacgtgc ttatgaagat gcaggttttg cccctgaaac ttgtggacta 1080 attgaaggcc atggtacagg tactaaagcc ggtgatgcag cagaatttgc tggtctaact 1140 aaacactttg gcgctgccag tgatgaaaaa caacatatcg cattaggctc agttaaatcg 1200 caaattggtc atacaaaatc tgcggctggc tctgcgggta tgattaaggc ggcattagcc 1260 ctgcatcata aaatcctacc agcaacaatt catatcgata agccaagcga agccttggat 1320 atcaaaaaca gcccgttata cctaaacagc gaaacgcgtc cttggatgcc acgtgaagat 1380 ggtattccac gtcgtgctgg tatcagctca tttggttttg gtggtacgaa cttccatatt 1440 attttagaag agtaccgtcc aggtcacgat agtgcatatc gcttaaattc agtgagtcaa 1500 actgtgttga tctcggcaaa cgatcaacaa ggtatcattg cagaattaaa taactggcgt 1560 actaaactgg ctgtcgatgc cgaacagcaa gcatttgtgt ttaacgactt agtgacaacg 162 0 tggccattaa aaacaccatc agttaatcaa gctcgtttag gttttgttgc gcgtaatgcc 1680 aacgaagcga ttgcgatgat tgatacggca ttaaaacaat ttaacgctaa tgcagataaa 1740 accacatggt cagtgcctac cggggtttac tatcgccaag ctggtattga tgcaacaggt 1800 aaagtcgtgg cgctattctc aggacaaggt tcgcaatatg tgaacatggg tcgagaatta 1860 acctgcaact tcccaagcat gatggacagt gctgcggcaa tggataaaga attcagcggg 1920 gctggcttag gtcagttatc tgccgttact ttcccaatcc ctgtttacac ggataccgag 1980 cgtaagctac aagaagatca attacgttta actcaacatg cacaaccagc gattggtagt 2040 ttgagtgttg gtctgttcaa gactttccaa caagcaggct ttaaagctga tttcgctgcc 2100 ggtcatagtt tcggtgaatt aacggcatta tgggctgctg atgtactgag cgaaagcgat 2160 tacatgatgt tagcgcgtag tcgtggtcaa gcaatggctg cacctgagca acaagatttt 2220 gatgcgggta agatggccgc tgttgttggc gatccaaagc aagtagccgt catcattgat 2280 actattgatg atgtctctat tgctaactac aactcgaata accaagttgt tattgcgggt 2340 actactgcgc aggttgctgt agcagttaca accttaggca atgctggttt caaagttgtg 2400 ccactgccgg tatccgctgc gttccataca cctttagtgg gtcatgctca aaaaccattt 2460 gcta aagcgg ttgatcgcgc taaatttaaa gcgccaagca tccctgtatt tgctaatggc 2520 acaggcttag ttcattcaag taaaccgaat gacattaaga aaaacctgaa aaaccacatg 2580 ttagaatctg ttcatttcaa tcaagaaatt gacaacatct atgctgatgg tggtcgcgta 2640 tttatcgaat ttggtccgaa gaatgtatta actaaattgg ttgaaaacat tctcactgac 2700 aaatctgatg tgactgctat cgcggttaat gctaatccaa aacaacctgc tgatgtacaa 2760 atgcgtcaag ctgcgctgca aatggcagtg cttggtgtcg cattagacaa tattgacccg 2820 tacgacgcag ttaagcgccc acttgttgcg ccaaaagcat cgccaatgtt gatgaaatta 2880 tctgcagcgt cttatgttag tccaaaaacg aagaaagcgt ttgcagatgc attaacggat 2940 ggttggacgg tgaaacaagc caaagccgca ccagttgttg tgtcacaacc acaagtgatt 3000 gaaaagatcg ttgaagttga aaagatagtc gaacgcattg tcgaagttga gcgtatcgtt 3060 gaagtagaaa aaattgtcta cgttaatgct gatggttcac atgcaacgac gaacaatagc 3120 gctattaata atggcatgca tagctctgac gccgatctcg ttgcctcaat tgaacagagt 3180 gttggtcagt ttgttgcaca ccaacagcaa ttattaaatg tacatgaaca gtttatgcaa 3240 ggtccacaag actatgcaaa aacagtgcaa aacgtacttg ctgcgcaaac caataatgaa 3300 ttaccggaaa gtttagaccg tacattgtct atgtataacg agttccaatc agaaacgcta 3360 cgtgtacatg aaacgtacct gaacaatcag acgagcagca tgaacactat gcttactggt 3420 gctgaaactg atgtgctagt agctcctgtc actcaagtat tgaatacacc cgttgccgct 3480 agtcacaagg cagttgctcc agttattgct aatacagtga cgaatgttgt atctagtgtc 3540 agtaataacg cggcggttgc agtgcaaact gtggcattag cgcctacgca agaaatcgct 3600 ccagcagtcg ttactacgcc agcacccgca ttggttgcta ttgtggctga acctgtgatt 3660 gttgcgcctg ttgctacaga agttgcacca attacatcat cagttacacc tgttgtcgca 3720 actcaagcgg ctatcgatgt agcaactatt aacaaagtaa tgttagaagt tgttgctgat 3780 aaaaccggtt atccaacaga catgcttgaa ctgagcatgg acatggaagc tgatttaggt 3840 atcgactcaa tcaaacgtgt tgaaattcta ggcgcagtac aggaattgat ccctgactta 3900 cctgagctta accctgaaga tcttgctgag ctacgtacgc ttggtgagat tgtcgattat 3960 atgaatgtta aagcgcaaga ggctgctcct gtatcagcac ctgcatctgc tggtattgat 4020 ttagcccaca tccaaaacgt aatgttagaa gtggttgcag acaaaaccgg ttacccaact 4080 gacatgcttg aactgagcat ggacatggaa gctgacttag gtattgattc aatcaaacgt 4140 gtggaaatct taggc gcagt gcaggagatc atcactgatt tacctgagct aaatcctgaa 4200 gacttagctg agctacgcac cttaggtgaa atcgttagtt acatgcaaag caaagcgccc 4260 gtcgctgaaa gtgtgactgt tacagattca gacgttactt ctgtcgcaag ctctgcacca 4320 tctatcgatt tgaaccacat tcaaacagtg atgatggatg tagttgcaga taagactggt 4380 tatccaacag atatgttaga acttggcatg gacatggaag ctgacttagg tatcgattca 4440 atcaaacgtg tggaaatatt aggcgcagtg caggagatca ttactgattt acctgagcta 4500 aaccctgaag atcttgctga attacgcacg ctaggtgaaa tcgttagcta catgcaaagc 4560 aaagcgcctg tagctgaagc gcctgcagct gtagaaagtg cacctattgg tgtaacaagt 4620 tcagcaccgt ctatcgattt aaaccacatc caaactgtga tgatggaagt tgttgcagac 4680 aaaaccggtt atccagtaga catgttagaa cttgctatgg acatggaagc tgaccttggt 4740 atcgactcaa tcaaacgtgt agaaattcta ggtgcggtac aggaaatcat cactgactta 4800 cctgagctaa accctgaaga tcttgctgaa ctacgtacgt taggtgaaat cgttagttac 4860 atgcaaagca aagtgcccgt agctgaagcg cctgttgctg tagaaagtgc acatactggt 4920 gtaacaagtt cagcaccgtc tatcgattta gaccatatcc aaaacgtaat gatggatgtt 4980 gttgctgata agactggtta tcctgccaat atgcttgaac tagcaatgga catggaagct 5040 gaccttggta ttgattcaat caagcgtgtt gaaattctag gcgcggtaca ggagatcatt 5100 actgatttac ctgaactaaa cccagaagac ttagcggaac tacgtacgtt agaagaaatt 5160 gtaacttaca tgcagagtaa agctcgtggt gttactgcaa atgtagtagc taatcatgaa 5220 cctgaaaaaa gcgcagtgtc agatgcattt atgcagaaca gcgttgctac tatcacggca 5280 gctgtagcgc caaaagcaga gtttcaacca gcgccgagcg caacggttgc tatctcgcgt 5340 ctaagctcta tcagtaaaat aagccaaaat tgccaaggcg ctaatgcctt aatcgtggct 5400 gatggcactg ataatgcagt attactcgca gaccacctac tgcaaactgg ctggaatgta 5460 actgcattgc aaccaacttg ggtagcagta acaacgacga aagcatttaa tcaatcagta 5520 aacctggtga gcttaaatgg cgttgatgaa actgaaatca acaacatcat tgctgctaac 5580 gcacaattgg atgcagttat ctatctgcac gccagtagcg aaattaatgc tattgaatac 5640 ccacaagcat ctaagcaagg cctgatgtta gccttcttat tagcgaaatt gagtaaagta 5700 actcaagccg ctaaagtgcg tggttctttc atggtagtta ctcagcaggg cggttcactt 5760 ggttttagca atgacgcaca agctgaccta gtacaggcag acttagtaca gacagacctt 5820 gtacaaagcg gcttaaacgg tttagt taaa acactgtcac acgaatggga taacgtattc 5880 tgtcgtgcgg tagatatgcc aacatcatta gctgcagcgc aagtagcaac tcttgtttgt 5940 gatgaactgt ttgacgcaga tagcgtgtta actgaagtag gttatcaaca agcagagcaa 6000 aatggtgttg cccgcattac cttaactggt gttgcgactg acagttatgc actgactgct 6060 ggtaataaca ttgatgctaa ttcggtattc ttagtgagtg gtggcgcaaa aggcgtaaca 6120 gcccattgcg tagtacgtat cgctgaagag tatcaagcta agttcatctt attaggacgc 6180 tcaaaatttt caaatgatga gccgagctgg gcaaacggta ttactgaaga agcggcatta 6240 aagaaagcag caatgcaggc tttaattgca gcaggtgata aaccaacgcc cgttaagatc 6300 gtacagttaa tcaaaccaat tcaagctaat cgtgaaattg cgcaaacttt gtctgcaatt 6360 accgctgctg gtggccaagc tgaatatgtt tctgcagatg taaccaatgc agcaagcgta 6420 caaacggcag tcactccagc tatcgctaag ttcggtgcta tcactggcat cattcatggc 6480 gctggcgttt tagctgacca attcattgag caaaaaacac tgagcgattt tgagtctgta 6540 tacagtacta aaatcgatgg tttgttatca ctgttatcag taactgaagc aagcaacatc 6600 aagcaattgg tattgttctc gtcagcggct ggtttctacg gtaaccctgg ccaatctgat 6660 tactcgattg ccaatgaaat cttaaacaag a cggcatacc gctttaaatc attgcaccca 6720 caagctcaag tcttgagttt taactggggt ccttgggacg gtggcatggt aacgcctgag 6780 ctaaaacgta tgtttgatca gcgtggtgtt tacattattc cacttgatgc aggtgcgcag 6840 ttgttactta atgaattagc cgctaatgat aatcgttgtc cacaaatcct agtgggtaat 6900 gacttatcta aagatgctgc tgatgaaaag agtactgctg taaaaaagcc acaagttagt 6960 cgtttatcag atgctttagt aactaaaagc atcaaagcga ctaacagtga ctctttgaac 7020 gatcattttc aagtgaacga caaccgcttt ttagctgacc acatgatcaa aggcaatcag 7080 gtattaccaa cggtatgcgc gattgcttgg atgagtgatg cagcacaagc aacttatagt 7140 aaccgagact gtgcattgaa atatgtcggt ttcgaagact acaaattgtt taaaggggta 7200 gtttttgatg gtaatgaggt ggcagattat caaatccaat tgtcgcctgt ggccgctgtt 7260 tcagagcatg aaacagtagt ccgtattgcc gcaaaagtgt ttagcctgaa aagcgatggt 7320 aagccagtat ttcattatgc cgcaacaata ttgttagctt ctcagcctct tgatgcggta 7380 acgctgcaat taccggtact agctgtagat attaacaatt tggttagcga tgaagcacaa 7440 gcgttataca gcaatggcac cttgttccac ggtgaaagtc tgcagggtat taagcagata 7500 ttaagttgtg acgacaatgg cctgctattg gcttgtc aga taacagatgt tgcagcagct 7560 aagcagggtg cattccccgt agctgccaac aatatctttg ctaatgattt ggtttatcag 7620 gcgatgttgg tctgggtgcg caagcaattt ggtttaggta gcttaccttc ggtaacagct 7680 gcttggactg tgtatcgtga agtggctgtc gatgaagtgt tttatctgca actcaatgtt 7740 gttgaacacg atctattggg ttctcgcggc agtaaagccc gttgtgatat tcaattgatt 7800 gctgcagata tgcagatact tgcagaagtt aaatcagcgc aagttagtgt cagtgacatt 7860 ttgaacgata tgtct 7875

