IL110238A - Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide - Google Patents

Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide

Info

Publication number
IL110238A
IL110238A IL11023894A IL11023894A IL110238A IL 110238 A IL110238 A IL 110238A IL 11023894 A IL11023894 A IL 11023894A IL 11023894 A IL11023894 A IL 11023894A IL 110238 A IL110238 A IL 110238A
Authority
IL
Israel
Prior art keywords
sequence encoding
dna sequence
signal peptide
pct
dna
Prior art date
Application number
IL11023894A
Other languages
English (en)
Other versions
IL110238A0 (en
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of IL110238A0 publication Critical patent/IL110238A0/xx
Publication of IL110238A publication Critical patent/IL110238A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8103—Exopeptidase (E.C. 3.4.11-19) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
IL11023894A 1993-07-08 1994-07-07 Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide IL110238A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK93828A DK82893D0 (da) 1993-07-08 1993-07-08 Peptid

Publications (2)

Publication Number Publication Date
IL110238A0 IL110238A0 (en) 1994-10-21
IL110238A true IL110238A (en) 2003-04-10

Family

ID=8098010

Family Applications (1)

Application Number Title Priority Date Filing Date
IL11023894A IL110238A (en) 1993-07-08 1994-07-07 Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide

Country Status (20)

Country Link
US (1) US5726038A (de)
EP (1) EP0792367B1 (de)
JP (1) JP3542604B2 (de)
KR (1) KR100245173B1 (de)
AT (1) ATE207124T1 (de)
AU (1) AU7122194A (de)
CA (1) CA2166684A1 (de)
DE (1) DE69428719T2 (de)
DK (2) DK82893D0 (de)
ES (1) ES2165881T3 (de)
HU (1) HU222406B1 (de)
IL (1) IL110238A (de)
NZ (1) NZ268346A (de)
PL (1) PL178924B1 (de)
PT (1) PT792367E (de)
RU (1) RU2143495C1 (de)
SG (1) SG52452A1 (de)
UA (1) UA40625C2 (de)
WO (1) WO1995002059A1 (de)
ZA (1) ZA944912B (de)

Families Citing this family (42)

