IL129382A - Method for identifying embryonic cells using antibodies against fetal hemoglobin - Google Patents

Method for identifying embryonic cells using antibodies against fetal hemoglobin

Info

Publication number: IL129382A
Authority: IL; Israel
Prior art keywords: fetal; antibody; cells; hemoglobin; cell
Prior art date: 1996-10-21

Application number

IL12938297A

Other languages

English (en)

Hebrew (he)

Other versions

IL129382A0 (en

Original Assignee

Applied Imaging Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-10-21

Filing date

1997-10-20

Publication date

2002-09-12

1997-10-20 Application filed by Applied Imaging Inc filed Critical Applied Imaging Inc

1997-10-20 Priority to IL14569197A priority Critical patent/IL145691A/xx

2000-02-17 Publication of IL129382A0 publication Critical patent/IL129382A0/xx

2002-09-12 Publication of IL129382A publication Critical patent/IL129382A/en

Links

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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit

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Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
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Biomedical Technology (AREA)
Hematology (AREA)
Molecular Biology (AREA)
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General Physics & Mathematics (AREA)
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Biochemistry (AREA)
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Food Science & Technology (AREA)
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Medicinal Chemistry (AREA)
Biotechnology (AREA)
Cell Biology (AREA)
Pregnancy & Childbirth (AREA)
Reproductive Health (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gynecology & Obstetrics (AREA)
Dispersion Chemistry (AREA)
Investigating Or Analysing Biological Materials (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

IL12938297A 1996-10-21 1997-10-20 Method for identifying embryonic cells using antibodies against fetal hemoglobin IL129382A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
IL14569197A IL145691A (en)	1996-10-21	1997-10-20	Method to identify fetal cells using anti-embryonic hemoglobin antibodies

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US08/734,556 US5731156A (en)	1996-10-21	1996-10-21	Use of anti-embryonic hemoglobin antibodies to identify fetal cells
PCT/US1997/019447 WO1998018005A1 (en)	1996-10-21	1997-10-20	Use of anti-embryonic hemoglobin antibodies to identify fetal cells

Publications (2)

Publication Number	Publication Date
IL129382A0 IL129382A0 (en)	2000-02-17
IL129382A true IL129382A (en)	2002-09-12

Family

ID=24952172

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL12938297A IL129382A (en)	1996-10-21	1997-10-20	Method for identifying embryonic cells using antibodies against fetal hemoglobin

Country Status (14)