【００４５】 <210> 3 <211> 2625 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 Met Ala Lys Lys Asn Thr Thr Ser Ile Lys His Ala Lys Asp Val Leu 1 5 10 15 Ser Ser Asp Asp Gln Gln Leu Asn Ser Arg Leu Gln Glu Cys Pro Ile 20 25 30 Ala Ile Ile Gly Met Ala Ser Val Phe Ala Asp Ala Lys Asn Leu Asp 35 40 45 Gln Phe Trp Asp Asn Ile Val Asp Ser Val Asp Ala Ile Ile Asp Val 50 55 60 Pro Ser Asp Arg Trp Asn Ile Asp Asp His Tyr Ser Ala Asp Lys Lys 65 70 75 80 Ala Ala Asp Lys Thr Tyr Cys Lys Arg Gly Gly Phe Ile Pro Glu Leu 85 90 95 Asp Phe Asp Pro Met Glu Phe Gly Leu Pro Pro Asn Ile Leu Glu Leu 100 105 110 Thr Asp Ile Ala Gln Leu Leu Ser Leu Ile Val Ala Arg Asp Val Leu 115 120 125 Ser Asp Ala Gly Ile Gly Ser Asp Tyr Asp His Asp Lys Ile Gly Ile 130 135 140 Thr Leu Gly Val Gly Gly Gly Gln Lys Gln Ile Ser Pro Leu Thr Ser 145 150 155 160 Arg Leu Gln Gly Pro Val Leu Glu Lys Val Leu Lys Ala Ser Gly Ile 165 170 175 Asp Glu Asp Asp Arg Ala Met Ile Ile Asp Lys Phe Lys Lys Ala Tyr 180 185 190 Ile Gly Trp Glu Glu Asn Ser Phe Pro Gly Met Leu Gly Asn Val Ile 195 200 205 Ala Gly Arg Ile Ala Asn Arg Phe Asp Phe Gly Gly Thr Asn Cys Val 210 215 220 Val Asp Ala Ala Cys Ala Gly Ser Leu Ala Ala Val Lys Met Ala Ile 225 230 235 240 Ser Asp Leu Leu Glu Tyr Arg Ser Glu Val Met Ile Ser Gly Gly Val 245 250 255 Cys Cys Asp Asn Ser Pro Phe Met Tyr Met Ser Phe Ser Lys Thr Pro 260 265 270 Ala Phe Thr Thr Asn Asp Asp Ile Arg Pro Phe Asp Asp Asp Ser Lys 275 280 285 Gly Met Leu Val Gly Glu Gly Ile Gly Met Met Ala Phe Lys Arg Leu 290 295 300 Glu Asp Ala Glu Arg Asp Gly Asp Lys Ile Tyr Ser Val Leu Lys Gly 305 310 315 320 Ile Gly Thr Ser Ser Asp Gly Arg Phe Lys Ser Ile Tyr Ala Pro Arg 325 330 335 Pro Asp Gly Gln Ala Lys Ala Leu Lys Arg Ala Tyr Glu Asp Ala Gly 340 345 350 Phe Ala Pro Glu Thr Cys Gly Leu Ile Glu Gly His Gly Thr Gly Thr 355 360 365 Lys Ala Gly Asp Ala Ala Glu Phe Ala Gly Leu Thr Lys His Phe Gly 370 375 380 Ala Ala Ser Asp Glu Lys Gln His Ile Ala Leu Gly Ser Val Lys Ser 385 390 395 400 Gln Ile Gly His Thr Lys Ser Ala Ala Gly Ser Ala Gly Met Ile Lys 405 410 415 Ala Ala Leu Ala Leu His His Lys Ile Leu Pro Ala Thr Ile His Ile 420 425 430 Asp Lys Pro Ser Glu Ala Leu Asp Ile Lys Asn Ser Pro Leu Tyr Leu 435 440 445 Asn Ser Glu Thr Arg Pro Trp Met Pro Arg Glu Asp Gly Ile Pro Arg 450 455 460 Arg Ala Gly Ile Ser Ser Phe Gly Phe Gly Gly Thr Asn Phe His Ile 465 470 475 480 Ile Leu Glu Glu Tyr Arg Pro Gly His Asp Ser Ala Tyr Arg Leu Asn 485 490 495 Ser Val Ser Gln Thr Val Leu Ile Ser Ala Asn Asp Gln Gln Gly Ile 500 505 510 Ile Ala Glu Leu Asn Asn Trp Arg Thr Lys Leu Ala Val Asp Ala Glu 515 520 525 Gln Gln Ala Phe Val Phe Asn Asp Leu Val Thr Thr Trp Pro Leu Lys 530 535 540 Thr Pro Ser Val Asn Gln Ala Arg Leu Gly Phe Val Ala Arg Asn Ala 545 550 555 560 Asn Glu Ala Ile Ala Met Ile Asp Thr Ala Leu Lys Gln Phe Asn Ala 565 570 575 Asn Ala Asp Lys Thr Thr Trp Ser Val Pro Thr Gly Val Tyr Tyr Arg 580 585 590 Gln Ala Gly Ile Asp Ala Thr Gly Lys Val Val Ala Leu Phe Ser Gly 595 600 605 Gln Gly Ser Gln Tyr Val Asn Met Gly Arg Glu Leu Thr Cys Asn Phe 610 615 620 Pro Ser Met Met Asp Ser Ala Ala Ala Met Asp Lys Glu Phe Ser Gly 625 630 635 640 Ala Gly Leu Gly Gln Leu Ser Ala Val Thr Phe Pro Ile Pro Val Tyr 645 650 655 Thr Asp Thr Glu Arg Lys Leu Gln Glu Asp Gln Leu Arg Leu Thr Gln 660 665 670 His Ala Gln Pro Ala Ile Gly Ser Leu Ser Val Gly Leu Phe Lys Thr 675 680 685 Phe Gln Gln Ala Gly Phe Lys Ala Asp Phe Ala Ala Gly His Ser Phe 690 695 700 Gly Glu Leu Thr Ala Leu Trp Ala Ala Asp Val Leu Ser Glu Ser Asp 705 710 715 720 Tyr Met Met Leu Ala Arg Ser Arg Gly Gln Ala Met Ala Ala Pro Glu 725 730 735 Gln Gln Asp Phe Asp Ala Gly Lys Met Ala Ala Val Val Gly Asp Pro 740 745 750 Lys Gln Val Ala Val Ile Ile Asp Thr Ile Asp Asp Val Ser Ile Ala 755 760 765 Asn Tyr Asn Ser Asn Asn Gln Val Val Ile Ala Gly Thr Thr Ala Gln 770 775 780 Val Ala Val Ala Val Thr Thr Leu Gly Asn Ala Gly Phe Lys Val Val 785 790 795 800 Pro Leu Pro Val Ser Ala Ala Phe His Thr Pro Leu Val Gly His Ala 805 810 815 Gln Lys Pro Phe Ala Lys Ala Val Asp Arg Ala Lys Phe Lys Ala Pro 820 825 830 Ser Ile Pro Val Phe Ala Asn Gly Thr Gly Leu Val His Ser Ser Lys 835 840 845 Pro Asn Asp Ile Lys Lys Asn Leu Lys Asn His Met Leu Glu Ser Val 850 855 860 His Phe Asn Gln Glu Ile Asp Asn Ile Tyr Ala Asp Gly Gly Arg Val 865 870 875 880 Phe Ile Glu Phe Gly Pro Lys Asn Val Leu Thr Lys Leu Val Glu Asn 885 890 895 Ile Leu Thr Asp Lys Ser Asp Val Thr Ala Ile Ala Val Asn Ala Asn 900 905 910 Pro Lys Gln Pro Ala Asp Val Gln Met Arg Gln Ala Ala Leu Gln Met 915 920 925 Ala Val Leu Gly Val Ala Leu Asp Asn Ile Asp Pro Tyr Asp Ala Val 930 935 940 Lys Arg Pro Leu Val Ala Pro Lys Ala Ser Pro Met Leu Met Lys Leu 945 950 955 960 Ser Ala Ala Ser Tyr Val Ser Pro Lys Thr Lys Lys Ala Phe Ala Asp 965 970 975 Ala Leu Thr Asp Gly Trp Thr Val Lys Gln Ala Lys Ala Ala Pro Val 980 985 990 Val Val Ser Gln Pro Gln Val Ile Glu Lys Ile Val Glu Val Glu Lys 995 1000 1005 Ile Val Glu Arg Ile Val Glu Val Glu Arg Ile Val Glu Val Glu Lys 1010 1015 1020 Ile Val Tyr Val Asn Ala Asp Gly Ser His Ala Thr Thr Asn Asn Ser 1025 1030 1035 1040 Ala Ile Asn Asn Gly Met His Ser Ser Asp Ala Asp Leu Val Ala Ser 1045 1050 1055 Ile Glu Gln Ser Val Gly Gln Phe Val Ala His Gln Gln Gln Leu Leu 1060 1065 1070 Asn Val His Glu Gln Phe Met Gln Gly Pro Gln Asp Tyr Ala Lys Thr 1075 1080 1085 Val Gln Asn Val Leu Ala Ala Gln Thr Asn Asn Glu Leu Pro Glu Ser 1090 1095 1100 Leu Asp Arg Thr Leu Ser Met Tyr Asn Glu Phe Gln Ser Glu Thr Leu 1105 1110 1115 1120 Arg Val His Glu Thr Tyr Leu Asn Asn Gln Thr Ser Ser Met Asn Thr 1125 1130 1135 Met Leu Thr Gly Ala Glu Thr Asp Val Leu Val Ala Pro Val Thr Gln 1140 1145 1150 Val Leu Asn Thr Pro Val Ala Ala Ser His Lys Ala Val Ala Pro Val 1155 1160 1165 Ile Ala Asn Thr Val Thr Asn Val Val Ser Ser Val Ser Asn Asn Ala 1170 1175 1180 Ala Val Ala Val Gln Thr Val Ala Leu Ala Pro Thr Gln Glu Ile Ala 1185 1190 1195 1200 Pro Ala Val Val Thr Thr Pro Ala Pro Ala Leu Val Ala Ile Val Ala 1205 1210 1215 Glu Pro Val Ile Val Ala Pro Val Ala Thr Glu Val Ala Pro Ile Thr 1220 1225 1230 Ser Ser Val Thr Pro Val Val Ala Thr Gln Ala Ala Ile Asp Val Ala 1235 1240 1245 Thr Ile Asn Lys Val Met Leu Glu Val Val Ala Asp Lys Thr Gly Tyr 1250 1255 1260 Pro Thr Asp Met Leu Glu Leu Ser Met Asp Met Glu Ala Asp Leu Gly 1265 1270 1275 1280 Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly Ala Val Gln Glu Leu 1285 1290 1295 Ile Pro Asp Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Leu Arg 1300 1305 1310 Thr Leu Gly Glu Ile Val Asp Tyr Met Asn Val Lys Ala Gln Glu Ala 1315 1320 1325 Ala Pro Val Ser Ala Pro Ala Ser Ala Gly Ile Asp Leu Ala His Ile 1330 1335 1340 Gln Asn Val Met Leu Glu Val Val Ala Asp Lys Thr Gly Tyr Pro Thr 1345 1350 1355 1360 Asp Met Leu Glu Leu Ser Met Asp Met Glu Ala Asp Leu Gly Ile Asp 1365 1370 1375 Ser Ile Lys Arg Val Glu Ile Leu Gly Ala Val Gln Glu Ile Ile Thr 1380 1385 1390 Asp Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Leu Arg Thr Leu 1395 1400 1405 Gly Glu Ile Val Ser Tyr Met Gln Ser Lys Ala Pro Val Ala Glu Ser 1410 1415 1420 Val Thr Val Thr Asp Ser Asp Val Thr Ser Val Ala Ser Ser Ala Pro 1425 1430 1435 1440 Ser Ile Asp Leu Asn His Ile Gln Thr Val Met Met Asp Val Val Ala 1445 1450 1455 Asp Lys Thr Gly Tyr Pro Thr Asp Met Leu Glu Leu Gly Met Asp Met 1460 1465 1470 Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly 1475 1480 1485 Ala Val Gln Glu Ile Ile Thr Asp Leu Pro Glu Leu Asn Pro Glu Asp 1490 1495 1500 Leu Ala Glu Leu Arg Thr Leu Gly Glu Ile Val Ser Tyr Met Gln Ser 1505 1510 1515 1520 Lys Ala Pro Val Ala Glu Ala Pro Ala Ala Val Glu Ser Ala Pro Ile 1525 1530 1535 Gly Val Thr Ser Ser Ala Pro Ser Ile Asp Leu Asn His Ile Gln Thr 1540 1545 1550 Val Met Met Glu Val Val Ala Asp Lys Thr Gly Tyr Pro Val Asp Met 1555 1560 1565 Leu Glu Leu Ala Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile 1570 1575 1580 Lys Arg Val Glu Ile Leu Gly Ala Val Gln Glu Ile Ile Thr Asp Leu 1585 1590 1595 1600 Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Leu Arg Thr Leu Gly Glu 1605 1610 1615 Ile Val Ser Tyr Met Gln Ser Lys Val Pro Val Ala Glu Ala Pro Val 1620 1625 1630 Ala Val Glu Ser Ala His Thr Gly Val Thr Ser Ser Ala Pro Ser Ile 1635 1640 1645 Asp Leu Asp His Ile Gln Asn Val Met Met Asp Val Val Ala Asp Lys 1650 1655 1660 Thr Gly Tyr Pro Ala Asn Met Leu Glu Leu Ala Met Asp Met Glu Ala 1665 1670 1675 1680 Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly Ala Val 1685 1690 1695 Gln Glu Ile Ile Thr Asp Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala 1700 1705 1710 Glu Leu Arg Thr Leu Glu Glu Ile Val Thr Tyr Met Gln Ser Lys Ala 1715 1720 1725 Arg Gly Val Thr Ala Asn Val Val Ala Asn His Glu Pro Glu Lys Ser 1730 1735 1740 Ala Val Ser Asp Ala Phe Met Gln Asn Ser Val Ala Thr Ile Thr Ala 1745 1750 1755 1760 Ala Val Ala Pro Lys Ala Glu Phe Gln Pro Ala Pro Ser Ala Thr Val 1765 1770 1775 Ala Ile Ser Arg Leu Ser Ser Ile Ser Lys Ile Ser Gln Asn Cys Gln 1780 1785 1790 Gly Ala Asn Ala Leu Ile Val Ala Asp Gly Thr Asp Asn Ala Val Leu 1795 1800 1805 Leu Ala Asp His Leu Leu Gln Thr Gly Trp Asn Val Thr Ala Leu Gln 1810 1815 1820 Pro Thr Trp Val Ala Val Thr Thr Thr Lys Ala Phe Asn Gln Ser Val 1825 1830 1835 1840 Asn Leu Val Ser Leu Asn Gly Val Asp Glu Thr Glu Ile Asn Asn Ile 1845 1850 1855 Ile Ala Ala Asn Ala Gln Leu Asp Ala Val Ile Tyr Leu His Ala Ser 1860 1865 1870 Ser Glu Ile Asn Ala Ile Glu Tyr Pro Gln Ala Ser Lys Gln Gly Leu 1875 1880 1885 Met Leu Ala Phe Leu Leu Ala Lys Leu Ser Lys Val Thr Gln Ala Ala 1890 1895 1900 Lys Val Arg Gly Ser Phe Met Val Val Thr Gln Gln Gly Gly Ser Leu 1905 1910 1915 1920 Gly Phe Ser Asn Asp Ala Gln Ala Asp Leu Val Gln Ala Asp Leu Val 1925 1930 1935 Gln Thr Asp Leu Val Gln Ser Gly Leu Asn Gly Leu Val Lys Thr Leu 1940 1945 1950 Ser His Glu Trp Asp Asn Val Phe Cys Arg Ala Val Asp Met Pro Thr 1955 1960 1965 Ser Leu Ala Ala Ala Gln Val Ala Thr Leu Val Cys Asp Glu Leu Phe 1970 1975 1980 Asp Ala Asp Ser Val Leu Thr Glu Val Gly Tyr Gln Gln Ala Glu Gln 1985 1990 1995 2000 Asn Gly Val Ala Arg Ile Thr Leu Thr Gly Val Ala Thr Asp Ser Tyr 2005 2010 2015 Ala Leu Thr Ala Gly Asn Asn Ile Asp Ala Asn Ser Val Phe Leu Val 2020 2025 2030 Ser Gly Gly Ala Lys Gly Val Thr Ala His Cys Val Val Arg Ile Ala 2035 2040 2045 Glu Glu Tyr Gln Ala Lys Phe Ile Leu Leu Gly Arg Ser Lys Phe Ser 2050 2055 2060 Asn Asp Glu Pro Ser Trp Ala Asn Gly Ile Thr Glu Glu Ala Ala Leu 2065 2070 2075 2080 Lys Lys Ala Ala Met Gln Ala Leu Ile Ala Ala Gly Asp Lys Pro Thr 2085 2090 2095 Pro Val Lys Ile Val Gln Leu Ile Lys Pro Ile Gln Ala Asn Arg Glu 2100 2105 2110 Ile Ala Gln Thr Leu Ser Ala Ile Thr Ala Ala Gly Gly Gln Ala Glu 2115 2120 2125 Tyr Val Ser Ala Asp Val Thr Asn Ala Ala Ser Val Gln Thr Ala Val 2130 2135 2140 Thr Pro Ala Ile Ala Lys Phe Gly Ala Ile Thr Gly Ile Ile His Gly 2145 2150 2155 2160 Ala Gly Val Leu Ala Asp Gln Phe Ile Glu Gln Lys Thr Leu Ser Asp 2165 2170 2175 Phe Glu Ser Val Tyr Ser Thr Lys Ile Asp Gly Leu Leu Ser Leu Leu 2180 2185 2190 Ser Val Thr Glu Ala Ser Asn Ile Lys Gln Leu Val Leu Phe Ser Ser 2195 2200 2205 Ala Ala Gly Phe Tyr Gly Asn Pro Gly Gln Ser Asp Tyr Ser Ile Ala 2210 2215 2220 Asn Glu Ile Leu Asn Lys Thr Ala Tyr Arg Phe Lys Ser Leu His Pro 2225 2230 2235 2240 Gln Ala Gln Val Leu Ser Phe Asn Trp Gly Pro Trp Asp Gly Gly Met 2245 2250 2255 Val Thr Pro Glu Leu Lys Arg Met Phe Asp Gln Arg Gly Val Tyr Ile 2260 2265 2270 Ile Pro Leu Asp Ala Gly Ala Gln Leu Leu Leu Asn Glu Leu Ala Ala 2275 2280 2285 Asn Asp Asn Arg Cys Pro Gln Ile Leu Val Gly Asn Asp Leu Ser Lys 2290 2295 2300 Asp Ala Ala Asp Glu Lys Ser Thr Ala Val Lys Lys Pro Gln Val Ser 2305 2310 2315 2320 Arg Leu Ser Asp Ala Leu Val Thr Lys Ser Ile Lys Ala Thr Asn Ser 2325 2330 2335 Asp Ser Leu Asn Asp His Phe Gln Val Asn Asp Asn Arg Phe Leu Ala 2340 2345 2350 Asp His Met Ile Lys Gly Asn Gln Val Leu Pro Thr Val Cys Ala Ile 2355 2360 2365 Ala Trp Met Ser Asp Ala Ala Gln Ala Thr Tyr Ser Asn Arg Asp Cys 2370 2375 2380 Ala Leu Lys Tyr Val Gly Phe Glu Asp Tyr Lys Leu Phe Lys Gly Val 2385 2390 2395 2400 Val Phe Asp Gly Asn Glu Val Ala Asp Tyr Gln Ile Gln Leu Ser Pro 2405 2410 2415 Val Ala Ala Val Ser Glu His Glu Thr Val Val Arg Ile Ala Ala Lys 2420 2425 2430 Val Phe Ser Leu Lys Ser Asp Gly Lys Pro Val Phe His Tyr Ala Ala 2435 2440 2445 Thr Ile Leu Leu Ala Ser Gln Pro Leu Asp Ala Val Thr Leu Gln Leu 2450 2455 2460 Pro Val Leu Ala Val Asp Ile Asn Asn Leu Val Ser Asp Glu Ala Gln 2465 2470 2475 2480 Ala Leu Tyr Ser Asn Gly Thr Leu Phe His Gly Glu Ser Leu Gln Gly 2485 2490 2495 Ile Lys Gln Ile Leu Ser Cys Asp Asp Asn Gly Leu Leu Leu Ala Cys 2500 2505 2510 Gln Ile Thr Asp Val Ala Ala Ala Lys Gln Gly Ala Phe Pro Val Ala 2515 2520 2525 Ala Asn Asn Ile Phe Ala Asn Asp Leu Val Tyr Gln Ala Met Leu Val 2530 2535 2540 Trp Val Arg Lys Gln Phe Gly Leu Gly Ser Leu Pro Ser Val Thr Ala 2545 2550 2555 2560 Ala Trp Thr Val Tyr Arg Glu Val Ala Val Asp Glu Val Phe Tyr Leu 2565 2570 2575 Gln Leu Asn Val Val Glu His Asp Leu Leu Gly Ser Arg Gly Ser Lys 2580 2585 2590 Ala Arg Cys Asp Ile Gln Leu Ile Ala Ala Asp Met Gln Ile Leu Ala 2595 2600 2605 Glu Val Lys Ser Ala Gln Val Ser Val Ser Asp Ile Leu Asn Asp Met 2610 2615 2620 Ser 2625 <210> 3 <211> 2625 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 Met Ala Lys Lys Asn Thr Thr Ser Ile Lys His Ala Lys Asp Val Leu 1 5 10 15 Ser Ser Asp Asp Gln Gln Leu Asn Ser Arg Leu Gln Glu Cys Pro Ile 20 25 30 Ala Ile Ile Gly Met Ala Ser Val Phe Ala Asp Ala Lys Asn Leu Asp 35 40 45 Gln Phe Trp Asp Asn Ile Val Asp Ser Val Asp Ala Ile Ile Asp Val 50 55 60 Pro Ser Asp Arg Trp Asn Ile Asp Asp His Tyr Ser Ala Asp Lys Lys 65 70 75 80 Ala Ala Asp Lys Thr Tyr Cys Lys Arg Gly Gly Phe Ile Pro Glu Leu 85 90 95 Asp Phe Asp Pro Met Glu Phe Gly Leu Pro Pro Asn Ile Leu Glu Leu 100 105 110 Thr Asp Ile Ala Gln Leu Leu Ser Leu Ile Val Ala Arg Asp Val Leu 115 120 125 Ser Asp Ala Gly Ile Gly Ser Asp Tyr Asp His Asp Lys Ile Gly Ile 130 135 140 Thr Leu Gly Val Gly Gly Gly Gln Lys Gln Ile Ser Pro Leu Thr Ser 145 150 155 160 Arg Leu Gln Gly Pro Val Leu Glu Lys Val Leu Lys Ala Ser Gly Ile 165 170 175 Asp Glu Asp Asp Arg Ala Met Ile Ile Asp Lys Phe Lys Lys Ala Tyr 180 185 190 Ile Gly Trp Glu Glu Asn Ser Phe Pro Gly Met Leu Gly Asn Val Ile 195 200 205 Ala Gly Arg Ile Ala Asn Arg Phe Asp Phe Gly Gly Thr Asn Cys Val 210 215 220 Val Asp Ala Ala Cys Ala Gly Ser Leu Ala Ala Val Lys Met Ala Ile 225 230 235 240 Ser Asp Leu Leu Glu Tyr Arg Ser Glu Val Met Ile Ser Gly Gly Val 245 250 255 Cys Cys Asp Asn Ser Pro Phe Met Tyr Met Ser Phe Ser Lys Thr Pro 260 265 270 Ala Phe Thr Thr Asn Asp Asp Ile Arg Pro Phe Asp Asp Asp Ser Lys 275 280 285 Gly Met Leu Val Gly Glu Gly Ile Gly Met Met Ala Phe Lys Arg Leu 290 295 300 Glu Asp Ala Glu Arg Asp Gly Asp Lys Ile Tyr Ser Val Leu Lys Gly 305 310 315 320 Ile Gly Thr Ser Ser Asp Gly Arg Phe Lys Ser Ile Tyr Ala Pro Arg 325 330 335 Pro Asp Gly Gln Ala Lys Ala Leu Lys Arg Ala Tyr Glu Asp Ala Gly 340 345 345 350 Phe Ala Pro Glu Thr Cys Gly Leu Ile Glu Gly His Gly Thr Gly Thr 355 360 365 Lys Ala Gly Asp Ala Ala Glu Phe Ala Gly Leu Thr Lys His Phe Gly 370 375 380 380 Ala Ala Ser Asp Glu Lys Gln His Ile Ala Leu Gly Ser Val Lys Ser 385 390 395 400 Gln Ile Gly His Thr Lys Ser Ala Ala Gly Ser Ala Gly Met Ile Lys 405 410 415 Ala Ala Leu Ala Leu His His Lys Ile Leu Pro Ala Thr Ile His Ile 420 425 430 Asp Lys Pro Ser Glu Ala Leu Asp Ile Lys Asn Ser Pro Leu Tyr Leu 435 440 445 Asn Ser Glu Thr Arg Pro Trp Met Pro Arg Glu Asp Gly Ile Pro Arg 450 455 460 Arg Ala Gly Ile Ser Ser Phe Gly Phe Gly Gly Thr Asn Phe His Ile 465 470 475 480 Ile Leu Glu Glu Tyr Arg Pro Gly His Asp Ser Ala Tyr Arg Leu Asn 485 490 495 Ser Val Ser Gln Thr Val Leu Ile Ser Ala Asn Asp Gln Gln Gly Ile 500 505 510 Ile Ala Glu Leu Asn Asn Trp Arg Thr Lys Leu Ala Val Asp Ala Glu 515 520 525 Gln Gln Ala Phe Val Phe Asn Asp Leu Val Thr Thr Trp Pro Leu Lys 530 535 540 Thr Pro Ser Val Asn Gln Ala Arg Leu Gly Phe Val Ala Arg Asn Ala 545 550 555 560 Asn Glu Ala Ile Ala Met Ile Asp Thr Ala Leu Lys Gln Phe Asn Ala 565 570 575 Asn Ala Asp Lys Thr Thr Trp Ser Val Pro Thr Gly Val Tyr Tyr Arg 580 585 590 Gln Ala Gly Ile Asp Ala Thr Gly Lys Val Val Ala Leu Phe Ser Gly 595 600 605 Gln Gly Ser Gln Tyr Val Asn Met Gly Arg Glu Leu Thr Cys Asn Phe 610 615 620 Pro Ser Met Met Asp Ser Ala Ala Ala Met Asp Lys Glu Phe Ser Gly 625 630 635 640 Ala Gly Leu Gly Gln Leu Ser Ala Val Thr Phe Pro Ile Pro Val Tyr 645 650 655 Thr Asp Thr Glu Arg Lys Leu Gln Glu Asp Gln Leu Arg Leu Thr Gln 660 665 670 His Ala Gln Pro Ala Ile Gly Ser Leu Ser Val Gly Leu Phe Lys Thr 675 680 685 Phe Gln Gln Ala Gly Phe Lys Ala Asp Phe Ala Ala Gly His Ser Phe 690 695 700 Gly Glu Leu Thr Ala Leu Trp Ala Ala Asp Val Leu Ser Glu Ser Asp 705 710 715 720 Tyr Met Met Leu Ala Arg Ser Arg Gly Gln Ala Met Ala Ala Pro Glu 725 730 735 Gln Gln Asp Phe Asp Ala Gly Lys Met Ala Ala Val Val Gly Asp Pro 740 745 750 Lys Gln Val Ala Val Ile Ile Asp Thr Ile Asp Asp Val Ser Ile Ala 755 760 765 Asn Tyr Asn Ser Asn Asn Gln Val Val Ile Ala Gly Thr Thr Ala Gln 770 775 780 Val Ala Val Ala Val Thr Thr Leu Gly Asn Ala Gly Phe Lys Val Val 785 790 795 800 Pro Leu Pro Val Ser Ala Ala Phe His Thr Pro Leu Val Gly His Ala 805 810 81 5 Gln Lys Pro Phe Ala Lys Ala Val Asp Arg Ala Lys Phe Lys Ala Pro 820 825 830 Ser Ile Pro Val Phe Ala Asn Gly Thr Gly Leu Val His Ser Ser Lys 835 840 845 Pro Asn Asp Ile Lys Lys Asn Leu Lys Asn His Met Leu Glu Ser Val 850 855 860 His Phe Asn Gln Glu Ile Asp Asn Ile Tyr Ala Asp Gly Gly Arly Val 865 870 875 880 Phe Ile Glu Phe Gly Pro Lys Asn Val Leu Thr Lys Leu Val Glu Asn 885 890 895 Ile Leu Thr Asp Lys Ser Asp Val Thr Ala Ile Ala Val Asn Ala Asn 900 905 910 Pro Lys Gln Pro Ala Asp Val Gln Met Arg Gln Ala Ala Leu Gln Met 915 920 925 Ala Val Leu Gly Val Ala Leu Asp Asn Ile Asp Pro Tyr Asp Ala Val 930 935 940 Lys Arg Pro Leu Val Ala Pro Lys Ala Ser Pro Met Leu Met Lys Leu 945 950 955 960 Ser Ala Ala Ser Tyr Val Ser Pro Lys Thr Lys Lys Ala Phe Ala Asp 965 970 975 Ala Leu Thr Asp Gly Trp Thr Val Lys Gln Ala Lys Ala Ala Pro Val 980 985 990 Val Val Ser Gln Pro Gln Val Ile Glu Lys Ile Val Glu Val Glu Lys 995 1000 1005 Ile Val Glu Arg Ile Val Glu Val Glu Arg Ile Val Glu Val Glu Lys 1010 1015 10 20 Ile Val Tyr Val Asn Ala Asp Gly Ser His Ala Thr Thr Asn Asn Ser 1025 1030 1035 1040 Ala Ile Asn Asn Gly Met His Ser Ser Asp Ala Asp Leu Val Ala Ser 1045 1050 1055 Ile Glu Gln Ser Val Gly Gln Phe Val Ala His Gln Gln Gln Leu Leu 1060 1065 1070 Asn Val His Glu Gln Phe Met Gln Gly Pro Gln Asp Tyr Ala Lys Thr 1075 1080 1085 Val Gln Asn Val Leu Ala Ala Gln Thr Asn Asn Glu Leu Pro Glu Ser 1090 1095 1100 Leu Asp Arg Thr Leu Ser Met Tyr Asn Glu Phe Gln Ser Glu Thr Leu 1105 1110 1115 1120 Arg Val His Glu Thr Tyr Leu Asn Asn Gln Thr Ser Ser Met Asn Thr 1125 1130 1135 Met Leu Thr Gly Ala Glu Thr Asp Val Leu Val Ala Pro Val Thr Gln 1140 1145 1150 Val Leu Asn Thr Pro Val Ala Ala Ser His Lys Ala Val Ala Pro Val 1155 1160 1165 Ile Ala Asn Thr Val Thr Asn Val Val Ser Ser Val Ser Asn Asn Ala 1170 1175 1180 Ala Val Ala Val Gln Thr Val Ala Leu Ala Pro Thr Gln Glu Ile Ala 1185 1190 1195 1200 Pro Ala Val Val Thr Thr Pro Ala Pro Ala Leu Val Ala Ile Val Ala 1205 1210 1215 Glu Pro Val Ile Val Ala Pro Val Al a Thr Glu Val Ala Pro Ile Thr 1220 1225 1230 Ser Ser Val Thr Pro Val Val Ala Thr Gln Ala Ala Ile Asp Val Ala 1235 1240 1245 Thr Ile Asn Lys Val Met Leu Glu Val Val Ala Asp Lys Thr Gly Tyr 1250 1255 1260 Pro Thr Asp Met Leu Glu Leu Ser Met Asp Met Glu Ala Asp Leu Gly 1265 1270 1275 1280 Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly Ala Val Gln Glu Leu 1285 1290 1295 Ile Pro Asp Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Leu Arg 1300 1305 1310 Thr Leu Gly Glu Ile Val Asp Tyr Met Asn Val Lys Ala Gln Glu Ala 1315 1320 1325 Ala Pro Val Ser Ala Pro Ala Ser Ala Gly Ile Asp Leu Ala His Ile 1330 1335 1340 Gln Asn Val Met Leu Glu Val Val Ala Asp Lys Thr Gly Tyr Pro Thr 1345 1350 1355 1360 Asp Met Leu Glu Leu Ser Met Asp Met Glu Ala Asp Leu Gly Ile Asp 1365 1370 1375 Ser Ile Lys Arg Val Glu Ile Leu Gly Ala Val Gln Glu Ile Ile Thr 1380 1385 1390 Asp Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Leu Arg Thr Leu 1395 1400 1405 Gly Glu Ile Val Ser Tyr Met Gln Ser Lys Ala Pro Val Ala Glu Ser 1410 1415 14 20 Val Thr Val Thr Asp Ser Asp Val Thr Ser Val Ala Ser Ser Ala Pro 1425 1430 1435 1440 Ser Ile Asp Leu Asn His Ile Gln Thr Val Met Met Asp Val Val Ala 1445 1450 1455 Asp Lys Thr Gly Tyr Pro Thr Asp Met Leu Glu Leu Gly Met Asp Met 1460 1465 1470 Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly 1475 1480 1485 Ala Val Gln Glu Ile Ile Thr Asp Leu Pro Glu Leu Asn Pro Glu Asp 1490 1495 1500 Leu Ala Glu Leu Arg Thr Leu Gly Glu Ile Val Ser Tyr Met Gln Ser 1505 1510 1515 1520 Lys Ala Pro Val Ala Glu Ala Pro Ala Ala Val Glu Ser Ala Pro Ile 1525 1530 1535 Gly Val Thr Ser Ser Ala Pro Ser Ile Asp Leu Asn His Ile Gln Thr 1540 1545 1550 Val Met Met Glu Val Val Ala Asp Lys Thr Gly Tyr Pro Val Asp Met 1555 1560 1565 Leu Glu Leu Ala Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile 1570 1575 1580 Lys Arg Val Glu Ile Leu Gly Ala Val Gln Glu Ile Ile Thr Asp Leu 1585 1590 1595 1600 Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Leu Arg Thr Leu Gly Glu 1605 1610 1615 Ile Val Ser Tyr Met Gln Ser Lys Va l Pro Val Ala Glu Ala Pro Val 1620 1625 1630 Ala Val Glu Ser Ala His Thr Gly Val Thr Ser Ser Ala Pro Ser Ile 1635 1640 1645 Asp Leu Asp His Ile Gln Asn Val Met Met Asp Val Val Ala Asp Lys 1650 1655 1660 Thr Gly Tyr Pro Ala Asn Met Leu Glu Leu Ala Met Asp Met Glu Ala 1665 1670 1675 1680 Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly Ala Val 1685 1690 1695 Gln Glu Ile Ile Thr Asp Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala 1700 1705 1710 Glu Leu Arg Thr Leu Glu Glu Ile Val Thr Tyr Met Gln Ser Lys Ala 1715 1720 1725 Arg Gly Val Thr Ala Asn Val Val Ala Asn His Glu Pro Glu Lys Ser 1730 1735 1740 Ala Val Ser Asp Ala Phe Met Gln Asn Ser Val Ala Thr Ile Thr Ala 1745 1750 1755 1760 Ala Val Ala Pro Lys Ala Glu Phe Gln Pro Ala Pro Ser Ala Thr Val 1765 1770 1775 Ala Ile Ser Arg Leu Ser Ser Ile Ser Lys Ile Ser Gln Asn Cys Gln 1780 1785 1790 Gly Ala Asn Ala Leu Ile Val Ala Asp Gly Thr Asp Asn Ala Val Leu 1795 1800 1805 Leu Ala Asp His Leu Leu Gln Thr Gly Trp Asn Val Thr Ala Leu Gln 1810 1815 18 20 Pro Thr Trp Val Ala Val Thr Thr Thr Lys Ala Phe Asn Gln Ser Val 1825 1830 1835 1840 Asn Leu Val Ser Leu Asn Gly Val Asp Glu Thr Glu Ile Asn Asn Ile 1845 1850 1855 Ile Ala Ala Asn Ala Gln Leu Asp Ala Val Ile Tyr Leu His Ala Ser 1860 1865 1870 Ser Glu Ile Asn Ala Ile Glu Tyr Pro Gln Ala Ser Lys Gln Gly Leu 1875 1880 1885 Met Leu Ala Phe Leu Leu Ala Lys Leu Ser Lys Val Thr Gln Ala Ala 1890 1895 1900 Lys Val Arg Gly Ser Phe Met Val Val Thr Gln Gln Gly Gly Ser Leu 1905 1910 1915 1920 Gly Phe Ser Asn Asp Ala Gln Ala Asp Leu Val Gln Ala Asp Leu Val 1925 1930 1935 Gln Thr Asp Leu Val Gln Ser Gly Leu Asn Gly Leu Val Lys Thr Leu 1940 1945 1950 Ser His Glu Trp Asp Asn Val Phe Cys Arg Ala Val Asp Met Pro Thr 1955 1960 1965 Ser Leu Ala Ala Ala Gln Val Ala Thr Leu Val Cys Asp Glu Leu Phe 1970 1975 1980 Asp Ala Asp Ser Val Leu Thr Glu Val Gly Tyr Gln Gln Ala Glu Gln 1985 1990 1995 2000 Asn Gly Val Ala Arg Ile Thr Leu Thr Gly Val Ala Thr Asp Ser Tyr 2005 2010 2015 Ala Leu Thr Ala Gly Asn Asn Ile As p Ala Asn Ser Val Phe Leu Val 2020 2025 2030 Ser Gly Gly Ala Lys Gly Val Thr Ala His Cys Val Val Arg Ile Ala 2035 2040 2045 Glu Glu Tyr Gln Ala Lys Phe Ile Leu Leu Gly Arg Ser Lys Phe Ser 2050 2055 2060 Asn Asp Glu Pro Ser Trp Ala Asn Gly Ile Thr Glu Glu Ala Ala Leu 2065 2070 2075 2080 Lys Lys Ala Ala Met Gln Ala Leu Ile Ala Ala Ala Gly Asp Lys Pro Thr 2085 2090 2095 Pro Val Lys Ile Val Gln Leu Ile Lys Pro Ile Gln Ala Asn Arg Glu 2100 2105 2110 Ile Ala Gln Thr Leu Ser Ala Ile Thr Ala Ala Gly Gly Gln Ala Glu 2115 2120 2125 Tyr Val Ser Ala Asp Val Thr Asn Ala Ala Ser Val Gln Thr Ala Val 2130 2135 2140 Thr Pro Ala Ile Ala Lys Phe Gly Ala Ile Thr Gly Ile Ile His Gly 2145 2150 2155 2160 Ala Gly Val Leu Ala Asp Gln Phe Ile Glu Gln Lys Thr Leu Ser Asp 2165 2170 2175 Phe Glu Ser Val Tyr Ser Thr Lys Ile Asp Gly Leu Leu Ser Leu Leu 2180 2185 2190 Ser Val Thr Glu Ala Ser Asn Ile Lys Gln Leu Val Leu Phe Ser Ser 2195 2200 2205 Ala Ala Gly Phe Tyr Gly Asn Pro Gly Gln Ser Asp Tyr Ser Ile Ala 2210 2215 22 20 Asn Glu Ile Leu Asn Lys Thr Ala Tyr Arg Phe Lys Ser Leu His Pro 2225 2230 2235 2240 Gln Ala Gln Val Leu Ser Phe Asn Trp Gly Pro Trp Asp Gly Gly Met 2245 2250 2255 Val Thr Pro Glu Leu Lys Arg Met Phe Asp Gln Arg Gly Val Tyr Ile 2260 2265 2270 Ile Pro Leu Asp Ala Gly Ala Gln Leu Leu Leu Asn Glu Leu Ala Ala 2275 2280 2285 Asn Asp Asn Arg Cys Pro Gln Ile Leu Val Gly Asn Asp Leu Ser Lys 2290 2295 2300 Asp Ala Ala Asp Glu Lys Ser Thr Ala Val Lys Lys Pro Gln Val Ser 2305 2310 2315 2320 Arg Leu Ser Asp Ala Leu Val Thr Lys Ser Ile Lys Ala Thr Asn Ser 2325 2330 2335 Asp Ser Leu Asn Asp His Phe Gln Val Asn Asp Asn Arg Phe Leu Ala 2340 2345 2350 Asp His Met Ile Lys Gly Asn Gln Val Leu Pro Thr Val Cys Ala Ile 2355 2360 2365 Ala Trp Met Ser Asp Ala Ala Gla Aln Thr Glu Asp Tyr Lys Leu Phe Lys Gly Val 2385 2390 2395 2400 Val Phe Asp Gly Asn Glu Val Ala Asp Tyr Gln Ile Gln Leu Ser Pro 2405 2410 2415 Val Ala Ala Val Ser Glu His Glu Th r Val Val Arg Ile Ala Ala Lys 2420 2425 2430 Val Phe Ser Leu Lys Ser Asp Gly Lys Pro Val Phe His Tyr Ala Ala 2435 2440 2445 Thr Ile Leu Leu Ala Ser Gln Pro Leu Asp Ala Val Thr Leu Gln Leu 2450 2455 2460 Pro Val Leu Ala Val Asp Ile Asn Asn Leu Val Ser Asp Glu Ala Gln 2465 2470 2475 2480 Ala Leu Tyr Ser Asn Gly Thr Leu Phe His Gly Glu Ser Leu Gln Gly 2485 2490 2495 Ile Lys Gln Ile Leu Ser Cys Asp Asp Asn Gly Leu Leu Leu Ala Cys 2500 2505 2510 Gln Ile Thr Asp Val Ala Ala Ala Lys Gln Gly Ala Phe Pro Val Ala 2515 2520 2525 Ala Asn Asn Ile Phe Ala Asn Asp Leu Val Tyr Gln Ala Met Leu Val 2530 2535 2540 Trp Val Arg Lys Gln Phe Gly Leu Gly Ser Leu Pro Ser Val Thr Ala 2545 2550 2555 2560 Ala Trp Thr Val Tyr Arg Glu Val Ala Val Asp Glu Val Phe Tyr Leu 2565 2570 2575 Gln Leu Asn Val Val Glu His Asp Leu Leu Gly Ser Arg Gly Ser Lys 2580 2585 2590 Ala Arg Cys Asp Ile Gln Leu Ile Ala Ala Asp Met Gln Ile Leu Ala 2595 2600 2605 Glu Val Lys Ser Ala Gln Val Ser Val Ser Asp Ile Leu Asn Asp Met 2610 2615 26 20 Ser 2625