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FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
US5861267A (en) * 1995-05-01 1999-01-19 Vertex Pharmaceuticals Incorporated Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
US5772629A (en) * 1995-10-23 1998-06-30 Localmed, Inc. Localized intravascular delivery of TFPI for inhibition of restenosis in recanalized blood vessels
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
JP4275741B2 (ja) * 1996-12-13 2009-06-10 ノバルティス バクシンズ アンド ダイアグノスティックス， インコーポレーテッド 血小板由来増殖因子タンパク質の分析および分離
WO1998032867A1 (en) * 1997-01-24 1998-07-30 Novo Nordisk A/S Synthetic leader peptide sequences
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
WO2000066709A2 (en) * 1999-04-30 2000-11-09 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
DE60044728D1 (de) 1999-12-29 2010-09-02 Novo Nordisk As VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
CA2405709A1 (en) * 2000-04-12 2001-10-25 Human Genome Sciences, Inc. Albumin fusion proteins
US20030143191A1 (en) * 2001-05-25 2003-07-31 Adam Bell Chemokine beta-1 fusion proteins
CA2471363C (en) * 2001-12-21 2014-02-11 Human Genome Sciences, Inc. Albumin fusion proteins
EP1463752A4 (de) * 2001-12-21 2005-07-13 Human Genome Sciences Inc Albuminfusionsproteine
AU2003237061A1 (en) * 2002-06-29 2004-01-19 Bioholdings Co., Ltd Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same
WO2004024885A2 (en) 2002-09-13 2004-03-25 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
KR100470978B1 (ko) * 2003-01-02 2005-03-10 한국생명공학연구원 앱신 다중 결손 효모 변이 균주 및 이를 이용한 재조합 단백질의 생산 방법
JP2007532096A (ja) 2003-11-14 2007-11-15 ノボ　ノルディスク　アクティーゼルスカブ アシル化されたインスリンの製造方法
WO2005054291A1 (en) 2003-12-03 2005-06-16 Novo Nordisk A/S Single-chain insulin
PL1729795T3 (pl) * 2004-02-09 2016-08-31 Human Genome Sciences Inc Białka fuzyjne albuminy
WO2007020256A1 (en) 2005-08-16 2007-02-22 Novo Nordisk A/S Method for making mature insulin polypeptides
JP5202338B2 (ja) 2006-02-27 2013-06-05 ノボ・ノルデイスク・エー／エス 新規インシュリン誘導体
EP2069502B1 (de) 2006-09-27 2014-02-26 Novo Nordisk A/S Verfahren zur herstellung gereifter insulinpolypeptide
WO2008062010A2 (en) 2006-11-22 2008-05-29 Novo Nordisk A/S Method for making activated carboxypeptidases
JP5688969B2 (ja) 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス プロテアーゼに対して安定しているペグ化インスリンアナログ
MX2010009850A (es) 2008-03-18 2010-09-30 Novo Nordisk As Analogos de insulina acilados y etabilizados contra proteasas.
EP2258855A1 (de) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expressionssequenzen
CN105821078A (zh) 2010-12-09 2016-08-03 巴斯德研究所 用于获得高产量重组蛋白表达的基于mgmt的方法
DE102011007313A1 (de) * 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
DE102011118032A1 (de) * 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
AU2012350229B2 (en) 2011-12-09 2018-03-22 Institut Pasteur Multiplex immuno screening assay
CA2886460C (en) 2012-10-29 2023-11-14 Lonza Ltd Leader peptides for expressing a protein of interest in an eukaryotic host cell
WO2014088836A1 (en) 2012-12-03 2014-06-12 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
EP3004156A1 (de) 2013-06-07 2016-04-13 Novo Nordisk A/S Verfahren zur herstellung von reifen insulinpolypeptiden
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka
WO2016144658A1 (en) 2015-03-10 2016-09-15 Merck Sharp & Dohme Corp. Process for preparing recombinant insulin using microfiltration
EA037848B1 (ru) 2016-07-14 2021-05-27 Общество С Ограниченной Ответственностью "Биохимический Агент" Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты)
WO2019099615A1 (en) 2017-11-17 2019-05-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Use of copeptin to modulate the immune response and hematopoiesis, and stimulate bone growth
WO2022049409A1 (en) 2020-09-02 2022-03-10 Sia Terragen Express diagnosticum for sars-cov-2
CN112920280B (zh) * 2021-04-22 2022-07-05 江南大学 一种酸性蛋白酶高效表达的方法及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987002670A1 (en) * 1985-10-25 1987-05-07 Mackay Vivian L Method of using bar1 for secreting foreign proteins
US5217891A (en) * 1987-07-28 1993-06-08 Chiron Corporation DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides
US5037743A (en) * 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide

Also Published As

Publication number Publication date
HU9600053D0 (en) 1996-03-28
KR100245173B1 (en) 2000-02-15
PT792367E (pt) 2002-04-29
JPH08512202A (ja) 1996-12-24
DE69428719D1 (de) 2001-11-22
ATE207124T1 (de) 2001-11-15
CA2166684A1 (en) 1995-01-19
SG52452A1 (en) 1998-09-28
ZA944912B (en) 1995-03-27
AU7122194A (en) 1995-02-06
ES2165881T3 (es) 2002-04-01
JP3542604B2 (ja) 2004-07-14
IL110238A0 (en) 1994-10-21
HU222406B1 (hu) 2003-06-28
NZ268346A (en) 1997-05-26
DE69428719T2 (de) 2002-08-08
EP0792367A1 (de) 1997-09-03
US5726038A (en) 1998-03-10
UA40625C2 (uk) 2001-08-15
DK82893D0 (da) 1993-07-08
HUT74253A (en) 1996-11-28
DK0792367T3 (da) 2001-12-31
EP0792367B1 (de) 2001-10-17
PL312436A1 (en) 1996-04-29
RU2143495C1 (ru) 1999-12-27
PL178924B1 (pl) 2000-06-30
WO1995002059A1 (en) 1995-01-19

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Legal Events

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MM9K Patent not in force due to non-payment of renewal fees