IL129382A - Method for identifying embryonic cells using antibodies against fetal hemoglobin - Google Patents
Method for identifying embryonic cells using antibodies against fetal hemoglobinInfo
- Publication number
- IL129382A IL129382A IL12938297A IL12938297A IL129382A IL 129382 A IL129382 A IL 129382A IL 12938297 A IL12938297 A IL 12938297A IL 12938297 A IL12938297 A IL 12938297A IL 129382 A IL129382 A IL 129382A
- Authority
- IL
- Israel
- Prior art keywords
- fetal
- antibody
- cells
- hemoglobin
- cell
- Prior art date
Links
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
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- G—PHYSICS
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- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Pregnancy & Childbirth (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Gynecology & Obstetrics (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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IL14569197A IL145691A (en) | 1996-10-21 | 1997-10-20 | Method to identify fetal cells using anti-embryonic hemoglobin antibodies |
Applications Claiming Priority (2)
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US08/734,556 US5731156A (en) | 1996-10-21 | 1996-10-21 | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
PCT/US1997/019447 WO1998018005A1 (en) | 1996-10-21 | 1997-10-20 | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
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IL129382A0 IL129382A0 (en) | 2000-02-17 |
IL129382A true IL129382A (en) | 2002-09-12 |
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Application Number | Title | Priority Date | Filing Date |
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IL12938297A IL129382A (en) | 1996-10-21 | 1997-10-20 | Method for identifying embryonic cells using antibodies against fetal hemoglobin |
Country Status (14)
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US (1) | US5731156A (ru) |
EP (1) | EP1007965B1 (ru) |
JP (1) | JP4091123B2 (ru) |
KR (1) | KR100523381B1 (ru) |
CN (1) | CN1234117A (ru) |
AT (1) | ATE290210T1 (ru) |
AU (1) | AU735380B2 (ru) |
BR (1) | BR9712548A (ru) |
CA (1) | CA2269327A1 (ru) |
DE (1) | DE69732662T2 (ru) |
HK (1) | HK1031255A1 (ru) |
IL (1) | IL129382A (ru) |
RU (1) | RU2178703C2 (ru) |
WO (1) | WO1998018005A1 (ru) |
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US6509192B1 (en) * | 1992-02-24 | 2003-01-21 | Coulter International Corp. | Quality control method |
US5629147A (en) * | 1992-07-17 | 1997-05-13 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
US6718053B1 (en) * | 1996-11-27 | 2004-04-06 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
CA2236268A1 (en) | 1995-11-30 | 1997-06-05 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
PL335543A1 (en) * | 1997-03-08 | 2000-04-25 | Univ Dundee | Prenatal diagnosing methods |
US5962234A (en) * | 1997-10-20 | 1999-10-05 | Applied Imaging Corporation | Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells |
CA2331508A1 (en) * | 1998-05-09 | 1999-11-18 | Ikonisys, Inc. | Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis |
FR2781055B1 (fr) * | 1998-07-09 | 2000-10-13 | Immunotech Sa | Reactif et methode pour la permeabilisation et l'identification des erythrocytes |
CA2413835A1 (en) * | 1999-05-25 | 2000-11-30 | Britta Christensen | Isolation and culturing of fetal cells |
US6692952B1 (en) * | 1999-11-10 | 2004-02-17 | Massachusetts Institute Of Technology | Cell analysis and sorting apparatus for manipulation of cells |
DE10035433C2 (de) * | 2000-07-20 | 2002-07-18 | Tuma Wolfgang | Schonende Hochanreicherung von fetalen Zellen aus pripherem Blut und Verwendung derselben |
AU2002357588B2 (en) * | 2001-12-11 | 2008-11-20 | Netech Inc. | Blood cell separation system |
WO2004029221A2 (en) | 2002-09-27 | 2004-04-08 | The General Hospital Corporation | Microfluidic device for cell separation and uses thereof |
DK1816461T3 (da) | 2002-10-16 | 2020-04-14 | Streck Laboratories Inc | Fremgangsmåde og indretning til indsamling og sikring af celler til brug for analyse |
US20040214243A1 (en) * | 2003-04-25 | 2004-10-28 | Beckman Coulter, Inc. | Differential determination of hemoglobins |