【００４６】 <210> 4 <211> 2631 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 atgacggaat tagctgttat tggtatggat gctaaattta gcggacagga caatattgat 60 cgtgtggaac gtgctttata tcaaggtgct tatgtagata atgttagccg cgttagtatc 120 gactctaacg ttattagcta tggcgaagaa caagttatta ctgccgcgac agtacttaac 180 tctgtcggtc tactagcgca aacaaatcag ttaaacatgg cgaatatagc ggtattgctt 240 attgctgata tgagcagtga tgttgataac cagcttatag cgcaaattgc agcagaaatt 300 gaaaaacagt gtgcgtgttg tattgttatt gctgatttag gccaagcatt aaagcaagta 360 acagatttgg ttaataacca agactgtcct gtggctgtga ttggtatgaa taacctagtt 420 catgcgccta gccatgatat tgaagctgcg gcaacagcca ctgcaacaat aagctttgat 480 gaaaccttca ttggttatag cagtgtagct gggttcgcta gtttacttat cgcttcaaca 540 acgtttgcca atgctaagca gtgttatata tacgccaaca ttaagggctt tgctcagtct 600 ggtgtcagtg ctcaatttaa cgctgcaaac attaccgata ctgcaaacat tgcattacag 660 caagctggca tgaatgcaga gcaggttggt ttgttagaag tttcagcagt cgctgattcg 720 gcaattgcat tgtctgaaag ccaaggttta atgtctgctt ataaccatac acaaacttta 780 cataccgcat taagtagtgc ccgtagtgtg actggcgaag gcggttgctt ctcgcaggtt 840 gcagggttat tgaaatgtgt gatcagtttg catcagcgtt atattccggc gattaaagat 900 tggcaacagc cgagtgatac gcaaatgtca caatggcttc actcaccatt ttatatgcct 960 gttgatgctc gaccttggtt tccacatgct gatggctctg cacacgttgc ggcttatagt 1020 tgtgtatctg ctaacagcta ttgtcacatt cttttacaag aaaatgactt aactgaaagc 1080 aagcttcaaa cacttgcaca aaaccatcca gtagctgatc tgcgcactaa tggttacttt 1140 gcatcgagcg agttagcact agttatagtg caaggtaata acgaagctca attacgcagt 1200 gaattagaga ctattgctgg gcagttaagt actactggta taaatactgc cgatattaaa 1260 cagatcgcgg ctgattgtta tgcccgaaat gatacggaca aagcctatag cgcagtactt 1320 attgccgata ctgctgaaga gttaagcaaa gagatcactt tagcgtttac tggtattgct 1380 agcgtgttta atgacgatgc taaagaatgg aaaaccccga agggtagtta ctttactgcg 1440 tcgccagcta acaaagacgc tgcaaaccca gattcaaata acagcacaca gaatggcgtc 1500 acctttatgt acccaggtat tggcgctacg tatgttggtt tagggcgtga tctatttcat 1560 ctattcccac agatttatca gcctgtagcg gctttagccg atgacattgc tgccactcta 1620 aaagacacct tacttaatcc acgcagcatt agccgttata gctttaaaga actcaagcag 1680 ctggatctgg atctacgcgg taatttagct aatatcgctg aagccggtgt gggttttgct 1740 tgcgtattta ccaaggtatt tgaagaagta tttgccgtta aagctgactt tgctacaggt 1800 tatagcatgg gtgaagtaag catgtatgcg gcactaggct gctggcaaca accgggattg 1860 atgagtgctc gtcttgcaca atcgaatacc tttaatcatc aactttgcgg cgagttaaga 1920 acactacgtc agcattgggg gatggatgat gttgctaacg gtacgttcga gcagatctgg 1980 gaaacgtata ccattaaggc aacgattgaa caggttgaaa ttgcctctgc agatgaagac 2040 cgtgtgtatt gcaccattat caatacgcct gatagcttac tgttagcggg ttatccagaa 2100 gcgtgtcagc gagtcattaa gaatttaggt gtgcgcgcaa tggcattaaa catggcgaac 2160 gcgattcaca gcgcgccagc ttatgccgaa tacgatcata tggttgagtt ataccatatg 2220 gacgttaccc cgcgtattaa taccaagatg tattcaagct catgttattt acctattcca 2280 caacgcagca aagcgatttc acacagtatt gcgaaatgtt tgtgtgatgt agtggatttc 2340 ccacgtttgg ttaatacctt acatgacaaa ggtgcgcgag tattcattga aatgggtcca 2400 ggtcgttcgt tatgtagctg ggtagataag atcctagtta ataacgatgg cgataatcaa 2460 aagcaaagcc aacatgtatc tgtgcctgtg aatgccaaag gcaccagtga tgaacttact 2520 tatattcgtg cgattgctaa gttaattagt catggcgtga atttgaattt agatagcttg 2580 tttaacgggt caatcctggt taaagcaggc cgtatagaaa atacaaataa a 2631<210> 4 <211> 2631 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 atgacggaat tagctgttat tggtatggat gctaaattta gcggacagga caatattgat 60 cgtgtggaac gtgctttata tcaaggtg ctg ctagg tatg ctag gc tatg ctag gc tatg tatg ctag gc tatg ctag gc tggcgaagaa caagttatta ctgccgcgac agtacttaac 180 tctgtcggtc tactagcgca aacaaatcag ttaaacatgg cgaatatagc ggtattgctt 240 attgctgata tgagcagtga tgttgataac cagcttatag cgcaaattgc agcagaaatt 300 gaaaaacagt gtgcgtgttg tattgttatt gctgatttag gccaagcatt aaagcaagta 360 acagatttgg ttaataacca agactgtcct gtggctgtga ttggtatgaa taacctagtt 420 catgcgccta gccatgatat tgaagctgcg gcaacagcca ctgcaacaat aagctttgat 480 gaaaccttca ttggttatag cagtgtagct gggttcgcta gtttacttat cgcttcaaca 540 acgtttgcca atgctaagca gtgttatata tacgccaaca ttaagggctt tgctcagtct 600 ggtgtcagtg ctcaatttaa cgctgcaaac attaccgata ctgcaaacat tgcattacag 660 caagctggca tgaatgcaga gcaggttggt ttgttagaag tttcag cgctgatcag atcagtcagtcagtcagtcagtcagtcagtcagtcagtcagtcagtcagtcagtcagtcag acttta 780 cataccgcat taagtagtgc ccgtagtgtg actggcgaag gcggttgctt ctcgcaggtt 840 gcagggttat tgaaatgtgt gatcagtttg catcagcgtt atattccggc gattaaagat 900 tggcaacagc cgagtgatac gcaaatgtca caatggcttc actcaccatt ttatatgcct 960 gttgatgctc gaccttggtt tccacatgct gatggctctg cacacgttgc ggcttatagt 1020 tgtgtatctg ctaacagcta ttgtcacatt cttttacaag aaaatgactt aactgaaagc 1080 aagcttcaaa cacttgcaca aaaccatcca gtagctgatc tgcgcactaa tggttacttt 1140 gcatcgagcg agttagcact agttatagtg caaggtaata acgaagctca attacgcagt 1200 gaattagaga ctattgctgg gcagttaagt actactggta taaatactgc cgatattaaa 1260 cagatcgcgg ctgattgtta tgcccgaaat gatacggaca aagcctatag cgcagtactt 1320 attgccgata ctgctgaaga gttaagcaaa gagatcactt tagcgtttac tggtattgct 1380 agcgtgttta atgacgatgc taaagaatgg aaaaccccga agggtagtta ctttactgcg 1440 tcgccagcta acaaagacgc tgcaaaccca gattcaaata acagcacaca gaatggcgtc 1500 acctttatgt acccaggtat tggcgctacg tatgttggtt tagggcgtga tctatttcat 1560 ctattcccac agatttatca gcctgtagcg gctttagccg atgacattgc tgccactcta 162 0 aaagacacct tacttaatcc acgcagcatt agccgttata gctttaaaga actcaagcag 1680 ctggatctgg atctacgcgg taatttagct aatatcgctg aagccggtgt gggttttgct 1740 tgcgtattta ccaaggtatt tgaagaagta tttgccgtta aagctgactt tgctacaggt 1800 tatagcatgg gtgaagtaag catgtatgcg gcactaggct gctggcaaca accgggattg 1860 atgagtgctc gtcttgcaca atcgaatacc tttaatcatc aactttgcgg cgagttaaga 1920 acactacgtc agcattgggg gatggatgat gttgctaacg gtacgttcga gcagatctgg 1980 gaaacgtata ccattaaggc aacgattgaa caggttgaaa ttgcctctgc agatgaagac 2040 cgtgtgtatt gcaccattat caatacgcct gatagcttac tgttagcggg ttatccagaa 2100 gcgtgtcagc gagtcattaa gaatttaggt gtgcgcgcaa tggcattaaa catggcgaac 2160 gcgattcaca gcgcgccagc ttatgccgaa tacgatcata tggttgagtt ataccatatg 2220 gacgttaccc cgcgtattaa taccaagatg tattcaagct catgttattt acctattcca 2280 caacgcagca aagcgatttc acacagtatt gcgaaatgtt tgtgtgatgt agtggatttc 2340 ccacgtttgg ttaatacctt acatgacaaa ggtgcgcgag tattcattga aatgggtcca 2400 ggtcgttcgt tatgtagctg ggtagataag atcctagtta ataacgatgg cgataatcaa 2460 aagc aaagcc aacatgtatc tgtgcctgtg aatgccaaag gcaccagtga tgaacttact 2520 tatattcgtg cgattgctaa gttaattagt catggcgtga atttgaattt agatagcttg 2580 tttaacgggt caatcctggt taaagcaggc cgtatagaaa atacaaata

