HRP20210325T1 - Postupak za enzimatsku hidrolizu u uzastopnom postupku dodavanja uz postupno odvojeno dodavanje prethodno obrađenog supstrata tijekom vremena - Google Patents
Postupak za enzimatsku hidrolizu u uzastopnom postupku dodavanja uz postupno odvojeno dodavanje prethodno obrađenog supstrata tijekom vremena Download PDFInfo
- Publication number
- HRP20210325T1 HRP20210325T1 HRP20210325TT HRP20210325T HRP20210325T1 HR P20210325 T1 HRP20210325 T1 HR P20210325T1 HR P20210325T T HRP20210325T T HR P20210325TT HR P20210325 T HRP20210325 T HR P20210325T HR P20210325 T1 HRP20210325 T1 HR P20210325T1
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- Croatia
- Prior art keywords
- addition
- impeller
- previous patent
- reactor
- over time
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 23
- 239000000758 substrate Substances 0.000 title claims 8
- 230000007071 enzymatic hydrolysis Effects 0.000 title claims 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title claims 2
- 238000007792 addition Methods 0.000 claims 10
- 102000004190 Enzymes Human genes 0.000 claims 6
- 108090000790 Enzymes Proteins 0.000 claims 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- 238000000855 fermentation Methods 0.000 claims 2
- 230000004151 fermentation Effects 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (17)
1. Postupak za enzimatsku hidrolizu uzastopnim dodavanjem u kojem se, uz miješanje, najmanje jedan prethodno obrađeni lignocelulozni supstrat dovodi u kontakt s vodom i enzimima u reaktoru, pri čemu je navedeni postupak naznačen time što se provodi uzastopno dodavanje u reaktor prethodno obrađenog lignoceluloznog supstrata i s vremenom je dodavanje sve više razmaknuto, tako da se dobije unaprijed određeni konačni sadržaj suhe tvari od između 18 i 30 masenih%.
2. Postupak u skladu s patentnim zahtjevom 1, naznačen time što je konačni udio krutina iznosi između 18 i 24 masenih%.
3. Postupak prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time što se provode najmanje 4 razmaknuta dodavanja prethodno obrađenog lignoceluloznog supstrata, poželjno najmanje 5 dodavanja.
4. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što količina lignoceluloznog supstrata dodana tijekom dodavanja predstavlja između 2 i 3 masenih% suhe tvari.
5. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što se dodavanje enzima u reaktor provodi uzastopno i vremenom se dodavanje sve više odvaja.
6. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time da se prethodno obrađeni lignocelulozni supstrat i enzimi dodaju istovremeno u reaktor.
7. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što se pri svakom dodavanju, prethodno obrađeni lignocelulozni supstrat dodaje u istoj količini.
8. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što se pri svakom dodavanju, enzimi dodaju u istoj količini.
9. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što reaktor sadrži impeler i omjer D/T promjera impelera / promjer reaktora iznosi između 0,3 i 0,75.
10. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što je impeler, impeler sa zakošenim lopaticama ili impeler tipa brodskog propelera.
11. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što reaktor sadrži impeler koji pokreće motor i time što se tijekom vremena provodi mjerenje električne energije koju troši motor.
12. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što reaktor sadrži impeler koji pokreće motor i time što je brzina vrtnje impelera manja od 100 okr/min, poželjno manja od 80 okr/min.
13. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što se enzimi dovode u kontakt u koncentraciji od između 0,1 i 60 mg enzima po gramu celuloze.
14. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što se postupak provodi na temperaturi od između 40°C i 60°C, uz pH od između 4 i 6, i na atmosferskom tlaku.
15. Postupak prema jednom od prethodnih patentnih zahtjeva, što se koriste različiti prethodno obrađeni lignocelulozni supstrati, sami ili kao smjesa.
16. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što navedeni postupak slijedi korak fermentacije u prisutnosti mikroorganizma koji proizvodi alkohol.