US20070160503A1 (en) * | 2003-06-13 | 2007-07-12 | Palaniappan Sethu | Microfluidic systems for size based removal of red blood cells and platelets from blood |
WO2005084374A2 (en) * | 2004-03-03 | 2005-09-15 | The General Hospital Corporation | Magnetic device for isolation of cells and biomolecules in a microfluidic environment |
US7653260B2 (en) * | 2004-06-17 | 2010-01-26 | Carl Zeis MicroImaging GmbH | System and method of registering field of view |
US8582924B2 (en) * | 2004-06-30 | 2013-11-12 | Carl Zeiss Microimaging Gmbh | Data structure of an image storage and retrieval system |
US20070026415A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070026417A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
US20070026414A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070026413A1 (en) * | 2005-07-29 | 2007-02-01 | Mehmet Toner | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20090181421A1 (en) * | 2005-07-29 | 2009-07-16 | Ravi Kapur | Diagnosis of fetal abnormalities using nucleated red blood cells |
US20070026416A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070059680A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for cell enrichment |
US20070059719A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Business methods for prenatal Diagnosis |
US20070059683A1 (en) * | 2005-09-15 | 2007-03-15 | Tom Barber | Veterinary diagnostic system |
US20070059774A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Kits for Prenatal Testing |
US20070059716A1 (en) * | 2005-09-15 | 2007-03-15 | Ulysses Balis | Methods for detecting fetal abnormality |
US20070059781A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for size based separation and analysis |
US20070059718A1 (en) * | 2005-09-15 | 2007-03-15 | Mehmet Toner | Systems and methods for enrichment of analytes |
EP2156370B1 (en) * | 2007-05-14 | 2011-10-12 | Historx, Inc. | Compartment segregation by pixel characterization using image data clustering |
JP5593221B2 (ja) * | 2007-06-15 | 2014-09-17 | ヒストロックス,インコーポレイテッド. | 顕微鏡機器を標準化するための方法およびシステム |
CA2604317C (en) | 2007-08-06 | 2017-02-28 | Historx, Inc. | Methods and system for validating sample images for quantitative immunoassays |
CA2596204C (en) * | 2007-08-07 | 2019-02-26 | Historx, Inc. | Method and system for determining an optimal dilution of a reagent |
WO2009029810A1 (en) * | 2007-08-31 | 2009-03-05 | Historx, Inc. | Automatic exposure time selection for imaging tissue |
EP2232266B1 (en) | 2007-12-20 | 2012-12-05 | Cytyc Corporation | Method for magnetic separation of red blood cells from a patient sample |
WO2009103110A1 (en) * | 2008-02-18 | 2009-08-27 | Genetic Technologies Limited | Cell processing and/or enrichment methods |
WO2010012002A1 (en) * | 2008-07-25 | 2010-01-28 | Saryna Medical Corporation | Methods and systems for genetic analysis of fetal nucleated red blood cells |
CA2737116C (en) * | 2008-09-16 | 2019-01-15 | Historx, Inc. | Reproducible quantification of biomarker expression |
US20100167271A1 (en) * | 2008-12-30 | 2010-07-01 | Streck, Inc. | Method for screening blood using a preservative that may be in a substantially solid state form |
US11634747B2 (en) * | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
WO2010085815A1 (en) * | 2009-01-26 | 2010-07-29 | Artemis Health, Inc. | Methods and compositions for identifying a fetal cell |
US8231007B2 (en) * | 2009-01-29 | 2012-07-31 | Wark Rickey E | Static classifier cage |
DE202010018569U1 (de) | 2009-02-18 | 2017-09-26 | Streck Inc. | Konservierung zellfreier Nukleinsäuren |
CA2795268A1 (en) * | 2009-04-21 | 2010-10-28 | Genetic Technologies Limited | Methods for obtaining fetal genetic material |
CA2765779A1 (en) * | 2009-06-24 | 2010-12-29 | Fund For Medical Research Development Of Infrastructure And Health Servi Ces - At Rambam Medical Center | Methods and kits for isolating placental derived microparticles and use of same for diagnosis of fetal disorders |
ES2571104T3 (es) * | 2009-11-09 | 2016-05-24 | Streck Inc | Estabilización del ARN y extracción del ARN presente en células intactas dentro de una muestra de sangre |
CN102762712A (zh) | 2010-01-21 | 2012-10-31 | 百赛普有限公司 | 稀有细胞的磁性分离 |
US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