【００４７】 <21> 5 <211> 877 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 Met Thr Glu Leu Ala Val Ile Gly Met Asp Ala Lys Phe Ser Gly Gln 1 5 10 15 Asp Asn Ile Asp Arg Val Glu Arg Ala Leu Tyr Gln Gly Ala Tyr Val 20 25 30 Asp Asn Val Ser Arg Val Ser Ile Asp Ser Asn Val Ile Ser Tyr Gly 35 40 45 Glu Glu Gln Val Ile Thr Ala Ala Thr Val Leu Asn Ser Val Gly Leu 50 55 60 Leu Ala Gln Thr Asn Gln Leu Asn Met Ala Asn Ile Ala Val Leu Leu 65 70 75 80 Ile Ala Asp Met Ser Ser Asp Val Asp Asn Gln Leu Ile Ala Gln Ile 85 90 95 Ala Ala Glu Ile Glu Lys Gln Cys Ala Cys Cys Ile Val Ile Ala Asp 100 105 110 Leu Gly Gln Ala Leu Lys Gln Val Thr Asp Leu Val Asn Asn Gln Asp 115 120 125 Cys Pro Val Ala Val Ile Gly Met Asn Asn Leu Val His Ala Pro Ser 130 135 140 His Asp Ile Glu Ala Ala Ala Thr Ala Thr Ala Thr Ile Ser Phe Asp 145 150 155 160 Glu Thr Phe Ile Gly Tyr Ser Ser Val Ala Gly Phe Ala Ser Leu Leu 165 170 175 Ile Ala Ser Thr Thr Phe Ala Asn Ala Lys Gln Cys Tyr Ile Tyr Ala 180 185 190 Asn Ile Lys Gly Phe Ala Gln Ser Gly Val Ser Ala Gln Phe Asn Ala 195 200 205 Ala Asn Ile Thr Asp Thr Ala Asn Ile Ala Leu Gln Gln Ala Gly Met 210 215 220 Asn Ala Glu Gln Val Gly Leu Leu Glu Val Ser Ala Val Ala Asp Ser 225 230 235 240 Ala Ile Ala Leu Ser Glu Ser Gln Gly Leu Met Ser Ala Tyr Asn His 245 250 255 Thr Gln Thr Leu His Thr Ala Leu Ser Ser Ala Arg Ser Val Thr Gly 260 265 270 Glu Gly Gly Cys Phe Ser Gln Val Ala Gly Leu Leu Lys Cys Val Ile 275 280 285 Ser Leu His Gln Arg Tyr Ile Pro Ala Ile Lys Asp Trp Gln Gln Pro 290 295 300 Ser Asp Thr Gln Met Ser Gln Trp Leu His Ser Pro Phe Tyr Met Pro 305 310 315 320 Val Asp Ala Arg Pro Trp Phe Pro His Ala Asp Gly Ser Ala His Val 325 330 335 Ala Ala Tyr Ser Cys Val Ser Ala Asn Ser Tyr Cys His Ile Leu Leu 340 345 350 Gln Glu Asn Asp Leu Thr Glu Ser Lys Leu Gln Thr Leu Ala Gln Asn 355 360 365 His Pro Val Ala Asp Leu Arg Thr Asn Gly Tyr Phe Ala Ser Ser Glu 370 375 380 Leu Ala Leu Val Ile Val Gln Gly Asn Asn Glu Ala Gln Leu Arg Ser 385 390 395 400 Glu Leu Glu Thr Ile Ala Gly Gln Leu Ser Thr Thr Gly Ile Asn Thr 405 410 415 Ala Asp Ile Lys Gln Ile Ala Ala Asp Cys Tyr Ala Arg Asn Asp Thr 420 425 430 Asp Lys Ala Tyr Ser Ala Val Leu Ile Ala Asp Thr Ala Glu Glu Leu 435 440 445 Ser Lys Glu Ile Thr Leu Ala Phe Thr Gly Ile Ala Ser Val Phe Asn 450 455 460 Asp Asp Ala Lys Glu Trp Lys Thr Pro Lys Gly Ser Tyr Phe Thr Ala 465 470 475 480 Ser Pro Ala Asn Lys Asp Ala Ala Asn Pro Asp Ser Asn Asn Ser Thr 485 490 495 Gln Asn Gly Val Thr Phe Met Tyr Pro Gly Ile Gly Ala Thr Tyr Val 500 505 510 Gly Leu Gly Arg Asp Leu Phe His Leu Phe Pro Gln Ile Tyr Gln Pro 515 520 525 Val Ala Ala Leu Ala Asp Asp Ile Ala Ala Thr Leu Lys Asp Thr Leu 530 535 540 Leu Asn Pro Arg Ser Ile Ser Arg Tyr Ser Phe Lys Glu Leu Lys Gln 545 550 555 560 Leu Asp Leu Asp Leu Arg Gly Asn Leu Ala Asn Ile Ala Glu Ala Gly 565 570 575 Val Gly Phe Ala Cys Val Phe Thr Lys Val Phe Glu Glu Val Phe Ala 580 585 590 Val Lys Ala Asp Phe Ala Thr Gly Tyr Ser Met Gly Glu Val Ser Met 595 600 605 Tyr Ala Ala Leu Gly Cys Trp Gln Gln Pro Gly Leu Met Ser Ala Arg 610 615 620 Leu Ala Gln Ser Asn Thr Phe Asn His Gln Leu Cys Gly Glu Leu Arg 625 630 635 640 Thr Leu Arg Gln His Trp Gly Met Asp Asp Val Ala Asn Gly Thr Phe 645 650 655 Glu Gln Ile Trp Glu Thr Tyr Thr Ile Lys Ala Thr Ile Glu Gln Val 660 665 670 Glu Ile Ala Ser Ala Asp Glu Asp Arg Val Tyr Cys Thr Ile Ile Asn 675 680 685 Thr Pro Asp Ser Leu Leu Leu Ala Gly Tyr Pro Glu Ala Cys Gln Arg 690 695 700 Val Ile Lys Asn Leu Gly Val Arg Ala Met Ala Leu Asn Met Ala Asn 705 710 715 720 Ala Ile His Ser Ala Pro Ala Tyr Ala Glu Tyr Asp His Met Val Glu 725 730 735 Leu Tyr His Met Asp Val Thr Pro Arg Ile Asn Thr Lys Met Tyr Ser 740 745 750 Ser Ser Cys Tyr Leu Pro Ile Pro Gln Arg Ser Lys Ala Ile Ser His 755 760 765 Ser Ile Ala Lys Cys Leu Cys Asp Val Val Asp Phe Pro Arg Leu Val 770 775 780 Asn Thr Leu His Asp Lys Gly Ala Arg Val Phe Ile Glu Met Gly Pro 785 790 795 800 Gly Arg Ser Leu Cys Ser Trp Val Asp Lys Ile Leu Val Asn Asn Asp 805 810 815 Gly Asp Asn Gln Lys Gln Ser Gln His Val Ser Val Pro Val Asn Ala 820 825 830 Lys Gly Thr Ser Asp Glu Leu Thr Tyr Ile Arg Ala Ile Ala Lys Leu 835 840 845 Ile Ser His Gly Val Asn Leu Asn Leu Asp Ser Leu Phe Asn Gly Ser 850 855 860 Ile Leu Val Lys Ala Gly Arg Ile Glu Asn Thr Asn Lys 865 870 875 <21> 5 <211> 877 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 Met Thr Glu Leu Ala Val Ile Gly Met Asp Ala Lys Phe Ser Gly Gln 1 5 10 15 Asp Asn Ile Asp Arg Val Glu Arg Ala Leu Tyr Gln Gly Ala Tyr Val 20 25 30 Asp Asn Val Ser Arg Val Ser Ile Asp Ser Asn Val Ile Ser Tyr Gly 35 40 45 Glu Glu Glu Val Ile Thr Ala Ala Thr Val Leu Asn Ser Val Gly Leu 50 55 60 Leu Ala Gln Thr Asn Gln Leu Asn Met Ala Asn Ile Ala Val Leu Leu 65 70 75 80 Ile Ala Asp Met Ser Ser Asp Val Asp Asn Gln Leu Ile Ala Gln Ile 85 90 95 Ala Ala Glu Ile Glu Lys Gln Cys Ala Cys Cys Ile Val Ile Ala Asp 100 105 110 Leu Gly Gln Ala Leu Lys Gln Val Thr Asp Leu Val Asn Asn Gln Asp 115 120 125 Cys Pro Val Ala Val Ile Gly Met Asn Asn Leu Val His Ala Pro Ser 130 135 140 His Asp Ile Glu Ala Ala Ala Thr Ala Thr Ala Thr Ile Ser Phe Asp 145 150 155 160 Glu Thr Phe Ile Gly Tyr Ser Ser Val Ala Gly Phe Ala Ser Leu Leu 165 170 175 Ile Ala Ser Thr Thr Phe Ala Asn Ala Lys Gln Cys Tyr Ile Tyr Ala 180 185 19 0 Asn Ile Lys Gly Phe Ala Gln Ser Gly Val Ser Ala Gln Phe Asn Ala 195 200 205 Ala Asn Ile Thr Asp Thr Ala Asn Ile Ala Leu Gln Gln Ala Gly Met 210 215 220 Asn Ala Glu Gln Val Gly Leu Leu Glu Val Ser Ala Val Ala Asp Ser 225 230 235 240 Ala Ile Ala Leu Ser Glu Ser Gln Gly Leu Met Ser Ala Tyr Asn His 245 250 255 Thr Gln Thr Leu His Thr Ala Leu Ser Ser Ala Arg Ser Val Thr Gly 260 265 270 Glu Gly Gly Cys Phe Ser Gln Val Ala Gly Leu Leu Lys Cys Val Ile 275 280 285 Ser Leu His Gln Arg Tyr Ile Pro Ala Ile Lys Asp Trp Gln Gln Pro 290 295 300 Ser Asp Thr Gln Met Ser Gln Trp Leu His Ser Pro Phe Tyr Met Pro 305 310 315 320 Val Asp Ala Arg Pro Trp Phe Pro His Ala Asp Gly Ser Ala His Val 325 330 335 Ala Ala Tyr Ser Cys Val Ser Ala Asn Ser Tyr Cys His Ile Leu Leu 340 345 350 Gln Glu Asn Asp Leu Thr Glu Ser Lys Leu Gln Thr Leu Ala Gln Asn 355 360 365 His Pro Val Ala Asp Leu Arg Thr Asn Gly Tyr Phe Ala Ser Ser Glu 370 375 380 Leu Ala Leu Val Ile Val Gln Gly Asn Asn Glu Ala Gln Leu Arg Ser 385 390 395 Four 00 Glu Leu Glu Thr Ile Ala Gly Gln Leu Ser Thr Thr Gly Ile Asn Thr 405 410 415 Ala Asp Ile Lys Gln Ile Ala Ala Asp Cys Tyr Ala Arg Asn Asp Thr 420 425 430 Asp Lys Ala Tyr Ser Ala Val Leu Ile Ala Asp Thr Ala Glu Glu Leu 435 440 445 Ser Lys Glu Ile Thr Leu Ala Phe Thr Gly Ile Ala Ser Val Phe Asn 450 455 460 Asp Asp Ala Lys Glu Trp Lys Thr Pro Lys Gly Ser Tyr Phe Thr Ala 465 470 475 480 Ser Pro Ala Asn Lys Asp Ala Ala Asn Pro Asp Ser Asn Asn Ser Thr 485 490 495 Gln Asn Gly Val Thr Phe Met Tyr Pro Gly Ile Gly Ala Thr Tyr Val 500 505 510 Gly Leu Gly Arg Asp Leu Phe His Leu Phe Pro Gln Ile Tyr Gln Pro 515 520 525 Val Ala Ala Leu Ala Asp Asp Ile Ala Ala Thr Leu Lys Asp Thr Leu 530 535 540 Leu Asn Pro Arg Ser Ile Ser Arg Tyr Ser Phe Lys Glu Leu Lys Gln 545 550 555 560 560 Leu Asp Leu Asp Leu Arg Gly Asn Leu Ala Asn Ile Ala Glu Ala Gly 565 570 575 Val Gly Phe Ala Cys Val Phe Thr Lys Val Phe Glu Glu Val Phe Ala 580 585 590 Val Lys Ala Asp Phe Ala Thr Gly Tyr Ser Met Gly Glu Val Ser Met 595 600 605 T yr Ala Ala Leu Gly Cys Trp Gln Gln Pro Gly Leu Met Ser Ala Arg 610 615 620 Leu Ala Gln Ser Asn Thr Phe Asn His Gln Leu Cys Gly Glu Le G Ar G 625 630 630 635 640 Thr Leu Arg Gln His Trp Gly Met Asp Asp Val Ala Asn Gly Thr Phe 645 650 655 Glu Gln Ile Trp Glu Thr Tyr Thr Ile Lys Ala Thr Ile Glu Gln Val 660 665 670 Glu Ile Ala Ser Ala Asp Glu Asp Arg Val Tyr Cys Thr Ile Ile Asn 675 680 685 Thr Pro Asp Ser Leu Leu Leu Ala Gly Tyr Pro Glu Ala Cys Gln Arg 690 695 700 Val Ile Lys Asn Leu Gly Val Arg Ala Met Ala Leu Asn Met Ala Asn 705 710 715 720 Ala Ile His Ser Ala Pro Ala Tyr Ala Glu Tyr Asp His Met Val Glu 725 730 735 Leu Tyr His Met Asp Val Thr Pro Arg Ile Asn Thr Lys Met Tyr Ser 740 745 750 Ser Ser Cys Tyr Leu Pro Ile Pro Gln Arg Ser Lys Ala Ile Ser His 755 760 765 Ser Ile Ala Lys Cys Leu Cys Asp Val Val Asp Phe Pro Arg Leu Val 770 775 780 Asn Thr Leu His Asp Lys Gly Ala Arg Val Phe Ile Glu Met Gly Pro 785 790 795 800 Gly Arg Ser Leu Cys Ser Trp Val Asp Lys Ile Leu Val Asn Asn Asp 805 810 815Gly Asp Asn Gln Lys Gln Ser Gln His Val Ser Val Pro Val Asn Ala 820 825 830 Lys Gly Thr Ser Asp Glu Leu Thr Tyr Ile Arg Ala Ile Ala Lys Leu 835 840 845 Ile Ser His Gly Val Asn Leu Asn Leu Asp Ser Leu Phe Asn Gly Ser 850 855 860 Ile Leu Val Lys Ala Gly Arg Ile Glu Asn Thr Asn Lys 865 870 875

