IT201600117326A1 - Linee cellulari stabili per la produzione retrovirale. - Google Patents

Linee cellulari stabili per la produzione retrovirale.

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Publication number
IT201600117326A1
IT201600117326A1 IT102016000117326A IT201600117326A IT201600117326A1 IT 201600117326 A1 IT201600117326 A1 IT 201600117326A1 IT 102016000117326 A IT102016000117326 A IT 102016000117326A IT 201600117326 A IT201600117326 A IT 201600117326A IT 201600117326 A1 IT201600117326 A1 IT 201600117326A1
Authority
IT
Italy
Prior art keywords
cell lines
stable cell
retroviral production
retroviral
production
Prior art date
Application number
IT102016000117326A
Other languages
English (en)
Inventor
Sabine Johnson
Celeste Pallant
Eirini Vamva
Conrad Vink
Original Assignee
Glaxosmithkline Ip Dev Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1520761.6A external-priority patent/GB201520761D0/en
Priority claimed from GBGB1609303.1A external-priority patent/GB201609303D0/en
Application filed by Glaxosmithkline Ip Dev Ltd filed Critical Glaxosmithkline Ip Dev Ltd
Publication of IT201600117326A1 publication Critical patent/IT201600117326A1/it

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C12N7/025—Packaging cell lines, e.g. transcomplementing cell lines, for production of virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
    • C12N7/045—Pseudoviral particles; Non infectious pseudovirions, e.g. genetically engineered
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N2740/00—Reverse transcribing RNA viruses
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Seal Device For Vehicle (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
IT102016000117326A 2015-11-24 2016-11-21 Linee cellulari stabili per la produzione retrovirale. IT201600117326A1 (it)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1520761.6A GB201520761D0 (en) 2015-11-24 2015-11-24 Stable cell lines for retroviral production
GBGB1609303.1A GB201609303D0 (en) 2016-05-26 2016-05-26 Stable cell lines for retroviral production

Publications (1)

Publication Number Publication Date
IT201600117326A1 true IT201600117326A1 (it) 2018-05-21

Family

ID=57354376

Family Applications (1)

Application Number Title Priority Date Filing Date
IT102016000117326A IT201600117326A1 (it) 2015-11-24 2016-11-21 Linee cellulari stabili per la produzione retrovirale.

Country Status (17)

Country Link
US (3) US20170145388A1 (it)
EP (2) EP3489353B8 (it)
JP (1) JP7098521B2 (it)
KR (1) KR102091957B1 (it)
CN (1) CN108291208A (it)
AU (1) AU2016360763B2 (it)
BR (1) BR112018010635A2 (it)
CA (1) CA3006288A1 (it)
DE (1) DE102016122316A1 (it)
ES (2) ES2939617T3 (it)
FR (1) FR3044016A1 (it)
GB (1) GB2544892B (it)
IL (2) IL259254A (it)
IT (1) IT201600117326A1 (it)
RU (1) RU2752498C2 (it)
SA (1) SA518391623B1 (it)
WO (1) WO2017089308A1 (it)

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EP3489353C0 (en) 2023-07-26
EP3380604B1 (en) 2022-12-28
IL282603A (en) 2021-06-30
CA3006288A1 (en) 2017-06-01
IL259254A (en) 2018-07-31
WO2017089308A1 (en) 2017-06-01
SA518391623B1 (ar) 2022-10-30
RU2018122636A3 (it) 2020-04-02
RU2752498C2 (ru) 2021-07-28
DE102016122316A1 (de) 2017-05-24
JP7098521B2 (ja) 2022-07-11
KR102091957B1 (ko) 2020-03-20
EP3489353B1 (en) 2023-07-26
GB2544892B (en) 2017-11-15
ES2959326T3 (es) 2024-02-23
FR3044016A1 (fr) 2017-05-26
RU2018122636A (ru) 2019-12-25
GB201619643D0 (en) 2017-01-04
KR20180079351A (ko) 2018-07-10
US20170145388A1 (en) 2017-05-25
EP3489353A1 (en) 2019-05-29
AU2016360763A1 (en) 2018-05-31
CN108291208A (zh) 2018-07-17
US20200123505A1 (en) 2020-04-23
EP3380604A1 (en) 2018-10-03
AU2016360763B2 (en) 2020-11-05
EP3489353B8 (en) 2023-08-30
US20180320147A1 (en) 2018-11-08
JP2018534937A (ja) 2018-11-29
BR112018010635A2 (pt) 2018-11-27
ES2939617T3 (es) 2023-04-25
IL282603B (en) 2022-05-01
GB2544892A (en) 2017-05-31

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