17. Postupak prema jednom od prethodnih patentnih zahtjeva, naznačen time što se navedeni postupak provodi u prisutnosti mikroorganizma koji proizvodi alkohol prema postupku istodobne saharifikacije i fermentacije.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1759033A FR3071510B1 (fr) | 2017-09-28 | 2017-09-28 | Procede d'hydrolyse enzymatique en alimentation sequentielle avec ajouts du substrat pretraite de plus en plus espaces dans le temps |
| EP18191590.1A EP3461902B2 (fr) | 2017-09-28 | 2018-08-30 | Procédé d'hydrolyse enzymatique en alimentation séquentielle avec ajouts du substrat prétraité de plus en plus éspaces dans le temps |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20210325T1 true HRP20210325T1 (hr) | 2021-04-02 |
Family
ID=60765821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20210325TT HRP20210325T1 (hr) | 2017-09-28 | 2021-02-25 | Postupak za enzimatsku hidrolizu u uzastopnom postupku dodavanja uz postupno odvojeno dodavanje prethodno obrađenog supstrata tijekom vremena |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10865426B2 (hr) |
| EP (1) | EP3461902B2 (hr) |
| CN (1) | CN109576323B (hr) |
| BR (1) | BR102018069236A2 (hr) |
| CA (1) | CA3018686A1 (hr) |
| DK (1) | DK3461902T4 (hr) |
| ES (1) | ES2852425T5 (hr) |
| FI (1) | FI3461902T4 (hr) |
| FR (1) | FR3071510B1 (hr) |
| HR (1) | HRP20210325T1 (hr) |
| HU (1) | HUE053210T2 (hr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3140633A1 (fr) | 2022-10-10 | 2024-04-12 | IFP Energies Nouvelles | Procédé de conversion de biomasse lignocellulosique |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9862980B2 (en) | 2014-01-16 | 2018-01-09 | Department Of Biotechnology | Process for production of soluble sugars from biomass |
| FR3021975B1 (fr) * | 2014-06-05 | 2018-03-30 | Compagnie Industrielle De La Matiere Vegetale - Cimv | Procede de production, notamment d'ethanol, par hydrolyse enzymatique sequencee de la cellulose et des hemicelluloses d'une matiere premiere lignocellulosique |
| CN104651429B (zh) | 2015-02-03 | 2018-05-18 | 湖南农业大学 | 一种辐照预处理和分批补料实现木质纤维素高底物浓度酶水解的方法 |
-
2017
- 2017-09-28 FR FR1759033A patent/FR3071510B1/fr active Active
-
2018
- 2018-08-30 ES ES18191590T patent/ES2852425T5/es active Active
- 2018-08-30 FI FIEP18191590.1T patent/FI3461902T4/fi active
- 2018-08-30 HU HUE18191590A patent/HUE053210T2/hu unknown
- 2018-08-30 EP EP18191590.1A patent/EP3461902B2/fr active Active
- 2018-08-30 DK DK18191590.1T patent/DK3461902T4/da active
- 2018-09-21 BR BR102018069236-4A patent/BR102018069236A2/pt unknown
- 2018-09-25 CA CA3018686A patent/CA3018686A1/fr active Pending
- 2018-09-27 US US16/143,562 patent/US10865426B2/en active Active
- 2018-09-27 CN CN201811130203.7A patent/CN109576323B/zh active Active
-
2021
- 2021-02-25 HR HRP20210325TT patent/HRP20210325T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2852425T5 (es) | 2024-06-20 |
| EP3461902B1 (fr) | 2020-12-02 |
| CN109576323A (zh) | 2019-04-05 |
| CN109576323B (zh) | 2023-07-04 |
| EP3461902B2 (fr) | 2024-01-03 |
| EP3461902A1 (fr) | 2019-04-03 |
| DK3461902T4 (da) | 2024-03-25 |
| FR3071510A1 (fr) | 2019-03-29 |
| CA3018686A1 (fr) | 2019-03-28 |
| ES2852425T3 (es) | 2021-09-13 |
| FR3071510B1 (fr) | 2021-08-27 |
| US10865426B2 (en) | 2020-12-15 |
| DK3461902T3 (da) | 2021-02-22 |
| HUE053210T2 (hu) | 2021-06-28 |
| FI3461902T4 (fi) | 2024-04-02 |
| BR102018069236A2 (pt) | 2019-04-16 |
| US20190093133A1 (en) | 2019-03-28 |
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