【００４８】 <210> 6 <211> 6111 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 atggatttaa agagagtaat tatggaaaat attgcagtag taggtattgc taatttgttc 60 ccgggctcac aagcaccgga tcaattttgg cagcaattgc ttgaacaaca agattgccgc 120 agtaaggcga ccgctgttca aatgggcgtt gatcctgcta aatataccgc taacaaaggt 180 gatacagata agttttactg tgtgcacggc ggttacatca gtgatttcaa ttttgatgct 240 tcaggttatc aactcgataa tgattattta gccggtttag atgaccttaa tcagtggggg 300 ctttatgtaa cgaaacaagc gcttaccgat gcaggttatt ggggcagtac tgcattagaa 360 aactgtggtg tgattttagg taatttgtca ttcccaacca agtcatctaa tcagctgttt 420 atgcctttgt atcatcaagt tgttgataat gcattaaagt cggtattaca tcctgatttt 480 caactatcac attacacggc accgaaaaaa atacatgccg ataatgcatt agtagctggt 540 tatccagctg cattgatcgc gcaagctgcg ggtcttggtg ggtcgcactt tgcactggat 600 gcggcttgtg cttcatcttg ctatagcgtt aagttagcgt gtgattacct gcatacgggt 660 aaagccgaca tgatgcttgc tggtgcggta tctgcagcag atccaatgtt cgttaatatg 720 ggtttctcga tattccaagc ttatccagct aacaatgttc atgccccgtt tgaccaaaac 780 tcacaaggtc tatttgccgg tgaaggcgcg ggcatgatgg tattgaaacg tcaaagcgat 840 gcagtacgcg atggtgatca tatttacgcc attatcaaag gcggcgcatt atcgaatgac 900 ggtaaaggcg agtttgtatt aagcccgaac accaagggcc aagtattagt atatgaacgt 960 gcttatgccg atgctgatgt tgatccgagt accgttgatt atatcgaatg tcatgcaacg 1020 ggcacaccta aaggtgacaa tgttgaattg cgttcgatgg aaaccttttt cagtcgcgta 1080 aataacaaac cattactggg ttcggttaaa tctaaccttg gtcatttgtt aacagccgct 1140 ggtatgccgg gcatgacgaa agctatgtta gcgttaggta aaggtcttat tcctgcaacg 1200 attaatttaa agcaaccgct gcaatctaaa aacggttact ttactggcga gcaaatgcca 1260 acgacgactg tgccttggcc aacaacgccg ggtgccaaga cagataaacc gcgtaccgca 1320 ggtgtgagtg tatttggttt tggtggcagt aacgctcatt tggtattaca acagccaacg 1380 caaacactcg agactaattt tagtgttgct aaaccacgtg agtcattagc tattattggt 1440 atggacagcc attttggtag tgccagtaat ttagcgcagt tcaaaacctt attaaataat 1500 aatcagaata ccttccgtga attaccagaa caacgctgga aaggcatgga aagtaacgct 1560 aatgtcatgc agtcgttaca attacgcaaa gcgcctaagg gtagttacgt tgaacagctt 1620 gatattgatt tcttgcgttt caaagtaccg cctaatgaaa aagattgctt gatcccgcaa 1680 caactaatga tgatgcaagt ggcagacaat gctgcgaaag acgcaggttt agtagaaggt 1740 cgtaatgttg ctgtattagt ggctatgggc atggaactgg aattacatca gtatcgtggt 1800 cgcgttaatc taacgacgca aattgaagac agcttattac agcagggtat taacctgacg 1860 gctgagcaac gtgaagaact aaccaatatt gctaaagacg gtgtcgcttc ggctgcccag 1920 ctaaatcagt atactagctt cattggtaat atcatggcgt cacgtatttc ggcgttatgg 1980 gatttctctg gtccagctat tactgtttct gctgaagaaa actcagttta tcgttgtgtt 2040 gaattagctg aaaacctatt tcaaaccagt gatgttgaag ccgttattat tgctgctgtt 2100 gatttgtcag gttcaattga aaacattacc ttacgtcagc actacggtcc agttaatgaa 2160 aagggtcctg taagtgaatg tggtcccgta agtgaaaatg gctcggtaaa cggaagtggc 2220 tcagtaacca acaatattct tgatcagcaa caatggctgg tgggtgaagg cgcagcggct 2280 attgtcgtta aaccgtcatc acaagtaact gccgagcaag tctatgcgcg tattgatgca 2340 gttagttttg cccctggtag tgatgctaaa gcaattacga ttgcggcgga caaagcatta 2400 acattagccg gtatcagtgc ggcggatgta gcgagtgttg aagcacatgc aagtggtttt 2460 agtgccgaaa ataatgctga aaaaaccgcg ttaccgactt tatacccaag tgcaaacatc 2520 agttcggtga aagccaatat tggtcatacg tttaatgcat cgggtatggc gagtattatt 2580 aaaactgcct tgctgttaga tcagaacacg agtcaagatc agaaaagcca acatattgct 2640 attaacggcc taggtcgtga taacagctgt gcgcatctta tcttatcgag ttctgaacaa 2700 gcacatcaag ctgcaccagc gcctgtatct ggtatggcca agcaacgccc acaattagtt 2760 aaaaccatca aactaggtgg tcaactaatt agcaatgcga ttgttaacag tgcgagttca 2820 tctttggatg cgattaaagc gcagtttgct ggtaagcatt taaacaaagt taaccagcca 2880 gtaatgatgg ataacctgaa accacaaggc attcgcgctc attcaatgaa tgaacatgtg 2940 gtgacgagac cttctagtac tcaagtgtct catacccaag cgttcactca agcgtctatt 3000 caagcatcta ttcaagaaca agagctagca caagagcaag tacaaaatat gcaagctaca 3060 gcagccgctg taagttcacc cctttctcaa catcaacata caacgcagcc cgtagcggca 3120 ccgagcgttg ttggagtgac tgtgaaacat aaagcaagta accaaattca tcagcaagcg 3180 tctacgcata aagcattttt agaaagtcgt ttagctgcac agaaaaacct atcgcaactt 3240 gttgaactgc aaaccaagct atcaacacaa actgctagtg acaatacgtc taacaataca 3300 tctagcaata cagtgttaac aaatcctgta tcagcaactg caacgccatt aacactcgta 3360 tctactcttg ttacaaataa gcccgtagtc gtgacaagcc taaccagcac tgaagcaaaa 3420 gcgcaagcag cagctacaca agctggtttt cagataaaag gacctgttgg ttacaactat 3480 ccaccactgc agttaattga gcgttataac aaacctgaaa aagtgattta cgatcaagct 3540 gatttggttg aattcgctga aggtgatatt ggtaaggtat ttggtgctga atacaatatt 3600 attgatggct actcgcgtcg tgtacgtcta ccaacctcag attatctatt agtaacacgt 3660 gttactgaac ttgatgccaa ggtgcatgaa tacaagaaat catacatgtg cactgaatat 3720 gatgtacctg ttgatgcacc gttcttaatc gatggtcaga tcccttggtc tgttgcagta 3780 gaatcaggcc agtgtgattt aatgttgatt tcatacatcg gtattgattt ccaagctaaa 3840 ggcgagcgtg tttaccgttt acttgattgt gaattaacgt tccttgaaga gatggctttc 3900 ggtggcgata ctttacgtta cgagatccac attgattcgt atgcacgtaa cggcgagcaa 3960 ttattattct tcttccatta cgattgttac gtaggggata agaaggtact tatcatgcgt 4020 aatggttgtg ctggtttctt tactgacgaa gaactttctg acggtaaagg cgttattcat 4080 aacgacaaag acaaagctga atttagcaat gctgttaaat catcattcac gccgttatta 4140 caacataacc gtggtcaata cgattataac gacatgatga agctggttaa tggcgacgtg 4200 gcgagttgtt ttggtccgca atatgatcaa ggtggccgta atccatcgtt gaaattctcg 4260 tctgagaagt tcttgatgat tgaacgtatt accaagatag atccaaccgg tggtcattgg 4320 ggacttggcc tgttagaagg tcagaaagat ttagaccctg agcattggta tttcccttgt 4380 cactttaaag gtgatcaagt aatggctggt tcgttgatgt cggaaggttg tggccaaatg 4440 gcgatgttct tcatgctgtc tcttggtatg catactaatg tgaacaacgc tcgtttccaa 4500 ccgctaccag gtgaatcaca aacggtacgt tgtcgtgggc aagtactgcc acagcgcaat 4560 accttaactt accgtatgga agtaacggcg atgggtatgc atccacagcc attcatgaaa 4620 gctaatattg atattttgct tgatggtaaa gtggttgttg atttcaaaaa cttgagcgtg 4680 atgattagcg aacaagatga acattcagat taccctgtaa cactgccgag taatgtgcag 4740 cttaaaacaa tttcgtctgc tgctgtaacg cctgttgctg taactacgac gcctgttgct 4800 ttagctacaa cgaacaatgc cgatctggat gaacgtggtg ttgaaccgtt taagtatcct 4860 gaacgtccgt taatgcgcgt agagtcagac ttgtctgcac cgaaaagcaa aggtgtgaca 4920 ccgattaagc attttgaagc gcctgttgtt gctggccacc atagagtgcc taaccaagca 4980 ccgtttacac cttggcatat gtttgagttt gcgacgggta atatctctaa ctgttttggt 5040 cctgactttg atgtttatga aggtcgtatt ccaccacgta cgccttgtgg cgatttacaa 5100 gttgttactc aggttgtgga agtgcagggc gaacgtcttg atcttaaaaa tccatcaagc 5160 tgtatagctg aatactatgt tcctgaagat gcttggtact ttactaaaaa cagccatgaa 5220 aactggatgc catattcatt aatcatggaa attgcattgc aaccaaatgg ctttatttct 5280 ggttacatgg gcacgacgct taaatatcct gaaaaagatc tgttcttccg taaccttgat 5340 ggtagcggca cgttattaaa gcagattgat ttacgcggca aaacgattgt gaataaatca 5400 gtattagtta gtacggctat tgctggtggc gcgattattc aaagcttcac gtttgatatg 5460 tctgtagatg gcgagctatt ttatactggt aaagcggtat ttggttactt tagtggtgaa 5520 tcgctgacta accaactggg cattgataac ggtaagacga ctaatgcttg gtttgttgat 5580 aacaatacgc ctgcagctaa tattgatgtg ttcgatttaa cggatcagtc tttagcgtta 5640 tacaaagcgc ctgtaggtaa gccgcattat aaattggctg gtggccagat gaacttcatc 5700 gatactgtgt ctgttgttga aggcggcggt aaagcggatg tggcttatgt ttatggcgaa 5760 cgtacgattg atgctgatga ttggttcttc cgttatcact tccaccaaga tccggtgatg 5820 cctggttcgt taggtgttga agctattatt gagctgatgc aaacctatgc gcttaaaaat 5880 gacttgggtg cacagtttac tagtcctcgt tttgttgcgc cgatgacgaa agttgattgg 5940 aaatatcgtg gtcaaattac gccgttgaat aagcagatgt ctctggacgt gcatattacc 6000 gagatcatta ctgaatcagg tgaagtaaga ctcgttggtg atgcgaacct gtctaaagat 6060 ggtctgcgta tttatgaagt gaagaatatt gttttaagta ttgttgaagc g 6111<210> 6 <211> 6111 <212> DNA <213> Shewanella sp. SCRC-21406 (FERM BP-5979) <400> 1 atggatttaa agagagtaat tatggaaaat attgcagtag taggtattgc taatttgttc 60 ccgggctcac aagcaccggagcatca tcaattg gagcagca tcaattg gagcagca tcaattg gagcagca tcaattg aatgggcgtt gatcctgcta aatataccgc taacaaaggt 180 gatacagata agttttactg tgtgcacggc ggttacatca gtgatttcaa ttttgatgct 240 tcaggttatc aactcgataa tgattattta gccggtttag atgaccttaa tcagtggggg 300 ctttatgtaa cgaaacaagc gcttaccgat gcaggttatt ggggcagtac tgcattagaa 360 aactgtggtg tgattttagg taatttgtca ttcccaacca agtcatctaa tcagctgttt 420 atgcctttgt atcatcaagt tgttgataat gcattaaagt cggtattaca tcctgatttt 480 caactatcac attacacggc accgaaaaaa atacatgccg ataatgcatt agtagctggt 540 tatccagctg cattgatcgc gcaagctgcg ggtcttggtg ggtcgcactt tgcactggat 600 gcggcttgtg cttcatcttg ctatagcgtt aagttagcgt gtgattacct gcatacgggt 660 aaagccgaca tgatgcttgc tggtcggta tctgcagcag atcctcgatcgtcgtcgtcgtcgtcgcgtcgattcgtcgcgtcgcgtcgatcgtcgcgtcgcgtcgatcgtcgatcgtcgatcgtcgatcgtcgatcgtcgatcgtcgatcgtcgatgtcgtcgtcgtcgtcgtcgatgtcgtcgtcgtcgtcgtcgtgtcgtcgtcgtcgtcgtcgtcgttgcgtcgtcgtcgtcgtcgctgtcgtcgctgtcgctgtcgtcgtgtgtgcgtcgttggtgtcgtagtgtcgcgtcgtcgtcgtcgtcgttgcgttgcgtgg caaaac 780 tcacaaggtc tatttgccgg tgaaggcgcg ggcatgatgg tattgaaacg tcaaagcgat 840 gcagtacgcg atggtgatca tatttacgcc attatcaaag gcggcgcatt atcgaatgac 900 ggtaaaggcg agtttgtatt aagcccgaac accaagggcc aagtattagt atatgaacgt 960 gcttatgccg atgctgatgt tgatccgagt accgttgatt atatcgaatg tcatgcaacg 1020 ggcacaccta aaggtgacaa tgttgaattg cgttcgatgg aaaccttttt cagtcgcgta 1080 aataacaaac cattactggg ttcggttaaa tctaaccttg gtcatttgtt aacagccgct 1140 ggtatgccgg gcatgacgaa agctatgtta gcgttaggta aaggtcttat tcctgcaacg 1200 attaatttaa agcaaccgct gcaatctaaa aacggttact ttactggcga gcaaatgcca 1260 acgacgactg tgccttggcc aacaacgccg ggtgccaaga cagataaacc gcgtaccgca 1320 ggtgtgagtg tatttggttt tggtggcagt aacgctcatt tggtattaca acagccaacg 1380 caaacactcg agactaattt tagtgttgct aaaccacgtg agtcattagc tattattggt 1440 atggacagcc attttggtag tgccagtaat ttagcgcagt tcaaaacctt attaaataat 1500 aatcagaata ccttccgtga attaccagaa caacgctgga aaggcatgga aagtaacgct 1560 aatgtcatgc agtcgttaca attacgcaaa gcgcctaagg gtagttacgt tgaacagctt 162 0 gatattgatt tcttgcgttt caaagtaccg cctaatgaaa aagattgctt gatcccgcaa 1680 caactaatga tgatgcaagt ggcagacaat gctgcgaaag acgcaggttt agtagaaggt 1740 cgtaatgttg ctgtattagt ggctatgggc atggaactgg aattacatca gtatcgtggt 1800 cgcgttaatc taacgacgca aattgaagac agcttattac agcagggtat taacctgacg 1860 gctgagcaac gtgaagaact aaccaatatt gctaaagacg gtgtcgcttc ggctgcccag 1920 ctaaatcagt atactagctt cattggtaat atcatggcgt cacgtatttc ggcgttatgg 1980 gatttctctg gtccagctat tactgtttct gctgaagaaa actcagttta tcgttgtgtt 2040 gaattagctg aaaacctatt tcaaaccagt gatgttgaag ccgttattat tgctgctgtt 2100 gatttgtcag gttcaattga aaacattacc ttacgtcagc actacggtcc agttaatgaa 2160 aagggtcctg taagtgaatg tggtcccgta agtgaaaatg gctcggtaaa cggaagtggc 2220 tcagtaacca acaatattct tgatcagcaa caatggctgg tgggtgaagg cgcagcggct 2280 attgtcgtta aaccgtcatc acaagtaact gccgagcaag tctatgcgcg tattgatgca 2340 gttagttttg cccctggtag tgatgctaaa gcaattacga ttgcggcgga caaagcatta 2400 acattagccg gtatcagtgc ggcggatgta gcgagtgttg aagcacatgc aagtggtttt 2460 agtg ccgaaa ataatgctga aaaaaccgcg ttaccgactt tatacccaag tgcaaacatc 2520 agttcggtga aagccaatat tggtcatacg tttaatgcat cgggtatggc gagtattatt 2580 aaaactgcct tgctgttaga tcagaacacg agtcaagatc agaaaagcca acatattgct 2640 attaacggcc taggtcgtga taacagctgt gcgcatctta tcttatcgag ttctgaacaa 2700 gcacatcaag ctgcaccagc gcctgtatct ggtatggcca agcaacgccc acaattagtt 2760 aaaaccatca aactaggtgg tcaactaatt agcaatgcga ttgttaacag tgcgagttca 2820 tctttggatg cgattaaagc gcagtttgct ggtaagcatt taaacaaagt taaccagcca 2880 gtaatgatgg ataacctgaa accacaaggc attcgcgctc attcaatgaa tgaacatgtg 2940 gtgacgagac cttctagtac tcaagtgtct catacccaag cgttcactca agcgtctatt 3000 caagcatcta ttcaagaaca agagctagca caagagcaag tacaaaatat gcaagctaca 3060 gcagccgctg taagttcacc cctttctcaa catcaacata caacgcagcc cgtagcggca 3120 ccgagcgttg ttggagtgac tgtgaaacat aaagcaagta accaaattca tcagcaagcg 3180 tctacgcata aagcattttt agaaagtcgt ttagctgcac agaaaaacct atcgcaactt 3240 gttgaactgc aaaccaagct atcaacacaa actgctagtg acaatacgtc taacaataca 3300 tctagcaata cagtgttaac aaatcctgta tcagcaactg caacgccatt aacactcgta 3360 tctactcttg ttacaaataa gcccgtagtc gtgacaagcc taaccagcac tgaagcaaaa 3420 gcgcaagcag cagctacaca agctggtttt cagataaaag gacctgttgg ttacaactat 3480 ccaccactgc agttaattga gcgttataac aaacctgaaa aagtgattta cgatcaagct 3540 gatttggttg aattcgctga aggtgatatt ggtaaggtat ttggtgctga atacaatatt 3600 attgatggct actcgcgtcg tgtacgtcta ccaacctcag attatctatt agtaacacgt 3660 gttactgaac ttgatgccaa ggtgcatgaa tacaagaaat catacatgtg cactgaatat 3720 gatgtacctg ttgatgcacc gttcttaatc gatggtcaga tcccttggtc tgttgcagta 3780 gaatcaggcc agtgtgattt aatgttgatt tcatacatcg gtattgattt ccaagctaaa 3840 ggcgagcgtg tttaccgttt acttgattgt gaattaacgt tccttgaaga gatggctttc 3900 ggtggcgata ctttacgtta cgagatccac attgattcgt atgcacgtaa cggcgagcaa 3960 ttattattct tcttccatta cgattgttac gtaggggata agaaggtact tatcatgcgt 4020 aatggttgtg ctggtttctt tactgacgaa gaactttctg acggtaaagg cgttattcat 4080 aacgacaaag acaaagctga atttagcaat gctgttaaat catcattcac gccgttatta 4140 caacataacc gtggt caata cgattataac gacatgatga agctggttaa tggcgacgtg 4200 gcgagttgtt ttggtccgca atatgatcaa ggtggccgta atccatcgtt gaaattctcg 4260 tctgagaagt tcttgatgat tgaacgtatt accaagatag atccaaccgg tggtcattgg 4320 ggacttggcc tgttagaagg tcagaaagat ttagaccctg agcattggta tttcccttgt 4380 cactttaaag gtgatcaagt aatggctggt tcgttgatgt cggaaggttg tggccaaatg 4440 gcgatgttct tcatgctgtc tcttggtatg catactaatg tgaacaacgc tcgtttccaa 4500 ccgctaccag gtgaatcaca aacggtacgt tgtcgtgggc aagtactgcc acagcgcaat 4560 accttaactt accgtatgga agtaacggcg atgggtatgc atccacagcc attcatgaaa 4620 gctaatattg atattttgct tgatggtaaa gtggttgttg atttcaaaaa cttgagcgtg 4680 atgattagcg aacaagatga acattcagat taccctgtaa cactgccgag taatgtgcag 4740 cttaaaacaa tttcgtctgc tgctgtaacg cctgttgctg taactacgac gcctgttgct 4800 ttagctacaa cgaacaatgc cgatctggat gaacgtggtg ttgaaccgtt taagtatcct 4860 gaacgtccgt taatgcgcgt agagtcagac ttgtctgcac cgaaaagcaa aggtgtgaca 4920 ccgattaagc attttgaagc gcctgttgtt gctggccacc atagagtgcc taaccaagca 4980 ccgtttacac cttggcatat gtttgagttt gcgacgggta atatctctaa ctgttttggt 5040 cctgactttg atgtttatga aggtcgtatt ccaccacgta cgccttgtgg cgatttacaa 5100 gttgttactc aggttgtgga agtgcagggc gaacgtcttg atcttaaaaa tccatcaagc 5160 tgtatagctg aatactatgt tcctgaagat gcttggtact ttactaaaaa cagccatgaa 5220 aactggatgc catattcatt aatcatggaa attgcattgc aaccaaatgg ctttatttct 5280 ggttacatgg gcacgacgct taaatatcct gaaaaagatc tgttcttccg taaccttgat 5340 ggtagcggca cgttattaaa gcagattgat ttacgcggca aaacgattgt gaataaatca 5400 gtattagtta gtacggctat tgctggtggc gcgattattc aaagcttcac gtttgatatg 5460 tctgtagatg gcgagctatt ttatactggt aaagcggtat ttggttactt tagtggtgaa 5520 tcgctgacta accaactggg cattgataac ggtaagacga ctaatgcttg gtttgttgat 5580 aacaatacgc ctgcagctaa tattgatgtg ttcgatttaa cggatcagtc tttagcgtta 5640 tacaaagcgc ctgtaggtaa gccgcattat aaattggctg gtggccagat gaacttcatc 5700 gatactgtgt ctgttgttga aggcggcggt aaagcggatg tggcttatgt ttatggcgaa 5760 cgtacgattg atgctgatga ttggttcttc cgttatcact tccaccaaga tccggtgatg 5820 cctggttcgt taggtgttga agctat tatt gagctgatgc aaacctatgc gcttaaaaat 5880 gacttgggtg cacagtttac tagtcctcgt tttgttgcgc cgatgacgaa agttgattgg 5940 aaatatcgtg gtcaaattac gccgttgaat aagcagatgt ctctggacgt gcatattacc 6000 gagatcatta ctgaatcagg tgaagtaaga ctcgttggtg atgcgaacct gtctaaagat 6060 ggtctgcgta tttatgaagt gaagaatatt gttttaagta ttgttgaagc g 6111