US20130034853A1 (en) * | 2010-04-20 | 2013-02-07 | Brian Kelly | Two-color chromogenic in situ hybridization |
WO2012151391A2 (en) | 2011-05-04 | 2012-11-08 | Streck, Inc. | Inactivated virus compositions and methods of preparing such compositions |
KR101981450B1 (ko) * | 2011-12-23 | 2019-08-28 | 디퍼이 신테스 프로덕츠, 인코포레이티드 | 인간 제대 조직-유래된 세포의 검출 |
CN104508122A (zh) * | 2012-06-20 | 2015-04-08 | 赛璐拉有限公司 | 用于处理细胞群体的材料和方法 |
EP4008270A1 (en) | 2012-06-22 | 2022-06-08 | Preprogen LLC | Method for obtaining fetal cells and fetal cellular components |
CN109554350B (zh) * | 2012-11-27 | 2022-09-23 | 儿童医疗中心有限公司 | 用于胎儿血红蛋白再诱导的靶向bcl11a远端调控元件 |
WO2014160891A1 (en) | 2013-03-27 | 2014-10-02 | Theranos, Inc. | Methods, devices, and systems for sample analysis |
WO2015013244A1 (en) | 2013-07-24 | 2015-01-29 | Streck, Inc. | Compositions and methods for stabilizing circulating tumor cells |
US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
CN104977284B (zh) * | 2015-07-03 | 2018-05-29 | 石莹 | 一种胎儿有核红细胞的捕获及鉴定方法 |
KR101794218B1 (ko) * | 2015-08-19 | 2017-11-07 | 인제대학교 산학협력단 | 비침습적 산전 진단을 위한 산모 혈액 내 유핵 적혈구의 농축 분리 방법 |
WO2017057076A1 (ja) * | 2015-09-28 | 2017-04-06 | 富士フイルム株式会社 | 有核赤血球の取得方法および有核赤血球の識別方法 |
US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
US11506655B2 (en) | 2016-07-29 | 2022-11-22 | Streck, Inc. | Suspension composition for hematology analysis control |
JP6234542B1 (ja) | 2016-12-27 | 2017-11-22 | 株式会社 TL Genomics | 胎児細胞由来染色体dnaの取得方法 |
US11666915B2 (en) | 2017-10-19 | 2023-06-06 | Tl Genomics Inc. | Cell classification chip |
CN109115584A (zh) * | 2018-09-10 | 2019-01-01 | 首都医科大学附属北京妇产医院 | 一种检测红细胞中血红蛋白f的检测试剂及其应用 |
KR20230114327A (ko) * | 2019-07-15 | 2023-08-01 | 메나리니 바이오마커스 싱가포르 피티이 리미티드 | 태아 세포의 단리, 검출 및 분석을 위한 조성물 및방법 |
CN111948404B (zh) * | 2020-08-03 | 2023-04-07 | 融智生物科技(青岛)有限公司 | 用于筛查地中海贫血的特征蛋白标记组合物、质谱模型及其应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0500727B1 (en) * | 1989-11-13 | 1998-01-21 | Children's Medical Center Corporation | Non-invasive method for isolation and detection of fetal dna |
CA2140278C (en) * | 1992-07-17 | 2009-03-17 | Morteza Asgari | Enriching and identyfing fetal cells in maternal blood for in situ hybridization |
US5457024A (en) * | 1993-01-22 | 1995-10-10 | Aprogenex, Inc. | Isolation of fetal erythrocytes |
WO1995003431A1 (en) * | 1993-07-19 | 1995-02-02 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
US5580724A (en) * | 1994-03-25 | 1996-12-03 | Board Of Regents, The University Of Texas System | Differential expansion of fetal stem cells in maternal circulation for use in prenatal genetic analysis |
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- 1996-10-21 US US08/734,556 patent/US5731156A/en not_active Expired - Lifetime
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- 1997-10-20 WO PCT/US1997/019447 patent/WO1998018005A1/en active IP Right Grant
- 1997-10-20 AU AU49200/97A patent/AU735380B2/en not_active Ceased
- 1997-10-20 KR KR10-1999-7003439A patent/KR100523381B1/ko not_active IP Right Cessation
- 1997-10-20 EP EP97911938A patent/EP1007965B1/en not_active Expired - Lifetime
- 1997-10-20 DE DE69732662T patent/DE69732662T2/de not_active Expired - Fee Related
- 1997-10-20 CN CN97199005A patent/CN1234117A/zh active Pending
- 1997-10-20 JP JP51969498A patent/JP4091123B2/ja not_active Expired - Fee Related
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- 1997-10-20 AT AT97911938T patent/ATE290210T1/de not_active IP Right Cessation
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EP1007965A4 (en) | 2000-06-14 |
AU4920097A (en) | 1998-05-15 |
DE69732662T2 (de) | 2005-12-29 |
EP1007965B1 (en) | 2005-03-02 |
EP1007965A1 (en) | 2000-06-14 |
JP2001502805A (ja) | 2001-02-27 |
KR100523381B1 (ko) | 2005-10-24 |
AU735380B2 (en) | 2001-07-05 |
CA2269327A1 (en) | 1998-04-30 |
CN1234117A (zh) | 1999-11-03 |
RU2178703C2 (ru) | 2002-01-27 |
ATE290210T1 (de) | 2005-03-15 |
HK1031255A1 (en) | 2001-06-08 |
IL129382A0 (en) | 2000-02-17 |
DE69732662D1 (de) | 2005-04-07 |
KR20000052662A (ko) | 2000-08-25 |
US5731156A (en) | 1998-03-24 |
JP4091123B2 (ja) | 2008-05-28 |
WO1998018005A1 (en) | 1998-04-30 |
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