Country	Link
US (1)	US5731156A (ru)
EP (1)	EP1007965B1 (ru)
JP (1)	JP4091123B2 (ru)
KR (1)	KR100523381B1 (ru)
CN (1)	CN1234117A (ru)
AT (1)	ATE290210T1 (ru)
AU (1)	AU735380B2 (ru)
BR (1)	BR9712548A (ru)
CA (1)	CA2269327A1 (ru)
DE (1)	DE69732662T2 (ru)
HK (1)	HK1031255A1 (ru)
IL (1)	IL129382A (ru)
RU (1)	RU2178703C2 (ru)
WO (1)	WO1998018005A1 (ru)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6509192B1 (en) *	1992-02-24	2003-01-21	Coulter International Corp.	Quality control method
US5629147A (en) *	1992-07-17	1997-05-13	Aprogenex, Inc.	Enriching and identifying fetal cells in maternal blood for in situ hybridization
US6718053B1 (en) *	1996-11-27	2004-04-06	Chromavision Medical Systems, Inc.	Method and apparatus for automated image analysis of biological specimens
CA2236268A1 (en)	1995-11-30	1997-06-05	Chromavision Medical Systems, Inc.	Method and apparatus for automated image analysis of biological specimens
PL335543A1 (en) *	1997-03-08	2000-04-25	Univ Dundee	Prenatal diagnosing methods
US5962234A (en) *	1997-10-20	1999-10-05	Applied Imaging Corporation	Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells
CA2331508A1 (en) *	1998-05-09	1999-11-18	Ikonisys, Inc.	Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis
FR2781055B1 (fr) *	1998-07-09	2000-10-13	Immunotech Sa	Reactif et methode pour la permeabilisation et l'identification des erythrocytes
CA2413835A1 (en) *	1999-05-25	2000-11-30	Britta Christensen	Isolation and culturing of fetal cells
US6692952B1 (en) *	1999-11-10	2004-02-17	Massachusetts Institute Of Technology	Cell analysis and sorting apparatus for manipulation of cells
DE10035433C2 (de) *	2000-07-20	2002-07-18	Tuma Wolfgang	Schonende Hochanreicherung von fetalen Zellen aus pripherem Blut und Verwendung derselben
AU2002357588B2 (en) *	2001-12-11	2008-11-20	Netech Inc.	Blood cell separation system
WO2004029221A2 (en)	2002-09-27	2004-04-08	The General Hospital Corporation	Microfluidic device for cell separation and uses thereof
DK1816461T3 (da)	2002-10-16	2020-04-14	Streck Laboratories Inc	Fremgangsmåde og indretning til indsamling og sikring af celler til brug for analyse
US20040214243A1 (en) *	2003-04-25	2004-10-28	Beckman Coulter, Inc.	Differential determination of hemoglobins
US20070160503A1 (en) *	2003-06-13	2007-07-12	Palaniappan Sethu	Microfluidic systems for size based removal of red blood cells and platelets from blood
WO2005084374A2 (en) *	2004-03-03	2005-09-15	The General Hospital Corporation	Magnetic device for isolation of cells and biomolecules in a microfluidic environment
US7653260B2 (en) *	2004-06-17	2010-01-26	Carl Zeis MicroImaging GmbH	System and method of registering field of view
US8582924B2 (en) *	2004-06-30	2013-11-12	Carl Zeiss Microimaging Gmbh	Data structure of an image storage and retrieval system
US20070026415A1 (en) *	2005-07-29	2007-02-01	Martin Fuchs	Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026417A1 (en) *	2005-07-29	2007-02-01	Martin Fuchs	Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070196820A1 (en)	2005-04-05	2007-08-23	Ravi Kapur	Devices and methods for enrichment and alteration of cells and other particles
US20070026414A1 (en) *	2005-07-29	2007-02-01	Martin Fuchs	Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026413A1 (en) *	2005-07-29	2007-02-01	Mehmet Toner	Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20090181421A1 (en) *	2005-07-29	2009-07-16	Ravi Kapur	Diagnosis of fetal abnormalities using nucleated red blood cells
US20070026416A1 (en) *	2005-07-29	2007-02-01	Martin Fuchs	Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8921102B2 (en)	2005-07-29	2014-12-30	Gpb Scientific, Llc	Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070059680A1 (en) *	2005-09-15	2007-03-15	Ravi Kapur	System for cell enrichment
US20070059719A1 (en) *	2005-09-15	2007-03-15	Michael Grisham	Business methods for prenatal Diagnosis
US20070059683A1 (en) *	2005-09-15	2007-03-15	Tom Barber	Veterinary diagnostic system
US20070059774A1 (en) *	2005-09-15	2007-03-15	Michael Grisham	Kits for Prenatal Testing
US20070059716A1 (en) *	2005-09-15	2007-03-15	Ulysses Balis	Methods for detecting fetal abnormality
US20070059781A1 (en) *	2005-09-15	2007-03-15	Ravi Kapur	System for size based separation and analysis
US20070059718A1 (en) *	2005-09-15	2007-03-15	Mehmet Toner	Systems and methods for enrichment of analytes
EP2156370B1 (en) *	2007-05-14	2011-10-12	Historx, Inc.	Compartment segregation by pixel characterization using image data clustering
JP5593221B2 (ja) *	2007-06-15	2014-09-17	ヒストロックス，インコーポレイテッド．	顕微鏡機器を標準化するための方法およびシステム
CA2604317C (en)	2007-08-06	2017-02-28	Historx, Inc.	Methods and system for validating sample images for quantitative immunoassays
CA2596204C (en) *	2007-08-07	2019-02-26	Historx, Inc.	Method and system for determining an optimal dilution of a reagent
WO2009029810A1 (en) *	2007-08-31	2009-03-05	Historx, Inc.	Automatic exposure time selection for imaging tissue
EP2232266B1 (en)	2007-12-20	2012-12-05	Cytyc Corporation	Method for magnetic separation of red blood cells from a patient sample
WO2009103110A1 (en) *	2008-02-18	2009-08-27	Genetic Technologies Limited	Cell processing and/or enrichment methods
WO2010012002A1 (en) *	2008-07-25	2010-01-28	Saryna Medical Corporation	Methods and systems for genetic analysis of fetal nucleated red blood cells
CA2737116C (en) *	2008-09-16	2019-01-15	Historx, Inc.	Reproducible quantification of biomarker expression
US20100167271A1 (en) *	2008-12-30	2010-07-01	Streck, Inc.	Method for screening blood using a preservative that may be in a substantially solid state form
US11634747B2 (en) *	2009-01-21	2023-04-25	Streck Llc	Preservation of fetal nucleic acids in maternal plasma
WO2010085815A1 (en) *	2009-01-26	2010-07-29	Artemis Health, Inc.	Methods and compositions for identifying a fetal cell
US8231007B2 (en) *	2009-01-29	2012-07-31	Wark Rickey E	Static classifier cage
DE202010018569U1 (de)	2009-02-18	2017-09-26	Streck Inc.	Konservierung zellfreier Nukleinsäuren
CA2795268A1 (en) *	2009-04-21	2010-10-28	Genetic Technologies Limited	Methods for obtaining fetal genetic material
CA2765779A1 (en) *	2009-06-24	2010-12-29	Fund For Medical Research Development Of Infrastructure And Health Servi Ces - At Rambam Medical Center	Methods and kits for isolating placental derived microparticles and use of same for diagnosis of fetal disorders
ES2571104T3 (es) *	2009-11-09	2016-05-24	Streck Inc	Estabilización del ARN y extracción del ARN presente en células intactas dentro de una muestra de sangre
CN102762712A (zh)	2010-01-21	2012-10-31	百赛普有限公司	稀有细胞的磁性分离
US8774488B2 (en)	2010-03-11	2014-07-08	Cellscape Corporation	Method and device for identification of nucleated red blood cells from a maternal blood sample
US20130034853A1 (en) *	2010-04-20	2013-02-07	Brian Kelly	Two-color chromogenic in situ hybridization
WO2012151391A2 (en)	2011-05-04	2012-11-08	Streck, Inc.	Inactivated virus compositions and methods of preparing such compositions
KR101981450B1 (ko) *	2011-12-23	2019-08-28	디퍼이 신테스 프로덕츠, 인코포레이티드	인간 제대 조직-유래된 세포의 검출
CN104508122A (zh) *	2012-06-20	2015-04-08	赛璐拉有限公司	用于处理细胞群体的材料和方法
EP4008270A1 (en)	2012-06-22	2022-06-08	Preprogen LLC	Method for obtaining fetal cells and fetal cellular components
CN109554350B (zh) *	2012-11-27	2022-09-23	儿童医疗中心有限公司	用于胎儿血红蛋白再诱导的靶向bcl11a远端调控元件
WO2014160891A1 (en)	2013-03-27	2014-10-02	Theranos, Inc.	Methods, devices, and systems for sample analysis
WO2015013244A1 (en)	2013-07-24	2015-01-29	Streck, Inc.	Compositions and methods for stabilizing circulating tumor cells
US11168351B2 (en)	2015-03-05	2021-11-09	Streck, Inc.	Stabilization of nucleic acids in urine
CN104977284B (zh) *	2015-07-03	2018-05-29	石莹	一种胎儿有核红细胞的捕获及鉴定方法
KR101794218B1 (ko) *	2015-08-19	2017-11-07	인제대학교 산학협력단	비침습적 산전 진단을 위한 산모 혈액 내 유핵 적혈구의 농축 분리 방법
WO2017057076A1 (ja) *	2015-09-28	2017-04-06	富士フイルム株式会社	有核赤血球の取得方法および有核赤血球の識別方法
US20170145475A1 (en)	2015-11-20	2017-05-25	Streck, Inc.	Single spin process for blood plasma separation and plasma composition including preservative
US11506655B2 (en)	2016-07-29	2022-11-22	Streck, Inc.	Suspension composition for hematology analysis control
JP6234542B1 (ja)	2016-12-27	2017-11-22	株式会社ＴＬＧｅｎｏｍｉｃｓ	胎児細胞由来染色体ｄｎａの取得方法
US11666915B2 (en)	2017-10-19	2023-06-06	Tl Genomics Inc.	Cell classification chip
CN109115584A (zh) *	2018-09-10	2019-01-01	首都医科大学附属北京妇产医院	一种检测红细胞中血红蛋白f的检测试剂及其应用
KR20230114327A (ko) *	2019-07-15	2023-08-01	메나리니 바이오마커스 싱가포르 피티이 리미티드	태아 세포의 단리, 검출 및 분석을 위한 조성물 및방법
CN111948404B (zh) *	2020-08-03	2023-04-07	融智生物科技（青岛）有限公司	用于筛查地中海贫血的特征蛋白标记组合物、质谱模型及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0500727B1 (en) *	1989-11-13	1998-01-21	Children's Medical Center Corporation	Non-invasive method for isolation and detection of fetal dna
CA2140278C (en) *	1992-07-17	2009-03-17	Morteza Asgari	Enriching and identyfing fetal cells in maternal blood for in situ hybridization
US5457024A (en) *	1993-01-22	1995-10-10	Aprogenex, Inc.	Isolation of fetal erythrocytes
WO1995003431A1 (en) *	1993-07-19	1995-02-02	Aprogenex, Inc.	Enriching and identifying fetal cells in maternal blood for in situ hybridization
US5580724A (en) *	1994-03-25	1996-12-03	Board Of Regents, The University Of Texas System	Differential expansion of fetal stem cells in maternal circulation for use in prenatal genetic analysis