【００４９】 <210> 7 <211> 2037 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 Met Asp Leu Lys Arg Val Ile Met Glu Asn Ile Ala Val Val Gly Ile 1 5 10 15 Ala Asn Leu Phe Pro Gly Ser Gln Ala Pro Asp Gln Phe Trp Gln Gln 20 25 30 Leu Leu Glu Gln Gln Asp Cys Arg Ser Lys Ala Thr Ala Val Gln Met 35 40 45 Gly Val Asp Pro Ala Lys Tyr Thr Ala Asn Lys Gly Asp Thr Asp Lys 50 55 60 Phe Tyr Cys Val His Gly Gly Tyr Ile Ser Asp Phe Asn Phe Asp Ala 65 70 75 80 Ser Gly Tyr Gln Leu Asp Asn Asp Tyr Leu Ala Gly Leu Asp Asp Leu 85 90 95 Asn Gln Trp Gly Leu Tyr Val Thr Lys Gln Ala Leu Thr Asp Ala Gly 100 105 110 Tyr Trp Gly Ser Thr Ala Leu Glu Asn Cys Gly Val Ile Leu Gly Asn 115 120 125 Leu Ser Phe Pro Thr Lys Ser Ser Asn Gln Leu Phe Met Pro Leu Tyr 130 135 140 His Gln Val Val Asp Asn Ala Leu Lys Ser Val Leu His Pro Asp Phe 145 150 155 160 Gln Leu Ser His Tyr Thr Ala Pro Lys Lys Ile His Ala Asp Asn Ala 165 170 175 Leu Val Ala Gly Tyr Pro Ala Ala Leu Ile Ala Gln Ala Ala Gly Leu 180 185 190 Gly Gly Ser His Phe Ala Leu Asp Ala Ala Cys Ala Ser Ser Cys Tyr 195 200 205 Ser Val Lys Leu Ala Cys Asp Tyr Leu His Thr Gly Lys Ala Asp Met 210 215 220 Met Leu Ala Gly Ala Val Ser Ala Ala Asp Pro Met Phe Val Asn Met 225 230 235 240 Gly Phe Ser Ile Phe Gln Ala Tyr Pro Ala Asn Asn Val His Ala Pro 245 250 255 Phe Asp Gln Asn Ser Gln Gly Leu Phe Ala Gly Glu Gly Ala Gly Met 260 265 270 Met Val Leu Lys Arg Gln Ser Asp Ala Val Arg Asp Gly Asp His Ile 275 280 285 Tyr Ala Ile Ile Lys Gly Gly Ala Leu Ser Asn Asp Gly Lys Gly Glu 290 295 300 Phe Val Leu Ser Pro Asn Thr Lys Gly Gln Val Leu Val Tyr Glu Arg 305 310 315 320 Ala Tyr Ala Asp Ala Asp Val Asp Pro Ser Thr Val Asp Tyr Ile Glu 325 330 335 Cys His Ala Thr Gly Thr Pro Lys Gly Asp Asn Val Glu Leu Arg Ser 340 345 350 Met Glu Thr Phe Phe Ser Arg Val Asn Asn Lys Pro Leu Leu Gly Ser 355 360 365 Val Lys Ser Asn Leu Gly His Leu Leu Thr Ala Ala Gly Met Pro Gly 370 375 380 Met Thr Lys Ala Met Leu Ala Leu Gly Lys Gly Leu Ile Pro Ala Thr 385 390 395 400 Ile Asn Leu Lys Gln Pro Leu Gln Ser Lys Asn Gly Tyr Phe Thr Gly 405 410 415 Glu Gln Met Pro Thr Thr Thr Val Pro Trp Pro Thr Thr Pro Gly Ala 420 425 430 Lys Thr Asp Lys Pro Arg Thr Ala Gly Val Ser Val Phe Gly Phe Gly 435 440 445 Gly Ser Asn Ala His Leu Val Leu Gln Gln Pro Thr Gln Thr Leu Glu 450 455 460 Thr Asn Phe Ser Val Ala Lys Pro Arg Glu Ser Leu Ala Ile Ile Gly 465 470 475 480 Met Asp Ser His Phe Gly Ser Ala Ser Asn Leu Ala Gln Phe Lys Thr 485 490 495 Leu Leu Asn Asn Asn Gln Asn Thr Phe Arg Glu Leu Pro Glu Gln Arg 500 505 510 Trp Lys Gly Met Glu Ser Asn Ala Asn Val Met Gln Ser Leu Gln Leu 515 520 525 Arg Lys Ala Pro Lys Gly Ser Tyr Val Glu Gln Leu Asp Ile Asp Phe 530 535 540 Leu Arg Phe Lys Val Pro Pro Asn Glu Lys Asp Cys Leu Ile Pro Gln 545 550 555 560 Gln Leu Met Met Met Gln Val Ala Asp Asn Ala Ala Lys Asp Ala Gly 565 570 575 Leu Val Glu Gly Arg Asn Val Ala Val Leu Val Ala Met Gly Met Glu 580 585 590 Leu Glu Leu His Gln Tyr Arg Gly Arg Val Asn Leu Thr Thr Gln Ile 595 600 605 Glu Asp Ser Leu Leu Gln Gln Gly Ile Asn Leu Thr Ala Glu Gln Arg 610 615 620 Glu Glu Leu Thr Asn Ile Ala Lys Asp Gly Val Ala Ser Ala Ala Gln 625 630 635 640 Leu Asn Gln Tyr Thr Ser Phe Ile Gly Asn Ile Met Ala Ser Arg Ile 645 650 655 Ser Ala Leu Trp Asp Phe Ser Gly Pro Ala Ile Thr Val Ser Ala Glu 660 665 670 Glu Asn Ser Val Tyr Arg Cys Val Glu Leu Ala Glu Asn Leu Phe Gln 675 680 685 Thr Ser Asp Val Glu Ala Val Ile Ile Ala Ala Val Asp Leu Ser Gly 690 695 700 Ser Ile Glu Asn Ile Thr Leu Arg Gln His Tyr Gly Pro Val Asn Glu 705 710 715 720 Lys Gly Pro Val Ser Glu Cys Gly Pro Val Ser Glu Asn Gly Ser Val 725 730 735 Asn Gly Ser Gly Ser Val Thr Asn Asn Ile Leu Asp Gln Gln Gln Trp 740 745 750 Leu Val Gly Glu Gly Ala Ala Ala Ile Val Val Lys Pro Ser Ser Gln 755 760 765 Val Thr Ala Glu Gln Val Tyr Ala Arg Ile Asp Ala Val Ser Phe Ala 770 775 780 Pro Gly Ser Asp Ala Lys Ala Ile Thr Ile Ala Ala Asp Lys Ala Leu 785 790 795 800 Thr Leu Ala Gly Ile Ser Ala Ala Asp Val Ala Ser Val Glu Ala His 805 810 815 Ala Ser Gly Phe Ser Ala Glu Asn Asn Ala Glu Lys Thr Ala Leu Pro 820 825 830 Thr Leu Tyr Pro Ser Ala Asn Ile Ser Ser Val Lys Ala Asn Ile Gly 835 840 845 His Thr Phe Asn Ala Ser Gly Met Ala Ser Ile Ile Lys Thr Ala Leu 850 855 860 Leu Leu Asp Gln Asn Thr Ser Gln Asp Gln Lys Ser Gln His Ile Ala 865 870 875 880 Ile Asn Gly Leu Gly Arg Asp Asn Ser Cys Ala His Leu Ile Leu Ser 885 890 895 Ser Ser Glu Gln Ala His Gln Ala Ala Pro Ala Pro Val Ser Gly Met 900 905 910 Ala Lys Gln Arg Pro Gln Leu Val Lys Thr Ile Lys Leu Gly Gly Gln 915 920 925 Leu Ile Ser Asn Ala Ile Val Asn Ser Ala Ser Ser Ser Leu Asp Ala 930 935 940 Ile Lys Ala Gln Phe Ala Gly Lys His Leu Asn Lys Val Asn Gln Pro 945 950 955 960 Val Met Met Asp Asn Leu Lys Pro Gln Gly Ile Arg Ala His Ser Met 965 970 975 Asn Glu His Val Val Thr Arg Pro Ser Ser Thr Gln Val Ser His Thr 980 985 990 Gln Ala Phe Thr Gln Ala Ser Ile Gln Ala Ser Ile Gln Glu Gln Glu 995 1000 1005 Leu Ala Gln Glu Gln Val Gln Asn Met Gln Ala Thr Ala Ala Ala Val 1010 1015 1020 Ser Ser Pro Leu Ser Gln His Gln His Thr Thr Gln Pro Val Ala Ala 1025 1030 1035 1040 Pro Ser Val Val Gly Val Thr Val Lys His Lys Ala Ser Asn Gln Ile 1045 1050 1055 His Gln Gln Ala Ser Thr His Lys Ala Phe Leu Glu Ser Arg Leu Ala 1060 1065 1070 Ala Gln Lys Asn Leu Ser Gln Leu Val Glu Leu Gln Thr Lys Leu Ser 1075 1080 1085 Thr Gln Thr Ala Ser Asp Asn Thr Ser Asn Asn Thr Ser Ser Asn Thr 1090 1095 1100 Val Leu Thr Asn Pro Val Ser Ala Thr Ala Thr Pro Leu Thr Leu Val 1105 1110 1115 1120 Ser Thr Leu Val Thr Asn Lys Pro Val Val Val Thr Ser Leu Thr Ser 1125 1130 1135 Thr Glu Ala Lys Ala Gln Ala Ala Ala Thr Gln Ala Gly Phe Gln Ile 1140 1145 1150 Lys Gly Pro Val Gly Tyr Asn Tyr Pro Pro Leu Gln Leu Ile Glu Arg 1155 1160 1165 Tyr Asn Lys Pro Glu Lys Val Ile Tyr Asp Gln Ala Asp Leu Val Glu 1170 1175 1180 Phe Ala Glu Gly Asp Ile Gly Lys Val Phe Gly Ala Glu Tyr Asn Ile 1185 1190 1195 1200 Ile Asp Gly Tyr Ser Arg Arg Val Arg Leu Pro Thr Ser Asp Tyr Leu 1205 1210 1215 Leu Val Thr Arg Val Thr Glu Leu Asp Ala Lys Val His Glu Tyr Lys 1220 1225 1230 Lys Ser Tyr Met Cys Thr Glu Tyr Asp Val Pro Val Asp Ala Pro Phe 1235 1240 1245 Leu Ile Asp Gly Gln Ile Pro Trp Ser Val Ala Val Glu Ser Gly Gln 1250 1255 1260 Cys Asp Leu Met Leu Ile Ser Tyr Ile Gly Ile Asp Phe Gln Ala Lys 1265 1270 1275 1280 Gly Glu Arg Val Tyr Arg Leu Leu Asp Cys Glu Leu Thr Phe Leu Glu 1285 1290 1295 Glu Met Ala Phe Gly Gly Asp Thr Leu Arg Tyr Glu Ile His Ile Asp 1300 1305 1310 Ser Tyr Ala Arg Asn Gly Glu Gln Leu Leu Phe Phe Phe His Tyr Asp 1315 1320 1325 Cys Tyr Val Gly Asp Lys Lys Val Leu Ile Met Arg Asn Gly Cys Ala 1330 1335 1340 Gly Phe Phe Thr Asp Glu Glu Leu Ser Asp Gly Lys Gly Val Ile His 1345 1350 1355 1360 Asn Asp Lys Asp Lys Ala Glu Phe Ser Asn Ala Val Lys Ser Ser Phe 1365 1370 1375 Thr Pro Leu Leu Gln His Asn Arg Gly Gln Tyr Asp Tyr Asn Asp Met 1380 1385 1390 Met Lys Leu Val Asn Gly Asp Val Ala Ser Cys Phe Gly Pro Gln Tyr 1395 1400 1405 Asp Gln Gly Gly Arg Asn Pro Ser Leu Lys Phe Ser Ser Glu Lys Phe 1410 1415 1420 Leu Met Ile Glu Arg Ile Thr Lys Ile Asp Pro Thr Gly Gly His Trp 1425 1430 1435 1440 Gly Leu Gly Leu Leu Glu Gly Gln Lys Asp Leu Asp Pro Glu His Trp 1445 1450 1455 Tyr Phe Pro Cys His Phe Lys Gly Asp Gln Val Met Ala Gly Ser Leu 1460 1465 1470 Met Ser Glu Gly Cys Gly Gln Met Ala Met Phe Phe Met Leu Ser Leu 1475 1480 1485 Gly Met His Thr Asn Val Asn Asn Ala Arg Phe Gln Pro Leu Pro Gly 1490 1495 1500 Glu Ser Gln Thr Val Arg Cys Arg Gly Gln Val Leu Pro Gln Arg Asn 1505 1510 1515 1520 Thr Leu Thr Tyr Arg Met Glu Val Thr Ala Met Gly Met His Pro Gln 1525 1530 1535 Pro Phe Met Lys Ala Asn Ile Asp Ile Leu Leu Asp Gly Lys Val Val 1540 1545 1550 Val Asp Phe Lys Asn Leu Ser Val Met Ile Ser Glu Gln Asp Glu His 1555 1560 1565 Ser Asp Tyr Pro Val Thr Leu Pro Ser Asn Val Gln Leu Lys Thr Ile 1570 1575 1580 Ser Ser Ala Ala Val Thr Pro Val Ala Val Thr Thr Thr Pro Val Ala 1585 1590 1595 1600 Leu Ala Thr Thr Asn Asn Ala Asp Leu Asp Glu Arg Gly Val Glu Pro 1605 1610 1615 Phe Lys Tyr Pro Glu Arg Pro Leu Met Arg Val Glu Ser Asp Leu Ser 1620 1625 1630 Ala Pro Lys Ser Lys Gly Val Thr Pro Ile Lys His Phe Glu Ala Pro 1635 1640 1645 Val Val Ala Gly His His Arg Val Pro Asn Gln Ala Pro Phe Thr Pro 1650 1655 1660 Trp His Met Phe Glu Phe Ala Thr Gly Asn Ile Ser Asn Cys Phe Gly 1665 1670 1675 1680 Pro Asp Phe Asp Val Tyr Glu Gly Arg Ile Pro Pro Arg Thr Pro Cys 1685 1690 1695 Gly Asp Leu Gln Val Val Thr Gln Val Val Glu Val Gln Gly Glu Arg 1700 1705 1710 Leu Asp Leu Lys Asn Pro Ser Ser Cys Ile Ala Glu Tyr Tyr Val Pro 1715 1720 1725 Glu Asp Ala Trp Tyr Phe Thr Lys Asn Ser His Glu Asn Trp Met Pro 1730 1735 1740 Tyr Ser Leu Ile Met Glu Ile Ala Leu Gln Pro Asn Gly Phe Ile Ser 1745 1750 1755 1760 Gly Tyr Met Gly Thr Thr Leu Lys Tyr Pro Glu Lys Asp Leu Phe Phe 1765 1770 1775 Arg Asn Leu Asp Gly Ser Gly Thr Leu Leu Lys Gln Ile Asp Leu Arg 1780 1785 1790 Gly Lys Thr Ile Val Asn Lys Ser Val Leu Val Ser Thr Ala Ile Ala 1795 1800 1805 Gly Gly Ala Ile Ile Gln Ser Phe Thr Phe Asp Met Ser Val Asp Gly 1810 1815 1820 Glu Leu Phe Tyr Thr Gly Lys Ala Val Phe Gly Tyr Phe Ser Gly Glu 1825 1830 1835 1840 Ser Leu Thr Asn Gln Leu Gly Ile Asp Asn Gly Lys Thr Thr Asn Ala 1845 1850 1855 Trp Phe Val Asp Asn Asn Thr Pro Ala Ala Asn Ile Asp Val Phe Asp 1860 1865 1870 Leu Thr Asp Gln Ser Leu Ala Leu Tyr Lys Ala Pro Val Gly Lys Pro 1875 1880 1885 His Tyr Lys Leu Ala Gly Gly Gln Met Asn Phe Ile Asp Thr Val Ser 1890 1895 1900 Val Val Glu Gly Gly Gly Lys Ala Asp Val Ala Tyr Val Tyr Gly Glu 1905 1910 1915 1920 Arg Thr Ile Asp Ala Asp Asp Trp Phe Phe Arg Tyr His Phe His Gln 1925 1930 1935 Asp Pro Val Met Pro Gly Ser Leu Gly Val Glu Ala Ile Ile Glu Leu 1940 1945 1950 Met Gln Thr Tyr Ala Leu Lys Asn Asp Leu Gly Ala Gln Phe Thr Ser 1955 1960 1965 Pro Arg Phe Val Ala Pro Met Thr Lys Val Asp Trp Lys Tyr Arg Gly 1970 1975 1980 Gln Ile Thr Pro Leu Asn Lys Gln Met Ser Leu Asp Val His Ile Thr 1985 1990 1995 2000 Glu Ile Ile Thr Glu Ser Gly Glu Val Arg Leu Val Gly Asp Ala Asn 2005 2010 2015 Leu Ser Lys Asp Gly Leu Arg Ile Tyr Glu Val Lys Asn Ile Val Leu 2020 2025 2030 Ser Ile Val Glu Ala 2035 <210> 7 <211> 2037 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 Met Asp Leu Lys Arg Val Ile Met Glu Asn Ile Ala Val Val Gly Ile 1 5 10 15 Ala Asn Leu Phe Pro Gly Ser Gln Ala Pro Asp Gln Phe Trp Gln Gln 20 25 30 Leu Leu Glu Gln Gln Asp Cys Arg Ser Lys Ala Thr Ala Val Gln Met 35 40 45 Gly Val Asp Pro Ala Lys Tyr Thr Ala Asn Lys Gly Asp Thr Asp Lys 50 55 60 Phe Tyr Cys Val His Gly Gly Tyr Ile Ser Asp Phe Asn Phe Asp Ala 65 70 75 80 Ser Gly Tyr Gln Leu Asp Asn Asp Tyr Leu Ala Gly Leu Asp Asp Leu 85 90 95 Asn Gln Trp Gly Leu Tyr Val Thr Lys Gln Ala Leu Thr Asp Ala Gly 100 105 110 Tyr Trp Gly Ser Thr Ala Leu Glu Asn Cys Gly Val Ile Leu Gly Asn 115 120 125 Leu Ser Phe Pro Thr Lys Ser Ser Asn Gln Leu Phe Met Pro Leu Tyr 130 135 140 His Gln Val Val Asp Asn Ala Leu Lys Ser Val Leu His Pro Asp Phe 145 150 155 160 Gln Leu Ser His Tyr Thr Ala Pro Lys Lys Ile His Ala Asp Asn Ala 165 170 175 Leu Val Ala Gly Tyr Pro Ala Ala Leu Ile Ala Gln Ala Ala Gly Leu 180 185 190 Gly Gly Ser His Phe Ala Leu Asp Ala Ala Cys Ala Ser Ser Cys Tyr 195 200 205 Ser Val Lys Leu Ala Cys Asp Tyr Leu His Thr Gly Lys Ala Asp Met 210 215 220 Met Leu Ala Gly Ala Val Ser Ala Ala Asp Pro Met Phe Val Asn Met 225 230 235 240 Gly Phe Ser Ile Phe Gln Ala Tyr Pro Ala Asn Asn Val His Ala Pro 245 250 255 Phe Asp Gln Asn Ser Gln Gly Leu Phe Ala Gly Glu Gly Ala Gly Met 260 265 270 Met Val Leu Lys Arg Gln Ser Asp Ala Val Arg Asp Gly Asp His Ile 275 280 285 Tyr Ala Ile Ile Lys Gly Gly Ala Leu Ser Asn Asp Gly Lys Gly Glu 290 295 300 Phe Val Leu Ser Pro Asn Thrh Lys Gly Gln Val Leu Val Tyr Glu Arg 305 310 315 320 Ala Tyr Ala Asp Ala Asp Val Asp Pro Ser Thr Val Asp Tyr Ile Glu 325 330 335 Cys His Ala Thr Gly Thr Pro Lys Gly Asp Asn Val Glu Leu Arg Ser 340 345 350 Met Glu Thr Phe Phe Ser Arg Val Asn Asn Lys Pro Leu Leu Gly Ser 355 360 365 Val Lys Ser Asn Leu Gly His Leu Leu Thr Ala Ala Gly Met Pro Gly 370 375 380 Met Thr Lys Ala Met Leu Ala Leu Gly Lys Gly Leu Ile Pro Ala Thr 385 390 395 400 Ile Asn Leu Lys Gln Pro Leu Gln Ser Lys Asn Gly Tyr Phe Thr Gly 405 410 415 Glu Gln Met Pro Thr Thr Thr Val Pro Trp Pro Thr Thr Pro Gly Ala 420 425 430 Lys Thr Asp Lys Pro Arg Thr Ala Gly Val Ser Val Phe Gly Phe Gly 435 440 445 Gly Ser Asn Ala His Leu Val Leu Gln Gln Pro Thr Gln Thr Leu Glu 450 455 460 Thr Asn Phe Ser Val Ala Lys Pro Arg Glu Ser Leu Ala Ile Ile Gly 465 470 475 475 480 Met Asp Ser His Phe Gly Ser Ala Ser Asn Leu Ala Gln Phe Lys Thr 485 490 495 Leu Leu Asn Asn Asn Gln Asn Thr Phe Arg Glu Leu Pro Glu Gln Arg 500 505 510 Trp Lys Gly Met Glu Ser Asn Ala Asn Val Met Gln Ser Leu Gln Leu 515 520 525 Arg Lys Ala Pro Lys Gly Ser Tyr Val Glu Gln Leu Asp Ile Asp Phe 530 535 540 Leu Arg Phe Lys Val Pro Pro Asn Glu Lys Asp Cys Leu Ile Pro Gln 545 550 555 560 Gln Leu Met Met Met Gln Val Ala Asp Asn Ala Ala Lys Asp Ala Gly 565 570 575 Leu Val Glu Gly Arg Asn Val Ala Val Leu Val Ala Met Gly Met Glu 580 585 590 Leu Glu Leu His Gln Tyr Arg Gly Arg Val Asn Leu Thr Thr Gln Ile 595 600 60 5 Glu Asp Ser Leu Leu Gln Gln Gly Ile Asn Leu Thr Ala Glu Gln Arg 610 615 620 Glu Glu Leu Thr Asn Ile Ala Lys Asp Gly Val Ala Ser Ala Ala Gln 625 630 635 640 Leu Asn Gln Tyr Thr Ser Phe Ile Gly Asn Ile Met Ala Ser Arg Ile 645 650 655 Ser Ala Leu Trp Asp Phe Ser Gly Pro Ala Ile Thr Val Ser Ala Glu 660 665 670 Glu Asn Ser Val Tyr Arg Cys Val Glu Leu Ala Glu Asn Leu Phe Gln 675 680 685 Thr Ser Asp Val Glu Ala Val Ile Ile Ala Ala Val Asp Leu Ser Gly 690 695 700 Ser Ile Glu Asn Ile Thr Leu Arg Gln His Tyr Gly Pro Val Asn Glu 705 710 715 720 Lys Gly Pro Val Ser Glu Cys Gly Pro Val Ser Glu Asn Gly Ser Val 725 730 735 Asn Gly Ser Gly Ser Val Thr Asn Asn Ile Leu Asp Gln Gln Gln Trp 740 745 750 Leu Val Gly Glu Gly Ala Ala Ala Ala Ile Val Val Lys Pro Ser Ser Gln 755 760 765 Val Thr Ala Glu Gln Val Tyr Ala Arg Ile Asp Ala Val Ser Phe Ala 770 775 780 Pro Gly Ser Asp Ala Lys Ala Ile Thr Ile Ala Ala Asp Lys Ala Leu 785 790 790 795 800 Thr Leu Ala Gly Ile Ser Ala Ala Asp Val Ala Ser Val Glu Ala His 805 810 81 5 Ala Ser Gly Phe Ser Ala Glu Asn Asn Ala Glu Lys Thr Ala Leu Pro 820 825 830 Thr Leu Tyr Pro Ser Ala Asn Ile Ser Ser Val Lys Ala Asn Ile Gly 835 840 845 His Thr Phe Asn Ala Ser Gly Met Ala Ser Ile Ile Lys Thr Ala Leu 850 855 860 Leu Leu Asp Gln Asn Thr Ser Gln Asp Gln Lys Ser Gln His Ile Ala 865 870 875 880 Ile Asn Gly Leu Gly Arg Asp Asn Ser Cys Ala His Leu Ile Leu Ser 885 890 895 Ser Ser Glu Gln Ala His Gln Ala Ala Pro Ala Pro Val Ser Gly Met 900 905 910 Ala Lys Gln Arg Pro Gln Leu Val Lys Thr Ile Lys Leu Gly Gly Gln 915 920 925 Leu Ile Ser Asn Ala Ile Val Asn Ser Ala Ser Ser Ser Leu Asp Ala 930 935 940 940 Ile Lys Ala Gln Phe Ala Gly Lys His Leu Asn Lys Val Asn Gln Pro 945 950 955 960 Val Met Met Asp Asn Leu Lys Pro Gln Gly Ile Arg Ala His Ser Met 965 970 975 Asn Glu His Val Val Thr Arg Pro Ser Ser Thr Gln Val Ser His Thr 980 985 990 Gln Ala Phe Thr Gln Ala Ser Ile Gln Ala Ser Ile Gln Glu Gln Glu 995 1000 1005 Leu Ala Gln Glu Gln Val Gln Asn Met Gln Ala Thr Ala Ala Ala Val 1010 1015 1 020 Ser Ser Pro Leu Ser Gln His Gln His Thr Thr Gln Pro Val Ala Ala 1025 1030 1035 1040 Pro Ser Val Val Gly Val Thr Val Lys His Lys Ala Ser Asn Gln Ile 1045 1050 1055 His Gln Gln Ala Ser Thr His Lys Ala Phe Leu Glu Ser Arg Leu Ala 1060 1065 1070 Ala Gln Lys Asn Leu Ser Gln Leu Val Glu Leu Gln Thr Lys Leu Ser 1075 1080 1085 Thr Gln Thr Ala Ser Asp Asn Thr Ser Asn Asn Thr Ser Ser Asn Thr 1090 1095 1100 Val Leu Thr Asn Pro Val Ser Ala Thr Ala Thr Pro Leu Thr Leu Val 1105 1110 1115 1120 Ser Thr Leu Val Thr Asn Lys Pro Val Val Val Thr Ser Leu Thr Ser 1125 1130 1135 Thr Glu Ala Lys Ala Gln Ala Ala Ala Thr Gln Ala Gly Phe Gln Ile 1140 1145 1150 Lys Gly Pro Val Gly Tyr Asn Tyr Pro Pro Leu Gln Leu Ile Glu Arg 1155 1160 1165 Tyr Asn Lys Pro Glu Lys Val Ile Tyr Asp Gln Ala Asp Leu Val Glu 1170 1175 1180 Phe Ala Glu Gly Asp Ile Gly Lys Val Phe Gly Ala Glu Tyr Asn Ile 1185 1190 1195 1200 Ile Asp Gly Tyr Ser Arg Arg Val Arg Leu Pro Thr Ser Asp Tyr Leu 1205 1210 1215 Leu Val Thr Arg Val Thr Glu Leu A sp Ala Lys Val His Glu Tyr Lys 1220 1225 1230 Lys Ser Tyr Met Cys Thr Glu Tyr Asp Val Pro Val Asp Ala Pro Phe 1235 1240 1245 Leu Ile Asp Gly Gln Ile Pro Trp Ser Val Ala Val Glu Ser Gly Gln 1250 1255 1260 Cys Asp Leu Met Leu Ile Ser Tyr Ile Gly Ile Asp Phe Gln Ala Lys 1265 1270 1275 1280 Gly Glu Arg Val Tyr Arg Leu Leu Asp Cys Glu Leu Thr Phe Leu Glu 1285 1290 1295 Glu Met Ala Phe Gly Gly Asp Thr Leu Arg Tyr Glu Ile His Ile Asp 1300 1305 1310 Ser Tyr Ala Arg Asn Gly Glu Gln Leu Leu Phe Phe Phe His Tyr Asp 1315 1320 1325 Cys Tyr Val Gly Asp Lys Lys Val Leu Ile Met Arg Asn Gly Cys Ala 1330 1335 1340 Gly Phe Phe Thr Asp Glu Glu Leu Ser Asp Gly Lys Gly Val Ile His 1345 1350 1355 1360 Asn Asp Lys Asp Lys Ala Glu Phe Ser Asn Ala Val Lys Ser Ser Phe 1365 1370 1375 Thr Pro Leu Leu Gln His Asn Arg Gly Gln Tyr Asp Tyr Asn Asp Met 1380 1385 1390 Met Lys Leu Val Asn Gly Asp Val Ala Ser Cys Phe Gly Pro Gln Tyr 1395 1400 1405 Asp Gln Gly Gly Arg Asn Pro Ser Leu Lys Phe Ser Ser Glu Lys Phe 1410 1415 1 420 Leu Met Ile Glu Arg Ile Thr Lys Ile Asp Pro Thr Gly Gly His Trp 1425 1430 1435 1440 Gly Leu Gly Leu Leu Glu Gly Gln Lys Asp Leu Asp Pro Glu His Trp 1445 1450 1455 Tyr Phe Pro Cys His Phe Lys Gly Asp Gln Val Met Ala Gly Ser Leu 1460 1465 1470 Met Ser Glu Gly Cys Gly Gln Met Ala Met Phe Phe Met Leu Ser Leu 1475 1480 1485 Gly Met His Thr Asn Val Asn Asn Ala Arg Phe Gln Pro Leu Pro Gly 1490 1495 1500 Glu Ser Gln Thr Val Arg Cys Arg Gly Gln Val Leu Pro Gln Arg Asn 1505 1510 1515 1520 Thr Leu Thr Tyr Arg Met Glu Val Thr Ala Met Gly Met His Pro Gln 1525 1530 1535 Pro Phe Met Lys Ala Asn Ile Asp Ile Leu Leu Asp Gly Lys Val Val 1540 1545 1550 Val Asp Phe Lys Asn Leu Ser Val Met Ile Ser Glu Gln Asp Glu His 1555 1560 1565 Ser Asp Tyr Pro Val Thr Leu Pro Ser Asn Val Gln Leu Lys Thr Ile 1570 1575 1580 Ser Ser Ala Ala Val Thr Pro Val Ala Val Thr Thr Thr Pro Val Ala 1585 1590 1595 1600 Leu Ala Thr Thr Asn Asn Ala Asp Leu Asp Glu Arg Gly Val Glu Pro 1605 1610 1615 Phe Lys Tyr Pro Glu Arg Pro Leu M et Arg Val Glu Ser Asp Leu Ser 1620 1625 1630 Ala Pro Lys Ser Lys Gly Val Thr Pro Ile Lys His Phe Glu Ala Pro 1635 1640 1645 Val Val Ala Gly His His Arg Val Pro Asn Gln Ala Pro Phe Thr Pro 1650 1655 1660 Trp His Met Phe Glu Phe Ala Thr Gly Asn Ile Ser Asn Cys Phe Gly 1665 1670 1675 1680 Pro Asp Phe Asp Val Tyr Glu Gly Arg Ile Pro Pro Arg Thr Pro Cys 1685 1690 1695 Gly Asp Leu Gln Val Val Thr Gln Val Val Glu Val Gln Gly Glu Arg 1700 1705 1710 Leu Asp Leu Lys Asn Pro Ser Ser Cys Ile Ala Glu Tyr Tyr Val Pro 1715 1720 1725 Glu Asp Ala Trp Tyr Phe Thr Lys Asn Ser His Glu Asn Trp Met Pro 1730 1735 1740 Tyr Ser Leu Ile Met Glu Ile Ala Leu Gln Pro Asn Gly Phe Ile Ser 1745 1750 1755 1760 Gly Tyr Met Gly Thr Thr Leu Lys Tyr Pro Glu Lys Asp Leu Phe Phe 1765 1770 1775 Arg Asn Leu Asp Gly Ser Gly Thr Leu Leu Lys Gln Ile Asp Leu Arg 1780 1785 1790 Gly Lys Thr Ile Val Asn Lys Ser Val Leu Val Ser Thr Ala Ile Ala 1795 1800 1805 Gly Gly Ala Ile Ile Gln Ser Phe Thr Phe Asp Met Ser Val Asp Gly 1810 1815 1 820 Glu Leu Phe Tyr Thr Gly Lys Ala Val Phe Gly Tyr Phe Ser Gly Glu 1825 1830 1835 1840 Ser Leu Thr Asn Gln Leu Gly Ile Asp Asn Gly Lys Thr Thr Asn Ala 1845 1850 1855 Trp Phe Val Asp Asn Asn Thr Pro Ala Ala Asn Ile Asp Val Phe Asp 1860 1865 1870 Leu Thr Asp Gln Ser Leu Ala Leu Tyr Lys Ala Pro Val Gly Lys Pro 1875 1880 1885 His Tyr Lys Leu Ala Gly Gly Gln Met Asn Phe Ile Asp Thr Val Ser 1890 1895 1900 Val Val Glu Gly Gly Gly Lys Ala Asp Val Ala Tyr Val Tyr Gly Glu 1905 1910 1915 1920 Arg Thr Ile Asp Ala Asp Asp Trp Phe Phe Arg Tyr His Phe His Gln 1925 1930 1935 Asp Pro Val Met Pro Gly Ser Leu Gly Val Glu Ala Ile Ile Glu Leu 1940 1945 1950 Met Gln Thr Tyr Ala Leu Lys Asn Asp Leu Gly Ala Gln Phe Thr Ser 1955 1960 1965 Pro Arg Phe Val Ala Pro Met Thr Lys Val Asp Trp Lys Tyr Arg Gly 1970 1975 1980 Gln Ile Thr Pro Leu Asn Lys Gln Met Ser Leu Asp Val His Ile Thr 1985 1990 1995 2000 Glu Ile Ile Thr Glu Ser Gly Glu Val Arg Leu Val Gly Asp Ala Asn 2005 2010 2015 Leu Ser Lys Asp Gly Leu Arg Ile T yr Glu Val Lys Asn Ile Val Leu 2020 2025 2030 Ser Ile Val Glu Ala 2035

