IN2014KN01168A - - Google Patents
Info
- Publication number
- IN2014KN01168A IN2014KN01168A IN1168KON2014A IN2014KN01168A IN 2014KN01168 A IN2014KN01168 A IN 2014KN01168A IN 1168KON2014 A IN1168KON2014 A IN 1168KON2014A IN 2014KN01168 A IN2014KN01168 A IN 2014KN01168A
- Authority
- IN
- India
- Prior art keywords
- aptamer
- analyte
- sam
- metal surface
- complex
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/5695—Mycobacteria
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2610/00—Assays involving self-assembled monolayers [SAMs]
Abstract
An aptamer based SERS detection technique that directly monitors an aptamer analyte capture event by generating spectroscopic information regarding the identity of the analyte that has been bound to the aptamer from a complex biological sample. A reproducible SERS spectrum is measured for an aptamer analyte complex formed on a metal surface and this spectral information is used directly to identify the specific aptamer analyte complex and optionally also to quantify the analyte in the sample thus enabling discrimination between true and false positives in quantitative analyte assays on complex biological samples. In one embodiment the aptamer is attached directly to the metal surface and surrounded by a self assembled monolayer (SAM) of amphiphilic molecules. In an alternative embodiment the metal surface is coated with a SAM and the aptamer is attached to the amphiphilic molecules of the SAM.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ZA201108016 | 2011-11-02 | ||
PCT/IB2012/056108 WO2013065016A1 (en) | 2011-11-02 | 2012-11-02 | A method of detecting and/or quantifying an analyte in a biological sample |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2014KN01168A true IN2014KN01168A (en) | 2015-10-16 |
Family
ID=47222168
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN1168KON2014 IN2014KN01168A (en) | 2011-11-02 | 2012-11-02 |
Country Status (8)
Country | Link |
---|---|
US (1) | US9518986B2 (en) |
EP (1) | EP2773958B1 (en) |
CN (1) | CN104380105B (en) |
BR (1) | BR112014010696A2 (en) |
DK (1) | DK2773958T3 (en) |
IN (1) | IN2014KN01168A (en) |
WO (1) | WO2013065016A1 (en) |
ZA (1) | ZA201403386B (en) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015143247A1 (en) * | 2014-03-19 | 2015-09-24 | The Regents Of The University Of Colorado, A Body Corporate | Binding detection using liquid crystal |
WO2016134214A1 (en) * | 2015-02-19 | 2016-08-25 | Ionica Sciences | Reagents and methods for detecting infectious diseases |
CN105510411B (en) * | 2015-09-14 | 2018-05-08 | 陕西师范大学 | The detection method of single cancer cell is realized in interaction based on cell and microelectrode |
DE102015220414A1 (en) * | 2015-10-20 | 2017-04-20 | Robert Bosch Gmbh | Propofol sensor for measuring anesthesia depth |
CN105277527A (en) * | 2015-10-29 | 2016-01-27 | 江南大学 | Fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles |
KR101816521B1 (en) * | 2016-01-28 | 2018-01-09 | 광주과학기술원 | LSPR-based high sensitivity aptamer sensor using intercalation agent |
US10103357B2 (en) | 2016-02-17 | 2018-10-16 | The Curators Of The University Of Missouri | Fabrication of multilayer nanograting structures |
EP3516557A4 (en) | 2016-10-28 | 2020-05-27 | Hewlett-Packard Development Company, L.P. | Target class feature model |
KR101923196B1 (en) * | 2017-09-25 | 2018-11-28 | 주식회사 엠디엡투스 | DNA aptamer specifically binding to CFP10 and use thereof |
WO2019126182A1 (en) * | 2017-12-18 | 2019-06-27 | The University Of Memphis Research Foundation | Compositions and methods for the detection and molecular profiling of membrane bound vesicles |
TWI686599B (en) * | 2018-05-24 | 2020-03-01 | 國立清華大學 | System and method for detection of dust mite antigens |
WO2020100159A1 (en) * | 2018-11-13 | 2020-05-22 | Translational Health Science And Technology Institute | A novel aptamer and an electrochemical biosensor for the rapid detection and diagnosis of tuberculous meningitis |
TWI693403B (en) * | 2019-01-17 | 2020-05-11 | 逢甲大學 | Immunodetection method |
EP3953686A4 (en) * | 2019-04-09 | 2022-12-21 | Agency for Science, Technology and Research | Label-free detection of mycobacteria using surface enhanced raman spectroscopy |
CN110907643A (en) * | 2019-12-02 | 2020-03-24 | 中国科学院重庆绿色智能技术研究院 | Preparation method of escherichia coli detection chip and detection chip |
TWI759776B (en) * | 2020-06-22 | 2022-04-01 | 淡江大學 | Detection substrate, raman spectrum detection system and raman spectrum detection method |
CA3193691A1 (en) | 2020-09-24 | 2022-03-31 | Roya Khosravi-Far | Systems, apparatus, and methods for detecting pathogens |
CN112240881A (en) * | 2020-09-30 | 2021-01-19 | 合肥国研汉因检测科技有限公司 | Method for rapidly detecting trace fentanyl by using Raman spectroscopy |
CN112986212B (en) * | 2021-03-10 | 2023-01-17 | 中国人民解放军海军军医大学 | Research method for interaction of G-quadruplex aptamer and small molecule |
CN115711873A (en) * | 2022-11-09 | 2023-02-24 | 清华大学 | Method for carrying out quantitative analysis by utilizing SERS file card |
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-
2012
- 2012-11-02 WO PCT/IB2012/056108 patent/WO2013065016A1/en active Application Filing
- 2012-11-02 BR BR112014010696A patent/BR112014010696A2/en not_active Application Discontinuation
- 2012-11-02 DK DK12790972.9T patent/DK2773958T3/en active
- 2012-11-02 IN IN1168KON2014 patent/IN2014KN01168A/en unknown
- 2012-11-02 CN CN201280065543.0A patent/CN104380105B/en not_active Expired - Fee Related
- 2012-11-02 US US14/355,765 patent/US9518986B2/en active Active
- 2012-11-02 EP EP12790972.9A patent/EP2773958B1/en not_active Not-in-force
-
2014
- 2014-05-12 ZA ZA2014/03386A patent/ZA201403386B/en unknown
Also Published As
Publication number | Publication date |
---|---|
US9518986B2 (en) | 2016-12-13 |
EP2773958A1 (en) | 2014-09-10 |
ZA201403386B (en) | 2016-02-24 |
CN104380105A (en) | 2015-02-25 |
DK2773958T3 (en) | 2018-03-12 |
EP2773958B1 (en) | 2017-11-29 |
CN104380105B (en) | 2017-04-12 |
BR112014010696A2 (en) | 2017-04-25 |
US20140302492A1 (en) | 2014-10-09 |
WO2013065016A1 (en) | 2013-05-10 |
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