1996
- 1996-10-21 US US08/734,556 patent/US5731156A/en not_active Expired - Lifetime
1997
- 1997-10-20 WO PCT/US1997/019447 patent/WO1998018005A1/en active IP Right Grant
- 1997-10-20 AU AU49200/97A patent/AU735380B2/en not_active Ceased
- 1997-10-20 KR KR10-1999-7003439A patent/KR100523381B1/ko not_active IP Right Cessation
- 1997-10-20 EP EP97911938A patent/EP1007965B1/en not_active Expired - Lifetime
- 1997-10-20 DE DE69732662T patent/DE69732662T2/de not_active Expired - Fee Related
- 1997-10-20 CN CN97199005A patent/CN1234117A/zh active Pending
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BR9712548A (pt)	1999-12-21
EP1007965A4 (en)	2000-06-14
AU4920097A (en)	1998-05-15
DE69732662T2 (de)	2005-12-29
EP1007965B1 (en)	2005-03-02
EP1007965A1 (en)	2000-06-14
JP2001502805A (ja)	2001-02-27
KR100523381B1 (ko)	2005-10-24
AU735380B2 (en)	2001-07-05
CA2269327A1 (en)	1998-04-30
CN1234117A (zh)	1999-11-03
RU2178703C2 (ru)	2002-01-27
ATE290210T1 (de)	2005-03-15
HK1031255A1 (en)	2001-06-08
IL129382A0 (en)	2000-02-17
DE69732662D1 (de)	2005-04-07
KR20000052662A (ko)	2000-08-25
US5731156A (en)	1998-03-24
JP4091123B2 (ja)	2008-05-28
WO1998018005A1 (en)	1998-04-30