【００５０】 <210> 8 <211> 1614 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 atgtcgagtt taggttttaa caatagcaat gccatcaatt gggcttggaa agtagatcca 60 gcgtcagttc atacacagga tgttggcatt aaagcagctt taatggatct gactaatccg 120 ctttacgtgg ctaataacgc aggtgttaca ggtataacga atcatacgtc agtcgcgggt 180 gctattagca ataatgttga cgttgatgta ctggcgtttg cacaaaagct aacacctgaa 240 gatctaggtg atgatgcgta caagaaacaa catggggtta aatatgcata tcatggcggc 300 gcaatggcta acggtatcgc gtcggttgaa cttgttgtcg cactaggtaa agcagggttg 360 ttatgttcgt tcggcgcggc aggtttagta cctgacgcgg ttgaagatgc tattcgtcgt 420 attcaagctg aattaccaaa tggcccttac gcggttaact tgatccatgc accagcagaa 480 gaagcattag agcgtggggc tgttgaacgt ttccttaaac tgggtgttaa gacggtagag 540 gcttcagctt atcttggttt aactgagcac attgtttggt atcgtgttgc tggtttatct 600 aaaaacagtg atggctcggt taatattggt aacaaggtta tcgctaaagt atcgcgtact 660 gaagttggtc gccgctttat ggaaccagca ccgcaaaagt tactggataa gttattagca 720 caaaacaaga tcacccctga acaagctgct ctagcgctgc ttgtacctat ggctgatgac 780 attactgctg aagctgattc tggtggccat actgacaacc gtccattttt aactttatta 840 ccgactatca tcggactgcg tgatgaagtg caagcgaagt ataacttctc tcctgcatta 900 cgtgttggtg ctggtggcgg tatcggaacg cctgaagcag cactcgctgc atttaacatg 960 ggcgctgctt atatcgttct gggttctgtg aatcaggcgt gtgttgaagc gggtgcatct 1020 gaatatacgc gtaaactgtt atcgacagtt gaaatggccg atgtgactat ggcacctgct 1080 gctgatatgt ttgaaatggg cgtaaagcta caagtattaa aacgtggttc tatgttcgct 1140 atgcgtgcga agaaactgta tgacttgtat gtgacttatg actcgattga agatatccca 1200 gctgcggaac gtgagaagat tgaaaaacaa atcttccgtg caaacctaga cgagatctgg 1260 gatggcacta tcgctttctt cactgaacgt gatccagaaa tgctagcccg tgcaatgagc 1320 agccctaaac gtaaaatggc gcttatcttc cgttggtatc ttggtctttc ttcacgctgg 1380 tcaaacaccg gtgagaaggg acgtgaaatg gattatcaaa tttgggctgg cccaagtcta 1440 ggcgcattta acagctgggt gaaaggttct taccttgaag actatacccg ccgcggcgca 1500 gttgatgttg ctttgcatat gcttaaaggt gctgcgtact tacaacgtgt gaaccagttg 1560 aagctgcaag gcgttagctt aagtacagaa ttagcaagtt atcgtactag tgat 1614<210> 8 <211> 1614 <212> DNA <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> aggtgttaca ggtataacga atcatacgtc agtcgcgggt 180 gctattagca ataatgttga cgttgatgta ctggcgtttg cacaaaagct aacacctgaa 240 gatctaggtg atgatgcgta caagaaacaa catggggtta aatatgcata tcatggcggc 300 gcaatggcta acggtatcgc gtcggttgaa cttgttgtcg cactaggtaa agcagggttg 360 ttatgttcgt tcggcgcggc aggtttagta cctgacgcgg ttgaagatgc tattcgtcgt 420 attcaagctg aattaccaaa tggcccttac gcggttaact tgatccatgc accagcagaa 480 gaagcattag agcgtggggc tgttgaacgt ttccttaaac tgggtgttaa gacggtagag 540 gcttcagctt atcttggttt aactgagcac attgtttggt atcgtgttgc tggtttatct 600 aaaaacagtg atggctcggt taatattggt aacaaggtta tcgctaaagt atcgcgtact 660 gaagttggtc gccgctttat ggaaccagca ccgcaaaagt tactggataa gttattagca 720 caaaacaaga tcagc ctcgcc cttatgca gttattagca 720 caaaacaaga tcaccgcc gatgac 780 attactgctg aagctgattc tggtggccat actgacaacc gtccattttt aactttatta 840 ccgactatca tcggactgcg tgatgaagtg caagcgaagt ataacttctc tcctgcatta 900 cgtgttggtg ctggtggcgg tatcggaacg cctgaagcag cactcgctgc atttaacatg 960 ggcgctgctt atatcgttct gggttctgtg aatcaggcgt gtgttgaagc gggtgcatct 1020 gaatatacgc gtaaactgtt atcgacagtt gaaatggccg atgtgactat ggcacctgct 1080 gctgatatgt ttgaaatggg cgtaaagcta caagtattaa aacgtggttc tatgttcgct 1140 atgcgtgcga agaaactgta tgacttgtat gtgacttatg actcgattga agatatccca 1200 gctgcggaac gtgagaagat tgaaaaacaa atcttccgtg caaacctaga cgagatctgg 1260 gatggcacta tcgctttctt cactgaacgt gatccagaaa tgctagcccg tgcaatgagc 1320 agccctaaac gtaaaatggc gcttatcttc cgttggtatc ttggtctttc ttcacgctgg 1380 tcaaacaccg gtgagaaggg acgtgaaatg gattatcaaa tttgggctgg cccaagtcta 1440 ggcgcattta acagctgggt gaaaggttct taccttgaag actatacccg ccgcggcgca 1500 gttgatgttg ctttgcatat gcttaaaggt gctgcgtact tacaacgtgt gaaccagttg 1560 aagctgcaag gcgttagctt aagtacagaa ttagcaagtt atcgtactag tgat 1614

【００５１】 <210> 9 <211> 538 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 atg tcg agt tta ggt ttt aac aat agc aat gcc atc aat tgg gct tgg 48 Met Ser Ser Leu Gly Phe Asn Asn Ser Asn Ala Ile Asn Trp Ala Trp 1 5 10 15 aaa gta gat cca gcg tca gtt cat aca cag gat gtt ggc att aaa gca 96 Lys Val Asp Pro Ala Ser Val His Thr Gln Asp Val Gly Ile Lys Ala 20 25 30 gct tta atg gat ctg act aat ccg ctt tac gtg gct aat aac gca ggt 144 Ala Leu Met Asp Leu Thr Asn Pro Leu Tyr Val Ala Asn Asn Ala Gly 35 40 45 gtt aca ggt ata acg aat cat acg tca gtc gcg ggt gct att agc aat 192 Val Thr Gly Ile Thr Asn His Thr Ser Val Ala Gly Ala Ile Ser Asn 50 55 60 aat gtt gac gtt gat gta ctg gcg ttt gca caa aag cta aca cct gaa 240 Asn Val Asp Val Asp Val Leu Ala Phe Ala Gln Lys Leu Thr Pro Glu 65 70 75 80 gat cta ggt gat gat gcg tac aag aaa caa cat ggg gtt aaa tat gca 288 Asp Leu Gly Asp Asp Ala Tyr Lys Lys Gln His Gly Val Lys Tyr Ala 85 90 95 tat cat ggc ggc gca atg gct aac ggt atc gcg tcg gtt gaa ctt gtt 336 Tyr His Gly Gly Ala Met Ala Asn Gly Ile Ala Ser Val Glu Leu Val 100 105 110 gtc gca cta ggt aaa gca ggg ttg tta tgt tcg ttc ggc gcg gca ggt 384 Val Ala Leu Gly Lys Ala Gly Leu Leu Cys Ser Phe Gly Ala Ala Gly 115 120 125 tta gta cct gac gcg gtt gaa gat gct att cgt cgt att caa gct gaa 432 Leu Val Pro Asp Ala Val Glu Asp Ala Ile Arg Arg Ile Gln Ala Glu 130 135 140 tta cca aat ggc cct tac gcg gtt aac ttg atc cat gca cca gca gaa 480 Leu Pro Asn Gly Pro Tyr Ala Val Asn Leu Ile His Ala Pro Ala Glu 145 150 155 160 gaa gca tta gag cgt ggg gct gtt gaa cgt ttc ctt aaa ctg ggt gtt 528 Glu Ala Leu Glu Arg Gly Ala Val Glu Arg Phe Leu Lys Leu Gly Val 165 170 175 aag acg gta gag gct tca gct tat ctt ggt tta act gag cac att gtt 576 Lys Thr Val Glu Ala Ser Ala Tyr Leu Gly Leu Thr Glu His Ile Val 180 185 190 tgg tat cgt gtt gct ggt tta tct aaa aac agt gat ggc tcg gtt aat 624 Trp Tyr Arg Val Ala Gly Leu Ser Lys Asn Ser Asp Gly Ser Val Asn 195 200 205 att ggt aac aag gtt atc gct aaa gta tcg cgt act gaa gtt ggt cgc 672 Ile Gly Asn Lys Val Ile Ala Lys Val Ser Arg Thr Glu Val Gly Arg 210 215 220 cgc ttt atg gaa cca gca ccg caa aag tta ctg gat aag tta tta gca 720 Arg Phe Met Glu Pro Ala Pro Gln Lys Leu Leu Asp Lys Leu Leu Ala 225 230 235 240 caa aac aag atc acc cct gaa caa gct gct cta gcg ctg ctt gta cct 768 Gln Asn Lys Ile Thr Pro Glu Gln Ala Ala Leu Ala Leu Leu Val Pro 245 250 255 atg gct gat gac att act gct gaa gct gat tct ggt ggc cat act gac 816 Met Ala Asp Asp Ile Thr Ala Glu Ala Asp Ser Gly Gly His Thr Asp 260 265 270 aac cgt cca ttt tta act tta tta ccg act atc atc gga ctg cgt gat 864 Asn Arg Pro Phe Leu Thr Leu Leu Pro Thr Ile Ile Gly Leu Arg Asp 275 280 285 gaa gtg caa gcg aag tat aac ttc tct cct gca tta cgt gtt ggt gct 912 Glu Val Gln Ala Lys Tyr Asn Phe Ser Pro Ala Leu Arg Val Gly Ala 290 295 300 ggt ggc ggt atc gga acg cct gaa gca gca ctc gct gca ttt aac atg 960 Gly Gly Gly Ile Gly Thr Pro Glu Ala Ala Leu Ala Ala Phe Asn Met 305 310 315 320 ggc gct gct tat atc gtt ctg ggt tct gtg aat cag gcg tgt gtt gaa 1008 Gly Ala Ala Tyr Ile Val Leu Gly Ser Val Asn Gln Ala Cys Val Glu 325 330 335 gcg ggt gca tct gaa tat acg cgt aaa ctg tta tcg aca gtt gaa atg 1056 Ala Gly Ala Ser Glu Tyr Thr Arg Lys Leu Leu Ser Thr Val Glu Met 340 345 350 gcc gat gtg act atg gca cct gct gct gat atg ttt gaa atg ggc gta 1104 Ala Asp Val Thr Met Ala Pro Ala Ala Asp Met Phe Glu Met Gly Val 355 360 365 aag cta caa gta tta aaa cgt ggt tct atg ttc gct atg cgt gcg aag 1152 Lys Leu Gln Val Leu Lys Arg Gly Ser Met Phe Ala Met Arg Ala Lys 370 375 380 aaa ctg tat gac ttg tat gtg act tat gac tcg att gaa gat atc cca 1200 Lys Leu Tyr Asp Leu Tyr Val Thr Tyr Asp Ser Ile Glu Asp Ile Pro 385 390 395 400 gct gcg gaa cgt gag aag att gaa aaa caa atc ttc cgt gca aac cta 1248 Ala Ala Glu Arg Glu Lys Ile Glu Lys Gln Ile Phe Arg Ala Asn Leu 405 410 415 gac gag atc tgg gat ggc act atc gct ttc ttc act gaa cgt gat cca 1296 Asp Glu Ile Trp Asp Gly Thr Ile Ala Phe Phe Thr Glu Arg Asp Pro 420 425 430 gaa atg cta gcc cgt gca atg agc agc cct aaa cgt aaa atg gcg ctt 1344 Glu Met Leu Ala Arg Ala Met Ser Ser Pro Lys Arg Lys Met Ala Leu 435 440 445 atc ttc cgt tgg tat ctt ggt ctt tct tca cgc tgg tca aac acc ggt 1392 Ile Phe Arg Trp Tyr Leu Gly Leu Ser Ser Arg Trp Ser Asn Thr Gly 450 455 460 gag aag gga cgt gaa atg gat tat caa att tgg gct ggc cca agt cta 1440 Glu Lys Gly Arg Glu Met Asp Tyr Gln Ile Trp Ala Gly Pro Ser Leu 465 470 475 480 ggc gca ttt aac agc tgg gtg aaa ggt tct tac ctt gaa gac tat acc 1488 Gly Ala Phe Asn Ser Trp Val Lys Gly Ser Tyr Leu Glu Asp Tyr Thr 485 490 495 cgc cgc ggc gca gtt gat gtt gct ttg cat atg ctt aaa ggt gct gcg 1536 Arg Arg Gly Ala Val Asp Val Ala Leu His Met Leu Lys Gly Ala Ala 500 505 510 tac tta caa cgt gtg aac cag ttg aag ctg caa ggc gtt agc tta agt 1584 Tyr Leu Gln Arg Val Asn Gln Leu Lys Leu Gln Gly Val Ser Leu Ser 515 520 525 aca gaa tta gca agt tat cgt act agt gat 1614 Thr Glu Leu Ala Ser Tyr Arg Thr Ser Asp 530 535 <210> 9 <211> 538 <212> PRT <213> Shewanella sp.SCRC-21406 (FERM BP-5979) <400> 1 atg tcg agt tta ggt ttt aac aat agc aat gcc atc aat tgg gct tgg 48 Met Ser Ser Leu Gly Phe Asn Asn Ser Asn Ala Ile Asn Trp Ala Trp 1 5 10 15 aaa gta gat cca gcg tca gtt cat aca cag gat gtt ggc att aaa gca 96 Lys Val Asp Pro Ala Ser Val His Thr Gln Asp Val Gly Ile Lys Ala 20 25 30 gct tta atg gat ctg act aat ccg ctt tac gtg gct aat aac gca ggt 144 Ala Leu Met Asp Leu Thr Asn Pro Leu Tyr Val Ala Asn Asn Ala Gly 35 40 45 gtt aca ggt ata acg aat cat acg tca gtc gcg ggt gct att agc aat 192 Val Thr Gly Ile Thr Asn His Thr Ser Val Ala Gly Ala Ile Ser Asn 50 55 60 aat gtt gac gtt gat gta ctg gcg ttt gca caa aag cta aca cct gaa 240 Asn Val Asp Val Asp Val Leu Ala Phe Ala Gln Lys Leu Thr Pro Glu 65 70 75 80 gat cta ggt gat gat gcg tac aag aaa caa cat ggg gtt aaa tat gca 288 Asp Leu Gly Asp Asp Ala Tyr Lys Lys Gln His Gly Val Lys Tyr Ala 85 90 95 tat cat ggc ggc gca atg gct aac ggt atc gcg tcg gtt gaa ctt gtt 336 Tyr His Gly Gly Ala Met Ala Asn Gly Ile Ala Ser Val Glu Leu Val 100 105 110 gtc gca cta ggt aaa gca ggg ttg tta tgt tcg ttc ggc gcg gca ggt 384 Val Ala Leu Gly Lys Ala Gly Leu Cy Ser Phe Gly Ala Ala Gly 115 120 125 tta gta cct gac gcg gtt gaa gat gct att cgt cgt att caa gct gaa 432 Leu Val Pro Asp Ala Val Glu Asp Ala Ile Arg Arg Ile Gln Ala Glu 130 135 140 tta cca aat ggc cct tac gcg gtt aac ttg atc cat gca cca gca gaa 480 Leu Pro Asn Gly Pro Tyr Ala Val Asn Leu Ile His Ala Pro Ala Glu 145 150 155 160 gaa gca tta gag cgt ggg gct gtt gaa cgt ttc ctt aaa ctg ggt gtt 528 Ala Leu Glu Arg Gly Ala Val Glu Arg Phe Leu Lys Leu Gly Val 165 170 175 aag acg gta gag gct tca gct tat ctt ggt tta act gag cac att gtt 576 Lys Thr Val Glu Ala Ser Ala Tyr Leu Gly Leu Thr Glu His Ile Val 180 185 190 tgg tat cgt gtt gct ggt tta tct aaa aac agt gat ggc tcg gtt aat 624 Trp Tyr Arg Val Ala Gly Leu Ser Lys Asn Ser Asp Gly Ser Val Asn 195 200 205 att ggt aac aag gtt atc gct aaa gta tcg cgt act gaa gtt ggt cgc 672 Ile Gly Asn Lys Val Ile Ala Lys Val Ser Arg Thr Glu Val Gly Arg 210 215 220 cgc ttt atg gaa cca gca ccg caa aag tta ctg gat aag tta tta gca 720 Arg Phe Met Glu Pro Ala Pro Gln Lys Leu Leu Asp Lys Leu Leu Ala 225 230 235 240 caa aac aag atc acc cct gaa caa gct gct cta gcg ctg ctt gta cct 768 Gln Asn Lys Ile Thr Pro Glu Gln Ala Ala Leu Ala Leu Leu Val Pro 245 250 255 atg gct gat gac att act gct gaa gct gat tct ggt ggc cat act gac 816 Met Ala Asp Asp Ile Thr Ala Glu Ala Asp Ser Gly Gly His Thr Asp 260 265 270 aac cgt cca ttt tta act tta tta ccg act atc atc gga ctg cgt gat 864 Asn Arg Pro Phe Leu Thr Leu Leu Pro Thr Ile Ile Gly Leu Arg Asp 275 280 285 gaa gtg caa gcg aag tat aac ttc tct cct gca tta cgt gtt ggt gct 912 Glu Val Gln Ala Lys Tyr Asn Phe Ser Ala Leu Arg Val Gly Ala 290 295 300 ggt ggc ggt atc gga acg cct gaa gca gca ctc gct gca ttt aac atg 960 Gly Gly Gly Ile Gly Thr Pro Glu Ala Ala Leu Ala Ala Phe Asn Met 305 310 315 320 ggc gct gct tat atc g ctg ggt tct gtg aat cag gcg tgt gtt gaa 1008 Gly Ala Ala Tyr Ile Val Leu Gly Ser Val Asn Gln Ala Cys Val Glu 325 330 335 gcg ggt gca tct gaa tat acg cgt aaa ctg tta tcg aca gtt Ala atg10 Ser Glu Tyr Thr Arg Lys Leu Leu Ser Thr Val Glu Met 340 345 350 gcc gat gtg act atg gca cct gct gct gat atg ttt gaa atg ggc gta 1104 Ala Asp Val Thr Met Ala Pro Ala Ala Asp Met Phe Glu Met Gly Val 355 360 365 aag cta caa gta tta aaa cgt ggt tct atg ttc gct atg cgt gcg aag 1152 Lys Leu Gln Val Leu Lys Arg Gly Ser Met Phe Ala Met Arg Ala Lys 370 375 380 aaa ctg tat gac ttg tat gtg tact gat gac tat gaa gat atc cca 1200 Lys Leu Tyr Asp Leu Tyr Val Thr Tyr Asp Ser Ile Glu Asp Ile Pro 385 390 395 400 gct gcg gaa cgt gag aag att gaa aaa caa atc ttc cgt gca aac cta 1248 Ala Ala Glu Arg Glu Lysle Lys Gln Ile Phe Arg Ala Asn Leu 405 410 415 gac gag atc tgg gat ggc act atc gct ttc ttc act gaa cgt gat cca 1296 Asp Glu Ile Trp Asp Gly Thr Ile Ala Phe Phe Thr Glu Arg Asp Pro 420 425 430 gaa atg cta gcc cgt gca atg agc agc cct aaa cgt aaa atg gcg ctt 1344 Glu Met Leu Ala Arg Ala Met Ser Ser Pro Lys Arg Lys Met Ala Leu 435 440 445 atc ttc cgt tgg tat ctt ggt ctt tct tca cgc tgg tca ggt 1392 Ile Phe Arg Trp Tyr Leu Gly Leu Ser Ser Arg Trp Ser Asn Thr Gly 450 455 460 gag aag gga cgt gaa atg gat tat caa att tgg gct ggc cca agt cta 1440 Glu Lys Gly Arg Glu Met Asp Tyr Gln Ile Trp Ala Gly Pro Ser Leu 465 470 475 480 ggc gca ttt aac agc tgg gtg aaa ggt tct tac ctt gaa gac tat acc 1488 Gly Ala Phe Asn Ser Trp Val Lys Gly Ser Tyr Leu Glu Asp Tyr Thr 485 490 495 495 cgc cgc ggc gca gtt gtt gct ttg cat atg ctt aaa ggt gct gcg 1536 Arg Arg Gly Ala Val Asp Val Ala Leu His Met Leu Lys Gly Ala Ala 500 505 510 tac tta caa cgt gtg aac cag ttg aag ctg caa ggc gtt agc tta agt 1yr Tyr Arg Val Asn Gln Leu Lys Leu Gln Gly Val Ser Leu Ser 515 520 525 aca gaa tta gca agt tat cgt act agt gat 1614 Thr Glu Leu Ala Ser Tyr Arg Thr Ser Asp 530 535

【００５２】[0052]

【図面の簡単な説明】[Brief description of the drawings]

【図１】コスミドp10C7の制限酵素地図を示す。図面の
太線部分はドコサヘキサエン酸産生菌の染色体由来の遺
伝子断片を示す。太線内の白色部分は塩基配列を決定し
た部分を示す。FIG. 1 shows a restriction map of cosmid p10C7. The bold line in the drawing indicates a gene fragment derived from the chromosome of the docosahexaenoic acid-producing bacterium. The white part in the bold line indicates the part where the base sequence was determined.

【図２】コスミドp10C7のサブクローンの挿入断片の位
置を示す。FIG. 2 shows the position of the inserted fragment of the cosmid p10C7 subclone.

【図３】ＥＰＡ生合成系遺伝子群のサブクローンの挿入
断片の位置を示す。FIG. 3 shows the position of the inserted fragment of the subclone of the EPA biosynthesis gene group.

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.⁷ 識別記号 ＦＩ テーマコート゛(参考） (Ｃ１２Ｎ 1/21 Ｃ１２Ｒ 1:19） (72)発明者 山田 章子 神奈川県相模原市若松６−１−27 Ｂ− 305 (72)発明者 湯 玲子 神奈川県横浜市緑区霧が丘３−24−４− 108 (72)発明者 渡部 和郎 神奈川県相模原市南台４−３−２ (72)発明者 照屋 潤二郎 神奈川県相模原市西大沼４−４−１ (72)発明者 鎌田 正純 神奈川県相模原市星が丘１−15−16 (72)発明者 倉根 隆一郎 茨城県つくば市東１丁目１番３ 工業技術 院生命工学工業技術研究所内──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) (C12N 1/21 C12R 1:19) (72) Inventor Akiko Yamada 6-1-27 Wakamatsu, Sagamihara-shi, Kanagawa B-305 (72) Inventor Reiko Yu 3-24-4-108, Kirigaoka, Midori-ku, Yokohama-shi, Kanagawa Prefecture (72) Inventor Kazuo Watanabe 4-3-2, Minamidai, Sagamihara-shi, Kanagawa Prefecture (72) Inventor Junjiro Teriya Kanagawa Prefecture 4-4-1 Nishi Onuma, Sagamihara City (72) Inventor Masazumi Kamada 1-15-16, Hoshigaoka, Sagamihara City, Kanagawa Prefecture (72) Inventor Ryuichiro Kurane 1-3-3 Higashi, Tsukuba City, Ibaraki Pref. In the laboratory

Claims (7)

【特許請求の範囲】[Claims] 【請求項１】ドコサヘキサエン酸産生菌の染色体DNAをS
au3AIで処理して得られる、図１で示される制限酵素地
図を有する約37kbの遺伝子断片に含まれ、イコサペンタ
エン酸及び／又はドコサヘキサエン酸の生合成に関与す
る酵素群をコードする遺伝子。1. The chromosomal DNA of a docosahexaenoic acid-producing bacterium is
A gene which is contained in a gene fragment of about 37 kb having a restriction map shown in FIG. 1 and which is obtained by treating with au3AI and which encodes a group of enzymes involved in biosynthesis of icosapentaenoic acid and / or docosahexaenoic acid. 【請求項２】ドコサヘキサエン酸産生菌がシーワネラs
p. SCRC-21406(FERM BP-5979)である、請求項１に記載
の遺伝子。2. The method according to claim 1, wherein the docosahexaenoic acid-producing bacterium is Seawanella s.
The gene according to claim 1, which is p. SCRC-21406 (FERM BP-5979). 【請求項３】配列番号：１で示される塩基配列によって
コード化されたイコサペンタエン酸及び／又はドコサヘ
キサエン酸の生合成に関与する酵素群をコードする遺伝
子。3. A gene encoding an enzyme group involved in biosynthesis of icosapentaenoic acid and / or docosahexaenoic acid, which is encoded by the nucleotide sequence represented by SEQ ID NO: 1. 【請求項４】配列番号：２，４，６及び８で示される塩
基配列群から選ばれた少なくとも一つの塩基配列を含ん
で成るイコサペンタエン酸及び／又はドコサヘキサエン
酸の生合成に関与する酵素群をコードする遺伝子。4. An enzyme group involved in the biosynthesis of icosapentaenoic acid and / or docosahexaenoic acid comprising at least one base sequence selected from the base sequence groups represented by SEQ ID NOs: 2, 4, 6, and 8. Gene to encode. 【請求項５】配列番号：３，５，７及び９で示されるア
ミノ酸配列群から選ばれた少なくとも一つのアミノ酸配
列を含んで成るイコサペンタエン酸及び／又はドコサヘ
キサエン酸の生合成に関与する酵素群をコードする遺伝
子。5. An enzyme group involved in the biosynthesis of icosapentaenoic acid and / or docosahexaenoic acid, comprising at least one amino acid sequence selected from the amino acid sequences represented by SEQ ID NOs: 3, 5, 7, and 9. Gene to encode. 【請求項６】請求項３または４に記載の遺伝子を含んで
成るプラスミド。6. A plasmid comprising the gene according to claim 3 or 4. 【請求項７】請求項６に記載のプラスミドにより形質転
換された微生物。7. A microorganism transformed by the plasmid according to claim 6.