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Publication	Publication Date	Title
AU735380B2 (en)	2001-07-05	Use of anti-embryonic hemoglobin antibodies to identify fetal cells
US5962234A (en)	1999-10-05	Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells
CA2140278C (en)	2009-03-17	Enriching and identyfing fetal cells in maternal blood for in situ hybridization
US5714325A (en)	1998-02-03	Prenatal diagnosis by isolation of fetal granulocytes from maternal blood
US5153117A (en)	1992-10-06	Fetal cell recovery method
EP0500727B1 (en)	1998-01-21	Non-invasive method for isolation and detection of fetal dna
US6210889B1 (en)	2001-04-03	Method for enrichment of fetal cells from maternal blood and use of same in determination of fetal sex and detection of chromosomal abnormalities
US5861311A (en)	1999-01-19	Preserved, non-infectious control cells prepared by the modulation or modification of normal leukocytes
Davis et al.	1995	Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules
JPH09510875A (ja)	1997-11-04	母親の末梢血からの胎児細胞の培養および単離
WO1994025873A1 (en)	1994-11-10	Methods for enriching fetal progenitor cells from maternal blood
EP0660664B1 (en)	2002-04-17	Preserved, non-infectious control cells for use in the identification of a disease through blood testing
Christensen et al.	2003	Studies on the isolation and identification of fetal nucleated red blood cells in the circulation of pregnant women before and after chorion villus sampling
US5763204A (en)	1998-06-09	Preparation of preserved, non-infectious control cell for use in the identification of a disease through blood testing
MXPA99003697A (en)	2000-06-01	Use of anti-embryonic hemoglobin antibodies to identify fetal cells
Bischoff et al.	2003	Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood
AU9718698A (en)	1999-03-04	Prenatal diagnosis by isolation of fetal granulocytes from maternal blood
AU7735201A (en)	2003-04-03	Fetal cell recovery method